CN107794574B - 一种构建dna大片段文库的方法及其应用 - Google Patents
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Abstract
Description
目标片段大小 | ON/OFF时间(秒) | 循环数 |
300-400bp | 15/30 | 10 |
Claims (14)
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CN201610791196X | 2016-08-31 | ||
CN201610791196 | 2016-08-31 |
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CN107794574B true CN107794574B (zh) | 2022-10-25 |
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Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102181943A (zh) * | 2011-03-02 | 2011-09-14 | 中山大学 | 一种配对双末端文库构建方法及用该文库进行基因组测序的方法 |
CN102296065A (zh) * | 2011-08-04 | 2011-12-28 | 盛司潼 | 用于构建测序文库的***与方法 |
CN102628079A (zh) * | 2012-03-31 | 2012-08-08 | 盛司潼 | 一种通过环化方式构建测序文库的方法 |
CN102864498A (zh) * | 2012-09-24 | 2013-01-09 | 天津工业生物技术研究所 | 一种长片段末端文库的构建方法 |
CN102943074A (zh) * | 2012-10-25 | 2013-02-27 | 盛司潼 | 一种接头和构建测序文库的方法 |
CN103667273A (zh) * | 2013-12-05 | 2014-03-26 | 北京诺禾致源生物信息科技有限公司 | 双链接头、其应用及构建末端配对dna文库的方法 |
CN104153003A (zh) * | 2014-08-08 | 2014-11-19 | 上海美吉生物医药科技有限公司 | 一种基于illumina测序平台的大片段DNA文库的构建方法 |
WO2016058136A1 (zh) * | 2014-10-14 | 2016-04-21 | 深圳华大基因科技有限公司 | 一种接头元件和使用其构建测序文库的方法 |
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- 2016-11-23 CN CN201611046530.5A patent/CN107794574B/zh active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102181943A (zh) * | 2011-03-02 | 2011-09-14 | 中山大学 | 一种配对双末端文库构建方法及用该文库进行基因组测序的方法 |
CN102296065A (zh) * | 2011-08-04 | 2011-12-28 | 盛司潼 | 用于构建测序文库的***与方法 |
CN102628079A (zh) * | 2012-03-31 | 2012-08-08 | 盛司潼 | 一种通过环化方式构建测序文库的方法 |
CN102864498A (zh) * | 2012-09-24 | 2013-01-09 | 天津工业生物技术研究所 | 一种长片段末端文库的构建方法 |
CN102943074A (zh) * | 2012-10-25 | 2013-02-27 | 盛司潼 | 一种接头和构建测序文库的方法 |
CN103667273A (zh) * | 2013-12-05 | 2014-03-26 | 北京诺禾致源生物信息科技有限公司 | 双链接头、其应用及构建末端配对dna文库的方法 |
CN104153003A (zh) * | 2014-08-08 | 2014-11-19 | 上海美吉生物医药科技有限公司 | 一种基于illumina测序平台的大片段DNA文库的构建方法 |
WO2016058136A1 (zh) * | 2014-10-14 | 2016-04-21 | 深圳华大基因科技有限公司 | 一种接头元件和使用其构建测序文库的方法 |
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