CN107723377A - Millet gelatinization point gene molecule marker and its application - Google Patents
Millet gelatinization point gene molecule marker and its application Download PDFInfo
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- CN107723377A CN107723377A CN201711029264.XA CN201711029264A CN107723377A CN 107723377 A CN107723377 A CN 107723377A CN 201711029264 A CN201711029264 A CN 201711029264A CN 107723377 A CN107723377 A CN 107723377A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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Abstract
The invention discloses a kind of millet gelatinization point gene molecule marker and its application, the molecular labeling includes CAAS3020 marks and SI038 marks, the 125bp of CAAS3020 amplifications fragment and the mark that the 325bp of SI038 amplifications fragment is high gelatinization point, the 127bp of CAAS3020 amplifications fragment and the mark that the 329bp of SI038 amplifications fragment is low gelatinization point, can mark as assisted Selection and be screened for breeding progeny material.
Description
Technical field
The present invention relates to plant gene field, and in particular to a kind of millet gelatinization point gene molecule marker and its application.
Background technology
Because ALK gene homologous clone does not find the variant sites of gelatinization point, thus it is speculated that millet gelatinization point character may
It is the quantitative character by controlled by multiple genes.Using Rapid Visco-Analyzer (RVA) method, to 180 families of " S80X paddy black greatly " RIL colonies
Tie up to Shijiazhuang, the seed of the 2 environment harvests in Changzhi has carried out cooking and eating quality and surveyed fixed sum data collection work.2 points of colony
Data statistics result shows that each index is in continuous normal distribution two testing sites, and it is typical case to illustrate correlation of attributes character
Quantitative character.And except gelatinization point and the Test Point Data of time to peak two distribution quite in addition to, disintegration value, abatement value and
3 indexs of recycled LDPE show wider variation in Shanxi Changzhi than Hebei Shijiazhuang.
The content of the invention
To solve the above problems, the invention provides a kind of millet gelatinization point gene molecule marker and its application.
To achieve the above object, the technical scheme taken of the present invention is：
Millet gelatinization point gene molecule marker, including CAAS3020 marks and SI038 marks, CAAS3020 amplifications
125bp fragment and SI038 amplification 325bp fragment be high gelatinization point mark, CAAS3020 amplification 127bp piece
Section and the mark that the 329bp of SI038 amplifications fragment is low gelatinization point.
Above-mentioned millet gelatinization point gene molecule marker can be used for the detection of breeding progeny material gelatinization point, so as to carry out
The assisting sifting of progeny material.
The invention has the advantages that：
The fragment of the 125bp of the CAAS3020 mark amplifications of this specific implementation fragment and the 325bp of SI038 mark amplifications
For the mark of high gelatinization point, the 127bp of CAAS3020 mark amplifications fragment and the 329bp of SI038 mark amplifications fragment
For the mark of low gelatinization point, it can mark as assisted Selection and be screened for breeding progeny material.
Brief description of the drawings
Fig. 1 is polymorphism primer screening signal in parent in the embodiment of the present invention；In figure, arrow is polymorphism primer.
Fig. 2 is that SI038 marks and CAAS3020 mark the scanning schematic diagram in colony in the embodiment of the present invention.
Fig. 3 is the schematic diagram of genetic linkage mapses in the embodiment of the present invention.
Fig. 4 is the schematic diagram of QTL positioning results in the embodiment of the present invention.
Embodiment
In order that objects and advantages of the present invention are more clearly understood, the present invention is carried out with reference to embodiments further
Describe in detail.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to limit this hair
It is bright.
Millet gelatinization point gene molecule marker, wherein the QTL contribution rates between the 3rd chromosome CAAS3020-SI038 marks
The fragment and the 325bp of SI038 amplifications fragment that maximum is the 125bp of 17.02%, CAAS3020 amplifications are high gelatinization point
Mark, CAAS3020 amplification 127bp fragment and SI038 amplification 329bp fragment be low gelatinization point mark.
