CN107737159A - A kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract - Google Patents
A kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract Download PDFInfo
- Publication number
- CN107737159A CN107737159A CN201711221605.3A CN201711221605A CN107737159A CN 107737159 A CN107737159 A CN 107737159A CN 201711221605 A CN201711221605 A CN 201711221605A CN 107737159 A CN107737159 A CN 107737159A
- Authority
- CN
- China
- Prior art keywords
- extract
- tea leaf
- eagle
- preparation
- leaf flavone
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract, methods described is to pre-process fresh Laoyin tea blade, extracted with water and obtain eagle tealeaves water extract, eagle tealeaves water extract is isolated and purified by flocculant precipitation, concentration, extraction, chromatographic step again, the final Laoyin tea leaf flavone extract for obtaining high content；Pharmacological evaluation shows that the Laoyin tea leaf flavone extract has certain effect of lowering blood sugar, and effect is suitable with Acarbose.The extractant used in the eagle tea flavonoid extract preparation method of the present invention is food additives, is had a safety feature；Crospovidone is Beer Clarifier, and it is good to isolate and purify security for food composition；The Laoyin tea leaf flavone content 85 90% that the inventive method obtains, the rate of recovery are 38 50%；There is the inventive method technique to be simple and convenient to operate, flavones content is high, equipment investment is few, product can be used for the medium advantage of ordinary food.
Description
Technical field
The invention belongs to field of pharmaceutical technology, and in particular to a kind of with hypoglycemic activity Laoyin tea leaf flavone extract
Preparation method.
Background technology
Laoyin tea contains the compositions such as flavones, glycoside, organic acid, cumarin and tannin, and wherein flavonoids is the master of Laoyin tea
Composition is wanted, mainly there is Kaempferol, Kaempferol-glucose villous themeda and Quercetin-glucoside.Result of study shows that Laoyin tea can reduce
The blood-sugar content of normal mouse, prompt it can be by stimulating the cells secrete insulin of pancreas islet 13 to produce blood sugar reducing function.Adrenaline
Make blood glucose rise by decomposing hepatic glycogen, and Laoyin tea reduces the blood glucose value of adrenaline-induced hyperglycosemia rats, illustrates that it may
There is to suppress hepatic glycogenolytic caused by adrenaline and promote hepatic glycogen synthesis, so as to reduce the source of blood glucose,
Increase the outlet of blood glucose, hence it is evident that reduce blood glucose rise caused by metabolic disorder.Alloxan and Streptozotocin are pancreas islet B thin
Born of the same parents kill agent, and optionally impaired isle B cell, causes artificial diabetes.In diabetic mice and rat model, always
Hawk tea has significant blood sugar reducing function, while the release to insulin in alloxan diabetes mice serum also there is promotion to make
With.Prompt it mainly by protecting pancreas islet p cells or promoting reparation and promotion insulin secretion, the increase blood of impaired mouth cells
Clear insulin content and reach the purpose for reducing blood glucose.
Diabetes are a kind of common multiple metabolic diseases, are often accompanied by the rise of blood fat, and glucose-lipid metabolism is disorderly and glycosuria
The occurrence and development of sick chronic complicating diseases are closely related.Therefore blood fat is reduced for preventing or delaying complication, improve patient of diabetes
The prognosis of person is significant.Capillary Electrophoresis (Capillary electrophoresis, CE) is a kind of efficient, quick
With the isolation technics of low consumption, it is especially suitable for separating some and does not retain on HPLC or be difficult to the composition analyzed, and sample is pre-
Handle infringement problem that is relatively simple, or even being not required to consider post, the organic acid compound available for measure plant.For
The common organic acid compound without ultraviolet absorption group, typically surveyed using indirect ultraviolet method (indirect UV, IUV)
Fixed, CE-IUV measure organic acid compounds are a kind of fast and convenient methods.Laoyin tea (Litsea coreana Level)
Also known as Litsea coreana, white tea is commonly called as, is distributed in the ground such as Sichuan, Guizhou, Yunnan, Anhui, is south China various nationalities long-term drink among the people
A kind of plant alternative tea, there is removing toxicity for detumescence, improving eyesight benefit to think, promote the production of body fluid to quench thirst, inducing diaphoresis heatstroke prevention and other effects.Herein with 4,5-miaow
Azoles dicarboxylic acids (4,5-imidazolecarboxylic acid, IDC) is back-ground electolyte, is established a kind of quick, sensitive
The CE-IUV methods of organic acid compound are analyzed, the method is also applied to 7 kinds of organic acid compounds and flavones in Laoyin tea
Analysis.
