CN107690482A

CN107690482A - The microorganism through modification for the optimization production of 2,4 dihydroxy butyric acid

Info

Publication number: CN107690482A
Application number: CN201580080660.8A
Authority: CN
Inventors: P·索卡耶; G·贝斯泰尔-卡尔; L·迪蒙-赛格诺维特
Original assignee: Metabolic Explorer SA
Current assignee: Metabolic Explorer SA
Priority date: 2015-04-07
Filing date: 2015-04-07
Publication date: 2018-02-13
Anticipated expiration: 2035-04-07
Also published as: US10415062B2; BR112017021255A2; WO2016162712A1; US20180100169A1; EP3280694B1; CN107690482B; EP3280694A1

Abstract

The present invention relates to the genetically modified microorganism for producing 2,4 dihydroxy butyric acid by metabolic conversion xylose via 1,2,4 butantriol intermediates.The invention further relates to the method for producing 2,4 dihydroxy butyric acid, and it is carried out by cultivating the genetically modified microorganism in the fermentation medium and 2,4 DHB being reclaimed from the culture medium.

Description

The microorganism through modification for the optimization production of 2,4- dihydroxy butyric acid

Introduction

The present invention relates to genetically modified microorganism, for by via BT intermediate metabolic conversion wood Sugar produces 2,4- dihydroxy butyric acid.The invention further relates to the method for production 2,4- dihydroxy butyric acid, it passes through in fermented and cultured The genetically modified microorganism is cultivated in base and 2,4-DHB is reclaimed from the culture medium and is carried out.

2,4- dihydroxy butyric acid (i.e. 2,4-DHB or DHB), also referred to as 2,4- dihydroxy fourth (alkane) acid or 2,4- dihydroxy fourth Acid esters/salt, it is a kind of compound with high economic interests, because it may act as being used for chemical synthesis 2- hydroxyls -4- (methyl sulphur Generation) and-butyrate/salt (HMTB) (a kind of methionine analogs, in Animal nutrition, widely used food especially in poultry Product additive) precursor.In fact, HMTB can be included in animal feed as the low price amino acid surrogates of methionine, To dramatically increase meat yield (US2009/318715).

Routinely, by using Alpha-hydroxy-gamma-butyrolacton (2HBL) in the presence of methyl mercaptan and alkalescence or acidic catalyst Chemical synthesis process production HMTB.Industrially in three steps from malic acid (DE19735575A1, AU2004200948A), or in two steps prepared in known manner from gamma-butyrolacton (WO2008/022953A1) 2HBL.The problem of this two lines causes is the generation of the salt for the difficulty and highly significant for separating 2HBL, and the salt is then It must be removed.In addition, HMTB petrochemistry synthesis is economically impractical because it depend on using harmful material and The material and reagent of condition and costliness.

Recently, Alpha-hydroxy-γ-fourth can be changed into by 2,4-DHB in an aqueous medium by adjusting appropriate pH by having shown Lactone (2HBL) (US2013/0204016).

Therefore, even if not being used for the native metabolic pathway of the biochemical production of 2,4- dihydroxy butyric acid, it is used in microorganism Metabolic pathway of synthesizing caused by DHB represents a kind of attractive alternative solution, because it alleviates many mentioned above ask Topic.In fact, it is developed recently the metabolic engineering method dependent on the recombination expression of certain enzyme in microorganism：WO2012/ 056318th, WO2013/160762, WO2014/009435 and EP14306564.7 are particularly described by via different metabolic way Footpath glucose fermentation in the genetically modified microorganism produces 2,4-DHB.By spatially similar same based on acting on The reasonable engineering of the structurally and mechanically knowledge of candidate's enzyme of race's substrate, or by screening native enzyme and by rationally designing into one Step improves to obtain the most of enzymes identified in these patent applications.More specifically, WO2012/056318 identified three kinds it is non- Naturally occurring enzyme (apple acid kinase, maloyl semialdehyde dehydrogenase and DHB dehydrogenases, be mutated), they can be in microorganism It is middle to be overexpressed so that Metabolic Intermediate (L)-malic acid is changed into 2,4-DHB；WO2013/160762 requires the heterologous table of a variety of enzymes Reach, some of them are mutated and improve enzymatic activity and/or substrate affinity (apple acyl CoA synzyme or succinyl CoA:(L)- Malic acid-CoA transferases and/or apple acyl-CoA lyases；Apple acyl CoA reductases；With DHB dehydrogenases) with by metabolism Mesosome malic acid or succinyl-CoA or glycollyl-CoA change into 2,4-DHB；And WO2014/009435 method is depended on and incited somebody to action Metabolic Intermediate (L)-homoserine changes into 2- oxos -4 hydroxybutyric acid (OHB) and by recombinantly expressing two kinds of mutant enzymes (homoserine transaminase and DHB reductases) and OHB is reduced in 2,4-DHB.

The present invention, which proposes, to be used in two one steps from 1,2,4- butantriol Metabolic Intermediates with micro-organisms The alternative route of synthesis of 2,4- dihydroxy butyric acid, the step need that BT continuously is oxidized into 2,4- dihydroxies Base butyraldehyde, 2,4- dihydroxy butyraldehydes are then oxidized to 2,4-DHB.

The present inventor really it was surprisingly found that can by that carbon source (more particularly xylose) can changed into 1,2, Total output that is genetically engineered and substantially improving 2,4-DHB is carried out to above-mentioned two reaction in the microorganism of 4- butantriols.

Therefore, the present invention provides alternative microorganism herein, and its is genetically modified for optimizing production from xylose 2,4- dihydroxy butyric acid.This microorganism of xylose into 1,2,4- butantriols is included further through genetically engineered Genetic modification with：

- BT is oxidized to 2,4- dihydroxy butyraldehydes, and

- 2,4- dihydroxy butyraldehydes are oxidized to 2,4-DHB.

The invention further relates to by fermenting and producing 2, the method for 4- dihydroxy butyric acid, it is included in the culture medium containing xylose The microorganism of the present invention is cultivated, and from culture medium 2,4-DHB caused by recovery.

Detailed description of the invention

It should be understood that following detailed description is not restricted, and can be without departing substantially from the scope of the present invention In the case of carry out it is various modification, replace, omit and change.It should also be understood that terms used herein is to describe the present invention Specific embodiment purpose, and be not intended to be restricted.

Herein cited all publications, patents and patent applications (either above still hereafter) are stated with it by carrying Entirety is hereby incorporated into.

In addition, unless otherwise indicated, all technologies used herein and scientific terminology have and ordinary skill people The identical implication that member is generally understood that.Conventional microbiology and Protocols in Molecular Biology is also known in the art and conventional Those.Such technology is well known to the skilled artisan, and fully explain in the literature (see, for example, Sambrook etc., 2012).

Nevertheless, for the use through the different terms of this specification, following definitions are particularly applicable.

Unless the context, singulative " one kind ", "one" and " should/described " include multiple herein Number is censured.Thus, for example, referring to that " microorganism " includes this variety of quasi-microorganism, and refer to that " endogenous gene " is to refer to one Or multiple endogenous genes, etc..

Term "comprising", " containing ", " being related to " or " comprising " or modification herein with comprising meaning use, i.e., with The presence of the full and clear feature, but it is not excluded for the presence or addition of further feature in each embodiment of the present invention.

As used herein, term " microorganism " refers to microscopic organism living, and it can be unicellular or multicellular organism, and lead to It can often be found in nature.In the context of the present invention, microorganism is preferably bacterium, yeast or fungi.It is highly preferred that this The microorganism of invention is selected from enterobacteriaceae (Enterobacteriaceae), Bacillaceae (Bacillaceae), Clostridiaceae (Clostridiaceae), Streptomycetaceae (Streptomycetaceae), bar Cordycepps (Corynebacteriaceae) and ferment It is female.Even further preferably, the microorganism of the present invention is Escherichia (Escherichia), Klebsiella (Klebsiella), hot anaerobic bacillus(cillus anaerobicus) category (Thermoanaerobacterium), fusobacterium (Clostridium), Corynebacterium (Corynebacterium) or saccharomyces strain.Even more preferably still, microorganism of the invention is selected from Escherichia coli (Escherichia coli), Friedlander's bacillus (Klebsiella pneumoniae), hot Thermoanaerobactersaccharolyticum (Thermoanaerobacterium thermosaccharolyticum), clostridium acetobutylicum (Clostridium Acetobutylicum), Corynebacterium glutamicum (Corynebacterium glutamicum) and saccharomyces cerevisiae (Saccharomyces cerevisiae).Most preferably, microorganism of the invention is Escherichia coli.

As used herein, term " recombinant microorganism ", " genetically modified microorganism " or " genetically engineered microorganism " Refer to microorganism as defined above, the microorganism can not find in nature, therefore genetically with its natural homologue It is different.In other words, it refers to by importing and/or by missing and/or by modifying its genetic elements and micro- life through modification Thing.Such modification can force the shape of novel metabolic pathways by genetic engineering, by cultivating microorganism under specific selection pressure Carried out into evolution or by combining both approaches (see, for example, WO2005/073364 or WO2008/116852).

Therefore, mean that the microorganism is energy according to the genetically modified microorganism for being used to produce 2,4-DHB of the present invention Enough produce 2,4-DHB recombinant microorganism as defined above.In other words, the microorganism is genetically modified and allow to produce Raw 2,4-DHB.

In the context of the present invention, can be micro- come genetic modification by adjusting the expression of one or more endogenous genes Biology.The expression that " regulation " refers to the gene herein raised compared with its natural expression, is lowered or even It is completely eliminated.Therefore, such regulation can cause the enhancing of gene product activity, or alternatively cause endogenous gene products Compared with low activity or inactive.

Term " endogenous gene " in this article refers to be naturally occurring in the gene in microorganism.

The heterologous sequence of up-regulation can be advantageous in addition to endogenous regulation element by introducing or be replaced by using such heterologous sequence Change those endogenous regulation elements, or the chromosome by the way that one or more supplement copies of endogenous gene are introduced in microorganism Or plasmid is overexpressed endogenous gene.Also can be endogenous to modify with modifier product by the way that mutation is introduced into its coded sequence Gene activity and/or expression.The missing of endogenous gene can also be carried out to completely inhibit its expression in microorganism.Regulation Endogenous gene expression another way be with stronger or weaker promoter exchange its promoter (i.e. wild-type promoters) with Raise or lower the expression of this gene.Promoter suitable for such purpose can be homologous or heterologous and be this area In it is known.Appropriate promoter is selected to adjust the expression of endogenous gene in the limit of power of those skilled in the art.

Additionally or alternatively, microorganism can be genetically modified and expresses one or more foreign genes, and condition is by the gene Imported necessary to being expressed for it in host microorganism together with all regulating elements in microorganism.With exogenous DNA modification or " conversion " microorganism is the normal work to do of those skilled in the art.In the context of the present invention, term " overexpression " is herein In also used for expression of the foreign gene in microorganism.

It is not naturally occurring in microorganism that " foreign gene " or " heterologous gene " refers to the gene herein.In order to The expression alien gene in microorganism, this genoid can be integrated directly into microbial chromosomal, or by microorganism Plasmid or carrier dyeing vivoexpression.A variety of plasmids (different in terms of its replication orgin and its copy number in cell) It is well known in the present art, and can be readily selected for this purpose by those of skill in the art.According to the external source base of the present invention Because being advantageously homologous gene.

In the context of the present invention, term " homologous gene " or " homologue " are referred not only to by heredity common in theory Ancestors by the heredity of two species (i.e. microbial species) gene, and including can for it is genetically unrelated but evolved with encode into Row identity function and/or with similar structure protein gene (i.e. function homologue).Therefore, term " functional homologue " Refer to the gene of encoding function homologous protein herein.

Using database, as Uniprot (being used for protein), Genbank (being used for gene) or NCBI (be used for protein or Gene) in available information, those skilled in the art can be readily determined the specified protein of microorganism and/or the sequence of gene Row, and based on one of the equivalent gene in this Sequence Identification another kind microorganism or homologue.This routine work can pass through one kind (it can be looked for the gene order or genome of the specific gene sequence of microorganism and other microorganisms in database mentioned above To) sequence alignment carry out.Advantageously, the BLAST algorithm of the exploitation such as Altschul (1990) can be used to carry out such sequence Compare.Once establish sequence homology between those sequences, can draw consensus sequence and for design degeneracy probe with gram The corresponding homolog genes of grand related microorganisms.These conventional methods of molecular biology be well known to a person skilled in the art.

It is to be further understood that in the context of the present invention, if expression encodes sense in specific microorganism The foreign gene of the protein of interest, then preferably by the way that not preferred codon or less preferred codon are replaced with into coding The preferred codon of the microorganism of same amino acid builds the synthesized form of this gene.The really known codon in this area Selection changes between microbial species, and it is horizontal that this can influence recombinant expression of proteins interested.In order to overcome this problem, Codon optimization method has been developed, and has been retouched extensively by (2001) such as Graf etc. (2000), Deml and Davis＆Olsen (2011) State.It is interesting to note that in order to which codon optimization determines and develops several softwares, such asSoftware (Lifetechnologies) or (GenScript) OptimumGene^TMSoftware.In other words, the outer of proteins of interest matter is encoded Source gene preferably carries out codon optimization and is used to express in specified microorganisms.

Also can activity that is genetically modified and increasing or decreasing one or more protein according to the microorganism of the present invention.

Can by improve protein catalytic efficiency, by reduce Protein Turnover rate, by reduce mRNA (mRNA) week Rate of rotation, the transcription by increasing gene or the translation by increasing mRNA, which obtain, increases such activity.

Improve protein catalytic efficiency to mean, for given substrate and/or given co-factor, to increase kcat and/or reduction Km, and/or for giving inhibitor, increase Ki.Kcat, Km and Ki are the Michaelis- that those skilled in the art can determine Menten constants (Segel, 1993).Reduce Protein Turnover rate and mean stable protein.Improve protein catalytic efficiency and/or Reduce Protein Turnover rate method be well known to a person skilled in the art.Those include rational engineered and sequence And/or structural analysis and directed mutagenesis, and random mutagenesis and screening.Conventional method such as PCR can be passed through (PCR) direct mutagenesis is carried out, by random mutagenesis techniques for example via mutagens (ultraviolet or chemical reagent such as nitrosoguanidine (NTG) or ethyl methane sulfonate (EMS)) DNA reorganization or fallibility PCR introduce mutation.Stable protein also can be by protein N- ends or C- ends addition " label " peptide sequence realize.Such label is well known in the present art, and including paddy Sweet peptide-S- transferases (GST) of Guang etc..

MRNA can be reduced by modifying 5 '-non-translational region (5 '-UTR) and/or code area and/or 3 '-UTR gene order Turnover rate (Carrier and Keasling, 1999).

The transcription (either endogenous or external source) for increasing gene can be by increasing the number that it is copied in microorganism And/or realized by using the promoter of higher gene expression dose is caused compared with wild-type promoters.In the present invention Context in, term " overexpression " be also used for refer to microorganism in genetic transcription increase.

It is as indicated above, can be on chromosome or in chromosome outer coding in order to increase the copy number of gene in microorganism The gene.When to encode gene interested on chromosome, can by genetic recombinant methods well known in the art (such as Gene substitution) on chromosome introduce gene several copies.When will in microorganism in chromosome outer coding gene when, it can Carried by different types of plasmid, the plasmid depended on for its replication orgin their microorganisms that can replicate wherein with And their copy numbers in cell and it is different.According to the property of plasmid, the microorganism converted by the plasmid can contain plasmid 1 to 5 copy, or its about 20 copy, or even its up to 500 copy.The low-copy that can be replicated in Escherichia coli The example of number plasmid include but is not limited to pSC101 plasmids (close replicate), RK2 plasmids (close to replicate) and pACYC and PRSF1010 plasmids, and the example for the high copy number plasmid that can be replicated in Escherichia coli is pSK bluescript II.

The promoter that gene expression dose can be increased is also that well known to a person skilled in the art and can be homologous (rise Come from identical species) or heterologous (originating from different species).The example of such promoter includes but is not limited to promoter Ptrc, Ptac, Plac and λ promoter cI.These promoters also can be by specific compound or by specific external condition such as temperature Degree or photoinduction (" inducible promoter ").

MRNA translation can be increased by modifying ribosome bind site (RBS).RBS is when starting protein translation and core The sequence on mRNA that sugared body combines.It can be initiation codon AUG upstreams in mRNA 5 ' caps, prokaryotes in eucaryote Internal ribosome entry site (IRES) in 6-7 nucleotides area (being referred to as Shine-Dalgarno sequences) or virus.Pass through This sequence is modified, protein translation initial rate can be changed, changes its throughput rate in proportion, and controls its work in the cell Property.Also the intensity of RBS sequences can be optimized to reach targeting by using software RBS CALCULATOR (Salis, 2011) Translation initiation speed.Property based on mRNA selects RBS sequences in the limit of power of those skilled in the art.

The activity for reducing protein can refer to reduce its special catalysis by being mutated the gene of code for said proteins Activity, so as to change corresponding amino acid sequence by mutant nucleotide sequence or by lacking the code area of the gene And/or reduce the concentration of protein in cell.

As used herein, term " oxidoreducing enzyme " refers to according to operating condition reversibly or irreversibly reduction or oxygen Change the enzyme of its substrate.More specifically, this fermentoid is transferred to referred to as oxidant by being catalyzed electronics from its substrate (electron donor) Another molecule of (electron acceptor) and work.In the context of the present invention, they are each for the redox oxydasis of selection From substrate (BT is oxidized to 2,4- dihydroxy butyraldehydes, and 2,4- dihydroxy butyraldehydes are oxidized to 2,4-DHB), more preferably Carry out in an irreversible fashion.Operating condition is adapted to so that their substrate of the redox oxydasis of the present invention is in this area skill In the limit of power of art personnel.

Oxidoreducing enzyme is generally categorized as the EC1 in the enzyme committee (EC) number class of enzyme, it is grouped more than 20 kinds Subclass (such as EC1.1, including act on the oxidoreducing enzyme of the CH-OH groups of donor, also referred to as alcohol oxidoreducing enzyme of enzyme；EC 1.2, including act on the aldehyde radical of donor or the oxidoreducing enzyme of oxo group；EC1.3, including act on the CH-CH bases of donor The oxidoreducing enzyme of group, also referred to as CH-CH oxidoreducing enzyme；EC1.4, including act on donor CH-NH2 groups oxidation also Protoenzyme donor, also referred to as amino acid oxidoreductases or monoamine oxidase；EC1.5, including act on the CH-NH groups of donor Oxidoreducing enzyme；EC1.6, including act on NADH or NADPH oxidoreducing enzyme；EC1.7, including act on as donor The oxidoreducing enzyme of other nitrogen-containing compounds；EC1.8, including act on the oxidoreducing enzyme of the methylthio group of donor；EC1.9, bag Include the oxidoreducing enzyme for the heme group for acting on donor；EC1.10, including act on diphenol and correlative as donor The oxidoreducing enzyme of matter；EC1.11, including act on the oxidoreducing enzyme of the peroxide as acceptor, also referred to as peroxide Enzyme；EC 1.12, including act on the oxidoreducing enzyme of the hydrogen as donor；EC 1.13, including act on single donor and simultaneously Enter the oxidoreducing enzyme of molecular oxygen, also referred to as oxygenase；EC 1.14, including act on the donor of pairing and be incorporated to molecular oxygen Oxidoreducing enzyme；EC 1.15, including act on the oxidoreducing enzyme of the superoxide radical as acceptor；EC 1.16, bag Include the oxidoreducing enzyme of oxidizing metal ions；EC1.17, including act on the oxidoreducing enzyme of CH or CH2 groups；EC 1.18, Oxidoreducing enzyme including acting on the iron-sulfur protein as donor；EC 1.19, including act on yellow as the reproducibility of donor The oxidoreducing enzyme of plain oxygen also albumen；EC 1.20, including the phosphorus or the oxidoreducing enzyme of arsenic acted in donor；EC 1.21, Including acting on X-H and Y-H to form the oxidoreducing enzyme of X-Y keys；With the EC 1.97 for including other oxidoreducing enzyme).These Some in oxidoreducing enzyme can use NADP or NAD+ as co-factor, and therefore be referred to as NAD+/NADP+ redox Enzyme.

Referred to according to the term " source of carbon ", " carbon source " or " carbon substrate " of the present invention by microbial metabolism and containing extremely Any carbon source of a few carbon atom.Carbon source may originate from renewable raw materials.Renewable raw materials are defined as needed for some industrial process The raw material wanted, it can regenerate in of short duration delay and allow it to change into desired product in enough amounts.Plant Biomass is a kind of interesting renewable carbon source (through processing or untreated).In the context of the present invention, xylose is Carbon source, and the advantageously carbohydrate of the carbon source in addition to xylose.

Term " carbohydrate " refer herein to by microbial metabolism and containing at least one carbon atom, two Any carbon source of hydrogen atom and an oxygen atom.The carbohydrate of the present invention is preferably selected from the following group：Monose such as glucose, fruit Sugar, mannose, galactolipin etc., disaccharides such as sucrose, cellobiose, maltose, lactose etc., oligosaccharides such as gossypose, stachyose, malt Magma essence etc., and polysaccharide cellulose, hemicellulose, starch etc., methanol, formaldehyde and glycerine.According to the preferred reality of the present invention Scheme is applied, the carbon source in addition to xylose advantageously includes the carbohydrate of 3,6 or 12 carbon atoms, or its any combinations. In the further preferred embodiment of the present invention, the carbon source in addition to xylose is selected from glycerine, glucose, galactolipin, fructose, breast Sugar, maltose, sucrose and its any combinations.It is highly preferred that the carbon source in addition to xylose is selected from glycerine, glucose, sucrose and its appointed What is combined, and most preferably glucose.

In the first aspect of the present invention, the present invention relates to microorganism, the microorganism is genetically modified and by by xylose BT is changed into produce 2,4- dihydroxy butyric acid, is used for wherein the microorganism is further genetically modified：

I) 1,2,4- butantriols are oxidized to 2,4- dihydroxy butyraldehydes；With

Ii 2,4- dihydroxy butyraldehydes) are oxidized to 2,4- dihydroxy butyric acid.

It is highly preferred that oxidation step i) and ii) relate separately to that BT exclusively is changed into 2,4- dihydroxies Base butyraldehyde and 2,4- dihydroxy butyraldehydes are changed into 2,4- dihydroxy butyric acid.In other words, it is therefore particularly preferred that by 1,2,4- fourth During triol is oxidized to 2,4- dihydroxy butyraldehydes and 2,4- dihydroxy butyraldehydes are oxidized into 2,4-DHB, microorganism does not produce Metabolic Intermediate is produced with optimizing 2,4-DHB.Such exclusiveness conversion can be realized by using the enzyme being described further below.

According to preferred embodiment, genetic modification i) is Coding Effects in the oxidoreducing enzyme of the CH-OH groups of donor The overexpression of at least one gene of (EC1.1 enzymes), and/or genetic modification ii) it is Coding Effects in the aldehyde radical or oxo of donor The overexpression of at least one gene of the oxidoreducing enzyme (EC1.2 enzymes) of group.

The example for acting on the oxidoreducing enzyme of the CH-OH groups of donor includes but is not limited to EC 1.1.1 enzymes and (uses NAD+ Or oxidoreducing enzyme of the NADP+ as acceptor, also referred to as NAD+/NADP+ oxidoreducing enzyme), EC 1.1.2 enzymes (use cytochromes Oxidoreducing enzyme as acceptor), EC 1.1.3 enzymes (oxidoreducing enzyme of acceptor is used as by the use of oxygen), EC 1.1.4 enzymes are (with two sulphur Oxidoreducing enzyme of the compound as acceptor), EC1.1.5 enzymes (being used as the oxidoreducing enzyme of acceptor by the use of quinone or similar compound), EC 1.1.98 enzyme (with the oxidoreducing enzyme of other known acceptor) and EC 1.1.99 enzymes (with the oxidoreducing enzyme of other acceptors).

The example for acting on the aldehyde radical of donor or the oxidoreducing enzyme of oxo group includes but is not limited to EC1.2.1 enzymes (use Oxidoreducing enzyme of the NAD+ or NADP+ as acceptor, also referred to as NAD+/NADP+ oxidoreducing enzyme), EC1.2.2 enzymes (use cell Oxidoreducing enzyme of the pigment as acceptor), EC 1.2.3 enzymes (oxidoreducing enzyme of acceptor is used as by the use of oxygen), EC 1.2.4 enzymes (use Oxidoreducing enzyme of the disulphide as acceptor), EC 1.2.5 enzymes (be used as the redox of acceptor by the use of quinone or similar compound Enzyme), EC 1.2.7 (oxidoreducing enzyme of acceptor is used as by the use of iron-sulfur protein) and EC 1.2.99 enzymes (with the oxidations of other acceptors also Protoenzyme).

Show that above-mentioned active enzyme is well known in the present art, and can easily be identified by those skilled in the art, Such as identified from publicly available database such as BRENDA.

In order to which BT is oxidized into 2,4- dihydroxy butyraldehydes, the oxidation of CH-OH groups of donor is acted on also Protoenzyme (EC1.1 enzymes) is preferably selected from the following group：Alcohol dehydrogenase (i.e. aldehyde reductase), aldehyde dehydrogenase, lactaldehyde reductase, glyoxalic acid reduction Enzyme, two dehydrogenation gluconic acid reductases and its any combinations.It is highly preferred that the EC1.1 oxidoreducing enzyme is alcohol dehydrogenase (i.e. aldehyde Reductase) or lactaldehyde reductase.It is more preferred still that the EC1.1 oxidoreducing enzyme is the CH-OH groups for acting on donor NAD+/NADP+ oxidoreducing enzyme (i.e. EC1.1.1 enzymes), or with other receptor actings in the redox of the CH-OH groups of donor Enzyme (i.e. EC1.1.99 enzymes).Most preferably, the enzymes of EC 1.1 are EC 1.1.1 enzymes.EC1.1 of the selection suitable for the object of the invention Enzyme and identify its corresponding gene order in the limit of power of those skilled in the art.

The particularly preferred oxidoreducing enzyme (EC1.1 enzymes) of the CH-OH groups of donor is acted on according to it in database Sequence identifier and accession number described in following table 1：Alcohol dehydrogenase includes but is not limited to sequence SEQ ID NO:1 to SEQ ID NO:40；Lactaldehyde reductase includes but is not limited to sequence SEQ ID NO:41 enzyme；Glyoxylate reductase includes but is not limited to Sequence SEQ ID NO:42 and SEQ ID NO:43 enzyme, and two dehydrogenation gluconic acid reductases include but is not limited to sequence SEQ ID NO:44 and SEQ ID NO:45 enzyme.

In particularly preferred embodiment of the invention, 2 are changed into according to BT as shown in Figure 1, The conversion metabolic pathway of 4- dihydroxy butyraldehydes, the oxidoreducing enzyme (EC1.1 enzymes) for acting on the CH-OH groups of donor is sequence SEQ ID NO:36 or SEQ ID NO:41 enzyme.

The gene for encoding oxidoreducing enzyme mentioned above is also known in the art, and is retouched in following table 1 State：

The gene of-coding alcohol dehydrogenase includes but is not limited to bacillus coli gene frmA (also referred to as adhC), adhP (also referred to as For yddN), yiaY, eutG (also referred to as yffV), yqhD, yeaE, ydhF, yhdN, ybdR, ybdH, ybjJ, ydjL, ahr, YahK, tas (also referred to as ygdS) and gldA；Acetone-butanol clostridium gene bdhB, bdhA and CA_C3392, genes of brewing yeast ADH1, ADH2, ADH3, ADH4 and bdh1；Bacillus subtilis genes yhdN and bdhA；Gluconobacter oxydans (Gluconobacter oxydans) gene GOX1615；Arabidopsis gene AKR4C9；Leishmania donovani (Leishmania Donovani) Gene A 4UTP6；The hot anaerobic bacillus(cillus anaerobicus) of producing and ethanol (Thermoanaerobacter ethanolicus) gene adh A； Corynebacterium glutamicum (Corynebacterium glutamicum) gene butA and budC；Friedlander's bacillus (Klebsiella pneumoniae) gene budC；Serratia marcescens (Serratia marcescens) gene slaC；It is red Brown meat seat bacterium (Hypocrea jecorina) gene gld2；Clostridium butyricum (Clostridium butyricum) gene dhaT； Citrobacter freundii (Citrobacter Freundii) gene dhaT；Friedlander's bacillus (Klebsiella Pneumoniae) gene dhaT；With the Rhodococcus erythropolis (Rhodoccus of coding NMDA dependence alcohol dehydrogenase Erythropolis) gene；With the methanol amycolatosis (Amycolatopsis of coding NMDA dependence alcohol dehydrogenase Methanolica) gene；

The gene of-coding lactaldehyde reductase includes but is not limited to bacillus coli gene fucO；

The gene of-encoding glyoxylate acid reductase includes but is not limited to bacillus coli gene ghrA (also referred to as ycdW) and ghrB (also referred to as yiaE)；With

The gene of two dehydrogenation gluconic acid reductases of-coding includes but is not limited to bacillus coli gene dkgA (also referred to as yqhE) With dkgB (also referred to as yafB).

In a particularly preferred embodiment of the present invention, the oxidoreducing enzyme of the CH-OH groups of donor is acted on (EC1.1 enzymes) is by the fucO genes from Escherichia coli and/or from clostridium butyricum, citrobacter freundii or kerekou pneumonia primary The dhaT gene codes of Salmonella, i.e., the conversion for being changed into 2,4- dihydroxy butyraldehydes according to BT as shown in Figure 1 are metabolized way Footpath.

As indicated above, microorganism of the invention is also genetically modified to be used for ii) 2,4- dihydroxy butyraldehydes are oxidized to 2, 4- dihydroxy butyric acid.Therefore, redox of the Coding Effects in the aldehyde radical or oxo group of donor can be overexpressed in microorganism At least one gene of enzyme (EC1.2 enzymes).

Therefore, the oxidoreducing enzyme (enzymes of EC 1.2) of the aldehyde or oxo group that act on donor is preferably selected from the following group：Aldehyde takes off Hydrogen enzyme, aldehyde oxidase and its any combinations.It is highly preferred that the EC1.2 oxidoreducing enzyme is aldehyde dehydrogenase.It is more preferred still that The EC1.2 oxidoreducing enzyme be the NAD+/NADP+ oxidoreducing enzyme of the aldehyde radical or oxo group that act on donor (i.e. EC1.2.1 enzymes), or by the use of oxygen as receptor acting in the aldehyde radical of donor or oxidoreducing enzyme (the i.e. EC 1.2.3 of oxo group Enzyme).Most preferably, the enzymes of EC 1.2 are EC 1.2.1 enzymes.Selection is suitable to the EC1.2 enzymes of the object of the invention and identifies that its is corresponding Gene order in the limit of power of those skilled in the art.

Act on donor aldehyde radical or oxo group particularly preferred oxidoreducing enzyme (enzymes of EC 1.2) according to it in number According to the sequence identifier in storehouse and accession number described in following table 1：Aldehyde dehydrogenase includes but is not limited to sequence SEQ ID NO: 46 to SEQ ID NO:60 enzyme；Aldehyde oxidase includes but is not limited to sequence SEQ ID NO:61 enzyme.

In particularly preferred embodiment of the invention, 2,4- dihydroxy butyraldehydes are changed into 2 according to as shown in Figure 1, The conversion metabolic pathway of 4- dihydroxy butyric acid, the oxidoreducing enzyme (EC1.2 enzymes) for acting on donor aldehyde radical or oxo group is sequence Arrange SEQ ID NO:46、SEQ ID NO:54 or SEQ ID NO:55 enzyme.

The gene of-encoding aldehyde dehydrogenase includes but is not limited to bacillus coli gene puuC, sad, gabD, prr (also referred to as YdcW), aldA, aldB (also referred to as yiaX), feaB and astD；Friedlander's bacillus KPN_01018；Genes of brewing yeast ALD4 and ALD5；Pseudomonas putida (Pseudomonas putida) gene davD；Pseudomonas strain gene amnC；With Methanococcus jannaschii (Methanocaldococcus jannaschii) gene M J1411；

The gene of-coding dioxovalerate dehydrogenase includes but is not limited to bacillus licheniformis gene ycbD；With

The gene of-encoding aldehyde oxidase includes but is not limited to maize Gene A O1.

In a particularly preferred embodiment of the present invention, the oxidation of aldehyde radical or oxo group of donor is acted on also Protoenzyme (EC1.2 enzymes) is by aldA, aldB or puuC gene code from Escherichia coli, i.e., according to as shown in Figure 1 by 2,4- Dihydroxy butyraldehyde is changed into the conversion metabolic pathway of 2,4- dihydroxy butyric acid.

It must further be noted that it is already present on when encoding above-mentioned active endogenous gene in microorganism interested When, they are advantageously overexpressed in the microorganism.On the contrary, when microorganism is not natively comprising the such active gene of coding When, advantageously with one or more gene transformation microorganisms for encoding the enzyme：Think also to be overexpressed the foreign gene. As explained above, foreign gene is preferably and has carried out codon optimization to be used for its synthesis base expressed in microorganism interested Cause.

In the context of the present invention, microorganism can be by xylose into BT.It is genetically modified to be used for Microorganism by xylose into BT, particularly for it is exclusive by xylose into BT Microorganism is it is known in the art that and being retouched especially through patent application WO2008/091288 and US2013/0203141 State, it is incorporated herein by carrying stating.

Therefore, in a preferred embodiment, for being by genetic modification of the xylose into BT The overexpression of at least one following gene：

The gene of-coding xylose dehydrogenase,

The gene of-coding xylonolactonase,

The gene of-coding xylonate dehydratase,

The gene of-coding 3- deoxidations-D- glycerine-pentanone saccharic acid (DGP) decarboxylase,

The gene of-coding BT dehydrogenase, and

- its any combinations.

The gene for encoding enzyme described above is well known in the present art：

The gene of-coding xylose dehydrogenase is disclosed in US2013/0203141 and WO2008/091288, is stated simultaneously by carrying Enter herein, and include but is not limited to from crescent shank bacterium (Caulobacter crescentus), dead sea salts box bacterium (Haloarcula marismortui), fungi bulkholderia cepasea (Burkholderia fugorum) LB400 and walsh Rich salt bacterium (Haloferax volcanii) DS2 xdh encoding genes,

The gene of-coding xylonolactonase includes but is not limited to the xylC genes from crescent shank bacterium,

Disclosed in US2013/0203141 and WO2008/091288, it passes through the gene of-encoding D-xylose acid dehydratase Carry stating and be incorporated herein, and include but is not limited to yjhG the and yagF genes from Escherichia coli,

The gene of-coding 3- deoxidations-D- glycerine-pentulose acid decarboxylase is disclosed in US2013/0203141 and WO2008/ In 091288, it is incorporated herein by carrying stating, and includes but is not limited to the mdlC genes from pseudomonas putida, and

The gene of-coding NADPH dependence 1,2,4- butantriol dehydrogenases is disclosed in US2013/0203141 and WO2008/ In 091288, it is incorporated herein by carrying stating, and includes but is not limited to adhP the and yqhD genes from Escherichia coli.

In a preferred embodiment, particularly when genetically modified microorganism is Escherichia coli：

α) gene for encoding xylose dehydrogenase, xylonolactonase and 3- deoxidations-D- glycerine-pentulose acid decarboxylase is to lead Enter the heterologous gene in microorganism, and

It is endogenous gene β) to encode xylonate dehydratase and the gene of BT dehydrogenase, therefore advantageously It is overexpressed.

In a particularly preferred embodiment of the present invention, xylose dehydrogenase is compiled by the xdh genes of crescent shank bacterium Code, xylonolactonase by crescent shank bacterium xylC gene codes, xylonate dehydratase by the yjhG from Escherichia coli and/ Or yagF gene codes, 3- deoxidations-D- glycerine-pentulose acid decarboxylase by the mdlC gene codes from pseudomonas putida, And BT dehydrogenase is by adhP the and/or yqhD gene codes from Escherichia coli, i.e., according to as shown in Figure 1 Xylose is changed into the conversion metabolic pathway of 1,2,4- butantriols.

The nucleotides of gene mentioned above or by the gene code amino acid sequence according to them in database Accession number and version and/or according to their sequence identifier described in following table 1.

When the above-mentioned active endogenous gene of coding is already present in microorganism interested, they are advantageously described It is overexpressed in microorganism.On the contrary, when microorganism natively comprising such active gene is encoded when, advantageously described in coding One or more gene transformation microorganisms of enzyme：Think also to be overexpressed the foreign gene.As explained above, foreign gene The synthetic gene that codon optimization is used to express in microorganism interested is preferably carried out.

According to a preferred embodiment, microorganism of the invention is further genetically modified to be used for：

Iii) reducing power for the production of 2,4- dihydroxy butyric acid and microorganism growth is provided from the carbon source in addition to xylose And/or energy, and/or

Iv) at least in part, carbon catabolite repression is preferably entirely suppressed.

Genetic modification iii as described above) it is particularly advantageous, because it optimizes 2,4-DHB productions, by using Alternative carbon source rather than xylose provide reducing power and/or energy, so as to which xylose is exclusively changed into 2,4- dihydroxy Butyric acid.

In order to provide the reducing power and/or energy of the production of 1,2,4- butantriols and microorganism growth from the carbon source in addition to xylose Amount, preferably encoded by lacking and/or weakening using the gene of xylose or the enzyme of other metabolins come genetic modification according to this hair Bright microorganism, the metabolin produces is changed into the flow (flux) that the conversion of 2,4- dihydroxy butyric acid mutually competes with xylose.

Therefore, according to a preferred embodiment of the invention, genetic modification iii) it is at least one in following gene Reduction and/or missing：

The gene (such as xylA genes from Escherichia coli) of-encoding xylose isomerase,

The gene (such as xylB genes from Escherichia coli) of-encoding xylulokinase,

- coding 3- deoxidations-D- glycerine-pentulosonate aldolase gene (such as yjhH from Escherichia coli and/or YagE genes),

The gene (such as yiaE and/or ycdW genes from Escherichia coli) of-coding ketoacid dehydrogenase, and

- its any combinations.

These genes and fully disclosed in US2013/0203141 for lacking or weakening their method, by carrying State and be incorporated herein.

The nucleotides of gene mentioned above or by the gene code amino acid sequence also according to them in database In accession number and version and/or according to their sequence identifier described in following table 1.

If the microorganism of the present invention carries out genetic modification as described above, accordingly preferably microorganism is used except wood Carbon source outside sugar produces and reducing power and/or energy necessary to microorganism growth to provide 2,4-DHB.In this respect, at this It is well known that, some microbials are for other carbon sources preferably some specific carbon sources in field.It is it is worth noting that, most of Naturally occurring microorganism, particularly Escherichia coli preferably use glucose relative to other sugar, that is, allow them to metabolism one Serial monose (Kim etc., 2010).However, some microorganisms can not utilize glucose and xylose altogether in an efficient way.It is actual On, when two kinds of sugar are present in culture medium, the second stage of (dizuxic) fermentation pattern can occur in microorganism：This regulation mechanism exists Known in the art is catabolite repression, and glucose and lactose have been reported by Monod in the 1940s. Through widely studied such mechanism in enterobacteriaceae.In Escherichia coli, substantially recognize that catabolite repression is as follows Occur：Glucose enters phosphoenolpyruvate (PEP) by glucose specificity permease EIICBglc (being encoded by ptsG): Carbohydrate phosphotransferase system (PTS)；During glucose transport, (compiled by dephosphorylized EIIAglc by crr Code) ring AMP (cAMP) level is reduced, this in turn limits catabolite repression albumen (CRP) and cAMP compounds (cAMP-CRP) availability；Then, it is related to the catabolism of carbohydrate in addition to glucose and the institute generally adjusted by cAMP-CRP There is gene all to be prevented.Also do not reported in the literature by the catabolite repression of the carbohydrate of PTS system transhipment, but Not yet it is fully understood (Hogema etc., 1997).

Therefore, in a preferred embodiment, it is allowed to suppress the genetic modification iv of carbon catabolite repression) it is selected from Following at least one：

The gene of the glucose permease of-missing coding phosphotransferase system,

The gene of-missing coding phosphate carrier Hpr albumen,

- preferably never involved in by the composition or the sugared input albumen system of inducible promoter expression of cAMP-CRP regulations And gene and/or operator, wherein the sugar is the carbon source in addition to xylose,

- expression encodes xylose transport albumen, such as the gene of transport protein in the same direction or abc transport albumen, preferably never by The composition of cAMP-CRP regulations or inducible promoter expression,

- gene for encoding glucose symport albumen is overexpressed,

- gene for encoding facilitated glucose albumen is overexpressed,

- gene for encoding glucokinase is overexpressed,

- regulation involves the horizontal genes of cAMP, the expression of the gene of optimized encoding adenyl cyclase,

The expression of the gene of-regulation coding CRP and/or CRP sample protein,

- expression encodes the gene of CRP protein independent of cAMP, preferably never by cAMP-CRP regulations composition or Inducible promoter is expressed, and

- its any combinations.

