CN107802027A - A kind of method that pure tobacco oil is prepared using biology enzyme - Google Patents

A kind of method that pure tobacco oil is prepared using biology enzyme Download PDF

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Publication number
CN107802027A
CN107802027A CN201710906352.7A CN201710906352A CN107802027A CN 107802027 A CN107802027 A CN 107802027A CN 201710906352 A CN201710906352 A CN 201710906352A CN 107802027 A CN107802027 A CN 107802027A
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CN
China
Prior art keywords
tobacco
oil
petroleum ether
enzymolysis
extract
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Pending
Application number
CN201710906352.7A
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Chinese (zh)
Inventor
陈建华
刘迎春
黄春兴
禤建国
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Kunming Kun Nine Flavors & Fragrances Co Ltd
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Kunming Kun Nine Flavors & Fragrances Co Ltd
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Priority to CN201710906352.7A priority Critical patent/CN107802027A/en
Publication of CN107802027A publication Critical patent/CN107802027A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/20—Biochemical treatment
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
    • A24B15/18—Treatment of tobacco products or tobacco substitutes
    • A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
    • A24B15/26—Use of organic solvents for extraction

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  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Manufacture Of Tobacco Products (AREA)

Abstract

A kind of method that pure tobacco oil is prepared using biology enzyme, after handling tobacco material using complex enzyme formulation, through alcoholic extraction, the method for petroleum ether back dissolving prepares absolute oil, and wherein complex enzyme formulation vigor proportioning is：The 350U/ml of pectase 250, the 1000U/ml of cellulase 800, the 600U/ml of hemicellulase 500, the 1000U/ml of alpha amylase 800, the 1000U/ml of beta amylase 800, the 100U/ml of lipase 50.Gained absolute oil product fragrance is sent out, is plentiful thoroughly, and natural tobacco perfume (or spice) is purer, more adapts to current tobacco demand.

