CN107821538A

CN107821538A - A kind of hypoglycemic biscuit and preparation method thereof

Info

Publication number: CN107821538A
Application number: CN201711343389.XA
Authority: CN
Inventors: 罗植升
Original assignee: Liuzhou Fei Sheng Peng Science And Technology Co Ltd
Current assignee: Liuzhou Fei Sheng Peng Science And Technology Co Ltd
Priority date: 2017-12-15
Filing date: 2017-12-15
Publication date: 2018-03-23

Abstract

The invention belongs to food technology field, and in particular to a kind of hypoglycemic biscuit and preparation method thereof, biscuit include basestocks 70 85% and functional additive 15 30%；The basestocks include：Black sesame powder, celery, wheat flour, peanut powder, egg white, baking powder, maltitol, olive oil, salt solution, citric acid, water；The functional additive includes：Ampelopsis grossedentata extrat 10 18%, Chinese wistaria extract 10 20%, Wild jujube seeds extract 8 15%, strong wind seed extract 8 15%, capillary extract 5 10%, garlic P.E 5 10%, dendrobium candidum extract 5 10%, Fructus Setariae Germinatus extract 3 10%, Radix Sangusorbae extract 5 12%, Carlina acaulis extract 5 10%.The hypoglycemic biscuit of the present invention not only has soft mouthfeel, meets nutrition proportion as defined in food security, and the effect that takes care of health of energy assisting in reducing blood sugar, securely and reliably, without any side effects.

Description

A kind of hypoglycemic biscuit and preparation method thereof

【Technical field】

The invention belongs to food technology field, and in particular to a kind of hypoglycemic biscuit and preparation method thereof.

【Background technology】

Society with fast rhythm lives, and the operating pressure of keen competition, serious prestige is caused to the physical and mental health of modern The side of body, in addition, long-term diet is unreasonable, i.e.,：Smart grain substitutes coarse food grain completely, substantial amounts of three high (high fever, high protein, higher fatty acid), The agricultural chemicals that is remained in the intake of (preservative is more, flavoring agent is more, hormone is more) more than three food and food, chemical fertilizer stockpiling in vivo, Along with air, pollution of waterhead and amount of exercise are less than normal, so go down to make for a long time three high diseases (hypertension, hyperglycaemia, high fat of blood), Cardiovascular and cerebrovascular disease, cancer etc. so-called " modern civilization diseases " rapidly rise, and continuous rejuvenation, and serious jeopardizes the mankind Health and lives.Typically carried out for three high patients using the therapy of Chinese medicine, Western medicine, Chinese and Western medicine, insulin injection.Chinese medicine Smell is poor, and many people are reluctant long-term use；Even if taking, there's no such thing as a totally safe medicine, allow and also produce resisting psychology during people's long-term use. How the effect of Chinese medicine, to be performed to maximum and does not injure human body, and be obviously improved mouthfeel, enabled a person to receive, be that we are urgent Need to solve the problems, such as.Containing a large amount of safely and effectively active material health products inside Chinese medicine, can hypoglycemic significantly, be always People expect the key solved the problems, such as.Save alcohol, hot-tempered matter and soap formula etc. containing aliphatic acid, Bu Gu in many Chinese medicines, can reduce blood glucose, Purify the blood fat, reduces fat deposition in the blood vessels, available for treating atherosclerosis.In with the improvement of living standards, Medicine dietotherapy is increasingly favored by people.Chinese medicine based on tcm health preserving theory is the Chinese medicine and health food of raw material, is The characteristic of China's health food.In China, traditional Chinese medicine has long-term edible history, and " integration of drinking and medicinal herbs " is cultural of long standing and well established. Early in《The Yellow Emperor's Canon of Internal Medicine》In 13 contained first prescriptions, have 8 head be eat medicine and prescription.The Ming Dynasty《Compendium of Materia Medica》Even more include Food medicine and with product exceeded 500 kinds, abundant varieties of resources are provided for the dietary therapy of Chinese medicine.Therefore, Chinese herbal medicine is applied to food With biscuit, health food is fabricated to, is the reason for one of the present invention is important.

【The content of the invention】

It is an object of the invention to provide a kind of hypoglycemic biscuit and preparation method thereof, hypoglycemic biscuit of the invention is not Only there is soft mouthfeel, meet nutrition proportion as defined in food security, and the effect that takes care of health of energy assisting in reducing blood sugar, safely may be used Lean on, it is without any side effects.

To achieve these goals, the technical solution adopted by the present invention is as follows：

A kind of hypoglycemic biscuit, including basestocks 70-85% and functional additive 15-30%；The basestocks are pressed Include according to percetage by weight：Black sesame powder 1-8%, celery 1-5%, wheat flour 10-20%, peanut powder 5-12%, egg white 8-15%, Baking powder 10-15%, maltitol 8-15%, olive oil 10-15%, salt solution 5-8%, citric acid 0.5-1.0%, water 10- 15%；The functional additive includes：Ampelopsis grossedentata extrat 10-18%, Chinese wistaria extract 10-20%, Wild jujube seeds extract 8- 15%th, strong wind seed extract 8-15%, capillary extract 5-10%, garlic P.E 5-10%, dendrobium candidum extract 5- 10%th, Fructus Setariae Germinatus extract 3-10%, Radix Sangusorbae extract 5-12%, Carlina acaulis extract 5-10%.

Preferably, the basestocks include according to percetage by weight：Black sesame powder 5%, celery 3%, wheat flour 18%, peanut Powder 11.2%, egg white 10%, baking powder 12%, maltitol 10%, olive oil 13%, salt solution 5%, citric acid 0.8%, water 12%；The functional additive includes：Ampelopsis grossedentata extrat 14%, Chinese wistaria extract 15%, Wild jujube seeds extract 11%, strong wind Seed extract 12%, capillary extract 7%, garlic P.E 6%, dendrobium candidum extract 8%, Fructus Setariae Germinatus extract 8%, garden burnet Extract 10%, Carlina acaulis extract 9%.

A kind of preparation method of hypoglycemic biscuit as described above, specifically includes following steps：

(1) preparation of functional additive：

Infusion 30-40 minutes are carried out after vine tea to be added to the water of 5-10 times of volume a, are filtered while hot, take filtrate, will The carrying out of filtrate stops heating after heating is concentrated into 1.02 times of original volume, obtains medicinal extract and is subsequently added into cellulase, 70-80% (v/v) ethanol solution carry out surname extraction 1-2 hours, filtrate is dried under reduced pressure after filtering, crush after can obtain vine tea Activity extract, it is stand-by；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 5-8:1:15-20；

B, after 1kg Chinese wistaria seeds are crushed, supercritical CO is put into₂In extraction equipment, 3-4 hours are extracted, collection carries Liquid is taken, filter residue is added to 70-80% (v/v) ethanol solution of 3-5 times of filter residue quality, is carried out refluxing extraction 20-30 minutes, backflow Temperature be at 65-85 DEG C, 0.3 μm of ceramic film component is then poured into 0.1-0.2m/s flow velocity and carries out micro-filtration, in 0.1- Under 0.2MPa pressure differentials, successively with molecular cut off 10000Da, 5000Da membrane module, loop ultrafiltration is carried out with cross-current flow Processing, the Chinese wistaria activity extract that molecular weight ranges are 5000Da-6000Da is obtained, it is stand-by；

C, spina date seed adds 60-80% (v/v) ethanol solution of 3-5 times of powder weight after pulverizing and flowed back Extraction 3 times, 20 minutes every time, water bath method in evaporating dish is placed after filtering, is then dried under reduced pressure to the 1.01 of original volume Times, after adding 70-80% (v/v) ethanol solution progress constant volume containing 2% acetic acid solution, the speed using flow velocity as 0.1-0.2m/s Degree carries out the miillpore filter that aperture is 0.5-0.8 μm and filtered, you can spina date seed activity extract is obtained, it is stand-by；

D, pulp is taken after chaulmoogra fruit is removed the peel, the distilled water that pulp is added to 3-5 times of volume carries out homogeneous mashing, connects Addition solvent to carry out extracting 1-3 hours, then carry out the active material that micro-filtration molecular cut off is 1000-2000Da, will cut Stay liquid to carry out separating 3-5 minutes in the seperator that rotating speed is 2000-3000r/min, take supernatant to be obtained after being spray-dried It is stand-by to chaulmoogra activity extract；

