CN107727847A - A kind of blood vessel endothelial factor detection kit and its application method - Google Patents
A kind of blood vessel endothelial factor detection kit and its application method Download PDFInfo
- Publication number
- CN107727847A CN107727847A CN201710917820.0A CN201710917820A CN107727847A CN 107727847 A CN107727847 A CN 107727847A CN 201710917820 A CN201710917820 A CN 201710917820A CN 107727847 A CN107727847 A CN 107727847A
- Authority
- CN
- China
- Prior art keywords
- reagent
- blood vessel
- vessel endothelial
- endothelial factor
- detection kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/475—Assays involving growth factors
- G01N2333/515—Angiogenesic factors; Angiogenin
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/71—Assays involving receptors, cell surface antigens or cell surface determinants for growth factors; for growth regulators
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Endocrinology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of blood vessel endothelial factor detection kit, including reagent R1：Tris buffer solutions, NaN3, trehalose, Qula be logical, PEG 2000, solvent is purified water；Reagent R2：Tris buffer solutions, NaCl, NaN3, BSA, glycerine, the coated blood vessel endothelial factor polyclonal antibody of latex, solvent is purified water；The raw material of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelial factor polyclonal antibody and polyethylene p-chloromethyl styrene copolymer.The invention also discloses a kind of application method of blood vessel endothelial factor detection kit.Advantages of the present invention is：(1) kit of the present invention has compared with high detection sensitivity, and simple to operate, quick, detection only needs 10 minutes；(2) antigen antibody complex that the present invention is formed, good stability, high specificity；(3) it is used for automatic clinical chemistry analyzer, can carries out and promote on a large scale.
Description
Technical field
The present invention relates to technical field of medical examination, more particularly to a kind of blood vessel endothelial factor detection kit and its use
Method.
Background technology
VEGF (English：Vascular endothelial growth factor, referred to as：VEGF),
Early stage is also referred to as vascular permeability factor (English：Vascular permeability factor, referred to as：VPF), it is blood vessel endothelium
The HBGF (heparin-binding growth factor) of cell-specific, can induction of vascular in vivo
Newborn (induce angiogenesis in vivo).The factor can be effectively promoted revascularization, to medical research by important
Effect.
At present, the main still radio immunoassay of method of blood vessel endothelial factor is detected, it is well known that radio-immunity
Analytic approach has certain radioactive pollution, and application instrument is costly.
Therefore, it is badly in need of a kind of blood vessel endothelial factor detection kit pollution-free, running cost is low at present.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of blood vessel pollution-free, running cost is low
Endothelial factor detection kit and its application method.
The present invention is achieved by the following technical solutions：A kind of blood vessel endothelial factor detection kit, including it is only each other
Vertical reagent R1 and reagent R2 biliquid components, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelium because
Sub- polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 9%-11%.
One of preferred embodiment as the present invention, including reagent R1 independent of each other and reagent R2 biliquid components, including
Composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelium because
Sub- polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 10%.
One of preferred embodiment as the present invention, including reagent R1 independent of each other and reagent R2 biliquid components, including
Composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelium because
Sub- polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 10.5%.
One of preferred embodiment as the present invention, the pH of the Tris buffer solutions of the reagent R1 is 7.5.
One of preferred embodiment as the present invention, the pH of the Tris buffer solutions of the reagent R2 is 7.5.
A kind of application method of blood vessel endothelial factor detection kit, comprises the following steps：
(1) testing sample is mixed with reagent R1,37 DEG C of incubation 5min；
(2) reacted absorbance A 1 is determined with automatic clinical chemistry analyzer；
(3) mixed again with reagent R2,37 DEG C of reaction 5min；
(4) reacted absorbance A 2 is determined with automatic clinical chemistry analyzer；
(5) concentration of sample Vascular Endothelium Derived Factors is calculated according to absorbance change value.
One of preferred embodiment as the present invention, the reagent R1 of the step (1) and the reagent R2 of step (3) volume ratio
For 3:1.
One of preferred embodiment as the present invention, testing sample and the reagent R1 and reagent R2 compositions of the step (1)
Volume ratio between reagent solution is 1:50.
One of preferred embodiment as the present invention, in the step (2) and step (4), extinction is determined at wavelength 600nm
Spend A1 and A2.
One of preferred embodiment as the present invention, in the step (5), absorbance change value is Δ A, i.e., after A2 subtracts A1
Value.
