CN107760750A - A kind of method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material - Google Patents
A kind of method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material Download PDFInfo
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Abstract
The invention belongs to technical field of food biotechnology, and in particular to a kind of method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material.Pretreated corn protein powder solution is adjusted into pH value to faintly acid, then through hydrolysis process, supernatant is through drying as starch sugar product;Precipitation further hydrolysis, refined produce high F value oligopeptide product.Method disclosed by the invention has the advantages that technique is simple, equipment investment is few, product yield is high;The total protein content of high F value oligopeptide product is higher than 85%, and wherein dipeptides and the tripeptides proportion in total protein is more than 30 higher than 85%, F values, and good water solubility, color and luster flavor is good, can be widely applied to health food and nutraceutical field;Glucose content is higher than 50% in starch sugar product, good water solubility, can be widely applied to food and fermentation industry.
Description
Technical field
The invention belongs to technical field of food biotechnology, and in particular to a kind of synchronously to prepare high F using corn protein powder as raw material
It is worth the method for oligopeptides and starch sugar.
Background technology
The oligopeptides that high F value oligopeptide is made up of 2-9 amino acid residue, its be noteworthy characterized by branched-chain amino acid (leucine,
Isoleucine, valine) it is higher with the molar ratio of aromatic amino acid (tyrosine, phenylalanine, tryptophan) content, typically
More than 20.F values are 3.0-3.5 in normal human blood, and the F values with liver diseases patient blood only have 1.0 or lower.Study table
Bright, high F value oligopeptide has liver protecting, promotes the physiology work(such as alcohol metabolism, antifatigue, anti-oxidant and reduction serum cholesterol
Energy.Modern nutriology research finds that compared with macro-molecular protein, oligopeptides can be with carrier transport or cell bypass after ingesting
For the form of infiltration by absorbed intact, the absorption rate of dipeptides or tripeptides is significantly higher than the free amino acid of same composition, can be extensive
Applied to fields such as health food, nutraceutical and arsenics.
Corn starch sugar is using cornstarch as raw material, and the sugar produced, main bag are hydrolyzed through acid system, acid-enzyme hydrolysis method or two enzymes method
Include maltose and glucose.Starch sugar is the staple product of agriculture industrialization and corn deep processing, and the master of microbial fermentation
One of nutritional ingredient is wanted, can be widely applied to food and fermentation industry.Therefore, the corn starch sugar production way of low cost is found
Sound development of the footpath for Chinese national economy plays an important roll.
Corn protein powder is the Main By product of wet production starch process, and China's annual production is higher than 2,000,000 tons, wherein egg
White matter content is about 50-70%, and content of starch is about 20-30%.Zein is rich in leucine, isoleucine and valine etc.
Branched-chain amino acid, but lack the essential amino acids such as lysine, tryptophan, there is the uniqueness for being developed to food-borne high F value oligopeptide
Advantage, and maize oligopeptide is approved as new resource food by China.Therefore, using corn protein powder as raw material, exploitation synchronously obtains
The production technology for obtaining high F value oligopeptide and starch sugar is significant for food and functional food processing industry.
Domestic patent 201210136559.8 discloses a kind of preparation method of corn high-F-ratio-oligopebeverage beverage, using external source
Protease compound microorganism ferments method obtains corn high F value oligopeptide, but the patent only provides enzymolysis process, not to gained
The molecular weight and F values of oligopeptides are evaluated, can not clearly gained oligopeptides product performance.Domestic patent 201610015101.5
A kind of corn high F value oligopeptide production method with Soboring-up liver-protecting activity is disclosed, maize yellow-powder is through protease hydrolytic and activity
Carbon adsorption process obtains high F value oligopeptide, but three-level ultrafiltration and one-level nanofiltration process are contained in the technique, and technique is cumbersome, production
Product loss late and production cost are high, and the peptide chain composition of oligopeptides is not illustrated in the patent.At present, there is not yet with corn protein powder
For raw material, the relevant report of high F value oligopeptide and starch sugar is synchronously prepared.
The content of the invention
It is an object of the invention to provide a kind of side that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material
Method.
