CN107751607A - A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea - Google Patents
A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea Download PDFInfo
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Abstract
The invention provides a kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea, belong to feed additive field.Additive of the present invention is made up of by weight following raw material：68 parts of oyster shell whiting, 7 12 parts of tea extract, 8 12 parts of lemon dregs extract, 10 14 parts of Fructus Crataegi fermented product, 57 parts of crab shell powder, 12 16 parts of montmorillonite.It by scientific matching, can effectively suppress the growth of mould and the generation of mycotoxin in feed, improve feed palatability, meanwhile, pig intestinal immunity is effectively improved, promotes pig intestinal growth, prevents and treats the generation of pig epidemic diarrhea.Used raw material is green safe additive raw material, be can be widely applied in live pig daily ration, improves culture benefit.
Description
Technical field：The present invention relates to boar mold-proof oxidation-resistant agent, more particularly to one kind to have preventing and treating pig popular
The mold-proof oxidation-resistant agent of diarrhoea.
Background technology：
Feed production, processing, transport, storage and using etc. during inevitably remain, be mixed into or produce mould and mould
Verticillium toxin, feed mold problem turn into Feed Manufacturing it is noted that one of subject matter.Going mouldy reduces the nutriture value of feed
Value, influences palatability, more seriously causes feed product mycotoxin exceeded, suppresses breeding performonce fo animals, reduces animal
Immunity of organisms, it might even be possible to which health is threatened by food chain.
Pig epidemic diarrhea is a kind of contact enteric infectious disease as caused by Porcine epidemic diarrhea virus, it is characterized in that vomitting
Tell, suffer from diarrhoea, be dehydrated, all can infection morbidity in the pig of each growth phase.The virus is present in chitling road villus epithelial cells
And lymphonodi mesenterici, after being discharged with excrement, pollute environment, feed, drinking-water, the vehicles and apparatus etc. and infect, main sense
Dye approach is alimentary canal.
Mold-proof oxidation-resistant agent in the market is mostly the simple composite of mould inhibitor and antioxidant, and using chemical composition as
It is main, residue problem is also easy to produce, some can also have special odor to influence feed palatability.
By using medicine come to treat pig epidemic diarrhea disease be conventional means, this can alleviate disease to a certain extent
Disease, but can not fundamentally solve, and long-term use of medicine can cause to remain, and produce food-safety problem, while can also promote
Virus produces drug resistance, is unfavorable for the long-range high-efficient development of aquaculture.
There is presently no a kind of pig epidemic diarrhea and can that can prevent and treat effectively to prevent the feed addictive of feed mold, because
This, develops a kind of mold-proof oxidation-resistant agent that can effectively prevent and treat pig epidemic diarrhea, has important meaning for improving culture benefit
Justice.
The content of the invention：
The present invention declines for feed nutritive value caused by feed mold, palatability is poor, and the problems such as pig epidemic diarrhea,
A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea is provided, for preventing and treating pig epidemic diarrhea and preventing that feed is mould
Become, improve culture benefit.
The purpose of the present invention is realized by following technical scheme：
A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea, it is characterised in that be made up of by weight following raw material：
Oyster shell whiting 6-8 parts, tea extract 7-12 parts, lemon dregs extract 8-12 parts, Fructus Crataegi fermented product 10-14 parts, crab shell powder 5-7
Part, montmorillonite 12-16 parts.
The preparation method of the green-tea extract：Take tealeaves to crushed 80-100 mesh sieves, obtain tea leaf powder, add tealeaves silty
The distilled water of 5-7 times of amount, stirs, and is heated to 35-40 DEG C, adds tea leaf powder quality 0.05-0.08% complex enzyme, stirring
30-50min, absolute ethyl alcohol is added afterwards, be 40-50% to concentration of alcohol, extracted 1-2h, filtering, obtain filtrate, carry out vacuum and do
It is dry, obtain tea extract；
The complex enzyme is by cellulase, pectase, protease in mass ratio 1:1-2:1-2 is formed；
The preparation method of the lemon dregs extract；Fresh Lemon slag is taken, IQF 30-40min under the conditions of being placed in -30 DEG C,
After crushing, it is placed in container and adds the water of 4-6 times of lemon dregs weight, heat 70-80 DEG C of keeping temperature, maintain 2-4h, afterwards will
Temperature is reduced to 37-42 DEG C, adds mixed enzyme, and mixed enzyme is pressed by 'beta '-mannase, pectase, zytase, cellulase
Weight is than 1:1-2:1.0-1.4:0.8-1.0 is formed, and the addition of mixed enzyme is the 0.1-0.14% of lemon dregs weight, and PH is adjusted
For 6-6.5, hydrolysis temperature is 37-42 DEG C, enzymolysis time 3-4h, is filtered after enzymolysis, obtains filtrate, concentrate the filtrate to 1/4-1/3
Volume, concentrate is obtained, add the absolute ethyl alcohol of 1.0-1.8 times of concentrate, after standing 20-30min, centrifuging and taking lower sediment is simultaneously
It is dried in vacuo, obtains lemon dregs extract.