The water that oriental wormwood herb is added to 2-3 times of volume is stirred into slurry e, in mixer, is subsequently added into 3-5 times of water and stirs Uniformly laggard water-filling bath extraction 30-60 minutes are mixed, thick liquid is filtrated to get, thick liquid is poured into polyamide with 0.1-0.2m/s flow velocity Chromatographic column is often chromatographed, and 3 elutions are carried out using 70-80% (v/v) ethanol solution, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.3MPa, elution temperature be 60-70 DEG C, then filtered, be concentrated under reduced pressure, dry, crush after obtain oriental wormwood live Property extract, it is stand-by；

F, broken, the water progress water-bath extraction 20-30 minutes of 5-10 times of volume of addition, extraction after smashing to pieces will be done after garlic peeling Process frequency of use carries out assisted extraction for the microwave of 200-300 hertz, carries out being concentrated under reduced pressure into the 1.02 of original volume after filtering Times, add 20-30% (v/v) methanol solution and carry out 3 elutions, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.3MPa, elution temperature be 60-70 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain garlic activity extract, It is stand-by；

G, the fresh stem of dendrobium candidum is put into mixer and is stirred crushing, then using 65-75% (v/v) ethanol solutions in Temperature is to carry out alcohol extracting at -5--3 DEG C to stay overnight, and uses rotating speed to be centrifuged for 300-400r/min centrifuge after overnight Thick liquid is filtrated to get, normal temperature extracts 1-3 hours after thick liquid is mixed with protease, is filtrated to get dendrobium candidum activity extract, It is stand-by；

H, the water that rice sprout is added to 2-5 times of volume is smashed to pieces, and the high-tension pulse that electric-field intensity is 25KV/cm is used after smashing into slurry to pieces Electric field treatment is rushed, the time of pulse is 5 × 10^-6-10×10^-6S, the frequency of pulse is 30-50 hertz, is added after being handled 5-10 times 70-80% (v/v) ethanol solution carries out ultrasonic extraction 10-20 minutes, the current strength of Ultrasound Instrument using Ultrasound Instrument For 5A/25W-10A/25W, 0.5 μm of ceramic film component is then poured into 0.1-0.25m/s flow velocity and carries out micro-filtration, in 0.1- Under 0.2MPa pressure differentials, successively with molecular cut off 5000000Da, 1O000Da membrane module, it is super that circulation is carried out with cross-current flow Filter is handled, and obtains the spina date seed activity extract that molecular weight ranges are 10000Da-15000Da, stand-by；I, garden burnet stem tuber is entered Row crush after obtain add 70-80% (v/v) ethanol solution carry out circulate extraction obtain extract solution, by extract solution add hydrochloric acid adjust Section pH is 2.0-3.0, is then stirred while heating water bath continues to stir until filiform occur in extract solution and starting timing 5-10 minutes, filtrate is filtered to take in time, filter residue repeats said extracted step 2 time, the filtrate merging of 3 times is dried under reduced pressure, Garden burnet activity extract is can obtain after crushing, it is stand-by；

J, after the flower of globe artichoke is dried, 70-80% (v/v) ethanol solution for adding 5-10 times of volume uses Soxhlet Surname extraction 2-3 hours are carried out after extractor, filter to take the chitosan solution that filtrate adds the 0.01-0.02% of filtrate gross mass Stirring is until filtrate clarification, filters off filtrate and carry out heating concentration, be freeze-dried to obtain globe artichoke activity extract, treat afterwards With；

(2) prepared by biscuit：By black sesame powder, wheat flour, peanut powder low-temperature submicron powder is broken and crosses 200 mesh sieves, and celery is ground Into mud, then the functional additive prepared, egg white, baking powder, maltitol, olive oil, salt solution, citric acid, water are mixed Conjunction stirs into dough, is compacted dough using Action of Gravity Field, is then left to ferment 20-30 minutes, and fermentation temperature is controlled in 35-38℃；Dough is pressed into the uniform musculus cutaneus of 2-4mm thin and thicks with oodle maker, musculus cutaneus is developed and printed into shaping, Ran Houfang with Biscuit mold Enter in baking tray, be placed in electric oven and bakeed, in 170 DEG C of -180 DEG C of baking 10-15min, packed after cooling.

Further instruction, in step a, the activity of the cellulase is the U/g of 10-20 ten thousand.

Further instruction, the Thick many candies content of the fresh stem of dendrobium candidum is 20.56-34.69mg/g.

Further instruction, the garden burnet stem tuber are to pluck the garden burnet stem tuber that is raw and being plucked in 20-25 days in October in 3 years.

Further instruction, the rice sprout are to store the rice sprout after 3-5.

Further instruction, in step g, the addition of protease accounts for the 0.05-0.1% of thick liquid gross mass.

Further instruction, in step (2), the humidity of surrounding environment remains 40-50% during the fermentation.

Vine tea, it is vitaceae ampelopsis Ampelopsis megalophylla Diels et Gilg branch Leaf.Bitter, micro-puckery, it is cool in nature.Clearing heat and promoting diuresis, soothing the liver decompression are promoting blood circulation and removing obstruction in channels.

Chinese wistaria, sweet, bitter, slightly warm in nature, mild toxicity.Return kidney channel.For Shui Yin diseases, edema, arthralgia, intestinal parasitic disease.

Chaulmoogra, acrid flavour, property heat are very toxic.Returns spleen warp, Liver Channel, kidney channel.Dispel pathogenic wind and remove dampness, detoxicating and destroying parasites.Rich in oil, Main component is chaulmoogric acid, hydnocarpic acid etc. in oil.

Oriental wormwood, it is bitter, pungent, it is slightly cold.Returns spleen, stomach, liver, gallbladder channel.Clearing heat and promoting diuresis；There is cholagogic, liver function protecting is antipyretic, resists The effects such as inflammation, reducing blood lipid, decompression, coronary dilatation.

Garlic, warm-natured, acrid flavour.Returns spleen warp, stomach.It is warm-natured, acrid flavour.Returns spleen warp, stomach.It is peppery containing volatile oil about 0.2%, tool Taste and special smell, include the sulfide compound of allicin and a variety of allyls element, propyl group and methyl composition.Gulping down for phagocyte can be improved Function is bitten, strengthens immunocompetence, reduces blood glucose and lipid-metabolism；There is the effect such as anticancer and platelet aggregation-against.

Dendrobium candidum, nourishing kidney-yin, and the fire of deficiency type can drop, and it is sweet, it is slightly cold.Return stomach, kidney channel.For injury thirst, deficiency of food constipation, Abnormal heat is not moved back, and mesh is secretly dim-sighted.

Honeysuckle, it is cold in nature, it is sweet.Return lung warp, the heart channel of Hang-Shaoyin, stomach.Clearing heat and detoxicating, wind-heat dissipating.Containing Luteolin, inositol The chemical composition such as 1% and volatile oil, terpene.Wherein there are chlorogenic acid, isochlorogenic acid, gingko alcohol, β-sitosterol, stigmasterol, beans solid The compositions such as alcohol-D-Glucose glycosides.

Garden burnet, cold nature, sweet, slight bitter.Thoughts of returning home warp, lung channel, stomach.With control dryness of the lung dry cough, haematemesis, lung carbuncle, consumptive disease are tired of Heat, quench one's thirst, pyreticosis Tianjin wound, dry throat, constipation etc..

Rice sprout, it is warm-natured, it is sweet.Returns spleen warp, stomach.With controlling dyspepsia, abdominal distension halitosis, weakness of the spleen and the stomach, not hungry deficiency of food etc..

Globe artichoke, it is mild-natured, sweet；Multiple nutritional components are contained in the edible portion of bouquet, and protein, calcium phosphorus content are higher, in addition Also containing functional compounds such as polyphenol, the flavonoids such as more synanthrin, cynarin, chlorogenic acid.

Spina date seed, it is mild-natured, it is sweet, sour.Return liver warp, gallbladder channel, the heart channel of Hang-Shaoyin.Tonifying liver, calming heart, arrest sweating, promote the production of body fluid.

In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：

1. black sesame powder, wheat flour, peanut powder are with the addition of in the basestocks of the application enriches nutrition principal component, improve biscuit Mouthfeel, rich in there is the health-preserving function that Flavonoid substances, terpene substances can add hypoglycemic biscuit in celery, by addition Functional additive cause rich in there is a substantial amounts of inanimate matter in hypoglycemic biscuit, rich in big in the functional additive of addition The trace element of amount, ferro element participate in the synthesis of hemalbumin, cytochromes and various enzymes, also start shipment oxygen therapy and nutrition in blood The effect of material, the main nutrient composition proportion in the application meets proportion specified in food security, safe and non-toxic, by adding The functional additive added not only have the function that it is hypoglycemic, also have improve biscuit nutritional ingredient, by containing it is micro Flavones, organic acid etc. can support antimicrobial growth, have the function that to extend the shelf life.