To achieve the above object, the present invention use technical scheme for:
A kind of method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material:By pretreated jade
Rice gluten powder solution adjusts pH value to faintly acid, then through hydrolysis process, supernatant is through drying as starch sugar product;Precipitate into one
One-step hydrolysis, refined produce high F value oligopeptide product.
Further:
1) pretreated corn protein powder solution is adjusted into pH value to faintly acid, an one-step hydrolysis is then carried out by hydrolase
Handle, separating and filtering collects supernatant and precipitation respectively after hydrolysis, and wherein supernatant is starch sugar product through drying;
2) above-mentioned acquisition precipitation is resuspended in water and obtains aqueous suspensions, regulation aqueous suspensions pH value is to alkalescent, then again by water
Enzyme hydrolysis is solved, separating and filtering collects supernatant after hydrolysis;
3) by above-mentioned steps 2) collect supernatant in add activated carbon reacted, realize an one-step refining, refine after point
It is stand-by from supernatant is collected by filtration;
4) by above-mentioned steps 3) collect supernatant through heating in vacuum concentration realize second step refinement treatment, then separated
Supernatant is collected in filter, and supernatant is through being drying to obtain high F value oligopeptide product.
In the step 1), the pretreatment of corn protein powder is mixes corn protein powder with deionized water, feed liquid quality
Volume ratio is 1:5-1:15, mixed liquor is incubated 0.5-2.0h under the conditions of 80-95 DEG C, is cooled to room temperature, obtains the jade of pretreatment
Rice gluten powder mixed liquor, as mixed liquor A.
In the step 1), by adding the saturated calcium hydroxide aqueous solution into mixed liquor A, regulation pH value to faintly acid,
Hydrolase A and hydrolase B are then added, constant temperature stirring, is reacted after terminating, heating termination enzyme digestion reaction, in separating and filtering collection
Clear liquid A and precipitate A;Wherein, supernatant A through drying, sterilize produce glucose content be higher than 50% starch sugar.
In the step 1), the pH value of mixed liquor A is adjusted to 5.0-6.0, then adds hydrolase A, hydrolase A and jade
The mass ratio of rice gluten powder is 0.5-2.0%, and reaction temperature is 55-75 DEG C, reaction time 0.5-6.0h;Then add water
The mass ratio of solution enzyme B, hydrolase B and corn protein powder is 0.5-2.0%, and reaction temperature is 40-60 DEG C, reaction time 0.5-
6.0h;Wherein, hydrolase A is alpha-amylase, and hydrolase B is α-Isosorbide-5-Nitrae-glucose hydrolysis enzyme.
In the step 2), deionized water mixing is added into above-mentioned acquisition precipitate A, feed liquid mass volume ratio is 1:5-1:
15, mixed liquid B is obtained, aqueous slkali is added into mixed liquid B, adjusts the pH value of mixed liquid B to alkalescent, and in backward mixed liquid B
Hydrolase C and hydrolase D is added, constant temperature stirring, after reaction terminates, heating termination enzyme digestion reaction, separating and filtering collects supernatant,
As supernatant B.
In the step 2), mixed liquid B adjusts pH value to 7.0-9.0 using the saturated calcium hydroxide aqueous solution, then adds
Hydrolase C and hydrolase D, hydrolase C are 4-8% with hydrolase D total amount and the mass ratio of raw material corn protein powder, reaction temperature
Spend for 40-60 DEG C, reaction time 12-24h, by supplementing the pH of saturated calcium hydroxide aqueous solution maintenance system in course of reaction
It is worth constant;Wherein, hydrolase C and corn protein powder mass ratio are 1-4%, and the mass ratio of hydrolase D and corn protein powder is 1-
4%;The hydrolase C is papain, trypsase, neutral proteinase, bromelain, compound protease and alkaline egg
One or more in white enzyme;Hydrolase D is the one or more in flavor protease, aminopeptidase and carboxypeptidase.
The one-step refining of step 3) one processing is that the supernatant B of collection is adjusted into pH value to 6.0-9.0, adds and lives after regulation
Property charcoal, activated carbon and supernatant B mass volume ratio be 3-5%, constant temperature stirring, after reaction terminates, separating and filtering collection supernatant,
As supernatant C;Wherein, activated carbon is food grade powder activated carbon.