The present invention compared with prior art the advantages of be：
(1) kit of the present invention has higher detection sensitivity, simple to operate, quick, from detecting out that result only needs
10 minutes；
(2) antigen antibody complex that the present invention is formed, good stability, there is certain absorbance at a particular wavelength,
High specificity；
(3) present invention can be used on automatic clinical chemistry analyzer, is adapted to full-automatic testing, can carry out and push away on a large scale
Extensively.
Embodiment
Embodiments of the invention are elaborated below, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
A kind of blood vessel endothelial factor detection kit of the present embodiment, including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelium because
Sub- polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 9%.
Embodiment 2
A kind of blood vessel endothelial factor detection kit of the present embodiment, including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelium because
Sub- polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 11%.
Embodiment 3
A kind of blood vessel endothelial factor detection kit of the present embodiment, including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelium because
Sub- polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 10%.
Embodiment 4
A kind of blood vessel endothelial factor detection kit of the present embodiment, including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelium because
Sub- polyclonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is whole
Content in body reagent R2 is 10.5%.
Embodiment 5
A kind of preparation method of above-described embodiment Vascular Endothelium Derived Factors detection kit of the present embodiment, including following step
Suddenly：
(1) reagent R1 processed：
1. according to the constituent content of reagent R1 in above-described embodiment, Tris is dissolved in purified water, stirred, adjusts pH
To formula institute indicating value, R1 buffer solutions are configured to；
2. according to the constituent content of reagent R1 in above-described embodiment, by NaN3, trehalose, Qula be logical, PEG-2000 is dissolved in
In R1 buffer solutions, stir, treat that all raw materials fully dissolve, that is, reagent R1 is made；
(2) reagent preparation R2：
1. according to the constituent content of reagent R2 in above-described embodiment, Tris is dissolved in purified water, stirred, adjusts pH
To formula institute indicating value, R2 buffer solutions are configured to；
2. according to the constituent content of reagent R2 in above-described embodiment, by NaCl, NaN3, BSA, glycerine be dissolved in R2 buffer solutions
In, stir, produce R2 dispersion liquids；
3. prepare the coated blood vessel endothelial factor polyclonal antibody of latex：
A. taking the latex microsphere that particle diameter is 80nm and 120nm, the EDAC for adding 50g/L is molten in 100mM MES buffer solutions
Liquid is mixed after being incubated mixing 1h in 37 DEG C of environment, and supernatant is removed in centrifugation；The NHS solution for adding 50g/L recovers to original volume,
Mix to be incubated in 37 DEG C of environment and mix 1h, centrifugation removes supernatant, must mix microballoon emulsion；
B. polyethylene p-chloromethyl styrene copolymer is added in microballoon emulsion is mixed, is copolymerized 80nm and 120nm particle diameters
Latex；Centrifugation, surplus materials is scattered in MES buffer solutions, 3 times repeatedly, most after recovery in MES buffer solutions to original
Volume, blood vessel endothelial factor polyclonal antibody is added, 3.5h is reacted at 37 DEG C, centrifuged, sediment is scattered in original volume
PBS in, 3 times repeatedly, finally sediment is scattered in the NHS buffer solutions of original volume, adds BSA 30g/L；
C.4 DEG C seal 48h up for safekeeping, obtain the coated blood vessel endothelial factor polyclonal antibody of finally required latex；
4. dissolve the obtained coated blood vessel endothelial factor polyclonal antibody of latex with R2 dispersion liquids, ultrasonic disperse, most
Obtained reagent R2 eventually.
Embodiment 6
A kind of application method of above-described embodiment Vascular Endothelium Derived Factors detection kit of the present embodiment, including following step
Suddenly：
(1) 4uL testing samples are mixed with 150uL reagents R1,37 DEG C of incubation 5min；
(2) reacted absorbance A 1 is determined at wavelength 600nm with automatic clinical chemistry analyzer；
(3) mixed again with 50uL reagents R2,37 DEG C of reaction 5min；
(4) reacted absorbance A 2 is determined at wavelength 600nm with automatic clinical chemistry analyzer；
(5) according to absorbance change value Δ A=A2-A1, the concentration of sample Vascular Endothelium Derived Factors is calculated.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (10)
1. a kind of blood vessel endothelial factor detection kit, it is characterised in that including reagent R1 independent of each other and reagent R2 biliquids
Body component, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelial factor are more
Clonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is in overall examination
Content in agent R2 is 9%-11%.
2. blood vessel endothelial factor detection kit according to claim 1, it is characterised in that including reagent independent of each other
R1 and reagent R2 biliquid components, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelial factor are more
Clonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is in overall examination
Content in agent R2 is 10%.