2. the health additive added in the application is on the basis of research Traditional Chinese Medicine Coryza Treated by Syndrome Differentiation by the applicant It is upper to refer to modern pharmacological research achievement, by experiment for many years, multiple formulations are screened, have found to type of deficiency of both QI and YIN sugar Urinate the good several drugses of disease therapeutic effect and complete the present invention.In the combination of the additive designed in the present invention, vine tea is clear Hot dampness removing, soothing the liver decompression are promoting blood circulation and removing obstruction in channels；Chinese wistaria is clearing heat and detoxicating；Chaulmoogra dispels pathogenic wind and remove dampness, detoxicating and destroying parasites；Oriental wormwood clearing heat and promoting diuresis；Greatly Garlic row stagnant gas, warming spleen and stomach, disperse accumulations, removing toxic substances desinsection；Dendrobium candidum anti-inflammatory is reduced phlegm and internal heat, nourishing Yin and promoting production of body fluid, is strengthened the spleen and stomach；Honeysuckle heat-clearing solution Poison, wind-heat dissipating；Garden burnet is clearing heat and detoxicating, cooling blood and hemostasis, healing up sore and subduing swelling；Rice sprout promoting digestion and removing indigestion, spleen benefiting and stimulating the appetite；Globe artichoke relieves the effect of alcohol, Protect liver, anti-cancer tumor suppressor；Spina date seed tonifying liver, calming heart, arrest sweating, promote the production of body fluid.10 taste medicine collective effects, complement each other, in 10 taste medicines Containing substantial amounts of active component, the technological means by the application carries out the position active material in extraction Chinese medicine, active material In rich in can largely play a part of the effective active composition and trace element of reduction to blood glucose, trace element can be to human body Function play a part of nutrient environment in balanced body, can preferably carry out reduce blood glucose treatment；Vine tea activity extract In 12.63-38.69mg/g containing vine tea flavone.0.69-2.35mg/g containing diacetone alcohol, rosin spirit in Chinese wistaria activity extract 0.67-2.05mg/g, cupreol 0.68-2.24mg/g, asteriasterol 0.29-0.68mg/g；In chaulmoogra activity extract 2.69-9.26mg/g containing alkaloid, crude fibre 4.56-5.86%.0.68-2.01mg/ containing cumarin in oriental wormwood activity extract G, flavones 1.23-1.96mg/g, organic acid 0.87-1.26mg/g, triterpene 0.79-1.26mg/g, steroidal 0.79-1.36mg/g； 132.05-138.26mg/g containing polysaccharide in dendrobium candidum activity extract, alkaloid 3.65-5.02mg/g, several amino acids and Trace element.Containing saccharomycete, amylase, vitamin, starch, maltose, choline, several amino acids etc. in rice sprout activity extract Composition.1.89-3.15mg/g containing triterpene, saponins material 1.64-2.02mg/g, tannin class material in garden burnet activity extract 1.26-2.35mg/g；1.02-1.68mg/g containing Flavonoid substances, chlorogenic acid 0.56-1.04mg/ in globe artichoke activity extract G, cynarin 0.68-1.63mg/g, Luteolin -7- rutinosides 0.75-0.96mg/g, cynara scolymus glucosides 0.89-1.12mg/g. 0.89-1.12mg/g containing Saponin A, Spine Date Seed jujubosideB 0.89-1.12mg/g, white birch in spina date seed activity extract Resin acid 1.26-2.36mg/g；The interphase interaction of the active material of 10 kinds of raw material extractions, complements each other, compatibility is proper, serves Hypoglycemic biscuit and preparation method thereof of the present invention can not only to diabetic's fasting blood sugar, Area under the curve of blood glucose with It is more suitable than effect with positive drug group and on part index number and cholesterol, the index of triglycerides reduce and be respectively provided with remarkable result Slightly it is better than positive drug group.And use aggregate measures to release the clinical symptoms of diabetic by the method for diagnosis and treatment, prevent The multiple complications of diabetes are controlled, therapeutic effect is notable, can be achieved to treat both principal and secondary aspect of disease, securely and reliably, without any side effects

【Embodiment】

Many details are elaborated in the following description in order to fully understand the present invention.But the present invention can be with Much it is different from other manner described here to implement, those skilled in the art can be in the situation without prejudice to intension of the present invention Under do similar improvement, therefore the present invention is not limited to the specific embodiments disclosed below.

Embodiment 1：

A kind of hypoglycemic biscuit, including basestocks 85% and functional additive 15%；The basestocks are according to weight Percentage includes：Black sesame powder 1%, celery 1%, wheat flour 10%, peanut powder 12%, egg white 8%, baking powder 15%, maltose Alcohol 15%, olive oil 15%, salt solution 7.5%, citric acid 0.5%, water 15%；；The functional additive includes：Vine tea extracts Thing 18%, Chinese wistaria extract 20%, Wild jujube seeds extract 15%, strong wind seed extract 8%, capillary extract 10%, garlic carry Take thing 5%, dendrobium candidum extract 5%, Fructus Setariae Germinatus extract 4%, Radix Sangusorbae extract 5%, Carlina acaulis extract 10%.

(1) preparation of functional additive：

Infusion 30 minutes is carried out after vine tea to be added to the water of 5 times of volumes a, is filtered while hot, takes filtrate, by filtrate Carry out stopping heating after heating is concentrated into 1.02 times of original volume, obtain medicinal extract be subsequently added into activity for 100,000 U/g cellulases, 70% (v/v) ethanol solution carries out surname extraction 1 hour, filtrate is dried under reduced pressure after filtering, crush after can obtain rattan Tea activity extract, it is stand-by；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 8:1:20；

B, after 1kg Chinese wistaria seeds are crushed, supercritical CO is put into₂In extraction equipment, extract 3 hours, collect extraction Liquid, filter residue is added to 70% (v/v) ethanol solution of 3 times of filter residue quality, carry out refluxing extraction 20 minutes, the temperature of backflow is 65 At DEG C, 0.3 μm of ceramic film component is then poured into 0.1m/s flow velocity and carries out micro-filtration, under 0.1MPa pressure differentials, used cut successively Molecular weight 10000Da, 5000Da membrane module are stayed, loop ultrafiltration processing is carried out with cross-current flow, obtaining molecular weight ranges is 6000Da Chinese wistaria activity extract, it is stand-by；

C, spina date seed adds 60% (v/v) ethanol solution progress refluxing extraction 3 of 3 times of powder weight after pulverizing It is secondary, 20 minutes every time, water bath method in evaporating dish is placed after filtering, is then dried under reduced pressure to 1.01 times of original volume, added After entering 70% (v/v) ethanol solution progress constant volume containing 2% acetic acid solution, the speed using flow velocity as 0.1m/s carries out aperture and is 0.5 μm of miillpore filter is filtered, you can spina date seed activity extract is obtained, it is stand-by；

D, pulp is taken after chaulmoogra fruit is removed the peel, the distilled water that pulp is added to 3 times of volumes carries out homogeneous mashing, then Add solvent and carry out extraction 1 hour, the active material that micro-filtration molecular cut off is 1000Da is then carried out, by trapped fluid in rotating speed To carry out separation 3 minutes in 2000r/min seperator, supernatant is taken to can obtain chaulmoogra activity extraction after being spray-dried Thing, it is stand-by；

The water that oriental wormwood herb is added to 2 times of volumes is stirred into slurry e, in mixer, and it is equal to be subsequently added into 3 times of water stirrings Even laggard water-filling bath extraction 30 minutes, is filtrated to get thick liquid, and it is frequent that with 0.1m/s flow velocity thick liquid is poured into polyamide chromatography post Chromatography, 3 elutions are carried out using 70% (v/v) ethanol solution, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.3MPa, The temperature of elution be 60 DEG C, then filtered, be concentrated under reduced pressure, dry, crush after obtain oriental wormwood activity extract, it is stand-by；