An one-step refining is the supernatant B for collecting above-mentioned steps through 2M HCl solutions or saturation hydroxide in the step 3)
Calcium aqueous solution regulation pH value is 6.0-9.0, then adds activated carbon, constant temperature stirring, and reaction temperature is 10-60 DEG C, the reaction time
For 0.5-6.0h, separating and filtering collects supernatant, as supernatant C.
For the supernatant C for collecting above-mentioned steps in the step 4) under 0.02-0.08MPa vacuum condition, heating is dense
The solid content for being reduced to supernatant is 10-30%, and then separating and filtering collects supernatant D, realizes second step refinement treatment, adds
Hot temperature is 50-80 DEG C, and through being drying to obtain F values higher than 30, dipeptides and the tripeptides proportion in total protein are higher than supernatant D
85% high F value oligopeptide product.
The separating and filtering mode is the one or more in plate-frame filtering, centrifugal filtration or vacuum drum filtering;It is described
Drying mode is constant pressure and dry or is dried under reduced pressure.
A kind of methods described synchronously prepares high F value oligopeptide and starch sugar by raw material of corn protein powder, described to prepare
High F value oligopeptide or the application with starch sugar in health food, nutraceutical or arsenic is prepared.Wherein, the food
Different formulations acceptable at this stage can be prepared into.
Compared with the prior art the present invention has following distinguishing feature and good effect:
The abundant raw material that the present invention uses is easy to get, and protein content is high;Preparation method has that technique is simple, equipment investment
Less, non-environmental-pollution, the advantages that product yield is high, suitable for industrialized production.Gained high F value oligopeptide product has following feature:
Protein content is higher than 85%, and content of ashes is less than 8%, and product purity is high;F values are higher than 30, and dipeptides and tripeptides are in total protein
Proportion is higher than 85%, and bioactivity and intestinal absorption utilization rate are high, and good water solubility, color and luster flavor is good, can extensive use
In fields such as health food, nutraceutical and arsenics.Gained starch sugar product has following feature:Glucose content is high
In 50%, good water solubility, food and fermentation industry can be widely applied to.
The present invention is reclaimed by an one-step hydrolysis technique from corn protein powder obtains starch sugar, glucose in gained starch sugar
Content be higher than 50%, can be applied to food and fermentation industry;One aspect of the present invention can improve the comprehensive of raw material corn protein powder
Utilization ratio is closed, on the other hand can improve the protein content of high F value oligopeptide preparing raw material, and then improve products obtained therefrom high F value
The purity of oligopeptides, avoid desugar purge process numerous and diverse in conventional art.
Meanwhile zein can be effectively improved by using multienzyme composite hydrolysis technique in preparation process of the present invention
Hydrolysis efficiency, dipeptides and the tripeptides proportion in total protein are higher than 85% in gained high F value oligopeptide so that bioactivity and intestines
Road absorption rate is high, so as to avoid classification ultra-filtration process cumbersome in conventional art, saves production cost, shortens production week
Phase, improve product yield and product quality.
At the same time, using two unique one-step refining techniques in preparation process of the present invention, in the base of absorbent charcoal fine purification technique
On plinth, the concentration for the aromatic amino acid that dissociates in oligopeptide solution is improved by concentrating, makes free aromatic amino acid from solution
Separate out, remove free aromatic amino acid using filter separation method, further improve the F values of high F value oligopeptide product, reduce production
The mass fraction of free amino acid in product.
Brief description of the drawings
Fig. 1 is the canonical plotting that glucose content calculates in starch sugar provided in an embodiment of the present invention:Wherein, (A) is
The high-efficient liquid phase chromatogram of different concentration of glucose standard items;(B) it is glucose standard curve.
Fig. 2 is the liquid phase color provided in an embodiment of the present invention using main component in starch sugar product obtained by the inventive method
Spectrum identification diagram:Wherein, (A) is the gained starch sugar product of embodiment 1;(B) it is the gained starch sugar product of embodiment 2;(C) to be real
Apply the gained starch sugar product of example 3.
