CN107693847A - A kind of keratin/hydroxyapatite composite membrane, preparation method and applications - Google Patents
A kind of keratin/hydroxyapatite composite membrane, preparation method and applications Download PDFInfo
- Publication number
- CN107693847A CN107693847A CN201710937769.XA CN201710937769A CN107693847A CN 107693847 A CN107693847 A CN 107693847A CN 201710937769 A CN201710937769 A CN 201710937769A CN 107693847 A CN107693847 A CN 107693847A
- Authority
- CN
- China
- Prior art keywords
- keratin
- hydroxyapatite
- composite membrane
- preparation
- hydroxyapatite composite
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/227—Other specific proteins or polypeptides not covered by A61L27/222, A61L27/225 or A61L27/24
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/32—Proteins, polypeptides; Degradation products or derivatives thereof, e.g. albumin, collagen, fibrin, gelatin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/64—Use of materials characterised by their function or physical properties specially adapted to be resorbable inside the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/12—Materials or treatment for tissue regeneration for dental implants or prostheses
Abstract
The invention discloses a kind of keratin/hydroxyapatite composite membrane, preparation method and its application in periosteum, tooth-planting, artificial skin, dressing or bone renovating material.The composite membrane hierarchal arrangement is into two phase structure：One layer is into bone photo, and its composition is hydroxyapatite, and another layer is that its composition is keratin into cartilage phase.The preparation method is：Using the natural keratin of biomass, inorganic substances hydroxyapatite as major ingredient, by both, uniformly mixed suspension is laid on separate paper or polyfluortetraethylene plate, its natural subsidence, split-phase solidification are treated, obtains flexible, elastic keratin/hydroxyapatite composite membrane.The flexibility and the fragility of hydroxyapatite of the ingenious keratin-binding of the present invention, a kind of homogeneous composite membrane with preferable mechanical performance is made, the crude protein content of the film is 60~80%, and technique is easy and effective, is adapted to commercial application.
Description
Technical field
The present invention relates to a kind of keratin/hydroxyapatite composite membrane, preparation method and applications, more particularly to a kind of warp
Natural subsidence, split-phase solidification, and obtain flexible and there is keratin/hydroxyapatite composite membrane of two phase structure, preparation method
And its application, belong to technical field of biological materials.
Background technology
Using native protein and the hydroxyapatite with bioactivity prepares imitative periosteum or bone tissue material all the time
As biomaterial and the study hotspot of tissue engineering material.Native protein has excellent biocompatibility and osteoinductive,
The mechanics fragility of the inorganic constituents hydroapatite particles of natural sclerotin can also be relaxed.
At present, prepare the related article of bone tissue material using native protein and hydroxyapatite both at home and abroad and patent is situated between
Continue, it is as a result as follows：Patent CN106039402A, CN104117097A, CN103738932A, CN103184570A,
CN106178126A、CN103505761A、CN103656756A、CN103028145A、CN102940906A、
CN102302804A, CN102552985A, CN102491299A etc. are prepared compound using fibroin albumen and nanometer hydroxyapatite
Timbering material；Patent CN104826172, CN104353118, CN104404480A, CN103992499A, CN103893827A,
CN103920191A, CN103705976A, CN103785059A etc. are by collagen and the phosphoric acid including hydroxyapatite
The progress of calcium class is compound, prepares related bone renovating material.
Also more document and patent utilization keratin and hydroxyapatite prepare bone tissue material, wherein mostly using cold
Freeze dry or electrostatic spinning technique.Such as " Synthesis and characterization of Wool keratin/
hydroxyapatite nanocomposite[J].Journal of Biomedical Materials Research Part
B-Applied Biomaterials.2012,100B (4)：896-902. " and patent CN102908664A is by comprising calcium ion
Ionic calcium soln, the phosphate solution comprising phosphate anion and keratin co-precipitation in aqueous, then pass through punching
After washing and filtering, freeze-dried acquisition apatite/keratin porous compound support frame, the material as obtained by regulating and controlling lyophilized speed control
The aperture of material and form.Patent CN103394131A uses Poly(D,L-lactide-co-glycolide, hydroxyapatite and wool angle egg
Bai Gongtong prepares double-deck guide tissue regeneration film, wherein, its double-decker is by Poly(D,L-lactide-co-glycolide/sheep respectively
The complex of feather keratin forms perforated membrane through electrostatic spinning technique, and Poly(D,L-lactide-co-glycolide/hydroxyapatite is answered
Zoarium forms compacted zone after solution-cast, and the film formed is tunica fibrosa.