3. blood vessel endothelial factor detection kit according to claim 1, it is characterised in that including reagent independent of each other
R1 and reagent R2 biliquid components, including composition and corresponding content be：
Reagent R1：
Reagent R2：
The material composition of the coated blood vessel endothelial factor polyclonal antibody of latex is：Latex microsphere, blood vessel endothelial factor are more
Clonal antibody and polyethylene p-chloromethyl styrene copolymer, wherein, polyethylene p-chloromethyl styrene copolymer is in overall examination
Content in agent R2 is 10.5%.
4. according to the blood vessel endothelial factor detection kit described in claim any one of 1-3, it is characterised in that the reagent R1
Tris buffer solutions pH be 7.5.
5. according to the blood vessel endothelial factor detection kit described in claim any one of 1-3, it is characterised in that the reagent R2
Tris buffer solutions pH be 7.5.
6. a kind of method of blood vessel endothelial factor detection kit using as described in claim any one of 1-3, its feature exist
In comprising the following steps：
(1) testing sample is mixed with reagent R1,37 DEG C of incubation 5min；
(2) reacted absorbance A 1 is determined with automatic clinical chemistry analyzer；
(3) mixed again with reagent R2,37 DEG C of reaction 5min；
(4) reacted absorbance A 2 is determined with automatic clinical chemistry analyzer；
(5) concentration of sample Vascular Endothelium Derived Factors is calculated according to absorbance change value.
7. the application method of blood vessel endothelial factor detection kit according to claim 6, it is characterised in that the step
(1) reagent R1 and the reagent R2 of step (3) volume ratio is 3:1.
8. the application method of blood vessel endothelial factor detection kit according to claim 6, it is characterised in that the step
(1) volume ratio between the reagent solution that testing sample and reagent R1 and reagent R2 is formed is 1:50.
9. the application method of blood vessel endothelial factor detection kit according to claim 6, it is characterised in that the step
(2) and in step (4), absorbance A 1 and A2 are determined at wavelength 600nm.
10. the application method of blood vessel endothelial factor detection kit according to claim 6, it is characterised in that the step
Suddenly in (5), absorbance change value is Δ A, i.e. A2 subtracts the value after A1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710917820.0A CN107727847A (en) | 2017-09-30 | 2017-09-30 | A kind of blood vessel endothelial factor detection kit and its application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710917820.0A CN107727847A (en) | 2017-09-30 | 2017-09-30 | A kind of blood vessel endothelial factor detection kit and its application method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107727847A true CN107727847A (en) | 2018-02-23 |
Family
ID=61208569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710917820.0A Withdrawn CN107727847A (en) | 2017-09-30 | 2017-09-30 | A kind of blood vessel endothelial factor detection kit and its application method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107727847A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101511892A (en) * | 2006-06-29 | 2009-08-19 | 英维特罗根戴内尔公司 | Particles containing multi- block polymers |
| US20120010234A1 (en) * | 2007-12-05 | 2012-01-12 | University Of Cincinnati | Use of vegf-d in the diagnosis of lymphangioleiomyomatosis (lam) disease |
| CN102690383A (en) * | 2011-03-22 | 2012-09-26 | 北京化工大学 | Preparation method of styrene-p-chloromethyl styrene copolymer microspheres |
| CN102875719A (en) * | 2012-10-22 | 2013-01-16 | 郑州大学 | Preparation method of 10-micron to 100-micron chloromethylated crosslinked polystyrene microspheres |
| CN103134926A (en) * | 2013-02-27 | 2013-06-05 | 上海交通大学 | Magnetic microsphere carrier and its making method |
| CN104530234A (en) * | 2014-12-30 | 2015-04-22 | 安源生物科技(上海)有限公司 | Human VEGF detection combination reagent, reagent box and use method thereof |
| CN104761643A (en) * | 2003-08-01 | 2015-07-08 | 健泰科生物技术公司 | Anti-VEGF antibodies |
| US20160025738A1 (en) * | 2013-03-04 | 2016-01-28 | Iq Products B.V. | Prognostic marker to determine the risk for early-onset preeclampsia |
| CN106932588A (en) * | 2015-12-30 | 2017-07-07 | 上海复星长征医学科学有限公司 | Detection α1Kit of-microglobulin and preparation method thereof |
| CN107167588A (en) * | 2017-03-28 | 2017-09-15 | 马杰 | Antibody and kit for detecting vascular endothelial growth factor in serum |
-
2017
- 2017-09-30 CN CN201710917820.0A patent/CN107727847A/en not_active Withdrawn