Fig. 3 is point provided in an embodiment of the present invention using high F value oligopeptide each component proportion obtained by the inventive method
Cloth situation map:Wherein, (A) is the gained high F value oligopeptide product of embodiment 1;(B) it is the gained high F value oligopeptide product of embodiment 2;(C)
For the gained high F value oligopeptide product of embodiment 3.
Embodiment
Explanation that the present invention will be further explained with reference to the accompanying drawings and examples.
By corn protein powder is preprocessed, an one-step hydrolysis and two one-step hydrolysis obtain starch sugar juice and oligopeptide solution, wherein,
One one-step hydrolysis process uses amylase and carbohydrase as hydrolase, and two one-step hydrolysis processes are complete using multiple protein enzyme composite hydrolysis
Into;Starch liquid sugar obtains starch sugar product through drying, sterilization, and oligopeptide solution is through the one-step refining of activated carbon one, the step of concentrating and separating two essence
System, dry, sterilization obtain high F value oligopeptide product.Which solve current high F value oligopeptide production technology route complexity, product yield
Low, the problem of production cost is high and corn protein powder utilization rate is low, realize the inexpensive, efficient of high F value oligopeptide and starch sugar
Prepared by rate, so as to make up the deficiencies in the prior art.
Embodiment 1
1) corn protein powder 10kg is taken, deionized water 80L is added into corn protein powder, is stirred in 90 DEG C of reactors
1.5h, room temperature is cooled to, produces mixed liquor A;
2) the saturated calcium hydroxide aqueous solution is added into mixed liquor A, the pH for adjusting mixed liquor is 5.0, is added into mixed liquor A
Enter alpha-amylase 100g, the constant temperature stirring reaction 6.0h in 55 DEG C of reactors, then add α-Isosorbide-5-Nitrae-grape into mixed liquor A again
Glycosylhydrolase 100g, the constant temperature stirring reaction 6.0h in 40 DEG C of reactors, after reaction terminates, it is incubated in 90 DEG C of reactors
0.5h, centrifugation, supernatant A and precipitate A is collected respectively;
3) supernatant A is identified through above-mentioned high performance liquid chromatography, and its main component is starch sugar (referring to Fig. 1 and 2 A);On
Clear liquid A produces starch sugar product after vacuum freeze drying, packaging, sterilization;
4) deionized water 80L is added into precipitate A, the saturated calcium hydroxide aqueous solution, regulation mixing are added into mixed liquor
The pH of liquid is 8.0, and bromelain 0.1kg, alkali protease 0.1kg and flavor protease 0.2kg are added into mixed liquor,
Stirring reaction 24h in 40 DEG C of reactors, after reaction terminates, 0.5h is incubated in 90 DEG C of reactors, centrifuged, collect supernatant B;
5) pH for adjusting supernatant B is 6.5, and active powdered carbon 2.4kg is added into supernatant B, is stirred in 30 DEG C of reactors
Reaction 0.5h is mixed, after reaction terminates, is separated using plate-frame filtering, collects supernatant C;
6) supernatant C is heated to concentration, vacuum 0.08MPa under vacuum, heating-up temperature is 50 DEG C, is concentrated into
Solid content is 30% in supernatant, produces supernatant D, is separated using centrifugal filtration, collects supernatant E;
7) supernatant E produces high F value oligopeptide product after vacuum freeze drying, packaging, sterilization.
The detection of above-mentioned acquisition high F value oligopeptide F values uses ultraviolet spectrophotometry, described in detail below:
In oligo peptide, branched-chain amino acid has the maximum absorption band of characteristic at 220nm, and aromatic amino acid exists
There is the maximum absorption band of characteristic at 280nm, and other amino acid are at this wavelength without absorption.Therefore, by high F value oligopeptide according to
The content of branched-chain amino acid and aromatic amino acid in high F value oligopeptide solution is estimated using ultraviolet specrophotometer, with OD values
Ratio estimates the size of F values.
High F value oligopeptide powder is taken, is configured to the 0.1mg/mL aqueous solution, after measured suction of the aqueous solution at 280nm wavelength
Light value is 0.031;After the oligopeptides aqueous solution is diluted into 10 times, light absorption value of the dilute solution at 220nm wavelength is 0.95 after measured;
Then, OD220 and OD280 ratio is 30.6.