It f, will do broken after garlic peeling, the water that 5 times of volumes are added after smashing to pieces carries out water-bath and extracted 20 minutes, and extraction process makes Assisted extraction is carried out with the microwave that frequency is 200 hertz, be concentrated under reduced pressure into 1.02 times of original volume after filtering, adds 20% (v/v) methanol solution carries out 3 elutions, and the pressure of elution is followed successively by -0.1MPa, 0.1MPa, 0.3MPa, the temperature of elution For 60 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain garlic activity extract, it is stand-by；

G, the fresh stem of dendrobium candidum that Thick many candies content is 20.56mg/g is put into mixer and is stirred crushing, then made Alcohol extracting is carried out at temperature is -5 DEG C to stay overnight, centrifugation of the rotating speed for 300r/min is used after overnight with 65% (v/v) ethanol solution Machine, which is centrifuged, is filtrated to get thick liquid, by thick liquid with accounting for normal temperature extraction 1 after 0.05% protease of thick liquid gross mass mixes Hour, dendrobium candidum activity extract is filtrated to get, it is stand-by；

H, the water that the rice sprout after storing 3 years adds 2 times of volumes is smashed to pieces, and it is 25KV/ to smash to pieces into after slurry using electric-field intensity Cm high-pressure pulse electric processing, the time of pulse is 5 × 10^-6S, the frequency of pulse is 30 hertz, and 5 times are added after being handled 70% (v/v) ethanol solution carry out ultrasonic extraction 10 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument is 5A/25W, then 0.5 μm of ceramic film component is poured into 0.1m/s flow velocity and carries out micro-filtration, under 0.1MPa pressure differentials, uses molecular cut off successively 5000000Da, 1O000Da membrane module, loop ultrafiltration processing is carried out with cross-current flow, it is 15000Da to obtain molecular weight ranges Spina date seed activity extract, it is stand-by；

I, it will pluck raw in 3 years and obtain addition 70% (v/v) after the garden burnet stem tuber of harvesting on October 20 is crushed Ethanol solution carries out circulation extraction and obtains extract solution, and it is 2.0 that extract solution is added into salt acid for adjusting pH, is then stirred while water Bath heating continues stirring 5 minutes until occurring filiform in extract solution and starting timing, filters to take filtrate in time, and filter residue repeats above-mentioned Extraction step 2 times, garden burnet activity extract is can obtain after the filtrate merging of 3 times is dried under reduced pressure, crushed, it is stand-by；

J, after the flower of globe artichoke is dried, 70% (v/v) ethanol solution for adding 5 times of volumes uses apparatus,Soxhlet's Surname extraction is carried out afterwards 2 hours, filter to take after filtrate adds 0.01% chitosan solution of filtrate gross mass and stir until filter Liquid is clarified, and is filtered off filtrate and is carried out heating concentration, is freeze-dried to obtain globe artichoke activity extract, stand-by；

(2) prepared by biscuit：By black sesame powder, wheat flour, peanut powder low-temperature submicron powder is broken and crosses 200 mesh sieves, and celery is ground Into mud, then the functional additive prepared, egg white, baking powder, maltitol, olive oil, salt solution, citric acid, water are mixed Conjunction stirs into dough, is compacted dough using Action of Gravity Field, is then left to ferment 20 minutes, and surrounding environment is wet during fermentation Degree remains 40%, fermentation temperature and is controlled in 35 DEG C；Dough is pressed into the uniform musculus cutaneus of 2mm thin and thicks with oodle maker, uses biscuit mould Musculus cutaneus is developed and printed shaping by tool, is then placed in baking tray, is placed in electric oven and is bakeed, and is toasted 15min in 170 DEG C, is wrapped after cooling Dress.

Embodiment 2：

A kind of hypoglycemic biscuit, including basestocks 70% and functional additive 30%；The basestocks are according to weight Percentage includes：Black sesame powder 8%, celery 5%, wheat flour 20%, peanut powder 5%, egg white 15%, baking powder 10%, maltose Alcohol 8%, olive oil 10%, salt solution 8%, citric acid 1.0%, water 10%；The functional additive includes：Ampelopsis grossedentata extrat 10%th, Chinese wistaria extract 10%, Wild jujube seeds extract 8%, strong wind seed extract 15%, capillary extract 5%, garlic P.E 10%th, dendrobium candidum extract 10%, Fructus Setariae Germinatus extract 10%, Radix Sangusorbae extract 12%, Carlina acaulis extract 10%.

(1) preparation of functional additive：

Infusion 40 minutes is carried out after vine tea to be added to the water of 10 times of volumes a, is filtered while hot, takes filtrate, by filtrate Carry out stopping heating after heating is concentrated into 1.02 times of original volume, obtain medicinal extract be subsequently added into activity for 200,000 U/g cellulases, 80% (v/v) ethanol solution carries out surname extraction 2 hours, filtrate is dried under reduced pressure after filtering, crush after can obtain rattan Tea activity extract, it is stand-by；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 5:1:15；

B, after 1kg Chinese wistaria seeds are crushed, supercritical CO is put into₂In extraction equipment, extract 4 hours, collect extraction Liquid, filter residue is added to 80% (v/v) ethanol solution of 5 times of filter residue quality, carry out refluxing extraction 30 minutes, the temperature of backflow is 85 At DEG C, 0.3 μm of ceramic film component is then poured into 0.2m/s flow velocity and carries out micro-filtration, under 0.2MPa pressure differentials, used cut successively Molecular weight 10000Da, 5000Da membrane module are stayed, loop ultrafiltration processing is carried out with cross-current flow, obtaining molecular weight ranges is 5000Da Chinese wistaria activity extract, it is stand-by；

C, spina date seed adds 80% (v/v) ethanol solution progress refluxing extraction 3 of 5 times of powder weight after pulverizing It is secondary, 20 minutes every time, water bath method in evaporating dish is placed after filtering, is then dried under reduced pressure to 1.01 times of original volume, added After entering 80% (v/v) ethanol solution progress constant volume containing 2% acetic acid solution, the speed using flow velocity as 0.2m/s carries out aperture and is 0.8 μm of miillpore filter is filtered, you can spina date seed activity extract is obtained, it is stand-by；

D, pulp is taken after chaulmoogra fruit is removed the peel, the distilled water that pulp is added to 5 times of volumes carries out homogeneous mashing, then Add solvent and carry out extraction 3 hours, the active material that micro-filtration molecular cut off is 2000Da is then carried out, by trapped fluid in rotating speed To carry out separation 5 minutes in 3000r/min seperator, supernatant is taken to can obtain chaulmoogra activity extraction after being spray-dried Thing, it is stand-by；

The water that oriental wormwood herb is added to 3 times of volumes is stirred into slurry e, in mixer, and it is equal to be subsequently added into 5 times of water stirrings Even laggard water-filling bath extraction 60 minutes, is filtrated to get thick liquid, and it is frequent that with 0.2m/s flow velocity thick liquid is poured into polyamide chromatography post Chromatography, 3 elutions are carried out using 80% (v/v) ethanol solution, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.3MPa, The temperature of elution be 70 DEG C, then filtered, be concentrated under reduced pressure, dry, crush after obtain oriental wormwood activity extract, it is stand-by；

F, broken, the water progress water-bath extraction of 10 times of volumes of addition 30 minutes, extraction process after smashing to pieces will be done after garlic peeling The microwave that frequency of use is 300 hertz carries out assisted extraction, be concentrated under reduced pressure into 1.02 times of original volume after filtering, adds Enter 30% (v/v) methanol solution and carry out 3 elutions, the pressure of elution is followed successively by -0.1MPa, 0.1MPa, 0.3MPa, the temperature of elution Spend for 70 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain garlic activity extract, it is stand-by；

G, the fresh stem of dendrobium candidum that Thick many candies content is 34.69mg/g is put into mixer and is stirred crushing, then made Alcohol extracting is carried out at temperature is -3 DEG C to stay overnight, centrifugation of the rotating speed for 400r/min is used after overnight with 75% (v/v) ethanol solution Machine, which is centrifuged, is filtrated to get thick liquid, and thick liquid is small with accounting for normal temperature extraction 3 after 0.1% protease of thick liquid gross mass mixes When, dendrobium candidum activity extract is filtrated to get, it is stand-by；