Description

A kind of method that pure tobacco oil is prepared using biology enzyme
Technical field
The present invention relates to a kind of tobacco aromaticss preparation method, more particularly to one kind using a variety of cellulase treatment offals, The method that tobacco leaf prepares pure tobacco oil.
Technical background
Pure tobacco oil is raw material irreplaceable in perfuming cigarette, non-burning tobacco and part cigarette auxiliary materials, and it forms multiple Miscellaneous, fragrance is plentiful, pure, and pleasant impression is comfortable.Pure tobacco oil prepared by different process has respective feature and purposes, but common process This fragrant difference of the absolute oil fragrance and tobacco of extraction is big, and volatilization or half volatile ingredient are relatively low, and aromatic style is relatively single, it is impossible to complete Current tobacco Related product exploitation demand is adapted to entirely.
The content of the invention
Present invention aims at providing one kind to utilize biology enzyme, the method for improving pure tobacco oil extraction process, make full use of Tobacco material, improve tobacco extract small molecular aroma component content, make fragrance it is more plentiful, closer into this perfume of cured tobacco, Improve tobacco extract availability.
The present invention is achieved through the following technical solutions：
A kind of method that pure tobacco oil is prepared using biology enzyme, using following steps：
1) offal or tobacco leaf are crushed to 20-80 mesh by sterilizing, are added suitable quantity of water, are stirred and evenly mixed, at 80 DEG C of -100 DEG C of sterilizings Reason；40 DEG C of -50 DEG C of insulations；
2) enzymolysis adds complex enzyme formulation enzymolysis 1-3 hours, and regulation PH4-7 is sufficiently stirred；85 degree of alcohol circumfluence distillations Tobacco powder after enzymolysis；Extract petroleum ether extraction, it is concentrated to give absolute oil product；Wherein, enzyme activity proportioning is：
Pectase 250-350U/ml；
Cellulase 800-1000U/ml；
Hemicellulase 500-600U/ml；
Alpha-amylase 800-1000U/ml；
Beta amylase 800-1000U/ml；
Lipase 50-100U/ml；
3), enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, It is 85 ° to adjust alcoholic strength, circumfluence distillation 1.5 hours, after cooling, filtering, concentration filtrate, obtains tobacco extract；
4), back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, mistake Filter；After residue desolventizing insoluble in petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, precipitation Required pure tobacco oil is obtained after agent.
By enzyme-added processing, obtained pure tobacco oil and tobacco obtained by same materials traditional extraction technique are net for offal or tobacco leaf Oil phase ratio, fragrance are sent out, are plentiful thoroughly, and natural tobacco perfume (or spice) is purer, there is more preferable availability.In perfuming cigarette, dripping pill core, electronics Application on cigarette has certain advantage compared with absolute oil prepared by traditional handicraft.It is shown in Table 1.
Table 1 applies sense organ evaluating meter to improve absolute oil with compareing absolute oil
Table 1
Note：Different tests target organoleptic smokes panel test comprehensive grading because evaluation criterion difference does not have comparativity.
Embodiment
Embodiment one
1st, sterilize：1kg offal or tobacco leaf is weighed, is crushed to 20-80 mesh, adds 500g deionized waters, 100 revs/min are stirred Mixing is mixed, 85 DEG C of constant temperature is heated rapidly to and sterilizes for 10 minutes, system is quickly cooled to 45 DEG C of perseverances by sterilization time after with cold water Temperature.
2nd, digest：Tobacco powder gross weight 0.1-0.2%% (W/W) complex enzyme formulation is added, PH is adjusted with hydrochloric acid and sodium hydroxide For 5.5, it is sufficiently stirred, enzymolysis time is 1 hour, and complex enzyme formulation enzyme activity proportioning is as follows：
Pectase 250-350U/ml
Cellulase 800-1000U/ml
Hemicellulase 500-600U/ml
Alpha-amylase 800-1000U/ml
Beta amylase 800-1000U/ml
Lipase 50-100U/ml
3rd, enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, regulation Alcoholic strength, circumfluence distillation 1.5 hours, cooling, filtering, after concentrating filtrate, obtain tobacco extract.
4th, back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, filter. After residue desolventizing insoluble in petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, after desolventizing Obtain required pure tobacco oil.
Embodiment two
1st, sterilize：1kg offal or tobacco leaf is weighed, is crushed to 20-80 mesh, adds 500g deionized waters, 100 revs/min are stirred Mixing is mixed, 85 DEG C of constant temperature is heated rapidly to and sterilizes for 10 minutes, system is quickly cooled to 45 DEG C of perseverances by sterilization time after with cold water Temperature.
2nd, digest：Tobacco powder gross weight 0.1-0.2%% (W/W) complex enzyme formulation is added, PH is adjusted with hydrochloric acid and sodium hydroxide For 5.5, it is sufficiently stirred, enzymolysis time is 2 hours, and complex enzyme formulation enzyme activity proportioning is as follows：
Pectase 250-350U/ml
Cellulase 800-1000U/ml
Hemicellulase 500-600U/ml
Alpha-amylase 800-1000U/ml
Beta amylase 800-1000U/ml
Lipase 50-100U/ml
3rd, enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, regulation Alcoholic strength, circumfluence distillation 1.5 hours, cooling, filtering, after concentrating filtrate, obtain tobacco extract.
4th, back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, filter. After residue desolventizing insoluble in petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, after desolventizing Obtain required pure tobacco oil.
Embodiment three
1st, sterilize：1kg offal or tobacco leaf is weighed, is crushed to 20-80 mesh, adds 500g deionized waters, 100 revs/min are stirred Mixing is mixed, 85 DEG C of constant temperature is heated rapidly to and sterilizes for 10 minutes, system is quickly cooled to 45 DEG C of perseverances by sterilization time after with cold water Temperature.
2nd, digest：Tobacco powder gross weight 0.1-0.2%% (W/W) complex enzyme formulation is added, PH is adjusted with hydrochloric acid and sodium hydroxide For 5.5, it is sufficiently stirred, enzymolysis time is 3 hours, and complex enzyme formulation enzyme activity proportioning is as follows：
Pectase 250-350U/ml
Cellulase 800-1000U/ml
Hemicellulase 500-600U/ml
Alpha-amylase 800-1000U/ml
Beta amylase 800-1000U/ml
Lipase 50-100U/ml
3rd, enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, regulation Alcoholic strength, circumfluence distillation 1.5 hours, cooling, filtering, after concentrating filtrate, obtain tobacco extract.
4th, back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, filter. After residue desolventizing insoluble in petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, after desolventizing Obtain required pure tobacco oil.