The preparation method of the Fructus Crataegi fermented product：Hawthorn is taken, crushed 60-80 mesh sieves, hawthorn powder is obtained and is placed in container, add
Enter distilled water, the water content of hawthorn powder is reached 20-34%, add the gemma bacillus agent of mixture weight 0.05%, be sealed by fermentation
36-48h, low-temperature air-drying is carried out after fermentation ends, obtains Fructus Crataegi fermented product；
The preparation method of the gemma bacillus agent：Transferred from inclined-plane and cultivate bacillus, the seed liquor switching after spreading cultivation step by step
Enter in fermentation tank, it is 30-35 DEG C to control temperature, aerlbic culture 48-60 hours, 90 revs/min of mixing speed, ventilation（V/V）1:
0.5, tank pressure 0.06MPa, fermentation finishes, and gemma bacteria agent is made in spray drying；
The slant medium percentage by weight forms：Potato juice 20%, glucose 2%, agar 2.0%, herba houttuyniae powder
0.8%, setose thistle powder 1.2%, remaining is pure water；
Spread cultivation step by step first order seed, secondary seed, three-level seed, first class seed pot, secondary seed tank and the fermentation life used
Production used medium percentage by weight, which forms, is：Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, lemon ground-slag 0.8%, tea leaf powder 1.2%, bark of eucommia powder 1.8%, remaining is pure water, pH 6.5.
Product preparation method of the present invention is as follows：
Oyster shell whiting, tea extract, lemon dregs extract, Fructus Crataegi fermented product, crab shell powder, montmorillonite are mixed and produced；
Addition of the present invention in swine rationses is 800-1200g/ tons.
Beneficial effect：
It is Tea Polyphenols, caffeine, lipopolysaccharides, theanine etc. that tealeaves, which has the main component of pharmacological action, has anti-aging, suppression disease
Disease, antibiosis and antiviral functions, tealeaves can reduce alcohol extracting concentration of alcohol after enzymolysis processing, reduce ethanol and use, and extraction efficiency
Greatly improve；Lemon dregs are accessory substance of the lemon after squeezing the juice, and contain citric acid, malic acid, aurantiamarin, aurantiin, coumarin
Deng composition, there is anti-inflammation, anti-aging, prevention of cardiovascular disease effect, the lemon dregs extract obtained after extracted is lived
Property composition greatly improves；Hawthorn, also known as hawthorn, large-fruited Chinese hawthorn, rose family May, contain organic acid, tartaric acid, crataegolic acid, Chinese holly
Rafter acid, Vitexin isoreactivity composition, there is aid digestion, inhibiting bacteria and diminishing inflammation, promote animal body specificity and nospecific immunity work(
Can and other effects, in preparation process, the gemma bacillus agent fermentation efficiency through processing greatly improves Fructus Crataegi fermented product, and is preparing
During absorb the active components of the Chinese herbal medicines such as cordate houttuynia, setose thistle, be greatly improved the action effect of Fructus Crataegi fermented product.This hair
It is bright by tea extract, lemon dregs extract, Fructus Crataegi fermented product and other components, by scientific matching, can effectively suppress feed
The growth of middle mould and the generation of mycotoxin, improve feed palatability, meanwhile, pig intestinal immunity is effectively improved, is promoted
Pig intestinal growth, prevent and treat the generation of pig epidemic diarrhea.Additive of the present invention has been surprisingly found that the additive pair during invention
The streptococcosis of pig has good prevention effect.
