CN107792837B - Method for preparing nano-selenium by utilizing camellia plant nano-aggregate and nano-selenium prepared by method - Google Patents
Method for preparing nano-selenium by utilizing camellia plant nano-aggregate and nano-selenium prepared by method Download PDFInfo
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Abstract
The invention belongs to the technical field of nano materials, and discloses a method for preparing nano selenium by utilizing camellia plant nano aggregates. The method comprises the following steps: (1) separating the nano aggregates of the camellia plants: preparing a camellia plant water extract, and separating camellia plant nano aggregates by ultrafiltration and centrifugation, wherein the average diameter is more than 10nm and the average diameter is less than or equal to 10 nm; (2) preparing nano selenium by using camellia plant nano aggregates: sodium selenite and vitamin C are subjected to reduction reaction in the presence of the camellia plant nano aggregate to obtain nano selenium sol, and vitamin C is removed to obtain a nano selenium finished product. The invention discloses the difference of nano-selenium of different camellia plant nano-aggregates by taking the camellia plant nano-aggregates as a template, enhances the functional characteristics of the nano-selenium, widens the application field of the nano-selenium and improves the application value of the nano-selenium.
Description
Technical Field
The invention belongs to the technical field of nano materials, and particularly relates to a method for preparing nano selenium by utilizing camellia plant nano aggregates and the prepared nano selenium.
Background
Selenium (Se) is one of essential trace elements for human body and is closely related to human health. Antioxidation and anticancer are the most important biological activities of selenium. Selenium also has effects of enhancing immunity, antagonizing heavy metals, participating in iodine metabolism in vivo, and regulating iodine level and free radical level in organism. Selenium is a double-edged sword, and has toxicity while exerting antioxidant and anticancer activities. The biological effects of selenium and the toxic concentration of selenium are extremely narrow, exhibiting toxicity slightly in excess of nutritional doses. The research finds that the toxicity of selenium and selenide is as follows: inorganic selenium, organic selenium and simple substance selenium.
In nature, elemental selenium exists mainly in three forms: gray selenium, black selenium and red elemental selenium. The red element selenium is in the nanometer size range and is called nano selenium. The research shows that: compared with inorganic selenium and organic selenium, nano-selenium has high biological activity and low toxicity, but the instability limits the application range of the nano-selenium, so that the research of searching a preparation method of the high-stability nano-selenium becomes the mainstream.
The preparation method of the nano-selenium comprises the following steps: polymer template method, solid phase reaction method, micro emulsion method, ultrasonic chemical method and temperature control method. The polymer template method can endow the template function to the nano-selenium, has mild reaction conditions, simple operation and controllable particle size, and becomes a common method for preparing the nano-selenium. Therefore, the selection of the template is extremely important.
The current commonly used templates are mainly functional active ingredients, surfactants and the like, and comprise: functional polysaccharide, protein and polypeptide, etc.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention mainly aims to provide a method for preparing nano selenium by using camellia plant nano aggregates; the method takes nano aggregates from different camellia plants as templates to prepare nano selenium with different particle sizes, and the particle size of the nano selenium is positively correlated with the particle size of the templates; in addition, the active components and contents of the nano-aggregates of different camellia plants are different, so that the functional activities of the nano-aggregates are different, the nano-selenium of the camellia plants with enhanced functions is prepared, and the nano-selenium of the camellia plants can be applied to different health care fields.
The invention also aims to provide the nano selenium prepared by the method.
The purpose of the invention is realized by the following technical scheme:
a method for preparing nano selenium by using camellia plant nano aggregates comprises the following steps:
(1) separating the camellia plant nano-aggregates;
(2) the nano-selenium is prepared by utilizing the nano-aggregate of the camellia plant.