The detection of gained high F value oligopeptide component uses high performance liquid chromatography-tandem mass method, described in detail below:
1) chromatographic condition
Chromatographic column is Waters Symmetry C18 (150mm × 2.1mm, 5 μm), and mobile phase A is that 0.1% formic acid is water-soluble
Liquid, Mobile phase B are 0.1% formic acid acetonitrile solution, and elution requirement is 0~40%B gradient elutions, elution time 30min, flow velocity
For 250 μ L/min, sample size is 5 μ L, and column temperature is 30 DEG C.
2) Mass Spectrometry Conditions
Ionization mode is electron spray, and spray voltage 4.5kV, capillary temperature is 350 DEG C, scanning of the mass spectrum scope (m/z)
For 50~1500, positive ion mode.
3) result calculates
Using the peptide sequence of each component in Mascot Distiller software analysis high F value oligopeptides.
By taking gained high F value oligopeptide sample in embodiment 1 as an example, after testing, peptide chain composition such as table 1 in high F value oligopeptide sample
It is shown:
The peptide chain composition situation of the high performance liquid chromatography-tandem mass of table 1 analysis high F value oligopeptide product
The detection of glucose content uses high performance liquid chromatography in gained starch sugar, described in detail below:
1) chromatographic condition
Chromatographic column is Bio-Rad Aminex HPX87H (300mm × 7.8mm, 9 μm), and mobile phase is 5mmol/L H2SO4
Solution, flow velocity 0.6mL/min, detector are differential refraction detector, and standard liquid is the glucose solution of various concentrations
(0.5~2.5mg/mL), sample size are 10 μ L, and column temperature is 60 DEG C.
2) result calculates
The appearance time of glucose is recorded, the concentration gradient of establishing criteria glucose solution peak area corresponding with its, is painted
Glucose standard curve processed (referring to Fig. 1 and Fig. 2).According to the appearance time of each component in sample, labelled glucose component, foundation
Standard curve, calculate the content of glucose in sample.
After testing, the yield of high F value oligopeptide is 29% in the present embodiment, protein content 87%, and content of ashes is
6.5%, through above-mentioned ultraviolet spectrophotometry detect F values be 30.6, detected through above-mentioned high performance liquid chromatography-tandem mass method
The content of dipeptides and tripeptides is respectively 79.4% and 14.2% (referring to Fig. 3 A) in total protein;The yield of starch sugar is 15%, its
Middle glucose content is 60%.
Embodiment 2
1) corn protein powder 10kg is taken, deionized water 50L is added into corn protein powder, is stirred in 80 DEG C of reactors
2.0h, room temperature is cooled to, produces mixed liquor A;
2) the saturated calcium hydroxide aqueous solution is added into mixed liquor A, the pH for adjusting mixed liquor is 6.0, is added into mixed liquor A
Enter alpha-amylase 50g, the constant temperature stirring reaction 3.0h in 65 DEG C of reactors, then add α-Isosorbide-5-Nitrae-grape into mixed liquor A again
Glycosylhydrolase 50g, the constant temperature stirring reaction 0.5h in 45 DEG C of reactors, after reaction terminates, 0.5h is incubated in 90 DEG C of reactors,
Centrifugation, supernatant A and precipitate A are collected respectively;
3) supernatant A is identified through above-mentioned high performance liquid chromatography, and its main component is starch sugar (referring to Fig. 1 and 2 B);On
After clear liquid A spray-dried (EAT is 180 DEG C, and leaving air temp is 90 DEG C), packaging, sterilization, starch sugar product is produced;
4) deionized water 50L is added into precipitate A, the saturated calcium hydroxide aqueous solution, regulation mixing are added into mixed liquor
The pH of liquid is 7.0, and papain 0.2kg, neutral proteinase 0.2kg and flavor protease 0.1kg are added into mixed liquor,
Stirring reaction 20h in 50 DEG C of reactors, after reaction terminates, 0.5h is incubated in 90 DEG C of reactors, centrifuged, collect supernatant B;
5) pH value for adjusting supernatant B is 3.0, active powdered carbon 2.5kg is added into supernatant B, in 10 DEG C of reactors
Stirring reaction 3h, after reaction terminates, separated using plate-frame filtering, collect supernatant C;
6) supernatant C is heated to concentration, vacuum 0.06MPa under vacuum, heating-up temperature is 65 DEG C, is concentrated into
Solid content is 20% in supernatant, produces supernatant D, is separated using plate-frame filtering, collects supernatant E;
7) after supernatant E spray-dried (EAT is 190 DEG C, and leaving air temp is 95 DEG C), packaging, sterilization, produce
High F value oligopeptide product.