Embodiment
Embodiment 1
A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea, it is characterised in that be made up of by weight following raw material：
6 parts of oyster shell whiting, 7 parts of tea extract, 8 parts of lemon dregs extract, 10 parts of Fructus Crataegi fermented product, 5 parts of crab shell powder, 12 parts of montmorillonite.
The preparation method of the green-tea extract：Take tealeaves to crushed 80 mesh sieves, obtain tea leaf powder, add tea leaf powder quality 5
Distilled water again, stirs, and is heated to 35 DEG C, adds the complex enzyme of tea leaf powder quality 0.05%, stirs 30min, adds afterwards
Absolute ethyl alcohol, it is 40% to concentration of alcohol, extracts 1h, filtering, obtain filtrate, be dried in vacuo, obtain tea extract；
The complex enzyme is by cellulase, pectase, protease in mass ratio 1:1:1 composition；
The preparation method of the lemon dregs extract；Fresh Lemon slag is taken, IQF 30min under the conditions of being placed in -30 DEG C, is crushed
Afterwards, it is placed in container and adds the water of 4 times of lemon dregs weight, heat 70 DEG C of keeping temperature, maintains 2h, temperature is reduced to 37 afterwards
DEG C, mixed enzyme is added, mixed enzyme is by 'beta '-mannase, pectase, zytase, cellulase by weight 1:1:1.0:
0.8 composition, the addition of mixed enzyme are adjusted to 6 for 0.1%, PH of lemon dregs weight, and hydrolysis temperature is 37 DEG C, enzymolysis time 3h,
Filtered after enzymolysis, obtain filtrate, concentrated the filtrate to 1/4 volume, obtain concentrate, add the absolute ethyl alcohol of 1.0 times of concentrates, it is quiet
After putting 20min, centrifuging and taking lower sediment is simultaneously dried in vacuo, and obtains lemon dregs extract.
The preparation method of the Fructus Crataegi fermented product：Hawthorn is taken, crushed 60 mesh sieves, hawthorn powder is obtained and is placed in container, is added
Distilled water, the water content of hawthorn powder is set to add the gemma bacillus agent of mixture weight 0.05% up to 20%, be sealed by fermentation 36h, hair
Ferment carries out low-temperature air-drying after terminating, and obtains Fructus Crataegi fermented product；
The preparation method of the gemma bacillus agent：Transferred from inclined-plane and cultivate bacillus, the seed liquor switching after spreading cultivation step by step
Enter in fermentation tank, it is 30 DEG C to control temperature, aerlbic culture 48 hours, 90 revs/min of mixing speed, ventilation（V/V）1:0.5, tank
0.06MPa is pressed, fermentation finishes, and gemma bacteria agent is made in spray drying；
The slant medium percentage by weight forms：Potato juice 20%, glucose 2%, agar 2.0%, herba houttuyniae powder
0.8%, setose thistle powder 1.2%, remaining is pure water；
Spread cultivation step by step first order seed, secondary seed, three-level seed, first class seed pot, secondary seed tank and the fermentation life used
Production used medium percentage by weight, which forms, is：Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, lemon ground-slag 0.8%, tea leaf powder 1.2%, bark of eucommia powder 1.8%, remaining is pure water, pH 6.5.
Product preparation method of the present invention is as follows：
Oyster shell whiting, tea extract, lemon dregs extract, Fructus Crataegi fermented product, crab shell powder, montmorillonite are mixed and produced；
Addition of the present invention in swine rationses is 1200g/ tons.
Embodiment 2
A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea, it is characterised in that be made up of by weight following raw material：
7 parts of oyster shell whiting, 10 parts of tea extract, 10 parts of lemon dregs extract, 12 parts of Fructus Crataegi fermented product, 6 parts of crab shell powder, montmorillonite 14
Part.