The method for separating the camellia plant nano aggregate in the step (1) specifically comprises the following steps: adding 25-100 ℃ water into camellia plant raw materials according to a material-liquid ratio of 1: 10-1: 250, then leaching for 2-120 min under a 25-100 ℃ water bath condition to obtain camellia plant extract, centrifuging for 10-40 min at 4 ℃ and 1000-4000 g of centrifugal force by using a centrifugal ultrafiltration tube with the molecular weight cutoff of 3-100 kDa, collecting an inner tube and an outer tube to obtain nano aggregate solutions with different particle size ranges, freezing, drying, and storing at-20 ℃ to obtain the camellia plant nano aggregate.
The Camellia plant material is Theaceae (Theaceae) plant material, including folium Camelliae sinensis (Camellia sinensis) or cacao tea (Camellia ptilophylla).
More preferably, the feed-liquid ratio is 1: 50; the temperature of the water bath is 100 ℃; the leaching time is 30 min; the molecular weight cut-off of the centrifugal ultrafiltration tube is 100 kD; the centrifugal force is 4000 g; the centrifugation time is 20 min; the obtained camellia plant nano aggregate is two types of colloidal particles with the average diameter more than 10nm and the average diameter less than or equal to 10 nm.
The preparation of the nano-selenium by utilizing the camellia plant nano-aggregate in the step (2) specifically comprises the following steps:
A. carrying out reduction reaction on a sodium selenite solution and a vitamin C solution in a molar ratio of 1: 2-1: 20 in the presence of a camellia plant nano aggregate, and standing in a water bath at 20-80 ℃ for 0.5-72 h to obtain nano selenium sol; the concentration of the sodium selenite solution is 10-100 mM, and the concentration of the vitamin C is 100-500 mM; the addition amount of the camellia plant nano aggregate is 25-900 mg/L;
B. removing vitamin C from the nano selenium sol by high-speed centrifugal separation, ultrafiltration centrifugal separation or dialysis separation to obtain nano selenium suspension, and freeze-drying to obtain solid nano selenium; the high-speed centrifugal separation is carried out for 20-40 min at the rotating speed of 8000-11000 r/min at the temperature of 4 ℃, the ultrafiltration centrifugal separation is carried out for 10-40 min at the rotating speed of 1000-4000 g by adopting a 3-100 kDa centrifugal ultrafiltration tube at the temperature of 4 ℃, and the dialysis separation is carried out for 12-96 h by adopting a 3.5-8000 kDa regenerated cellulose dialysis bag.
The concentration of the sodium selenite solution in the step A is 10mM, and the concentration of the vitamin C is 100 mM; the molar ratio of the sodium selenite solution to the vitamin C solution is 1: 8; the addition amount of the camellia plant nano aggregate is 25mg/L or 50 mg/L; the temperature of the water bath is 25 ℃, and the standing time is 24 h.
Nano-selenium prepared according to the method.
Detecting the survival rate of tumor cells by an MTT method, and evaluating the anticancer activity of the nano-selenium obtained by the invention; the anti-inflammatory activity of the nano-selenium obtained by the invention is evaluated by a chemical anti-inflammatory method and an inflammatory cell model.
The tumor cell model comprises: HCT 116 cell line, Hepa1c1c7 cell line, MDA-MB-231 cell line, HepG2 cell line, Hela cell line, etc., preferably HCT 116 cell line.
The chemical anti-inflammatory method is referred to as Tsai and the like.
The inflammatory cell model comprises: raw264.7 cell line, Caco-2 cell line, HUVEC cell line, etc., preferably Raw264.7 cell line.
