CN107711494A - A kind of breeding method of cucumber chromosomal Single Segment Substitution Lines in Rice - Google Patents
A kind of breeding method of cucumber chromosomal Single Segment Substitution Lines in Rice Download PDFInfo
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- CN107711494A CN107711494A CN201711189828.6A CN201711189828A CN107711494A CN 107711494 A CN107711494 A CN 107711494A CN 201711189828 A CN201711189828 A CN 201711189828A CN 107711494 A CN107711494 A CN 107711494A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of high mildew-resistance, short fruit handle, the breeding method of thick pulp cucumber chromosomal Single Segment Substitution Lines in Rice, using short raw sense powdery mildew self-mating system cucumber D8 as female parent, it is male parent with the climing mildew-resistance self-mating system JIN5 508 of length, using hybridization, backcrossing and Molecular Marker Assisted Selection Technology, it is returned through continuous 12 generation, 4 generation selfed breedings, successfully obtain the high powdery mildew resisting green cucumber chromosome Single Segment Substitution Lines in Rice D85109267 of dwarf form, the genetic background of the substitution line is almost identical with susceptible maternal D8, unique difference is in the chromosome single slice that the disease-resistant length of male parent JIN5 508 that replaced in its genetic background is only 0.7cM.The short high powdery mildew resisting green cucumber new lines D85109267 of life has that internode is short, and florescence concentrates, and fruit is short, and the advantages that pulp thickness, available for the preparation of the cenospecies for the purpose of precocity, fruit picking is more concentrated, and early yield height, increases income more than 20%.
Description
Technical field
The present invention relates to a kind of breeding method of cucumber chromosomal Single Segment Substitution Lines in Rice.
Background technology
In agricultural production, cenospecies relative to conventional variety it is resistant stronger, yield is higher the features such as, thus, often
Often with there is higher cultivation benefit.85% or so kind is cenospecies on cucumber production.It is used in China's cucumber production at present
For kind based on the close thorn cucumber of long fruit type, this veriety is mainly shown as that growth potential is strong, disease resistance is strong, fruit length is typically 35
Cm, mouthfeel is more excellent, and fruit is long（Maximum accounting exceedes the 30% of fruit total）, pulp is relatively thin, relatively low ratio of female is this
The main deficiency of class cucumber variety.The short general growth potential of fruit type cucumber is weaker in cultivating at present, and disease resistance is not strong especially anti-white
The ability of powder disease and downy mildew is poor；Fruit length is typically more excellent in 20 cms, mouthfeel.Improve the anti-of this type kind
Characteristic of disease, improvement fruit it is long, pulp is thick etc., and character is technically badly in need of breaking through with improveing.
Increasingly diversified feature is presented in cucumber consumption market at present, in short fruit type cucumber more suitable for Modern Family's structure
With the needs of personalized consumption, but due to for many years pulp is thick, fruit not having substantive breakthroughs on the character selection such as long,
Cause related varieties distribution speed slow, therefore, fail to produce the short fruit handle of collection, thick pulp, prematureness for a long time all the time, resist
The elite hybrid of characteristic of disease.
The content of the invention
The technical problem to be solved in the present invention is the defects of overcoming prior art, there is provided a kind of high mildew-resistance, short fruit,
The breeding method of thick pulp cucumber chromosomal Single Segment Substitution Lines in Rice, the high powdery mildew resisting green cucumber dyeing of dwarf form that this method is cultivated
Body Single Segment Substitution Lines in Rice D85109267 be the high mildew-resistance of production, short fruit the Parents of, thick pulp cucumber Hybrid,
Solve the deficiencies in the prior art.
In order to solve the above-mentioned technical problem, the invention provides following technical scheme：
The present invention provides a kind of breeding method of cucumber chromosomal Single Segment Substitution Lines in Rice, and it is yellow with short raw sense powdery mildew self-mating system
Melon D8 is female parent, using the climing mildew-resistance self-mating system JIN5-508 of length as male parent, using hybridization, backcrossing and molecular marker assisted selection
Technology, mildew-resistance assisted Selection is carried out using SCAR mark, through the backcrossing of continuous 12 generation, 4 generation selfed breedings, it is high to obtain dwarf form
Powdery mildew resisting green cucumber chromosome Single Segment Substitution Lines in Rice D85109267.The genetic background of the substitution line is almost complete with susceptible maternal D8
It is exactly the same, it is mainly characterized by that short life, internode are short, and florescence concentrates, high mildew-resistance, fruit is short, the advantages that pulp thickness, with D8 only
One difference is in the chromosome single slice that the disease-resistant male parent JIN5-508 length that replaced in its genetic background is only 0.7cM（It is located at
On No. 1 chromosome of cucumber, the SSR at fragment both ends is respectively SSR16474 and SSR16887）.