The preparation method of the green-tea extract：Take tealeaves to crushed 90 mesh sieves, obtain tea leaf powder, add tea leaf powder quality 6
Distilled water again, stirs, and is heated to 37 DEG C, adds the complex enzyme of tea leaf powder quality 0.06%, stirs 40min, adds afterwards
Absolute ethyl alcohol, it is 45% to concentration of alcohol, extracts 1.5h, filtering, obtain filtrate, be dried in vacuo, obtain tea extract；
The complex enzyme is by cellulase, pectase, protease in mass ratio 1:1.5:1.5 composition；
The preparation method of the lemon dregs extract；Fresh Lemon slag is taken, IQF 35min under the conditions of being placed in -30 DEG C, is crushed
Afterwards, it is placed in container and adds the water of 5 times of lemon dregs weight, heat 75 DEG C of keeping temperature, maintains 3h, temperature is reduced to 40 afterwards
DEG C, mixed enzyme is added, mixed enzyme is by 'beta '-mannase, pectase, zytase, cellulase by weight 1:1.5:1.2:
0.9 composition, the addition of mixed enzyme are adjusted to 6.3 for 0.12%, PH of lemon dregs weight, and hydrolysis temperature is 40 DEG C, enzymolysis time
3.5h, filter after enzymolysis, obtain filtrate, concentrate the filtrate to 1/3 volume, obtain concentrate, add the anhydrous second of 1.4 times of concentrates
Alcohol, after standing 205min, centrifuging and taking lower sediment is simultaneously dried in vacuo, and obtains lemon dregs extract.
The preparation method of the Fructus Crataegi fermented product：Hawthorn is taken, crushed 70 mesh sieves, hawthorn powder is obtained and is placed in container, is added
Distilled water, the water content of hawthorn powder is set to add the gemma bacillus agent of mixture weight 0.05% up to 27%, be sealed by fermentation 42h, hair
Ferment carries out low-temperature air-drying after terminating, and obtains Fructus Crataegi fermented product；
The preparation method of the gemma bacillus agent：Transferred from inclined-plane and cultivate bacillus, the seed liquor switching after spreading cultivation step by step
Enter in fermentation tank, it is 33 DEG C to control temperature, aerlbic culture 54 hours, 90 revs/min of mixing speed, ventilation（V/V）1:0.5, tank
0.06MPa is pressed, fermentation finishes, and gemma bacteria agent is made in spray drying；
The slant medium percentage by weight forms：Potato juice 20%, glucose 2%, agar 2.0%, herba houttuyniae powder
0.8%, setose thistle powder 1.2%, remaining is pure water；
Spread cultivation step by step first order seed, secondary seed, three-level seed, first class seed pot, secondary seed tank and the fermentation life used
Production used medium percentage by weight, which forms, is：Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, lemon ground-slag 0.8%, tea leaf powder 1.2%, bark of eucommia powder 1.8%, remaining is pure water, pH 6.5.
Product preparation method of the present invention is as follows：
Oyster shell whiting, tea extract, lemon dregs extract, Fructus Crataegi fermented product, crab shell powder, montmorillonite are mixed and produced；
Addition of the present invention in swine rationses is 1000g/ tons.
Embodiment 3
A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea, it is characterised in that be made up of by weight following raw material：
8 parts of oyster shell whiting, 12 parts of tea extract, 12 parts of lemon dregs extract, 14 parts of Fructus Crataegi fermented product, 7 parts of crab shell powder, montmorillonite 16
Part.
The preparation method of the green-tea extract：Take tealeaves to crushed 100 mesh sieves, obtain tea leaf powder, add tea leaf powder quality 7
Distilled water again, stirs, and is heated to 40 DEG C, adds the complex enzyme of tea leaf powder quality 0.08%, stirs 50min, adds afterwards
Absolute ethyl alcohol, it is 50% to concentration of alcohol, extracts 2h, filtering, obtain filtrate, be dried in vacuo, obtain tea extract；
The complex enzyme is by cellulase, pectase, protease in mass ratio 1:2:2 compositions；
The preparation method of the lemon dregs extract；Fresh Lemon slag is taken, IQF 40min under the conditions of being placed in -30 DEG C, is crushed
Afterwards, it is placed in container and adds the water of 4-6 times of lemon dregs weight, heat 80 DEG C of keeping temperature, maintains 4h, be afterwards reduced to temperature
42 DEG C, mixed enzyme is added, mixed enzyme is by 'beta '-mannase, pectase, zytase, cellulase by weight 1: 2:
1.4:1.0 compositions, the addition of mixed enzyme are adjusted to 6.5 for 0.14%, PH of lemon dregs weight, and hydrolysis temperature is 42 DEG C, enzyme
Time 4h is solved, is filtered after enzymolysis, is obtained filtrate, concentrate the filtrate to 1/3 volume, obtain concentrate, add the nothing of 1.8 times of concentrates
Water-ethanol, after standing 30min, centrifuging and taking lower sediment is simultaneously dried in vacuo, and obtains lemon dregs extract.