Claims (1)

  1. A kind of 1. method that pure tobacco oil is prepared using biology enzyme, it is characterised in that：Using following steps：
    1), offal or tobacco leaf are crushed to 20-80 mesh by sterilizing, are added suitable quantity of water, are stirred and evenly mixed, in 80 DEG C of -100 DEG C of sterilization treatments； 40 DEG C of -50 DEG C of insulations；
    2), enzymolysis adds complex enzyme formulation enzymolysis 1-3 hours, and regulation PH 4-7 are sufficiently stirred；85 degree of alcohol circumfluence distillation enzymes Tobacco powder after solution；Extract petroleum ether extraction, it is concentrated to give absolute oil product；Wherein, enzyme activity proportioning is：
    Pectase 250-350U/ml；
    Cellulase 800-1000U/ml；
    Hemicellulase 500-600U/ml；
    Alpha-amylase 800-1000U/ml；
    Beta amylase 800-1000U/ml；
    Lipase 50-100U/ml；
    3), enzyme deactivation and extraction：Tobacco powder after enzymolysis is transferred to extractor, add 10 times of weight than 95% edible alcohol, regulation Alcoholic strength is 85 °, circumfluence distillation 1.5 hours, after cooling, filtering, concentration filtrate, obtains tobacco extract；
    4), back dissolving extracts：By tobacco extract obtained above with 5 times of weight than petroleum ether fully dissolve, clarify, filter；No After being dissolved in the residue desolventizing of petroleum ether, a tobacco extract byproduct is obtained；Inspissated oil ether dissolves part, is obtained after desolventizing To required pure tobacco oil.
CN201710906352.7A 2017-09-29 2017-09-29 A kind of method that pure tobacco oil is prepared using biology enzyme Pending CN107802027A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109007955A (en) * 2018-06-21 2018-12-18 重庆中烟工业有限责任公司 A kind of cabo extractive, preparation method and tobacco product
CN109280581A (en) * 2018-09-20 2019-01-29 云南中烟工业有限责任公司 A kind of high efficiency preparation method and purposes of pure tobacco oil
CN112471586A (en) * 2020-11-20 2021-03-12 河北中烟工业有限责任公司 Method for simultaneously preparing tobacco essential oil and tobacco juice
CN115088860A (en) * 2022-07-05 2022-09-23 贵州黄果树金叶科技有限公司 Method for preparing tobacco absolute oil by treating tobacco waste through enzymolysis, tobacco absolute oil and application

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JPH0568526A (en) * 1991-09-12 1993-03-23 T Hasegawa Co Ltd Preparation of extract for tobacco flavoring
CN1400300A (en) * 2001-08-01 2003-03-05 云南瑞升科技有限公司 Method for deep-processing tobacco extract to prepare tobacco cleaned oil and enriched solanesol
CN1732812A (en) * 2005-09-12 2006-02-15 华宝食用香精香料（上海）有限公司 Method for improving tobacco extractive incense by multiple biological enzymes
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CN101284766A (en) * 2008-06-04 2008-10-15 云南瑞升烟草技术（集团）有限公司 Process for finely purifying high-purity solanesol form coarse cream of solanesol
CN102178346A (en) * 2011-05-30 2011-09-14 昌宁德康生物科技有限公司深圳分公司 Production method for extracting absolute oil of tobacco leaves
EP1668113B1 (en) * 2003-08-11 2013-06-19 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
CN103627525A (en) * 2013-09-11 2014-03-12 上海聚华科技股份有限公司 Method for preparing tobacco essential oil by reduced pressure distillation
CN104872814A (en) * 2015-05-05 2015-09-02 昌宁德康生物科技有限公司 Production method for extracting tobacco leaf clean oil
CN105105321A (en) * 2015-10-14 2015-12-02 中国烟草总公司郑州烟草研究院 Electronic cigarette tobacco extract without sweet greasy taste and electronic liquid
CN105256654A (en) * 2014-06-09 2016-01-20 云南红塔蓝鹰纸业有限公司 Cigarette paper having cigarette sucking taste improving function, and preparation method thereof
CN106047491A (en) * 2016-05-09 2016-10-26 南京泽朗医药科技有限公司 An extracting method for oregano volatile oil
CN106221935A (en) * 2016-08-26 2016-12-14 云南养瑞科技集团有限公司 The preparation method of a kind of Pericarpium Citri Reticulatae absolute oil and the application in Medicated cigarette thereof
CN106307604A (en) * 2016-11-22 2017-01-11 中国烟草总公司郑州烟草研究院 Tobacco extract, extracting method and application