The anti-inflammatory activity assay described above comprises: chemical anti-inflammatory and cellular methods, preferably cellular methods.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the invention adopts the camellia plant nano aggregate as a template; the camellia plant contains a large amount of polyphenols, saponins, alkaloids and other functional active ingredients, and has the health effects of resisting oxidation, mutation, cancer, inflammation and blood fat; the nano aggregate is nano colloidal particles formed by aggregating molecules through a certain acting force when a compound reaches a certain critical concentration in a solution, is an intermediate state of the compound between free molecules and precipitated particles, is a basic unit for enriching active ingredients, and is one of important result bases for different compounds to exert a synergistic/antagonistic effect; taking the tea nano aggregate (Camellia sinensis) of the family Theaceae as an example, the tea nano aggregate takes protein, tea polyphenol and oxides thereof as main components; the tea polyphenol and the oxide thereof contain a large number of hydroxyl groups and hydrophobic regions, the protein contains amino groups, carboxyl groups, carbonyl groups and the like, and each group can be combined with the nano-selenium through electrostatic acting force or hydrophobic interaction to fully disperse and stabilize the nano-selenium; in addition, tea polyphenol and the like are the material basis of the health care effect of tea, and the nano selenium is prepared by taking the tea nano aggregate as a template, so that the nano selenium tea is endowed with the effect, and the antioxidant and anticancer activities of the nano selenium tea are synergistic.
Drawings
FIG. 1 shows the effect of different reactant ratios and reaction times on the particle size of nano-selenium.
FIG. 2 is a graph showing the effect of different template addition amounts on the particle size of nano-selenium.
FIG. 3 is a graph showing the effect of different separation processes on the particle size of nano-selenium.
FIG. 4 is a graph showing the effect of different reactant concentrations on the particle size of nano-selenium.
FIG. 5 shows the particle size of nano-selenium using nano-aggregates (average diameter > 10nm) of dry tea processed by different primary processing techniques as a template.
FIG. 6 shows the particle size of nano-selenium using nano-aggregates (average diameter less than or equal to 10nm) of dry tea processed by different primary processing techniques as a template.
Detailed Description
The present invention will be described in further detail with reference to examples and drawings, but the present invention is not limited thereto.
In order to make the purpose and technical scheme of the present invention more clear, the present invention is further described in detail below by taking nano aggregates of fresh tea (Camellia sinensis) and fresh cocoa (Camellia ptilophylla) processed into dry tea by different primary processing techniques as templates. The equipment and the reagent used in the invention are all commonly used in the field. It should be understood that the examples described herein are for illustrative purposes only and are not intended to limit the present invention.
Example 1: a method for preparing nanometer selenium from nanometer aggregate of Camellia japonica is provided.
(1) Accurately weighing 4.5g of green tea in a 250mL conical flask, adding 225mL of boiling water, boiling in water bath for 30min, oscillating every 10min, filtering while hot, cooling and fixing the volume.
(2) Transferring 4mL of tea soup intoCentrifuging the inner tube of 100KDa ultrafilter tube at 4 deg.C and 4000g for 20 min; the inner/outer tube solutions were collected to different particle size ranges (D)H> 10nm and DHLess than or equal to 10nm), and freeze-drying to obtain freeze-dried powder for later use.
(3) 10mM sodium selenite solution and 100mM vitamin C solution are prepared respectively.
(4) Weighing green tea nanometer aggregate lyophilized powder (D)H> 10nm)40mg (400mg/L) were transferred to a beaker using 10mL of ultrapure water in a 5mL centrifuge tube, 72mL of ultrapure water were added, stirred well, and filtered through a 0.8 μm filter. The concentration of sodium selenite in the fixed system is 1mM, the sodium selenite is added into a certain amount of 10mM sodium selenite solution according to different reactant proportions respectively, Vc is 2:1, 3:1, 4:1, 5:1, 6:1, 8:1, 10:1 and 20:1(mmol: mmol), the sodium selenite solution is mixed uniformly and then stands for 15min, then a certain amount of 100mM vitamin C solution is added, the mixture is mixed uniformly and reacts and stands for 24h, and the nano-selenium colloid solution is obtained;
(5) and dialyzing the nano selenium colloidal solution for 24 hours by a 3.5kDa regenerated cellulose dialysis bag to remove unreacted vitamin C, and determining the chemical characteristics of the nano selenium colloidal solution colloid, wherein the results are shown in figure 1, and when the reactant ratio is 8:1, the average diameter of nano selenium is smaller, the number of particles is larger, and the system is more stable.