It is highly preferred that utilize xylose and glucose, missing coding phosphotransferase and/or phosphate carrier Hpr eggs in order to common White gene advantageously encodes Portugal with being overexpressed the gene of coding glucose permease or facilitated glucose albumen together with overexpression The assortment of genes of sugared kinases.

The gene for encoding above-described protein is well known in the art：

The gene of the glucose permease of-coding phosphotransferase system includes but is not limited to the pstG from Escherichia coli Gene,

(it is phosphoenolpyruvate to-coding phosphate carrier Hpr protein：The two of sugar phosphotransferase system (PTS sugar) One of kind sugared nonspecific protein component) gene include but is not limited to the ptsH genes from Escherichia coli,

- be related to sugared input albumen system (PTS or other systems) gene/operator include but is not limited to come from large intestine bar Bacterium be used for assimilate lactose lacY genes, from Escherichia coli be used for assimilate maltose malFG genes and respectively come From scrKYABR the or cscBKAR operators for being used to assimilate sucrose of salmonella typhimurium and Escherichia coli,

The gene of-coding xylose transport albumen includes but is not limited to the xylFGH operators from Escherichia coli, comes arrogant XylE the and araE genes of enterobacteria,

The gene of-coding glucose symport albumen includes but is not limited to the galP genes from Escherichia coli,

The gene of-coding facilitated glucose albumen includes but is not limited to zymomonas mobilis (Zymomonas Mobilis glf genes),

The gene of-coding glucokinase includes but is not limited to the glk genes from Escherichia coli,

The gene of-coding adenyl cyclase includes but is not limited to the cyaA genes from Escherichia coli,

The gene of-coding CRP and/or CRP sample protein includes but is not limited to the CRP genes from Escherichia coli and come from Bacillus subtilis and the ccpA genes of other Firmacuteses (firmicutes),

Gene of the coding independent of cAMP CRP protein has been described in-Hogema etc. (1997), includes but is not limited to Encode the gene for the CRP albumen being mutated in Liang Ge areas：Residue 53 to 62 corresponding to the cAMP binding domain of CRP albumen and correspondingly In the residue 141 to 148 of the proximal boundary of DNA binding domain (helix turn helix).The example bag of such protein mutant CRP mutant Asp is included to His residues 53, Ser to Phe residues 62, Gly to Asp residues 141, Arg to Asp residues 142, Leu To Arg residues 148 and its any combinations.

It is highly preferred that in order to utilize xylose and glucose altogether in Escherichia coli, ptsG or ptsH gene delections can be with The overexpression of galP or glf genes combines together with the overexpression of glk genes.

The nucleotide sequence of gene mentioned above or by the gene code amino acid sequence according to them in database In accession number and version and/or according to their sequence identifier described in following table 1.

As explained above, when the above-mentioned active endogenous gene of coding is already present in microorganism interested, they Advantageously it is overexpressed in the microorganism.However, if microorganism has not natively comprising such active gene is encoded Sharply with one or more gene transformation microorganisms for encoding the enzyme：Think also to be overexpressed the foreign gene.As above Text explains that foreign gene is preferably to have carried out the synthetic gene that codon optimization is used to express in microorganism interested.Phase Instead, endogenous gene can be lacked according to above-described any method or weakens its expression.

It is as defined above according to the carbon source in addition to xylose of the present invention, and include 3,6 or 12 carbon The carbohydrate of atom, or its any combinations.Preferably, the carbon source in addition to xylose is selected from the group：Glycerine, glucose, gala Sugar, fructose, lactose, maltose, sucrose and its any combinations.

Most preferably, the carbon source in addition to xylose used in the present invention is glucose.

Or the carbon source in addition to xylose can be sucrose.Therefore, in a preferred embodiment of the invention, microorganism includes To use sucrose, as the carbon source in addition to xylose, as described in WO2012/004247, it is stated further genetic modification by carrying It is incorporated herein.Therefore, microorganism preferably comprises the function base that coding PTS sucrose utilizes system using system and/or non-PTS sucrose Cause.

PTS sucrose is based upon phosphoenolpyruvate (PEP)-dependence sucrose phosphate using system and shifts enzyme system The sucrose of (sucrose-PTS) transhipment sucrose utilizes system.Phosphotransferase system by the transhipment of sugared (such as sucrose or glucose) with Sugared phosphorylation coupling using PEP as phosphodonor.After cell is transported, sucrose phosphate is converted digestion and is cut into grape Sugar -6- phosphoric acid and fructose.Fructose then by fructokinase phosphorylation into fructose-6-phosphate.This PTS sucrose is encoded using system Gene can be controlled by regulatory protein.

Non- PTS sucrose is based upon transporting the sucrose profit of sucrose independently of the system of phosphoenolpyruvate using system Use system.After cell is transported, sucrose is converted digestion and is cut into glucose and fructose.Fructose is then by fructokinase phosphorylation Into fructose-6-phosphate, and glucose is melted into G-6-P by glucokinase phosphoric acid.Encode this non-PTS sucrose and utilize system Gene can be controlled by regulatory protein.

In a particularly preferred embodiment of the present invention, microorganism is naturally expressed or genetically modified with table Up to the gene of the operator scrKYABR from Salmonella, i.e. the scrK genes of encoding fructose kinases, coding PFP ScrY genes, encode the scrA genes of IIBC albumen, the scrB genes of encoding sucrose -6-P invertases and coding repressor ScrR genes, and any combination of them.The conjugative plasmid pUR400 microbials for carrying scrKYABR can be used.These Gene individually, with least two any combinations comprising these genes or whole combine can express in microorganism. Preferably omit gene scrR.

In particularly preferred embodiment of the invention, microorganism naturally express or it is genetically modified with express come From coli strain EC3132 gene, i.e. encoding sucrose:Proton transports movement system (cscB genes), fructokinase in the same direction (cscK), the operator cscBKAR genes of invertase (cscA genes) and sucrose specificity repressor (cscR genes).These bases Cause individually, with least two any combinations comprising these genes or whole combine can be expressed in microorganism.Can It is preferred that omit gene cscR.It can also be used from other biological homologous genes.

However, in a preferred embodiment, microorganism of the invention, which includes to involve, produces NADPH as also proper energy The further genetic modification of at least one gene in power source.In fact, reductase such as dehydrogenase need it is available in microorganism Reducing power, particularly in the form of NADPH.The strategy of NADPH availabilities is well known in the present art in increase cell, and And especially summarized by Lee etc. (2013), and also retouched by US8088620, WO2012/055798 and EP14305691.9 State, above-mentioned file is incorporated herein by carrying stating.

According to the present invention, for improving NADPH generations and therefore improving the genetic modification of its availability in microorganism It is preferably selected from：

- gene or operator for encoding membrane-bound transhydrogenase are overexpressed,

The gene of-missing or reduction encoding soluble transhydrogenase,

- gene that coding produces NADPH glyceraldehyde-3-phosphate dehydrogenase is overexpressed,

The gene of-missing or reduction coding phosphogvlucoisomerase,

The gene of-missing or reduction coding phosphofructokinase,

- gene for encoding glucose-6-phosphate dehydrogenase (G6PD) is overexpressed,

- mutant gene that coding can produce NADPH diaphorase is overexpressed,

- gene for encoding difunctional NAD (P) H hydrate repair enzymes is overexpressed, and

- its any combinations.

The gene of missing or reduction coding phosphofructokinase more preferably encodes glucose-6-phosphate dehydrogenase (G6PD) with being overexpressed The assortment of genes, to increase by the NADPH flows of pentose phosphate pathway.

It is highly preferred that it is selected from for improving genetic modification caused by NADPH：

- gene for encoding membrane-bound transhydrogenase is overexpressed,

- missing or the gene of reduction coding phosphogvlucoisomerase and/or soluble transhydrogenase, and

- it is overexpressed the gene that coding produces NADPH glyceraldehyde-3-phosphate dehydrogenase.

The gene for encoding above-described protein is well known in the present art：

The gene or operator of-coding membrane-bound transhydrogenase include but is not limited to the pntAB operators from Escherichia coli, Especially as described in WO2012/055798A1,

The gene of-encoding soluble transhydrogenase includes but is not limited to the udhA genes from Escherichia coli,

The gene that-coding produces NADPH glyceraldehyde-3-phosphate dehydrogenase includes but is not limited to from Streptococcus mutans GapN (such as Centeno-Leija, described in 2013), it can be used for for example substituting the endogenous gapA gene from Escherichia coli,

The gene of-coding phosphogvlucoisomerase includes but is not limited to the pgi genes from Escherichia coli,

- coding phosphofructokinase gene include but is not limited to the pfkA genes from Escherichia coli, such as especially by WO2005/047498 is described,

The gene of-coding glucose-6-phosphate dehydrogenase (G6PD) includes but is not limited to the zwf genes from Escherichia coli, such as special Do not described by Lim etc. (2002),

The mutant gene that-coding can produce NADPH diaphorase includes but is not limited to come from large intestine bar The mutation lpd genes (lpd*) of bacterium, such as especially described by Bocanegra (1993), and

The gene of difunctional NAD (P) the H hydrate repair enzymes of-coding includes but is not limited to the yjeF bases from Escherichia coli Cause, such as especially described by Marbaix (2011).

In Escherichia coli, if missing or reduction pfkA genes, the genetic modification is preferably with being overexpressed zwf genes Combination.

- the pntAB operators from Escherichia coli are overexpressed,

PfkA gene and/or udhA gene of-the missing from Escherichia coli,

- with from Streptococcus mutans gapN genes substitute the gapA from Escherichia coli.

The nucleotide sequence of gene mentioned above or by the gene code amino acid sequence according to them in data Accession number and version in storehouse and/or according to their sequence identifier described in following table 1.

Table 1：The gene and protein of the present invention (ND=is undisclosed)

On the other hand, the present invention relates to the method for production 2,4- dihydroxy butyric acid, it includes：

A) in the culture medium comprising xylose, allowing to train under fermentation condition of the xylose into 2,4- dihydroxy butyric acid The genetically modified microorganism of invention as described above is supported, and

B) 2,4- dihydroxy butyric acid is reclaimed from the culture medium.

Fermentation medium and carbon source are as known in the art.According to the present invention, term " fermentation process ", " fermentation " or " culture " is used interchangeably, and refers to the experiment condition for allowing given microorganism growth.The growth of microorganism is generally with suitable for institute Carried out with the fermentation tank of the appropriate growth medium of microorganism.

" appropriate culture medium " refers to culture medium (such as aseptic liquid nutrient medium) herein, and it is included for microorganism Maintain and/or grow required or beneficial nutrients such as carbon source or carbon substrate；Nitrogen source, such as peptone, yeast extract, meat Extract, malt extract, urea, ammonium sulfate, ammonium chloride, ammonium nitrate and ammonium phosphate；Phosphorus source, such as potassium dihydrogen phosphate or phosphoric acid Hydrogen dipotassium；Micro- (such as metal salt), such as magnesium salts, cobalt salt and/or manganese salt；And growth factor such as amino acid and Wei Sheng Element.

According to a preferred embodiment, for step a) culture medium further comprising the carbon source in addition to xylose, its is preferred For carbohydrate.

The carbohydrate may be selected from the following group：Monose such as glucose, fructose, mannose, arabinose, galactolipin etc., Disaccharides such as sucrose, cellobiose, maltose, lactose etc., oligosaccharides such as gossypose, stachyose (stacchyose), maltodextrin Deng, polysaccharide such as cellulose, hemicellulose, starch etc., methanol, formaldehyde and glycerine.According to the particularly preferred carbon aquation of the present invention Compound includes 3,6 or 12 carbon atoms, more preferably glycerine, glucose, fructose, galactolipin, lactose, maltose, sucrose and its appoints What is combined.Most preferably, carbohydrate is glucose.Or the carbohydrate can be sucrose.

In a preferred embodiment of the invention, carbon source (preferably carbohydrate) is derived from renewable raw materials, such as plants Thing biomass.

Those skilled in the art can be readily determined for cultivating according to condition of culture necessary to microorganism of the invention.It is special Not, it is known that can be at 20 DEG C -55 DEG C, the preferably temperature at 25 DEG C -40 DEG C makes bacterial fermentation.More particularly, can be about 30 DEG C to about 37 DEG C of temperature culture Escherichia coli.

The method of the present invention can be with batch process, fed batch process or continuous process and in aerobic, micro- aerobic or anaerobism Under the conditions of carry out.

" under aerobic conditions " fermentation means by being supplied to oxygen in the liquid phase that dissolves gas into culture Culture.This can be achieved by the following procedure：(1) oxygen-containing gas (such as air) is sprayed into liquid phase, or (2) are shaken containing culture The container of base is so that oxygen contained in headroom is transferred in liquid phase.The major advantage fermented under aerobic conditions is that oxygen is made Improve ability of the energy of the more ATP forms of bacterial strain generation for cell processes for the presence of electron acceptor, thus improve bacterial strain General metabolism.

Micro- aerobic condition can be used herein and is defined as the oxygen of low percentage (for example, using containing 0.1- 10% oxygen, filled with nitrogen to 100% admixture of gas) it is dissolved into the condition of culture in liquid phase.

On the contrary, " anaerobic condition " is defined as not providing the condition of culture of oxygen to culture medium.Strictly anaerobic condition can pass through Inert gas (such as nitrogen) is sprayed into culture medium to remove other gases of trace to obtain.Nitrate can be used as electron acceptor Produce to improve the ATP of bacterial strain and improve its metabolism.

According to preferred embodiment, the method for the invention also step c) of 2, the 4-DHB including purification step b).For The method of purification of carboxylic acids and specifically carboxylic acid is well known in the present art, and especially WO2002/090312, Described in WO2002/022544 and WO2012/153042, it is incorporated herein by carrying stating.Preferably, people in the art is passed through Means known to member, and particularly ion-exchange chromatography such as ion exchange resin or fixation or Simulation moving bed ion exchange Resin, 2,4-DHB is purified after it is reclaimed in culture medium.

Brief description

Fig. 1 represents the metabolic pathway (* by D- xyloses into 2,4-DHB：The gene for optionally lacking or weakening).

Embodiment

The present invention is further defined in the examples below.It should be understood that although these embodiments indicate this The preferred embodiment of invention, but only provide by way of illustration.From disclosure above and these examples, the technology of this area Personnel can carry out various change to be adapted to various uses and condition to the present invention, the basic hand without changing the present invention Section.

Describe the Exemplary gene for building Institute of Micro-biology's needs with these abilities and enzyme and for cloning and turning Change, the method that monitoring product is formed and produced using engineered microorganisms.

Specifically, embodiment shows the coli strain through modification, but these modifications can be easily mutually equal Other microorganisms in carry out.

Escherichia coli belong to enterobacteriaceae, and it includes Gram-negative, shaft-like, non-sporogenesis, and length is usually 1-5 μm of member.Most of members have the flagellum for activity, but some category are not movable.Many members of this section The normal segments of the gut flora found in the intestines of the mankind and other animals, and it is other found in water or in soil, or Person is the parasitic animal and plant on a variety of different animals and plant.Escherichia coli are one of most important model organisms, but enterobacteriaceae Other important members include Klebsiella (particularly Klebsiella terrigena, plant Klebsiella or the sour Cray of production Primary Salmonella), general Pseudomonas and Salmonella.

Scheme

Several schemes for building 2,4- dihydroxy butyric acid production bacterial strain are described in following examples.

Use in the present inventionScheme 1(pass through the chromosome modification of homologous recombination, the selection of recombinant and antibiotic box Excision) andScheme 2(bacteriophage P1 transduction) has fully described in patent application WO2013/001055.

Scheme 3：Construction recombination plasmid

It is known to those skilled in the art to fully describe and know recombinant DNA technology.

In short, use oligonucleotides (those skilled in the art can design) and MG1655 e. coli k-12s or other Microbe genome DNA enters performing PCR amplification to DNA fragmentation as matrix (according to target gene to be amplified).With compatible limit Enzymic digestion DNA fragmentation processed and the plasmid of selection, connection, are then transformed into competent cell.Analysis transformant is simultaneously surveyed by DNA Sequence verifies recombinant plasmid interested.

Embodiment 1：By in MG1655 Escherichia coli enzyme of the excess generation from new way from xylose production 2,4- DHB- builds bacterial strain 1 to 11.

Coli strain MG1655 is modified to use the approach shown in Fig. 1 from D- xylose production 2,4- dihydroxy butyric acid (2,4-DHB).The work is sought to maximize the carbon flow towards 2,4-DHB productions, so as to remove related to other xyloses Consumption or be related to 2,4-DHB- midbody compounds conversion (which represent the loss of product) enzyme.

Except gene (the sequence SEQ ID NO naturally expressed by Escherichia coli:64 yjhG genes and sequence SEQ ID NO: 65 yagF genes, it encodes xylonate dehydratase；And sequence SEQ ID NO:66 adhP genes and sequence SEQ ID NO:67 yqhD genes, it encodes alcohol dehydrogenase-NAD (P) H dependence BTs dehydrogenase) outside, use pCL1920 Plasmid (Lerner＆Inouye, 1990), with Ptrc Artificial promoters (sequence provided in patent WO 2007/0770441) and The ribosome bind site of its own expresses the gene for encoding following enzyme respectively：The xylose dehydrogenase and xylonic of crescent shank bacterium Lactonase (is respectively the xdh [CC0821 on CauloCyc, SEQ ID NO:62] and xylC [CC0820 on CauloCyc, SEQ ID NO:63]), 3- deoxidations-D- glycerine-pentulose acid decarboxylase (sequence SEQ ID NO of pseudomonas putida:68 MdlC genes), alcohol dehydrogenase-NAD (P)+dependence 2 of Escherichia coli or clostridium butyricum, 4- dihydroxy butyraldehyde dehydrogenase gene sequences (it is respectively coded sequence SEQ ID NO:The fucO genes or coded sequence SEQ ID NO from Escherichia coli of 41 enzyme:36 Enzyme the dhaT genes from clostridium butyricum) and Escherichia coli aldehyde dehydrogenase (coded sequence SEQ ID NO:46 enzyme PuuC genes or coded sequence SEQ ID NO from Escherichia coli:The aldB genes from Escherichia coli of 55 enzyme).It is real On border, clone gene xdh, xylC, mdlC first on pCL1920 plasmids, plasmid pDHB0001 is provided, then in plasmid The upper sequential clone fucO or dhaT and puuC or aldB of pDHB0001,4 kinds of combinations are produced, obtain plasmid pDHB0002 extremely PDHB0005, described in following article table.

It is same described in 2000 using by Datsenko＆Wanner in addition, in order to block natural wood sugar decomposition metabolic pathway Source restructuring is tactful and according to scheme 1, and encoding D-xylose isomerase (sequence SEQ is lacked from Escherichia coli MG1655 chromosomes ID NO:69 xylA genes) and D- Xylulokinases (sequence SEQ ID NO:70 xylB genes) gene.More accurately, In order to lack xylAB operators, with SEQ ID NO:104 and SEQ ID NO:105 primer, which produces, carries antibiotic resistance base Because of the PCR primer surrounded by the sequence homologous with the upstream and downstream area of xylAB operators together with FRT sites, and led Enter in the previously inverted Escherichia coli MG1655 for there are a pKD46 carriers.Turned with appropriate oligonucleotides checking antibiotic resistance Change body, and be bacterial strain 1 by the Strain Designation of reservation.

In order to avoid the degraded of 3- deoxidation-D- glucose pentanone saccharic acids (DGP), also lacked using identical homologous recombination strategy Coding ketoacid dehydrogenase (sequence SEQ ID NO:73 yiaE genes；With sequence SEQ ID NO:74 ycdW genes) and DGP aldolases (sequence SEQ ID NO:71 yjhH genes；Sequence SEQ ID NO:72 yagE genes) gene.It is more accurate Ground, in order to lack yjhH genes (SEQ ID NO:71), with SEQ ID NO:106 and SEQ ID NO:107 primer is produced and taken The PCR surrounded by the sequence homologous with the upstream and downstream area of yjhH genes with antibiotics resistance gene together with FRT sites is produced Thing, and be conducted into previously conversion have in the bacterial strain 1 of pKD46 carriers.Converted with appropriate oligonucleotides checking antibiotic resistance Body, and be bacterial strain 2 by the Strain Designation of reservation.Then, in order to lack yagE genes (SEQ ID NO:72), produce and carry antibiosis Plain resistant gene together with FRT sites by with yagE genes (SEQ ID NO:108 and SEQ ID NO:109) upstream and downstream The PCR primer that the homologous sequence in area surrounds, and be conducted into previously conversion have in the bacterial strain 2 of pKD46 carriers.With appropriate few core Thuja acid verifies antibiotic resistance transformant, and is bacterial strain 3 by the Strain Designation of reservation.Then, in order to lack yiaE genes (SEQ ID NO:73), with SEQ ID NO:110 and SEQ ID NO:111 primer, which produces, carries antibiotics resistance gene together with FRT positions The PCR primer surrounded by the sequence homologous with the upstream and downstream area of yiaE genes of point, and be conducted into pre-conversion and have In the bacterial strain 3 of pKD46 carriers.Antibiotic resistance transformant is verified with appropriate oligonucleotides, and is by the Strain Designation of reservation Bacterial strain 4.Finally, in order to lack ycdW genes (SEQ ID NO:74), with SEQ ID NO:112 and SEQ ID NO:113 draw Thing, which produces, carries antibiotics resistance gene being surrounded by the sequence homologous with the upstream and downstream area of ycdW genes together with FRT sites PCR primer, and be conducted into pre-conversion and have in the bacterial strain 4 of pKD46 carriers.Resisted with appropriate oligonucleotides checking antibiotic Property transformant, and be bacterial strain 5 by the Strain Designation of reservation.

Due to yjhH genes and yjhG (SEQ ID NO:64) and yjhI belong to yjhIHG operators together, it is necessary to selection with The homologous sequence in yjhH upstream and downstream area, so as to not change the expression of surrounding genes.For belonging to yagEF operators YagE genes (SED ID NO:72) it is identical.

Finally, in order to suppress catabolite repression, by using by Datsenko＆Wanner, 2000 descriptions it is homologous Restructuring is tactful (according to scheme 1), in patent application EP 14305691.9, the institute particularly in the embodiment 2 of the document State, use SEQ ID NO:114 and SEQ ID NO:115 primer is lacked by ptsG genes (SEQ ID NO:75) Portugal of coding Grape sugar phosphotransferase IIBC (Glc).Appropriate PCR primer is imported into previously conversion has in the bacterial strain 5 of pKD46 carriers.With suitable When oligonucleotides verify antibiotic resistance transformant, and be bacterial strain 6 by the Strain Designation of reservation.

Different in kanamycins, chloramphenicol, gentamicin, tetracycline, blasticidin or spectinomycin resist is used every time Raw plain resistant gene.Before using bacterial strain 6, such as by Datsenko＆Wanner, (according to scheme 1) described in 2000, Flp is used Recombinase removes antibiotic box, producing bacterial strain 7 from Δ xylAB, Δ yjhH, Δ yagE, Δ yiaE, Δ ycdW modification.

Then, each plasmid pDHB0002, pDHB0003, pDHB0004 or pDHB0005 described above are imported into bacterial strain In 7, producing bacterial strain 8 to 11, as in the table below.

Embodiment 2：Improve 2,4- dihydroxy-butyric acid yield-structure bacterium by increasing the NADPH availabilities of production bacterial strain Strain 12 to 20.

Available reducing power in organism is needed by the adhP and yqhD BT dehydrogenases encoded, particularly NADPH forms, therefore be overexpressed and participate in gene caused by NADPH.

By the way that the endogenesis promoter of Escherichia coli MG1655 pntA genes and ribosome bind site are replaced with into induction Type Ptrc promoters (coming from plasmid pTRC99A, Amersham Pharmacia) and the ribosome bind site RBS120 determined (coming from RBS Calculator softwares), as described in patent application EP14305691.9, the embodiment 4 of particularly described document Described in (herein referred as SEQ ID NO:116) excess generation is by pntAB operators (SEQ ID NO:94) film combination of coding Proton translocation pyridine nucleotide transhydrogenase.Appropriate PCR primer described in patent application EP 14305691.9 is imported previous Conversion has in the bacterial strain 7 of pKD46 carriers.Antibiotic resistance transformant is verified with appropriate oligonucleotides, and by the bacterial strain of reservation It is named as bacterial strain 12.

Use SEQ ID NO:117 and SEQ ID NO:118 primer, by using by Datsenko＆Wanner, 2000 Particularly in the reality of the application in the homologous recombination strategy (according to scheme 1) of description and such as patent application WO2012/055798 Apply described in example 2, lack and udhA, sequence SEQ ID NO (are formerly known as by sthA genes:95) the soluble pyridine nucleosides of coding Sour transhydrogenase.Appropriate PCR primer is imported into previously conversion has in the bacterial strain 12 of pKD46 carriers.Tested with appropriate oligonucleotides Antibiotic resistance transformant is demonstrate,proved, and is bacterial strain 13 by the Strain Designation of reservation.

Use SEQ ID NO:119 and SEQ ID NO:120 primer, by using by Datsenko＆Wanner, 2000 Particularly in the reality of the application in the homologous recombination strategy (according to scheme 1) of description and such as patent application EP 14305691.9 Apply described in example 5, lack by pfkA genes (SEQ ID NO:99) phosphofructokinase of coding.Appropriate PCR primer is led In the bacterial strain 13 for entering pre-conversion pKD46 carriers.Antibiotic resistance transformant is verified with appropriate oligonucleotides, and will be retained Strain Designation be bacterial strain 14.

By using Datsenko＆Wanner, the homologous recombination strategy (according to scheme 1) of 2000 descriptions will be by from large intestine GapA genes (the SEQ ID NO of bacillus:97) the NAD dependence GAPDs of coding replace with coding NADP and relied on GapN genes (the SEQ ID NO from Streptococcus mutans of property glyceraldehyde-3-phosphate dehydrogenase:96)(Centeno-Leija Deng 2013).By the substitution for completing gapN gene pairs gapA genes as follows：GapA promoters and ribosome bind site is same When be substituted by Heat-inducible PR01 promoters (SEQ ID NO:121) and its association thermolability repressor CI857 (SEQ ID NO122) (as described in patent application EP 2532751 embodiment 1) and determine ribosome bind site RBS150 (come from RBS Calculator softwares, SEQ ID NO:123).More accurately, using SEQ ID NO:124 and SEQ ID NO:125 Primer, which produces, carries CI857 genes, PR01 promoters, RBS150 ribosome bind sites, the gapN bases from Streptococcus mutans The PCR surrounded by the sequence homologous with the upstream and downstream area of gapA genes of cause and antibiotics resistance gene together with FRT sites Product, and be conducted into and be previously converted into the bacterial strain 14 of pKD46 carriers.Turned with appropriate oligonucleotides checking antibiotic resistance Change body, and be bacterial strain 15 by the Strain Designation of reservation.

Before using bacterial strain 15, Flp recombinases are used from pntAB, udhA, pfkA and gapA locus according to scheme 1 Remove antibiotic box, producing bacterial strain 16.Finally, by each plasmid pDHB0002, pDHB0003, pDHB0004 described above or PDHB0005 is imported in bacterial strain 16, producing bacterial strain 17 to 20, as described in following table.

Embodiment 3：Excessively production is related to Sucrose Metabolism in the E. coli recombinant stain for producing 2,4- dihydroxy-butyric acid Enzyme-structure bacterial strain 21 to 26.

In order to avoid when on the mixture in sucrose and xylose cultivate bacterial strain when catabolite repression, by using by Datsenko＆Wanner, the homologous recombination strategy (according to scheme 1) of 2000 descriptions will encode the operator of the input albumen of xylose XylFGH (XylF periplasm proteins, XylG ATP binding subunits, XylH films subunit) natural promoter replaces with Artificial promoters. More accurately, using SEQ ID NO:127 and SEQ ID NO:128 primer, which produces, carries Ptrc Artificial promoters (SEQ ID NO:126) and kalamycin resistance gene together with FRT sites by with xylF genes and with the upstream of xylF genes on chromosome The PCR primer that the homologous sequence in area surrounds, and be conducted into previously conversion have in the bacterial strain 16 of pKD46 carriers.With appropriate widow Nucleotides verifies kanamycin resistant transformants, and is bacterial strain 21 by the Strain Designation of reservation.

Before using bacterial strain 21, antibiotic box, production are removed from xylFGH operators using Flp recombinases according to scheme 1 Raw bacterial strain 22.

In order to allow Escherichia coli to be grown on sucrose, by (the Schmidt of Plasmid pUR4 00 from salmonella typhimurium Deng 1982, SEQ ID NO:81) gene scrK, gene scrY, the protein of PFP (porine) of encoding fructose kinases IIBC gene scrA, the gene scrB of sucrose -6- phposphate enzymes and the gene scrR of repressor is cloned in pBBR1MCS matter Under natural promoter on grain (Kovach etc., 1995), plasmid pBDO0003 is obtained.

Finally, as in the table below, by plasmid pBDO0003 and plasmid pDHB0002, pDHB0003, pDHB0004 or One of pDHB0005 combinations introduce bacterial strain 22, producing bacterial strain 23 to bacterial strain 26.

Embodiment 4：The micro-organisms of 2,4- dihydroxy butyric acid.

Production bacterial strain is evaluated in conical flasks of the 500ml with baffle plate using the M9 culture mediums (Anderson, 1946) of improvement, The M9 culture mediums of the improvement are supplemented with 30g/l MOPS, 20g/L D- xyloses and 10g/l glucose, and are adjusted to pH 6, 8.Concentration 50mg.L-1 spectinomycin and 5mg.L-1 tetracycline are added when being necessary in preculture and culture. 37 DEG C of culture pre-culture in LB culture mediums (Sigma).After 24 hours of incubation, it is inoculated with using it in 30 DEG C and with 200rpm The 50mL cultures of the M9 culture mediums of improvement are to OD600 about 0.2.When the sugar in culture medium exhausts, culture is centrifuged, and 2,4- dihydroxy-butyric acid is analyzed to nutrient solution by LC-MS/MS.

2,4- dihydroxy butyric acid titre/potency (titer) represents as follows：

Table 2：2,4- dihydroxy butyric acid (DHB) titre for the every kind of bacterial strain evaluated in conical flask.

Symbol (-) refers to that 2, the 4-DHB not as good as 5mg/L is produced, and symbol (+) produces corresponding to 5-15mg/L 2,4-DHB It is raw, and 2, the 4-DHB that symbol (++) corresponds to higher than 15mg/L is produced.

Bacterial strain	DHB titres
		MG1655	-
Bacterial strain 8 to 11	+
		Bacterial strain 17 to 20	++

All recombinant bacterial strains observation 2,4- dihydroxy butyric acid is produced.By collecting thing for NADPH in increase cell to enter Capable genetic modification strengthens yield.

In embodiment 5 under conditions of same as above, on the mixture of xylose and sucrose test strain 23 to 26 (not presented in table 2), and its 2,4-DHB generations are similar to bacterial strain 8 to 11.

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Sequence table

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Gln Val Leu Leu Ala Trp Val Ile Ser His Gln Gly Val Met Ala Ile