The preparation method of the Fructus Crataegi fermented product：Hawthorn is taken, crushed 80 mesh sieves, hawthorn powder is obtained and is placed in container, is added
Distilled water, the water content of hawthorn powder is set to add the gemma bacillus agent of mixture weight 0.05% up to 34%, be sealed by fermentation 48h, hair
Ferment carries out low-temperature air-drying after terminating, and obtains Fructus Crataegi fermented product；
The preparation method of the gemma bacillus agent：Transferred from inclined-plane and cultivate bacillus, the seed liquor switching after spreading cultivation step by step
Enter in fermentation tank, it is 35 DEG C to control temperature, aerlbic culture 60 hours, 90 revs/min of mixing speed, ventilation（V/V）1:0.5, tank
0.06MPa is pressed, fermentation finishes, and gemma bacteria agent is made in spray drying；
The slant medium percentage by weight forms：Potato juice 20%, glucose 2%, agar 2.0%, herba houttuyniae powder
0.8%, setose thistle powder 1.2%, remaining is pure water；
Spread cultivation step by step first order seed, secondary seed, three-level seed, first class seed pot, secondary seed tank and the fermentation life used
Production used medium percentage by weight, which forms, is：Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, lemon ground-slag 0.8%, tea leaf powder 1.2%, bark of eucommia powder 1.8%, remaining is pure water, pH 6.5.
Product preparation method of the present invention is as follows：
Oyster shell whiting, tea extract, lemon dregs extract, Fructus Crataegi fermented product, crab shell powder, montmorillonite are mixed and produced；
Addition of the present invention in swine rationses is 800g/ tons.
Embodiment 4
A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea, it is characterised in that be made up of by weight following raw material：
10 parts of oyster shell whiting, 5 parts of tea extract, 6 parts of lemon dregs extract, 18 parts of Fructus Crataegi fermented product, 9 parts of crab shell powder, 10 parts of montmorillonite.
Other are the same as embodiment 2.
Embodiment 5
A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea, it is characterised in that be made up of by weight following raw material：
7 parts of oyster shell whiting, 10 parts of tea extract, 10 parts of lemon dregs extract, 12 parts of Fructus Crataegi fermented product, 6 parts of crab shell powder, montmorillonite 14
Part.
The preparation method of the lemon dregs extract；Fresh Lemon slag is taken, IQF 50min under the conditions of being placed in -30 DEG C,
After crushing, it is placed in container and adds the water of 7 times of lemon dregs weight, heat 60 DEG C of keeping temperature, maintain 5h, afterwards reduce temperature
To 35 DEG C, mixed enzyme is added, mixed enzyme is by 'beta '-mannase, pectase, zytase, cellulase by weight 1:3:
1.5:0.6 composition, the addition of mixed enzyme are adjusted to 7 for 0.15%, PH of lemon dregs weight, and hydrolysis temperature is 35 DEG C, during enzymolysis
Between 5h, filter after enzymolysis, obtain filtrate, concentrate the filtrate to 2/5 volume, obtain concentrate, add the anhydrous second of 2 times of concentrates
Alcohol, after standing 35min, centrifuging and taking lower sediment is simultaneously dried in vacuo, and obtains lemon dregs extract.
The preparation method of the gemma bacillus agent：Transferred from inclined-plane and cultivate bacillus, the seed liquor after spreading cultivation step by step
Transfer in fermentation tank, it is 30-35 DEG C to control temperature, aerlbic culture 48-60 hours, 90 revs/min of mixing speed, ventilation（V/
V）1:0.5, tank pressure 0.06MPa, fermentation finishes, and gemma bacteria agent is made in spray drying；
The preparation method of the gemma bacillus agent：Transferred from inclined-plane and cultivate bacillus, the seed liquor switching after spreading cultivation step by step
Enter in fermentation tank, it is 33 DEG C to control temperature, aerlbic culture 54 hours, 90 revs/min of mixing speed, ventilation（V/V）1:0.5, tank
0.06MPa is pressed, fermentation finishes, and gemma bacteria agent is made in spray drying；
The slant medium percentage by weight forms：Potato juice 20%, glucose 2%, agar 2.0%, remaining is pure
Water；
Spread cultivation step by step first order seed, secondary seed, three-level seed, first class seed pot, secondary seed tank and the fermentation life used
Production used medium percentage by weight, which forms, is：Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, remaining is pure water, pH 6.5.