This specific implementation is using 700 SSR markers of each chromosome of uniform fold millet to " S80X paddy black greatly " RIL colonies
Parent detected (as shown in Figure 1), therefrom filtered out the mark of 140 uniform fold full-length genomes to 180 familys
(as shown in Figure 2) is scanned, one is constructed and includes 9 linkage groups, 90 genetic linkage mapses marked.Such as Fig. 4 institutes
Shown, genetic linkage mapses total length is 1160.5cM, and average distance is 13.03cM between mark.With reference to 2 quality determination numbers
According to, carried out QTL positioning, altogether excavate 3 environment under QTL site 26, wherein there is multiple characters aggregation in the 3rd, 4,8 chromosomes
Phenomenon.3 characters are navigated on the 3rd chromosome can stablize hereditary and higher contribution rate main effect position in both environments
Point, laid a good foundation (as shown in Figure 3) for further finely positioning and gene excavating.Gelatinization point on the 3rd, 4,6 chromosomes,
QTL contribution rates between wherein the 3rd chromosome CAAS3020-SI038 marks are maximum, are 17.02%.To CAAS3020 and SI038
Two mark the gelatinization point data of amplified fragments and colony in colony again to carry out statistical comparison, and two marks are in isolate something lost
Pass, as shown in table 1, the 125bp of CAAS3020 amplifications fragment and the 325bp of SI038 amplifications fragment are high gelatinization point
Mark, the 127bp of CAAS3020 amplifications fragment and the 329bp of SI038 amplifications fragment are the mark of low gelatinization point, can be with
Mark as assisted Selection and screened for breeding progeny material.
Gelatinization point corresponding to CAAS3220 and SI038 amplified fragments in table 1 " S80X paddy black greatly " part family
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. millet gelatinization point gene molecule marker, it is characterised in that marked including CAAS3020 marks and SI038,
The 125bp of CAAS3020 amplifications fragment and the mark that the 325bp of SI038 amplifications fragment is high gelatinization point, CAAS3020
The 329bp of the 127bp of amplification fragment and SI038 amplification fragment is the mark of low gelatinization point.
2. the application of millet gelatinization point gene molecule marker as claimed in claim 1, it is characterised in that after breeding
For the screening of material.
3. the application of millet gelatinization point gene molecule marker as claimed in claim 1, it is characterised in that after breeding
For the detection of material gelatinization point.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711029264.XA CN107723377A (en) | 2017-10-19 | 2017-10-19 | Millet gelatinization point gene molecule marker and its application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711029264.XA CN107723377A (en) | 2017-10-19 | 2017-10-19 | Millet gelatinization point gene molecule marker and its application |
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| Publication Number | Publication Date |
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| CN107723377A true CN107723377A (en) | 2018-02-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201711029264.XA Pending CN107723377A (en) | 2017-10-19 | 2017-10-19 | Millet gelatinization point gene molecule marker and its application |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6423886B1 (en) * | 1999-09-02 | 2002-07-23 | Pioneer Hi-Bred International, Inc. | Starch synthase polynucleotides and their use in the production of new starches |
| CN107090494A (en) * | 2017-03-24 | 2017-08-25 | 张家口市农业科学院 | The molecular labeling related to millet code grain number character and its detection primer and application |
-
2017
- 2017-10-19 CN CN201711029264.XA patent/CN107723377A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6423886B1 (en) * | 1999-09-02 | 2002-07-23 | Pioneer Hi-Bred International, Inc. | Starch synthase polynucleotides and their use in the production of new starches |
| CN107090494A (en) * | 2017-03-24 | 2017-08-25 | 张家口市农业科学院 | The molecular labeling related to millet code grain number character and its detection primer and application |
Non-Patent Citations (4)
| Title |
|---|
| I BERTIN ET AL.: "SSCP-SNP in pearl millet--a new marker system for comparative genetics", 《THEOR APPL GENET》 * |
| 杨坤 等: "谷子SSR遗传连锁图谱的构建及主要农艺性状的QTL分析", 《 全国植物分子育种研讨会摘要集》 * |
| 王丹丹: "谷子农艺和品质性状与Waxy基因exon4-exon9区间RT-PCR分析", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
| 陈建伟主编: "《中药生物技术》", 28 February 2017, 中国中医药出版社 * |
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