After testing, the yield of high F value oligopeptide is 25% in the present embodiment, protein content 89%, and content of ashes is
6.8%, through above-mentioned ultraviolet spectrophotometry detect F values be 34, detect always through above-mentioned high performance liquid chromatography-tandem mass method
The content of dipeptides and tripeptides is respectively 72.9% and 20.6% (referring to Fig. 3 B) in albumen;The yield of starch sugar is 17%, wherein
Glucose content is 55%.
Embodiment 3
1) corn protein powder 10kg is taken, deionized water 150L is added into corn protein powder, is stirred in 95 DEG C of reactors
0.5h, room temperature is cooled to, produces mixed liquor A;
2) the saturated calcium hydroxide aqueous solution is added into mixed liquor A, the pH for adjusting mixed liquor is 5.5, is added into mixed liquor A
Enter alpha-amylase 200g, the constant temperature stirring reaction 0.5h in 75 DEG C of reactors, then add α-Isosorbide-5-Nitrae-grape into mixed liquor A again
Glycosylhydrolase 200g, the constant temperature stirring reaction 3.0h in 60 DEG C of reactors, after reaction terminates, it is incubated in 90 DEG C of reactors
0.5h, centrifugation, supernatant A and precipitate A is collected respectively;
3) supernatant A is identified through above-mentioned high performance liquid chromatography, and its main component is starch sugar (referring to Fig. 1 and 2 C);On
Clear liquid A produces starch sugar product after spray drying (EAT is 195 DEG C, and leaving air temp is 80 DEG C), packaging, sterilization;
4) deionized water 150L is added into precipitate A, the saturated calcium hydroxide aqueous solution, regulation mixing are added into mixed liquor
The pH of liquid is 9.0, trypsase 0.1kg, compound protease 0.3kg and flavor protease 0.4kg is added into mixed liquor, 60
Stirring reaction 12h in DEG C reactor, after reaction terminates, 0.5h is incubated in 90 DEG C of reactors, centrifuged, collect supernatant B;
5) pH value for adjusting supernatant B is 9.0, and active powdered carbon 6kg is added into supernatant B, is stirred in 60 DEG C of reactors
Reaction 6h is mixed, after reaction terminates, is separated using plate-frame filtering, collects supernatant C;
6) supernatant C is heated to concentration, vacuum 0.02MPa under vacuum, heating-up temperature is 80 DEG C, is concentrated into
Solid content is 10% in supernatant, produces supernatant D, is separated by filtration using vacuum drum, collects supernatant E;
7) after supernatant E spray-dried (EAT is 180 DEG C, and leaving air temp is 80 DEG C), packaging, sterilization, produce
High F value oligopeptide product.
After testing, the yield of high F value oligopeptide is 31% in the present embodiment, protein content 87%, and content of ashes is
6.7%, through above-mentioned ultraviolet spectrophotometry detect F values be 31, detect always through above-mentioned high performance liquid chromatography-tandem mass method
The content of dipeptides and tripeptides is respectively 73.9% and 19.5% (referring to Fig. 3 C) in albumen;The yield of starch sugar is 16%, wherein
Glucose content is 58%.
Embodiment 4
The present invention obtains high F value oligopeptide and can be widely applied to the fields such as health food, nutraceutical or arsenic,
Exemplified by specially using above-described embodiment acquisition product preparation Functional Polypeptides lozenge:
The high F value oligopeptide powder 20g obtained by above-mentioned any one embodiment is taken, adds deionized water 8mL, is mixed;Again thereto
Cyclodextrin 20g is added, fully after dissolving;Dextrin 15g, malic acid 3.5g, starch 21.5g, vitamin C are sequentially added thereto
1g, xylitol 10g, homogeneous;Add magnesium stearate 1g thereto again, homogeneous, sieve, dry, tabletting.