The present invention is back-ground electolyte using 4,5- imidazole-2-carboxylic acids (IDC), with 20mmol/L IDC-0.5mmol/L ten
Six alkyl trimethyl ammonium bromides (cetytrimethylammonium bromide, CTAB) electrophoresis solution (pH 12.1), separation
Voltage is -30kV, and column temperature is 20 DEG C, long capillary tube 95cm, seven kinds of organic acid compounds (grass in separation determination Laoyin tea
Acid, formic acid, citric acid, malic acid, Cys, Pidolidone and acetic acid) and flavones content, this method method is easy,
Quickly, accurately.
The content of the invention
The invention provides a kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract, it is characterised in that
Methods described is carried out as follows：
(1) it will be crushed after the cleaning of fresh eagle tealeaves, drying and cross 20 mesh sieves, eagle tea-leaf power is obtained, by Laoyin tea
Leaf powder is with water with mass ratio 1:8~10 mixing after, be stirred at room temperature immersion it is wide 5 hours, then heat at 85~100 DEG C
2~3h of extraction is heated to reflux, is filtered after being cooled to 30~50 DEG C, obtains filtrate, i.e. eagle tealeaves water extract；
(2) stirring adds flocculant in the eagle tealeaves water extract that step (I) obtains, and it is small to stand 4~6 at room temperature
When, it is then centrifuged for, discards precipitation, obtains supernatant a；The flocculant is sodium alginate, chitosan or carboxymethyl cellulose；
(3) ethanol water is added after the supernatant a that step (2) obtains is concentrated under reduced pressure makes ethanol volume final concentration of 70
~90%, after standing 12 hours, take supernatant b to be concentrated under reduced pressure into concentrate without ethanol flavor, obtain medicinal extract；
(4) water is added to be made into mixed liquor the medicinal extract that step (3) obtains, then by mixed liquor extractant in 50~80 DEG C of conditions
Lower stirring is extracted for 30~90 minutes, and 30~40 DEG C are cooled to after extraction completely, stratification, obtains upper layer of extraction liquid with
Layer raffinate, is concentrated under reduced pressure into 0.3~0.5 times of mixeding liquid volume by upper layer of extraction liquid, produces eagle tea flavonoid crude extract；
The extractant is by organic solvent A and organic solvent B using volume ratio as 1000:1 mixing composition, organic solvent A is ethyl acetate
Or ethyl acetate and isopropanol are with volume ratio 5~9:1 mixed solution；Organic solvent B is acetic acid；
(5) Capillary Electrophoresis-indirect ultraviolet method using IDC as back-ground electolyte determines Laoyin tea mesoxalic acid, formic acid, apple
Acid, citric acid, Cys, Pidolidone, the content of acetic acid and flavones.
As preferred means, step (2) flocculant is added in the form of the flocculant aqueous solution of mass concentration 1~5%,
Flocculant aqueous solution is 1~5 with eagle tealeaves water extract volume ratio:1000.
As preferred means, step (2) flocculant is added in the form of the flocculant aqueous solution of mass concentration 1%, wadding
The solidifying agent aqueous solution is 2 with eagle tealeaves water extract volume ratio:1000.
As preferred means, the supernatant a decompression volumes that step (2) obtains are concentrated into the eagle tealeaves of step (I) acquisition
L/20 times of water extract volume, adding the ethanol water of volumetric concentration 95% makes ethanol volume final concentration of 70~90%.
As preferred means, extractant described in step (4) is by organic solvent A and organic solvent B using volume ratio as 1000:
2 mixing compositions, organic solvent A are ethyl acetate or ethyl acetate and isopropanol with volume ratio 8:1 mixed liquor；Organic solvent B
For acetic acid.
As preferred means, the volumetric usage of extractant described in step (4) and the volume ratio of mixed liquor are 2~5:1.
