CN107772195A - One kind poultry poultry fodder takes off mould dose and preparation method thereof - Google Patents

One kind poultry poultry fodder takes off mould dose and preparation method thereof Download PDF

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Publication number
CN107772195A
CN107772195A CN201610742304.4A CN201610742304A CN107772195A CN 107772195 A CN107772195 A CN 107772195A CN 201610742304 A CN201610742304 A CN 201610742304A CN 107772195 A CN107772195 A CN 107772195A
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CN
China
Prior art keywords
poultry
mould dose
cell wall
yeast cell
takes
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Pending
Application number
CN201610742304.4A
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Chinese (zh)
Inventor
高明燕
李旭东
刘爱玲
王莹
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Tianjin Ringpu Bio Technology Co Ltd
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Tianjin Ringpu Bio Technology Co Ltd
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Priority to CN201610742304.4A priority Critical patent/CN107772195A/en
Publication of CN107772195A publication Critical patent/CN107772195A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3571Microorganisms; Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3562Sugars; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/358Inorganic compounds

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Inorganic Chemistry (AREA)
  • Fodder In General (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

It is feeding de- mould dose and preparation method thereof the invention discloses one kind, belong to feed additive field.Described de- mould dose containing glucose oxidase, yeast cell wall, clinoptilolite, probiotic group into.De- mould dose of the present invention can be used for feedstuff and the mixed feed detoxifications such as the corn by mycotoxin contamination, cereal, prevent the generation of livestock and poultry various diseases caused by using mycotoxin contamination feed.The present invention seeks to reduce absorption of the animal body to mycotoxin, while strengthen the dispelling toxicity, detoxicating ability and capacity of self-regulation of animal body itself.The present invention has significant effect, safe to use, quality controllable, technique simple and stable.