The content of the invention
The technical problems to be solved by the invention are：There is provided one kind exist two phase structure, have some strength and pliability,
It is degradable, also meet composite membrane of biomedical material requirement and preparation method thereof.
In order to solve the above problems, the technical solution adopted in the present invention is：A kind of keratin/hydroxyapatite is compound
Film, it is characterised in that the composite membrane hierarchal arrangement is into two phase structure：One layer is that its composition is hydroxyapatite, separately into bone photo
One layer is that its composition is keratin into cartilage phase.
Preferably, the thickness of the composite membrane is 50~200 μm, and tensile strength is 5~25mPa.
Present invention also offers a kind of preparation method of above-mentioned keratin/hydroxyapatite composite membrane, it is characterised in that with
The natural keratin of biomass, the hydroxyapatite of inorganic substances are major ingredient, and by both, uniformly mixed suspension is laid in
On separate paper or polyfluortetraethylene plate, its natural subsidence, split-phase solidification are treated, obtains flexible, elastic keratin/hydroxyapatite
Composite membrane.
Preferably, above-mentioned preparation method includes step in detail below：
Step 1)：The preparation of keratin solution：Natural keratin fiber is passed through 0.5~2 hour in absolute ethyl alcohol
Through dissolving, centrifuging, dialysing, concentration process after extraction, obtain keratin solution or directly use raw liquid of ceratin, add deionization
Water is diluted with standby to it；
Step 2)：The preparation of hydroxyapatite slurries：Weigh hydroxyapatite powder to add in deionized water, stir
It is placed in afterwards in ultrasonic wave and 5-10 minutes is vibrated with 100-200W power ultrasonic, obtains the hydroxy-apatite that concentration is 10~50g/L
Stone slurries；
Step 3)：The preparation of keratin/hydroxyapatite suspensions：The keratin aqueous solution and step that step 1) is obtained
2) the hydroxyapatite slurries obtained are well mixed with 10~30: 1 volume ratio, plasticizer are added, with 100- in ultrasonic wave
200W power ultrasonic vibration 5-10 minutes are uniformly dispersed；
Step 4)：The preparation of keratin/hydroxyapatite composite membrane：Keratin/hydroxyapatite is mixed uniformly outstanding
Supernatant liquid is laid in separate paper or polytetrafluoroethylene (PTFE) template, drying and forming-film after being stood in climatic chamber, gained film tweezers
First soaked 0.5~2 hour in the ethanol that mass concentration is 75~100% after taking-up, then simultaneously wind is cleaned with phosphate buffer
It is dry, that is, obtain pure composite membrane.
It is highly preferred that natural keratin fiber in the step 1) using human hair, wool, goats hair, camel hair, the rabbit hair or
Yak hair, discarded hair can be selected.
It is highly preferred that the concentration of the keratin aqueous solution after being diluted in the step 1) is 30~60mg/mL.
It is highly preferred that the plasticizer in the step 3) uses glycerine, polyethylene glycol 200, polyethylene glycol 400 and poly- second
Any one in glycol 800, its addition is 10~30mg/mL.
It is highly preferred that the design parameter stood in the step 4) is：20~40 DEG C of temperature, relative humidity 40~60%,
Time of repose 12~48 hours.
Present invention also offers a kind of above-mentioned keratin/hydroxyapatite composite membrane in periosteum, tooth-planting, leatheret
Application in skin, dressing or bone renovating material.