As further preferred means, step (5) is carried out as follows：Step (4) is obtained into eagle tea flavonoid
Crude extract precision measures 2ml, is mixed with equivalent 2ml acetonitriles, and strength shakes, take after standing appropriate solution in 10000rpm rotating speeds from
Heart 10min, 0.45 μm of membrane filtration of supernatant, then appropriate dilution or direct injected measure.
Embodiment
The linear relationship of embodiment 1. is investigated
The mixed organic acid reference substance solution of serial various concentrations is taken, is determined by the following procedure：
Quartz capillary is cleaned with 1mol/L hydrochloric acid, water, 1mol/L sodium hydroxides and water successively before using daily, is finally used
Buffer solution balances 10min.Electrophoresis interval uses wash buffer 10min twice, and buffer solution after 4 times using changing.Running buffer is molten
Liquid：20mmol/L IDC -0.5mmol/L CTAB (pH 12.1), capillary column：95cm × 75 μm, coolant temperature：20 DEG C,
Sample introduction：0.5psi × 5s, separation voltage：- 30kV, Detection wavelength：214nm.
Be ordinate with peak area (A), reference substance concentration (C, μ g/mL) be that abscissa draws standard curve, obtain linear
Regression equation and the range of linearity, oxalic acid, formic acid, the range of linearity of Pidolidone and acetic acid are 20-1000 μ g/mL, citric acid and
The range of linearity of malic acid is 20-500 μ g/mL, and the range of linearity of Cys is 50-1000 μ g/mL.According to 3 times of noises
Detection limit, oxalic acid, formic acid, citric acid, malic acid, Pidolidone and acetic acid, the line of flavones are determined than (S/N=3) corresponding concentration
Sexual intercourse and detection limit the results are shown in Table 1.
The linear relationship of the organic acid of table 1. and flavones, detection limit
The precision test of embodiment 2.
200 μ g/ml mixed reference substance solutions are taken, according to the assay method of embodiment 1, METHOD FOR CONTINUOUS DETERMINATION 6 times, calculating 7 kinds has
The peak area relative standard deviation (RSD) of machine acid and flavones, as a result oxalic acid, formic acid, citric acid, malic acid, Cys, L-
The peak area RSD of glutamic acid, acetic acid and flavones is respectively 1.85%, 1.67%, 1.98%, 2.03%, 2.16%, 1.71%,
1.84%, 6.75%, show that this method precision is good.
The replica test of embodiment 3.
6 parts of 1.0g Laoyin teas sample is weighed respectively, it is accurately weighed, sample solution is prepared as follows, and 7 kinds of measure is organic
The peak area of acid：
Laoyin tea sample is put in vacuum drying chamber dries 4h in 60 DEG C, 40 mesh sieves is crossed after grinding, precision weighs fine powder about
1.0g, it is placed in band plug conical flask, adds 10ml water, ultrasonic 1h, stand, filtering, with 5ml water washing residues, merging filtrate, water-bath
It is placed in after concentration in 10ml measuring bottles, adds water to scale, shaken up, precision measures 2ml sample solutions, is mixed with equivalent 2ml acetonitriles, by force
Power shakes, and takes after standing appropriate solution to centrifuge 10min in 10000rpm rotating speeds, 0.45 μm of membrane filtration of supernatant, then suitably
Dilution or direct injected measure.
As a result the peak area RSD of oxalic acid, formic acid, citric acid, malic acid, Cys, Pidolidone, acetic acid and flavones
Respectively 2.03%, 2.29%, 2.95%, 2.66%, 2.34%, 2.38%, 2.16%, 6.97%, show that the method repeats
Property is good.
The stability test of embodiment 4.
Sample solution is prepared as described in Example 3, it is organic respectively at 0,3,6,12,24h sample introductions are postponed, recording 7 kinds
The peak area RSD of sour peak area, as a result oxalic acid, formic acid, citric acid, malic acid, Cys, glutamic acid, acetic acid and flavones
Respectively 2.26%, 2.15%, 2.54%, 2.67%, 2.81%, 2.32%, 2.46%, 6.88%, show this method in 24h
Interior stabilization.