Description

One kind poultry poultry fodder takes off mould dose and preparation method thereof
Technical field
It is feeding de- mould dose and preparation method thereof the present invention relates to one kind, belong to feed additive field.
Background technology
In aquaculture, feed goes mouldy the problem of being one regular, after feed mold, it may appear that and taste is abnormal, Fatty acidity is high, and nutrient is greatly destroyed, while palatability is also drastically deteriorated, and one is produced after most importantly wherein going mouldy The toxin of series, for animal, can serious its health of influence, most important problem concentrates on liver region, has Can be with general hemorrhage, the disorders of digestion and nervous symptoms are principal character.So as to also jeopardize the matter of animal product Amount.The problem of this is one extremely important and urgent.
The main product of the absorbing mycotoxin based on montmorillonite, yeast cell wall currently on the market, it is to mould poison Be known as certain suction-operated, but effect can't reach perfect condition, moreover, by mycotoxin harm animal in itself Body cannot recover well, just current situation, and present invention employs clinoptilolite, yeast cell wall, grape are glycoxidative Enzyme, probiotic combination effect, it can be very good absorption and go mouldy the mycotoxin of feed, while have more to the immune of animal body Good lifting, so that body reaches a healthy situation.
Montmorillonite and yeast cell wall can utilize physical space structure absorbing mycotoxin, and glucose oxidase is simultaneously Animal body caused harmful substance in metabolic process can be eliminated, is oxygen radical, the natural enemy of toxin in animal body, Other probiotics and yeast cell wall can lift the immunity of organisms of animal, so as to from comprehensive de- mould.
The content of the invention
It is feeding de- mould dose it is an object of the invention to provide one kind, the aflatoxin in feedstuff can be effectively removed, The a variety of toxin of zearalenone, T2 toxin, vomitoxin etc., prevent the feed of livestock and poultry domestic fungus endotoxin contamination and trigger Various health problems.
One kind poultry poultry fodder takes off mould dose, and it mainly includes:It is glucose oxidase, yeast cell wall, clinoptilolite, prebiotic Bacterium.
Every gram of probiotics viable bacteria is 3 × 10 in composition7-3×1010Individual, glucose oxidase accounts for the 1%- of gross mass 30%, clinoptilolite is 0.5%-5%, and remaining is yeast cell wall.
Described probiotics includes saccharomycete, bacillus subtilis, lactic acid bacteria, forms and is expressed as ferment with the ratio between number of viable Female bacterium:Bacillus subtilis:Lactic acid bacteria=1:1:1.
Described lactic acid bacteria is VREF, enterococcus faecalis, lactobacillus acidophilus, one kind of Lactobacillus plantarum or its combination.
The present invention produces by the following method:
(1) according to 3 × 107-3×1010Cfu/g ratio weighs probiotics, according to the principle clinoptilolite of doubling dilution Dilution mixes;
(2) add such as yeast cell wall, glucose oxidase again, mix, produce.
The beneficial effects of the invention are as follows:
The de- mould dose harm that can prevent mycotoxin comprehensively of the present invention, on the one hand can effectively be removed each in feed Kind toxin, the absorption of mycotoxin in vivo is prevented, the discharge for having aspect to promote mycotoxin in animal body in addition, is kept away Exempt from the harm of mycotoxin.The mycotoxin of the present invention can strengthen immune system simultaneously, lift the immunity function of livestock and poultry, increase The resistance against diseases of animal.
Embodiment
Following examples are to illustrate the preferred embodiment of the present invention.
With the following Examples 1-3 be observed that the present invention beneficial property, these embodiments be it is illustrative, will not Limit the invention.And probiotics is bought for market, saccharomycete bacterium number is 20,000,000,000 cfu/g.Bacillus subtilis and lactic acid Bacterium is 100,000,000,000 cfu/g.
Embodiment 1
(1) saccharomycete 50g, bacillus subtilis 10g, Lactobacillus plantarum 10g, clinoptilolite 0.5kg, grape glycosyloxy are weighed Change enzyme 30kg, 69.43kg yeast cell wall;
(2) saccharomycete, bacillus subtilis, Lactobacillus plantarum mix, dilute according to the principle clinoptilolite of doubling dilution Release mixing;
(3) glucose oxidase, yeast cell wall are added, mixes, produces.
Embodiment 2
(1) saccharomycete 50kg, bacillus subtilis 10kg, VREF 10kg weighed, and clinoptilolite 5kg, grape is glycoxidative Enzyme 1kg, 24kg yeast cell wall;
(2) clinoptilolite uniformly mixes with yeast cell wall;
(3) glucose oxidase, yeast cell wall are added, mixes, produces.
Embodiment 3
(1) saccharomycete 2.5kg, bacillus subtilis 0.5kg, Lactobacillus plantarum 0.5kg, clinoptilolite 2.0kg, Portugal are weighed Grape carbohydrate oxidase 15kg, 80.5kg yeast cell wall;
(2) saccharomycete, bacillus subtilis, Lactobacillus plantarum, clinoptilolite mix, and are used according to the principle of doubling dilution Yeast cell wall dilution mixes;
(3) glucose oxidase, yeast cell wall are added, mixes, produces.
Embodiment 4:Feed of the present invention takes off mould dose of compliance test result test:
Mouse is randomly divided into 5 groups, every group 10, and first group is blank control group, and second group is test control group, 3-5 Group is embodiment group, is tested after 30d, illustrates and see the table below 1.Feeding self-control feed, Mouse feeder are 25 ± 3 in temperature DEG C, relative humidity 50%-55%, natural lighting.
Experiment the previous day each group randomly selects 6 mouse, and after fasting 12h, eyeball takes blood, by each heparin tube after the completion of blood sampling It is placed in 4 DEG C of environment and stands 12h, 10min Aspirate supernatants is then centrifuged with 3000r/min, then centrifuged using 1.5mL Pipe dispenses serum, is put into -20 DEG C of refrigerators and saves backup.Cervical dislocation is put to death, and splits abdominal cavity rapidly, and it is (empty to scrape off enteron aisle Intestines, ileum and colon) mixture chyme is placed in 1.5mL centrifuge tubes, 10 times of dilutions, (Escherichia coli and lactic acid are counted respectively Bacterium);(the limited public affairs of biotechnology share are controlled in raw north during kit is selected with kit measurement for glutamic-oxalacetic transaminease and glutamic-pyruvic transaminase Department).
The experiment packet of table 1
1st group Basal feed
2nd group + 50% mouldy maize flour of 50% basal feed
3rd group + 50% mouldy embodiment of maize flour+0.1% 1 of 50% basal feed
4th group + 50% mouldy embodiment of maize flour+0.1% 2 of 50% basal feed
5th group + 50% mouldy embodiment of maize flour+0.1% 3 of 50% basal feed
The present invention of table 2 is to mouse daily gain, gut flora, hepar damnification index table
Found out by table 2, several formulas of the invention can improve the daily gain of mouse, increase intestinal beneficial bacterium, suppress The growth of harmful bacteria, there is good protective effect to hepar damnification.