(6) Freeze drying the dialyzed nano selenium colloidal solution to obtain solid nano selenium powder, and preserving at-20 ℃ for later use.
Example 2: a method for preparing nanometer selenium from nanometer aggregate of Camellia japonica is provided.
Compared with example 1, the difference is only that:
weighing green tea nanometer aggregate lyophilized powder (D)H> 10nm)2.5, 5, 10, 20, 30, 40, 50mg (25, 50, 100, 200, 300, 400, 500, 600mg/L) in a 5mL centrifuge tube, transferring to a beaker with 10mL ultrapure water, adding 72mL ultrapure water, stirring well, and filtering through a 0.8 μm filter membrane. The concentration of the sodium selenite in the fixed system is 1mM, according to the reactant proportion, Vc, sodium selenite 8:1(mmol: mmol) is added into a certain amount of 10mM sodium selenite solution, the mixture is evenly mixed and then stands for 15min, then a certain amount of 100mM vitamin C solution is added, the mixture is evenly mixed, and the mixture is reacted and stands forAnd (3) obtaining a nano-selenium colloidal solution after 24h, measuring the chemical characteristics of the colloid, and taking the comprehensive consideration as shown in figure 2, wherein when the template concentration is 25mg/L and 50mg/L, the average diameter of nano-selenium is smaller, the nano-selenium suspension is free from coagulation, and the system is more stable.
Example 3: a method for preparing nanometer selenium from nanometer aggregate of Camellia japonica is provided.
Compared with example 1, the difference is only that:
weighing green tea nanometer aggregate lyophilized powder (D)H> 10nm)40mg (400mg/L) were transferred to a beaker using 10mL of ultrapure water in a 5mL centrifuge tube, 72mL of ultrapure water were added, stirred well, and filtered through a 0.8 μm filter. The concentration of sodium selenite in the fixed system is 1mM, according to the reactant proportion, Vc, sodium selenite 8:1(mmol: mmol) is added with a certain amount of 10mM sodium selenite solution, the sodium selenite solution is uniformly mixed and then stands for 15min, then a certain amount of 100mM vitamin C solution is added, the sodium selenite solution is uniformly mixed and stands for 4, 6, 8, 12, 24, 36, 48 and 72h to obtain nano-selenium colloidal solution, the colloidal chemical characteristics of the nano-selenium colloidal solution are measured, and the results are shown in figure 1, when the reaction time is 24h, the average diameter of nano-selenium is smaller, the total light intensity of the system is larger, and the system is more stable.
Example 4: a method for preparing nanometer selenium from nanometer aggregate of Camellia japonica is provided.
Compared with example 1, the difference is only that:
weighing green tea nanometer aggregate lyophilized powder (D)H> 10nm)2.5mg (25mg/L) were transferred to a beaker using 10mL of ultrapure water in a 5mL centrifuge tube, 72mL of ultrapure water was added, stirred well, and filtered through a 0.8 μm filter. The concentration of the sodium selenite in the fixed system is 1mM, according to the reactant proportion, Vc is sodium selenite 8:1(mmol: mmol) and is added with a certain amount of 10mM sodium selenite solution, the mixture is evenly mixed and then stands for 15min, then a certain amount of 100mM vitamin C solution is added, the mixture is evenly mixed and reacts and stands for 24h, and the nano-selenium colloid solution is obtained;
and (3) carrying out freezing high-speed centrifugation on the nano selenium colloidal solution for 40min at 4 ℃, 11000rpm/min, carrying out ultrafiltration centrifugal separation for 20min at 4 ℃ at 4000g or dialyzing the regenerated cellulose dialysis bag of 3.5kDa for 24h to remove unreacted vitamin C, and determining the chemical characteristics of the nano selenium colloidal solution colloid, wherein the result is shown in figure 3, the influence of dialysis separation on the average diameter of nano selenium is minimum, and the average diameters before and after dialysis have no significant difference.