225 230 235 240

Pro Lys Ala Ala Thr Ile Ala His Val Gln Gln Asn Ala Ala Val Leu

245 250 255

Glu Val Glu Leu Ser Ser Ala Glu Leu Ala Met Leu Asp Lys Ala Tyr

260 265 270

Pro Ala Pro Lys Gly Lys Thr Ala Leu Asp Met Val

275 280

<210> 7

<211> 298

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 7

Met Val Gln Arg Ile Thr Ile Ala Pro Gln Gly Pro Glu Phe Ser Arg

1 5 10 15

Phe Val Met Gly Tyr Trp Arg Leu Met Asp Trp Asn Met Ser Ala Arg

20 25 30

Gln Leu Val Ser Phe Ile Glu Glu His Leu Asp Leu Gly Val Thr Thr

35 40 45

Val Asp His Ala Asp Ile Tyr Gly Gly Tyr Gln Cys Glu Ala Ala Phe

50 55 60

Gly Glu Ala Leu Lys Leu Ala Pro His Leu Arg Glu Arg Met Glu Ile

65 70 75 80

Val Ser Lys Cys Gly Ile Ala Thr Thr Ala Arg Glu Glu Asn Val Ile

85 90 95

Gly His Tyr Ile Thr Asp Arg Asp His Ile Ile Lys Ser Ala Glu Gln

100 105 110

Ser Leu Ile Asn Leu Ala Thr Asp His Leu Asp Leu Leu Leu Ile His

115 120 125

Arg Pro Asp Pro Leu Met Asp Ala Asp Glu Val Ala Asp Ala Phe Lys

130 135 140

His Leu His Gln Ser Gly Lys Val Arg His Phe Gly Val Ser Asn Phe

145 150 155 160

Thr Pro Ala Gln Phe Ala Leu Leu Gln Ser Arg Leu Pro Phe Thr Leu

165 170 175

Ala Thr Asn Gln Val Glu Ile Ser Pro Val His Gln Pro Leu Leu Leu

180 185 190

Asp Gly Thr Leu Asp Gln Leu Gln Gln Leu Arg Val Arg Pro Met Ala

195 200 205

Trp Ser Cys Leu Gly Gly Gly Arg Leu Phe Asn Asp Asp Tyr Phe Gln

210 215 220

Pro Leu Arg Asp Glu Leu Ala Val Val Ala Glu Glu Leu Asn Ala Gly

225 230 235 240

Ser Ile Glu Gln Val Val Tyr Ala Trp Val Leu Arg Leu Pro Ser Gln

245 250 255

Pro Leu Pro Ile Ile Gly Ser Gly Lys Ile Glu Arg Val Arg Ala Ala

260 265 270

Val Glu Ala Glu Thr Leu Lys Met Thr Arg Gln Gln Trp Phe Arg Ile

275 280 285

Arg Lys Ala Ala Leu Gly Tyr Asp Val Pro

290 295

<210> 8

<211> 122

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 8

Met Trp Leu Leu Asp Gln Trp Ala Glu Arg His Ile Ala Glu Ala Gln

1 5 10 15

Ala Lys Gly Glu Phe Asp Asn Leu Ala Gly Ser Gly Glu Pro Leu Ile

20 25 30

Leu Asp Asp Asp Ser His Val Pro Pro Glu Leu Arg Ala Gly Tyr Arg

35 40 45

Leu Leu Lys Asn Ala Gly Cys Leu Pro Pro Glu Leu Glu Gln Arg Arg

50 55 60

Glu Ala Ile Gln Leu Leu Asp Ile Leu Lys Gly Ile Arg His Asp Asp

65 70 75 80

Pro Gln Tyr Gln Glu Val Ser Arg Arg Leu Ser Leu Leu Glu Leu Lys

85 90 95

Leu Arg Gln Ala Gly Leu Ser Thr Asp Phe Leu Arg Gly Asp Tyr Ala

100 105 110

Asp Lys Leu Leu Asp Lys Ile Asn Asp Asn

115 120

<210> 9

<211> 412

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 9

Met Lys Ala Leu Thr Tyr His Gly Pro His His Val Gln Val Glu Asn

1 5 10 15

Val Pro Asp Pro Gly Val Glu Gln Ala Asp Asp Ile Ile Leu Arg Ile

20 25 30

Thr Ala Thr Ala Ile Cys Gly Ser Asp Leu His Leu Tyr Arg Gly Lys

35 40 45

Ile Pro Gln Val Lys His Gly Asp Ile Phe Gly His Glu Phe Met Gly

50 55 60

Glu Val Val Glu Thr Gly Lys Asp Val Lys Asn Leu Gln Lys Gly Asp

65 70 75 80

Arg Val Val Ile Pro Phe Val Ile Ala Cys Gly Asp Cys Phe Phe Cys

85 90 95

Arg Leu Gln Gln Tyr Ala Ala Cys Glu Asn Thr Asn Ala Gly Lys Gly

100 105 110

Ala Ala Leu Asn Lys Lys Gln Ile Pro Ala Pro Ala Ala Leu Phe Gly

115 120 125

Tyr Ser His Leu Tyr Gly Gly Val Pro Gly Gly Gln Ala Glu Tyr Val

130 135 140

Arg Val Pro Lys Gly Asn Val Gly Pro Phe Lys Val Pro Pro Leu Leu

145 150 155 160

Ser Asp Asp Lys Ala Leu Phe Leu Ser Asp Ile Leu Pro Thr Ala Trp

165 170 175

Gln Ala Ala Lys Asn Ala Gln Ile Gln Gln Gly Ser Ser Val Ala Val

180 185 190

Tyr Gly Ala Gly Pro Val Gly Leu Leu Thr Ile Ala Cys Ala Arg Leu

195 200 205

Leu Gly Ala Glu Gln Ile Phe Val Val Asp His His Pro Tyr Arg Leu

210 215 220

His Phe Ala Ala Asp Arg Tyr Gly Ala Ile Pro Ile Asn Phe Asp Glu

225 230 235 240

Asp Ser Asp Pro Ala Gln Ser Ile Ile Glu Gln Thr Ala Gly His Arg

245 250 255

Gly Val Asp Ala Val Ile Asp Ala Val Gly Phe Glu Ala Lys Gly Ser

260 265 270

Thr Thr Glu Thr Val Leu Thr Asn Leu Lys Leu Glu Gly Ser Ser Gly

275 280 285

Lys Ala Leu Arg Gln Cys Ile Ala Ala Val Arg Arg Gly Gly Ile Val

290 295 300

Ser Val Pro Gly Val Tyr Ala Gly Phe Ile His Gly Phe Leu Phe Gly

305 310 315 320

Asp Ala Phe Asp Lys Gly Leu Ser Phe Lys Met Gly Gln Thr His Val

325 330 335

His Ala Trp Leu Gly Glu Leu Leu Pro Leu Ile Glu Lys Gly Leu Leu

340 345 350

Lys Pro Glu Glu Ile Val Thr His Tyr Met Pro Phe Glu Glu Ala Ala

355 360 365

Arg Gly Tyr Glu Ile Phe Glu Lys Arg Glu Glu Glu Cys Arg Lys Val

370 375 380

Ile Leu Val Pro Gly Ala Gln Ser Ala Glu Ala Ala Gln Lys Ala Val

385 390 395 400

Ser Gly Leu Val Asn Ala Met Pro Gly Gly Thr Ile

405 410

<210> 10

<211> 362

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 10

Met Pro His Asn Pro Ile Arg Val Val Val Gly Pro Ala Asn Tyr Phe

1 5 10 15

Ser His Pro Gly Ser Phe Asn His Leu His Asp Phe Phe Thr Asp Glu

20 25 30

Gln Leu Ser Arg Ala Val Trp Ile Tyr Gly Lys Arg Ala Ile Ala Ala

35 40 45

Ala Gln Thr Lys Leu Pro Pro Ala Phe Gly Leu Pro Gly Ala Lys His

50 55 60

Ile Leu Phe Arg Gly His Cys Ser Glu Ser Asp Val Gln Gln Leu Ala

65 70 75 80

Ala Glu Ser Gly Asp Asp Arg Ser Val Val Ile Gly Val Gly Gly Gly

85 90 95

Ala Leu Leu Asp Thr Ala Lys Ala Leu Ala Arg Arg Leu Gly Leu Pro

100 105 110

Phe Val Ala Val Pro Thr Ile Ala Ala Thr Cys Ala Ala Trp Thr Pro

115 120 125

Leu Ser Val Trp Tyr Asn Asp Ala Gly Gln Ala Leu His Tyr Glu Ile

130 135 140

Phe Asp Asp Ala Asn Phe Met Val Leu Val Glu Pro Glu Ile Ile Leu

145 150 155 160

Asn Ala Pro Gln Gln Tyr Leu Leu Ala Gly Ile Gly Asp Thr Leu Ala

165 170 175

Lys Trp Tyr Glu Ala Val Val Leu Ala Pro Gln Pro Glu Thr Leu Pro

180 185 190

Leu Thr Val Arg Leu Gly Ile Asn Asn Ala Gln Ala Ile Arg Asp Val

195 200 205

Leu Leu Asn Ser Ser Glu Gln Ala Leu Ser Asp Gln Gln Asn Gln Gln

210 215 220

Leu Thr Gln Ser Phe Cys Asp Val Val Asp Ala Ile Ile Ala Gly Gly

225 230 235 240

Gly Met Val Gly Gly Leu Gly Asp Arg Phe Thr Arg Val Ala Ala Ala

245 250 255

His Ala Val His Asn Gly Leu Thr Val Leu Pro Gln Thr Glu Lys Phe

260 265 270

Leu His Gly Thr Lys Val Ala Tyr Gly Ile Leu Val Gln Ser Ala Leu

275 280 285

Leu Gly Gln Asp Asp Val Leu Ala Gln Leu Thr Gly Ala Tyr Gln Arg

290 295 300

Phe His Leu Pro Thr Thr Leu Ala Glu Leu Glu Val Asp Ile Asn Asn

305 310 315 320

Gln Ala Glu Ile Asp Lys Val Ile Ala His Thr Leu Arg Pro Val Glu

325 330 335

Ser Ile His Tyr Leu Pro Val Thr Leu Thr Pro Asp Thr Leu Arg Ala

340 345 350

Ala Phe Lys Lys Val Glu Ser Phe Lys Ala

355 360

<210> 11

<211> 347

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 11

Met Lys Asn Ser Lys Ala Ile Leu Gln Val Pro Gly Thr Met Lys Ile

1 5 10 15

Ile Ser Ala Glu Ile Pro Val Pro Lys Glu Asp Glu Val Leu Ile Lys

20 25 30

Val Glu Tyr Val Gly Ile Cys Gly Ser Asp Val His Gly Phe Glu Ser

35 40 45

Gly Pro Phe Ile Pro Pro Lys Asp Pro Asn Gln Glu Ile Gly Leu Gly

50 55 60

His Glu Cys Ala Gly Thr Val Val Ala Val Gly Ser Arg Val Arg Lys

65 70 75 80

Phe Lys Pro Gly Asp Arg Val Asn Ile Glu Pro Gly Val Pro Cys Gly

85 90 95

His Cys Arg Tyr Cys Leu Glu Gly Lys Tyr Asn Ile Cys Pro Asp Val

100 105 110

Asp Phe Met Ala Thr Gln Pro Asn Tyr Arg Gly Ala Leu Thr His Tyr

115 120 125

Leu Cys His Pro Glu Ser Phe Thr Tyr Lys Leu Pro Asp Asn Met Asp

130 135 140

Thr Met Glu Gly Ala Leu Val Glu Pro Ala Ala Val Gly Met His Ala

145 150 155 160

Ala Met Leu Ala Asp Val Lys Pro Gly Lys Lys Ile Ile Ile Leu Gly

165 170 175

Ala Gly Cys Ile Gly Leu Met Thr Leu Gln Ala Cys Lys Cys Leu Gly

180 185 190

Ala Thr Glu Ile Ala Val Val Asp Val Leu Glu Lys Arg Leu Ala Met

195 200 205

Ala Glu Gln Leu Gly Ala Thr Val Val Ile Asn Gly Ala Lys Glu Asp

210 215 220

Thr Ile Ala Arg Cys Gln Gln Phe Thr Glu Asp Met Gly Ala Asp Ile

225 230 235 240

Val Phe Glu Thr Ala Gly Ser Ala Val Thr Val Lys Gln Ala Pro Tyr

245 250 255

Leu Val Met Arg Gly Gly Lys Ile Met Ile Val Gly Thr Val Pro Gly

260 265 270

Asp Ser Ala Ile Asn Phe Leu Lys Ile Asn Arg Glu Val Thr Ile Gln

275 280 285

Thr Val Phe Arg Tyr Ala Asn Arg Tyr Pro Val Thr Ile Glu Ala Ile

290 295 300

Ser Ser Gly Arg Phe Asp Val Lys Ser Met Val Thr His Ile Tyr Asp

305 310 315 320

Tyr Arg Asp Val Gln Gln Ala Phe Glu Glu Ser Val Asn Asn Lys Arg

325 330 335

Asp Ile Ile Lys Gly Val Ile Lys Ile Ser Asp

340 345

<210> 12

<211> 358

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 12

Met Lys Ala Leu Ala Arg Phe Gly Lys Ala Phe Gly Gly Tyr Lys Met

1 5 10 15

Ile Asp Val Pro Gln Pro Met Cys Gly Pro Glu Asp Val Val Ile Glu

20 25 30

Ile Lys Ala Ala Ala Ile Cys Gly Ala Asp Met Lys His Tyr Asn Val

35 40 45

Asp Ser Gly Ser Asp Glu Phe Asn Ser Ile Arg Gly His Glu Phe Ala

50 55 60

Gly Cys Ile Ala Gln Val Gly Glu Lys Val Lys Asp Trp Lys Val Gly

65 70 75 80

Gln Arg Val Val Ser Asp Asn Ser Gly His Val Cys Gly Val Cys Pro

85 90 95

Ala Cys Glu Gln Gly Asp Phe Leu Cys Cys Thr Glu Lys Val Asn Leu

100 105 110

Gly Leu Asp Asn Asn Thr Trp Gly Gly Gly Phe Ser Lys Tyr Cys Leu

115 120 125

Val Pro Gly Glu Ile Leu Lys Ile His Arg His Ala Leu Trp Glu Ile

130 135 140

Pro Asp Gly Val Asp Tyr Glu Asp Ala Ala Val Leu Asp Pro Ile Cys

145 150 155 160

Asn Ala Tyr Lys Ser Ile Ala Gln Gln Ser Lys Phe Leu Pro Gly Gln

165 170 175

Asp Val Val Val Ile Gly Thr Gly Pro Leu Gly Leu Phe Ser Val Gln

180 185 190

Met Ala Arg Ile Met Gly Ala Val Asn Ile Val Val Val Gly Leu Gln

195 200 205

Glu Asp Val Ala Val Arg Phe Pro Val Ala Lys Glu Leu Gly Ala Thr

210 215 220

Ala Val Val Asn Gly Ser Thr Glu Asp Val Val Ala Arg Cys Gln Gln

225 230 235 240

Ile Cys Gly Lys Asp Asn Leu Gly Leu Val Ile Glu Cys Ser Gly Ala

245 250 255

Asn Ile Ala Leu Lys Gln Ala Ile Glu Met Leu Arg Pro Asn Gly Glu

260 265 270

Val Val Arg Val Gly Met Gly Phe Lys Pro Leu Asp Phe Ser Ile Asn

275 280 285

Asp Ile Thr Ala Trp Asn Lys Ser Ile Ile Gly His Met Ala Tyr Asp

290 295 300

Ser Thr Ser Trp Arg Asn Ala Ile Arg Leu Leu Ala Ser Gly Ala Ile

305 310 315 320

Lys Val Lys Pro Met Ile Thr His Arg Ile Gly Leu Ser Gln Trp Arg

325 330 335

Glu Gly Phe Asp Ala Met Val Asp Lys Thr Ala Ile Lys Val Ile Met

340 345 350

Thr Tyr Asp Phe Asp Glu

355

<210> 13

<211> 390

<212> PRT

<213>Clostridium acetobutylicum (Clostridium acetobutylicum)

<400> 13

Met Val Asp Phe Glu Tyr Ser Ile Pro Thr Arg Ile Phe Phe Gly Lys

1 5 10 15

Asp Lys Ile Asn Val Leu Gly Arg Glu Leu Lys Lys Tyr Gly Ser Lys

20 25 30

Val Leu Ile Val Tyr Gly Gly Gly Ser Ile Lys Arg Asn Gly Ile Tyr

35 40 45

Asp Lys Ala Val Ser Ile Leu Glu Lys Asn Ser Ile Lys Phe Tyr Glu

50 55 60

Leu Ala Gly Val Glu Pro Asn Pro Arg Val Thr Thr Val Glu Lys Gly

65 70 75 80

Val Lys Ile Cys Arg Glu Asn Gly Val Glu Val Val Leu Ala Ile Gly

85 90 95

Gly Gly Ser Ala Ile Asp Cys Ala Lys Val Ile Ala Ala Ala Cys Glu

100 105 110

Tyr Asp Gly Asn Pro Trp Asp Ile Val Leu Asp Gly Ser Lys Ile Lys

115 120 125

Arg Val Leu Pro Ile Ala Ser Ile Leu Thr Ile Ala Ala Thr Gly Ser

130 135 140

Glu Met Asp Thr Trp Ala Val Ile Asn Asn Met Asp Thr Asn Glu Lys

145 150 155 160

Leu Ile Ala Ala His Pro Asp Met Ala Pro Lys Phe Ser Ile Leu Asp

165 170 175

Pro Thr Tyr Thr Tyr Thr Val Pro Thr Asn Gln Thr Ala Ala Gly Thr

180 185 190

Ala Asp Ile Met Ser His Ile Phe Glu Val Tyr Phe Ser Asn Thr Lys

195 200 205

Thr Ala Tyr Leu Gln Asp Arg Met Ala Glu Ala Leu Leu Arg Thr Cys

210 215 220

Ile Lys Tyr Gly Gly Ile Ala Leu Glu Lys Pro Asp Asp Tyr Glu Ala

225 230 235 240

Arg Ala Asn Leu Met Trp Ala Ser Ser Leu Ala Ile Asn Gly Leu Leu

245 250 255

Thr Tyr Gly Lys Asp Thr Asn Trp Ser Val His Leu Met Glu His Glu

260 265 270

Leu Ser Ala Tyr Tyr Asp Ile Thr His Gly Val Gly Leu Ala Ile Leu

275 280 285

Thr Pro Asn Trp Met Glu Tyr Ile Leu Asn Asn Asp Thr Val Tyr Lys

290 295 300

Phe Val Glu Tyr Gly Val Asn Val Trp Gly Ile Asp Lys Glu Lys Asn

305 310 315 320

His Tyr Asp Ile Ala His Gln Ala Ile Gln Lys Thr Arg Asp Tyr Phe

325 330 335

Val Asn Val Leu Gly Leu Pro Ser Arg Leu Arg Asp Val Gly Ile Glu

340 345 350

Glu Glu Lys Leu Asp Ile Met Ala Lys Glu Ser Val Lys Leu Thr Gly

355 360 365

Gly Thr Ile Gly Asn Leu Arg Pro Val Asn Ala Ser Glu Val Leu Gln

370 375 380

Ile Phe Lys Lys Ser Val

385 390

<210> 14

<211> 389

<212> PRT

<213>Clostridium acetobutylicum (Clostridium acetobutylicum)

<400> 14

Met Leu Ser Phe Asp Tyr Ser Ile Pro Thr Lys Val Phe Phe Gly Lys

1 5 10 15

Gly Lys Ile Asp Val Ile Gly Glu Glu Ile Lys Lys Tyr Gly Ser Arg

20 25 30

Val Leu Ile Val Tyr Gly Gly Gly Ser Ile Lys Arg Asn Gly Ile Tyr

35 40 45

Asp Arg Ala Thr Ala Ile Leu Lys Glu Asn Asn Ile Ala Phe Tyr Glu

50 55 60

Leu Ser Gly Val Glu Pro Asn Pro Arg Ile Thr Thr Val Lys Lys Gly

65 70 75 80

Ile Glu Ile Cys Arg Glu Asn Asn Val Asp Leu Val Leu Ala Ile Gly

85 90 95

Gly Gly Ser Ala Ile Asp Cys Ser Lys Val Ile Ala Ala Gly Val Tyr

100 105 110

Tyr Asp Gly Asp Thr Trp Asp Met Val Lys Asp Pro Ser Lys Ile Thr

115 120 125

Lys Val Leu Pro Ile Ala Ser Ile Leu Thr Leu Ser Ala Thr Gly Ser

130 135 140

Glu Met Asp Gln Ile Ala Val Ile Ser Asn Met Glu Thr Asn Glu Lys

145 150 155 160

Leu Gly Val Gly His Asp Asp Met Arg Pro Lys Phe Ser Val Leu Asp

165 170 175

Pro Thr Tyr Thr Phe Thr Val Pro Lys Asn Gln Thr Ala Ala Gly Thr

180 185 190

Ala Asp Ile Met Ser His Thr Phe Glu Ser Tyr Phe Ser Gly Val Glu

195 200 205

Gly Ala Tyr Val Gln Asp Gly Ile Ala Glu Ala Ile Leu Arg Thr Cys

210 215 220

Ile Lys Tyr Gly Lys Ile Ala Met Glu Lys Thr Asp Asp Tyr Glu Ala

225 230 235 240

Arg Ala Asn Leu Met Trp Ala Ser Ser Leu Ala Ile Asn Gly Leu Leu

245 250 255

Ser Leu Gly Lys Asp Arg Lys Trp Ser Cys His Pro Met Glu His Glu

260 265 270

Leu Ser Ala Tyr Tyr Asp Ile Thr His Gly Val Gly Leu Ala Ile Leu

275 280 285

Thr Pro Asn Trp Met Glu Tyr Ile Leu Asn Asp Asp Thr Leu His Lys

290 295 300

Phe Val Ser Tyr Gly Ile Asn Val Trp Gly Ile Asp Lys Asn Lys Asp

305 310 315 320

Asn Tyr Glu Ile Ala Arg Glu Ala Ile Lys Asn Thr Arg Glu Tyr Phe

325 330 335

Asn Ser Leu Gly Ile Pro Ser Lys Leu Arg Glu Val Gly Ile Gly Lys

340 345 350

Asp Lys Leu Glu Leu Met Ala Lys Gln Ala Val Arg Asn Ser Gly Gly

355 360 365

Thr Ile Gly Ser Leu Arg Pro Ile Asn Ala Glu Asp Val Leu Glu Ile

370 375 380

Phe Lys Lys Ser Tyr

385

<210> 15

<211> 386

<212> PRT

<213>Clostridium acetobutylicum (Clostridium acetobutylicum)

<400> 15

Met Tyr Asn Phe Asp Phe Phe Asn Pro Thr His Ile Val Phe Gly Lys

1 5 10 15

Asp Arg Leu Asn Glu Leu Asp Asn Leu Val Pro Arg Asp Ala Lys Val

20 25 30

Leu Val Leu Tyr Gly Gly Gly Ser Val Lys Lys Phe Gly Thr Leu Glu

35 40 45

Lys Val Ile Asn Gly Leu Gly Asn Arg Gln Val Ile Glu Phe Gly Gly

50 55 60

Ile Glu Pro Asn Pro Gln Phe Thr Thr Leu Met Lys Ala Val Asp Ile

65 70 75 80

Val Lys Lys Glu Asn Ile Asp Phe Leu Leu Ala Val Gly Gly Gly Ser

85 90 95

Val Met Asp Gly Thr Lys Phe Val Ala Leu Ala Ala Tyr Tyr Glu Gly

100 105 110

Asp Asn Ala Ala Glu Ile Leu Tyr Ser Arg Glu Lys Ala Ala Ala Ile

115 120 125

Asn Lys Ala Val Pro Leu Gly Thr Val Val Thr Leu Pro Ala Thr Gly

130 135 140

Ser Glu Met Asn Asn Gly Gly Val Ile Ser Tyr Glu His Gly Lys Tyr

145 150 155 160

Gly Phe Gly Ser Lys Leu Val Phe Pro Lys Phe Ser Val Leu Asp Pro

165 170 175

Thr Leu Thr Tyr Thr Leu Pro Glu Ser Gln Val Ala Asn Gly Val Ala

180 185 190

Asp Thr Phe Val His Val Leu Glu Gln Tyr Ala Thr Phe Lys Ala Glu

195 200 205

Gly Arg Phe Gln Asp Arg Thr Ala Glu Gly Ile Leu Gln Thr Leu Ile

210 215 220

Glu Ile Gly Arg Lys Thr Ile Asp Asn Pro Thr Asp Tyr Asp Thr Arg

225 230 235 240

Ala Asn Leu Val Trp Cys Ala Thr Met Ala Leu Asn Gly Leu Ile Gly

245 250 255

Ala Gly Val Pro Gln Asp Trp Ser Thr His Met Ile Gly His Glu Leu

260 265 270

Thr Ala Met Phe Gly Ile Asp His Gly Lys Thr Leu Ala Ile Ile Leu

275 280 285

Pro Ser Ile Trp Asn Val Met Arg Glu Gln Lys Lys Gly Lys Ile Leu

290 295 300

Gln Tyr Ala Glu Arg Val Leu Gly Ile Thr Glu Gly Asp Asp Asp Ser

305 310 315 320

Arg Ile Asp Leu Ala Ile Leu Arg Thr Arg Glu Phe Phe Glu Ser Leu

325 330 335

Gly Ile Lys Thr His Leu Ser Glu Tyr Gly Val Thr Ala Asp Lys Ile

340 345 350

Asp Asp Ile Val Asn Ala Leu Asp Lys His Gly Met Lys Ala Leu Ser

355 360 365

Glu Thr Gly Ala Ile Thr Leu Glu Val Ser Arg Lys Ile Leu Glu Gly

370 375 380

Ala Met

385

<210> 16

<211> 348

<212> PRT

<213>Saccharomyces cerevisiae (Saccharomyces cerevisiae)

<400> 16

Met Ser Ile Pro Glu Thr Gln Lys Gly Val Ile Phe Tyr Glu Ser His

1 5 10 15

Gly Lys Leu Glu Tyr Lys Asp Ile Pro Val Pro Lys Pro Lys Ala Asn

20 25 30

Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu

35 40 45

His Ala Trp His Gly Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val

50 55 60

Gly Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val

65 70 75 80

Lys Gly Trp Lys Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly

85 90 95

Ser Cys Met Ala Cys Glu Tyr Cys Glu Leu Gly Asn Glu Ser Asn Cys

100 105 110

Pro His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln

115 120 125

Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro Gln Gly Thr

130 135 140

Asp Leu Ala Gln Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr

145 150 155 160

Lys Ala Leu Lys Ser Ala Asn Leu Met Ala Gly His Trp Val Ala Ile

165 170 175

Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys

180 185 190

Ala Met Gly Tyr Arg Val Leu Gly Ile Asp Gly Gly Glu Gly Lys Glu

195 200 205

Glu Leu Phe Arg Ser Ile Gly Gly Glu Val Phe Ile Asp Phe Thr Lys

210 215 220

Glu Lys Asp Ile Val Gly Ala Val Leu Lys Ala Thr Asp Gly Gly Ala

225 230 235 240

His Gly Val Ile Asn Val Ser Val Ser Glu Ala Ala Ile Glu Ala Ser

245 250 255

Thr Arg Tyr Val Arg Ala Asn Gly Thr Thr Val Leu Val Gly Met Pro

260 265 270

Ala Gly Ala Lys Cys Cys Ser Asp Val Phe Asn Gln Val Val Lys Ser

275 280 285

Ile Ser Ile Val Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu

290 295 300

Ala Leu Asp Phe Phe Ala Arg Gly Leu Val Lys Ser Pro Ile Lys Val

305 310 315 320

Val Gly Leu Ser Thr Leu Pro Glu Ile Tyr Glu Lys Met Glu Lys Gly

325 330 335

Gln Ile Val Gly Arg Tyr Val Val Asp Thr Ser Lys

340 345

<210> 17

<211> 348

<212> PRT

<213>Saccharomyces cerevisiae (Saccharomyces cerevisiae)

<400> 17

Met Ser Ile Pro Glu Thr Gln Lys Ala Ile Ile Phe Tyr Glu Ser Asn

1 5 10 15

Gly Lys Leu Glu His Lys Asp Ile Pro Val Pro Lys Pro Lys Pro Asn

20 25 30

Glu Leu Leu Ile Asn Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu

35 40 45

His Ala Trp His Gly Asp Trp Pro Leu Pro Thr Lys Leu Pro Leu Val

50 55 60

Gly Gly His Glu Gly Ala Gly Val Val Val Gly Met Gly Glu Asn Val

65 70 75 80

Lys Gly Trp Lys Ile Gly Asp Tyr Ala Gly Ile Lys Trp Leu Asn Gly

85 90 95

Ser Cys Met Ala Cys Glu Tyr Cys Glu Leu Gly Asn Glu Ser Asn Cys

100 105 110

Pro His Ala Asp Leu Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Glu

115 120 125

Tyr Ala Thr Ala Asp Ala Val Gln Ala Ala His Ile Pro Gln Gly Thr

130 135 140

Asp Leu Ala Glu Val Ala Pro Ile Leu Cys Ala Gly Ile Thr Val Tyr

145 150 155 160

Lys Ala Leu Lys Ser Ala Asn Leu Arg Ala Gly His Trp Ala Ala Ile

165 170 175

Ser Gly Ala Ala Gly Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Lys

180 185 190

Ala Met Gly Tyr Arg Val Leu Gly Ile Asp Gly Gly Pro Gly Lys Glu

195 200 205

Glu Leu Phe Thr Ser Leu Gly Gly Glu Val Phe Ile Asp Phe Thr Lys

210 215 220

Glu Lys Asp Ile Val Ser Ala Val Val Lys Ala Thr Asn Gly Gly Ala

225 230 235 240

His Gly Ile Ile Asn Val Ser Val Ser Glu Ala Ala Ile Glu Ala Ser

245 250 255

Thr Arg Tyr Cys Arg Ala Asn Gly Thr Val Val Leu Val Gly Leu Pro

260 265 270

Ala Gly Ala Lys Cys Ser Ser Asp Val Phe Asn His Val Val Lys Ser

275 280 285

Ile Ser Ile Val Gly Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu

290 295 300

Ala Leu Asp Phe Phe Ala Arg Gly Leu Val Lys Ser Pro Ile Lys Val

305 310 315 320

Val Gly Leu Ser Ser Leu Pro Glu Ile Tyr Glu Lys Met Glu Lys Gly

325 330 335

Gln Ile Ala Gly Arg Tyr Val Val Asp Thr Ser Lys

340 345

<210> 18

<211> 375

<212> PRT

<213>Saccharomyces cerevisiae (Saccharomyces cerevisiae)

<400> 18

Met Leu Arg Thr Ser Thr Leu Phe Thr Arg Arg Val Gln Pro Ser Leu

1 5 10 15

Phe Ser Arg Asn Ile Leu Arg Leu Gln Ser Thr Ala Ala Ile Pro Lys

20 25 30

Thr Gln Lys Gly Val Ile Phe Tyr Glu Asn Lys Gly Lys Leu His Tyr

35 40 45

Lys Asp Ile Pro Val Pro Glu Pro Lys Pro Asn Glu Ile Leu Ile Asn

50 55 60

Val Lys Tyr Ser Gly Val Cys His Thr Asp Leu His Ala Trp His Gly

65 70 75 80

Asp Trp Pro Leu Pro Val Lys Leu Pro Leu Val Gly Gly His Glu Gly

85 90 95

Ala Gly Val Val Val Lys Leu Gly Ser Asn Val Lys Gly Trp Lys Val

100 105 110

Gly Asp Leu Ala Gly Ile Lys Trp Leu Asn Gly Ser Cys Met Thr Cys

115 120 125

Glu Phe Cys Glu Ser Gly His Glu Ser Asn Cys Pro Asp Ala Asp Leu

130 135 140

Ser Gly Tyr Thr His Asp Gly Ser Phe Gln Gln Phe Ala Thr Ala Asp

145 150 155 160

Ala Ile Gln Ala Ala Lys Ile Gln Gln Gly Thr Asp Leu Ala Glu Val

165 170 175

Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys Ala Leu Lys Glu

180 185 190

Ala Asp Leu Lys Ala Gly Asp Trp Val Ala Ile Ser Gly Ala Ala Gly

195 200 205

Gly Leu Gly Ser Leu Ala Val Gln Tyr Ala Thr Ala Met Gly Tyr Arg

210 215 220

Val Leu Gly Ile Asp Ala Gly Glu Glu Lys Glu Lys Leu Phe Lys Lys

225 230 235 240

Leu Gly Gly Glu Val Phe Ile Asp Phe Thr Lys Thr Lys Asn Met Val

245 250 255

Ser Asp Ile Gln Glu Ala Thr Lys Gly Gly Pro His Gly Val Ile Asn

260 265 270

Val Ser Val Ser Glu Ala Ala Ile Ser Leu Ser Thr Glu Tyr Val Arg

275 280 285

Pro Cys Gly Thr Val Val Leu Val Gly Leu Pro Ala Asn Ala Tyr Val

290 295 300

Lys Ser Glu Val Phe Ser His Val Val Lys Ser Ile Asn Ile Lys Gly

305 310 315 320

Ser Tyr Val Gly Asn Arg Ala Asp Thr Arg Glu Ala Leu Asp Phe Phe

325 330 335

Ser Arg Gly Leu Ile Lys Ser Pro Ile Lys Ile Val Gly Leu Ser Glu

340 345 350

Leu Pro Lys Val Tyr Asp Leu Met Glu Lys Gly Lys Ile Leu Gly Arg

355 360 365

Tyr Val Val Asp Thr Ser Lys

370 375

<210> 19

<211> 382

<212> PRT

<213>Saccharomyces cerevisiae (Saccharomyces cerevisiae)

<400> 19

Met Ser Ser Val Thr Gly Phe Tyr Ile Pro Pro Ile Ser Phe Phe Gly

1 5 10 15

Glu Gly Ala Leu Glu Glu Thr Ala Asp Tyr Ile Lys Asn Lys Asp Tyr

20 25 30

Lys Lys Ala Leu Ile Val Thr Asp Pro Gly Ile Ala Ala Ile Gly Leu

35 40 45

Ser Gly Arg Val Gln Lys Met Leu Glu Glu Arg Asp Leu Asn Val Ala

50 55 60

Ile Tyr Asp Lys Thr Gln Pro Asn Pro Asn Ile Ala Asn Val Thr Ala

65 70 75 80

Gly Leu Lys Val Leu Lys Glu Gln Asn Ser Glu Ile Val Val Ser Ile

85 90 95

Gly Gly Gly Ser Ala His Asp Asn Ala Lys Ala Ile Ala Leu Leu Ala

100 105 110

Thr Asn Gly Gly Glu Ile Gly Asp Tyr Glu Gly Val Asn Gln Ser Lys

115 120 125

Lys Ala Ala Leu Pro Leu Phe Ala Ile Asn Thr Thr Ala Gly Thr Ala

130 135 140

Ser Glu Met Thr Arg Phe Thr Ile Ile Ser Asn Glu Glu Lys Lys Ile

145 150 155 160

Lys Met Ala Ile Ile Asp Asn Asn Val Thr Pro Ala Val Ala Val Asn

165 170 175

Asp Pro Ser Thr Met Phe Gly Leu Pro Pro Ala Leu Thr Ala Ala Thr

180 185 190

Gly Leu Asp Ala Leu Thr His Cys Ile Glu Ala Tyr Val Ser Thr Ala

195 200 205

Ser Asn Pro Ile Thr Asp Ala Cys Ala Leu Lys Gly Ile Asp Leu Ile

210 215 220

Asn Glu Ser Leu Val Ala Ala Tyr Lys Asp Gly Lys Asp Lys Lys Ala

225 230 235 240

Arg Thr Asp Met Cys Tyr Ala Glu Tyr Leu Ala Gly Met Ala Phe Asn

245 250 255

Asn Ala Ser Leu Gly Tyr Val His Ala Leu Ala His Gln Leu Gly Gly

260 265 270

Phe Tyr His Leu Pro His Gly Val Cys Asn Ala Val Leu Leu Pro His

275 280 285

Val Gln Glu Ala Asn Met Gln Cys Pro Lys Ala Lys Lys Arg Leu Gly

290 295 300

Glu Ile Ala Leu His Phe Gly Ala Ser Gln Glu Asp Pro Glu Glu Thr

305 310 315 320

Ile Lys Ala Leu His Val Leu Asn Arg Thr Met Asn Ile Pro Arg Asn

325 330 335

Leu Lys Glu Leu Gly Val Lys Thr Glu Asp Phe Glu Ile Leu Ala Glu

340 345 350

His Ala Met His Asp Ala Cys His Leu Thr Asn Pro Val Gln Phe Thr

355 360 365

Lys Glu Gln Val Val Ala Ile Ile Lys Lys Ala Tyr Glu Tyr

370 375 380

<210> 20

<211> 331

<212> PRT

<213>Bacillus subtilis (Bacillus subtilis)

<400> 20

Met Glu Tyr Thr Ser Ile Ala Asp Thr Gly Ile Glu Ala Ser Arg Ile

1 5 10 15

Gly Leu Gly Thr Trp Ala Ile Gly Gly Thr Met Trp Gly Gly Thr Asp

20 25 30

Glu Lys Thr Ser Ile Glu Thr Ile Arg Ala Ala Leu Asp Gln Gly Ile

35 40 45

Thr Leu Ile Asp Thr Ala Pro Ala Tyr Gly Phe Gly Gln Ser Glu Glu

50 55 60

Ile Val Gly Lys Ala Ile Lys Glu Tyr Gly Lys Arg Asp Gln Val Ile

65 70 75 80

Leu Ala Thr Lys Thr Ala Leu Asp Trp Lys Asn Asn Gln Leu Phe Arg

85 90 95

His Ala Asn Arg Ala Arg Ile Val Glu Glu Val Glu Asn Ser Leu Lys

100 105 110

Arg Leu Gln Thr Asp Tyr Ile Asp Leu Tyr Gln Val His Trp Pro Asp

115 120 125

Pro Leu Val Pro Ile Glu Glu Thr Ala Glu Val Met Lys Glu Leu Tyr

130 135 140

Asp Ala Gly Lys Ile Arg Ala Ile Gly Val Ser Asn Phe Ser Ile Glu

145 150 155 160

Gln Met Asp Thr Phe Arg Ala Val Ala Pro Leu His Thr Ile Gln Pro

165 170 175

Pro Tyr Asn Leu Phe Glu Arg Glu Met Glu Glu Ser Val Leu Pro Tyr

180 185 190

Ala Lys Asp Asn Lys Ile Thr Thr Leu Leu Tyr Gly Ser Leu Cys Arg

195 200 205

Gly Leu Leu Thr Gly Lys Met Thr Glu Glu Tyr Thr Phe Glu Gly Asp

210 215 220

Asp Leu Arg Asn His Asp Pro Lys Phe Gln Lys Pro Arg Phe Lys Glu

225 230 235 240

Tyr Leu Ser Ala Val Asn Gln Leu Asp Lys Leu Ala Lys Thr Arg Tyr

245 250 255

Gly Lys Ser Val Ile His Leu Ala Val Arg Trp Ile Leu Asp Gln Pro

260 265 270

Gly Ala Asp Ile Ala Leu Trp Gly Ala Arg Lys Pro Gly Gln Leu Glu

275 280 285

Ala Leu Ser Glu Ile Thr Gly Trp Thr Leu Asn Ser Glu Asp Gln Lys

290 295 300

Asp Ile Asn Thr Ile Leu Glu Asn Thr Ile Ser Asp Pro Val Gly Pro

305 310 315 320

Glu Phe Met Ala Pro Pro Thr Arg Glu Glu Ile

325 330

<210> 21

<211> 332

<212> PRT

<213>Gluconobacter oxydans (Gluconobacter oxydans)

<400> 21

Met Ala Ser Asp Thr Ile Arg Ile Pro Gly Ile Asp Thr Pro Leu Ser

1 5 10 15

Arg Val Ala Leu Gly Thr Trp Ala Ile Gly Gly Trp Met Trp Gly Gly

20 25 30

Pro Asp Asp Asp Asn Gly Val Arg Thr Ile His Ala Ala Leu Asp Glu

35 40 45

Gly Ile Asn Leu Ile Asp Thr Ala Pro Val Tyr Gly Phe Gly His Ser

50 55 60

Glu Glu Ile Val Gly Arg Ala Leu Ala Glu Lys Pro Asn Lys Ala His

65 70 75 80

Val Ala Thr Lys Leu Gly Leu His Trp Val Gly Glu Asp Glu Lys Asn

85 90 95

Met Lys Val Phe Arg Asp Ser Arg Pro Ala Arg Ile Arg Lys Glu Val

100 105 110

Glu Asp Ser Leu Arg Arg Leu Arg Val Glu Thr Ile Asp Leu Glu Gln

115 120 125

Ile His Trp Pro Asp Asp Lys Thr Pro Ile Asp Glu Ser Ala Arg Glu

130 135 140

Leu Gln Lys Leu His Gln Asp Gly Lys Ile Arg Ala Leu Gly Val Ser

145 150 155 160

Asn Phe Ser Pro Glu Gln Met Asp Ile Phe Arg Glu Val Ala Pro Leu

165 170 175

Ala Thr Ile Gln Pro Pro Leu Asn Leu Phe Glu Arg Thr Ile Glu Lys

180 185 190

Asp Ile Leu Pro Tyr Ala Glu Lys His Asn Ala Val Val Leu Ala Tyr

195 200 205

Gly Ala Leu Cys Arg Gly Leu Leu Thr Gly Lys Met Asn Arg Asp Thr

210 215 220

Thr Phe Pro Lys Asp Asp Leu Arg Ser Asn Asp Pro Lys Phe Gln Lys

225 230 235 240

Pro Asn Phe Glu Lys Tyr Leu Ala Ala Met Asp Glu Phe Glu Lys Leu

245 250 255

Ala Glu Lys Arg Gly Lys Ser Val Met Ala Phe Ala Val Arg Trp Val

260 265 270

Leu Asp Gln Gly Pro Val Ile Ala Leu Trp Gly Ala Arg Lys Pro Gly

275 280 285

Gln Val Ser Gly Val Lys Asp Val Phe Gly Trp Ser Leu Thr Asp Glu

290 295 300

Glu Lys Lys Ala Val Asp Asp Ile Leu Ala Arg His Val Pro Asn Pro

305 310 315 320

Ile Asp Pro Thr Phe Met Ala Pro Pro Ala Arg Asp

325 330

<210> 22

<211> 339

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 22

Met Ser Met Ile Lys Ser Tyr Ala Ala Lys Glu Ala Gly Gly Glu Leu

1 5 10 15

Glu Val Tyr Glu Tyr Asp Pro Gly Glu Leu Arg Pro Gln Asp Val Glu

20 25 30

Val Gln Val Asp Tyr Cys Gly Ile Cys His Ser Asp Leu Ser Met Ile

35 40 45

Asp Asn Glu Trp Gly Phe Ser Gln Tyr Pro Leu Val Ala Gly His Glu

50 55 60

Val Ile Gly Arg Val Val Ala Leu Gly Ser Ala Ala Gln Asp Lys Gly

65 70 75 80

Leu Gln Val Gly Gln Arg Val Gly Ile Gly Trp Thr Ala Arg Ser Cys

85 90 95

Gly His Cys Asp Ala Cys Ile Ser Gly Asn Gln Ile Asn Cys Glu Gln

100 105 110

Gly Ala Val Pro Thr Ile Met Asn Arg Gly Gly Phe Ala Glu Lys Leu

115 120 125

Arg Ala Asp Trp Gln Trp Val Ile Pro Leu Pro Glu Asn Ile Asp Ile

130 135 140

Glu Ser Ala Gly Pro Leu Leu Cys Gly Gly Ile Thr Val Phe Lys Pro

145 150 155 160

Leu Leu Met His His Ile Thr Ala Thr Ser Arg Val Gly Val Ile Gly

165 170 175

Ile Gly Gly Leu Gly His Ile Ala Ile Lys Leu Leu His Ala Met Gly

180 185 190

Cys Glu Val Thr Ala Phe Ser Ser Asn Pro Ala Lys Glu Gln Glu Val

195 200 205

Leu Ala Met Gly Ala Asp Lys Val Val Asn Ser Arg Asp Pro Gln Ala

210 215 220

Leu Lys Ala Leu Ala Gly Gln Phe Asp Leu Ile Ile Asn Thr Val Asn

225 230 235 240

Val Ser Leu Asp Trp Gln Pro Tyr Phe Glu Ala Leu Thr Tyr Gly Gly

245 250 255

Asn Phe His Thr Val Gly Ala Val Leu Thr Pro Leu Ser Val Pro Ala

260 265 270

Phe Thr Leu Ile Ala Gly Asp Arg Ser Val Ser Gly Ser Ala Thr Gly

275 280 285

Thr Pro Tyr Glu Leu Arg Lys Leu Met Arg Phe Ala Ala Arg Ser Lys

290 295 300

Val Ala Pro Thr Thr Glu Leu Phe Pro Met Ser Lys Ile Asn Asp Ala

305 310 315 320

Ile Gln His Val Arg Asp Gly Lys Ala Arg Tyr Arg Val Val Leu Lys

325 330 335

Ala Asp Phe

<210> 23

<211> 315

<212> PRT

<213>Arabidopsis (Arabidopsis thaliana)

<400> 23

Met Ala Asn Ala Ile Thr Phe Phe Lys Leu Asn Thr Gly Ala Lys Phe

1 5 10 15

Pro Ser Val Gly Leu Gly Thr Trp Gln Ala Ser Pro Gly Leu Val Gly

20 25 30

Asp Ala Val Ala Ala Ala Val Lys Ile Gly Tyr Arg His Ile Asp Cys

35 40 45

Ala Gln Ile Tyr Gly Asn Glu Lys Glu Ile Gly Ala Val Leu Lys Lys

50 55 60

Leu Phe Glu Asp Arg Val Val Lys Arg Glu Asp Leu Phe Ile Thr Ser

65 70 75 80

Lys Leu Trp Cys Thr Asp His Asp Pro Gln Asp Val Pro Glu Ala Leu

85 90 95

Asn Arg Thr Leu Lys Asp Leu Gln Leu Glu Tyr Val Asp Leu Tyr Leu

100 105 110

Ile His Trp Pro Ala Arg Ile Lys Lys Gly Ser Val Gly Ile Lys Pro

115 120 125

Glu Asn Leu Leu Pro Val Asp Ile Pro Ser Thr Trp Lys Ala Met Glu

130 135 140

Ala Leu Tyr Asp Ser Gly Lys Ala Arg Ala Ile Gly Val Ser Asn Phe

145 150 155 160

Ser Thr Lys Lys Leu Ala Asp Leu Leu Glu Leu Ala Arg Val Pro Pro

165 170 175

Ala Val Asn Gln Val Glu Cys His Pro Ser Trp Arg Gln Thr Lys Leu

180 185 190

Gln Glu Phe Cys Lys Ser Lys Gly Val His Leu Ser Ala Tyr Ser Pro

195 200 205

Leu Gly Ser Pro Gly Thr Thr Trp Leu Lys Ser Asp Val Leu Lys Asn

210 215 220

Pro Ile Leu Asn Met Val Ala Glu Lys Leu Gly Lys Ser Pro Ala Gln

225 230 235 240

Val Ala Leu Arg Trp Gly Leu Gln Met Gly His Ser Val Leu Pro Lys

245 250 255

Ser Thr Asn Glu Gly Arg Ile Lys Glu Asn Phe Asn Val Phe Asp Trp

260 265 270

Ser Ile Pro Asp Tyr Met Phe Ala Lys Phe Ala Glu Ile Glu Gln Ala

275 280 285

Arg Leu Val Thr Gly Ser Phe Leu Val His Glu Thr Leu Ser Pro Tyr

290 295 300

Lys Ser Ile Glu Glu Leu Trp Asp Gly Glu Ile

305 310 315

<210> 24

<211> 284

<212> PRT

<213>Leishmania donovani (Leishmania donovani)