H, the water that the rice sprout after storing 5 years adds 5 times of volumes is smashed to pieces, and it is 25KV/ to smash to pieces into after slurry using electric-field intensity Cm high-pressure pulse electric processing, the time of pulse is 10 × 10^-6S, the frequency of pulse is 50 hertz, and 10 are added after being handled 80% (v/v) ethanol solution again carries out ultrasonic extraction 20 minutes using Ultrasound Instrument, and the current strength of Ultrasound Instrument is 10A/25W, Then 0.5 μm of ceramic film component is poured into 0.25m/s flow velocity and carries out micro-filtration, under 0.2MPa pressure differentials, successively with retention point Son amount 5000000Da, 1O000Da membrane module, carry out loop ultrafiltration processing, obtaining molecular weight ranges is with cross-current flow 10000Da spina date seed activity extract, it is stand-by；I, it will pluck raw in 3 years and carried out in the garden burnet stem tuber of harvesting on October 25 Obtained after crushing addition 80% (v/v) ethanol solution carry out circulate extraction obtain extract solution, by extract solution add salt acid for adjusting pH For 3.0, then stir while heating water bath continues stirring 10 minutes until occurring filiform in extract solution and starting timing, and When filter to take filtrate, filter residue repeats said extracted step 2 time, the filtrate of 3 times is merged be dried under reduced pressure, crush after can obtain It is stand-by to garden burnet activity extract；

J, after the flower of globe artichoke is dried, 80% (v/v) ethanol solution for adding 10 times of volumes uses surname extraction Surname extraction 3 hours is carried out after device, filter to take filtrate add stirring after 0.02% chitosan solution of filtrate gross mass until Filtrate is clarified, and is filtered off filtrate and is carried out heating concentration, is freeze-dried to obtain globe artichoke activity extract, stand-by；

(2) prepared by biscuit：By black sesame powder, wheat flour, peanut powder low-temperature submicron powder is broken and crosses 200 mesh sieves, and celery is ground Into mud, then the functional additive prepared, egg white, baking powder, maltitol, olive oil, salt solution, citric acid, water are mixed Conjunction stirs into dough, is compacted dough using Action of Gravity Field, is then left to ferment 30 minutes, and surrounding environment is wet during fermentation Degree remains 50%, fermentation temperature and is controlled in 38 DEG C；Dough is pressed into the uniform musculus cutaneus of 4mm thin and thicks with oodle maker, uses biscuit mould Musculus cutaneus is developed and printed shaping by tool, is then placed in baking tray, is placed in electric oven and is bakeed, and is toasted 10min in 180 DEG C, is wrapped after cooling Dress.

Embodiment 3：

A kind of hypoglycemic biscuit, including basestocks 80% and functional additive 20%；The basestocks are according to weight Percentage includes：Black sesame powder 5%, celery 3%, wheat flour 18%, peanut powder 11.2%, egg white 10%, baking powder 12%, malt Sugar alcohol 10%, olive oil 13%, salt solution 5%, citric acid 0.8%, water 12%；；The functional additive includes：Vine tea extracts Thing 14%, Chinese wistaria extract 15%, Wild jujube seeds extract 11%, strong wind seed extract 12%, capillary extract 7%, garlic carry Take thing 6%, dendrobium candidum extract 8%, Fructus Setariae Germinatus extract 8%, Radix Sangusorbae extract 10%, Carlina acaulis extract 9%.

(1) preparation of functional additive：

Infusion 35 minutes is carried out after vine tea to be added to the water of 7 times of volumes a, is filtered while hot, takes filtrate, by filtrate Carry out stopping heating after heating is concentrated into 1.02 times of original volume, obtain medicinal extract be subsequently added into activity for 120,000 U/g cellulases, 75% (v/v) ethanol solution carries out surname extraction 1.5 hours, filtrate is dried under reduced pressure after filtering, crush after can obtain Vine tea activity extract, it is stand-by；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 6:1:17；

B, after 1kg Chinese wistaria seeds are crushed, supercritical CO is put into₂In extraction equipment, extract 3.5 hours, collection carries Liquid is taken, filter residue is added to 75% (v/v) ethanol solution of 4 times of filter residue quality, carries out refluxing extraction 25 minutes, the temperature of backflow is At 70 DEG C, 0.3 μm of ceramic film component is then poured into 0.15m/s flow velocity and carries out micro-filtration, under 0.12MPa pressure differentials, successively With molecular cut off 10000Da, 5000Da membrane module, loop ultrafiltration processing is carried out with cross-current flow, obtains molecular weight ranges It is stand-by for 5500Da Chinese wistaria activity extract；

C, spina date seed adds 70% (v/v) ethanol solution progress refluxing extraction 3 of 4 times of powder weight after pulverizing It is secondary, 20 minutes every time, water bath method in evaporating dish is placed after filtering, is then dried under reduced pressure to 1.01 times of original volume, added After entering 75% (v/v) ethanol solution progress constant volume containing 2% acetic acid solution, the speed using flow velocity as 0.16m/s carries out aperture Miillpore filter for 0.65 μm is filtered, you can spina date seed activity extract is obtained, it is stand-by；

D, pulp is taken after chaulmoogra fruit is removed the peel, the distilled water that pulp is added to 4 times of volumes carries out homogeneous mashing, then Add solvent and carry out extraction 2 hours, the active material that micro-filtration molecular cut off is 1500Da is then carried out, by trapped fluid in rotating speed To carry out separation 4 minutes in 2500r/min seperator, supernatant is taken to can obtain chaulmoogra activity extraction after being spray-dried Thing, it is stand-by；

The water that oriental wormwood herb is added to 3 times of volumes is stirred into slurry e, in mixer, and it is equal to be subsequently added into 4 times of water stirrings Even laggard water-filling bath extraction 40 minutes, is filtrated to get thick liquid, and it is frequent that with 0.15m/s flow velocity thick liquid is poured into polyamide chromatography post Chromatography, 3 elutions are carried out using 74% (v/v) ethanol solution, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.3MPa, The temperature of elution be 65 DEG C, then filtered, be concentrated under reduced pressure, dry, crush after obtain oriental wormwood activity extract, it is stand-by；

It f, will do broken after garlic peeling, the water that 7 times of volumes are added after smashing to pieces carries out water-bath and extracted 25 minutes, and extraction process makes Assisted extraction is carried out with the microwave that frequency is 250 hertz, be concentrated under reduced pressure into 1.02 times of original volume after filtering, adds 25% (v/v) methanol solution carries out 3 elutions, and the pressure of elution is followed successively by -0.1MPa, 0.1MPa, 0.3MPa, the temperature of elution For 65 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain garlic activity extract, it is stand-by；

G, the fresh stem of dendrobium candidum that Thick many candies content is 27.36mg/g is put into mixer and is stirred crushing, then made Alcohol extracting is carried out at temperature is -4 DEG C to stay overnight, centrifugation of the rotating speed for 350r/min is used after overnight with 70% (v/v) ethanol solution Machine, which is centrifuged, is filtrated to get thick liquid, by thick liquid with accounting for normal temperature extraction 2 after 0.08% protease of thick liquid gross mass mixes Hour, dendrobium candidum activity extract is filtrated to get, it is stand-by；

H, the water that the rice sprout after storing 4 years adds 4 times of volumes is smashed to pieces, and it is 25KV/ to smash to pieces into after slurry using electric-field intensity Cm high-pressure pulse electric processing, the time of pulse is 7 × 10^-6S, the frequency of pulse is 40 hertz, and 7 times are added after being handled 75% (v/v) ethanol solution carry out ultrasonic extraction 15 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument is 7A/25W, then 0.5 μm of ceramic film component is poured into 0.2m/s flow velocity and carries out micro-filtration, under 0.12MPa pressure differentials, uses molecular cut off successively 5000000Da, 1O000Da membrane module, loop ultrafiltration processing is carried out with cross-current flow, it is 12000Da to obtain molecular weight ranges Spina date seed activity extract, it is stand-by；I, it will pluck raw in 3 years and be obtained after the garden burnet stem tuber of harvesting on October 22 is crushed Circulation extraction is carried out to 75% (v/v) ethanol solution of addition and obtains extract solution, and it is 2.5 that extract solution is added into salt acid for adjusting pH, is connect Stir while heating water bath until extract solution in occur filiform start timing continue stirring 7 minutes, filter to take in time Filtrate, filter residue repeat said extracted step 2 time, and can obtain garden burnet after the filtrate merging of 3 times is dried under reduced pressure, crushed lives Property extract, it is stand-by；

J, after the flower of globe artichoke is dried, 75% (v/v) ethanol solution for adding 7 times of volumes uses apparatus,Soxhlet's Carry out surname extraction 2.5 hours afterwards, filter to take filtrate add stirring after 0.015% chitosan solution of filtrate gross mass until Filtrate is clarified, and is filtered off filtrate and is carried out heating concentration, is freeze-dried to obtain globe artichoke activity extract, stand-by；

(2) prepared by biscuit：By black sesame powder, wheat flour, peanut powder low-temperature submicron powder is broken and crosses 200 mesh sieves, and celery is ground Into mud, then the functional additive prepared, egg white, baking powder, maltitol, olive oil, salt solution, citric acid, water are mixed Conjunction stirs into dough, is compacted dough using Action of Gravity Field, is then left to ferment 25 minutes, and surrounding environment is wet during fermentation Degree remains 45%, fermentation temperature and is controlled in 37 DEG C；Dough is pressed into the uniform musculus cutaneus of 3mm thin and thicks with oodle maker, uses biscuit mould Musculus cutaneus is developed and printed shaping by tool, is then placed in baking tray, is placed in electric oven and is bakeed, and is toasted 12min in 175 DEG C, is wrapped after cooling Dress.