Remaining is the same as implementation 2.
Test example 1：To preventing and treating grice diarrhoea rate effect test
Experimental animal selects twins（Group）Subordinate plant of limited company average weight is 7.5kg or so health
The miscellaneous piglet of " DLY " ternary 300, statistical analysis weight differences are not notable.Using single-factor Randomized Designs, by 300
Sodium selenite, by male and female half and half, it is divided into 6 groups, respectively 1 control group and 5 test groups, test group add embodiment 1- respectively
Feed addictive made from 5, control group do not add any additive made from embodiment 1-5.Every group of 5 repetitions, it is each to repeat
10 pigs.Continuous feeding 20 days, it is as follows to grice diarrhoea rate impact effect using invention feed addictive compared with control group：
| Project | Control group | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 |
| Diarrhea rate（%） | 18.4 | 3.2 | 2.8 | 3.4 | 16.4 | 17.4 |
In live pig feeding experiment, embodiment 1-3（Each technical parameter is in the range of the present invention program）Corresponding test group
Compared with control group, grice diarrhoea rate is remarkably decreased, also below embodiment 4 and embodiment 5.Test group pair corresponding to embodiment 2
Test pig diarrhoea prevention best results.
Test example 2：Anti-mold effect is tested
Test feed is twins（Group）The conventional feed of limited company's production, weighs 6 groups of feeds needed for experiment, often
Group 1000g, wherein 5 are separately added into obtained mold-proof oxidation-resistant agent in embodiment 1-5 as test group, pack after mixing, puts
In chamber, placed under conditions of 26~30 DEG C of temperature, relative humidity 75% or so, the 20th heaven-made first time visual inspection, this
Afterwards every the growing state of 5 days observation moulds；Remaining 1 group of feed does not add any mold-proof oxidation-resistant as blank control group
Agent, other same test groups.Experimental result is shown in Table 2.
Sampled after depositing to 55d, it is strict to press " in National Standard of the People's Republic of China's G B/T13092-2006 feeds
Total number of molds in the measure of total number of molds " measure each group feed.Each group feed needs to mix before sampling, and caking feed needs to crush, and fills
Divide after mixing and sample.Experimental result is shown in Table 3.
The feed of table 1 is in the feature in different storage stages
| Feed state | Criterion | Result of determination |
| It is not mouldy | Feed color is normal, can smell obvious feed fragrance | - |
| It is indistinctly mouldy | Feed color is normal, and feed fragrance is thin, indistinctly there is a musty | + |
| It is slight mouldy | Feed color shows slightly gloomy, can smell obvious musty | ++ |
| Moderate is mouldy | There is a small amount of point-like mildew, do not lump, musty is heavier | +++ |
| It is serious mouldy | Outward appearance has obvious point-like mildew, caking, and musty is very heavy | ++++ |
The observation result in each treatment group difference storage stage of table 2
3 each treatment group of table deposits the mould quantity to 55d
| Treatment group | Total plate count in feed（Individual/g） |
| Control group | 67×104 |
| Embodiment 1 | 11×104 |
| Embodiment 2 | 8×104 |
| Embodiment 3 | 12×104 |
| Embodiment 4 | 65×104 |
| Embodiment 5 | 68×104 |
It is can be seen that from above-mentioned experiment compared with blank control group, with the addition of the present embodiment 1-3 groups feed and conventional feed phase
Than the quantity of mould significantly reduces in the identical holding time, and feed storage life is longer, and embodiment 4-5 is poor compared with conventional feed
It is different little.
Claims (7)
1. a kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea, it is characterised in that by following raw material group by weight
Into：Oyster shell whiting 6-8 parts, tea extract 7-12 parts, lemon dregs extract 8-12 parts, Fructus Crataegi fermented product 10-14 parts, crab shell powder 5-
7 parts, montmorillonite 12-16 parts.
A kind of 2. mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea according to claim 1, it is characterised in that：By
Following raw material forms by weight：7 parts of oyster shell whiting, 10 parts of tea extract, 10 parts of lemon dregs extract, Fructus Crataegi fermented product 12
Part, 6 parts of crab shell powder, 14 parts of montmorillonite.