Example 5: a method for preparing nanometer selenium from nanometer aggregate of Camellia japonica is provided.
(1) 100mM sodium selenite solution and 500mM vitamin C solution are prepared respectively.
(2) Weighing green tea nanometer aggregate lyophilized powder (D)H> 10nm)2.5, 12.5, 25, 37.5, 50mg (template addition amount at the same magnification as reactant concentration: 5 times, 10 times, 15 times, 20 times) in a 5mL centrifuge tube, transferring to a beaker with 10mL ultrapure water, adding 72mL ultrapure water, stirring well, and filtering through a 0.8 μm filter membrane. Adding a certain amount of 100mM sodium selenite solution into Vc, sodium selenite 8:1, sodium selenite 40:5, sodium selenite 80:10, sodium selenite 120:15 and sodium selenite 160:20(mmol: mmol) according to the mixture ratio of reactants, uniformly mixing, standing for 15min, then adding a certain amount of 500mM vitamin C solution, uniformly mixing, reacting and standing for 24h to obtain a nano-selenium colloid solution;
(3) and dialyzing the nano selenium colloidal solution for 24 hours by a 3.5kDa regenerated cellulose dialysis bag to remove unreacted vitamin C, and determining the chemical characteristics of the nano selenium colloidal solution colloid, wherein the results are shown in figure 4, the average diameter of nano selenium is reduced and the number of nano selenium particles is increased along with the increase of the concentration of reactants, but the system stability is reduced, so the reactant proportion is preferably 8: 1.
(4) Freeze drying the dialyzed nano selenium colloidal solution to obtain solid nano selenium powder, and preserving at-20 ℃ for later use.
Example 6: a method for preparing nanometer selenium from nanometer aggregate of Camellia japonica is provided.
Compared with example 1, the difference is only that:
accurately weighing 4.5g of green tea, white tea, yellow tea, oolong tea, black tea, Pu' er tea, cocoa green tea and cocoa black tea respectively, placing in a 250mL conical flask, adding 225mL of boiling water, boiling in water bath for 30min, oscillating every 10min, hot filtering, cooling, and fixing volume.
Respectively weighing green tea, white tea, yellow tea, oolong tea, black tea, Pu her tea, cocoa green tea, and cocoa black tea nanometer aggregate (D)H> 10nm and DHLess than or equal to 10nm) freeze-dried powder 2.5mg and 5mg are placed in a 5mL centrifuge tube, 10mL ultrapure water is transferred into a beaker, 72mL ultrapure water is added, the mixture is uniformly stirred, the filtrate passes through a 0.8 mu m filter membrane, 10mL 10mM sodium selenite solution is added, the mixture is uniformly mixed and then stands for 15min, 8mL 100mM vitamin C solution is added, the mixture is uniformly mixed, the reaction and the standing are carried out for 24h, and a nano-selenium colloid solution is obtained, wherein the colloid chemical characteristic results are shown in figure 5 and figure 6, and when the template addition concentration is 25mg/L, the average diameter of nano-selenium is smaller; the average diameter of the nano-selenium prepared by using the tea soup nano-aggregate with the average diameter less than or equal to 10nm as the template is smaller at the same template concentration. The average diameter size of the nano selenium is related to the particle size of the template.