The health effect test checking of functional additive：

1.1 principle

1-3 of the embodiment of the present invention, comparative example, 0.9% physiological saline (medicine company Group Co., Ltd of Nanning Baihui), four Oxygen pyrimidine (Sigma Co., USA), metformin hydrochloride tablet (Shanghai Shi Guibao pharmaceutical Co. Ltds of Sino-U.S.), blood glucose meter and blood glucose Test paper (Japanese Omron Corp), medical starch (Zhengzhou Hua Yao bio tech ltd).

1.2 build group

Healthy male rat (150 ± 20g) Guangxi University of Chinese Medicine Experimental Animal Center is bought to provide, it is common to maintain material suitable It should raise 5 days, fasting 4 hours, take tail blood, measure i.e. to (0 hour) blood glucose value before glucose, gives 2.5g/kgBW grapes on an empty stomach 0.5,2 hour blood glucose value is determined after sugar, as the batch animal basic value.With 0,0.5 hour blood sugar level point, 16 groups, convert into 0.8g/kg crude drug amounts are administered, and distilled water dissolving, once, each experimental group is seen below daily gastric infusion, every group 10.Blank pair Not processed according to group, 11 experimental group gavages give different given the test agent, and model control group gives same volume solvent, and continuous 35 My god.Each group changes high lipid food after giving the raising of maintenance material 1 week in addition to blank control group, after continuing feeding 3 weeks, except blank control The outer fasting of group 24 hours (can't help water), give STZ 30mg/kg BW intraperitoneal injections, injection volume 1ml/100g body weight.Inject follow-up Continuous high lipid food of giving is fed with 5 days.Off-test, each group animal fasting 4 hours, detection fasting blood-glucose, sugar tolerance, serum islet Element and cholesterol, triglyceride level.

The situation of experimental group is as follows：

1 model control group (high lipid food is fed, and gives same volume solvent gastric infusion, once a day)

1 blank control group (not processing)；

(diabetes pill, dosage 0.5g/kg, the dose,equivalent of rat is equivalent to human body recommended amounts for 1 positive drug control group 5 times, daily equivalent to adult human dose 6g/, once a day)；

1 A Ampelopsis grossedentata extrats control group (equivalent 0.8g/kg crude drug amounts administration, one time a day)；1 B Chinese wistaria extract pair According to group (equivalent 0.8g/kg crude drug amounts administration, one time a day)；1 C Wild jujube seeds extracts control group (equivalent 0.8g/kg crude drug amount Administration, one time a day)；1 D strong wind seed extracts control group (equivalent 0.8g/kg crude drug amounts administration, one time a day)；1 E oriental wormwood Extract control group (equivalent 0.8g/kg crude drug amounts administration, one time a day)；1 F garlic P.Es control group (equivalent 0.8g/kg Crude drug amount is administered, one time a day)；1 G dendrobium candidum extracts control group (equivalent 0.8g/kg crude drug amounts administration, one time a day)；1 Individual H Fructus Setariae Germinatus extracts control group (equivalent 0.8g/kg crude drug amounts administration, one time a day)；1 I Radix Sangusorbae extracts control group is (equivalent 0.8g/kg crude drug amounts are administered, one time a day)；1 J Carlina acaulis extracts control group (converts into 0.8g/kg crude drug amounts to be administered, daily 1 It is secondary)；1 functional additive composition of embodiment 1 (equivalent 0.8g/kg crude drug amounts administration, one time a day)；1 work(of embodiment 2 Can property compositions of additives (equivalent 0.8g/kg crude drug amounts administration, one time a day)；1 functional additive composition of embodiment 3 (equivalent 0.8g/kg crude drug amounts administration, one time a day).

2.1 observation index

2.1.1 fasting blood-glucose, sugar tolerance

Each group animal fasting 4 hours, measure fasting blood-glucose are given not to (0 hour) blood glucose value, dosage group before glucose With concentration of test sample, model control group gives same volume solvent, and blank control group does not process, and each group is orally given after 20 minutes Glucose 2.5g/kg BW are given, determines and gives each group after glucose the blood glucose value of 0.5,2 hour, if 0.5 hour blood of model control group Sugared value >=10mmol/L, or model control group 0.5 hour, the point blood glucose rise of 2 hour any time or Area under the curve of blood glucose liter Height, compared with blank control group, difference has conspicuousness, and decision model carbohydrate metabolism disturbance is set up, on this basis, observing and nursing pair According to group with test sample group fasting blood-glucose, to (0.5,2 hour) blood glucose after glucose and 0,0.5,2 hour Area under the curve of blood glucose Change.

Blood glucose value × 100% before blood glucose rate of descent %=(blood glucose value after blood glucose value-experiment before experiment)/experiment

Area under the curve of blood glucose==1/2 × (+0.5 hour blood glucose of 0 hour blood glucose) × 0.5+1/2 × (0.5 hour blood glucose + 1 hour blood glucose) × 0.5+1/2 × (+2 hours blood glucose of 1 hour blood glucose) × 1+1/2 × (+3 hours blood glucose of 2 hours blood glucose) × 1

2.1.2 cholesterol, triglyceride

Each group animal fasting 4 hours, detection serum cholesterol, triglyceride, if model control group serum cholesterol or sweet Oily three esters are significantly raised, and compared with blank control group, difference has conspicuousness, and decision model disorders of lipid metabolism is set up, basic herein On, observing and nursing control group and test sample group Blood Lipid.

2.1.3 insulin

Each group animal fasting 4 hours, detect serum insulin, model control group insulin resistance compared with blank control group Index is without being decreased obviously, and animal sugar/disorders of lipid metabolism is set up, judge insulin resistance sugar hyperlipoidemia success.See Examine model control group and test sample group insulin resistance condition.

Insulin resistance index=insulin/22.5e^{- ㏑ blood glucose}≈ (blood glucose × insulin)/22.5

2.1.4 data processing and result judgement

Using variance analysis, but homogeneity test of variance need to be first carried out by the program of variance analysis, variance is neat, calculates F values, F Value<F 0.05, conclusion：No significant difference between each group mean；F values >=F0.05, P≤0.05 are right with multiple experimental groups and one Counted according to the comparative approach two-by-two of mean between group.

3.1 data processings and result judgement

Raising 5 days, the fasting blood sugar of fasting 4 hours is shown in Table 1：

Table 1

Significance analysis：*：With model group than * P<0.05, * * P<0.01；#：Compare #P with positive drug group<0.05, ##P< 0.01.The testing result table of resistance to sugar amount is shown in Table 2：

Table 2

Significance analysis：*:With model than * P<0.05, * * P<0.01；#：Compare #P with positive drug group<0.05, ##P<0.01.

T-CHOL, triglyceride determination result hand over table 3

Table 3

It is can be seen that by table 1-3 by measurement results such as fasting blood sugar, sugar tolerance, cholesterol, triglycerides.Fasting blood-glucose In value, the high low dose group of composition has significant difference with model group ratio.Had very using the functional additive of the application Good hypoglycemic effect, the embodiment that compares 1 and A-J groups, single addition Ampelopsis grossedentata extrat, Chinese wistaria extract, spina date seed carry Take thing, strong wind seed extract, capillary extract, garlic P.E, dendrobium candidum extract, Fructus Setariae Germinatus extract, Radix Sangusorbae extract Or Carlina acaulis extract is arrived the significant effect of embodiment 1 in hypoglycemic.

Functional additive is added during the biscuit of fermentation blood sugar-reducing in the present invention and carries out raw material, 10 taste medicines are carried Shared after taking active portion, can effectively reach reduction blood glucose, prevention and the effect for improving diabetic complication.