It can be seen that from above example result and use IDC as back-ground electolyte, with CTAB electrophoresis solution split-voltages
Seven kinds of organic acid compounds (oxalic acid, formic acid, citric acid, malic acid, Cys, Pidolidones in the Laoyin tea of measure
And acetic acid) and flavones content, this method method is easy, quick, accurate, and the preparation method is simple to operate, is walked without sterilizing
Suddenly, it is relatively low without special installation, short preparation period, cost.
The description to embodiment the invention is not restricted to more than, those skilled in the art according to disclosure of the present invention,
The creative work improvement and modification that be carried out need not be passed through on the basis of the present invention, all should protection scope of the present invention it
It is interior.
Claims (8)
1. a kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract, it is characterised in that methods described is by as follows
Step is carried out：
(1) it will be crushed after the cleaning of fresh eagle tealeaves, drying and cross 20 mesh sieves, eagle tea-leaf power is obtained, by eagle tea leaf powder
End is with water with mass ratio 1:8~10 mixing after, be stirred at room temperature immersion it is wide 5 hours, then heat at 85~100 DEG C and heat
Backflow 2~3h of extraction, filters after being cooled to 30~50 DEG C, obtains filtrate, i.e. eagle tealeaves water extract；
(2) stirring adds flocculant in the eagle tealeaves water extract that step (I) obtains, and stands 4~6 hours at room temperature, so
After centrifuge, discard precipitation, obtain supernatant a；The flocculant is sodium alginate, chitosan or carboxymethyl cellulose；
(3) by step (2) obtain supernatant a be concentrated under reduced pressure after add ethanol water make ethanol volume final concentration of 70~
90%, after standing 12 hours, take supernatant b to be concentrated under reduced pressure into concentrate without ethanol flavor, obtain medicinal extract；
(4) add water to be made into mixed liquor the medicinal extract that step (3) obtains, then mixed liquor is stirred under the conditions of 50~80 DEG C with extractant
Mix 30~90 minutes and extracted, 30~40 DEG C are cooled to after extraction completely, stratification, obtains upper layer of extraction liquid and lower floor's extraction
Extraction raffinate, upper layer of extraction liquid is concentrated under reduced pressure into 0.3~0.5 times of mixeding liquid volume, produces eagle tea flavonoid crude extract；It is described
Extractant is by organic solvent A and organic solvent B using volume ratio as 1000:1 mixing composition, organic solvent A is ethyl acetate or second
Acetoacetic ester is with isopropanol with volume ratio 5~9:1 mixed solution；Organic solvent B is acetic acid；
(5) Capillary Electrophoresis-indirect ultraviolet method using IDC as back-ground electolyte determines Extractive mesoxalic acid, formic acid, apple
Tartaric acid, citric acid, Cys, Pidolidone, the content of acetic acid and flavones.
2. there is the preparation method of hypoglycemic activity Laoyin tea leaf flavone extract as claimed in claim 1, it is characterised in that
Step (2) flocculant is added in the form of the flocculant aqueous solution of mass concentration 1~5%.
3. there is the preparation method of hypoglycemic activity Laoyin tea leaf flavone extract as claimed in claim 2, it is characterised in that
Step (2) flocculant aqueous solution is 1~5 with eagle tealeaves water extract volume ratio:1000.
4. there is the preparation method of hypoglycemic activity Laoyin tea leaf flavone extract as claimed in claim 2, it is characterised in that
Step (2) flocculant is added in the form of the flocculant aqueous solution of mass concentration 1%, flocculant aqueous solution and eagle Aqua Folium Camelliae sinensis
Extract volume ratio is 2:1000.
5. there is the preparation method of hypoglycemic activity Laoyin tea leaf flavone extract as claimed in claim 1, it is characterised in that
The supernatant a decompression volumes that step (2) obtains are concentrated into l/20 times of the eagle tealeaves water extract volume of step (I) acquisition,
Adding the ethanol water of volumetric concentration 95% makes ethanol volume final concentration of 70~90%.
6. there is the preparation method of hypoglycemic activity Laoyin tea leaf flavone extract as claimed in claim 1, it is characterised in that
Extractant described in step (4) is by organic solvent A and organic solvent B using volume ratio as 1000:2 mixing compositions, organic solvent A are
Ethyl acetate or ethyl acetate and isopropanol are with volume ratio 8:1 mixed liquor；Organic solvent B is acetic acid.