<400> 24

Met Ala Asp Val Gly Lys Ala Met Val Thr Leu Ser Asn Gly Val Gln

1 5 10 15

Met Pro Gln Leu Gly Leu Gly Val Trp Gln Ser Pro Ala Gly Glu Val

20 25 30

Thr Ala Asn Ala Val Lys Trp Ala Leu Cys Ala Gly Tyr Arg His Ile

35 40 45

Asp Thr Ala Ala Ile Tyr Lys Asn Glu Glu Ser Val Gly Ala Gly Leu

50 55 60

Arg Ala Ser Gly Val Pro Arg Glu Asp Val Phe Ile Thr Thr Lys Leu

65 70 75 80

Trp Asn Thr Glu Gln Gly Tyr Glu Ser Thr Leu Ala Ala Phe Glu Glu

85 90 95

Ser Arg Gln Lys Leu Gly Val Asp Tyr Ile Asp Leu Tyr Leu Ile His

100 105 110

Trp Pro Arg Gly Lys Asp Ile Val Ser Lys Glu Gly Lys Lys Tyr Leu

115 120 125

Asp Ser Trp Arg Ala Phe Glu Gln Leu Tyr Lys Asp Lys Lys Val Arg

130 135 140

Ala Ile Gly Val Ser Asn Phe His Ile His His Leu Glu Asp Val Leu

145 150 155 160

Ala Met Cys Thr Val Thr Pro Met Val Asn Gln Val Glu Leu His Pro

165 170 175

Leu Asn Asn Gln Ala Glu Leu Arg Ala Phe Cys Asp Ala Lys Gln Ile

180 185 190

Lys Val Glu Ala Trp Ser Pro Leu Gly Gln Gly Lys Leu Leu Ser Asn

195 200 205

Pro Ile Leu Ala Ala Ile Gly Ala Lys Tyr Asn Lys Thr Ala Ala Gln

210 215 220

Val Ile Leu Arg Trp Asn Ile Gln Lys Asn Leu Ile Thr Ile Pro Lys

225 230 235 240

Ser Val His Lys Glu Arg Ile Glu Glu Asn Ala Asp Val Phe Asn Phe

245 250 255

Glu Leu Asp Ala Glu Asp Val Met Ser Ile Asp Ala Leu Asn Thr Asn

260 265 270

Ser Arg Tyr Gly Pro Asp Pro Asp Glu Ala Gln Phe

275 280

<210> 25

<211> 349

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 25

Met Lys Ile Lys Ala Val Gly Ala Tyr Ser Ala Lys Gln Pro Leu Glu

1 5 10 15

Pro Met Asp Ile Thr Arg Arg Glu Pro Gly Pro Asn Asp Val Lys Ile

20 25 30

Glu Ile Ala Tyr Cys Gly Val Cys His Ser Asp Leu His Gln Val Arg

35 40 45

Ser Glu Trp Ala Gly Thr Val Tyr Pro Cys Val Pro Gly His Glu Ile

50 55 60

Val Gly Arg Val Val Ala Val Gly Asp Gln Val Glu Lys Tyr Ala Pro

65 70 75 80

Gly Asp Leu Val Gly Val Gly Cys Ile Val Asp Ser Cys Lys His Cys

85 90 95

Glu Glu Cys Glu Asp Gly Leu Glu Asn Tyr Cys Asp His Met Thr Gly

100 105 110

Thr Tyr Asn Ser Pro Thr Pro Asp Glu Pro Gly His Thr Leu Gly Gly

115 120 125

Tyr Ser Gln Gln Ile Val Val His Glu Arg Tyr Val Leu Arg Ile Arg

130 135 140

His Pro Gln Glu Gln Leu Ala Ala Val Ala Pro Leu Leu Cys Ala Gly

145 150 155 160

Ile Thr Thr Tyr Ser Pro Leu Arg His Trp Gln Ala Gly Pro Gly Lys

165 170 175

Lys Val Gly Val Val Gly Ile Gly Gly Leu Gly His Met Gly Ile Lys

180 185 190

Leu Ala His Ala Met Gly Ala His Val Val Ala Phe Thr Thr Ser Glu

195 200 205

Ala Lys Arg Glu Ala Ala Lys Ala Leu Gly Ala Asp Glu Val Val Asn

210 215 220

Ser Arg Asn Ala Asp Glu Met Ala Ala His Leu Lys Ser Phe Asp Phe

225 230 235 240

Ile Leu Asn Thr Val Ala Ala Pro His Asn Leu Asp Asp Phe Thr Thr

245 250 255

Leu Leu Lys Arg Asp Gly Thr Met Thr Leu Val Gly Ala Pro Ala Thr

260 265 270

Pro His Lys Ser Pro Glu Val Phe Asn Leu Ile Met Lys Arg Arg Ala

275 280 285

Ile Ala Gly Ser Met Ile Gly Gly Ile Pro Glu Thr Gln Glu Met Leu

290 295 300

Asp Phe Cys Ala Glu His Gly Ile Val Ala Asp Ile Glu Met Ile Arg

305 310 315 320

Ala Asp Gln Ile Asn Glu Ala Tyr Glu Arg Met Leu Arg Gly Asp Val

325 330 335

Lys Tyr Arg Phe Val Ile Asp Asn Arg Thr Leu Thr Asp

340 345

<210> 26

<211> 346

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 26

Met Gln Tyr His Arg Ile Pro His Ser Ser Leu Glu Val Ser Thr Leu

1 5 10 15

Gly Leu Gly Thr Met Thr Phe Gly Glu Gln Asn Ser Glu Ala Asp Ala

20 25 30

His Ala Gln Leu Asp Tyr Ala Val Ala Gln Gly Ile Asn Leu Ile Asp

35 40 45

Val Ala Glu Met Tyr Pro Val Pro Pro Arg Pro Glu Thr Gln Gly Leu

50 55 60

Thr Glu Thr Tyr Val Gly Asn Trp Leu Ala Lys His Gly Ser Arg Glu

65 70 75 80

Lys Leu Ile Ile Ala Ser Lys Val Ser Gly Pro Ser Arg Asn Asn Asp

85 90 95

Lys Gly Ile Arg Pro Asp Gln Ala Leu Asp Arg Lys Asn Ile Arg Glu

100 105 110

Ala Leu His Asp Ser Leu Lys Arg Leu Gln Thr Asp Tyr Leu Asp Leu

115 120 125

Tyr Gln Val His Trp Pro Gln Arg Pro Thr Asn Cys Phe Gly Lys Leu

130 135 140

Gly Tyr Ser Trp Thr Asp Ser Ala Pro Ala Val Ser Leu Leu Asp Thr

145 150 155 160

Leu Asp Ala Leu Ala Glu Tyr Gln Arg Ala Gly Lys Ile Arg Tyr Ile

165 170 175

Gly Val Ser Asn Glu Thr Ala Phe Gly Val Met Arg Tyr Leu His Leu

180 185 190

Ala Asp Lys His Asp Leu Pro Arg Ile Val Thr Ile Gln Asn Pro Tyr

195 200 205

Ser Leu Leu Asn Arg Ser Phe Glu Val Gly Leu Ala Glu Val Ser Gln

210 215 220

Tyr Glu Gly Val Glu Leu Leu Ala Tyr Ser Cys Leu Gly Phe Gly Thr

225 230 235 240

Leu Thr Gly Lys Tyr Leu Asn Gly Ala Lys Pro Ala Gly Ala Arg Asn

245 250 255

Thr Leu Phe Ser Arg Phe Thr Arg Tyr Ser Gly Glu Gln Thr Gln Lys

260 265 270

Ala Val Ala Ala Tyr Val Asp Ile Ala Arg Arg His Gly Leu Asp Pro

275 280 285

Ala Gln Met Ala Leu Ala Phe Val Arg Arg Gln Pro Phe Val Ala Ser

290 295 300

Thr Leu Leu Gly Ala Thr Thr Met Asp Gln Leu Lys Thr Asn Ile Glu

305 310 315 320

Ser Leu His Leu Glu Leu Ser Glu Asp Val Leu Ala Glu Ile Glu Ala

325 330 335

Val His Gln Val Tyr Thr Tyr Pro Ala Pro

340 345

<210> 27

<211> 399

<212> PRT

<213>Ethanolicus (Thermoanaerobacter ethanolicus)

<400> 27

Met Trp Glu Thr Lys Ile Asn Pro Asn Lys Val Phe Glu Leu Arg Cys

1 5 10 15

Lys Asn Thr Thr Tyr Phe Gly Ile Gly Ser Ile Lys Lys Ile Lys Asp

20 25 30

Ile Leu Glu Val Leu Lys Asn Lys Gly Ile Asn Asn Val Ile Leu Val

35 40 45

Thr Gly Lys Gly Ser Tyr Lys Ala Ser Gly Ala Trp Asp Val Val Lys

50 55 60

Pro Ala Leu Glu Thr Leu Gly Phe Lys Tyr Ser Leu Tyr Asp Lys Val

65 70 75 80

Gly Pro Asn Pro Thr Val Asp Met Ile Asp Glu Ala Ala Lys Ile Gly

85 90 95

Arg Glu Thr Gly Ala Lys Ala Val Ile Gly Ile Gly Gly Gly Ser Pro

100 105 110

Ile Asp Thr Ala Lys Ser Val Ala Val Leu Leu Glu Tyr Thr Asp Lys

115 120 125

Asn Ala Arg Glu Leu Tyr Glu Gln Lys Phe Ile Pro Glu Lys Ala Ala

130 135 140

Pro Ile Ile Ala Ile Asn Leu Thr His Gly Thr Gly Thr Glu Val Asp

145 150 155 160

Arg Phe Ala Val Ala Thr Ile Pro Glu Lys Asn Tyr Lys Pro Ala Ile

165 170 175

Ala Tyr Asp Cys Leu Tyr Pro Met Tyr Ala Ile Asp Asp Pro Ser Leu

180 185 190

Met Thr Lys Leu Asp Lys Lys Gln Thr Ile Ala Val Thr Ile Asp Ala

195 200 205

Leu Asn His Val Thr Glu Ala Ala Thr Thr Leu Val Ala Ser Pro Tyr

210 215 220

Ser Val Leu Met Ala Lys Glu Thr Val Arg Leu Ile Val Arg Tyr Leu

225 230 235 240

Pro Ala Ala Val Asn Asp Pro Glu Asn Leu Val Ala Arg Tyr Tyr Leu

245 250 255

Leu Tyr Ala Ser Ala Leu Ala Gly Ile Ser Phe Asp Asn Gly Leu Leu

260 265 270

His Leu Thr His Ala Leu Glu His Pro Leu Ser Ala Val Lys Pro Glu

275 280 285

Ile Ala His Gly Leu Gly Leu Gly Ala Ile Leu Pro Ala Val Val Lys

290 295 300

Ala Ile Tyr Pro Ser Val Ala Glu Val Leu Ala Glu Val Tyr Ser Pro

305 310 315 320

Ile Val Pro Gly Leu Lys Gly Leu Pro Ala Glu Ala Glu Tyr Val Ala

325 330 335

Lys Lys Val Glu Glu Trp Leu Phe Lys Val Gly Cys Thr Gln Lys Leu

340 345 350

Ser Asp Phe Gly Phe Thr Lys Glu Asp Ile Pro Thr Leu Val Arg Leu

355 360 365

Ala Lys Thr Thr Pro Ser Leu Asp Gly Leu Leu Ser Asn Ala Pro Val

370 375 380

Glu Ala Thr Glu Ala Val Ile Ala Lys Ile Tyr Glu Glu Ser Phe

385 390 395

<210> 28

<211> 346

<212> PRT

<213>Bacillus subtilis (Bacillus subtilis)

<400> 28

Met Lys Ala Ala Arg Trp His Asn Gln Lys Asp Ile Arg Ile Glu His

1 5 10 15

Ile Glu Glu Pro Lys Thr Glu Pro Gly Lys Val Lys Ile Lys Val Lys

20 25 30

Trp Cys Gly Ile Cys Gly Ser Asp Leu His Glu Tyr Leu Gly Gly Pro

35 40 45

Ile Phe Ile Pro Val Asp Lys Pro His Pro Leu Thr Asn Glu Thr Ala

50 55 60

Pro Val Thr Met Gly His Glu Phe Ser Gly Glu Val Val Glu Val Gly

65 70 75 80

Glu Gly Val Glu Asn Tyr Lys Val Gly Asp Arg Val Val Val Glu Pro

85 90 95

Ile Phe Ala Thr His Gly His Gln Gly Ala Tyr Asn Leu Asp Glu Gln

100 105 110

Met Gly Phe Leu Gly Leu Ala Gly Gly Gly Gly Gly Phe Ser Glu Tyr

115 120 125

Val Ser Val Asp Glu Glu Leu Leu Phe Lys Leu Pro Asp Glu Leu Ser

130 135 140

Tyr Glu Gln Gly Ala Leu Val Glu Pro Ser Ala Val Ala Leu Tyr Ala

145 150 155 160

Val Arg Ser Ser Lys Leu Lys Ala Gly Asp Lys Ala Ala Val Phe Gly

165 170 175

Cys Gly Pro Ile Gly Leu Leu Val Ile Glu Ala Leu Lys Ala Ala Gly

180 185 190

Ala Thr Asp Ile Tyr Ala Val Glu Leu Ser Pro Glu Arg Gln Gln Lys

195 200 205

Ala Glu Glu Leu Gly Ala Ile Ile Val Asp Pro Ser Lys Thr Asp Asp

210 215 220

Val Val Ala Glu Ile Ala Glu Arg Thr Gly Gly Gly Val Asp Val Ala

225 230 235 240

Phe Glu Val Thr Gly Val Pro Val Val Leu Arg Gln Ala Ile Gln Ser

245 250 255

Thr Thr Ile Ala Gly Glu Thr Val Ile Val Ser Ile Trp Glu Lys Gly

260 265 270

Ala Glu Ile His Pro Asn Asp Ile Val Ile Lys Glu Arg Thr Val Lys

275 280 285

Gly Ile Ile Gly Tyr Arg Asp Ile Phe Pro Ala Val Leu Ser Leu Met

290 295 300

Lys Glu Gly Tyr Phe Ser Ala Asp Lys Leu Val Thr Lys Lys Ile Val

305 310 315 320

Leu Asp Asp Leu Ile Glu Glu Gly Phe Gly Ala Leu Ile Lys Glu Lys

325 330 335

Ser Gln Val Lys Ile Leu Val Arg Pro Asn

340 345

<210> 29

<211> 382

<212> PRT

<213>Saccharomyces cerevisiae (Saccharomyces cerevisiae)

<400> 29

Met Arg Ala Leu Ala Tyr Phe Lys Lys Gly Asp Ile His Phe Thr Asn

1 5 10 15

Asp Ile Pro Arg Pro Glu Ile Gln Thr Asp Asp Glu Val Ile Ile Asp

20 25 30

Val Ser Trp Cys Gly Ile Cys Gly Ser Asp Leu His Glu Tyr Leu Asp

35 40 45

Gly Pro Ile Phe Met Pro Lys Asp Gly Glu Cys His Lys Leu Ser Asn

50 55 60

Ala Ala Leu Pro Leu Ala Met Gly His Glu Met Ser Gly Ile Val Ser

65 70 75 80

Lys Val Gly Pro Lys Val Thr Lys Val Lys Val Gly Asp His Val Val

85 90 95

Val Asp Ala Ala Ser Ser Cys Ala Asp Leu His Cys Trp Pro His Ser

100 105 110

Lys Phe Tyr Asn Ser Lys Pro Cys Asp Ala Cys Gln Arg Gly Ser Glu

115 120 125

Asn Leu Cys Thr His Ala Gly Phe Val Gly Leu Gly Val Ile Ser Gly

130 135 140

Gly Phe Ala Glu Gln Val Val Val Ser Gln His His Ile Ile Pro Val

145 150 155 160

Pro Lys Glu Ile Pro Leu Asp Val Ala Ala Leu Val Glu Pro Leu Ser

165 170 175

Val Thr Trp His Ala Val Lys Ile Ser Gly Phe Lys Lys Gly Ser Ser

180 185 190

Ala Leu Val Leu Gly Ala Gly Pro Ile Gly Leu Cys Thr Ile Leu Val

195 200 205

Leu Lys Gly Met Gly Ala Ser Lys Ile Val Val Ser Glu Ile Ala Glu

210 215 220

Arg Arg Ile Glu Met Ala Lys Lys Leu Gly Val Glu Val Phe Asn Pro

225 230 235 240

Ser Lys His Gly His Lys Ser Ile Glu Ile Leu Arg Gly Leu Thr Lys

245 250 255

Ser His Asp Gly Phe Asp Tyr Ser Tyr Asp Cys Ser Gly Ile Gln Val

260 265 270

Thr Phe Glu Thr Ser Leu Lys Ala Leu Thr Phe Lys Gly Thr Ala Thr

275 280 285

Asn Ile Ala Val Trp Gly Pro Lys Pro Val Pro Phe Gln Pro Met Asp

290 295 300

Val Thr Leu Gln Glu Lys Val Met Thr Gly Ser Ile Gly Tyr Val Val

305 310 315 320

Glu Asp Phe Glu Glu Val Val Arg Ala Ile His Asn Gly Asp Ile Ala

325 330 335

Met Glu Asp Cys Lys Gln Leu Ile Thr Gly Lys Gln Arg Ile Glu Asp

340 345 350

Gly Trp Glu Lys Gly Phe Gln Glu Leu Met Asp His Lys Glu Ser Asn

355 360 365

Val Lys Ile Leu Leu Thr Pro Asn Asn His Gly Glu Met Lys

370 375 380

<210> 30

<211> 258

<212> PRT

<213>Corynebacterium glutamicum (Corynebacterium glutamicum)

<400> 30

Met Ser Lys Val Ala Met Val Thr Gly Gly Ala Gln Gly Ile Gly Arg

1 5 10 15

Gly Ile Ser Glu Lys Leu Ala Ala Asp Gly Phe Asp Ile Ala Val Ala

20 25 30

Asp Leu Pro Gln Gln Glu Glu Gln Ala Ala Glu Thr Ile Lys Leu Ile

35 40 45

Glu Ala Ala Gly Gln Lys Ala Val Phe Val Gly Leu Asp Val Thr Asp

50 55 60

Lys Ala Asn Phe Asp Ser Ala Ile Asp Glu Ala Ala Glu Lys Leu Gly

65 70 75 80

Gly Phe Asp Val Leu Val Asn Asn Ala Gly Ile Ala Gln Ile Lys Pro

85 90 95

Leu Leu Glu Val Thr Glu Glu Asp Leu Lys Gln Ile Tyr Ser Val Asn

100 105 110

Val Phe Ser Val Phe Phe Gly Ile Gln Ala Ala Ser Arg Lys Phe Asp

115 120 125

Glu Leu Gly Val Lys Gly Lys Ile Ile Asn Ala Ala Ser Ile Ala Ala

130 135 140

Ile Gln Gly Phe Pro Ile Leu Ser Ala Tyr Ser Thr Thr Lys Phe Ala

145 150 155 160

Val Arg Gly Leu Thr Gln Ala Ala Ala Gln Glu Leu Ala Pro Lys Gly

165 170 175

His Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Gly Thr Gly Met Trp

180 185 190

Glu Gln Ile Asp Ala Glu Leu Ser Lys Ile Asn Gly Lys Pro Ile Gly

195 200 205

Glu Asn Phe Lys Glu Tyr Ser Ser Ser Ile Ala Leu Gly Arg Pro Ser

210 215 220

Val Pro Glu Asp Val Ala Gly Leu Val Ser Phe Leu Ala Ser Glu Asn

225 230 235 240

Ser Asn Tyr Ile Thr Gly Gln Val Met Leu Val Asp Gly Gly Met Leu

245 250 255

Tyr Asn

<210> 31

<211> 256

<212> PRT

<213>Friedlander's bacillus (Klebsiella pneumoniae)

<400> 31

Met Lys Lys Val Ala Leu Val Thr Gly Ala Gly Gln Gly Ile Gly Lys

1 5 10 15

Ala Ile Ala Leu Arg Leu Val Lys Asp Gly Phe Ala Val Ala Ile Ala

20 25 30

Asp Tyr Asn Asp Ala Thr Ala Lys Ala Val Ala Ser Glu Ile Asn Gln

35 40 45

Ala Gly Gly Arg Ala Met Ala Val Lys Val Asp Val Ser Asp Arg Asp

50 55 60

Gln Val Phe Ala Ala Val Glu Gln Ala Arg Lys Thr Leu Gly Gly Phe

65 70 75 80

Asp Val Ile Val Asn Asn Ala Gly Val Ala Pro Ser Thr Pro Ile Glu

85 90 95

Ser Ile Thr Pro Glu Ile Val Asp Lys Val Tyr Asn Ile Asn Val Lys

100 105 110

Gly Val Ile Trp Gly Ile Gln Ala Ala Val Glu Ala Phe Lys Lys Glu

115 120 125

Gly His Gly Gly Lys Ile Ile Asn Ala Cys Ser Gln Ala Gly His Val

130 135 140

Gly Asn Pro Glu Leu Ala Val Tyr Ser Ser Ser Lys Phe Ala Val Arg

145 150 155 160

Gly Leu Thr Gln Thr Ala Ala Arg Asp Leu Ala Pro Leu Gly Ile Thr

165 170 175

Val Asn Gly Tyr Cys Pro Gly Ile Val Lys Thr Pro Met Trp Ala Glu

180 185 190

Ile Asp Arg Gln Val Ser Glu Ala Ala Gly Lys Pro Leu Gly Tyr Gly

195 200 205

Thr Ala Glu Phe Ala Lys Arg Ile Thr Leu Gly Arg Leu Ser Glu Pro

210 215 220

Glu Asp Val Ala Ala Cys Val Ser Tyr Leu Ala Ser Pro Asp Ser Asp

225 230 235 240

Tyr Met Thr Gly Gln Ser Leu Leu Ile Asp Gly Gly Met Val Phe Asn

245 250 255

<210> 32

<211> 251

<212> PRT

<213>Serratia marcescens (Serratia marcescens)

<400> 32

Met Arg Phe Asp Asn Lys Val Val Val Ile Thr Gly Ala Gly Thr Gly

1 5 10 15

Met Gly Glu Ala Ala Ala Arg Arg Phe Ser Ala Glu Gly Ala Ile Val

20 25 30

Val Leu Ala Asp Trp Ala Lys Glu Ala Val Asp Lys Val Ala Ala Ser

35 40 45

Leu Pro Lys Gly Arg Ala Met Ala Val His Ile Asp Val Ser Asp His

50 55 60

Val Ala Val Glu Lys Met Met Asn Glu Val Ala Glu Lys Leu Gly Arg

65 70 75 80

Ile Asp Val Leu Leu Asn Asn Ala Gly Val His Val Ala Gly Ser Val

85 90 95

Leu Glu Thr Ser Val Asp Asp Trp Arg Arg Ile Ala Gly Val Asp Ile

100 105 110

Asp Gly Val Val Phe Cys Ser Lys Phe Ala Leu Pro His Leu Leu Lys

115 120 125

Thr Lys Gly Cys Ile Val Asn Thr Ala Ser Val Ser Gly Leu Gly Gly

130 135 140

Asp Trp Gly Ala Ala Tyr Tyr Cys Ala Ala Lys Gly Ala Val Val Asn

145 150 155 160

Leu Thr Arg Ala Met Ala Leu Asp His Gly Gly Asp Gly Val Arg Ile

165 170 175

Asn Ser Val Cys Pro Ser Leu Val Lys Thr Asn Met Thr Asn Gly Trp

180 185 190

Pro Gln Glu Ile Arg Asp Lys Phe Asn Glu Arg Ile Ala Leu Gly Arg

195 200 205

Ala Ala Glu Pro Glu Glu Val Ala Ala Val Met Ala Phe Leu Ala Ser

210 215 220

Asp Asp Ala Ser Phe Ile Asn Gly Ala Asn Ile Pro Val Asp Gly Gly

225 230 235 240

Ala Thr Ala Ser Asp Gly Gln Gln Asn Ile Val

245 250

<210> 33

<211> 367

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 33

Met Asp Arg Ile Ile Gln Ser Pro Gly Lys Tyr Ile Gln Gly Ala Asp

1 5 10 15

Val Ile Asn Arg Leu Gly Glu Tyr Leu Lys Pro Leu Ala Glu Arg Trp

20 25 30

Leu Val Val Gly Asp Lys Phe Val Leu Gly Phe Ala Gln Ser Thr Val

35 40 45

Glu Lys Ser Phe Lys Asp Ala Gly Leu Val Val Glu Ile Ala Pro Phe

50 55 60

Gly Gly Glu Cys Ser Gln Asn Glu Ile Asp Arg Leu Arg Gly Ile Ala

65 70 75 80

Glu Thr Ala Gln Cys Gly Ala Ile Leu Gly Ile Gly Gly Gly Lys Thr

85 90 95

Leu Asp Thr Ala Lys Ala Leu Ala His Phe Met Gly Val Pro Val Ala

100 105 110

Ile Ala Pro Thr Ile Ala Ser Thr Asp Ala Pro Cys Ser Ala Leu Ser

115 120 125

Val Ile Tyr Thr Asp Glu Gly Glu Phe Asp Arg Tyr Leu Leu Leu Pro

130 135 140

Asn Asn Pro Asn Met Val Ile Val Asp Thr Lys Ile Val Ala Gly Ala

145 150 155 160

Pro Ala Arg Leu Leu Ala Ala Gly Ile Gly Asp Ala Leu Ala Thr Trp

165 170 175

Phe Glu Ala Arg Ala Cys Ser Arg Ser Gly Ala Thr Thr Met Ala Gly

180 185 190

Gly Lys Cys Thr Gln Ala Ala Leu Ala Leu Ala Glu Leu Cys Tyr Asn

195 200 205

Thr Leu Leu Glu Glu Gly Glu Lys Ala Met Leu Ala Ala Glu Gln His

210 215 220

Val Val Thr Pro Ala Leu Glu Arg Val Ile Glu Ala Asn Thr Tyr Leu

225 230 235 240

Ser Gly Val Gly Phe Glu Ser Gly Gly Leu Ala Ala Ala His Ala Val

245 250 255

His Asn Gly Leu Thr Ala Ile Pro Asp Ala His His Tyr Tyr His Gly

260 265 270

Glu Lys Val Ala Phe Gly Thr Leu Thr Gln Leu Val Leu Glu Asn Ala

275 280 285

Pro Val Glu Glu Ile Glu Thr Val Ala Ala Leu Ser His Ala Val Gly

290 295 300

Leu Pro Ile Thr Leu Ala Gln Leu Asp Ile Lys Glu Asp Val Pro Ala

305 310 315 320

Lys Met Arg Ile Val Ala Glu Ala Ala Cys Ala Glu Gly Glu Thr Ile

325 330 335

His Asn Met Pro Gly Gly Ala Thr Pro Asp Gln Val Tyr Ala Ala Leu

340 345 350

Leu Val Ala Asp Gln Tyr Gly Gln Arg Phe Leu Gln Glu Trp Glu

355 360 365

<210> 34

<211> 258

<212> PRT

<213>Corynebacterium glutamicum (Corynebacterium glutamicum)

<400> 34

Met Ser Lys Val Ala Met Val Thr Gly Gly Ala Gln Gly Ile Gly Arg

1 5 10 15

Gly Ile Ser Glu Lys Leu Ala Ala Asp Gly Phe Asp Ile Ala Val Ala

20 25 30

Asp Leu Pro Gln Gln Glu Glu Gln Ala Ala Glu Thr Ile Lys Leu Ile

35 40 45

Glu Ala Ala Asp Gln Lys Ala Val Phe Val Gly Leu Asp Val Thr Asp

50 55 60

Lys Ala Asn Phe Asp Ser Ala Ile Asp Glu Ala Ala Glu Lys Leu Gly

65 70 75 80

Gly Phe Asp Val Leu Val Asn Asn Ala Gly Ile Ala Gln Ile Lys Pro

85 90 95

Leu Leu Glu Val Thr Glu Glu Asp Leu Lys Gln Ile Tyr Ser Val Asn

100 105 110

Val Phe Ser Val Phe Phe Gly Ile Gln Ala Ala Ser Arg Lys Phe Asp

115 120 125

Glu Leu Gly Val Lys Gly Lys Ile Ile Asn Ala Ala Ser Ile Ala Ala

130 135 140

Ile Gln Gly Phe Pro Ile Leu Ser Ala Tyr Ser Thr Thr Lys Phe Ala

145 150 155 160

Val Arg Gly Leu Thr Gln Ala Ala Ala Gln Glu Leu Ala Pro Lys Gly

165 170 175

His Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Gly Thr Gly Met Trp

180 185 190

Glu Gln Ile Asp Ala Glu Leu Ser Lys Ile Asn Gly Lys Pro Ile Gly

195 200 205

Glu Asn Phe Lys Glu Tyr Ser Ser Ser Ile Ala Leu Gly Arg Pro Ser

210 215 220

Val Pro Glu Asp Val Ala Gly Leu Val Ser Phe Leu Ala Ser Glu Asn

225 230 235 240

Ser Asn Tyr Val Thr Gly Gln Val Met Leu Val Asp Gly Gly Met Leu

245 250 255

Tyr Asn

<210> 35

<211> 318

<212> PRT

<213>Hypocrea jecorina (Hypocrea jecorina)

<400> 35

Met Ala Ser Lys Thr Tyr Thr Leu Asn Thr Gly Ala Lys Ile Pro Ala

1 5 10 15

Val Gly Phe Gly Thr Phe Ala Asn Glu Gly Ala Lys Gly Glu Thr Tyr

20 25 30

Ala Ala Val Thr Lys Ala Leu Asp Val Gly Tyr Arg His Leu Asp Cys

35 40 45

Ala Trp Phe Tyr His Asn Glu Asp Glu Val Gly Asp Ala Val Arg Asp

50 55 60

Phe Leu Ala Arg Arg Pro Asp Val Lys Arg Glu Asp Leu Phe Ile Cys

65 70 75 80

Thr Lys Val Trp Asn His Leu His Glu Pro Glu Asp Val Lys Trp Ser

85 90 95

Ala Lys Asn Ser Cys Glu Asn Leu Lys Val Asp Tyr Ile Asp Leu Phe

100 105 110

Leu Val His Trp Pro Ile Ala Ala Glu Lys Asn Ser Asp Arg Ser Val

115 120 125

Lys Leu Gly Pro Asp Gly Lys Tyr Val Ile Asn Gln Ala Leu Thr Glu

130 135 140

Asn Pro Glu Pro Thr Trp Arg Ala Met Glu Glu Leu Val Glu Ser Gly

145 150 155 160

Leu Val Lys Ala Ile Gly Val Ser Asn Trp Thr Ile Pro Gly Leu Lys

165 170 175

Lys Leu Leu Gln Ile Ala Lys Ile Lys Pro Ala Val Asn Gln Ile Glu

180 185 190

Ile His Pro Phe Leu Pro Asn Glu Glu Leu Val Ala Phe Cys Phe Glu

195 200 205

Asn Gly Ile Leu Pro Glu Ala Tyr Ser Pro Leu Gly Ser Gln Asn Gln

210 215 220

Val Pro Ser Thr Gly Glu Arg Val Arg Asp Asn Pro Thr Leu Lys Ala

225 230 235 240

Val Ala Glu Arg Ser Gly Tyr Ser Leu Ala Gln Ile Leu Leu Ala Trp

245 250 255

Gly Leu Lys Arg Gly Tyr Val Val Leu Pro Lys Ser Ser Thr Pro Ser

260 265 270

Arg Ile Glu Ser Asn Phe Asn Ile Pro Glu Leu Ser Asp Glu Asp Phe

275 280 285

Glu Ala Ile Gln Gln Val Ala Lys Gly Arg His Thr Arg Phe Val Asn

290 295 300

Met Lys Asp Thr Phe Gly Tyr Asn Val Trp Pro Glu Glu Glu

305 310 315

<210> 36

<211> 385

<212> PRT

<213>Clostridium butyricum (Clostridium butyricum)

<400> 36

Met Arg Met Tyr Asp Tyr Leu Val Pro Ser Val Asn Phe Met Gly Ala

1 5 10 15

Asn Ser Val Ser Val Val Gly Glu Arg Cys Lys Ile Leu Gly Gly Lys

20 25 30

Lys Ala Leu Ile Val Thr Asp Lys Phe Leu Lys Asp Met Glu Gly Gly

35 40 45

Ala Val Glu Leu Thr Val Lys Tyr Leu Lys Glu Ala Gly Leu Asp Val

50 55 60

Val Tyr Tyr Asp Gly Val Glu Pro Asn Pro Lys Asp Val Asn Val Ile

65 70 75 80

Glu Gly Leu Lys Ile Phe Lys Glu Glu Asn Cys Asp Met Ile Val Thr

85 90 95

Val Gly Gly Gly Ser Ser His Asp Cys Gly Lys Gly Ile Gly Ile Ala

100 105 110

Ala Thr His Glu Gly Asp Leu Tyr Asp Tyr Ala Gly Ile Glu Thr Leu

115 120 125

Val Asn Pro Leu Pro Pro Ile Val Ala Val Asn Thr Thr Ala Gly Thr

130 135 140

Ala Ser Glu Leu Thr Arg His Cys Val Leu Thr Asn Thr Lys Lys Lys

145 150 155 160

Ile Lys Phe Val Ile Val Ser Trp Arg Asn Leu Pro Leu Val Ser Ile

165 170 175

Asn Asp Pro Met Leu Met Val Lys Lys Pro Ala Gly Leu Thr Ala Ala

180 185 190

Thr Gly Met Asp Ala Leu Thr His Ala Ile Glu Ala Tyr Val Ser Lys

195 200 205

Asp Ala Asn Pro Val Thr Asp Ala Ser Ala Ile Gln Ala Ile Lys Leu

210 215 220

Ile Ser Gln Asn Leu Arg Gln Ala Val Ala Leu Gly Glu Asn Leu Glu

225 230 235 240

Ala Arg Glu Asn Met Ala Tyr Ala Ser Leu Leu Ala Gly Met Ala Phe

245 250 255

Asn Asn Ala Asn Leu Gly Tyr Val His Ala Met Ala His Gln Leu Gly

260 265 270

Gly Leu Tyr Asp Met Pro His Gly Val Ala Asn Ala Met Leu Leu Pro

275 280 285

His Val Glu Arg Tyr Asn Met Leu Ser Asn Pro Lys Lys Phe Ala Asp

290 295 300

Ile Ala Glu Phe Met Gly Glu Asn Ile Ser Gly Leu Ser Val Met Glu

305 310 315 320

Ala Ala Glu Lys Ala Ile Asn Ala Met Phe Arg Leu Ser Glu Asp Val

325 330 335

Gly Ile Pro Lys Ser Leu Lys Glu Met Gly Val Lys Gln Glu Asp Phe

340 345 350

Glu His Met Ala Glu Leu Ala Leu Leu Asp Gly Asn Ala Phe Ser Asn

355 360 365

Pro Arg Lys Gly Asn Ala Lys Asp Ile Ile Asn Ile Phe Lys Ala Ala

370 375 380

Tyr

385

<210> 37

<211> 387

<212> PRT

<213>Citrobacter freundii (Citrobacter Freundii)

<400> 37

Met Ser Tyr Arg Met Phe Asp Tyr Leu Val Pro Asn Val Asn Phe Phe

1 5 10 15

Gly Pro Asn Ala Ile Ser Val Val Gly Glu Arg Cys Lys Leu Leu Gly

20 25 30

Gly Lys Lys Ala Leu Leu Val Thr Asp Lys Gly Leu Arg Ala Ile Lys

35 40 45

Asp Gly Ala Val Asp Lys Thr Leu Thr His Leu Arg Glu Ala Gly Ile

50 55 60

Asp Val Val Val Phe Asp Gly Val Glu Pro Asn Pro Lys Asp Thr Asn

65 70 75 80

Val Arg Asp Gly Leu Glu Val Phe Arg Lys Glu His Cys Asp Ile Ile

85 90 95

Val Thr Val Gly Gly Gly Ser Pro His Asp Cys Gly Lys Gly Ile Gly

100 105 110

Ile Ala Ala Thr His Glu Gly Asp Leu Tyr Ser Tyr Ala Gly Ile Glu

115 120 125

Thr Leu Thr Asn Pro Leu Pro Pro Ile Val Ala Val Asn Thr Thr Ala

130 135 140

Gly Thr Ala Ser Glu Val Thr Arg His Cys Val Leu Thr Asn Thr Lys

145 150 155 160

Thr Lys Val Lys Phe Val Ile Val Ser Trp Arg Asn Leu Pro Ser Val

165 170 175

Ser Ile Asn Asp Pro Leu Leu Met Leu Gly Lys Pro Ala Pro Leu Thr

180 185 190

Ala Ala Thr Gly Met Asp Ala Leu Thr His Ala Val Glu Ala Tyr Ile

195 200 205

Ser Lys Asp Ala Asn Pro Val Thr Asp Ala Ala Ala Ile Gln Ala Ile

210 215 220

Arg Leu Ile Ala Arg Asn Leu Arg Gln Ala Val Ala Leu Gly Ser Asn

225 230 235 240

Leu Lys Ala Arg Glu Asn Met Ala Tyr Ala Ser Leu Leu Ala Gly Met

245 250 255

Ala Phe Asn Asn Ala Asn Leu Gly Tyr Val His Ala Met Ala His Gln

260 265 270

Leu Gly Gly Leu Tyr Asp Met Pro His Gly Val Ala Asn Ala Val Leu

275 280 285

Leu Pro His Val Ala Arg Tyr Asn Leu Ile Ala Asn Pro Glu Lys Phe

290 295 300

Ala Asp Ile Ala Glu Phe Met Gly Glu Asn Thr Asp Gly Leu Ser Thr

305 310 315 320

Met Asp Ala Ala Glu Leu Ala Ile His Ala Ile Ala Arg Leu Ser Ala

325 330 335

Asp Ile Gly Ile Pro Gln His Leu Arg Asp Leu Gly Val Lys Glu Ala

340 345 350

Asp Phe Pro Tyr Met Ala Glu Met Ala Leu Lys Asp Gly Asn Ala Phe

355 360 365

Ser Asn Pro Arg Lys Gly Asn Glu Lys Glu Ile Ala Glu Ile Phe Arg

370 375 380

Gln Ala Phe

385

<210> 38

<211> 387

<212> PRT

<213>Friedlander's bacillus (Klebsiella pneumoniae)

<400> 38

Met Ser Tyr Arg Met Phe Asp Tyr Leu Val Pro Asn Val Asn Phe Phe

1 5 10 15

Gly Pro Asn Ala Ile Ser Val Val Gly Glu Arg Cys Gln Leu Leu Gly

20 25 30

Gly Lys Lys Ala Leu Leu Val Thr Asp Lys Gly Leu Arg Ala Ile Lys

35 40 45

Asp Gly Ala Val Asp Lys Thr Leu His Tyr Leu Arg Glu Ala Gly Ile

50 55 60

Glu Val Ala Ile Phe Asp Gly Val Glu Pro Asn Pro Lys Asp Thr Asn

65 70 75 80

Val Arg Asp Gly Leu Ala Val Phe Arg Arg Glu Gln Cys Asp Ile Ile

85 90 95

Val Thr Val Gly Gly Gly Ser Pro His Asp Cys Gly Lys Gly Ile Gly

100 105 110

Ile Ala Ala Thr His Glu Gly Asp Leu Tyr Gln Tyr Ala Gly Ile Glu

115 120 125

Thr Leu Thr Asn Pro Leu Pro Pro Ile Val Ala Val Asn Thr Thr Ala

130 135 140

Gly Thr Ala Ser Glu Val Thr Arg His Cys Val Leu Thr Asn Thr Glu

145 150 155 160

Thr Lys Val Lys Phe Val Ile Val Ser Trp Arg Asn Leu Pro Ser Val

165 170 175

Ser Ile Asn Asp Pro Leu Leu Met Ile Gly Lys Pro Ala Ala Leu Thr

180 185 190

Ala Ala Thr Gly Met Asp Ala Leu Thr His Ala Val Glu Ala Tyr Ile

195 200 205

Ser Lys Asp Ala Asn Pro Val Thr Asp Ala Ala Ala Met Gln Ala Ile

210 215 220

Arg Leu Ile Ala Arg Asn Leu Arg Gln Ala Val Ala Leu Gly Ser Asn

225 230 235 240

Leu Gln Ala Arg Glu Asn Met Ala Tyr Ala Ser Leu Leu Ala Gly Met

245 250 255

Ala Phe Asn Asn Ala Asn Leu Gly Tyr Val His Ala Met Ala His Gln

260 265 270

Leu Gly Gly Leu Tyr Asp Met Pro His Gly Val Ala Asn Ala Val Leu

275 280 285

Leu Pro His Val Ala Arg Tyr Asn Leu Ile Ala Asn Pro Glu Lys Phe

290 295 300

Ala Asp Ile Ala Glu Leu Met Gly Glu Asn Ile Thr Gly Leu Ser Thr

305 310 315 320

Leu Asp Ala Ala Glu Lys Ala Ile Ala Ala Ile Thr Arg Leu Ser Met

325 330 335

Asp Ile Gly Ile Pro Gln His Leu Arg Asp Leu Gly Val Lys Glu Ala

340 345 350

Asp Phe Pro Tyr Met Ala Glu Met Ala Leu Lys Asp Gly Asn Ala Phe

355 360 365

Ser Asn Pro Arg Lys Gly Asn Glu Gln Glu Ile Ala Ala Ile Phe Arg

370 375 380

Gln Ala Phe

385

<210> 39

<211> 22

<212> PRT

<213>Rhodococcus erythropolis (Rhodoccus erythropolis)

<400> 39

Met Lys Thr Lys Ala Ala Val Leu Phe Glu Thr His Lys Pro Phe Glu

1 5 10 15

Ile Val Glu Leu Glu Leu

20

<210> 40

<211> 371

<212> PRT

<213>Methanol amycolatosis (Amycolatopsis methanolica)