4.1 toxicological test：

Acute toxicity test：Mouse, rat limosis gavage product of the present invention, observe 7 days, male and female mouse and rat are showed no Poisoning symptom, no death, product male and female mouse of the present invention and rat peroral acute toxicity experiment MTD>It is 10.0g/kg.BW true The nontoxic level in border.

With the medicament of E1 groups in the application to mouse, rat limosis gavage, thing is dead after observation 14 days, to male and female mouse and Rat is showed no poisoning symptom, no death, and animal subject is not put to death and carries out dissection inspection by the observation period, liver, board, kidney, stomach, intestines, The main organs such as the heart, lung, show no obvious abnormalities change.Product male and female mouse of the present invention and rat peroral acute toxicity experiment MTD> 10.0g/kg.BW according to《Health food is examined and assessment technique specification)) acute toxicity grading criteria in (version in 2003), Belong to nontoxic level.

Product Salmonella reversion test of the present invention, mouse marrow cell micro nuclear test and mouse sperm distortion test result are the moon Property, prompt product safety of the present invention.

During 30 days feeding trials of product of the present invention, the activity of animal, ingest, the ordinary circumstance such as defecation has no obvious different Often, and the body weight to animal, food-intake, routine blood indexes, organ weights have no significant effect, and are in female male rat high dose group Items Testing index does not observe anomalous variation during 20.00g/kg.BW (equivalent to 500 times of human body recommended amounts).

5.1 clinical test

Present invention treatment deficiency of both qi and yin disease is quenched one's thirst patient the effect of carry out clinical observation, pass through and contrast that medication is front and rear to suffer from Person uses the amount analysis of insulin hypoglycemic effect of the invention.

5.1.1 inclusive criteria

It is all have it is thirsty drink, the paddy that disappears is easily hungry, urine is more and sweet tea, body are gradually shown in that patient of becoming thin can be diagnosed as diabete (diabetes)；All lassitude hypodynamias, the lazy speech of spontaneous sweating, shortness of breath, thirst and liking drink, dysphoria in chestpalms-soles, palpitation and insomnia, urinate red constipation, tongue HongShaoJin(SIC), tongue body fat big, tongue are thin or flower is shelled, small and wiry pulse or number person, as Qi and Yin deficiency diabetes mellitus；There is diabetic symptom, appoint When between blood glucose ＞ 11.1mmol/L or fasting blood-glucose ＞ 7.0mmol/L；Diabetes B is had been determined as, long-term use of diformazan is double The patient of guanidine or the patient of long term injections insulin；All patients meet 1 or more than 1 in above-mentioned 4.

5.1.2 case selection

It is all while meet above-mentioned doctor trained in Western medicine criteria for classification, TCM syndrome diagnostic criteria patient；18-65 year person of age；It is ready to connect By 2 weeks curers；Signature enters clinical research informed consent form person.

5.1.3 medicine

Experimental group 1：By the functional additive 10g in embodiment 1, it is dissolved in 100ml water and obtains test solvent a；Experiment Group 2：By the functional additive 10g in embodiment 1, it is dissolved in 100ml water and obtains test solvent b；Experimental group 3：By embodiment 1 In functional additive 10g, be dissolved in 100ml water and obtain test solvent c；Regular iletin：The biochemical medicine of the nation of Jiangsu ten thousand Limited company, lot number：1007217.

5.1.4 clinical test is grouped

Comparative example group：Insulin；Test example group：Test solvent a+ insulin.

5.1.5 observation index：The situation of change of main clinic symptoms and sign is observed, on an empty stomach and postprandial 2 hours blood glucose is examined Survey；Pancreas islet

The decrement of plain dosage.

5.1.6 treatment method：

5.1.6.1 start before administration must continuous 3 inspections blood glucose mean be fasting blood-glucose ＞ 7.0mmol/L, postprandial blood Sugar is ＞ 11.1mmol/L；Other any drops must be disabled before meeting 200 clothes for patients development clinical test of inclusion criteria The Chinese and Western medicine of sugar.

5.1.6.2 50 patients of comparative example group continue to use insulin；1 group of 50 clothes for patients test solvent a is tested, often Secondary 20ml, 3 times a day, 30 minutes before meals oral, and 2 weeks are 1 course for the treatment of, tests 2 groups of 50 clothes for patients test solvent b, every time 20ml, 3 times a day, 30 minutes before meals oral, and 2 weeks are 1 course for the treatment of, tests 3 groups of 50 clothes for patients test solvent c, every time 20ml, 3 times a day, 30 minutes before meals oral, and 2 weeks are 1 course for the treatment of, and therapeutic process controls blood glucose in standard with the use of insulin In the range of.

5.1.6.3 therapeutic process strictly carries out dietetic treatment.

5.1.7 result：Test group patient is gradually reduced in 2 weeks or so blood glucose of taking medicine, and insulin usage amount gradually reduces, drop Sugar effect is steady, and the observation to safety indexes shows, medicine of the present invention has no adverse reaction.Insulin dosage is shown in after clinical treatment Following table.

Insulin dosage (x ± s) (n=100) after table 4 is treated

Group	Insulin dosage
		Contrast groups	50±3.5U
Experimental group 1	28±2.4U
		Experimental group 2	29±3.9U
Experimental group 3	25±2.9U

Test example group is compared with comparative example group, P<0.01.

As a result show：With the use of the dosage of insulin while treatment using the present invention, controlled with insulin is used alone Dosage during treatment is compared, and has pole significant difference (P<0.01), illustrate that Chinese medicine composition of the present invention is suffered to type of deficiency of both QI and YIN diabetes Person has notable hypoglycemic effect, and its insulin dosage is substantially reduced to insulin-dependent patient.

And the patient to taking test solvent a, b, c respectively carries out two courses for the treatment of and takes rear physical examination, result of the test shows, clothes After two courses for the treatment of, type of deficiency of both QI and YIN diabetes patient clinical symptom can be obviously improved, reduces fasting blood-glucose and 2h-plasma glucose (P<0.05)；Blood, urine, stool and hepatic and renal function items Testing index be in normal range (NR) before and after test-meal, prompt test solvent a, B, c medicine has treatment diabetes effect to tested patients' health without obvious infringement.

2nd, the Quality Detection of hypoglycemic biscuit：

Sample to be detected is：

Embodiment 1, embodiment 2, embodiment 3, control group：The breakfast biscuits for a certain brand purchased on the market.

The project of detection is as follows：

Determination of moisture uses GB/T5009.3-2003；Reducing sugar test uses Fehling reagent colorimetric method；Content of starch determines Using GB/5009.9-2008 acid-hydrolysis methods；Ash determination uses the direct ashing methods of GB/5009.4-2003；Protein content is surveyed Surely Kjeldahl's method is used；Fat test uses GB/T5009.6-2003 soxhlet extraction methods；Crude fibre measure uses GB/ T5009.10-2003；Flavones measure uses optics photometry.

The comparison of ingredients table of table 5

As seen from the above table, using the application technique compared with the hypoglycemic biscuit that raw material is prepared contrast groups, fat The low matters of fat content give with hyperglycaemia patient use, and additional functional additive change to a certain degree biscuit into Point, coarse-fibred amount improves compared with control group as the table shows so that biscuit is more crisp soft；By extraction process system The micro- composition in biscuit can be improved rich in substantial amounts of flavones, tannin, organic acid in standby functional additive, is compared It is apparent that flavonoids, the control group that compares improves 0.003g/100g, ash content, reduced sugar, content of starch proportion hardly Become, meet the regulation of biscuit nutritional ingredient proportion in food.

Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art, Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.