Example 7: determination of anticancer activity of nano selenium
Adherent HCT 116 cells were trypsinized to make single cell suspensions at 5X 104Inoculating 96-well culture plate to each cell/well, and culturing at 37 deg.C under 5% CO2Pre-culturing in incubator for 24 hr, and processing tea soup nanometer aggregate of dry tea by different primary processing method (D)HMore than 10nm) as template (the template amount is 25mg and 50mg respectively), dissolving in culture medium, adding culture medium at a rate of 100 μ L/well, and culturing for 72 h. 0.05mg/mL MTT dilution 100. mu.L/well was added to the plate, which was left at 37 ℃ with 5% CO2Continuously culturing for 2h in the incubator, removing supernatant, adding 200 mu L DMSO solution into each hole, placing in a shaking table at 100rpm/min, shaking for 15-20 min, and measuring OD of each hole550Value as control OD550The value is 100%, and the half Inhibitory Concentration (IC) of the nano-selenium for inhibiting cell proliferation is calculated50) As shown in Table 1, when the template is added in an amount of 25mg/L or 50mg/L, green tea, white tea, yellow tea, oolong tea, Pu' er tea, cacao green tea D is addedHThe nano-selenium prepared by using nano-aggregates with the particle size of more than 10nm as templates has stronger antiproliferative activity, and particularly, the nano-selenium prepared by using the green tea nano-aggregates as templates has the strongest anticancer activity.
TABLE 1 processing of dried tea Nano-aggregates (D) in different Primary processingH> 10nm) of semi-Inhibitory Concentration (IC) of nano-selenium for inhibiting HCT 116 cell proliferation by using nano-selenium as template50)
(sample concentration gradient is 1.5625, 3.125, 6.25, 12.5, 25, 50 mug/mL; nano selenium using black tea and cocoa black tea nano aggregate as template has cell survival rate of more than 0.5 in the concentration range)
Example 8: determination of anti-inflammatory activity of nano-selenium
Scraping adherent Raw264.7 cells off by a scraper, blowing and dispersing into single cell suspension at 5X 105Inoculating 96-well culture plate to each cell/well, and culturing at 37 deg.C under 5% CO2Pre-culturing in incubator for 24 hr, and processing tea soup nanometer aggregate of dry tea by different primary processing method (D)HLess than or equal to 10nm) as a template (the template amount is 25mg), dissolving the nano-selenium with a culture medium, adding the culture medium according to 100 mu L/hole, adding 100 mu L/hole of LPS working solution with 1 mu g/mL, and continuing culturing for 24 h. Respectively taking out 100 μ L/well of sample culture medium from the culture plate, adding Griess reagent 100 μ L/well, standing for 10min, and determining OD542And (4) calculating the inhibition rate of the nano selenium in each group on the generation of NO. After discarding the culture medium from the old plate, 0.05mg/mL MTT dilution (100. mu.L/well) was added thereto, and the plate was left at 37 ℃ with 5% CO2Continuously culturing for 1h in the incubator, removing supernatant, adding 200 mu L DMSO solution into each hole, placing in a shaking table at 100rpm/min, shaking for 10-15 min, and measuring OD of each hole550Value as control OD550The cell viability was calculated at a value of 100%. By cell survival rate>50 percent, calculating the half Inhibition Concentration (IC) of each group of nano-selenium for inhibiting NO generation50) As shown in Table 2, the nano-aggregates (D) of green tea, white tea, oolong tea, cocoa tea, and black tea were preparedHLess than or equal to 10nm) as template, has strong anti-inflammatory activity.
TABLE 2 processing of dried tea Nano aggregates (D) in different Primary processingHLess than or equal to 10nm) as template to semi-Inhibitory Concentration (IC) of Raw264.7 cell NO production50)
(the sample concentration gradient is 3.125, 6.25, 12.5, 25, 50 and 100 mu g/mL; the NO inhibition rate of the nano selenium taking the nano aggregates of the yellow tea, the black tea and the Pu' er tea as the template is lower than 0.5 in the concentration range.)