A kind of 3. mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea according to claim 1, it is characterised in that：
The preparation method of the green-tea extract：Take tealeaves to crushed 80-100 mesh sieves, obtain tea leaf powder, add tea leaf powder quality 5-
7 times of distilled water, stirs, and is heated to 35-40 DEG C, adds tea leaf powder quality 0.05-0.08% complex enzyme, stirs 30-
50min, absolute ethyl alcohol is added afterwards, is 40-50% to concentration of alcohol, extract 1-2h, filtering, obtain filtrate, be dried in vacuo,
Obtain tea extract；
The complex enzyme is by cellulase, pectase, protease in mass ratio 1:1-2:1-2 is formed；
The preparation method of the lemon dregs extract；Fresh Lemon slag is taken, IQF 30-40min under the conditions of being placed in -30 DEG C,
After crushing, it is placed in container and adds the water of 4-6 times of lemon dregs weight, heat 70-80 DEG C of keeping temperature, maintain 2-4h, afterwards will
Temperature is reduced to 37-42 DEG C, adds mixed enzyme, and mixed enzyme is pressed by 'beta '-mannase, pectase, zytase, cellulase
Weight is than 1:1-2:1.0-1.4:0.8-1.0 is formed, and the addition of mixed enzyme is the 0.1-0.14% of lemon dregs weight, and PH is adjusted
For 6-6.5, hydrolysis temperature is 37-42 DEG C, enzymolysis time 3-4h, is filtered after enzymolysis, obtains filtrate, concentrate the filtrate to 1/4-1/3
Volume, concentrate is obtained, add the absolute ethyl alcohol of 1.0-1.8 times of concentrate, after standing 20-30min, centrifuging and taking lower sediment is simultaneously
It is dried in vacuo, obtains lemon dregs extract；
The preparation method of the Fructus Crataegi fermented product：Hawthorn is taken, crushed 60-80 mesh sieves, hawthorn powder is obtained and is placed in container, adds and steams
Distilled water, the water content of hawthorn powder is reached 20-34%, add the gemma bacillus agent of mixture weight 0.05%, be sealed by fermentation 36-
48h, low-temperature air-drying is carried out after fermentation ends, obtains Fructus Crataegi fermented product；
The preparation method of the gemma bacillus agent：Transferred from inclined-plane and cultivate bacillus, the seed liquor switching after spreading cultivation step by step
Enter in fermentation tank, it is 30-35 DEG C to control temperature, aerlbic culture 48-60 hours, 90 revs/min of mixing speed, ventilation（V/V）1:
0.5, tank pressure 0.06MPa, fermentation finishes, and gemma bacteria agent is made in spray drying；
The slant medium percentage by weight forms：Potato juice 20%, glucose 2%, agar 2.0%, herba houttuyniae powder
0.8%, setose thistle powder 1.2%, remaining is pure water；
Spread cultivation step by step first order seed, secondary seed, three-level seed, first class seed pot, secondary seed tank and the fermentation life used
Production used medium percentage by weight, which forms, is：Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, lemon ground-slag 0.8%, tea leaf powder 1.2%, bark of eucommia powder 1.8%, remaining is pure water, pH 6.5.