<400> 40

Met Lys Thr Lys Ala Ala Val Leu His Ser Ala Gly Lys Pro Phe Glu

1 5 10 15

Ile Glu Glu Leu Glu Leu Asp Gly Pro Arg Glu Gly Glu Val Leu Ile

20 25 30

Lys Tyr Thr Ala Ala Gly Leu Cys His Ser Asp Leu His Leu Ile Asp

35 40 45

Asn Asp Leu Val Pro Arg Phe Pro Ile Val Gly Gly His Glu Gly Ala

50 55 60

Gly Val Ile Glu Asp Val Gly Pro Gly Val Thr Lys Val Lys Pro Gly

65 70 75 80

Asp His Val Val Cys Ser Phe Ile Pro Asn Cys Gly Thr Cys Arg Tyr

85 90 95

Cys Ala Thr Gly Arg Ser Asn Leu Cys Asp Met Gly Ala Thr Ile Leu

100 105 110

Asp Gly Gly Met Pro Asp Gly Ser Phe Arg Phe His Arg Gly Gly Thr

115 120 125

Asp Tyr Gly Ala Met Cys Met Leu Gly Thr Phe Ser Glu Arg Ala Thr

130 135 140

Ile Ser Gln His Ser Val Val Lys Val Asp Asp Trp Leu Pro Leu Glu

145 150 155 160

Thr Ala Val Leu Val Gly Cys Gly Val Pro Thr Gly Trp Ala Ser Ala

165 170 175

Asn Tyr Ala Gly Gly Val Arg Ala Gly Asp Thr Cys Val Val Tyr Gly

180 185 190

Ile Gly Gly Ile Gly Ile Asn Ala Val Gln Gly Ala Ala His Ala Gly

195 200 205

Ala Ala Asn Val Ile Ala Val Asp Pro Val Ala Phe Lys Arg Glu Lys

210 215 220

Ala Leu Glu Leu Gly Ala Thr His Ala Phe Ala Ser Ala Asp Glu Ala

225 230 235 240

Ala Ala Lys Val Ala Glu Leu Thr Trp Gly Gln Met Ala Asp Gln Ala

245 250 255

Leu Ile Thr Val Gly Thr Val Val Glu Gln Val Val Thr Asp Ala Phe

260 265 270

Asn Val Ile Gly Lys Gly Gly Thr Val Val Ile Thr Gly Leu Ala Asn

275 280 285

Pro Glu Lys Leu Thr Val His Leu Ser Gly Gly Val Met Thr Leu Phe

290 295 300

Glu Lys Thr Val Lys Gly Thr Leu Phe Gly Ser Ala Asn Pro Gln Tyr

305 310 315 320

Asp Ile Val Arg Leu Leu Arg Leu Tyr Gln Ala Gly His Val Lys Leu

325 330 335

Asp Glu Leu Val Thr Lys Arg Tyr Ser Leu Glu Glu Val Asn Glu Gly

340 345 350

Tyr Gln Asp Leu Arg Asp Gly Lys Asn Ile Arg Gly Val Ile Met His

355 360 365

Ser Ala Asp

370

<210> 41

<211> 382

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 41

Met Ala Asn Arg Met Ile Leu Asn Glu Thr Ala Trp Phe Gly Arg Gly

1 5 10 15

Ala Val Gly Ala Leu Thr Asp Glu Val Lys Arg Arg Gly Tyr Gln Lys

20 25 30

Ala Leu Ile Val Thr Asp Lys Thr Leu Val Gln Cys Gly Val Val Ala

35 40 45

Lys Val Thr Asp Lys Met Asp Ala Ala Gly Leu Ala Trp Ala Ile Tyr

50 55 60

Asp Gly Val Val Pro Asn Pro Thr Ile Thr Val Val Lys Glu Gly Leu

65 70 75 80

Gly Val Phe Gln Asn Ser Gly Ala Asp Tyr Leu Ile Ala Ile Gly Gly

85 90 95

Gly Ser Pro Gln Asp Thr Cys Lys Ala Ile Gly Ile Ile Ser Asn Asn

100 105 110

Pro Glu Phe Ala Asp Val Arg Ser Leu Glu Gly Leu Ser Pro Thr Asn

115 120 125

Lys Pro Ser Val Pro Ile Leu Ala Ile Pro Thr Thr Ala Gly Thr Ala

130 135 140

Ala Glu Val Thr Ile Asn Tyr Val Ile Thr Asp Glu Glu Lys Arg Arg

145 150 155 160

Lys Phe Val Cys Val Asp Pro His Asp Ile Pro Gln Val Ala Phe Ile

165 170 175

Asp Ala Asp Met Met Asp Gly Met Pro Pro Ala Leu Lys Ala Ala Thr

180 185 190

Gly Val Asp Ala Leu Thr His Ala Ile Glu Gly Tyr Ile Thr Arg Gly

195 200 205

Ala Trp Ala Leu Thr Asp Ala Leu His Ile Lys Ala Ile Glu Ile Ile

210 215 220

Ala Gly Ala Leu Arg Gly Ser Val Ala Gly Asp Lys Asp Ala Gly Glu

225 230 235 240

Glu Met Ala Leu Gly Gln Tyr Val Ala Gly Met Gly Phe Ser Asn Val

245 250 255

Gly Leu Gly Leu Val His Gly Met Ala His Pro Leu Gly Ala Phe Tyr

260 265 270

Asn Thr Pro His Gly Val Ala Asn Ala Ile Leu Leu Pro His Val Met

275 280 285

Arg Tyr Asn Ala Asp Phe Thr Gly Glu Lys Tyr Arg Asp Ile Ala Arg

290 295 300

Val Met Gly Val Lys Val Glu Gly Met Ser Leu Glu Glu Ala Arg Asn

305 310 315 320

Ala Ala Val Glu Ala Val Phe Ala Leu Asn Arg Asp Val Gly Ile Pro

325 330 335

Pro His Leu Arg Asp Val Gly Val Arg Lys Glu Asp Ile Pro Ala Leu

340 345 350

Ala Gln Ala Ala Leu Asp Asp Val Cys Thr Gly Gly Asn Pro Arg Glu

355 360 365

Ala Thr Leu Glu Asp Ile Val Glu Leu Tyr His Thr Ala Trp

370 375 380

<210> 42

<211> 312

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 42

Met Asp Ile Ile Phe Tyr His Pro Thr Phe Asp Thr Gln Trp Trp Ile

1 5 10 15

Glu Ala Leu Arg Lys Ala Ile Pro Gln Ala Arg Val Arg Ala Trp Lys

20 25 30

Ser Gly Asp Asn Asp Ser Ala Asp Tyr Ala Leu Val Trp His Pro Pro

35 40 45

Val Glu Met Leu Ala Gly Arg Asp Leu Lys Ala Val Phe Ala Leu Gly

50 55 60

Ala Gly Val Asp Ser Ile Leu Ser Lys Leu Gln Ala His Pro Glu Met

65 70 75 80

Leu Asn Pro Ser Val Pro Leu Phe Arg Leu Glu Asp Thr Gly Met Gly

85 90 95

Glu Gln Met Gln Glu Tyr Ala Val Ser Gln Val Leu His Trp Phe Arg

100 105 110

Arg Phe Asp Asp Tyr Arg Ile Gln Gln Asn Ser Ser His Trp Gln Pro

115 120 125

Leu Pro Glu Tyr His Arg Glu Asp Phe Thr Ile Gly Ile Leu Gly Ala

130 135 140

Gly Val Leu Gly Ser Lys Val Ala Gln Ser Leu Gln Thr Trp Arg Phe

145 150 155 160

Pro Leu Arg Cys Trp Ser Arg Thr Arg Lys Ser Trp Pro Gly Val Gln

165 170 175

Ser Phe Ala Gly Arg Glu Glu Leu Ser Ala Phe Leu Ser Gln Cys Arg

180 185 190

Val Leu Ile Asn Leu Leu Pro Asn Thr Pro Glu Thr Val Gly Ile Ile

195 200 205

Asn Gln Gln Leu Leu Glu Lys Leu Pro Asp Gly Ala Tyr Leu Leu Asn

210 215 220

Leu Ala Arg Gly Val His Val Val Glu Asp Asp Leu Leu Ala Ala Leu

225 230 235 240

Asp Ser Gly Lys Val Lys Gly Ala Met Leu Asp Val Phe Asn Arg Glu

245 250 255

Pro Leu Pro Pro Glu Ser Pro Leu Trp Gln His Pro Arg Val Thr Ile

260 265 270

Thr Pro His Val Ala Ala Ile Thr Arg Pro Ala Glu Ala Val Glu Tyr

275 280 285

Ile Ser Arg Thr Ile Ala Gln Leu Glu Lys Gly Glu Arg Val Cys Gly

290 295 300

Gln Val Asp Arg Ala Arg Gly Tyr

305 310

<210> 43

<211> 324

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 43

Met Lys Pro Ser Val Ile Leu Tyr Lys Ala Leu Pro Asp Asp Leu Leu

1 5 10 15

Gln Arg Leu Gln Glu His Phe Thr Val His Gln Val Ala Asn Leu Ser

20 25 30

Pro Gln Thr Val Glu Gln Asn Ala Ala Ile Phe Ala Glu Ala Glu Gly

35 40 45

Leu Leu Gly Ser Asn Glu Asn Val Asn Ala Ala Leu Leu Glu Lys Met

50 55 60

Pro Lys Leu Arg Ala Thr Ser Thr Ile Ser Val Gly Tyr Asp Asn Phe

65 70 75 80

Asp Val Asp Ala Leu Thr Ala Arg Lys Ile Leu Leu Met His Thr Pro

85 90 95

Thr Val Leu Thr Glu Thr Val Ala Asp Thr Leu Met Ala Leu Val Leu

100 105 110

Ser Thr Ala Arg Arg Val Val Glu Val Ala Glu Arg Val Lys Ala Gly

115 120 125

Glu Trp Thr Ala Ser Ile Gly Pro Asp Trp Tyr Gly Thr Asp Val His

130 135 140

His Lys Thr Leu Gly Ile Val Gly Met Gly Arg Ile Gly Met Ala Leu

145 150 155 160

Ala Gln Arg Ala His Phe Gly Phe Asn Met Pro Ile Leu Tyr Asn Ala

165 170 175

Arg Arg His His Lys Glu Ala Glu Glu Arg Phe Asn Ala Arg Tyr Cys

180 185 190

Asp Leu Asp Thr Leu Leu Gln Glu Ser Asp Phe Val Cys Leu Ile Leu

195 200 205

Pro Leu Thr Asp Glu Thr His His Leu Phe Gly Ala Glu Gln Phe Ala

210 215 220

Lys Met Lys Ser Ser Ala Ile Phe Ile Asn Ala Gly Arg Gly Pro Val

225 230 235 240

Val Asp Glu Asn Ala Leu Ile Ala Ala Leu Gln Lys Gly Glu Ile His

245 250 255

Ala Ala Gly Leu Asp Val Phe Glu Gln Glu Pro Leu Ser Val Asp Ser

260 265 270

Pro Leu Leu Ser Met Ala Asn Val Val Ala Val Pro His Ile Gly Ser

275 280 285

Ala Thr His Glu Thr Arg Tyr Gly Met Ala Ala Cys Ala Val Asp Asn

290 295 300

Leu Ile Asp Ala Leu Gln Gly Lys Val Glu Lys Asn Cys Val Asn Pro

305 310 315 320

His Val Ala Asp

<210> 44

<211> 275

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 44

Met Ala Asn Pro Thr Val Ile Lys Leu Gln Asp Gly Asn Val Met Pro

1 5 10 15

Gln Leu Gly Leu Gly Val Trp Gln Ala Ser Asn Glu Glu Val Ile Thr

20 25 30

Ala Ile Gln Lys Ala Leu Glu Val Gly Tyr Arg Ser Ile Asp Thr Ala

35 40 45

Ala Ala Tyr Lys Asn Glu Glu Gly Val Gly Lys Ala Leu Lys Asn Ala

50 55 60

Ser Val Asn Arg Glu Glu Leu Phe Ile Thr Thr Lys Leu Trp Asn Asp

65 70 75 80

Asp His Lys Arg Pro Arg Glu Ala Leu Leu Asp Ser Leu Lys Lys Leu

85 90 95

Gln Leu Asp Tyr Ile Asp Leu Tyr Leu Met His Trp Pro Val Pro Ala

100 105 110

Ile Asp His Tyr Val Glu Ala Trp Lys Gly Met Ile Glu Leu Gln Lys

115 120 125

Glu Gly Leu Ile Lys Ser Ile Gly Val Cys Asn Phe Gln Ile His His

130 135 140

Leu Gln Arg Leu Ile Asp Glu Thr Gly Val Thr Pro Val Ile Asn Gln

145 150 155 160

Ile Glu Leu His Pro Leu Met Gln Gln Arg Gln Leu His Ala Trp Asn

165 170 175

Ala Thr His Lys Ile Gln Thr Glu Ser Trp Ser Pro Leu Ala Gln Gly

180 185 190

Gly Lys Gly Val Phe Asp Gln Lys Val Ile Arg Asp Leu Ala Asp Lys

195 200 205

Tyr Gly Lys Thr Pro Ala Gln Ile Val Ile Arg Trp His Leu Asp Ser

210 215 220

Gly Leu Val Val Ile Pro Lys Ser Val Thr Pro Ser Arg Ile Ala Glu

225 230 235 240

Asn Phe Asp Val Trp Asp Phe Arg Leu Asp Lys Asp Glu Leu Gly Glu

245 250 255

Ile Ala Lys Leu Asp Gln Gly Lys Arg Leu Gly Pro Asp Pro Asp Gln

260 265 270

Phe Gly Gly

275

<210> 45

<211> 267

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 45

Met Ala Ile Pro Ala Phe Gly Leu Gly Thr Phe Arg Leu Lys Asp Asp

1 5 10 15

Val Val Ile Ser Ser Val Ile Thr Ala Leu Glu Leu Gly Tyr Arg Ala

20 25 30

Ile Asp Thr Ala Gln Ile Tyr Asp Asn Glu Ala Ala Val Gly Gln Ala

35 40 45

Ile Ala Glu Ser Gly Val Pro Arg His Glu Leu Tyr Ile Thr Thr Lys

50 55 60

Ile Trp Ile Glu Asn Leu Ser Lys Asp Lys Leu Ile Pro Ser Leu Lys

65 70 75 80

Glu Ser Leu Gln Lys Leu Arg Thr Asp Tyr Val Asp Leu Thr Leu Ile

85 90 95

His Trp Pro Ser Pro Asn Asp Glu Val Ser Val Glu Glu Phe Met Gln

100 105 110

Ala Leu Leu Glu Ala Lys Lys Gln Gly Leu Thr Arg Glu Ile Gly Ile

115 120 125

Ser Asn Phe Thr Ile Pro Leu Met Glu Lys Ala Ile Ala Ala Val Gly

130 135 140

Ala Glu Asn Ile Ala Thr Asn Gln Ile Glu Leu Ser Pro Tyr Leu Gln

145 150 155 160

Asn Arg Lys Val Val Ala Trp Ala Lys Gln His Gly Ile His Ile Thr

165 170 175

Ser Tyr Met Thr Leu Ala Tyr Gly Lys Ala Leu Lys Asp Glu Val Ile

180 185 190

Ala Arg Ile Ala Ala Lys His Asn Ala Thr Pro Ala Gln Val Ile Leu

195 200 205

Ala Trp Ala Met Gly Glu Gly Tyr Ser Val Ile Pro Ser Ser Thr Lys

210 215 220

Arg Lys Asn Leu Glu Ser Asn Leu Lys Ala Gln Asn Leu Gln Leu Asp

225 230 235 240

Ala Glu Asp Lys Lys Ala Ile Ala Ala Leu Asp Cys Asn Asp Arg Leu

245 250 255

Val Ser Pro Glu Gly Leu Ala Pro Glu Trp Asp

260 265

<210> 46

<211> 495

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 46

Met Asn Phe His His Leu Ala Tyr Trp Gln Asp Lys Ala Leu Ser Leu

1 5 10 15

Ala Ile Glu Asn Arg Leu Phe Ile Asn Gly Glu Tyr Thr Ala Ala Ala

20 25 30

Glu Asn Glu Thr Phe Glu Thr Val Asp Pro Val Thr Gln Ala Pro Leu

35 40 45

Ala Lys Ile Ala Arg Gly Lys Ser Val Asp Ile Asp Arg Ala Met Ser

50 55 60

Ala Ala Arg Gly Val Phe Glu Arg Gly Asp Trp Ser Leu Ser Ser Pro

65 70 75 80

Ala Lys Arg Lys Ala Val Leu Asn Lys Leu Ala Asp Leu Met Glu Ala

85 90 95

His Ala Glu Glu Leu Ala Leu Leu Glu Thr Leu Asp Thr Gly Lys Pro

100 105 110

Ile Arg His Ser Leu Arg Asp Asp Ile Pro Gly Ala Ala Arg Ala Ile

115 120 125

Arg Trp Tyr Ala Glu Ala Ile Asp Lys Val Tyr Gly Glu Val Ala Thr

130 135 140

Thr Ser Ser His Glu Leu Ala Met Ile Val Arg Glu Pro Val Gly Val

145 150 155 160

Ile Ala Ala Ile Val Pro Trp Asn Phe Pro Leu Leu Leu Thr Cys Trp

165 170 175

Lys Leu Gly Pro Ala Leu Ala Ala Gly Asn Ser Val Ile Leu Lys Pro

180 185 190

Ser Glu Lys Ser Pro Leu Ser Ala Ile Arg Leu Ala Gly Leu Ala Lys

195 200 205

Glu Ala Gly Leu Pro Asp Gly Val Leu Asn Val Val Thr Gly Phe Gly

210 215 220

His Glu Ala Gly Gln Ala Leu Ser Arg His Asn Asp Ile Asp Ala Ile

225 230 235 240

Ala Phe Thr Gly Ser Thr Arg Thr Gly Lys Gln Leu Leu Lys Asp Ala

245 250 255

Gly Asp Ser Asn Met Lys Arg Val Trp Leu Glu Ala Gly Gly Lys Ser

260 265 270

Ala Asn Ile Val Phe Ala Asp Cys Pro Asp Leu Gln Gln Ala Ala Ser

275 280 285

Ala Thr Ala Ala Gly Ile Phe Tyr Asn Gln Gly Gln Val Cys Ile Ala

290 295 300

Gly Thr Arg Leu Leu Leu Glu Glu Ser Ile Ala Asp Glu Phe Leu Ala

305 310 315 320

Leu Leu Lys Gln Gln Ala Gln Asn Trp Gln Pro Gly His Pro Leu Asp

325 330 335

Pro Ala Thr Thr Met Gly Thr Leu Ile Asp Cys Ala His Ala Asp Ser

340 345 350

Val His Ser Phe Ile Arg Glu Gly Glu Ser Lys Gly Gln Leu Leu Leu

355 360 365

Asp Gly Arg Asn Ala Gly Leu Ala Ala Ala Ile Gly Pro Thr Ile Phe

370 375 380

Val Asp Val Asp Pro Asn Ala Ser Leu Ser Arg Glu Glu Ile Phe Gly

385 390 395 400

Pro Val Leu Val Val Thr Arg Phe Thr Ser Glu Glu Gln Ala Leu Gln

405 410 415

Leu Ala Asn Asp Ser Gln Tyr Gly Leu Gly Ala Ala Val Trp Thr Arg

420 425 430

Asp Leu Ser Arg Ala His Arg Met Ser Arg Arg Leu Lys Ala Gly Ser

435 440 445

Val Phe Val Asn Asn Tyr Asn Asp Gly Asp Met Thr Val Pro Phe Gly

450 455 460

Gly Tyr Lys Gln Ser Gly Asn Gly Arg Asp Lys Ser Leu His Ala Leu

465 470 475 480

Glu Lys Phe Thr Glu Leu Lys Thr Ile Trp Ile Ser Leu Glu Ala

485 490 495

<210> 47

<211> 496

<212> PRT

<213>Friedlander's bacillus (Klebsiella pneumoniae)

<400> 47

Met Met Asn Phe Gln His Leu Ala Tyr Trp Gln Glu Lys Ala Lys Asn

1 5 10 15

Leu Ala Ile Glu Thr Arg Leu Phe Ile Asn Gly Glu Tyr Cys Ala Ala

20 25 30

Ala Asp Asn Thr Thr Phe Glu Thr Ile Asp Pro Ala Ala Gln Gln Thr

35 40 45

Leu Ala Gln Val Ala Arg Gly Lys Lys Ala Asp Val Glu Arg Ala Val

50 55 60

Lys Ala Ala Arg Gln Ala Phe Asp Asn Gly Asp Trp Ser Gln Ala Ser

65 70 75 80

Pro Ala Gln Arg Lys Ala Ile Leu Thr Arg Phe Ala Asn Leu Met Glu

85 90 95

Ala His Arg Glu Glu Leu Ala Leu Leu Glu Thr Leu Asp Thr Gly Lys

100 105 110

Pro Ile Arg His Ser Leu Arg Asp Asp Ile Pro Gly Ala Ala Arg Ala

115 120 125

Ile Arg Trp Tyr Ala Glu Ala Leu Asp Lys Val Tyr Gly Glu Val Ala

130 135 140

Pro Thr Gly Ser Asn Glu Leu Ala Met Ile Val Arg Glu Pro Ile Gly

145 150 155 160

Val Ile Ala Ala Val Val Pro Trp Asn Phe Pro Leu Leu Leu Ala Cys

165 170 175

Trp Lys Leu Gly Pro Ala Leu Ala Ala Gly Asn Ser Val Ile Leu Lys

180 185 190

Pro Ser Glu Lys Ser Pro Leu Thr Ala Leu Arg Leu Ala Gly Leu Ala

195 200 205

Lys Glu Ala Gly Leu Pro Asp Gly Val Leu Asn Val Val Ser Gly Phe

210 215 220

Gly His Glu Ala Gly Gln Ala Leu Ala Leu His Pro Asp Val Glu Val

225 230 235 240

Ile Thr Phe Thr Gly Ser Thr Arg Thr Gly Lys Gln Leu Leu Lys Asp

245 250 255

Ala Gly Asp Ser Asn Met Lys Arg Val Trp Leu Glu Ala Gly Gly Lys

260 265 270

Ser Ala Asn Ile Val Phe Ala Asp Cys Pro Asp Leu Gln Gln Ala Val

275 280 285

Arg Ala Thr Ala Gly Gly Ile Phe Tyr Asn Gln Gly Gln Val Cys Ile

290 295 300

Ala Gly Thr Arg Leu Leu Leu Glu Glu Ser Ile Ala Asp Glu Phe Leu

305 310 315 320

Ala Arg Leu Lys Ala Glu Ala Gln His Trp Gln Pro Gly Asn Pro Leu

325 330 335

Asp Pro Asp Thr Thr Met Gly Met Leu Ile Asp Asn Thr His Ala Asp

340 345 350

Asn Val His Ser Phe Ile Arg Gly Gly Glu Ser Gln Ser Thr Leu Phe

355 360 365

Leu Asp Gly Arg Lys Asn Pro Trp Pro Ala Ala Val Gly Pro Thr Ile

370 375 380

Phe Val Asp Val Asp Pro Ala Ser Thr Leu Ser Arg Glu Glu Ile Phe

385 390 395 400

Gly Pro Val Leu Val Val Thr Arg Phe Lys Ser Glu Glu Glu Ala Leu

405 410 415

Lys Leu Ala Asn Asp Ser Asp Tyr Gly Leu Gly Ala Ala Val Trp Thr

420 425 430

Arg Asp Leu Ser Arg Ala His Arg Met Ser Arg Arg Leu Lys Ala Gly

435 440 445

Ser Val Phe Val Asn Asn Tyr Asn Asp Gly Asp Met Thr Val Pro Phe

450 455 460

Gly Gly Tyr Lys Gln Ser Gly Asn Gly Arg Asp Lys Ser Leu His Ala

465 470 475 480

Leu Glu Lys Phe Thr Glu Leu Lys Thr Ile Trp Ile Ala Leu Glu Ser

485 490 495

<210> 48

<211> 519

<212> PRT

<213>Saccharomyces cerevisiae (Saccharomyces cerevisiae)

<400> 48

Met Phe Ser Arg Ser Thr Leu Cys Leu Lys Thr Ser Ala Ser Ser Ile

1 5 10 15

Gly Arg Leu Gln Leu Arg Tyr Phe Ser His Leu Pro Met Thr Val Pro

20 25 30

Ile Lys Leu Pro Asn Gly Leu Glu Tyr Glu Gln Pro Thr Gly Leu Phe

35 40 45

Ile Asn Asn Lys Phe Val Pro Ser Lys Gln Asn Lys Thr Phe Glu Val

50 55 60

Ile Asn Pro Ser Thr Glu Glu Glu Ile Cys His Ile Tyr Glu Gly Arg

65 70 75 80

Glu Asp Asp Val Glu Glu Ala Val Gln Ala Ala Asp Arg Ala Phe Ser

85 90 95

Asn Gly Ser Trp Asn Gly Ile Asp Pro Ile Asp Arg Gly Lys Ala Leu

100 105 110

Tyr Arg Leu Ala Glu Leu Ile Glu Gln Asp Lys Asp Val Ile Ala Ser

115 120 125

Ile Glu Thr Leu Asp Asn Gly Lys Ala Ile Ser Ser Ser Arg Gly Asp

130 135 140

Val Asp Leu Val Ile Asn Tyr Leu Lys Ser Ser Ala Gly Phe Ala Asp

145 150 155 160

Lys Ile Asp Gly Arg Met Ile Asp Thr Gly Arg Thr His Phe Ser Tyr

165 170 175

Thr Lys Arg Gln Pro Leu Gly Val Cys Gly Gln Ile Ile Pro Trp Asn

180 185 190

Phe Pro Leu Leu Met Trp Ala Trp Lys Ile Ala Pro Ala Leu Val Thr

195 200 205

Gly Asn Thr Val Val Leu Lys Thr Ala Glu Ser Thr Pro Leu Ser Ala

210 215 220

Leu Tyr Val Ser Lys Tyr Ile Pro Gln Ala Gly Ile Pro Pro Gly Val

225 230 235 240

Ile Asn Ile Val Ser Gly Phe Gly Lys Ile Val Gly Glu Ala Ile Thr

245 250 255

Asn His Pro Lys Ile Lys Lys Val Ala Phe Thr Gly Ser Thr Ala Thr

260 265 270

Gly Arg His Ile Tyr Gln Ser Ala Ala Ala Gly Leu Lys Lys Val Thr

275 280 285

Leu Glu Leu Gly Gly Lys Ser Pro Asn Ile Val Phe Ala Asp Ala Glu

290 295 300

Leu Lys Lys Ala Val Gln Asn Ile Ile Leu Gly Ile Tyr Tyr Asn Ser

305 310 315 320

Gly Glu Val Cys Cys Ala Gly Ser Arg Val Tyr Val Glu Glu Ser Ile

325 330 335

Tyr Asp Lys Phe Ile Glu Glu Phe Lys Ala Ala Ser Glu Ser Ile Lys

340 345 350

Val Gly Asp Pro Phe Asp Glu Ser Thr Phe Gln Gly Ala Gln Thr Ser

355 360 365

Gln Met Gln Leu Asn Lys Ile Leu Lys Tyr Val Asp Ile Gly Lys Asn

370 375 380

Glu Gly Ala Thr Leu Ile Thr Gly Gly Glu Arg Leu Gly Ser Lys Gly

385 390 395 400

Tyr Phe Ile Lys Pro Thr Val Phe Gly Asp Val Lys Glu Asp Met Arg

405 410 415

Ile Val Lys Glu Glu Ile Phe Gly Pro Val Val Thr Val Thr Lys Phe

420 425 430

Lys Ser Ala Asp Glu Val Ile Asn Met Ala Asn Asp Ser Glu Tyr Gly

435 440 445

Leu Ala Ala Gly Ile His Thr Ser Asn Ile Asn Thr Ala Leu Lys Val

450 455 460

Ala Asp Arg Val Asn Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asp

465 470 475 480

Phe His His Ala Val Pro Phe Gly Gly Phe Asn Ala Ser Gly Leu Gly

485 490 495

Arg Glu Met Ser Val Asp Ala Leu Gln Asn Tyr Leu Gln Val Lys Ala

500 505 510

Val Arg Ala Lys Leu Asp Glu

515

<210> 49

<211> 520

<212> PRT

<213>Saccharomyces cerevisiae (Saccharomyces cerevisiae)

<400> 49

Met Leu Ser Arg Thr Arg Ala Ala Ala Pro Asn Ser Arg Ile Phe Thr

1 5 10 15

Arg Ser Leu Leu Arg Leu Tyr Ser Gln Ala Pro Leu Arg Val Pro Ile

20 25 30

Thr Leu Pro Asn Gly Phe Thr Tyr Glu Gln Pro Thr Gly Leu Phe Ile

35 40 45

Asn Gly Glu Phe Val Ala Ser Lys Gln Lys Lys Thr Phe Asp Val Ile

50 55 60

Asn Pro Ser Asn Glu Glu Lys Ile Thr Thr Val Tyr Lys Ala Met Glu

65 70 75 80

Asp Asp Val Asp Glu Ala Val Ala Ala Ala Lys Lys Ala Phe Glu Thr

85 90 95

Lys Trp Ser Ile Val Glu Pro Glu Val Arg Ala Lys Ala Leu Phe Asn

100 105 110

Leu Ala Asp Leu Val Glu Lys His Gln Glu Thr Leu Ala Ala Ile Glu

115 120 125

Ser Met Asp Asn Gly Lys Ser Leu Phe Cys Ala Arg Gly Asp Val Ala

130 135 140

Leu Val Ser Lys Tyr Leu Arg Ser Cys Gly Gly Trp Ala Asp Lys Ile

145 150 155 160

Tyr Gly Asn Val Ile Asp Thr Gly Lys Asn His Phe Thr Tyr Ser Ile

165 170 175

Lys Glu Pro Leu Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro

180 185 190

Leu Leu Met Trp Ser Trp Lys Ile Gly Pro Ala Leu Ala Thr Gly Asn

195 200 205

Thr Val Val Leu Lys Pro Ala Glu Thr Thr Pro Leu Ser Ala Leu Phe

210 215 220

Ala Ser Gln Leu Cys Gln Glu Ala Gly Ile Pro Ala Gly Val Val Asn

225 230 235 240

Ile Leu Pro Gly Ser Gly Arg Val Val Gly Glu Arg Leu Ser Ala His

245 250 255

Pro Asp Val Lys Lys Ile Ala Phe Thr Gly Ser Thr Ala Thr Gly Arg

260 265 270

His Ile Met Lys Val Ala Ala Asp Thr Val Lys Lys Val Thr Leu Glu

275 280 285

Leu Gly Gly Lys Ser Pro Asn Ile Val Phe Ala Asp Ala Asp Leu Asp

290 295 300

Lys Ala Val Lys Asn Ile Ala Phe Gly Ile Phe Tyr Asn Ser Gly Glu

305 310 315 320

Val Cys Cys Ala Gly Ser Arg Ile Tyr Ile Gln Asp Thr Val Tyr Glu

325 330 335

Glu Val Leu Gln Lys Leu Lys Asp Tyr Thr Glu Ser Leu Lys Val Gly

340 345 350

Asp Pro Phe Asp Glu Glu Val Phe Gln Gly Ala Gln Thr Ser Asp Lys

355 360 365

Gln Leu His Lys Ile Leu Asp Tyr Val Asp Val Ala Lys Ser Glu Gly

370 375 380

Ala Arg Leu Val Thr Gly Gly Ala Arg His Gly Ser Lys Gly Tyr Phe

385 390 395 400

Val Lys Pro Thr Val Phe Ala Asp Val Lys Glu Asp Met Arg Ile Val

405 410 415

Lys Glu Glu Val Phe Gly Pro Ile Val Thr Val Ser Lys Phe Ser Thr

420 425 430

Val Asp Glu Val Ile Ala Met Ala Asn Asp Ser Gln Tyr Gly Leu Ala

435 440 445

Ala Gly Ile His Thr Asn Asp Ile Asn Lys Ala Val Asp Val Ser Lys

450 455 460

Arg Val Lys Ala Gly Thr Val Trp Ile Asn Thr Tyr Asn Asn Phe His

465 470 475 480

Gln Asn Val Pro Phe Gly Gly Phe Gly Gln Ser Gly Ile Gly Arg Glu

485 490 495

Met Gly Glu Ala Ala Leu Ser Asn Tyr Thr Gln Thr Lys Ser Val Arg

500 505 510

Ile Ala Ile Asp Lys Pro Ile Arg

515 520

<210> 50

<211> 462

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 50

Met Thr Ile Thr Pro Ala Thr His Ala Ile Ser Ile Asn Pro Ala Thr

1 5 10 15

Gly Glu Gln Leu Ser Val Leu Pro Trp Ala Gly Ala Asp Asp Ile Glu

20 25 30

Asn Ala Leu Gln Leu Ala Ala Ala Gly Phe Arg Asp Trp Arg Glu Thr

35 40 45

Asn Ile Asp Tyr Arg Ala Glu Lys Leu Arg Asp Ile Gly Lys Ala Leu

50 55 60

Arg Ala Arg Ser Glu Glu Met Ala Gln Met Ile Thr Arg Glu Met Gly

65 70 75 80

Lys Pro Ile Asn Gln Ala Arg Ala Glu Val Ala Lys Ser Ala Asn Leu

85 90 95

Cys Asp Trp Tyr Ala Glu His Gly Pro Ala Met Leu Lys Ala Glu Pro

100 105 110

Thr Leu Val Glu Asn Gln Gln Ala Val Ile Glu Tyr Arg Pro Leu Gly

115 120 125

Thr Ile Leu Ala Ile Met Pro Trp Asn Phe Pro Leu Trp Gln Val Met

130 135 140

Arg Gly Ala Val Pro Ile Ile Leu Ala Gly Asn Gly Tyr Leu Leu Lys

145 150 155 160

His Ala Pro Asn Val Met Gly Cys Ala Gln Leu Ile Ala Gln Val Phe

165 170 175

Lys Asp Ala Gly Ile Pro Gln Gly Val Tyr Gly Trp Leu Asn Ala Asp

180 185 190

Asn Asp Gly Val Ser Gln Met Ile Lys Asp Ser Arg Ile Ala Ala Val

195 200 205

Thr Val Thr Gly Ser Val Arg Ala Gly Ala Ala Ile Gly Ala Gln Ala

210 215 220

Gly Ala Ala Leu Lys Lys Cys Val Leu Glu Leu Gly Gly Ser Asp Pro

225 230 235 240

Phe Ile Val Leu Asn Asp Ala Asp Leu Glu Leu Ala Val Lys Ala Ala

245 250 255

Val Ala Gly Arg Tyr Gln Asn Thr Gly Gln Val Cys Ala Ala Ala Lys

260 265 270

Arg Phe Ile Ile Glu Glu Gly Ile Ala Ser Ala Phe Thr Glu Arg Phe

275 280 285

Val Ala Ala Ala Ala Ala Leu Lys Met Gly Asp Pro Arg Asp Glu Glu

290 295 300

Asn Ala Leu Gly Pro Met Ala Arg Phe Asp Leu Arg Asp Glu Leu His

305 310 315 320

His Gln Val Glu Lys Thr Leu Ala Gln Gly Ala Arg Leu Leu Leu Gly

325 330 335

Gly Glu Lys Met Ala Gly Ala Gly Asn Tyr Tyr Pro Pro Thr Val Leu

340 345 350

Ala Asn Val Thr Pro Glu Met Thr Ala Phe Arg Glu Glu Met Phe Gly

355 360 365

Pro Val Ala Ala Ile Thr Ile Ala Lys Asp Ala Glu His Ala Leu Glu

370 375 380

Leu Ala Asn Asp Ser Glu Phe Gly Leu Ser Ala Thr Ile Phe Thr Thr

385 390 395 400

Asp Glu Thr Gln Ala Arg Gln Met Ala Ala Arg Leu Glu Cys Gly Gly

405 410 415

Val Phe Ile Asn Gly Tyr Cys Ala Ser Asp Ala Arg Val Ala Phe Gly

420 425 430

Gly Val Lys Lys Ser Gly Phe Gly Arg Glu Leu Ser His Phe Gly Leu

435 440 445

His Glu Phe Cys Asn Ile Gln Thr Val Trp Lys Asp Arg Ile

450 455 460

<210> 51

<211> 482

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 51

Met Lys Leu Asn Asp Ser Asn Leu Phe Arg Gln Gln Ala Leu Ile Asn

1 5 10 15

Gly Glu Trp Leu Asp Ala Asn Asn Gly Glu Ala Ile Asp Val Thr Asn

20 25 30

Pro Ala Asn Gly Asp Lys Leu Gly Ser Val Pro Lys Met Gly Ala Asp

35 40 45

Glu Thr Arg Ala Ala Ile Asp Ala Ala Asn Arg Ala Leu Pro Ala Trp

50 55 60

Arg Ala Leu Thr Ala Lys Glu Arg Ala Thr Ile Leu Arg Asn Trp Phe

65 70 75 80

Asn Leu Met Met Glu His Gln Asp Asp Leu Ala Arg Leu Met Thr Leu

85 90 95

Glu Gln Gly Lys Pro Leu Ala Glu Ala Lys Gly Glu Ile Ser Tyr Ala

100 105 110

Ala Ser Phe Ile Glu Trp Phe Ala Glu Glu Gly Lys Arg Ile Tyr Gly

115 120 125

Asp Thr Ile Pro Gly His Gln Ala Asp Lys Arg Leu Ile Val Ile Lys

130 135 140

Gln Pro Ile Gly Val Thr Ala Ala Ile Thr Pro Trp Asn Phe Pro Ala

145 150 155 160

Ala Met Ile Thr Arg Lys Ala Gly Pro Ala Leu Ala Ala Gly Cys Thr

165 170 175

Met Val Leu Lys Pro Ala Ser Gln Thr Pro Phe Ser Ala Leu Ala Leu

180 185 190

Ala Glu Leu Ala Ile Arg Ala Gly Val Pro Ala Gly Val Phe Asn Val

195 200 205

Val Thr Gly Ser Ala Gly Ala Val Gly Asn Glu Leu Thr Ser Asn Pro

210 215 220

Leu Val Arg Lys Leu Ser Phe Thr Gly Ser Thr Glu Ile Gly Arg Gln

225 230 235 240

Leu Met Glu Gln Cys Ala Lys Asp Ile Lys Lys Val Ser Leu Glu Leu

245 250 255

Gly Gly Asn Ala Pro Phe Ile Val Phe Asp Asp Ala Asp Leu Asp Lys

260 265 270

Ala Val Glu Gly Ala Leu Ala Ser Lys Phe Arg Asn Ala Gly Gln Thr

275 280 285

Cys Val Cys Ala Asn Arg Leu Tyr Val Gln Asp Gly Val Tyr Asp Arg

290 295 300

Phe Ala Glu Lys Leu Gln Gln Ala Val Ser Lys Leu His Ile Gly Asp

305 310 315 320

Gly Leu Asp Asn Gly Val Thr Ile Gly Pro Leu Ile Asp Glu Lys Ala

325 330 335

Val Ala Lys Val Glu Glu His Ile Ala Asp Ala Leu Glu Lys Gly Ala

340 345 350

Arg Val Val Cys Gly Gly Lys Ala His Glu Arg Gly Gly Asn Phe Phe

355 360 365

Gln Pro Thr Ile Leu Val Asp Val Pro Ala Asn Ala Lys Val Ser Lys

370 375 380

Glu Glu Thr Phe Gly Pro Leu Ala Pro Leu Phe Arg Phe Lys Asp Glu

385 390 395 400

Ala Asp Val Ile Ala Gln Ala Asn Asp Thr Glu Phe Gly Leu Ala Ala

405 410 415

Tyr Phe Tyr Ala Arg Asp Leu Ser Arg Val Phe Arg Val Gly Glu Ala

420 425 430

Leu Glu Tyr Gly Ile Val Gly Ile Asn Thr Gly Ile Ile Ser Asn Glu

435 440 445

Val Ala Pro Phe Gly Gly Ile Lys Ala Ser Gly Leu Gly Arg Glu Gly

450 455 460

Ser Lys Tyr Gly Ile Glu Asp Tyr Leu Glu Ile Lys Tyr Met Cys Ile

465 470 475 480

Gly Leu

<210> 52

<211> 474

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 52

Met Gln His Lys Leu Leu Ile Asn Gly Glu Leu Val Ser Gly Glu Gly

1 5 10 15

Glu Lys Gln Pro Val Tyr Asn Pro Ala Thr Gly Asp Val Leu Leu Glu

20 25 30

Ile Ala Glu Ala Ser Ala Glu Gln Val Asp Ala Ala Val Arg Ala Ala

35 40 45

Asp Ala Ala Phe Ala Glu Trp Gly Gln Thr Thr Pro Lys Val Arg Ala

50 55 60

Glu Cys Leu Leu Lys Leu Ala Asp Val Ile Glu Glu Asn Gly Gln Val

65 70 75 80

Phe Ala Glu Leu Glu Ser Arg Asn Cys Gly Lys Pro Leu His Ser Ala

85 90 95

Phe Asn Asp Glu Ile Pro Ala Ile Val Asp Val Phe Arg Phe Phe Ala

100 105 110

Gly Ala Ala Arg Cys Leu Asn Gly Leu Ala Ala Gly Glu Tyr Leu Glu

115 120 125

Gly His Thr Ser Met Ile Arg Arg Asp Pro Leu Gly Val Val Ala Ser

130 135 140

Ile Ala Pro Trp Asn Tyr Pro Leu Met Met Ala Ala Trp Lys Leu Ala

145 150 155 160

Pro Ala Leu Ala Ala Gly Asn Cys Val Val Leu Lys Pro Ser Glu Ile

165 170 175

Thr Pro Leu Thr Ala Leu Lys Leu Ala Glu Leu Ala Lys Asp Ile Phe

180 185 190

Pro Ala Gly Val Ile Asn Ile Leu Phe Gly Arg Gly Lys Thr Val Gly

195 200 205

Asp Pro Leu Thr Gly His Pro Lys Val Arg Met Val Ser Leu Thr Gly

210 215 220

Ser Ile Ala Thr Gly Glu His Ile Ile Ser His Thr Ala Ser Ser Ile

225 230 235 240

Lys Arg Thr His Met Glu Leu Gly Gly Lys Ala Pro Val Ile Val Phe

245 250 255

Asp Asp Ala Asp Ile Glu Ala Val Val Glu Gly Val Arg Thr Phe Gly

260 265 270

Tyr Tyr Asn Ala Gly Gln Asp Cys Thr Ala Ala Cys Arg Ile Tyr Ala

275 280 285

Gln Lys Gly Ile Tyr Asp Thr Leu Val Glu Lys Leu Gly Ala Ala Val

290 295 300

Ala Thr Leu Lys Ser Gly Ala Pro Asp Asp Glu Ser Thr Glu Leu Gly

305 310 315 320

Pro Leu Ser Ser Leu Ala His Leu Glu Arg Val Gly Lys Ala Val Glu

325 330 335

Glu Ala Lys Ala Thr Gly His Ile Lys Val Ile Thr Gly Gly Glu Lys

340 345 350

Arg Lys Gly Asn Gly Tyr Tyr Tyr Ala Pro Thr Leu Leu Ala Gly Ala

355 360 365

Leu Gln Asp Asp Ala Ile Val Gln Lys Glu Val Phe Gly Pro Val Val

370 375 380

Ser Val Thr Pro Phe Asp Asn Glu Glu Gln Val Val Asn Trp Ala Asn

385 390 395 400

Asp Ser Gln Tyr Gly Leu Ala Ser Ser Val Trp Thr Lys Asp Val Gly

405 410 415

Arg Ala His Arg Val Ser Ala Arg Leu Gln Tyr Gly Cys Thr Trp Val

420 425 430

Asn Thr His Phe Met Leu Val Ser Glu Met Pro His Gly Gly Gln Lys

435 440 445

Leu Ser Gly Tyr Gly Lys Asp Met Ser Leu Tyr Gly Leu Glu Asp Tyr

450 455 460

Thr Val Val Arg His Val Met Val Lys His

465 470

<210> 53

<211> 480

<212> PRT

<213>Pseudomonas putida (Pseudomonas putida)