7. there is the preparation method of hypoglycemic activity Laoyin tea leaf flavone extract as claimed in claim 7, it is characterised in that
The volumetric usage of extractant described in step (4) and the volume ratio of mixed liquor are 2~5:1.
8. there is the preparation method of hypoglycemic activity Laoyin tea leaf flavone extract as claimed in claim 1, it is characterised in that
Step is carried out as follows in (5)：Step (4) acquisition eagle tea flavonoid crude extract is taken into 2ml sample solutions, with equivalent
2ml acetonitriles mix, and strength shakes, and take appropriate solution to centrifuge 10min, 0.45 μm of filter of supernatant in 10000rpm rotating speeds after standing
Membrane filtration, then appropriate dilution or direct injected measure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711221605.3A CN107737159A (en) | 2017-11-23 | 2017-11-23 | A kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711221605.3A CN107737159A (en) | 2017-11-23 | 2017-11-23 | A kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107737159A true CN107737159A (en) | 2018-02-27 |
Family
ID=61239680
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711221605.3A Pending CN107737159A (en) | 2017-11-23 | 2017-11-23 | A kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107737159A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113813204A (en) * | 2021-09-07 | 2021-12-21 | 北京工商大学 | New anti-saccharification application of Litsea Coreana tea, anti-saccharification product and preparation method thereof |
-
2017
- 2017-11-23 CN CN201711221605.3A patent/CN107737159A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113813204A (en) * | 2021-09-07 | 2021-12-21 | 北京工商大学 | New anti-saccharification application of Litsea Coreana tea, anti-saccharification product and preparation method thereof |
| CN113813204B (en) * | 2021-09-07 | 2023-08-25 | 北京工商大学 | New use of hawk tea in anti-saccharification and anti-saccharification product and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP2842957B1 (en) | Method for extracting and separating ginkgolides | |
| CN101062077B (en) | Method for preparing stevia whole stevioside and stevia whole flavone at the same time | |
| CN110618211B (en) | Method for extracting scutellaria chemical components by using eutectic solvent | |
| EP2828276B1 (en) | Rutin-rich extract of uncaria elliptica and method of preparation | |
| CN104147054A (en) | Ginkgo biloba leaf extract as well as preparation method and application thereof | |
| CN103815103B (en) | A kind of method extracting low-caffeine high content tea polyphenol from fresh tea leaf in its | |
| CN110663788A (en) | Tea extract and industrial preparation method and application thereof | |
| CN101401829A (en) | Wild Jinchai liveness extract, preparation and uses thereof | |
| CN101195646B (en) | Production method of stilbene glycoside extract | |
| CN105285643B (en) | A kind of preparation method of ginsenoside drink | |
| CN107737159A (en) | A kind of preparation method with hypoglycemic activity Laoyin tea leaf flavone extract | |
| CN104672194A (en) | Method for extracting and purifying procyanidine from waste grape seeds | |
| CN105012372A (en) | Method for extracting total flavones of persimmon leaves by semi-bionic enzyme extraction and application of total flavones | |
| CN103142474B (en) | With the composition and method of making the same that high purity bilobalide B is active component | |
| CN109053756A (en) | Phenylpropanoid Glycosides class ester catechin and its preparation method and application | |
| JP2012005413A (en) | Method for preparing fermented tea extract containing polymer polyphenol | |
| CN109528846A (en) | A kind of method of Alkaloids in Plants extraction purification | |
| CN103923138B (en) | A kind of preparation method of Nicotifiorin and application thereof | |
| CN107823188A (en) | A kind of compound flavones spansule and preparation method thereof | |
| CN107669739A (en) | A kind of compound flavone oral liquid and preparation method thereof | |
| CN112641826A (en) | Perilla seed extract and preparation method and application thereof | |
| CN102144683B (en) | Method for clarifying green tea extract liquid | |
| CN102078411B (en) | Preparation method of spreading hedyotis herb injection | |
| CN103142472B (en) | A kind of ginkolide B composition and method of making the same | |
| CN102786472A (en) | Method for extraction separation of huperzine A in all-grass of snake foot clubmoss and its hairy root by supercritical extraction-crystallization technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180227 |
|
| WD01 | Invention patent application deemed withdrawn after publication |