In the present embodiment, the quality of every lozenge is about 800mg, and wherein the content of high F value oligopeptide is 20%.
Embodiment 5
Starch sugar can be widely applied to food or fermentation industry in the present invention, specially obtain product using above-described embodiment
The schizochytrium limacinum that ferments is produced exemplified by DHA:
Preliminary fermentation culture medium is prepared by carbon source of glucose, medium component is as follows:Glucose 120g/L, dusty yeast
10g/L, ammonium acetate 1.5g/L, sea crystal 10g/L, potassium dihydrogen phosphate 4g/L, bitter salt 4.6g/L, calcium chloride 3.5g/
L, vitamin B120.005g/L;10% strain is seeded to fermentation medium, after the 30h that ferments, with 1.8g/L/h feed rate
Add the starch sugar juice in the present invention;Ferment after 100h, the starch sugar in the present invention is added with 1.2g/L/h feed rate
Solution;Ferment after 120h, harvest thalline.
In the present embodiment, the biomass of schizochytrium limacinum is 70g/L, and DHA yield is 20g/L.
Claims (10)
- A kind of 1. method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material, it is characterised in that:Will be through pre- The corn protein powder solution of processing adjusts pH value to faintly acid, then through hydrolysis process, supernatant is through drying as starch sugar product; Precipitation further hydrolysis, refined produce high F value oligopeptide product.
- 2. the method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material as described in claim 1, it is special Sign is:1) pretreated corn protein powder solution is adjusted into pH value to faintly acid, an one-step hydrolysis is then carried out by hydrolase Manage, separating and filtering collects supernatant and precipitation respectively after hydrolysis, and wherein supernatant is starch sugar product through drying;2) above-mentioned acquisition precipitation is resuspended in water and obtains aqueous suspensions, regulation aqueous suspensions pH value is to alkalescent, then again by hydrolase Hydrolyze, separating and filtering collects supernatant after hydrolysis;3) by above-mentioned steps 2) activated carbon is added in the supernatant collected is reacted, an one-step refining is realized, was separated after refined Supernatant is collected in filter, stand-by;4) by above-mentioned steps 3) collect supernatant through heating in vacuum concentration realize second step refinement treatment, then separating and filtering receive Collect supernatant, supernatant is through being drying to obtain high F value oligopeptide product.
- 3. the method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material as described in claim 2, it is special Sign is:The pretreatment of the step 1) corn protein powder is mixes corn protein powder with deionized water, feed liquid quality volume Than for 1:5-1:15, mixed liquor is incubated 0.5-2.0h under the conditions of 80-95 DEG C, is cooled to room temperature, obtains the corn egg of pretreatment White powder mixed liquor, as mixed liquor A.
- 4. the method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material as described in claim 3, it is special Sign is:In the step 1), by adding the saturated calcium hydroxide aqueous solution into mixed liquor A, pH value is adjusted to faintly acid, and Hydrolase A and hydrolase B are added afterwards, and constant temperature stirring, after reaction terminates, heating termination enzyme digestion reaction, separating and filtering collects supernatant Liquid A and precipitate A;Wherein, supernatant A through drying, sterilize produce glucose content be higher than 50% starch sugar.
- 5. the method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material as described in claim 4, it is special Sign is:In the step 1), the pH value of mixed liquor A is adjusted to 5.0-6.0, then adds hydrolase A, hydrolase A and jade The mass ratio of rice gluten powder is 0.5-2.0%, and reaction temperature is 55-75 DEG C, reaction time 0.5-6.0h;Then add water The mass ratio of solution enzyme B, hydrolase B and corn protein powder is 0.5-2.0%, and reaction temperature is 40-60 DEG C, reaction time 0.5- 6.0h;Wherein hydrolase A is alpha-amylase, and hydrolase B is α-Isosorbide-5-Nitrae-glucose hydrolysis enzyme.