Claims

A kind of 1. hypoglycemic biscuit, it is characterised in that：Including basestocks 70-85% and functional additive 15-30%；It is described Basestocks include according to percetage by weight：Black sesame powder 1-8%, celery 1-5%, wheat flour 10-20%, peanut powder 5-12%, egg Clear 8-15%, baking powder 10-15%, maltitol 8-15%, olive oil 10-15%, salt solution 5-8%, citric acid 0.5- 1.0%th, water 10-15%；The functional additive includes：Ampelopsis grossedentata extrat 10-18%, Chinese wistaria extract 10-20%, wild jujube Benevolence extract 8-15%, strong wind seed extract 8-15%, capillary extract 5-10%, garlic P.E 5-10%, dendrobium candidum Extract 5-10%, Fructus Setariae Germinatus extract 3-10%, Radix Sangusorbae extract 5-12%, Carlina acaulis extract 5-10%.
A kind of 2. hypoglycemic biscuit as claimed in claim 1, it is characterised in that：The basestocks are according to percetage by weight bag Include：Black sesame powder 5%, celery 3%, wheat flour 18%, peanut powder 11.2%, egg white 10%, baking powder 12%, maltitol 10%th, olive oil 13%, salt solution 5%, citric acid 0.8%, water 12%；The functional additive includes：Ampelopsis grossedentata extrat 14%th, Chinese wistaria extract 15%, Wild jujube seeds extract 11%, strong wind seed extract 12%, capillary extract 7%, garlic extraction Thing 6%, dendrobium candidum extract 8%, Fructus Setariae Germinatus extract 8%, Radix Sangusorbae extract 10%, Carlina acaulis extract 9%.
A kind of 3. preparation method of hypoglycemic biscuit as described in claim 1-2 any one, it is characterised in that：Specifically include Following steps：

(1) preparation of functional additive：

Infusion 30-40 minutes are carried out after vine tea to be added to the water of 5-10 times of volume a, are filtered while hot, take filtrate, by filtrate Carry out stop heating after heating is concentrated into 1.02 times of original volume, obtain medicinal extract and be subsequently added into cellulase, 70-80% (v/ V) ethanol solution carries out surname extraction 1-2 hours, filtrate is dried under reduced pressure after filtering, crush after to can obtain vine tea active Extract, it is stand-by；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 5-8:1:15-20；

B, after 1kg Chinese wistaria seeds are crushed, supercritical CO is put into₂In extraction equipment, 3-4 hours are extracted, collect extract solution, Filter residue is added to 70-80% (v/v) ethanol solution of 3-5 times of filter residue quality, carries out refluxing extraction 20-30 minutes, the temperature of backflow Spend at 65-85 DEG C, then to pour into 0.3 μm of ceramic film component with 0.1-0.2m/s flow velocity and carrying out micro-filtration, in 0.1-0.2MPa Under pressure differential, successively with molecular cut off 10000Da, 5000Da membrane module, loop ultrafiltration processing is carried out with cross-current flow, obtained It is stand-by to the Chinese wistaria activity extract that molecular weight ranges are 5000Da-6000Da；

C, spina date seed adds 60-80% (v/v) ethanol solution progress refluxing extraction of 3-5 times of powder weight after pulverizing 3 times, 20 minutes every time, water bath method in evaporating dish is placed after filtering, is then dried under reduced pressure to 1.01 times of original volume, added After entering 70-80% (v/v) ethanol solution progress constant volume containing 2% acetic acid solution, the speed using flow velocity as 0.1-0.2m/s is entered Row aperture is that 0.5-0.8 μm of miillpore filter is filtered, you can spina date seed activity extract is obtained, it is stand-by；

D, pulp is taken after chaulmoogra fruit is removed the peel, the distilled water that pulp is added to 3-5 times of volume carries out homogeneous mashing, then adds Enter solvent to carry out extracting 1-3 hours, the active material that micro-filtration molecular cut off is 1000-2000Da is then carried out, by trapped fluid Carry out separating 3-5 minutes in the seperator that rotating speed is 2000-3000r/min, take supernatant to can obtain after being spray-dried big Wind activity extract, it is stand-by；

The water that oriental wormwood herb is added to 2-3 times of volume is stirred into slurry e, in mixer, and it is equal to be subsequently added into 3-5 times of water stirring Even laggard water-filling bath extraction 30-60 minutes, thick liquid is filtrated to get, thick liquid is poured into polyamide chromatography with 0.1-0.2m/s flow velocity Post is often chromatographed, and 3 elutions are carried out using 70-80% (v/v) ethanol solution, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.3MPa, elution temperature be 60-70 DEG C, then filtered, be concentrated under reduced pressure, dry, crush after obtain oriental wormwood live Property extract, it is stand-by；

F, broken, the water progress water-bath extraction 20-30 minutes of 5-10 times of volume of addition, extraction process after smashing to pieces will be done after garlic peeling Frequency of use carries out assisted extraction for the microwave of 200-300 hertz, carries out being concentrated under reduced pressure into 1.02 times of original volume after filtering i.e. Can, add 20-30% (v/v) methanol solution and carry out 3 elutions, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.3MPa, The temperature of elution be 60-70 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain garlic activity extract, it is stand-by；

G, the fresh stem of dendrobium candidum is put into mixer and is stirred crushing, then using 65-75% (v/v) ethanol solution in temperature Stayed overnight to carry out alcohol extracting at -5--3 DEG C, use rotating speed that filtering is centrifuged for 300-400r/min centrifuge after overnight Thick liquid is obtained, normal temperature extracts 1-3 hours after thick liquid is mixed with protease, is filtrated to get dendrobium candidum activity extract, stand-by；

H, the water that rice sprout is added to 2-5 times of volume is smashed to pieces, and the high-voltage pulse electric that electric-field intensity is 25KV/cm is used after smashing into slurry to pieces Field processing, the time of pulse is 5 × 10^-6-10×10^-6S, the frequency of pulse is 30-50 hertz, and 5-10 is added after being handled 70-80% (v/v) ethanol solution again carries out ultrasonic extraction 10-20 minutes using Ultrasound Instrument, and the current strength of Ultrasound Instrument is 5A/ 25W-10A/25W, 0.5 μm of ceramic film component is then poured into 0.1-0.25m/s flow velocity and carries out micro-filtration, is pressed in 0.1-0.2MPa Under power difference, successively with molecular cut off 5000000Da, 1O000Da membrane module, loop ultrafiltration processing is carried out with cross-current flow, The spina date seed activity extract that molecular weight ranges are 10000Da-15000Da is obtained, it is stand-by；I, after garden burnet stem tuber is crushed Obtain add 70-80% (v/v) ethanol solution carry out circulate extraction obtain extract solution, by extract solution addition salt acid for adjusting pH be 2.0-3.0, then stir while heating water bath continues to stir 5-10 points until occurring filiform in extract solution and starting timing Clock, filtrate is filtered to take in time, filter residue repeats said extracted step 2 time, after the filtrate merging of 3 times is dried under reduced pressure, crushed Garden burnet activity extract is can obtain, it is stand-by；

J, after the flower of globe artichoke is dried, 70-80% (v/v) ethanol solution for adding 5-10 times of volume uses surname extraction Surname extraction 2-3 hours are carried out after device, are stirred after filtering to take the chitosan solution for the 0.01-0.02% that filtrate adds filtrate gross mass Mix up to filtrate clarification, filter off filtrate and carry out heating concentration, be freeze-dried to obtain globe artichoke activity extract, it is stand-by；

(2) prepared by biscuit：By black sesame powder, wheat flour, peanut powder low-temperature submicron powder is broken and crosses 200 mesh sieves, and celery is ground into mud, Then the functional additive prepared, egg white, baking powder, maltitol, olive oil, salt solution, citric acid, water are mixed Into dough, dough is compacted using Action of Gravity Field, is then left to ferment 20-30 minutes, fermentation temperature is controlled in 35-38 ℃；Dough is pressed into the uniform musculus cutaneus of 2-4mm thin and thicks with oodle maker, musculus cutaneus is developed and printed into shaping with Biscuit mold, is then placed in roasting In disk, it is placed in electric oven and is bakeed, in 170 DEG C of -180 DEG C of baking 10-15min, is packed after cooling.
A kind of 4. preparation method of hypoglycemic biscuit according to claim 3, it is characterised in that：In step a, the fibre The activity for tieing up plain enzyme is the U/g of 10-20 ten thousand.
A kind of 5. preparation method of hypoglycemic biscuit according to claim 3, it is characterised in that：The dendrobium candidum is fresh The Thick many candies content of stem is 20.56-34.69mg/g.
A kind of 6. preparation method of hypoglycemic biscuit according to claim 3, it is characterised in that：The garden burnet stem tuber is Pluck the garden burnet stem tuber that is raw and being plucked in 20-25 days in October in 3 years.
A kind of 7. preparation method of hypoglycemic biscuit according to claim 3, it is characterised in that：The rice sprout is storage Rice sprout after 3-5.
A kind of 8. preparation method of hypoglycemic biscuit according to claim 3, it is characterised in that：In step g, protease Addition account for the 0.05-0.1% of thick liquid gross mass.
A kind of 9. preparation method of hypoglycemic biscuit according to claim 3, it is characterised in that：It is described in step (2) The humidity of surrounding environment remains 40-50% during fermentation.