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (5)
1. A method for preparing nano selenium by using camellia plant nano aggregates is characterized by comprising the following steps:
(1) separating the camellia plant nano-aggregates;
(2) preparing nano selenium by using camellia plant nano aggregates;
the method for separating the camellia plant nano aggregate in the step (1) specifically comprises the following steps: adding 25-100 ℃ water into camellia plant raw materials according to a material-liquid ratio of 1: 10-1: 250, then leaching for 2-120 min under a 25-100 ℃ water bath condition to obtain camellia plant extract, centrifuging for 10-40 min at 4 ℃ and 1000-4000 g of centrifugal force by using a centrifugal ultrafiltration tube with the molecular weight cutoff of 3-100 kDa, collecting an inner tube and an outer tube to obtain nano aggregate solutions with different particle size ranges, freezing and drying, and storing at-20 ℃ to obtain camellia plant nano aggregates;
the preparation of the nano-selenium by utilizing the camellia plant nano-aggregate in the step (2) specifically comprises the following steps:
A. carrying out reduction reaction on a sodium selenite solution and a vitamin C solution in a molar ratio of 1: 2-1: 20 in the presence of a camellia plant nano aggregate, and standing in a water bath at 20-80 ℃ for 0.5-72 h to obtain nano selenium sol; the concentration of the sodium selenite solution is 10-100 mM, and the concentration of the vitamin C is 100-500 mM; the addition amount of the camellia plant nano aggregate is 25-900 mg/L;
B. removing vitamin C from the nano selenium sol by high-speed centrifugal separation, ultrafiltration centrifugal separation or dialysis separation to obtain nano selenium suspension, and freeze-drying to obtain solid nano selenium; the high-speed centrifugal separation is carried out for 20-40 min at the rotating speed of 8000-11000 r/min at the temperature of 4 ℃, the ultrafiltration centrifugal separation is carried out for 10-40 min at the rotating speed of 1000-4000 g by adopting a 3-100 kDa centrifugal ultrafiltration tube at the temperature of 4 ℃, and the dialysis separation is carried out for 12-96 h by adopting a 3.5-8000 kDa regenerated cellulose dialysis bag.
2. The method for preparing nano-selenium by using nano-aggregates of camellia plants as claimed in claim 1, wherein: the camellia plant raw material is tea.
3. The method for preparing nano-selenium by using nano-aggregates of camellia plants as claimed in claim 1, wherein: the material-liquid ratio is 1: 50; the temperature of the water bath is 100 ℃; the leaching time is 30 min; the molecular weight cut-off of the centrifugal ultrafiltration tube is 100 kD; the centrifugal force is 4000 g; the centrifugation time is 20 min; the obtained camellia plant nano aggregate is two types of colloidal particles with the average diameter more than 10nm and the average diameter less than or equal to 10 nm.
4. The method for preparing nano-selenium by using nano-aggregates of camellia plants as claimed in claim 1, wherein: the concentration of the sodium selenite solution in the step A is 10mM, and the concentration of the vitamin C is 100 mM; the molar ratio of the sodium selenite solution to the vitamin C solution is 1: 8; the addition amount of the camellia plant nano aggregate is 25mg/L or 50 mg/L; the temperature of the water bath is 25 ℃, and the standing time is 24 h.
5. A nano-selenium produced by the method of any one of claims 1 to 4.
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| CN103876158B (en) * | 2013-11-18 | 2016-09-28 | 国家***第三海洋研究所 | A kind of elemental selenium and sugared complex and preparation method thereof |
| JP6304530B2 (en) * | 2013-12-13 | 2018-04-04 | 三菱マテリアル株式会社 | Tellurium separation and recovery method |
| CN105111328B (en) * | 2015-10-14 | 2017-08-11 | 四川农业大学 | A kind of preparation method of the luxuriant mushroom polysaccharide of selenic acid esterification shuttle handle pine |
| CN106539092B (en) * | 2016-10-13 | 2021-02-05 | 暨南大学 | Chlorella polysaccharide nano-selenium and preparation method and application thereof |
| CN106589157A (en) * | 2016-12-21 | 2017-04-26 | 福建农林大学 | Selenium modification method for improving immunological competence of radix pseudostellariae polysaccharides |
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