<400> 53

Met Gln Leu Lys Asp Ala Gln Leu Phe Arg Gln Gln Ala Tyr Ile Asn

1 5 10 15

Gly Glu Trp Leu Asp Ala Asp Asn Gly Gln Thr Ile Lys Val Thr Asn

20 25 30

Pro Ala Thr Gly Glu Val Ile Gly Thr Val Pro Lys Met Gly Thr Ala

35 40 45

Glu Thr Arg Arg Ala Ile Glu Ala Ala Asp Lys Ala Leu Pro Ala Trp

50 55 60

Arg Ala Leu Thr Ala Lys Glu Arg Ser Ala Lys Leu Arg Arg Trp Phe

65 70 75 80

Glu Leu Met Ile Glu Asn Gln Asp Asp Leu Ala Arg Leu Met Thr Thr

85 90 95

Glu Gln Gly Lys Pro Leu Ala Glu Ala Lys Gly Glu Ile Ala Tyr Ala

100 105 110

Ala Ser Phe Ile Glu Trp Phe Ala Glu Glu Ala Lys Arg Ile Tyr Gly

115 120 125

Asp Thr Ile Pro Gly His Gln Pro Asp Lys Arg Leu Ile Val Ile Lys

130 135 140

Gln Pro Ile Gly Val Thr Ala Ala Ile Thr Pro Trp Asn Phe Pro Ala

145 150 155 160

Ala Met Ile Thr Arg Lys Ala Gly Pro Ala Leu Ala Ala Gly Cys Thr

165 170 175

Met Val Leu Lys Pro Ala Ser Gln Thr Pro Tyr Ser Ala Leu Ala Leu

180 185 190

Val Glu Leu Ala His Arg Ala Gly Ile Pro Ala Gly Val Leu Ser Val

195 200 205

Val Thr Gly Ser Ala Gly Glu Val Gly Gly Glu Leu Thr Gly Asn Ser

210 215 220

Leu Val Arg Lys Leu Ser Phe Thr Gly Ser Thr Glu Ile Gly Arg Gln

225 230 235 240

Leu Met Glu Glu Cys Ala Lys Asp Ile Lys Lys Val Ser Leu Glu Leu

245 250 255

Gly Gly Asn Ala Pro Phe Ile Val Phe Asp Asp Ala Asp Leu Asp Lys

260 265 270

Ala Val Glu Gly Ala Ile Ile Ser Lys Tyr Arg Asn Asn Gly Gln Thr

275 280 285

Cys Val Cys Ala Asn Arg Ile Tyr Val Gln Asp Gly Val Tyr Asp Ala

290 295 300

Phe Ala Glu Lys Leu Ala Ala Ala Val Ala Lys Leu Lys Ile Gly Asn

305 310 315 320

Gly Leu Glu Glu Gly Thr Thr Thr Gly Pro Leu Ile Asp Gly Lys Ala

325 330 335

Val Ala Lys Val Gln Glu His Ile Glu Asp Ala Val Ser Lys Gly Ala

340 345 350

Lys Val Leu Ser Gly Gly Lys Leu Ile Glu Gly Asn Phe Phe Glu Pro

355 360 365

Thr Ile Leu Val Asp Val Pro Lys Thr Ala Ala Val Ala Lys Glu Glu

370 375 380

Thr Phe Gly Pro Leu Ala Pro Leu Phe Arg Phe Lys Asp Glu Ala Glu

385 390 395 400

Val Ile Ala Met Ser Asn Asp Thr Glu Phe Gly Leu Ala Ser Tyr Phe

405 410 415

Tyr Ala Arg Asp Met Ser Arg Val Phe Arg Val Ala Glu Ala Leu Glu

420 425 430

Tyr Gly Met Val Gly Ile Asn Thr Gly Leu Ile Ser Asn Glu Val Ala

435 440 445

Pro Phe Gly Gly Ile Lys Ala Ser Gly Leu Gly Arg Glu Gly Ser Lys

450 455 460

Tyr Gly Ile Glu Asp Tyr Leu Glu Ile Lys Tyr Leu Cys Ile Ser Val

465 470 475 480

<210> 54

<211> 479

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 54

Met Ser Val Pro Val Gln His Pro Met Tyr Ile Asp Gly Gln Phe Val

1 5 10 15

Thr Trp Arg Gly Asp Ala Trp Ile Asp Val Val Asn Pro Ala Thr Glu

20 25 30

Ala Val Ile Ser Arg Ile Pro Asp Gly Gln Ala Glu Asp Ala Arg Lys

35 40 45

Ala Ile Asp Ala Ala Glu Arg Ala Gln Pro Glu Trp Glu Ala Leu Pro

50 55 60

Ala Ile Glu Arg Ala Ser Trp Leu Arg Lys Ile Ser Ala Gly Ile Arg

65 70 75 80

Glu Arg Ala Ser Glu Ile Ser Ala Leu Ile Val Glu Glu Gly Gly Lys

85 90 95

Ile Gln Gln Leu Ala Glu Val Glu Val Ala Phe Thr Ala Asp Tyr Ile

100 105 110

Asp Tyr Met Ala Glu Trp Ala Arg Arg Tyr Glu Gly Glu Ile Ile Gln

115 120 125

Ser Asp Arg Pro Gly Glu Asn Ile Leu Leu Phe Lys Arg Ala Leu Gly

130 135 140

Val Thr Thr Gly Ile Leu Pro Trp Asn Phe Pro Phe Phe Leu Ile Ala

145 150 155 160

Arg Lys Met Ala Pro Ala Leu Leu Thr Gly Asn Thr Ile Val Ile Lys

165 170 175

Pro Ser Glu Phe Thr Pro Asn Asn Ala Ile Ala Phe Ala Lys Ile Val

180 185 190

Asp Glu Ile Gly Leu Pro Arg Gly Val Phe Asn Leu Val Leu Gly Arg

195 200 205

Gly Glu Thr Val Gly Gln Glu Leu Ala Gly Asn Pro Lys Val Ala Met

210 215 220

Val Ser Met Thr Gly Ser Val Ser Ala Gly Glu Lys Ile Met Ala Thr

225 230 235 240

Ala Ala Lys Asn Ile Thr Lys Val Cys Leu Glu Leu Gly Gly Lys Ala

245 250 255

Pro Ala Ile Val Met Asp Asp Ala Asp Leu Glu Leu Ala Val Lys Ala

260 265 270

Ile Val Asp Ser Arg Val Ile Asn Ser Gly Gln Val Cys Asn Cys Ala

275 280 285

Glu Arg Val Tyr Val Gln Lys Gly Ile Tyr Asp Gln Phe Val Asn Arg

290 295 300

Leu Gly Glu Ala Met Gln Ala Val Gln Phe Gly Asn Pro Ala Glu Arg

305 310 315 320

Asn Asp Ile Ala Met Gly Pro Leu Ile Asn Ala Ala Ala Leu Glu Arg

325 330 335

Val Glu Gln Lys Val Ala Arg Ala Val Glu Glu Gly Ala Arg Val Ala

340 345 350

Phe Gly Gly Lys Ala Val Glu Gly Lys Gly Tyr Tyr Tyr Pro Pro Thr

355 360 365

Leu Leu Leu Asp Val Arg Gln Glu Met Ser Ile Met His Glu Glu Thr

370 375 380

Phe Gly Pro Val Leu Pro Val Val Ala Phe Asp Thr Leu Glu Asp Ala

385 390 395 400

Ile Ser Met Ala Asn Asp Ser Asp Tyr Gly Leu Thr Ser Ser Ile Tyr

405 410 415

Thr Gln Asn Leu Asn Val Ala Met Lys Ala Ile Lys Gly Leu Lys Phe

420 425 430

Gly Glu Thr Tyr Ile Asn Arg Glu Asn Phe Glu Ala Met Gln Gly Phe

435 440 445

His Ala Gly Trp Arg Lys Ser Gly Ile Gly Gly Ala Asp Gly Lys His

450 455 460

Gly Leu His Glu Tyr Leu Gln Thr Gln Val Val Tyr Leu Gln Ser

465 470 475

<210> 55

<211> 512

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 55

Met Thr Asn Asn Pro Pro Ser Ala Gln Ile Lys Pro Gly Glu Tyr Gly

1 5 10 15

Phe Pro Leu Lys Leu Lys Ala Arg Tyr Asp Asn Phe Ile Gly Gly Glu

20 25 30

Trp Val Ala Pro Ala Asp Gly Glu Tyr Tyr Gln Asn Leu Thr Pro Val

35 40 45

Thr Gly Gln Leu Leu Cys Glu Val Ala Ser Ser Gly Lys Arg Asp Ile

50 55 60

Asp Leu Ala Leu Asp Ala Ala His Lys Val Lys Asp Lys Trp Ala His

65 70 75 80

Thr Ser Val Gln Asp Arg Ala Ala Ile Leu Phe Lys Ile Ala Asp Arg

85 90 95

Met Glu Gln Asn Leu Glu Leu Leu Ala Thr Ala Glu Thr Trp Asp Asn

100 105 110

Gly Lys Pro Ile Arg Glu Thr Ser Ala Ala Asp Val Pro Leu Ala Ile

115 120 125

Asp His Phe Arg Tyr Phe Ala Ser Cys Ile Arg Ala Gln Glu Gly Gly

130 135 140

Ile Ser Glu Val Asp Ser Glu Thr Val Ala Tyr His Phe His Glu Pro

145 150 155 160

Leu Gly Val Val Gly Gln Ile Ile Pro Trp Asn Phe Pro Leu Leu Met

165 170 175

Ala Ser Trp Lys Met Ala Pro Ala Leu Ala Ala Gly Asn Cys Val Val

180 185 190

Leu Lys Pro Ala Arg Leu Thr Pro Leu Ser Val Leu Leu Leu Met Glu

195 200 205

Ile Val Gly Asp Leu Leu Pro Pro Gly Val Val Asn Val Val Asn Gly

210 215 220

Ala Gly Gly Val Ile Gly Glu Tyr Leu Ala Thr Ser Lys Arg Ile Ala

225 230 235 240

Lys Val Ala Phe Thr Gly Ser Thr Glu Val Gly Gln Gln Ile Met Gln

245 250 255

Tyr Ala Thr Gln Asn Ile Ile Pro Val Thr Leu Glu Leu Gly Gly Lys

260 265 270

Ser Pro Asn Ile Phe Phe Ala Asp Val Met Asp Glu Glu Asp Ala Phe

275 280 285

Phe Asp Lys Ala Leu Glu Gly Phe Ala Leu Phe Ala Phe Asn Gln Gly

290 295 300

Glu Val Cys Thr Cys Pro Ser Arg Ala Leu Val Gln Glu Ser Ile Tyr

305 310 315 320

Glu Arg Phe Met Glu Arg Ala Ile Arg Arg Val Glu Ser Ile Arg Ser

325 330 335

Gly Asn Pro Leu Asp Ser Val Thr Gln Met Gly Ala Gln Val Ser His

340 345 350

Gly Gln Leu Glu Thr Ile Leu Asn Tyr Ile Asp Ile Gly Lys Lys Glu

355 360 365

Gly Ala Asp Val Leu Thr Gly Gly Arg Arg Lys Leu Leu Glu Gly Glu

370 375 380

Leu Lys Asp Gly Tyr Tyr Leu Glu Pro Thr Ile Leu Phe Gly Gln Asn

385 390 395 400

Asn Met Arg Val Phe Gln Glu Glu Ile Phe Gly Pro Val Leu Ala Val

405 410 415

Thr Thr Phe Lys Thr Met Glu Glu Ala Leu Glu Leu Ala Asn Asp Thr

420 425 430

Gln Tyr Gly Leu Gly Ala Gly Val Trp Ser Arg Asn Gly Asn Leu Ala

435 440 445

Tyr Lys Met Gly Arg Gly Ile Gln Ala Gly Arg Val Trp Thr Asn Cys

450 455 460

Tyr His Ala Tyr Pro Ala His Ala Ala Phe Gly Gly Tyr Lys Gln Ser

465 470 475 480

Gly Ile Gly Arg Glu Thr His Lys Met Met Leu Glu His Tyr Gln Gln

485 490 495

Thr Lys Cys Leu Leu Val Ser Tyr Ser Asp Lys Pro Leu Gly Leu Phe

500 505 510

<210> 56

<211> 463

<212> PRT

<213>Methanococcus jannaschii (Methanocaldococcus jannaschii)

<400> 56

Met Phe Ile Asp Gly Lys Trp Ile Asn Arg Glu Asp Met Asp Val Ile

1 5 10 15

Asn Pro Tyr Ser Leu Glu Val Ile Lys Lys Ile Pro Ala Leu Ser Arg

20 25 30

Glu Glu Ala Lys Glu Ala Ile Asp Thr Ala Glu Lys Tyr Lys Glu Val

35 40 45

Met Lys Asn Leu Pro Ile Thr Lys Arg Tyr Asn Ile Leu Met Asn Ile

50 55 60

Ala Lys Gln Ile Lys Glu Lys Lys Glu Glu Leu Ala Lys Ile Leu Ala

65 70 75 80

Ile Asp Ala Gly Lys Pro Ile Lys Gln Ala Arg Val Glu Val Glu Arg

85 90 95

Ser Ile Gly Thr Phe Lys Leu Ala Ala Phe Tyr Val Lys Glu His Arg

100 105 110

Asp Glu Val Ile Pro Ser Asp Asp Arg Leu Ile Phe Thr Arg Arg Glu

115 120 125

Pro Val Gly Ile Val Gly Ala Ile Thr Pro Phe Asn Phe Pro Leu Asn

130 135 140

Leu Ser Ala His Lys Ile Ala Pro Ala Ile Ala Thr Gly Asn Val Ile

145 150 155 160

Val His His Pro Ser Ser Lys Ala Pro Leu Val Cys Ile Glu Leu Ala

165 170 175

Lys Ile Ile Glu Asn Ala Leu Lys Lys Tyr Asn Val Pro Leu Gly Val

180 185 190

Tyr Asn Leu Leu Thr Gly Ala Gly Glu Val Val Gly Asp Glu Ile Val

195 200 205

Val Asn Glu Lys Val Asn Met Ile Ser Phe Thr Gly Ser Ser Lys Val

210 215 220

Gly Glu Leu Ile Thr Lys Lys Ala Gly Phe Lys Lys Ile Ala Leu Glu

225 230 235 240

Leu Gly Gly Val Asn Pro Asn Ile Val Leu Lys Asp Ala Asp Leu Asn

245 250 255

Lys Ala Val Asn Ala Leu Ile Lys Gly Ser Phe Ile Tyr Ala Gly Gln

260 265 270

Val Cys Ile Ser Val Gly Met Ile Leu Val Asp Glu Ser Ile Ala Asp

275 280 285

Lys Phe Ile Glu Met Phe Val Asn Lys Ala Lys Val Leu Asn Val Gly

290 295 300

Asn Pro Leu Asp Glu Lys Thr Asp Val Gly Pro Leu Ile Ser Val Glu

305 310 315 320

His Ala Glu Trp Val Glu Lys Val Val Glu Lys Ala Ile Asp Glu Gly

325 330 335

Gly Lys Leu Leu Leu Gly Gly Lys Arg Asp Lys Ala Leu Phe Tyr Pro

340 345 350

Thr Ile Leu Glu Val Asp Arg Asp Asn Ile Leu Cys Lys Thr Glu Thr

355 360 365

Phe Ala Pro Val Ile Pro Ile Ile Arg Thr Asn Glu Glu Glu Met Ile

370 375 380

Asp Ile Ala Asn Ser Thr Glu Tyr Gly Leu His Ser Ala Ile Phe Thr

385 390 395 400

Asn Asp Ile Asn Lys Ser Leu Lys Phe Ala Glu Asn Leu Glu Phe Gly

405 410 415

Gly Val Val Ile Asn Asp Ser Ser Leu Phe Arg Gln Asp Asn Met Pro

420 425 430

Phe Gly Gly Val Lys Lys Ser Gly Leu Gly Arg Glu Gly Val Lys Tyr

435 440 445

Ala Met Glu Glu Met Ser Asn Ile Lys Thr Ile Ile Ile Ser Lys

450 455 460

<210> 57

<211> 488

<212> PRT

<213>Bacillus licheniformis (Bacillus licheniformis)

<400> 57

Met Ser Val Ala Ala Glu Ser Lys Thr Tyr Phe Asn Phe Ile Asn Gly

1 5 10 15

Arg Trp Val Lys Ala Glu Ser Gly Gly Met Glu Gln Ser Leu Asn Pro

20 25 30

Ala Asp Thr Arg Asp Ile Val Gly Leu Val Gln Lys Ser Ser Ile Glu

35 40 45

Asp Val Asp Arg Ala Val Glu Ala Ala Lys Gln Ala Lys Lys Ala Trp

50 55 60

Arg Lys Leu Ser Gly Ala Glu Arg Gly Gln Phe Leu Tyr Lys Ala Ala

65 70 75 80

Asp Ile Met Glu Gln Arg Leu Asp Glu Ile Ala Glu Cys Ala Thr Arg

85 90 95

Glu Met Gly Lys Thr Leu Pro Glu Ala Lys Gly Glu Thr Ala Arg Gly

100 105 110

Ile Ala Ile Leu Arg Tyr Tyr Ala Gly Glu Gly Leu Arg Lys Thr Gly

115 120 125

Asp Val Ile Pro Ser Thr Asp Ser Ser Ala Phe Met Tyr Thr Asp Arg

130 135 140

Val Pro Leu Gly Val Val Gly Val Ile Ser Pro Trp Asn Phe Pro Val

145 150 155 160

Ala Ile Pro Ile Trp Lys Met Ala Pro Ala Leu Ile Tyr Gly Asn Thr

165 170 175

Val Val Ile Lys Pro Ala Thr Glu Thr Ala Val Thr Cys Leu Lys Val

180 185 190

Ile Ser Cys Phe Glu Glu Ala Gly Ile Pro Ser Gly Val Val Asn Ala

195 200 205

Val Thr Gly Pro Gly Ser Ser Ala Gly Gln Arg Leu Ala Glu His Pro

210 215 220

Asp Val Asn Gly Ile Thr Phe Thr Gly Ser Asn Gln Thr Gly Lys Ile

225 230 235 240

Ile Gly Arg Thr Ala Phe Glu Arg Gly Ala Lys Tyr Gln Leu Glu Met

245 250 255

Gly Gly Lys Asn Pro Val Ile Val Ala Asp Asp Ala Asp Leu Asp Ile

260 265 270

Ala Val Glu Ala Val Ile Ser Gly Ala Phe Arg Ser Thr Gly Gln Lys

275 280 285

Cys Thr Ala Thr Ser Arg Val Ile Val Leu Asn Gly Val Tyr Asp Arg

290 295 300

Phe Lys Glu Lys Leu Leu Gln Gln Thr Lys Glu Ile Thr Ile Gly Asp

305 310 315 320

Ser Leu Lys Glu Asp Val Trp Met Gly Pro Ile Ala Asn Lys Gln Gln

325 330 335

Leu Asp Asn Cys Leu Ser Tyr Ile Ala Lys Gly Lys Gln Glu Gly Ala

340 345 350

Asp Leu Ile Phe Gly Gly Glu Arg Leu Ala Asp Gly Lys Tyr Glu Asn

355 360 365

Gly Tyr Tyr Ile Arg Pro Ala Ile Phe Asp Asn Val Thr Ser Gly Met

370 375 380

Thr Ile Ala Gln Glu Glu Ile Phe Gly Pro Val Ile Ala Leu Ile Lys

385 390 395 400

Ala Asp Thr Leu Glu Glu Ala Leu Glu Thr Ala Asn Asp Val Lys Phe

405 410 415

Gly Leu Ser Ala Ser Ile Phe Thr Gln Asn Ile Arg Arg Met Leu Ser

420 425 430

Phe Thr Asp Glu Ile Glu Ala Gly Leu Ile Arg Val Asn Ala Glu Ser

435 440 445

Ala Gly Val Glu Leu Gln Ala Pro Phe Gly Gly Val Lys Gln Ser Ser

450 455 460

Ser His Ser Arg Glu Gln Gly Glu Ala Ala Lys Glu Phe Phe Thr Ala

465 470 475 480

Val Lys Thr Val Phe Val Lys Pro

485

<210> 58

<211> 491

<212> PRT

<213>Pseudomonas strain (Pseudomonas sp.)

<400> 58

Met Lys Gln Tyr Arg Asn Phe Val Asp Gly Lys Trp Val Glu Ser Ser

1 5 10 15

Lys Thr Phe Gln Asp Val Thr Pro Ile Asp Gly Ser Val Val Ala Val

20 25 30

Val His Glu Ala Asp Arg Asp Leu Val Asp Ala Ala Val Lys Ala Gly

35 40 45

His Arg Ala Leu Glu Gly Glu Trp Gly Arg Thr Thr Ala Ala Gln Arg

50 55 60

Val Asp Trp Leu Arg Arg Ile Ala Asn Glu Met Glu Arg Arg Gln Gln

65 70 75 80

Asp Phe Leu Asp Ala Glu Met Ala Asp Thr Gly Lys Pro Leu Ser Met

85 90 95

Ala Ala Thr Ile Asp Ile Pro Arg Gly Ile Ala Asn Phe Arg Asn Phe

100 105 110

Ala Asp Ile Leu Ala Thr Ala Pro Val Asp Ser His Arg Leu Asp Leu

115 120 125

Pro Asp Gly Ala Tyr Ala Leu Asn Tyr Ala Ala Arg Lys Pro Leu Gly

130 135 140

Val Val Gly Val Ile Ser Pro Trp Asn Leu Pro Leu Leu Leu Leu Thr

145 150 155 160

Trp Lys Val Ala Pro Ala Leu Ala Cys Gly Asn Ala Val Val Val Lys

165 170 175

Pro Ser Glu Asp Thr Pro Gly Thr Ala Thr Leu Leu Ala Glu Val Met

180 185 190

Glu Ala Val Gly Ile Pro Pro Gly Val Phe Asn Leu Val His Gly Phe

195 200 205

Gly Pro Asn Ser Ala Gly Glu Phe Ile Ser Gln His Pro Asp Ile Ser

210 215 220

Ala Ile Thr Phe Thr Gly Glu Ser Lys Thr Gly Ser Thr Ile Met Arg

225 230 235 240

Ala Ala Ala Glu Gly Val Lys Pro Val Ser Phe Glu Leu Gly Gly Lys

245 250 255

Asn Ala Ala Val Ile Phe Ala Asp Cys Asp Phe Glu Lys Met Leu Asp

260 265 270

Gly Met Met Arg Ala Leu Phe Leu Asn Ser Gly Gln Val Cys Leu Cys

275 280 285

Ser Glu Arg Val Tyr Val Glu Arg Pro Ile Phe Asp Arg Phe Cys Val

290 295 300

Ala Leu Ala Glu Arg Ile Lys Ala Leu Lys Val Asp Trp Pro His Glu

305 310 315 320

Thr Asp Thr Gln Met Gly Pro Leu Ile Ser Ser Lys His Arg Asp Lys

325 330 335

Val Leu Ser Tyr Phe Glu Leu Ala Arg Gln Glu Gly Ala Thr Phe Leu

340 345 350

Ala Gly Gly Gly Val Pro Arg Phe Gly Asp Glu Arg Asp Asn Gly Ala

355 360 365

Trp Val Glu Pro Thr Val Ile Ala Gly Leu Ser Asp Asp Ala Arg Val

370 375 380

Val Arg Glu Glu Ile Phe Gly Pro Ile Cys His Val Thr Pro Phe Asp

385 390 395 400

Ser Glu Ser Glu Val Ile Arg Arg Ala Asn Asp Thr Arg Tyr Gly Leu

405 410 415

Ala Ala Thr Ile Trp Thr Thr Asn Leu Ser Arg Ala His Arg Val Ser

420 425 430

Glu Leu Met Arg Val Gly Ile Ser Trp Val Asn Thr Trp Phe Leu Arg

435 440 445

Asp Leu Arg Thr Pro Phe Gly Gly Ala Gly Leu Ser Gly Ile Gly Arg

450 455 460

Glu Gly Gly Met His Ser Leu Asn Phe Tyr Ser Glu Leu Thr Asn Val

465 470 475 480

Cys Val Arg Ile Asp Lys Glu Ser Pro Asp Val

485 490

<210> 59

<211> 499

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 59

Met Thr Glu Pro His Val Ala Val Leu Ser Gln Val Gln Gln Phe Leu

1 5 10 15

Asp Arg Gln His Gly Leu Tyr Ile Asp Gly Arg Pro Gly Pro Ala Gln

20 25 30

Ser Glu Lys Arg Leu Ala Ile Phe Asp Pro Ala Thr Gly Gln Glu Ile

35 40 45

Ala Ser Thr Ala Asp Ala Asn Glu Ala Asp Val Asp Asn Ala Val Met

50 55 60

Ser Ala Trp Arg Ala Phe Val Ser Arg Arg Trp Ala Gly Arg Leu Pro

65 70 75 80

Ala Glu Arg Glu Arg Ile Leu Leu Arg Phe Ala Asp Leu Val Glu Gln

85 90 95

His Ser Glu Glu Leu Ala Gln Leu Glu Thr Leu Glu Gln Gly Lys Ser

100 105 110

Ile Ala Ile Ser Arg Ala Phe Glu Val Gly Cys Thr Leu Asn Trp Met

115 120 125

Arg Tyr Thr Ala Gly Leu Thr Thr Lys Ile Ala Gly Lys Thr Leu Asp

130 135 140

Leu Ser Ile Pro Leu Pro Gln Gly Ala Arg Tyr Gln Ala Trp Thr Arg

145 150 155 160

Lys Glu Pro Val Gly Val Val Ala Gly Ile Val Pro Trp Asn Phe Pro

165 170 175

Leu Met Ile Gly Met Trp Lys Val Met Pro Ala Leu Ala Ala Gly Cys

180 185 190

Ser Ile Val Ile Lys Pro Ser Glu Thr Thr Pro Leu Thr Met Leu Arg

195 200 205

Val Ala Glu Leu Ala Ser Glu Ala Gly Ile Pro Asp Gly Val Phe Asn

210 215 220

Val Val Thr Gly Ser Gly Ala Val Cys Gly Ala Ala Leu Thr Ser His

225 230 235 240

Pro His Val Ala Lys Ile Ser Phe Thr Gly Ser Thr Ala Thr Gly Lys

245 250 255

Gly Ile Ala Arg Thr Ala Ala Asp His Leu Thr Arg Val Thr Leu Glu

260 265 270

Leu Gly Gly Lys Asn Pro Ala Ile Val Leu Lys Asp Ala Asp Pro Gln

275 280 285

Trp Val Ile Glu Gly Leu Met Thr Gly Ser Phe Leu Asn Gln Gly Gln

290 295 300

Val Cys Ala Ala Ser Ser Arg Ile Tyr Ile Glu Ala Pro Leu Phe Asp

305 310 315 320

Thr Leu Val Ser Gly Phe Glu Gln Ala Val Lys Ser Leu Gln Val Gly

325 330 335

Pro Gly Met Ser Pro Val Ala Gln Ile Asn Pro Leu Val Ser Arg Ala

340 345 350

His Cys Asp Lys Val Cys Ser Phe Leu Asp Asp Ala Gln Ala Gln Gln

355 360 365

Ala Glu Leu Ile Arg Gly Ser Asn Gly Pro Ala Gly Glu Gly Tyr Tyr

370 375 380

Val Ala Pro Thr Leu Val Val Asn Pro Asp Ala Lys Leu Arg Leu Thr

385 390 395 400

Arg Glu Glu Val Phe Gly Pro Val Val Asn Leu Val Arg Val Ala Asp

405 410 415

Gly Glu Glu Ala Leu Gln Leu Ala Asn Asp Thr Glu Tyr Gly Leu Thr

420 425 430

Ala Ser Val Trp Thr Gln Asn Leu Ser Gln Ala Leu Glu Tyr Ser Asp

435 440 445

Arg Leu Gln Ala Gly Thr Val Trp Val Asn Ser His Thr Leu Ile Asp

450 455 460

Ala Asn Leu Pro Phe Gly Gly Met Lys Gln Ser Gly Thr Gly Arg Asp

465 470 475 480

Phe Gly Pro Asp Trp Leu Asp Gly Trp Cys Glu Thr Lys Ser Val Cys

485 490 495

Val Arg Tyr

<210> 60

<211> 492

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 60

Met Thr Leu Trp Ile Asn Gly Asp Trp Ile Thr Gly Gln Gly Ala Ser

1 5 10 15

Arg Val Lys Arg Asn Pro Val Ser Gly Glu Val Leu Trp Gln Gly Asn

20 25 30

Asp Ala Asp Ala Ala Gln Val Glu Gln Ala Cys Arg Ala Ala Arg Ala

35 40 45

Ala Phe Pro Arg Trp Ala Arg Leu Ser Phe Ala Glu Arg His Ala Val

50 55 60

Val Glu Arg Phe Ala Ala Leu Leu Glu Ser Asn Lys Ala Glu Leu Thr

65 70 75 80

Ala Ile Ile Ala Arg Glu Thr Gly Lys Pro Arg Trp Glu Ala Ala Thr

85 90 95

Glu Val Thr Ala Met Ile Asn Lys Ile Ala Ile Ser Ile Lys Ala Tyr

100 105 110

His Val Arg Thr Gly Glu Gln Arg Ser Glu Met Pro Asp Gly Ala Ala

115 120 125

Ser Leu Arg His Arg Pro His Gly Val Leu Ala Val Phe Gly Pro Tyr

130 135 140

Asn Phe Pro Gly His Leu Pro Asn Gly His Ile Val Pro Ala Leu Leu

145 150 155 160

Ala Gly Asn Thr Ile Ile Phe Lys Pro Ser Glu Leu Thr Pro Trp Ser

165 170 175

Gly Glu Ala Val Met Arg Leu Trp Gln Gln Ala Gly Leu Pro Pro Gly

180 185 190

Val Leu Asn Leu Val Gln Gly Gly Arg Glu Thr Gly Gln Ala Leu Ser

195 200 205

Ala Leu Glu Asp Leu Asp Gly Leu Leu Phe Thr Gly Ser Ala Asn Thr

210 215 220

Gly Tyr Gln Leu His Arg Gln Leu Ser Gly Gln Pro Glu Lys Ile Leu

225 230 235 240

Ala Leu Glu Met Gly Gly Asn Asn Pro Leu Ile Ile Asp Glu Val Ala

245 250 255

Asp Ile Asp Ala Ala Val His Leu Thr Ile Gln Ser Ala Phe Val Thr

260 265 270

Ala Gly Gln Arg Cys Thr Cys Ala Arg Arg Leu Leu Leu Lys Ser Gly

275 280 285

Ala Gln Gly Asp Ala Phe Leu Ala Arg Leu Val Ala Val Ser Gln Arg

290 295 300

Leu Thr Pro Gly Asn Trp Asp Asp Glu Pro Gln Pro Phe Ile Gly Gly

305 310 315 320

Leu Ile Ser Glu Gln Ala Ala Gln Gln Val Val Thr Ala Trp Gln Gln

325 330 335

Leu Glu Ala Met Gly Gly Arg Pro Leu Leu Ala Pro Arg Leu Leu Gln

340 345 350

Ala Gly Thr Ser Leu Leu Thr Pro Gly Ile Ile Glu Met Thr Gly Val

355 360 365

Ala Gly Val Pro Asp Glu Glu Val Phe Gly Pro Leu Leu Arg Val Trp

370 375 380

Arg Tyr Asp Thr Phe Asp Glu Ala Ile Arg Met Ala Asn Asn Thr Arg

385 390 395 400

Phe Gly Leu Ser Cys Gly Leu Val Ser Pro Glu Arg Glu Lys Phe Asp

405 410 415

Gln Leu Leu Leu Glu Ala Arg Ala Gly Ile Val Asn Trp Asn Lys Pro

420 425 430

Leu Thr Gly Ala Ala Ser Thr Ala Pro Phe Gly Gly Ile Gly Ala Ser

435 440 445

Gly Asn His Arg Pro Ser Ala Trp Tyr Ala Ala Asp Tyr Cys Ala Trp

450 455 460

Pro Met Ala Ser Leu Glu Ser Asp Ser Leu Thr Leu Pro Ala Thr Leu

465 470 475 480

Asn Pro Gly Leu Asp Phe Ser Asp Glu Val Val Arg

485 490

<210> 61

<211> 1358

<212> PRT

<213>Corn (Zea mays) (maize (Maize))