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104761643A (en) * | 2003-08-01 | 2015-07-08 | 健泰科生物技术公司 | Anti-VEGF antibodies |
| CN101511892A (en) * | 2006-06-29 | 2009-08-19 | 英维特罗根戴内尔公司 | Particles containing multi- block polymers |
| US20120010234A1 (en) * | 2007-12-05 | 2012-01-12 | University Of Cincinnati | Use of vegf-d in the diagnosis of lymphangioleiomyomatosis (lam) disease |
| CN102690383A (en) * | 2011-03-22 | 2012-09-26 | 北京化工大学 | Preparation method of styrene-p-chloromethyl styrene copolymer microspheres |
| CN102875719A (en) * | 2012-10-22 | 2013-01-16 | 郑州大学 | Preparation method of 10-micron to 100-micron chloromethylated crosslinked polystyrene microspheres |
| CN103134926A (en) * | 2013-02-27 | 2013-06-05 | 上海交通大学 | Magnetic microsphere carrier and its making method |
| US20160025738A1 (en) * | 2013-03-04 | 2016-01-28 | Iq Products B.V. | Prognostic marker to determine the risk for early-onset preeclampsia |
| CN104530234A (en) * | 2014-12-30 | 2015-04-22 | 安源生物科技(上海)有限公司 | Human VEGF detection combination reagent, reagent box and use method thereof |
| CN106932588A (en) * | 2015-12-30 | 2017-07-07 | 上海复星长征医学科学有限公司 | Detection α1Kit of-microglobulin and preparation method thereof |
| CN107167588A (en) * | 2017-03-28 | 2017-09-15 | 马杰 | Antibody and kit for detecting vascular endothelial growth factor in serum |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101196518B (en) | Hepatitis virus type C immune body chemiluminescence method diagnostic reagent kit and its producing method | |
| WO2017008179A1 (en) | Reagent for high throughput combined detection of hepatitis c virus antigen and antibody | |
| CN102944679A (en) | Kit for performing retinol binding protein detection by using latex turbidimetry | |
| CN103278651A (en) | Kit for chemiluminescence immunity quantitative detection of MYO (myohaemoglobinnano) nano magnetic particle and preparation method of kit | |
| CN103293323B (en) | A kind of interstitialcellstimulating hormone (ICSH) nano magnetic particulate chemistry electrochemiluminescent immunoassay immue quantitative detection reagent box and preparation method thereof | |
| CN111122874A (en) | Kit for determining LN based on latex enhanced immunoturbidimetry, and preparation and use methods thereof | |
| CN102662064A (en) | Immunonephelometry kit for detecting lipid carrier protein related to neutrophils gelatinase and preparation method thereof | |
| CN104820102A (en) | Kit of detecting the content of Lp-PLA2 and CRP on the basis of chemiluminescence, and method and application thereof | |
| CN104807997A (en) | Kit for detecting CA125 and SP17 contents based on chemiluminiscence method as well as method and application thereof | |
| CN107782890A (en) | A kind of preparation method of type Ⅳ collagen protein detection kit | |
| CN107741494A (en) | A kind of preparation method of CER detection kit | |
| CN106645756A (en) | Kit for detecting NMP22 (Nuclear Matrix Protein 22) and preparation method thereof | |
| CN109298178A (en) | Cardiac myosin binding protein C(cMyBP-C based on immunomagnetic beads) time-resolved fluoroimmunoassay kit | |
| CN108152518A (en) | A kind of kit for measuring Serum CG and its preparation application method | |
| CN107727847A (en) | A kind of blood vessel endothelial factor detection kit and its application method | |
| CN107703290A (en) | A kind of preparation method of blood vessel endothelial factor detection kit | |
| CN107677840A (en) | A kind of parathyroid hormone detection kit and its application method | |
| CN104215778B (en) | Crosslinked magnetic particle of a kind of C peptide monoclonal antibody and preparation method thereof and the detection kit that comprises it | |
| CN103267866A (en) | Chemiluminescence immune quantitative detection kit for follicle-stimulating hormone nanometre magnetic particles, and preparation method thereof | |
| CN107703307A (en) | A kind of type Ⅳ collagen protein detection kit and its application method | |
| CN104634960B (en) | The preparation method of a kind of immune magnetic microsphere and the tonyred detection kit containing immune magnetic microsphere and detection method | |
| EP2606352A1 (en) | Assay for analytes using multiple receptors | |
| CN103308677B (en) | Chemiluminescent immune quantitative detection kit of estradiol nanometer magnetic particles and preparation method thereof | |
| CN107741504A (en) | A kind of preparation method of parathyroid hormone detection kit | |
| CN108918888A (en) | It is a kind of detect Endostatin latex enhancing immune than turbid kit and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180223 |
|
| WW01 | Invention patent application withdrawn after publication |