Further, the breeding method specifically includes following steps：
S1, using short fruit, high sense powdery mildew cucumber self-mating system D8 for female parent, with long fruit, high powdery mildew resisting green cucumber self-mating system
JIN5-508 is male parent, and hybridization obtains F1；
S2, it is returned by the use of Susceptible parent D8 as recurrent parent, was carried out continuously for 12 generations, per generation backcross population is selected and remain first short
It is raw, the climing plant of length is all eliminated；
S3, be with SCAR mark assisting sifting resistant strain then with the plant of SCAR mark be probably mildew-resistance plant, will
It is planted, and in growth period by field natural occurrence, is further differentiated the plant of mildew-resistance, after being returned for 12 generations, nanism, is resisted
Powdery mildew complete stability；
S4, again continuous 4 generations of selfing and with SSR marker differential staining body replacement segment characterizations, while to the fruit commodity of substitution line
Property and quality are selected, and finally obtain short life, short fruit handle, thick pulp, high powdery mildew resisting green cucumber chromosome Single Segment Substitution Lines in Rice
D85109267, its chromosome single slice are defined by SSR16474 and SSR16887.
Further, the specific primer sequence of described SCAR mark is as shown in SEQ ID NO.1：
Positive strand primer：5' AAGAGGGTATTTTCGTCATTT 3'；Negative strand primer：5' AGGAACAAATGGGCAAAG 3'.
Further, the specific primer sequence of described SSR16472 marks is as shown in SEQ ID NO.2：
Leading：Draw after CACCCACGTGCTGTAAAAAG： ACCAGTTAACACGTCAATATTTTCT.
Further, the specific primer sequence of described SSR16881 marks is as shown in SEQ ID NO.3：
Leading：Draw after CCCTCTCAACATTTTCCACAA：CGAGGAGACTTGATGGGATG..
The beneficial effect that is reached of the present invention is：
Short life, short fruit, thick pulp, high powdery mildew resisting green cucumber chromosome Single Segment Substitution Lines in Rice D85109267 be mainly characterized by：
Short life（Plant blooms in 12-14 sections binds）, internode it is short, florescence concentrate, high mildew-resistance, short fruit handle, fruit is stingless to be had
Knurl, pulp is thick, and fruit is without bitter taste, with D8 unique differences in the disease-resistant male parent JIN5-508 dyeing that replaced in its genetic background
Body single slice（On No. 1 chromosome of cucumber, the SSR at fragment both ends is respectively SSR16472 and SSR16881）.With
D85109267 is that the maternal cenospecies prepared has that growth potential is strong, disease resistance is strong, isometric in fruit, and commodity is good, in good taste etc.
Feature is the short fruit of production, the Parents of thick pulp, high mildew-resistance Hybrid.
The high mildew-resistance dyeing of cucumber that the present invention is obtained using hybridization, backcrossing and Molecular Marker Assisted Selection Technology seed selection
Body Single Segment Substitution Lines in Rice（Defined by two special SSR markers at single slice both ends）.The seed selection of the substitution line is Gao Kangbai from now on
Powder is sick, thick pulp new breeds of cucumbers seed selection is provided required and superior parent material by short fruit.
The high powdery mildew resisting green cucumber new lines D85109267 of short life that the method for the present invention is cultivated has internode short, blooms
Phase is concentrated, and fruit is short, and the advantages that pulp thickness, available for the preparation of the cenospecies for the purpose of precocity, fruit picking is more concentrated, in early days
Yield is high, increases income more than 20%.The high powdery mildew resisting green cucumber chromosome Single Segment Substitution Lines in Rice of dwarf form that the method for the present invention is cultivated
D85109267 be the high mildew-resistance of production, short fruit the Parents of, thick pulp cucumber Hybrid, solve prior art
Deficiency.
Embodiment
The preferred embodiments of the present invention are illustrated below, it will be appreciated that preferred embodiment described herein is only used for
The bright and explanation present invention, is not intended to limit the present invention.