<400> 61

Met Gly Lys Glu Ala Gly Ala Ala Glu Ser Ser Thr Val Val Leu Ala

1 5 10 15

Val Asn Gly Lys Arg Tyr Glu Ala Ala Gly Val Ala Pro Ser Thr Ser

20 25 30

Leu Leu Glu Phe Leu Arg Thr Gln Thr Pro Val Arg Gly Pro Lys Leu

35 40 45

Gly Cys Gly Glu Gly Gly Cys Gly Ala Cys Val Val Leu Val Ser Lys

50 55 60

Tyr Asp Pro Ala Thr Asp Glu Val Thr Glu Phe Ser Ala Ser Ser Cys

65 70 75 80

Leu Thr Leu Leu His Ser Val Asp Arg Cys Ser Val Thr Thr Ser Glu

85 90 95

Gly Ile Gly Asn Thr Arg Asp Gly Tyr His Pro Val Gln Gln Arg Leu

100 105 110

Ser Gly Phe His Ala Ser Gln Cys Gly Phe Cys Thr Pro Gly Met Cys

115 120 125

Met Ser Ile Phe Ser Ala Leu Val Lys Ala Asp Asn Lys Ser Asp Arg

130 135 140

Pro Asp Pro Pro Ala Gly Phe Ser Lys Ile Thr Thr Ser Glu Ala Glu

145 150 155 160

Lys Ala Val Ser Gly Asn Leu Cys Arg Cys Thr Gly Tyr Arg Pro Ile

165 170 175

Val Asp Thr Cys Lys Ser Phe Ala Ser Asp Val Asp Leu Glu Asp Leu

180 185 190

Gly Leu Asn Cys Phe Trp Lys Lys Gly Glu Glu Pro Ala Glu Val Ser

195 200 205

Arg Leu Pro Gly Tyr Asn Ser Gly Ala Val Cys Thr Phe Pro Glu Phe

210 215 220

Leu Lys Ser Glu Ile Lys Ser Thr Met Lys Gln Val Asn Asp Val Pro

225 230 235 240

Ile Ala Ala Ser Gly Asp Gly Trp Tyr His Pro Lys Ser Ile Glu Glu

245 250 255

Leu His Arg Leu Phe Asp Ser Ser Trp Phe Asp Asp Ser Ser Val Lys

260 265 270

Ile Val Ala Ser Asn Thr Gly Ser Gly Val Tyr Lys Asp Gln Asp Leu

275 280 285

Tyr Asp Lys Tyr Ile Asp Ile Lys Gly Ile Pro Glu Leu Ser Val Ile

290 295 300

Asn Lys Asn Asp Lys Ala Ile Glu Leu Gly Ser Val Val Ser Ile Ser

305 310 315 320

Lys Ala Ile Glu Val Leu Ser Asp Gly Asn Leu Val Phe Arg Lys Ile

325 330 335

Ala Asp His Leu Asn Lys Val Ala Ser Pro Phe Val Arg Asn Thr Ala

340 345 350

Thr Ile Gly Gly Asn Ile Met Met Ala Gln Arg Leu Pro Phe Glu Ser

355 360 365

Asp Val Ala Thr Val Leu Leu Ala Ala Gly Ser Thr Val Thr Val Gln

370 375 380

Val Ala Ser Lys Arg Leu Cys Phe Thr Leu Glu Glu Phe Leu Glu Gln

385 390 395 400

Pro Pro Cys Asp Ser Arg Thr Leu Leu Leu Ser Ile Phe Ile Pro Glu

405 410 415

Trp Gly Ser Asp Tyr Val Thr Phe Glu Thr Phe Arg Ala Ala Pro Arg

420 425 430

Pro Phe Gly Asn Ala Val Ser Tyr Val Asn Ser Ala Phe Leu Ala Arg

435 440 445

Thr Ser Gly Ser Leu Leu Ile Glu Asp Ile Cys Leu Ala Phe Gly Ala

450 455 460

Tyr Gly Val Asp His Ala Ile Arg Ala Lys Lys Val Glu Asp Phe Leu

465 470 475 480

Lys Gly Lys Ser Leu Ser Ser Phe Val Ile Leu Glu Ala Ile Lys Leu

485 490 495

Leu Lys Asp Thr Val Ser Pro Ser Glu Gly Thr Thr His His Glu Tyr

500 505 510

Arg Val Ser Leu Ala Val Ser Phe Leu Phe Ser Phe Leu Ser Ser Leu

515 520 525

Ala Asn Ser Ser Ser Ala Pro Ser Asn Ile Asp Thr Pro Asn Gly Ser

530 535 540

Tyr Thr His Glu Thr Gly Ser Asn Val Asp Ser Pro Glu Arg His Ile

545 550 555 560

Lys Val Asp Ser Asn Asp Leu Pro Ile Arg Ser Arg Gln Glu Met Val

565 570 575

Phe Ser Asp Glu Tyr Lys Pro Val Gly Lys Pro Ile Lys Lys Val Gly

580 585 590

Ala Glu Ile Gln Ala Ser Gly Glu Ala Val Tyr Val Asp Asp Ile Pro

595 600 605

Ala Pro Lys Asp Cys Leu Tyr Gly Ala Phe Ile Tyr Ser Thr His Pro

610 615 620

His Ala His Val Arg Ser Ile Asn Phe Lys Ser Ser Leu Ala Ser Gln

625 630 635 640

Lys Val Ile Thr Val Ile Thr Ala Lys Asp Ile Pro Ser Gly Gly Glu

645 650 655

Asn Ile Gly Ser Ser Phe Leu Met Gln Gly Glu Ala Leu Phe Ala Asp

660 665 670

Pro Ile Ala Glu Phe Ala Gly Gln Asn Ile Gly Val Val Ile Ala Glu

675 680 685

Thr Gln Arg Tyr Ala Asn Met Ala Ala Lys Gln Ala Val Val Glu Tyr

690 695 700

Ser Thr Glu Asn Leu Gln Pro Pro Ile Leu Thr Ile Glu Asp Ala Ile

705 710 715 720

Gln Arg Asn Ser Tyr Ile Gln Ile Pro Pro Phe Leu Ala Pro Lys Pro

725 730 735

Val Gly Asp Tyr Asn Lys Gly Met Ala Glu Ala Asp His Lys Ile Leu

740 745 750

Ser Ala Glu Val Lys Leu Glu Ser Gln Tyr Tyr Phe Tyr Met Glu Thr

755 760 765

Gln Ala Ala Leu Ala Ile Pro Asp Glu Asp Asn Cys Ile Thr Ile Tyr

770 775 780

Ser Ser Thr Gln Met Pro Glu Leu Thr Gln Asn Leu Ile Ala Arg Cys

785 790 795 800

Leu Gly Ile Pro Phe His Asn Val Arg Val Ile Ser Arg Arg Val Gly

805 810 815

Gly Gly Phe Gly Gly Lys Ala Met Lys Ala Thr His Thr Ala Cys Ala

820 825 830

Cys Ala Leu Ala Ala Phe Lys Leu Arg Arg Pro Val Arg Met Tyr Leu

835 840 845

Asp Arg Lys Thr Asp Met Ile Met Ala Gly Gly Arg His Pro Met Lys

850 855 860

Ala Lys Tyr Ser Val Gly Phe Lys Ser Asp Gly Lys Ile Thr Ala Leu

865 870 875 880

His Leu Asp Leu Gly Ile Asn Ala Gly Ile Ser Pro Asp Val Ser Pro

885 890 895

Leu Met Pro Arg Ala Ile Ile Gly Ala Leu Lys Lys Tyr Asn Trp Gly

900 905 910

Thr Leu Glu Phe Asp Thr Lys Val Cys Lys Thr Asn Val Ser Ser Lys

915 920 925

Ser Ala Met Arg Ala Pro Gly Asp Val Gln Gly Ser Phe Ile Ala Glu

930 935 940

Ala Ile Ile Glu His Val Ala Ser Ala Leu Ala Leu Asp Thr Asn Thr

945 950 955 960

Val Arg Arg Lys Asn Leu His Asp Phe Glu Ser Leu Glu Val Phe Tyr

965 970 975

Gly Glu Ser Ala Gly Glu Ala Ser Thr Tyr Ser Leu Val Ser Met Phe

980 985 990

Asp Lys Leu Ala Leu Ser Pro Glu Tyr Gln His Arg Ala Ala Met Ile

995 1000 1005

Glu Gln Phe Asn Ser Ser Asn Lys Trp Lys Lys Arg Gly Ile Ser

1010 1015 1020

Cys Val Pro Ala Thr Tyr Glu Val Asn Leu Arg Pro Thr Pro Gly

1025 1030 1035

Lys Val Ser Ile Met Asn Asp Gly Ser Ile Ala Val Glu Val Gly

1040 1045 1050

Gly Ile Glu Ile Gly Gln Gly Leu Trp Thr Lys Val Lys Gln Met

1055 1060 1065

Thr Ala Phe Gly Leu Gly Gln Leu Cys Pro Asp Gly Gly Glu Cys

1070 1075 1080

Leu Leu Asp Lys Val Arg Val Ile Gln Ala Asp Thr Leu Ser Leu

1085 1090 1095

Ile Gln Gly Gly Met Thr Ala Gly Ser Thr Thr Ser Glu Thr Ser

1100 1105 1110

Cys Glu Thr Val Arg Gln Ser Cys Val Ala Leu Val Glu Lys Leu

1115 1120 1125

Asn Pro Ile Lys Glu Ser Leu Glu Ala Lys Ser Asn Thr Val Glu

1130 1135 1140

Trp Ser Ala Leu Ile Ala Gln Ala Ser Met Ala Ser Val Asn Leu

1145 1150 1155

Ser Ala Gln Pro Tyr Trp Thr Pro Asp Pro Ser Phe Lys Ser Tyr

1160 1165 1170

Leu Asn Tyr Gly Ala Gly Thr Ser Glu Val Glu Val Asp Ile Leu

1175 1180 1185

Thr Gly Ala Thr Thr Ile Leu Arg Ser Asp Leu Val Tyr Asp Cys

1190 1195 1200

Gly Gln Ser Leu Asn Pro Ala Val Asp Leu Gly Gln Ile Glu Gly

1205 1210 1215

Cys Phe Val Gln Gly Ile Gly Phe Phe Thr Asn Glu Asp Tyr Lys

1220 1225 1230

Thr Asn Ser Asp Gly Leu Val Ile His Asp Gly Thr Trp Thr Tyr

1235 1240 1245

Lys Ile Pro Thr Val Asp Asn Ile Pro Lys Glu Phe Asn Val Glu

1250 1255 1260

Met Phe Asn Ser Ala Pro Asp Lys Lys Arg Val Leu Ser Ser Lys

1265 1270 1275

Ala Ser Gly Glu Pro Pro Leu Val Leu Ala Thr Ser Val His Cys

1280 1285 1290

Ala Met Arg Glu Ala Ile Arg Ala Ala Arg Lys Glu Phe Ser Val

1295 1300 1305

Ser Thr Ser Pro Ala Lys Ser Ala Val Thr Phe Gln Met Asp Val

1310 1315 1320

Pro Ala Thr Met Pro Val Val Lys Glu Leu Cys Gly Leu Asp Val

1325 1330 1335

Val Glu Arg Tyr Leu Glu Asn Val Ser Ala Ala Ser Ala Gly Pro

1340 1345 1350

Asn Thr Ala Lys Ala

1355

<210> 62

<211> 747

<212> DNA

<213>Crescent shank bacterium (Caulobacter crescentus)

<400> 62

atgtcctcag ccatctatcc cagcctgaag ggcaagcgcg tcgtcatcac cggcggcggc 60

tcgggcatcg gggccggcct caccgccggc ttcgcccgtc agggcgcgga ggtgatcttc 120

ctcgacatcg ccgacgagga ctccagggct cttgaggccg agctggccgg ctcgccgatc 180

ccgccggtct acaagcgctg cgacctgatg aacctcgagg cgatcaaggc ggtcttcgcc 240

gagatcggcg acgtcgacgt gctggtcaac aacgccggca atgacgaccg ccacaagctg 300

gccgacgtga ccggcgccta ttgggacgag cggatcaacg tcaacctgcg ccacatgctg 360

ttctgcaccc aggccgtcgc gccgggcatg aagaagcgtg gcggcggggc ggtgatcaac 420

ttcggttcga tcagctggca cctggggctt gaggacctcg tcctctacga aaccgccaag 480

gccggcatcg aaggcatgac ccgcgcgctg gcccgggagc tgggtcccga cgacatccgc 540

gtcacctgcg tggtgccggg caacgtcaag accaagcgcc aggagaagtg gtacacgccc 600

gaaggcgagg cccagatcgt ggcggcccaa tgcctgaagg gccgcatcgt cccggagaac 660

gtcgccgcgc tggtgctgtt cctggcctcg gatgacgcgt cgctctgcac cggccacgaa 720

tactggatcg acgccggctg gcgttga 747

<210> 63

<211> 870

<212> DNA

<213>Crescent shank bacterium (Caulobacter crescentus)

<400> 63

atgaccgctc aagtcacttg cgtatgggat ctgaaggcca cgttgggcga aggcccgatc 60

tggcatggcg acaccctgtg gttcgtcgac atcaagcagc gtaaaatcca caactaccac 120

cccgccaccg gcgagcgctt cagcttcgac gcgccggatc aggtgacctt cctcgcgccg 180

atcgtcggcg cgaccggctt tgtcgtcggt ctgaagaccg ggattcaccg cttccacccg 240

gccacgggct tcagcctgct gctcgaggtc gaggacgcgg cgctgaacaa ccgccccaac 300

gacgccacgg tcgacgcgca aggccgtctg tggttcggca ccatgcacga cggggaagag 360

aacaatagcg gctcgctcta tcggatggac ctcaccggcg tcgcccggat ggaccgcgac 420

atctgcatca ccaacggccc gtgcgtctcg cccgacggca agaccttcta ccacaccgac 480

accctggaaa agacgatcta cgccttcgac ctggccgagg acggcctgct gtcgaacaag 540

cgcgtcttcg tgcagttcgc cctgggcgac gatgtctatc cggacggttc ggtcgtcgat 600

tccgaaggct atctgtggac cgccctgtgg ggcggtttcg gcgcggtccg cttctcgccg 660

caaggcgacg ccgtgacgcg catcgaactg cccgccccca acgtcaccaa gccctgcttc 720

ggcgggcctg acctgaagac cctctatttc accaccgccc gcaagggcct gagcgacgag 780

accctggccc agtacccgct ggccggcggt gtgttcgccg ttccggtcga tgtggccggc 840

caaccccagc atgaggtccg ccttgtctaa 870

<210> 64

<211> 1968

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 64

atgtctgttc gcaatatttt tgctgacgag agccacgata tttacaccgt cagaacgcac 60

gccgatggcc cggacggcga actcccatta accgcagaga tgcttatcaa ccgcccgagc 120

ggggatctgt tcggtatgac catgaatgcc ggaatgggtt ggtctccgga cgagctggat 180

cgggacggta ttttactgct cagtacactc ggtggcttac gcggcgcaga cggtaaaccc 240

gtggcgctgg cgttgcacca ggggcattac gaactggaca tccagatgaa agcggcggcc 300

gaggttatta aagccaacca tgccctgccc tatgccgtgt acgtctccga tccttgtgac 360

gggcgtactc agggtacaac ggggatgttt gattcgctac cataccgaaa tgacgcatcg 420

atggtaatgc gccgccttat tcgctctctg cccgacgcga aagcagttat tggtgtggcg 480

agttgcgata aggggcttcc ggccaccatg atggcactcg ccgcgcagca caacatcgca 540

accgtgctgg tccccggcgg cgcgacgctg cccgcaaagg atggagaaga caacggcaag 600

gtgcaaacca ttggcgcacg cttcgccaat ggcgaattat ctctacagga cgcacgccgt 660

gcgggctgta aagcctgtgc ctcttccggc ggcggctgtc aatttttggg cactgccggg 720

acatctcagg tggtggccga aggattggga ctggcaatcc cacattcagc cctggcccct 780

tccggtgagc ctgtgtggcg ggagatcgcc agagcttccg cgcgagctgc gctgaacctg 840

agtcaaaaag gcatcaccac ccgggaaatt ctcaccgata aagcgataga gaatgcgatg 900

acggtccatg ccgcgttcgg tggttcaaca aacctgctgt tacacatccc ggcaattgct 960

caccaggcag gttgccatat cccgaccgtt gatgactgga tccgcatcaa caagcgcgtg 1020

ccccgactgg tgagcgtact gcctaatggc ccggtttatc atccaacggt caatgccttt 1080

atggcaggtg gtgtgccgga agtcatgttg catctgcgca gcctcggatt gttgcatgaa 1140

gacgttatga cggttaccgg cagcacgctg aaagaaaacc tcgactggtg ggagcactcc 1200

gaacggcgtc agcggttcaa gcaactcctg ctcgatcagg aacaaatcaa cgctgacgaa 1260

gtgatcatgt ctccgcagca agcaaaagcg cgcggattaa cctcaactat caccttcccg 1320

gtgggcaata ttgcgccaga aggttcggtg atcaaatcca ccgccattga cccctcgatg 1380

attgatgagc aaggtatcta ttaccataaa ggtgtggcga aggtttatct gtccgagaaa 1440

agtgcgattt acgatatcaa acatgacaag atcaaggcgg gcgatattct ggtcattatt 1500

ggcgttggac cttcaggtac agggatggaa gaaacctacc aggttaccag tgccctgaag 1560

catctgtcat acggtaagca tgtttcgtta atcaccgatg cacgtttctc gggcgtttct 1620

actggcgcgt gcatcggcca tgtggggcca gaagcgctgg ccggaggccc catcggtaaa 1680

ttacgcaccg gggatttaat tgaaattaaa attgattgtc gcgagcttca cggcgaagtc 1740

aatttcctcg gaacccgtag cgatgaacaa ttaccttcac aggaggaggc aactgcaata 1800

ttaaatgcca gacccagcca tcaggattta cttcccgatc ctgaattgcc agatgatacc 1860

cggctatggg caatgcttca ggccgtgagt ggtgggacat ggaccggttg tatttatgat 1920

gtaaacaaaa ttggcgcggc tttgcgcgat tttatgaata aaaactga 1968

<210> 65

<211> 1383

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 65

atgacgcaat taaccatgaa agacaaaatt ggctacgggc tgggagacac cgcctgcggc 60

ttcgtctggc aggccacgat gttcctgctg gcctatttct acaccgacgt cttcggcctg 120

tcggcgggga ttatgggcac gctgtttttg gtctcccgcg tgctcgacgc cgtcaccgac 180

ccgctgatgg ggctgctggt agaccgcacc cgcacgcggc acggccagtt ccgcccgttc 240

ctgctgtggg gggccatccc gttcggcatc gtctgcgtgc tgaccttcta cacgccggac 300

ttctccgcac agggcaagat catctacgcc tgcgtgacct acattctcct gaccctggtc 360

tacaccttcg ttaacgtgcc gtactgcgcc atgccgggcg tcatcaccgc cgacccgaaa 420

gagcgtcacg ccctgcagtc ctggcgcttc ttcctggcgg cggcgggctc gctcgctatc 480

agcggcatcg cgctgccgct ggtgagcatc atcggcaaag gggacgagca ggtgggctac 540

ttcggcgcca tgtgcgtgct ggggctgagc ggcgtggtgc tgctctacgt ctgcttcttc 600

acgaccaaag agcgctacac ctttgaggtg cagccgggct cgtcggtggc gaaagacctt 660

aagctgctgc tgggcaacag ccagtggcgc atcatgtgcg cgttcaagat gatggcgacc 720

tgctccaacg tggtgcgcgg cggggcgacg ctctacttcg tgaaatacgt gatggatcac 780

ccggagttgg cgacccagtt tttactttac ggcagcctcg ccaccatgtt cggctcgctt 840

tgctcctcac gcctgctggg ccgcttcgac cgcgtcaccg ccttcaagtg gatcatcgtc 900

gcctactcgc tgatcagcct gctgattttc gtcaccccgg cggagcacat cgcgctcatt 960

tttgccctca acatcctgtt cctgttcgtc tttaatacca ccacgccgct gcagtggctg 1020

atggcttctg acgtggtgga ctacgaggag agccgcagcg gtcgccgcct cgacgggctg 1080

gtgttctcca cctacctgtt cagcctgaag attggcctgg cgattggcgg ggcggtggtg 1140

ggctggatcc tggcgtacgt caactattcc gccagcagca gcgtgcagcc ggttgaggtg 1200

ctcaccacca tcaaaattct gttctgcgtg gtgccggtgg tgctctacgc gggcatgttc 1260

atcatgctgt cgctctacaa gctcaccgat gcccgcgtgg aggccatcag ccggcagctg 1320

attaagcacc gcgcggcgca gggcgaggcc gttcccgacg ccgcgacagc cgcatcccat 1380

taa 1383

<210> 66

<211> 1041

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 66

atgcagaaca tcatccgaaa aggaggaact atgaaggctg cagttgttac gaaggatcat 60

catgttgacg ttacgtataa aacactgcgc tcactgaaac atggcgaagc cctgctgaaa 120

atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga ttttggtgac 180

aaaaccggcg taattctggg ccatgaaggc atcggtgtgg tggcagaagt gggtccaggt 240

gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt ggttctacga aggatgcggt 300

cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa aaatgccgga 360

tacagcgttg atggcgggat ggcggaagag tgcatcgtgg tcgccgatta cgcggtaaaa 420

gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg agtcaccacc 480

tacaaagccg ttaagctgtc aaaaattcgt ccagggcagt ggattgctat ctacggtctt 540

ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg tctttaacgc caaagtgatc 600

gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatgggcgc agatttagcg 660

attaactcac acaccgaaga cgccgccaaa attgtgcagg agaaaactgg tggcgctcac 720

gctgcggtgg taacagcggt agctaaagct gcgtttaact cggcagttga tgctgtccgt 780

gcaggcggtc gtgttgtggc tgtcggtcta ccgccggagt ctatgagcct ggatatccca 840

cgtcttgtgc tggatggtat tgaagtggtc ggttcgctgg tcggcacgcg ccaggattta 900

actgaagcct tccagtttgc cgccgaaggt aaagtggtgc cgaaagtcgc cctgcgtccg 960

ttagcggaca tcaacaccat ctttactgag atggaagaag gcaaaatccg tggccgcatg 1020

gtgattgatt tccgtcacta a 1041

<210> 67

<211> 1164

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 67

atgaacaact ttaatctgca caccccaacc cgcattctgt ttggtaaagg cgcaatcgct 60

ggtttacgcg aacaaattcc tcacgatgct cgcgtattga ttacctacgg cggcggcagc 120

gtgaaaaaaa ccggcgttct cgatcaagtt ctggatgccc tgaaaggcat ggacgtgctg 180

gaatttggcg gtattgagcc aaacccggct tatgaaacgc tgatgaacgc cgtgaaactg 240

gttcgcgaac agaaagtgac tttcctgctg gcggttggcg gcggttctgt actggacggc 300

accaaattta tcgccgcagc ggctaactat ccggaaaata tcgatccgtg gcacattctg 360

caaacgggcg gtaaagagat taaaagcgcc atcccgatgg gctgtgtgct gacgctgcca 420

gcaaccggtt cagaatccaa cgcaggcgcg gtgatctccc gtaaaaccac aggcgacaag 480

caggcgttcc attctgccca tgttcagccg gtatttgccg tgctcgatcc ggtttatacc 540

tacaccctgc cgccgcgtca ggtggctaac ggcgtagtgg acgcctttgt acacaccgtg 600

gaacagtatg ttaccaaacc ggttgatgcc aaaattcagg accgtttcgc agaaggcatt 660

ttgctgacgc taatcgaaga tggtccgaaa gccctgaaag agccagaaaa ctacgatgtg 720

cgcgccaacg tcatgtgggc ggcgactcag gcgctgaacg gtttgattgg cgctggcgta 780

ccgcaggact gggcaacgca tatgctgggc cacgaactga ctgcgatgca cggtctggat 840

cacgcgcaaa cactggctat cgtcctgcct gcactgtgga atgaaaaacg cgataccaag 900

cgcgctaagc tgctgcaata tgctgaacgc gtctggaaca tcactgaagg ttccgatgat 960

gagcgtattg acgccgcgat tgccgcaacc cgcaatttct ttgagcaatt aggcgtgccg 1020

acccacctct ccgactacgg tctggacggc agctccatcc cggctttgct gaaaaaactg 1080

gaagagcacg gcatgaccca actgggcgaa aatcatgaca ttacgttgga tgtcagccgc 1140

cgtatatacg aagccgcccg ctaa 1164

<210> 68

<211> 1587

<212> DNA

<213>Pseudomonas putida (Pseudomonas putida)

<400> 68

atggcttcgg tacacggcac cacatacgaa ctcttgcgac gtcaaggcat cgatacggtc 60

ttcggcaatc ctggctcgaa cgagctcccg tttttgaagg actttccaga ggactttcga 120

tacatcctgg ctttgcagga agcgtgtgtg gtgggcattg cagacggcta tgcgcaagcc 180

agtcggaagc cggctttcat taacctgcat tctgctgctg gtaccggcaa tgctatgggt 240

gcactcagta acgcctggaa ctcacattcc ccgctgatcg tcactgccgg ccagcagacc 300

agggcgatga ttggcgttga agctctgctg accaacgtcg atgccgccaa cctgccacga 360

ccacttgtca aatggagcta cgagcccgca agcgcagcag aagtccctca tgcgatgagc 420

agggctatcc atatggcaag catggcgcca caaggccctg tctatctttc ggtgccatat 480

gacgattggg ataaggatgc tgatcctcag tcccaccacc tttttgatcg ccatgtcagt 540

tcatcagtac gcctgaacga ccaggatctc gatattctgg tgaaagctct caacagcgca 600

tccaacccgg cgatcgtcct gggcccggac gtcgacgcag caaatgcgaa cgcagactgc 660

gtcatgttgg ccgaacgcct caaagctccg gtttgggttg cgccatccgc tccacgctgc 720

ccattcccta cccgtcatcc ttgcttccgt ggattgatgc cagctggcat cgcagcgatt 780

tctcagctgc tcgaaggtca cgatgtggtt ttggtaatcg gcgctccagt gttccgttac 840

caccaatacg acccaggtca atatctcaaa cctggcacgc gattgatttc ggtgacctgc 900

gacccgctcg aagctgcacg cgcgccaatg ggcgatgcga tcgtggcaga cattggtgcg 960

atggctagcg ctcttgccaa cttggttgaa gagagcagcc gccagctccc aactgcagct 1020

ccggaacccg cgaaggttga ccaagacgct ggccgacttc acccagagac agtgttcgac 1080

acactgaacg acatggcccc ggagaatgcg atttacctga acgagtcgac ttcaacgacc 1140

gcccaaatgt ggcagcgcct gaacatgcgc aaccctggta gctactactt ctgtgcagct 1200

ggcggactgg gcttcgccct gcctgcagca attggcgttc aactcgcaga acccgagcga 1260

caagtcatcg ccgtcattgg cgacggatcg gcgaactaca gcattagtgc gttgtggact 1320

gcagctcagt acaacatccc cactatcttc gtgatcatga acaacggcac ctacggtgcg 1380

ttgcgatggt ttgccggcgt tctcgaagca gaaaacgttc ctgggctgga tgtgccaggg 1440

atcgacttcc gcgcactcgc caagggctat ggtgtccaag cgctgaaagc cgacaacctt 1500

gagcagctca agggttcgct acaagaagcg ctttctgcca aaggcccggt acttatcgaa 1560

gtaagcaccg taagcccggt gaagtga 1587

<210> 69

<211> 1323

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 69

atgcaagcct attttgacca gctcgatcgc gttcgttatg aaggctcaaa atcctcaaac 60

ccgttagcat tccgtcacta caatcccgac gaactggtgt tgggtaagcg tatggaagag 120

cacttgcgtt ttgccgcctg ctactggcac accttctgct ggaacggggc ggatatgttt 180

ggtgtggggg cgtttaatcg tccgtggcag cagcctggtg aggcactggc gttggcgaag 240

cgtaaagcag atgtcgcatt tgagtttttc cacaagttac atgtgccatt ttattgcttc 300

cacgatgtgg atgtttcccc tgagggcgcg tcgttaaaag agtacatcaa taattttgcg 360

caaatggttg atgtcctggc aggcaagcaa gaagagagcg gcgtgaagct gctgtgggga 420

acggccaact gctttacaaa ccctcgctac ggcgcgggtg cggcgacgaa cccagatcct 480

gaagtcttca gctgggcggc aacgcaagtt gttacagcga tggaagcaac ccataaattg 540

ggcggtgaaa actatgtcct gtggggcggt cgtgaaggtt acgaaacgct gttaaatacc 600

gacttgcgtc aggagcgtga acaactgggc cgctttatgc agatggtggt tgagcataaa 660

cataaaatcg gtttccaggg cacgttgctt atcgaaccga aaccgcaaga accgaccaaa 720

catcaatatg attacgatgc cgcgacggtc tatggcttcc tgaaacagtt tggtctggaa 780

aaagagatta aactgaacat tgaagctaac cacgcgacgc tggcaggtca ctctttccat 840

catgaaatag ccaccgccat tgcgcttggc ctgttcggtt ctgtcgacgc caaccgtggc 900

gatgcgcaac tgggctggga caccgaccag ttcccgaaca gtgtggaaga gaatgcgctg 960

gtgatgtatg aaattctcaa agcaggcggt ttcaccaccg gtggtctgaa cttcgatgcc 1020

aaagtacgtc gtcaaagtac tgataaatat gatctgtttt acggtcatat cggcgcgatg 1080

gatacgatgg cactggcgct gaaaattgca gcgcgcatga ttgaagatgg cgagctggat 1140

aaacgcatcg cgcagcgtta ttccggctgg aatagcgaat tgggccagca aatcctgaaa 1200

ggccaaatgt cactggcaga tttagccaaa tatgctcagg aacatcattt gtctccggtg 1260

catcagagtg gtcgccagga acaactggaa aatctggtaa accattatct gttcgacaaa 1320

taa 1323

<210> 70

<211> 1455

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 70

atgtatatcg ggatagatct tggcacctcg ggcgtaaaag ttattttgct caacgagcag 60

ggtgaggtgg ttgctgcgca aacggaaaag ctgaccgttt cgcgcccgca tccactctgg 120

tcggaacaag acccggaaca gtggtggcag gcaactgatc gcgcaatgaa agctctgggc 180

gatcagcatt ctctgcagga cgttaaagca ttgggtattg ccggccagat gcacggagca 240

accttgctgg atgctcagca acgggtgtta cgccctgcca ttttgtggaa cgacgggcgc 300

tgtgcgcaag agtgcacttt gctggaagcg cgagttccgc aatcgcgggt gattaccggc 360

aacctgatga tgcccggatt tactgcgcct aaattgctat gggttcagcg gcatgagccg 420

gagatattcc gtcaaatcga caaagtatta ttaccgaaag attacttgcg tctgcgtatg 480

acgggggagt ttgccagcga tatgtctgac gcagctggca ccatgtggct ggatgtcgca 540

aagcgtgact ggagtgacgt catgctgcag gcttgcgact tatctcgtga ccagatgccc 600

gcattatacg aaggcagcga aattactggt gctttgttac ctgaagttgc gaaagcgtgg 660

ggtatggcga cggtgccagt tgtcgcaggc ggtggcgaca atgcagctgg tgcagttggt 720

gtgggaatgg ttgatgctaa tcaggcaatg ttatcgctgg ggacgtcggg ggtctatttt 780

gctgtcagcg aagggttctt aagcaagcca gaaagcgccg tacatagctt ttgccatgcg 840

ctaccgcaac gttggcattt aatgtctgtg atgctgagtg cagcgtcgtg tctggattgg 900

gccgcgaaat taaccggcct gagcaatgtc ccagctttaa tcgctgcagc tcaacaggct 960

gatgaaagtg ccgagccagt ttggtttctg ccttatcttt ccggcgagcg tacgccacac 1020

aataatcccc aggcgaaggg ggttttcttt ggtttgactc atcaacatgg ccccaatgaa 1080

ctggcgcgag cagtgctgga aggcgtgggt tatgcgctgg cagatggcat ggatgtcgtg 1140

catgcctgcg gtattaaacc gcaaagtgtt acgttgattg ggggcggggc gcgtagtgag 1200

tactggcgtc agatgctggc ggatatcagc ggtcagcagc tcgattaccg tacggggggg 1260

gatgtggggc cagcactggg cgcagcaagg ctggcgcaga tcgcggcgaa tccagagaaa 1320

tcgctcattg aattgttgcc gcaactaccg ttagaacagt cgcatctacc agatgcgcag 1380

cgttatgccg cttatcagcc acgacgagaa acgttccgtc gcctctatca gcaacttctg 1440

ccattaatgg cgtaa 1455

<210> 71

<211> 906

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 71

atgaaaaaat tcagcggcat tattccaccg gtatccagca cgtttcatcg tgacggaacc 60

cttgataaaa aggcaatgcg cgaagttgcc gacttcctga ttaataaagg ggtcgacggg 120

ctgttttatc tgggtaccgg tggtgaattt agccaaatga atacagccca gcgcatggca 180

ctcgccgaag aagctgtaac cattgtcgac gggcgagtgc cggtattgat tggcgtcggt 240

tccccttcca ctgacgaagc ggtcaaactg gcgcagcatg cgcaagccta cggcgctgat 300

ggtatcgtcg ccatcaaccc ctactactgg aaagtcgcac cacgaaatct tgacgactat 360

taccagcaga tcgcccgtag cgtcacccta ccggtgatcc tgtacaactt tccggatctg 420

acgggtcagg acttaacccc ggaaaccgtg acgcgtctgg ctctgcaaaa cgagaatatc 480

gttggcatca aagacaccat cgacagcgtt ggtcacttgc gtacgatgat caacacagtt 540

aagtcggtac gcccgtcgtt ttcggtattc tgcggttacg atgatcattt gctgaatacg 600

atgctgctgg gcggcgacgg tgcgataacc gccagcgcta actttgctcc ggaactctcc 660

gtcggcatct accgcgcctg gcgtgaaggc gatctggcga ccgctgcgac gctgaataaa 720

aaactactac aactgcccgc tatttacgcc ctcgaaacac cgtttgtctc actgatcaaa 780

tacagcatgc agtgtgtcgg gctgcctgta gagacatatt gcttaccacc gattcttgaa 840

gcatctgaag aagcaaaaga taaagtccac gtgctgctta ccgcgcaggg cattttacca 900

gtctga 906

<210> 72

<211> 909

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 72

atgccgcagt ccgcgttgtt cacgggaatc attccccctg tctccaccat ttttaccgcc 60

gacggccagc tcgataagcc gggcaccgcc gcgctgatcg acgatctgat caaagcaggc 120

gttgacggcc tgttcttcct gggcagcggt ggcgagttct cccagctcgg cgccgaagag 180

cgtaaagcca ttgcccgctt tgctatcgat catgtcgatc gtcgcgtgcc ggtgctgatc 240

ggcaccggcg gcaccaacgc ccgggaaacc atcgaactca gccagcacgc gcagcaggcg 300

ggcgcggacg gcatcgtggt gatcaacccc tactactgga aagtgtcgga agcgaacctg 360

atccgctatt tcgagcaggt ggccgacagc gtcacgctgc cggtgatgct ctataacttc 420

ccggcgctga ccgggcagga tctgactccg gcgctggtga aaaccctcgc cgactcgcgc 480

agcaatatta tcggcatcaa agacaccatc gactccgtcg cccacctgcg cagcatgatc 540

cataccgtca aaggtgccca tccgcacttc accgtgctct gcggctacga cgatcatctg 600

ttcaataccc tgctgctcgg cggcgacggg gcgatatcgg cgagcggcaa ctttgccccg 660

caggtgtcgg tgaatcttct gaaagcctgg cgcgacgggg acgtggcgaa agcggccggg 720

tatcatcaga ccttgctgca aattccgcag atgtatcagc tggatacgcc gtttgtgaac 780

gtgattaaag aggcgatcgt gctctgcggt cgtcctgtct ccacgcacgt gctgccgccc 840

gcctcgccgc tggacgagcc gcgcaaggcg cagctgaaaa ccctgctgca acagctcaag 900

ctttgctga 909

<210> 73

<211> 975

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 73

atgaagccgt ccgttatcct ctacaaagcc ttacctgatg atttactgca acgcctgcaa 60

gagcatttca ccgttcacca ggtggcaaac ctcagcccac aaaccgtcga acaaaatgca 120

gcaatttttg ccgaagctga aggtttactg ggttcaaacg agaatgtaaa tgccgcattg 180

ctggaaaaaa tgccgaaact gcgtgccaca tcaacgatct ccgtcggcta tgacaatttt 240

gatgtcgatg cgcttaccgc ccgaaaaatt ctgctgatgc acacgccaac cgtattaaca 300

gaaaccgtcg ccgatacgct gatggcgctg gtgttgtcta ccgctcgtcg ggttgtggag 360

gtagcagaac gggtaaaagc aggcgaatgg accgcgagca taggcccgga ctggtacggc 420

actgacgttc accataaaac actgggcatt gtcgggatgg gacggatcgg catggcgctg 480

gcacaacgtg cgcactttgg cttcaacatg cccatcctct ataacgcgcg ccgccaccat 540

aaagaagcag aagaacgctt caacgcccgc tactgcgatt tggatactct gttacaagag 600

tcagatttcg tttgcctgat cctgccgtta actgatgaga cgcatcatct gtttggcgca 660

gaacaattcg ccaaaatgaa atcctccgcc attttcatta atgccggacg tggcccggtg 720

gttgacgaaa atgcactgat cgcagcattg cagaaaggcg aaattcacgc tgccgggctg 780

gatgtcttcg aacaagagcc actgtccgta gattcgccgt tgctctcaat ggccaacgtc 840

gtcgcagtac cgcatattgg atctgccacc catgagacgc gttatggcat ggccgcctgt 900

gccgtggata atttgattga tgcgttacaa ggaaaggttg agaagaactg tgtgaatccg 960

cacgtcgcgg actaa 975

<210> 74

<211> 939

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 74

atggatatca tcttttatca cccaacgttc gatacccaat ggtggattga ggcactgcgc 60

aaagctattc ctcaggcaag agtcagagca tggaaaagcg gagataatga ctctgctgat 120

tatgctttag tctggcatcc tcctgttgaa atgctggcag ggcgcgatct taaagcggtg 180

ttcgcactcg gggccggtgt tgattctatt ttgagcaagc tacaggcaca ccctgaaatg 240

ctgaaccctt ctgttccact ttttcgcctg gaagataccg gtatgggcga gcaaatgcag 300

gaatatgctg tcagtcaggt gctgcattgg tttcgacgtt ttgacgatta tcgcatccag 360

caaaatagtt cgcattggca accgctgcct gaatatcatc gggaagattt taccatcggc 420

attttgggcg caggcgtact gggcagtaaa gttgctcaga gtctgcaaac ctggcgcttt 480

ccgctgcgtt gctggagtcg aacccgtaaa tcgtggcctg gcgtgcaaag ctttgccgga 540

cgggaagaac tgtctgcatt tctgagccaa tgtcgggtat tgattaattt gttaccgaat 600

acccctgaaa ccgtcggcat tattaatcaa caattactcg aaaaattacc ggatggcgcg 660

tatctcctca acctggcgcg tggtgttcat gttgtggaag atgacctgct cgcggcgctg 720

gatagcggca aagttaaagg cgcaatgttg gatgttttta atcgtgaacc cttaccgcct 780

gaaagtccgc tctggcaaca tccacgcgtg acgataacac cacatgtcgc cgcgattacc 840

cgtcccgctg aagctgtgga gtacatttct cgcaccattg cccagctcga aaaaggggag 900

agggtctgcg ggcaagtcga ccgcgcacgc ggctactaa 939

<210> 75

<211> 1434

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 75

atgtttaaga atgcatttgc taacctgcaa aaggtcggta aatcgctgat gctgccggta 60

tccgtactgc ctatcgcagg tattctgctg ggcgtcggtt ccgcgaattt cagctggctg 120

cccgccgttg tatcgcatgt tatggcagaa gcaggcggtt ccgtctttgc aaacatgcca 180

ctgatttttg cgatcggtgt cgccctcggc tttaccaata acgatggcgt atccgcgctg 240

gccgcagttg ttgcctatgg catcatggtt aaaaccatgg ccgtggttgc gccactggta 300

ctgcatttac ctgctgaaga aatcgcctct aaacacctgg cggatactgg cgtactcgga 360

gggattatct ccggtgcgat cgcagcgtac atgtttaacc gtttctaccg tattaagctg 420

cctgagtatc ttggcttctt tgccggtaaa cgctttgtgc cgatcatttc tggcctggct 480

gccatcttta ctggcgttgt gctgtccttc atttggccgc cgattggttc tgcaatccag 540

accttctctc agtgggctgc ttaccagaac ccggtagttg cgtttggcat ttacggtttc 600

atcgaacgtt gcctggtacc gtttggtctg caccacatct ggaacgtacc tttccagatg 660

cagattggtg aatacaccaa cgcagcaggt caggttttcc acggcgacat tccgcgttat 720

atggcgggtg acccgactgc gggtaaactg tctggtggct tcctgttcaa aatgtacggt 780

ctgccagctg ccgcaattgc tatctggcac tctgctaaac cagaaaaccg cgcgaaagtg 840

ggcggtatta tgatctccgc ggcgctgacc tcgttcctga ccggtatcac cgagccgatc 900

gagttctcct tcatgttcgt tgcgccgatc ctgtacatca tccacgcgat tctggcaggc 960

ctggcattcc caatctgtat tcttctgggg atgcgtgacg gtacgtcgtt ctcgcacggt 1020

ctgatcgact tcatcgttct gtctggtaac agcagcaaac tgtggctgtt cccgatcgtc 1080

ggtatcggtt atgcgattgt ttactacacc atcttccgcg tgctgattaa agcactggat 1140

ctgaaaacgc cgggtcgtga agacgcgact gaagatgcaa aagcgacagg taccagcgaa 1200

atggcaccgg ctctggttgc tgcatttggt ggtaaagaaa acattactaa cctcgacgca 1260

tgtattaccc gtctgcgcgt cagcgttgct gatgtgtcta aagtggatca ggccggcctg 1320

aagaaactgg gcgcagcggg cgtagtggtt gctggttctg gtgttcaggc gattttcggt 1380

actaaatccg ataacctgaa aaccgagatg gatgagtaca tccgtaacca ctaa 1434

<210> 76

<211> 510

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 76

atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga taccggaact 60

attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt gccggatgtc 120

gtttttgcgg aaaaaatcgt tggtgatggt attgctatca aaccaacggg taacaaaatg 180

gtcgcgccag tagacggcac cattggtaaa atctttgaaa ccaaccacgc attctctatc 240

gaatctgata gcggcgttga actgttcgtc cacttcggta tcgacaccgt tgaactgaaa 300

ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg cgatactgtc 360

attgaatttg atctgccgct gctggaagag aaagccaagt ctaccctgac tccggttgtt 420

atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt aaccgtgggt 480

gaaaccccgg ttatccgcat caagaagtaa 510

<210> 77

<211> 258

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 77

atgttccagc aagaagttac cattaccgct ccgaacggtc tgcacacccg ccctgctgcc 60

cagtttgtaa aagaagctaa gggcttcact tctgaaatta ctgtgacttc caacggcaaa 120

agcgccagcg cgaaaagcct gtttaaactg cagactctgg gcctgactca aggtaccgtt 180

gtgactatct ccgcagaagg cgaagacgag cagaaagcgg ttgaacatct ggttaaactg 240

atggcggaac tcgagtaa 258

<210> 78

<211> 1254

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 78

atgtactatt taaaaaacac aaacttttgg atgttcggtt tattcttttt cttttacttt 60

tttatcatgg gagcctactt cccgtttttc ccgatttggc tacatgacat caaccatatc 120

agcaaaagtg atacgggtat tatttttgcc gctatttctc tgttctcgct attattccaa 180

ccgctgtttg gtctgctttc tgacaaactc gggctgcgca aatacctgct gtggattatt 240

accggcatgt tagtgatgtt tgcgccgttc tttattttta tcttcgggcc actgttacaa 300

tacaacattt tagtaggatc gattgttggt ggtatttatc taggcttttg ttttaacgcc 360

ggtgcgccag cagtagaggc atttattgag aaagtcagcc gtcgcagtaa tttcgaattt 420

ggtcgcgcgc ggatgtttgg ctgtgttggc tgggcgctgt gtgcctcgat tgtcggcatc 480

atgttcacca tcaataatca gtttgttttc tggctgggct ctggctgtgc actcatcctc 540

gccgttttac tctttttcgc caaaacggat gcgccctctt ctgccacggt tgccaatgcg 600

gtaggtgcca accattcggc atttagcctt aagctggcac tggaactgtt cagacagcca 660

aaactgtggt ttttgtcact gtatgttatt ggcgtttcct gcacctacga tgtttttgac 720

caacagtttg ctaatttctt tacttcgttc tttgctaccg gtgaacaggg tacgcgggta 780

tttggctacg taacgacaat gggcgaatta cttaacgcct cgattatgtt ctttgcgcca 840

ctgatcatta atcgcatcgg tgggaaaaac gccctgctgc tggctggcac tattatgtct 900

gtacgtatta ttggctcatc gttcgccacc tcagcgctgg aagtggttat tctgaaaacg 960

ctgcatatgt ttgaagtacc gttcctgctg gtgggctgct ttaaatatat taccagccag 1020

tttgaagtgc gtttttcagc gacgatttat ctggtctgtt tctgcttctt taagcaactg 1080

gcgatgattt ttatgtctgt actggcgggc aatatgtatg aaagcatcgg tttccagggc 1140

gcttatctgg tgctgggtct ggtggcgctg ggcttcacct taatttccgt gttcacgctt 1200

agcggccccg gcccgctttc cctgctgcgt cgtcaggtga atgaagtcgc ttaa 1254

<210> 79

<211> 1545

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 79

atggatgtca ttaaaaagaa acattggtgg caaagcgacg cgctgaaatg gtcagtgcta 60

ggtctgctcg gcctgctggt gggttacctt gttgttttaa tgtacgcaca aggggaatac 120

ctgttcgcca ttaccacgct gatattgagt tcagcggggc tgtatatttt cgccaatcgt 180

aaagcctacg cctggcgcta tgtttacccg ggaatggctg gaatgggatt attcgtcctc 240

ttccctctgg tctgcaccat cgccattgcc ttcaccaact acagcagcac taaccagctg 300

acttttgaac gtgcgcagga agtgttgtta gatcgctcct ggcaagcagg caaaacctat 360

aactttggtc tttacccggc gggcgatgag tggcaactgg cgctcagcga cggcgaaacc 420

ggcaaaaatt acctctccga cgcttttaaa tttggcggcg agcaaaaact gcaactgaaa 480

gaaacgaccg cccagcccga aggcgaacgc gcgaatctgc gcgtgattac ccagaatcgt 540

caggcgctga gtgacattac cgccattctg ccggatggca acaaagtgat gatgagctcc 600

ctgcgccagt tttctggcac gcagccgctc tacacactcg acggtgacgg cacgttgacg 660

aataatcaga gcggcgtgaa atatcgtccg aataaccaaa ttggctttta ccagtccatt 720

accgccgacg gcaactgggg tgatgaaaag ctaagccccg gttacaccgt gaccaccggc 780

tggaaaaact ttacccgcgt ctttaccgac gaaggcattc agaaaccgtt cctcgccatt 840

ttcgtctgga ccgtggtgtt ctcgctgatc actgtctttt taacggtggc ggtcggcatg 900

gttctggcgt gtctggtgca gtgggaagcg ttgcgcggca aagcggtcta tcgcgtcctg 960

ctgattctgc cctacgcggt gccatcgttc atttcaatct tgattttcaa agggttgttt 1020

aaccagagct tcggtgaaat caacatgatg ttgagcgcgc tgtttggcgt gaagcccgcc 1080

tggttcagcg atccgaccac cgcccgcacg atgctaatta tcgtcaatac ctggctgggt 1140

tatccgtaca tgatgatcct ctgcatgggc ttgctgaaag cgattccgga cgatttgtat 1200

gaagcctcag caatggatgg cgcaggtccg ttccagaact tctttaagat tacgctgccg 1260

ctgctgatta aaccgctgac gccgctgatg atcgccagct tcgcctttaa ctttaacaac 1320

ttcgtgctga ttcaactgtt aaccaacggc ggcccggatc gtcttggcac gaccacgcca 1380

gccggttata ccgacctgct tgttaactac acctaccgca tcgcttttga aggcggcggg 1440

ggtcaggact tcggtctggc ggcagcaatt gccacgctga tcttcctgct ggtgggtgcg 1500

ctggcgatag tgaacctgaa agccacgcga atgaagtttg attaa 1545

<210> 80

<211> 891

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 80

atggcaatgg tccaaccgaa atcgcaaaaa gctcgtttat ttattactca cctgctactg 60

ctacttttta tcgcagcgat tatgttcccg ctgctgatgg tcgtcgctat ctcgctgcgt 120

cagggaaact ttgcgaccgg cagcctgatc ccggagcaaa tctcctggga tcactggaaa 180

ctggcgttag gttttagcgt tgaacaggct gatggtcgca ttacgccacc gccattcccg 240

gtactgctgt ggctgtggaa ctcggtaaag gtcgccggga tttccgcgat tggcattgtg 300

gcgctctcca ccacctgcgc ctacgctttc gcccgtatgc gctttccagg caaagcgacg 360

ctgctgaaag gaatgctgat tttccagatg ttcccggcag tactttcact ggtcgcgttg 420

tatgcgttgt ttgatcgtct gggtgagtac attccattca ttggcctgaa tactcacggc 480

ggcgtaattt tcgcgtatct gggtgggatt gcgctgcatg tctggaccat caaaggctat 540

ttcgaaacca tcgacagttc gctggaagaa gctgctgcgc tggatggtgc gacaccgtgg 600

caggccttcc gccttgtcct gttgccgctg tcagtaccga ttctggcggt ggtattcatc 660

ctgtcgttta tcgctgccat tactgaagtt ccggtcgcgt cgctgttact gcgtgacgta 720

aacagctaca ccctggccgt ggggatgcag caatacctca acccgcaaaa ctacctgtgg 780

ggtgactttg ccgccgctgc cgtgatgtct gcattaccga tcaccatcgt cttcttgctg 840

gctcaacgct ggctggtcaa cggcctgacg gcaggtggtg tgaaaggtta a 891

<210> 81

<211> 6546

<212> DNA

<213>Salmonella typhimurium (Salmonella typhimurium)