Compared with prior art, the beneficial effects of the present invention are：
(1) natural keratin and hydroxyapatite that the present invention uses are respectively provided with excellent biocompatibility, and natural angle
Albumen meets the basic demand of biomaterial because its special gene order can promote adhesion of the osteocyte to material；
(2) present invention can give play to the mechanics strong point of keratin and hydroxyapatite from the film-forming process of solution-cast,
The flexibility and the fragility of hydroxyapatite of ingenious keratin-binding, mutual tonifying for the deficiency, it is made a kind of equal with preferable mechanical performance
Matter composite membrane；
(3) spreading depth of two phase structure in composite membrane is changed by regulating and controlling the addition of hydroxyapatite, to be expired
The clinical membrane material needed of foot, and the technique is easy and effective, is adapted to commercial application.
Brief description of the drawings
Fig. 1 is keratin/hydroxyapatite composite membrane tensile stress strain curve made from embodiment 1；
Fig. 2 is the field emission scanning electron microscope of keratin made from embodiment 2/hydroxyapatite composite membrane section two phase structure
Picture；
Fig. 3 is after keratin made from embodiment 3/hydroxyapatite composite membrane culture mouse preosteoblast 1,3,7 day
Fluorescence microscope picture.
Embodiment
To become apparent the present invention, hereby with preferred embodiment, and accompanying drawing is coordinated to be described in detail below.
Embodiment 1
A kind of preparation method of keratin/hydroxyapatite composite membrane：
Wool is placed in the device of extraction and condensation cycle, successively using acetone extract 2 hours, absolute ethyl alcohol extraction 2
After hour, rinsed 3 times with deionized water, drying, to remove the dirt of wool surfaces, grease and part sebum, so as to be washed
Net hair.
Weigh 5g scoured wools and shred, according to 1: 20 bath raio be added to containing 1.9% vulcanized sodium, 7M urea,
In the mixed solution of 0.8% lauryl sodium sulfate (SDS), after 12 hours are mixed slowly under conditions of 50 DEG C, with from
Scheming 1000r/min filters off undissolved wool.Then wool lysate is moved into molecular cut off 3500D bag filter and put
Dialysed in distilled water, change a water within every 2 hours, being poured into open ware after taking-up dialyzate after three days is placed in divulging information
In cupboard, under magnetic stirring apparatus 60 ° with moderate-speed mixer, concentrated after accelerating moisture evaporation.Add ultra-pure water after weighing solidification amount
Wool keratin solution is diluted to 50mg/mL, so as to which the wool keratin aqueous solution be made.
Hydroapatite particles are weighed using hydroxyapatite and wool keratin weight ratio as 0.1: 10 ratio, in addition
State to be placed under ultrasonic wave 100W power after the wool keratin aqueous solution and be uniformly dispersed, then mix keratin/hydroxyapatite
Close suspension to be laid in 2cm × 2cm separate paper mould, 24 are stood in climatic chamber (25 DEG C, relative humidity 50%)
Hour after take out, in medicinal alcohol soak 1h after rinsed with phosphate buffer, air-dry produce the wool angle with some strength
Albumen/hydroxyapatite composite membrane.
Strip is made in the composite membrane, is understood after being stretched using Instron Microtester5948, the film breaks
Resistance to spalling is 10.4 ± 1.2mPa, and breaking length is up to 210%, as shown in Figure 1.
Embodiment 2
A kind of preparation method of keratin/hydroxyapatite composite membrane：
The rabbit hair is placed in the device of extraction and condensation cycle, successively using acetone extract 2 hours, absolute ethyl alcohol extraction 2
After hour, rinsed 3 times with deionized water, drying, to remove the dirt on surface, grease and part sebum, so as to obtain scoured wool.
Weigh 10g scoured wools and shred, 2mol/L sodium hydroxide solution is added to according to 1: 20 bath raio, at 70 DEG C
Under the conditions of mix slowly 5 hours after, filter off undissolved rabbit hair waste residue with centrifuge 1000r/min.Then lysate is moved
Enter to be placed in distilled water in molecular cut off 3500D bag filter and dialysed, change a water within every 2 hours, taken out after three days saturating
Be poured into open ware after analysis liquid and be placed in fume hood, under magnetic stirring apparatus 60 ° with moderate-speed mixer, after accelerating moisture evaporation
Concentrated.Add ultra-pure water that rabbit hair keratin solution is diluted into 40mg/mL after weighing solidification amount, it is water-soluble so as to which keratin be made
Liquid.