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0568526A (en) * 1991-09-12 1993-03-23 T Hasegawa Co Ltd Preparation of extract for tobacco flavoring
CN1400300A (en) * 2001-08-01 2003-03-05 云南瑞升科技有限公司 Method for deep-processing tobacco extract to prepare tobacco cleaned oil and enriched solanesol
EP1668113B1 (en) * 2003-08-11 2013-06-19 Verenium Corporation Laccases, nucleic acids encoding them and methods for making and using them
CN1732813A (en) * 2005-09-12 2006-02-15 华宝食用香精香料（上海）有限公司 Method for preparing tobacco extractive by biological enzyme
CN1732812A (en) * 2005-09-12 2006-02-15 华宝食用香精香料（上海）有限公司 Method for improving tobacco extractive incense by multiple biological enzymes
CN101284766A (en) * 2008-06-04 2008-10-15 云南瑞升烟草技术（集团）有限公司 Process for finely purifying high-purity solanesol form coarse cream of solanesol
CN102178346A (en) * 2011-05-30 2011-09-14 昌宁德康生物科技有限公司深圳分公司 Production method for extracting absolute oil of tobacco leaves
CN103627525A (en) * 2013-09-11 2014-03-12 上海聚华科技股份有限公司 Method for preparing tobacco essential oil by reduced pressure distillation
CN105256654A (en) * 2014-06-09 2016-01-20 云南红塔蓝鹰纸业有限公司 Cigarette paper having cigarette sucking taste improving function, and preparation method thereof
CN104872814A (en) * 2015-05-05 2015-09-02 昌宁德康生物科技有限公司 Production method for extracting tobacco leaf clean oil
CN105105321A (en) * 2015-10-14 2015-12-02 中国烟草总公司郑州烟草研究院 Electronic cigarette tobacco extract without sweet greasy taste and electronic liquid
CN106047491A (en) * 2016-05-09 2016-10-26 南京泽朗医药科技有限公司 An extracting method for oregano volatile oil
CN106221935A (en) * 2016-08-26 2016-12-14 云南养瑞科技集团有限公司 The preparation method of a kind of Pericarpium Citri Reticulatae absolute oil and the application in Medicated cigarette thereof
CN106307604A (en) * 2016-11-22 2017-01-11 中国烟草总公司郑州烟草研究院 Tobacco extract, extracting method and application

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109007955A (en) * 2018-06-21 2018-12-18 重庆中烟工业有限责任公司 A kind of cabo extractive, preparation method and tobacco product
CN109007955B (en) * 2018-06-21 2021-12-31 重庆中烟工业有限责任公司 Tobacco stem extract, preparation method thereof and tobacco product
CN109280581A (en) * 2018-09-20 2019-01-29 云南中烟工业有限责任公司 A kind of high efficiency preparation method and purposes of pure tobacco oil
CN109280581B (en) * 2018-09-20 2021-07-30 云南中烟工业有限责任公司 Efficient preparation method and application of tobacco absolute
CN112471586A (en) * 2020-11-20 2021-03-12 河北中烟工业有限责任公司 Method for simultaneously preparing tobacco essential oil and tobacco juice
CN115088860A (en) * 2022-07-05 2022-09-23 贵州黄果树金叶科技有限公司 Method for preparing tobacco absolute oil by treating tobacco waste through enzymolysis, tobacco absolute oil and application

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Application publication date: 20180316