Embodiment 1：
Parent seed selection flow：
It is maternal：D8, quoted from the U.S., cane is sturdy, and top is bound and stopped growing with a branch of female flower when internode grows to 12-14 sections,
Blade dirty-green, fruit is short, and pulp is thick, pericarp dirty-green；Height sense powdery mildew.
Male parent：JIN5-508 is sprawling species, and plant is tall and big, internode length, and fruit length, fruit is from domestic variety " Tianjin long
Spring No. 5 "（Tianjin Kerun Agricultural Science ＆ Technology Co., Ltd.）In continuous selfing 8 generations of separation, long fruit is selected in each filial generation
The fruit of type, the high anti-powdery mildew of cucumber for no longer separating and obtaining to the 8th generation character of selfing, long fruit, long fruit are type self-mating system.
The chain molecular marker screening of powder mildew resistance：
Hybridize with height sense powdery mildew strain D8 and strain JIN5-508 of overgrowing and be selfed to obtain F2, using separating group's analytic approach
（Bulked segregation analysis, abbreviation BSA）With ISSR molecular labelings, screening and powder mildew resistance close linkage
ISSR mark and carry out SCAR mark conversion.
Specific method is：It is maternal and high mildew-resistance strain JIN5-508 as paternal hybrid using height sense powdery mildew strain D8
Obtain F1 and be selfed to obtain F2（170 plants）, using segregating population bulked segregant analysis (Bulked Segregant Analysis,
BSA screening) is marked.F2 segregating populations are divided into two groups according to its phenotype (such as mildew-resistance, sense powdery mildew)（Each 10 plants）,
With the polymorphism between 100 parents of ISSR primer screenings two, polymorphic primer pair DNA ponds are showed between parent and enter performing PCR expansion
Increase, polymorphism is not only showed between parent and the primer of corresponding polymorphism is showed between two DNA ponds share 6, i.e. UBC861,
UBC862, UBC865, UBC867, UBC809, UBC815, in F2 colonies（300 plants）The upper further checking for carrying out 6 primers,
It is final to determine UBC809 and cucumber powdery mildew resistance gene linkage（Primer UBC809 sequence is：AGAGAGAGAGAGAGAGG）
And SCAR mark is successfully translated into, mark is named as S2F+S2R, and its sequence is as follows：Positive strand primer：5'
AAGAGGGTATTTTCGTCATTT 3'；Negative strand primer：5'AGGAACAAATGGGCAAAG 3', it is yellow available for assisted Selection
Melon mildew-resistance character.With this SCAR mark assisted Selection backcrossing system's totally 12 generation, the resistance to powdery mildew complete stability of system is returned.
D85109267 seed selection（Base helps SSR）：High mildew-resistance backcrossing system by the backcrossing of 12 generations is selfed,
Chromosome segment substitution line is screened with SSR marker.Utilize 324 pairs of SSR primer pair generations being evenly distributed on 7 chromosomes of cucumber
It is to be identified to change, and identification obtains 7 anti-cucumber powdery mildew replacement fragments, respectively positioned at Chr.1, Chr.2, Chr.3, Chr.4
And Chr.6, in 63 plants of phenotypes in the individual plant of mildew-resistance, to screen Single Segment Substitution Lines in Rice as 13 plants, to 13 plants of single slice generations
It is continuous individual plant selfing to change, while carries out SSR marker detection and commodity, quality etc. preferably, finally obtains and comes from male parent JIN5-
508 0.7cM chromosome Single Segment Substitution Lines in Rice, the single slice both ends are defined by SSR（SSR16474 and SSR16887）, it is fixed
Entitled D85109267, itself and D8 difference are only in that the piece in the JIN5-508 0.7cM of having been replaced in its genetic background
Section, without the difference of other characters in addition to powder mildew resistance in phenotype.
Embodiment 2：
1st, the generation of D85109267 preparing hybrids 1 is utilized
With short fruit type（Fruit grows 15 cms）The combination of female series preparing hybrid may insure that F1 generation keeps short fruit characteristic, together
When, ratio of female is higher than 85%, and yield potential is big.
2nd, the basic expressions of the D85109267 cenospecies prepared are utilized
It is basic as the cross combination that male parent is prepared using D85109267 using stingless or thorn-reduced, gynoecy cucumber self-mating system as female parent
（F1）Performance：It is female strong（Ratio of female is more than 85%）, growth potential is strong, blade is big, and blade is thick, and fruit grows 17 cms, fruit handle
Extremely short or nothing, fruit is stingless, few knurl, and pericarp is green, and commodity is good, mildew-resistance, downy mildew and droop, individual plant picking fruit 15
More than individual, 115 grams or so of mean fruit weight.Suitable for In Middle And Lower Reaches of Changjiang River spring greenhouse cultivation.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., it should be included in the present invention's
Within protection domain.