Hydroapatite particles are weighed as 0.3: 10 ratio using hydroxyapatite and keratin weight ratio, add above-mentioned angle
It is placed in vibrate 2 minutes under ultrasonic wave 200W power after protein solution and is uniformly dispersed, then mixes keratin/hydroxyapatite
Close suspension to be laid in 2cm × 2cm separate paper mould, 24 are stood in climatic chamber (25 DEG C, relative humidity 60%)
Hour after take out, in medicinal alcohol soak 1h after rinsed with phosphate buffer, air-dry produce the keratin with some strength/
Hydroxyapatite composite membrane.
Film is cut off using the mode of liquid nitrogen brittle failure, and its section observed with SEM, according to
Cross Section Morphology understands that obvious two phase structure occurs in composite membrane, it will be apparent that white particle point is hydroapatite particles, is seen
Fig. 2.
Embodiment 3
A kind of preparation method of keratin/hydroxyapatite composite membrane：
It is added to after human hair is cleaned according to 1: 20 bath raio in 3mol/L sodium hydroxide solution, under conditions of 50 DEG C
After mixing slowly 12 hours, filter off undissolved residue and move into lysate in molecular cut off 3500D bag filter and put
Dialysed in distilled water, taken out after three days dialyzate under magnetic stirring apparatus 60 ° with moderate-speed mixer, after accelerating moisture evaporation
Concentrated.Add ultra-pure water that human hair keratin solution is diluted into 60mg/mL after weighing solidification amount, it is water-soluble so as to which keratin be made
Liquid.
Hydroapatite particles are weighed as 0.7: 10 ratio using hydroxyapatite and keratin weight ratio, add above-mentioned angle
It is placed under ultrasonic wave 100W power and is uniformly dispersed after protein solution, then by keratin/hydroxyapatite mixing suspension
It is laid in 2cm × 2cm separate paper mould, is taken after standing 24 hours in climatic chamber (25 DEG C, relative humidity 50%)
Go out, in medicinal alcohol soak 1h after rinsed with phosphate buffer, air-dry produce keratin/hydroxy-apatite with some strength
Stone composite membrane.
Above-mentioned composite membrane is made into sequin with card punch to be placed in orifice plate, sterilized through medicinal alcohol, use phosphate-buffered
Liquid carries out cell culture under conditions of 37 DEG C after cleaning in 5% CO2gas incubator, and mouse preosteoblast is planted
In on composite membrane, the growth of fluorescence microscope cell was used after 1,3,7 day, as a result confirms that composite membrane has good life
Thing compatibility, with the extension of cell culture time, superficial cell drawout and it is distributed more and more intensive after breeding, sees Fig. 3.
Claims (9)
1. a kind of keratin/hydroxyapatite composite membrane, it is characterised in that the composite membrane hierarchal arrangement is into two phase structure：One
Layer is into bone photo, and its composition is hydroxyapatite, and another layer is that its composition is keratin into cartilage phase.
2. keratin/hydroxyapatite composite membrane as claimed in claim 1, it is characterised in that the thickness of the composite membrane is
50~200 μm, tensile strength is 5~25mPa.
3. the preparation method of keratin/hydroxyapatite composite membrane described in a kind of claim 1 or 2, it is characterised in that with angle
Albumen, hydroxyapatite are major ingredient, and by both, uniformly mixed suspension is laid on separate paper or polyfluortetraethylene plate, is treated
Its natural subsidence, split-phase solidification, obtain flexible, elastic keratin/hydroxyapatite composite membrane.