Claims (4)

1. one kind poultry poultry fodder takes off mould dose, it is characterized in that described composition includes:Glucose oxidase, yeast cell wall, tiltedly Ptilolite, probiotics;Probiotics viable bacteria is 3 × 10 in the composition7-3×1010Individual/gram, glucose oxidase accounts for gross mass 1%-30%, clinoptilolite is 0.5%-5%, surplus yeast cell wall.
2. a kind of poultry poultry fodder according to claim 1 takes off mould dose, it is characterized in that the probiotics is saccharomycete, withered grass Bacillus, lactic acid bacteria, form and are expressed as saccharomycete with the ratio between number of viable:Bacillus subtilis:Lactic acid bacteria=1:1:1.
3. a kind of poultry poultry fodder according to claim 1 or 2 takes off mould dose, it is characterized in that lactic acid bacteria is VREF, excrement Enterococcus, lactobacillus acidophilus, one kind of Lactobacillus plantarum or its combination.
4. a kind of poultry poultry fodder according to claim 1 takes off mould dose, it is characterised in that is to produce by the following method:
(1) according to 3 × 107-3×1010Cfu/g ratio weighs probiotics, is diluted according to the principle of doubling dilution with clinoptilolite Mix;
(2) yeast cell wall, glucose oxidase are added, mixes and produces.
CN201610742304.4A 2016-08-27 2016-08-27 One kind poultry poultry fodder takes off mould dose and preparation method thereof Pending CN107772195A (en)

Priority Applications (1)

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Application Number Priority Date Filing Date Title
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108850783A (en) * 2018-06-13 2018-11-23 西安默瑞柯生物科技有限公司 One boar food mycotoxin adsorption degradation agent and preparation method thereof
CN109221893A (en) * 2018-09-26 2019-01-18 山西大禹生物工程股份有限公司 Feed biology takes off mould dose and preparation method thereof
CN114145388A (en) * 2021-12-02 2022-03-08 黑龙江领康生物科技有限公司 Biological mildew removing agent for livestock feed and preparation method thereof
CN115067458A (en) * 2022-06-22 2022-09-20 浙江启润生物科技有限公司 Biodegradation method of mycotoxin in feed raw materials
CN115380995A (en) * 2022-07-25 2022-11-25 武汉新华扬生物股份有限公司 Bacterium enzyme acid composite preparation and application thereof

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108850783A (en) * 2018-06-13 2018-11-23 西安默瑞柯生物科技有限公司 One boar food mycotoxin adsorption degradation agent and preparation method thereof
CN109221893A (en) * 2018-09-26 2019-01-18 山西大禹生物工程股份有限公司 Feed biology takes off mould dose and preparation method thereof
CN114145388A (en) * 2021-12-02 2022-03-08 黑龙江领康生物科技有限公司 Biological mildew removing agent for livestock feed and preparation method thereof
CN115067458A (en) * 2022-06-22 2022-09-20 浙江启润生物科技有限公司 Biodegradation method of mycotoxin in feed raw materials
CN115380995A (en) * 2022-07-25 2022-11-25 武汉新华扬生物股份有限公司 Bacterium enzyme acid composite preparation and application thereof
CN115380995B (en) * 2022-07-25 2024-02-13 武汉新华扬生物股份有限公司 Bacterial enzyme acid composite preparation and application thereof

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