A kind of 4. mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea according to claim 1, it is characterised in that：Institute
State the preparation method of green-tea extract：Take tealeaves to crushed 90 mesh sieves, obtain tea leaf powder, add the distilled water of 6 times of tea leaf powder quality,
Stir, be heated to 37 DEG C, add the complex enzyme of tea leaf powder quality 0.06%, stir 40min, add absolute ethyl alcohol afterwards, extremely
Concentration of alcohol is 45%, extracts 1.5h, filtering, obtains filtrate, be dried in vacuo, obtain tea extract；
The complex enzyme is by cellulase, pectase, protease in mass ratio 1:1.5:1.5 composition；
The preparation method of the lemon dregs extract；Fresh Lemon slag is taken, IQF 35min under the conditions of being placed in -30 DEG C, is crushed
Afterwards, it is placed in container and adds the water of 5 times of lemon dregs weight, heat 75 DEG C of keeping temperature, maintains 3h, temperature is reduced to 40 afterwards
DEG C, mixed enzyme is added, mixed enzyme is by 'beta '-mannase, pectase, zytase, cellulase by weight 1:1.5:1.2:
0.9 composition, the addition of mixed enzyme are adjusted to 6.3 for 0.12%, PH of lemon dregs weight, and hydrolysis temperature is 40 DEG C, enzymolysis time
3.5h, filter after enzymolysis, obtain filtrate, concentrate the filtrate to 1/3 volume, obtain concentrate, add the anhydrous second of 1.4 times of concentrates
Alcohol, after standing 205min, centrifuging and taking lower sediment is simultaneously dried in vacuo, and obtains lemon dregs extract；
The preparation method of the Fructus Crataegi fermented product：Hawthorn is taken, crushed 70 mesh sieves, hawthorn powder is obtained and is placed in container, adds distillation
Water, the water content of hawthorn powder is set to add the gemma bacillus agent of mixture weight 0.05% up to 27%, be sealed by fermentation 42h, fermentation knot
Low-temperature air-drying is carried out after beam, obtains Fructus Crataegi fermented product；
The preparation method of the gemma bacillus agent：Transferred from inclined-plane and cultivate bacillus, the seed liquor switching after spreading cultivation step by step
Enter in fermentation tank, it is 33 DEG C to control temperature, aerlbic culture 54 hours, 90 revs/min of mixing speed, ventilation（V/V）1:0.5, tank
0.06MPa is pressed, fermentation finishes, and gemma bacteria agent is made in spray drying；
The slant medium percentage by weight forms：Potato juice 20%, glucose 2%, agar 2.0%, herba houttuyniae powder
0.8%, setose thistle powder 1.2%, remaining is pure water；
Spread cultivation step by step first order seed, secondary seed, three-level seed, first class seed pot, secondary seed tank and the fermentation life used
Production used medium percentage by weight, which forms, is：Sucrose 6%, glucose 1%, De-fatted soya protein Powder 0.6%, potassium dihydrogen phosphate
0.1%, magnesium sulfate 0.2%, lemon ground-slag 0.8%, tea leaf powder 1.2%, bark of eucommia powder 1.8%, remaining is pure water, pH 6.5.
A kind of 5. mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea according to claim 1, it is characterised in that：Its
Preparation method is as follows：Oyster shell whiting, tea extract, lemon dregs extract, Fructus Crataegi fermented product, crab shell powder, montmorillonite are mixed and are
.
A kind of 6. mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea according to claim 1, it is characterised in that：
Addition in swine rationses is 800-1200g/ tons.
A kind of 7. mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea according to claim 1, it is characterised in that：
Addition in swine rationses is 1000g/ tons.
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|---|---|---|---|
| CN201710893522.2A CN107751607A (en) | 2017-09-28 | 2017-09-28 | A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea |
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|---|---|---|---|
| CN201710893522.2A CN107751607A (en) | 2017-09-28 | 2017-09-28 | A kind of mold-proof oxidation-resistant agent with preventing and treating pig epidemic diarrhea |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355545A (en) * | 2013-07-23 | 2013-10-23 | 南京林业大学 | Fermentation type botanical weaned piglet feed additive |
| CN105795143A (en) * | 2016-03-31 | 2016-07-27 | 双胞胎（集团）股份有限公司 | Compound feed additive for pigs |
| CN105831411A (en) * | 2016-04-14 | 2016-08-10 | 安徽省领航动物保健品有限责任公司 | Anti-diarrhea forage for lactation phase pig |
| CN106615808A (en) * | 2016-12-28 | 2017-05-10 | 梅州双胞胎饲料有限公司 | Pig feed additive capable of preventing and treating porcine respiratory syndromes and epidemic diarrhea |
-
2017
- 2017-09-28 CN CN201710893522.2A patent/CN107751607A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103355545A (en) * | 2013-07-23 | 2013-10-23 | 南京林业大学 | Fermentation type botanical weaned piglet feed additive |
| CN105795143A (en) * | 2016-03-31 | 2016-07-27 | 双胞胎（集团）股份有限公司 | Compound feed additive for pigs |
| CN105831411A (en) * | 2016-04-14 | 2016-08-10 | 安徽省领航动物保健品有限责任公司 | Anti-diarrhea forage for lactation phase pig |
| CN106615808A (en) * | 2016-12-28 | 2017-05-10 | 梅州双胞胎饲料有限公司 | Pig feed additive capable of preventing and treating porcine respiratory syndromes and epidemic diarrhea |
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