4. the preparation method of keratin/hydroxyapatite composite membrane as claimed in claim 3, it is characterised in that including following
Specific steps：
Step 1)：The preparation of keratin solution：Natural keratin fiber is passed through into the extraction of 0.5~2 hour in absolute ethyl alcohol
By dissolving, centrifugation, dialysis, concentration process, obtain keratin solution or directly use raw liquid of ceratin, add deionized water pair
It is diluted with standby；
Step 2)：The preparation of hydroxyapatite slurries：Weigh hydroxyapatite powder to add in deionized water, stir rearmounted
5-10 minutes are vibrated with 100-200W power ultrasonic in ultrasonic wave, the hydroxyapatite that concentration is 10~50g/L is obtained and starches
Liquid；
Step 3)：The preparation of keratin/hydroxyapatite suspensions：The keratin aqueous solution and step 2) that step 1) is obtained
To hydroxyapatite slurries be well mixed with 10~30: 1 volume ratio, add plasticizer, with 100-200W in ultrasonic wave
Power ultrasonic vibration 5-10 minutes be uniformly dispersed；
Step 4)：The preparation of keratin/hydroxyapatite composite membrane：By the mixed uniformly suspension of keratin/hydroxyapatite
It is laid in separate paper or polytetrafluoroethylene (PTFE) template, drying and forming-film after being stood in climatic chamber, gained film is taken out with tweezers
First soaked 0.5~2 hour in the ethanol that mass concentration is 75~100% afterwards, then cleaned and air-dried with phosphate buffer, i.e.,
Obtain pure composite membrane.
5. the preparation method of keratin/hydroxyapatite composite membrane as claimed in claim 4, it is characterised in that the step
1) natural keratin fiber in uses human hair, wool, goats hair, camel hair, the rabbit hair or yak hair.
6. the preparation method of keratin/hydroxyapatite composite membrane as claimed in claim 4, it is characterised in that the step
1) concentration of the keratin aqueous solution after being diluted in is 30~60mg/mL.
7. the preparation method of keratin/hydroxyapatite composite membrane as claimed in claim 4, it is characterised in that the step
3) plasticizer in uses any one in glycerine, polyethylene glycol 200, polyethylene glycol 400 and polyethylene glycol-800, and it adds
Dosage is 10~30mg/mL.
8. the preparation method of keratin/hydroxyapatite composite membrane as claimed in claim 4, it is characterised in that the step
4) design parameter of standing is in：20~40 DEG C of temperature, relative humidity 40~60%, time of repose 12~48 hours.
9. a kind of keratin/hydroxyapatite composite membrane described in claim 1 or 2 periosteum, tooth-planting, artificial skin,
Application in dressing or bone renovating material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710937769.XA CN107693847A (en) | 2017-10-10 | 2017-10-10 | A kind of keratin/hydroxyapatite composite membrane, preparation method and applications |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710937769.XA CN107693847A (en) | 2017-10-10 | 2017-10-10 | A kind of keratin/hydroxyapatite composite membrane, preparation method and applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107693847A true CN107693847A (en) | 2018-02-16 |
Family
ID=61183435
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710937769.XA Pending CN107693847A (en) | 2017-10-10 | 2017-10-10 | A kind of keratin/hydroxyapatite composite membrane, preparation method and applications |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107693847A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110106634A (en) * | 2019-05-13 | 2019-08-09 | 天津工业大学 | A kind of keratin PEO compound bio nano fibrous membrane and preparation method thereof and bandage |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010084481A1 (en) * | 2009-01-23 | 2010-07-29 | Royal College Of Surgeons In Ireland | Layered scaffold suitable for osteochondral repair |
| WO2010107236A2 (en) * | 2009-03-17 | 2010-09-23 | 주식회사 바이오폴 | Porous scaffold for regenerating cartilage and bone, and method for manufacturing same |
| WO2012134059A2 (en) * | 2011-03-31 | 2012-10-04 | 인제대학교 산학협력단 | Complex support body for regenerating bones and cartilage, method for manufacturing complex support body, and composition for treating bones and cartilage containing same as active ingredient |
| CN104941005A (en) * | 2015-05-18 | 2015-09-30 | 天津工业大学 | Preparation method of hydroxyapatite-enhanced keratin composite aggregate |