- 6. the method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material as described in claim 2, it is special Sign is:In the step 2), deionized water mixing is added into above-mentioned acquisition precipitate A, feed liquid mass volume ratio is 1:5-1: 15, mixed liquid B is obtained, aqueous slkali is added into mixed liquid B, adjusts the pH value of mixed liquid B to alkalescent, and in backward mixed liquid B Hydrolase C and hydrolase D is added, constant temperature stirring, after reaction terminates, heating termination enzyme digestion reaction, separating and filtering collects supernatant, As supernatant B.
- 7. the method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material as described in claim 6, it is special Sign is:In the step 2), mixed liquid B adjusts pH value to 7.0-9.0 using the saturated calcium hydroxide aqueous solution, then adds water The mass ratio for solving enzyme C and hydrolase D, hydrolase C and hydrolase D total amount and raw material corn protein powder is 4-8%, reaction temperature For 40-60 DEG C, reaction time 12-24h, by supplementing the pH value of saturated calcium hydroxide aqueous solution maintenance system in course of reaction It is constant;Wherein, hydrolase C and corn protein powder mass ratio are 1-4%, and the mass ratio of hydrolase D and corn protein powder is 1- 4%;The hydrolase C is papain, trypsase, neutral proteinase, bromelain, compound protease and alkaline egg One or more in white enzyme;Hydrolase D is the one or more in flavor protease, aminopeptidase and carboxypeptidase.
- 8. the method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material as described in claim 2, it is special Sign is:The one-step refining of step 3) one processing is that the supernatant B of collection is adjusted into pH value to 6.0-9.0, adds and lives after regulation Property charcoal, activated carbon and supernatant B mass volume ratio be 3-5%, constant temperature stirring, after reaction terminates, separating and filtering collection supernatant Liquid, as supernatant C;Wherein, activated carbon is food grade powder activated carbon.
- 9. the method that high F value oligopeptide and starch sugar are synchronously prepared using corn protein powder as raw material as described in claim 2, it is special Sign is:The supernatant C for collecting above-mentioned steps in the step 4) is under 0.02-0.08MPa vacuum condition, heating concentration Solid content to supernatant is 10-30%, and then separating and filtering collects supernatant D, realizes second step refinement treatment, is heated Temperature is 50-80 DEG C, supernatant D through drying, sterilize and produce F values higher than 30, dipeptides and tripeptides the proportion height in total protein In 85% high F value oligopeptide product.
- 10. a kind of method described in claim 1 synchronously prepares high F value oligopeptide and starch sugar by raw material of corn protein powder, its It is characterised by:It is described prepare high F value oligopeptide or with starch sugar in health food, nutraceutical or arsenic is prepared Application.
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CN108624643A (en) * | 2018-04-02 | 2018-10-09 | 中国科学院青岛生物能源与过程研究所 | The method for preparing high added value oligopeptides as raw material using micro- quasi- ball algae-residue |
CN108642113A (en) * | 2018-05-03 | 2018-10-12 | 江南大学 | A method of efficiently and stabilization prepares high F value corn oligopeptide |
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CN108642113A (en) * | 2018-05-03 | 2018-10-12 | 江南大学 | A method of efficiently and stabilization prepares high F value corn oligopeptide |
CN108642113B (en) * | 2018-05-03 | 2021-06-25 | 江南大学 | Method for efficiently and stably preparing high-F-value corn oligopeptide |
WO2020014997A1 (en) * | 2018-07-20 | 2020-01-23 | 江南大学 | High f-ratio oligopeptide and preparation method therefor |
CN110878291A (en) * | 2019-12-09 | 2020-03-13 | 北京工商大学 | High-yield specific functional oligopeptide enzyme derived from aspergillus niger and engineering bacterium thereof |
CN110878291B (en) * | 2019-12-09 | 2020-05-26 | 北京工商大学 | High-yield specific functional oligopeptide enzyme derived from aspergillus niger and engineering bacterium thereof |
CN114451562A (en) * | 2022-03-04 | 2022-05-10 | 广东绿宝堂健康科技有限公司 | Hovenia dulcis, radix puerariae and corn oligopeptide composition, beverage and preparation method thereof |
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