Claims (5)
1. a kind of breeding method of cucumber chromosomal Single Segment Substitution Lines in Rice, it is characterised in that it is with short raw sense powdery mildew selfing
It is that cucumber D8 is female parent, using the climing mildew-resistance self-mating system JIN5-508 of length as male parent, is aided in using hybridization, backcrossing and molecular labeling
Selection technique, mildew-resistance assisted Selection is carried out using SCAR mark, through the backcrossing of continuous 12 generation, 4 generation selfed breedings, obtain short life
The high powdery mildew resisting green cucumber chromosome Single Segment Substitution Lines in Rice D85109267 of type.
A kind of 2. breeding method of cucumber chromosomal Single Segment Substitution Lines in Rice according to claim 1, it is characterised in that the training
Educate method and specifically include following steps：
S1, using short fruit, high sense powdery mildew cucumber self-mating system D8 for female parent, with long fruit, high powdery mildew resisting green cucumber self-mating system
JIN5-508 is male parent, and hybridization obtains F1；
S2, it is returned by the use of Susceptible parent D8 as recurrent parent, was carried out continuously for 12 generations, per generation backcross population is selected and remain first short
It is raw, the climing plant of length is all eliminated；
S3, be with SCAR mark assisting sifting resistant strain then with the plant of SCAR mark be probably mildew-resistance plant, will
It is planted, and in growth period by field natural occurrence, is further differentiated the plant of mildew-resistance, after being returned for 12 generations, nanism, is resisted
Powdery mildew complete stability；
S4, again continuous 4 generations of selfing and with SSR marker differential staining body replacement segment characterizations, while to the fruit commodity of substitution line
Property and quality are selected, and finally obtain short life, short fruit handle, thick pulp, high powdery mildew resisting green cucumber chromosome Single Segment Substitution Lines in Rice
D85109267, its chromosome single slice are defined by SSR16474 and SSR16887.
3. the breeding method of a kind of cucumber chromosomal Single Segment Substitution Lines in Rice according to claim 2, it is characterised in that described
SCAR mark specific primer sequence as shown in SEQ ID NO.1：
Positive strand primer：5' AAGAGGGTATTTTCGTCATTT 3'；Negative strand primer：5' AGGAACAAATGGGCAAAG 3'.
A kind of 4. breeding method of cucumber chromosomal Single Segment Substitution Lines in Rice according to claim 2, it is characterised in that
The specific primer sequence of described SSR16472 marks is as shown in SEQ ID NO.2：
Leading：Draw after CACCCACGTGCTGTAAAAAG： ACCAGTTAACACGTCAATATTTTCT.
5. the breeding method of a kind of cucumber chromosomal Single Segment Substitution Lines in Rice according to claim 2, it is characterised in that described
SSR16881 mark specific primer sequence as shown in SEQ ID NO.3：
Leading：Draw after CCCTCTCAACATTTTCCACAA：CGAGGAGACTTGATGGGATG.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113229142A (en) * | 2021-06-15 | 2021-08-10 | 扬州大学 | Method for quickly and effectively creating strong parthenocarpic cucumber strain |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596616A (en) * | 2004-09-10 | 2005-03-23 | 南京农业大学 | Hybridizing method for obtaining allotranssituation system for anti downy mildew of cucumbus |
| CN104521743A (en) * | 2014-09-16 | 2015-04-22 | 扬州大学 | Cultivation method of high-powdery-mildew-resistance, short-fruit-stem, and thick-flesh cucumber chromosome single segment substitution line |
-
2017
- 2017-11-24 CN CN201711189828.6A patent/CN107711494A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1596616A (en) * | 2004-09-10 | 2005-03-23 | 南京农业大学 | Hybridizing method for obtaining allotranssituation system for anti downy mildew of cucumbus |
| CN104521743A (en) * | 2014-09-16 | 2015-04-22 | 扬州大学 | Cultivation method of high-powdery-mildew-resistance, short-fruit-stem, and thick-flesh cucumber chromosome single segment substitution line |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113229142A (en) * | 2021-06-15 | 2021-08-10 | 扬州大学 | Method for quickly and effectively creating strong parthenocarpic cucumber strain |
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