-
2017
- 2017-10-10 CN CN201710937769.XA patent/CN107693847A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010084481A1 (en) * | 2009-01-23 | 2010-07-29 | Royal College Of Surgeons In Ireland | Layered scaffold suitable for osteochondral repair |
| WO2010107236A2 (en) * | 2009-03-17 | 2010-09-23 | 주식회사 바이오폴 | Porous scaffold for regenerating cartilage and bone, and method for manufacturing same |
| WO2012134059A2 (en) * | 2011-03-31 | 2012-10-04 | 인제대학교 산학협력단 | Complex support body for regenerating bones and cartilage, method for manufacturing complex support body, and composition for treating bones and cartilage containing same as active ingredient |
| CN104941005A (en) * | 2015-05-18 | 2015-09-30 | 天津工业大学 | Preparation method of hydroxyapatite-enhanced keratin composite aggregate |
Non-Patent Citations (1)
| Title |
|---|
| HUANG TU ET AL: "Structural studies and macro-performances of hydroxyapatite-reinforced keratin thin films for biological applications", 《JOURNAL OF MATERIALS SCIENCE》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110106634A (en) * | 2019-05-13 | 2019-08-09 | 天津工业大学 | A kind of keratin PEO compound bio nano fibrous membrane and preparation method thereof and bandage |
| CN110106634B (en) * | 2019-05-13 | 2021-08-10 | 天津工业大学 | Keratin PEO composite biological nanofiber membrane, preparation method thereof and adhesive bandage |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | Silk fibroin/chitosan scaffold with tunable properties and low inflammatory response assists the differentiation of bone marrow mesenchymal stem cells | |
| Brown et al. | Ultrarapid engineering of biomimetic materials and tissues: Fabrication of nano‐and microstructures by plastic compression | |
| Amini et al. | Optimally porous and biomechanically compatible scaffolds for large-area bone regeneration | |
| Yokoyama et al. | Biomimetic porous scaffolds with high elasticity made from mineralized collagen—an animal study | |
| Ball et al. | Biocompatible evaluation of barium titanate foamed ceramic structures for orthopedic applications | |
| US20020055143A1 (en) | Bone precursor compositions | |
| Nseir et al. | Biodegradable scaffold fabricated of electrospun albumin fibers: mechanical and biological characterization | |
| Dhasmana et al. | Silk fibroin protein modified acellular dermal matrix for tissue repairing and regeneration | |
| US9758558B2 (en) | Whey protein isolate hydrogels and their uses | |
| Kim et al. | Fabrication of duck’s feet collagen–silk hybrid biomaterial for tissue engineering | |
| Liu et al. | Preparation and characterization of collagen–hydroxyapatite composite used for bone tissue engineering scaffold | |
| CN106132447A (en) | Bio-medical instrument | |
| CN110639063B (en) | Mineralized collagen bionic bone repair material modified by hyaluronic acid oligosaccharide | |
| CN104667349A (en) | Growth factor-loading silk fibroin/collagen bracket material and preparation method thereof | |
| CN104707180B (en) | BMP loaded silk fibroin/collagen scaffold material and preparation method thereof | |
| CN105521525A (en) | Porous composite scaffold for bone tissue engineering and preparation method therefor | |
| CN113908341A (en) | Injectable bone induction repair material and preparation method thereof | |
| Li et al. | 3D bioprinting of heterogeneous tissue-engineered skin containing human dermal fibroblasts and keratinocytes | |
| CN114028620B (en) | Mineralized artificial periosteum and preparation method and application thereof | |
| Li et al. | Effect of the interconnecting window diameter of hydroxyapatite scaffolds on vascularization and osteoinduction | |
| CN107693847A (en) | A kind of keratin/hydroxyapatite composite membrane, preparation method and applications | |
| Revati et al. | In vitro biodegradation, cytotoxicity, and biocompatibility of polylactic acid/napier cellulose nanofiber scaffold composites | |
| Parivatphun et al. | Biphasic scaffolds of polyvinyl alcohol with silk fibroin for oral and maxillofacial surgery based on mimicking materials design: fabrication, characterization, properties | |
| Yunoki et al. | Effect of collagen fibril formation on bioresorbability of hydroxyapatite/collagen composites | |
| CN107737378A (en) | A kind of keratin hydroxyapatite composite membrane improved using silane coupler, preparation method and applications |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180216 |