<400> 81

atgaatgcaa aagtttgggt tctgggcgac gcggtggtgg acctgctgcc ggagagcgaa 60

gggcgcctgc tgcagtgccc tggaggcgcg ccggctaacg tggcggtagg ggttgcccgc 120

cttggcggca acagcggatt tatcggcgcc gtcggcggtg acccgtttgg ccgctacatg 180

cgtcataccc tgcaacagga gcaggtcgac gtcagccata tgtatctcga cgatcagcac 240

cgcacgtcca ctgtggtcgt cgaccttgac gaccaggggg aacgcacctt tacctttatg 300

gtacgcccca gcgcggacct gttcctggtt gaagaagacc tgccacagtt tgccgccgga 360

cagtggttgc acgtctgctc catcgcgctc agcgccgagc ccagccgtag cactaccttc 420

gcggcgatgg agagcatcag gtctgccggc ggtcgggtca gctttgaccc taatattcgt 480

cccgatctct ggcaggatca ggctttgctg ctagcctgcc tcgatcgcgc tttgcacatg 540

gccaacgtgg taaagctatc ggaagaggag ctggtcttca tcagcagcag taatgattta 600

gcatacggaa tcgccagcgt aacggagcgc tatcagccag aattgctact ggtgacccgg 660

ggcaaagcgg gggtgcttgc cgcgtttcag cagaagttta cccatttcaa cgcccggcct 720

gtggccagcg tggacaccac cggcgcggga gacgcatttg tcgccggact gctcgccagc 780

cttgcggcta acgggatgcc aacggacatg accgcactgg aaccgacact cacgcttgca 840

cagacctgcg gcgccctggc caccacagcc aaaggtgcga tgaccgcctt gccttatcag 900

cgcgatctca accgtcagtt ttaatcctta aagccgcttt gcgcggctca ctttgttgca 960

tgcatcacat ttattaaacc ggtttagcat atttgtttta agaaaaacaa aggtcgggct 1020

taacatagcg cctaaaccgg tttagcaaaa attataattt tccattttta cttttgggat 1080

gccaacagca tgtacagaaa aagcacactt gcgatgctta tcgctttgct aaccagcgct 1140

gcctcagccc atgcgcaaac ggatataagc accattgaag cccgactcaa cgcgctggaa 1200

aaacgcctgc aggaggcaga aaacagggcg caaacggcgg aaaaccgcgc cggggcggcg 1260

gagaaaaaag ttcagcaact caccgcgcag cagcaaaaaa accagaactc gactcaggaa 1320

gtggctcagc gtaccgccag acttgagaaa aaagccgatg acaaaagcgg atttgagttt 1380

cacggttacg cccgctccgg cgtgataatg aatgattccg gcgccagcac caaatccgga 1440

gcctacataa cgccggcagg tgaaaccggc ggagctatcg gccgtctggg aaaccaggcc 1500

gatacctatg ttgaaatgaa tcttgaacat aagcagaccc tggataatgg ggccacgacc 1560

cgctttaagg tgatggtcgc cgacgggcaa acctcttata acgactggac tgcaagcacc 1620

agcgatctga acgttcgtca ggcctttgtc gaattgggta acctgccgac gttcgctggg 1680

ccatttaagg gctccaccct gtgggccggg aaacgtttcg accgcgacaa tttcgatatt 1740

cactggattg actctgatgt cgtgttcctc gccggtaccg gtggtggtat ctatgacgtg 1800

aagtggaacg acggcctgcg gagtaatttc tccctgtacg ggcgtaactt cggcgacatt 1860

gatgattcca gcaacagcgt gcagaactat atcctcacca tgaatcactt cgcaggtccg 1920

ctgcagatga tggtcagcgg tctgcgggcg aaggataacg acgagcgtaa agatagcaac 1980

ggcaatctgg caaaaggcga tgcggcaaac accggcgtgc atgcgctgct cggcctgcat 2040

aacgacagtt tctacggcct gcgcgacggt agcagtaaaa ccgctctgct ttatggtcat 2100

ggtctgggcg cagaggttaa aggtatcgga tctgatggcg cacttcgtcc gggagccgac 2160

acatggcgca ttgccagtta cggcaccacg ccgctcagcg aaaactggtc tgttgccccg 2220

gcaatgctgg cgcaacgcag taaagaccgc tatgccgatg gcgacagcta tcagtgggca 2280

acattcaacc tgcgtctgat tcaggcaatc aatcagaatt tcgctctcgc ctacgaaggc 2340

agctaccagt acatggatct taaacccgaa ggttataacg atcgtcaggc ggtgaacggt 2400

agcttctaca agctcacctt cgccccgaca tttaaggtcg gcagtatcgg tgatttcttc 2460

agtcgcccgg agattcgttt ctatacctcc tggatggact ggagcaaaaa actgaataat 2520

tacgccagcg acgacgccct gggcagtgac ggttttaact cgggcggcga atggtctttc 2580

ggtgtgcaga tggaaacctg gttctgacgc ttacgcctga tgacaggaat agccgggggt 2640

cagagcatct ttgtcacccc ggactcaact aagacgcaga aaaagcgctc ccgtgaacgc 2700

gggacgacaa cataaaaatg tttaagcctt aagagggtac tatggatttt gaacagattt 2760

cctgctcgct gcttccgctt cttggaggca aagaaaatat cgccagcgcc gcgcactgcg 2820

ccacgcgcct gcgcctggtg ctggtcgatg attcgctggc cgaccagcag gccatcggca 2880

aagttgaagg ggtgaagggc tgttttcgta atgccggaca gatgcagatt attttcggca 2940

ccggggtggt aaataaggtc tacgctgcct ttactcaggc ggcgggtatt agcgaatcca 3000

gcaaatcgga agccgccgac atcgcggcaa aaaagctcaa tccgttccag cgcatcgccc 3060

gcctgctatc aaacatcttc gtgccgataa tccctgccat cgtcgcctct ggtctgctga 3120

tgggcctgct gggaatggtc aaaacatacg gctgggttga cccgggcaac gccatctaca 3180

tcatgctgga tatgtgcagc tcggcggcat ttatcattct gccgattctg attggcttta 3240

ccgccgcccg cgaattcggc ggtaatcctt atctcggcgc gacgcttggc ggcattctga 3300

ctcatccagc gctgactaac gcctggggcg tggccgcggg tttccacacc atgaactttt 3360

tcggcttcga aattgccatg atcggctatc agggtacggt gttcccggta ctgctggcag 3420

tatggtttat gagcatcgtt gagaagcagt tgcgtcgcgc aatccccgat gccctggatt 3480

tgatcctgac gccgttcctg acggtgatta tatccggttt tatcgccctg ttgattatcg 3540

gcccggccgg tcgcgcactg ggcgacggta tctcgtttgt cctcagcacc ctgattagcc 3600

acgccggctg gctcgccggg ttactgtttg gcggtctcta ttcagttatc gtcattaccg 3660

gtattcatca cagcttccat gcggttgaag ccgggttgct gggcaatccc tccatcggcg 3720

tcaacttcct gctgccgatt tgggcgatgg ccaacgtcgc tcagggcgga gcctgtctgg 3780

cggtgtggtt caaaaccaaa gatgcaaaaa ttaaagccat tactctgccc tcggcgtttt 3840

ccgccatgct gggcatcacc gaggcggcga tttttggtat taacctgcgc tttgtgaagc 3900

catttattgc ggcgctgatt ggtggtgcgg cgggcggcgc atgggtggta tctgtacacg 3960

tctacatgac cgcggtcggc ttgacagcga tccccggcat ggccatcgtg caggccagtt 4020

cgctgttgaa ctacattatc gggatggtta tcgcctttgg cgtcgccttt acggtctccc 4080

tggttttgaa atacaaaacg gacgctgaat aatgtctctt ccatcacgac tgcctgcgat 4140

tttgcaggcc gtaatgcagg gccagccgcg cgcgctggcc gatagccact atccgcgctg 4200

gcaccatgcg ccggtcaccg ggctgatgaa cgaccccaac ggctttatcg aatttgccgg 4260

acgctatcat ctgttttatc agtggaaccc gctcgcctgc gatcatacgt ttaagtgctg 4320

ggcgcactgg agttccatcg atctgctgca ctggcagcat gagcccattg cgctgatgcc 4380

ggacgaagag tatgaccgta acggctgcta ctccggcagc gcggtggata acaacggtac 4440

gcttaccctg tgctataccg gcaacgtgaa gtttgccgag ggagggcgaa ccgcctggca 4500

atgcctggca acggaaaacg ctgacggcac cttccgcaaa atcggtccgg tcctgccgct 4560

gccggagggc tacaccggcc acgtgcgcga cccaaaagtc tggcgacacg aagacctgtg 4620

gtacatggtg ctgggcgcgc aggatcggca aaagcgcggc aaggtgctgc tgttcagctc 4680

tgcggatctc catcagtgga cgagtatggg tgaaatcgcc ggccacggca tcaatggcct 4740

cgacgacgtc ggctatatgt gggagtgccc ggatcttttt ccactcggcg accagcatat 4800

tctaatctgc tgtccgcagg ggattgcccg tgaggaagag tgctacctga acacctaccc 4860

ggcagtatgg atggcgggcg agtttgatta cgctgctggc gctttcagac acggcgaact 4920

gcacgaactg gacgccgggt ttgagttcta cgccccgcaa accatgctta ccagtgatgg 4980

ccgtcgtctg ctggtcggct ggatgggcgt gccggagggc gaagagatgc ttcagccgac 5040

cctgaacaac ggctggatcc atcagatgac ctgcctgcgt gagctggagt ttatcaacgg 5100

tcagctctat cagcgtccgc tacgggaact gagcgccctg cgcggtgaag cgaacggctg 5160

gtcggggaac gccctgccgc tggcaccgat ggaaatcgat ttgcaaaccc gcgggggcga 5220

tatgttgagc ctcgattttg gcggcgtatt aacccttgag tgcgatgcca gcggactccg 5280

cctggcccga cgcagtctcg ccagtgacga gatgcattat cgttactggc gcggaaacgt 5340

ccgctcgctg cgtgttttca tcgaccagtc gagcgtggag attttcataa acggcggtga 5400

aggggtgatg agcagccgct acttcccggc ctgctccggt cagctaacat tctccggcat 5460

cacgccggac gcattctgct actggccgct gcgaacttgc atggtagaat aagcgttttg 5520

cttcaggctc atggcgtcgt aatgaaaacc aaacgcgtaa ccattaaaga tatagccgaa 5580

caggctggcg tctccaaagc gaccgccagc ttggtactga atggtcgtgg caaggagctg 5640

cgcgtggcgc aggaaacgcg tgagcgcgta ctgtcgattg cccgtaagca tcactatcag 5700

ccaagcattc atgcccgctc gctgcgcaac aaccgcagcc acaccatcgg gctggtggtg 5760

ccggagatca ccaaccacgg ctttgcggtc tttgcccatg agctggagat gctgtgccgc 5820

gaggcgggcg tccagctgtt gatctcttgt actgatgaaa accccggtca ggagagcgtg 5880

gtggtcaata atatgattgc ccgccaggtc gacgggatga tcgtcgcttc ctgtatgcac 5940

aacgatgccg actatctcaa actcagccaa cagctgccag tggtgctgtt tgaccggtgc 6000

cccaatgaaa gcgcgctgcc gctggtaatg accgattcga ttaccccaac ggcggaactg 6060

atttcccgca tcgcgcctca gcatagcgat gagttctggt ttttaggcgg tcaggcgcgt 6120

ctgtcgccct cccgcgatcg tctgaccggg ttcacgcagg gtttggctca ggcgggtatt 6180

gccctgcgcc cggaatgggt gatcaacggc aattaccacc ccagctccgg ctatgagatg 6240

tttgccgcac tctgcgcgcg ccttgggcgg ccgcctaagg cgctattcac cgccgcctgc 6300

gggctgctcg aaggggttct gcgctatatg agccagcacc atttactcga ttccgatatt 6360

catctgacga gctttgacga tcactatctt tatgattcgc tgtcgctgcg tatcgacact 6420

gtccagcagg ataatcgcca gctggcctgg cactgctacg atctgataag ccagctgatc 6480

gagggcgata cgcccgaaac gctacaacgc tacctgcccg caaccctgca gtttcggcat 6540

cagtaa 6546

<210> 82

<211> 4885

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 82

ctatattgct gaaggtacag gcgtttccat aactatttgc tcgcgttttt tactcaggaa 60

gaaaatgcca aatagcaaca tcaggcagac aatacccgaa attgcgaaga aaactgtctg 120

gtagcctgcg tggtcaaaga gtatcccagt cggcgttgaa agcagcacaa tcccaagcga 180

actggcaatt tgaaaaccaa tcagaaagat cgtcgacgac aggcgcttat caaagtttgc 240

cacgctgtat ttgaagacgg atatgacaca aagtggaacc tcaatggcat gtaacagctt 300

cactaatgaa ataatccagg ggttaacgaa caacgcgcag gaaaggatac gcaacgccat 360

aatcacaaca ccgataagta atgcattttt tggccctacc cgattcacaa agaaaggaat 420

aatcgccatg cacagcgctt cgagtaccac ctggaatgag ttgagataac catacaggcg 480

cgttcctaca tcgtgtgatt cgaataaacc tgcataaaag acaggaaaga gttgttgatc 540

aaaaatgtta tagaaagacc acgtccccac aataaatatg acgaaaaccc agaagtttcg 600

atccttgaaa actgcgataa aatcctcttt ttttacccct cccgcatccg ccgctatgca 660

ctggtgatcc ttatctttaa aacgcatgtt gatcatcata aatacagcgc caaatagcga 720

gaccaaccag aagttgatat ggggactgat actaaaaaat ataccggcaa agaacgcgcc 780

aatagcatag ccaaaagatc cccaggcgcg cgctgttcca tattcgaaat gaaaatttcg 840

cgccattttt tcggtgaagc tgtcaagcaa accgcatccc gccagatacc ccaggccaaa 900

aaagagcgcc cccagaatta gacctacaga aaaattgctt tgcagtaacg gttcataaac 960

gtaaatcata aacggtccgg tcaagaccag aatgaaactc atacaccaga tgagcggttt 1020

cttcagaccg agtttatcct gaacgatgcc gtagaacatc ataaatagaa tgctggtaaa 1080

ctggttgacc gaataaagtg tacctaattc cgtccctgtt aatcctagat gtcctttcag 1140

ccaaatagcg tataacgacc accacagcga ccaggaaata aaaaagagaa atgagtaact 1200

ggatgcaaaa cgatagtacg catttctgaa tggaatattc agtgccataa ttacctgcct 1260

gtcgttaaaa aattcatgtc ctatttagag ataagagcgg cctcgccgtt tacttctcac 1320

tttccagttc ttgtcgacat ggcagcgctg tcattgcccc tttcgctgtt actgcaagcg 1380

ctccgcaacg ttgagcgaga tcgataattc gtcgcatttc tctctcatct gtagataatc 1440

ccgtagagga cagacctgtg agtaacccgg caacgaacgc atctcccgcc ccagtgctat 1500

cgacacaatt cacagacatt ccagcaaaat ggtggacttg tcctcgataa cagaccacca 1560

ccccttctgc acctttagtc accaacagca tggcgatctc atactctttt gccagggcgc 1620

atatatcccg atcgttctgt gtttttccac tgataagtcg ccattcttct tccgagagct 1680

tgacgacatc cgccagttgt agcgcctgcc gcaaacacaa gcggagcaaa tgctcgtctt 1740

gccatagatc ttcacgaata ttgggatcga agctgacaaa acctccggca tgccggatcg 1800

ccgtcatcgc agtaaatgcg ctggtacgcg aaggctcggc agacaacgca attgaacaga 1860

gatgtaacca ttcgccatgt cgccagcagg gcaagtctgt cgtctctaaa aaaagatcgg 1920

cactggggcg gaccataaac gtaaatgaac gttctccttg atcgttcaga tcgacaagca 1980

ccgtggatgt ccggtgccat tcatcttgct tcagatacgt gatatcgaca ccctcagtta 2040

gcagcgttct ttgcattaac gcaccaaaag gatcatcacc gacccgacct ataaacccac 2100

ttgttccgcc taatctggcg attcccaccg caacgttagc tggcgcgccg ccaggacaag 2160

gcagtagccg cccgtctgat tctggcaaga gatctacgac cgcatcccct aaaacccata 2220

ctttggctga catttttttc ccttaaattc atctgactta cgcatagtga taaacctctt 2280

tttcgcaaaa tcgtcatgga tttactaaaa catgcatatt cgatcacaaa acgtcatagt 2340

taacgttaac atttgtgata ttcatcgcat ttatgaaagt aagggacttt atttttataa 2400

aagttaacgt taacaattca ccaaatttgc ttaaccagga tgattaaaat gacgcaatct 2460

cgattgcatg cggcgcaaaa cgcactagca aaacttcacg agcgccgagg taacactttc 2520

tatccccatt ttcacctcgc gcctcctgcc gggtggatga acgatccaaa cggcctgatc 2580

tggtttaacg atcgttatca cgcgttttat caacatcacc cgatgagcga acactggggg 2640

ccaatgcact ggggacatgc caccagcgac gatatgatcc actggcagca tgagcctatt 2700

gcgctagcgc caggagacga gaatgacaaa gacgggtgtt tttcaggtag tgctgtcgat 2760

gacaatggtg tcctctcact tatctacacc ggacacgtct ggctcgatgg tgcaggtaat 2820

gacgatgcaa ttcgcgaagt acaatgtctg gctaccagtc gggatggtat tcatttcgag 2880

aaacagggtg tgatcctcac tccaccagaa ggcatcatgc acttccgcga tcctaaagtg 2940

tggcgtgaag ccgacacatg gtggatggta gtcggggcga aagacccagg caacacgggg 3000

cagatcctgc tttatcgcgg cagttcattg cgtgaatgga ctttcgatcg cgtactggcc 3060

cacgctgatg cgggtgaaag ctatatgtgg gaatgtccgg actttttcag ccttggcgat 3120

cagcattatc tgatgttttc cccgcaggga atgaatgccg agggatacag ttatcgaaat 3180

cgctttcaaa gtggcgtaat acccggaatg tggtcgccag gacgactttt tgcacaatcc 3240

gggcatttta ctgaacttga taacgggcat gacttttatg caccacaaag ctttgtagcg 3300

aaggatggtc ggcgtattgt tatcggctgg atggatatgt gggaatcgcc aatgccctca 3360

aaacgtgaag gctgggcagg ctgcatgacg ctggcgcgcg agctatcaga gagcaatggc 3420

aaactcctac aacgcccggt acacgaagct gagtcgttac gccagcagca tcaatctatc 3480

tctccccgca caatcagcaa taaatatgtt ttgcaggaaa acgcgcaagc agttgagatt 3540

cagttgcagt gggcgctgaa gaacagtgat gccgaacatt acggattaca gctcggcgct 3600

ggaatgcggc tgtatattga taaccaatct gagcgacttg ttttgtggcg gtattaccca 3660

cacgagaatt tagatggcta ccgtagtatt cccctcccgc agggtgacat gctcgcccta 3720

aggatattta tcgatacatc atccgtggaa gtatttatta acgacgggga ggcggtgatg 3780

agtagccgaa tatatccgca gccagaagaa cgggaactgt cgctctatgc ctcccacgga 3840

gtggctgtgc tgcaacatgg agcactctgg caactgggtt aacataatat caggtggaac 3900

aacggatcaa cagcgggcaa gggatccgcg tcactcttcc cccttcacga ccttcaataa 3960

tatgcaatgc agcttcccgc ccgataatgt catgtggaag ctgaattgtg gtcagcggcg 4020

gtaaaaacag atgcccgacg ccaaccagat tatcaaagcc cattacggcg acatcctgcg 4080

ggatacgtac ccccttcgcc aaaagaacct gataagccac aaaggctgcg cgatcgttac 4140

cacatatcag aacatcaaaa tctggtttgc ccgatttgaa gtgggcattg agtaaacttg 4200

cgagatcggt gtagtgatca tcacctgttg ccatgtgaaa ttgtttcacc tcagccagat 4260

ctcgtccagc atcacgccag gcctgctcaa atccctgccg acgataccct gttgccaacg 4320

cactttccgg tagccagaag cataacggtt gacgatagcc cgccgcgagc aaatgctgtg 4380

ttgattcata ttgtgcagtg taatcatcag ggatataact gggtaacgct gggtcatccg 4440

ccacacagtt cgccaataca atattttcac catacagaga ctcaggcagc gtgatatgtc 4500

gcagccccat tgtagtatag ataatgccat ccggacggtg ggcaagcagc tgacgtgccg 4560

cgcgggcagc gtcatcttca gaaaaaatat tgattaaaaa actattccag ccgaactcgc 4620

tggcggtttg ctcaatggca agcagaatat caacagagaa aggagtggta gcagtgtcct 4680

gcgccagcac ggcgagagtc gacggcttac gtccttgagc gcgcatctta cgggcggaaa 4740

gatcaggaac ataattcagg gtctggattg cctgcaatac gcggtcacgc gttgcaggac 4800

gcacagattc tgcattatgc atcacccggg agactgtcat catcgacact cccgccaggc 4860

gtgcgacatc ctttaatgaa gccat 4885

<210> 83

<211> 3771

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 83

atgaaaataa agaacattct actcaccctt tgcacctcac tcctgcttac caacgttgct 60

gcacacgcca aagaagtcaa aataggtatg gcgattgatg atctccgtct tgaacgctgg 120

caaaaagatc gagatatctt tgtgaaaaag gcagaatctc tcggcgcgaa agtatttgta 180

cagtctgcaa atggcaatga agaaacacaa atgtcgcaga ttgaaaacat gataaaccgg 240

ggtgtcgatg ttcttgtcat tattccgtat aacggtcagg tattaagtaa cgttgtaaaa 300

gaagccaaac aagaaggcat taaagtatta gcttacgacc gtatgattaa cgatgcggat 360

atcgattttt atatttcttt cgataacgaa aaagtcggtg aactgcaggc aaaagccctg 420

gtcgatattg ttccgcaagg taattacttc ctgatgggcg gctcgccggt agataacaac 480

gccaagctgt tccgcgccgg acaaatgaaa gtgttaaaac cttacgttga ttccggaaaa 540

attaaagtcg ttggtgacca atgggttgat ggctggttac cggaaaacgc attgaaaatt 600

atggaaaacg cgctaaccgc caataataac aaaattgatg ctgtagttgc ctcaaacgat 660

gccaccgcag gtggggcaat tcaggcatta agcgcgcaag gtttatcagg gaaagtagca 720

atctccggcc aggatgcgga tctcgcaggt attaaacgta ttgctgccgg tacgcaaact 780

atgacggtgt ataaacctat tacgttgttg gcaaatactg ccgcagaaat tgccgttgag 840

ttgggcaatg gtcaggaacc aaaagcagat accacactga ataatggcct gaaagatgtc 900

ccctcccgcc tcctgacacc gatcgatgtg aataaaaaca acatcaaaga tacggtaatt 960

aaagacggat tccacaaaga gagcgagctg taagcgttac gccccagcgc ggagcggggg 1020

cgtgatttct ctccatgccg cgtgaatgaa ttggcttagg tggagtcgtt atgccttatc 1080

tacttgaaat gaagaacatt accaaaacct tcggcagtgt gaaggcgatt gataacgtct 1140

gcttgcggtt gaatgctggc gaaatcgtct cactttgtgg ggaaaatggg tctggtaaat 1200

caacgctgat gaaagtgctg tgtggtattt atccccatgg ctcctacgaa ggcgaaatta 1260

tttttgcggg agaagagatt caggcgagtc acatccgcga taccgaacgc aaaggtatcg 1320

ccatcattca tcaggaattg gccctggtga aagaattgac cgtgctggaa aatatcttcc 1380

tgggtaacga aataacccac aatggcatta tggattatga cctgatgacg ctacgctgtc 1440

agaagctgct cgcacaggtc agtttatcca tttcacctga tacccgcgtt ggcgatttag 1500

ggcttgggca acaacaactg gttgaaattg ccaaggcact taataaacag gtgcgcttgt 1560

taattctcga tgaaccgaca gcctcattaa ctgagcagga aacgtcgatt ttactggata 1620

ttattcgcga tctacaacag cacggtatcg cctgtattta tatttcgcac aaactcaacg 1680

aagtcaaagc gatttccgat acgatttgcg ttattcgcga cggacagcac attggtacgc 1740

gtgatgctgc cggaatgagt gaagacgata ttatcaccat gatggtcggg cgagagttaa 1800

ccgcgcttta ccctaatgaa ccacatacca ccggagatga aatattacgt attgaacatc 1860

tgacggcatg gcatccggtt aatcgtcata ttaaacgagt taatgatgtc tcgttttccc 1920

tgaaacgtgg cgaaatattg ggtattgccg gactcgttgg tgccggacgt accgagacca 1980

ttcagtgcct gtttggtgtg tggcccggac aatgggaagg aaaaatttat attgatggca 2040

aacaggtaga tattcgtaac tgtcagcaag ccatcgccca ggggattgcg atggtccccg 2100

aagacagaaa gcgcgacggc atcgttccgg taatggcggt tggtaaaaat attaccctcg 2160

ccgcactcaa taaatttacc ggtggcatta gccagcttga tgacgcggca gagcaaaaat 2220

gtattctgga atcaatccag caactcaaag ttaaaacgtc gtcccccgac cttgctattg 2280

gacgtttgag cggcggcaat cagcaaaaag cgatcctcgc tcgctgtctg ttacttaacc 2340

cgcgcattct cattcttgat gaacccacca ggggtatcga tattggcgcg aaatacgaga 2400

tctacaaatt aattaaccaa ctcgtccagc agggtattgc cgttattgtc atctcttccg 2460

aattacctga agtgctcggc cttagcgatc gtgtactggt gatgcatgaa gggaaactaa 2520

aagccaacct gataaatcat aacctgactc aggagcaggt gatggaagcc gcattgagga 2580

gcgaacatca tgtcgaaaag caatccgtct gaagtgaaat tggccgtacc gacatccggt 2640

ggcttctccg ggctgaaatc actgaatttg caggtcttcg tgatgattgc agctatcatc 2700

gcaatcatgc tgttctttac ctggaccacc gatggtgcct acttaagcgc ccgtaacgtc 2760

tccaacctgt tacgccagac cgcgattacc ggcatcctcg cggtaggaat ggtgttcgtc 2820

ataatttctg ctgaaatcga cctttccgtc ggctcaatga tggggctgtt aggtggcgtc 2880

gcggcgattt gtgacgtctg gttaggctgg cctttgccac ttaccatcat tgtgacgctg 2940

gttctgggac tgcttctcgg tgcctggaac ggatggtggg tcgcgtaccg taaagtccct 3000

tcatttattg tcaccctcgc gggcatgttg gcatttcgcg gcatactcat tggcatcacc 3060

aacggcacga ctgtatcccc caccagcgcc gcgatgtcac aaattgggca aagctatctc 3120

cccgccagta ccggcttcat cattggcgcg cttggcttaa tggcttttgt tggttggcaa 3180

tggcgcggaa gaatgcgccg tcaggctttg ggtttacagt ctccggcctc taccgcagta 3240

gtcggtcgcc aggctttaac cgctatcatc gtattaggcg caatctggct gttgaatgat 3300

taccgtggcg ttcccactcc tgttctgctg ctgacgttgc tgttactcgg cggaatgttt 3360

atggcaacgc ggacggcatt tggacgacgc atttatgcca tcggcggcaa tctggaagca 3420

gcacgtctct ccgggattaa cgttgaacgc accaaacttg ccgtgttcgc gattaacgga 3480

ttaatggtag ccatcgccgg attaatcctt agttctcgac ttggcgctgg ttcaccttct 3540

gcgggaaata tcgccgaact ggacgcaatt gcagcatgcg tgattggcgg caccagcctg 3600

gctggcggtg tgggaagcgt tgccggagca gtaatggggg catttatcat ggcttcactg 3660

gataacggca tgagtatgat ggatgtaccg accttctggc agtatatcgt taaaggtgcg 3720

attctgttgc tggcagtatg gatggactcc gcaaccaaac gccgttcttg a 3771

<210> 84

<211> 1395

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 84

atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt cgtctgcttc 60

cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc tggcgcactg 120

ccgtttattg cagatgaatt ccagattact tcgcacacgc aagaatgggt cgtaagctcc 180

atgatgttcg gtgcggcagt cggtgcggtg ggcagcggct ggctctcctt taaactcggg 240

cgcaaaaaga gcctgatgat cggcgcaatt ttgtttgttg ccggttcgct gttctctgcg 300

gctgcgccaa acgttgaagt actgattctt tcccgcgttc tactggggct ggcggtgggt 360

gtggcctctt ataccgcacc gctgtacctc tctgaaattg cgccggaaaa aattcgtggc 420

agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc ttatctttct 480

gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat tatcatcccg 540

gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg gtttgccgcc 600

aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac cagcgcggaa 660

gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag tggctgggcg 720

ctgtttaaag agaacagcaa cttccgccgc gcggtgttcc ttggcgtact gttgcaggta 780

atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat cttcgaactg 840

gcgggttata ccaacactac cgagcaaatg tgggggaccg tgattgtcgg cctgaccaac 900

gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa accaacgcta 960

acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat gatgcatatc 1020

ggtattcact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat gtttattgtc 1080

ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat tcagccgctg 1140

aaaggccgcg attttggcat cacctgctcc actgccacca actggattgc caacatgatc 1200

gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac cttctgggtg 1260

tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc ggaaaccaaa 1320

cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact gcgcgaaata 1380

ggcgctcacg attaa 1395

<210> 85

<211> 1422

<212> DNA

<213>Zymomonas mobilis (Zymomonas mobilis)

<400> 85

atgagttctg aaagtagtca gggtctagtc acgcgactag ccctaatcgc tgctataggc 60

ggcttgcttt tcggttacga ttcagcggtt atcgctgcaa tcggtacacc ggttgatatc 120

cattttattg cccctcgtca cctgtctgct acggctgcgg cttccctttc tgggatggtc 180

gttgttgctg ttttggtcgg ttgtgttacc ggttctttgc tgtctggctg gattggtatt 240

cgcttcggtc gtcgcggcgg attgttgatg agttccattt gtttcgtcgc cgccggtttt 300

ggtgctgcgt taaccgaaaa attatttgga accggtggtt cggctttaca aattttttgc 360

tttttccggt ttcttgccgg tttaggtatc ggtgtcgttt caaccttgac cccaacctat 420

attgctgaaa ttcgtccgcc agacaaacgt ggtcagatgg tttctggtca gcagatggcc 480

attgtgacgg gtgctttaac cggttatatc tttacctggt tactggctca tttcggttct 540

atcgattggg ttaatgccag tggttggtgc tggtctccgg cttcagaagg cctgatcggt 600

attgccttct tattgctgct gttaaccgca ccggatacgc cgcattggtt ggtgatgaag 660

ggacgtcatt ccgaggctag caaaatcctt gctcgtctgg aaccgcaagc cgatcctaat 720

ctgacgattc aaaagattaa agctggcttt gataaagcca tggacaaaag cagcgcaggt 780

ttgtttgctt ttggtatcac cgttgttttt gccggtgtat ccgttgctgc cttccagcag 840

ttagtcggta ttaacgccgt gctgtattat gcaccgcaga tgttccagaa tttaggtttt 900

ggagctgata cggcattatt gcagaccatc tctatcggtg ttgtgaactt catcttcacc 960

atgattgctt cccgtgttgt tgaccgcttc ggccgtaaac ctctgcttat ttggggtgct 1020

ctcggtatgg ctgcaatgat ggctgtttta ggctgctgtt tctggttcaa agtcggtggt 1080

gttttgcctt tggcttctgt gcttctttat attgcagtct ttggtatgtc atggggccct 1140

gtctgctggg ttgttctgtc agaaatgttc ccgagttcca tcaagggcgc agctatgcct 1200

atcgctgtta ccggacaatg gttagctaat atcttggtta acttcctgtt taaggttgcc 1260

gatggttctc cagcattgaa tcagactttc aaccacggtt tctcctatct cgttttcgca 1320

gcattaagta tcttaggtgg cttgattgtt gctcgcttcg tgccggaaac caaaggtcgg 1380

agcctggatg aaatcgagga gatgtggcgc tcccagaagt ag 1422

<210> 86

<211> 984

<212> DNA

<213>Zymomonas mobilis (Zymomonas mobilis)

<400> 86

atggaaattg ttgcgattga catcggtgga acgcatgcgc gtttctctat tgcggaagta 60

agcaatggtc gggttctttc tcttggagaa gaaacaactt ttaaaacggc agaacatgct 120

agcttgcagt tagcttggga acgtttcggt gaaaaactgg gtcgtcctct gccacgtgcc 180

gcagctattg catgggctgg cccggttcat ggtgaagttt taaaacttac caataaccct 240

tgggtattaa gaccagctac tctgaatgaa aagctggaca tcgatacgca tgttctgatc 300

aatgacttcg gcgcggttgc ccacgcggtt gcgcatatgg attcttctta tctggatcat 360

atttgtggtc ctgatgaagc gcttcctagc gatggtgtta tcactattct tggtccggga 420

acgggcttgg gtgttgccca tctgttgcgg actgaaggcc gttatttcgt catcgaaact 480

gaaggcggtc atatcgactt tgctccgctt gacagacttg aagacaaaat tctggcacgt 540

ttacgtgaac gtttccgccg cgtttctatc gaacgcatta tttctggccc gggtcttggt 600

aatatctacg aagcactggc tgccattgaa ggcgttccgt tcagcttgct ggatgatatt 660

aaattatggc agatggcttt ggaaggtaaa gacaaccttg ctgaagccgc tttggatcgc 720

ttctgcttga gccttggcgc tatcgctggt gatcttgctt tggcacaggg tcgaaccagt 780

gttgttattg gcggtggtgt cggtcttcgt atcgcttccc atttgccaga atctggtttc 840

cgtcagcgct ttgtttcaaa aggacgcttt gaacgcgtca tgtccaagat tccggttaag 900

ttgattactt atccgcagcc tggactgttg ggtgcgcagc tgcctatgcc aacaaatatt 960

ctgaagttga ataatatttt ttaa 984

<210> 87

<211> 966

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 87

atgacaaagt atgcattagt cggtgatgtg ggcggcacca acgcacgtct tgctctgtgt 60

gatattgcca gtggtgaaat ctcgcaggct aagacctatt cagggcttga ttaccccagc 120

ctcgaagcgg tcattcgcgt ttatcttgaa gaacataagg tcgaggtgaa agacggctgt 180

attgccatcg cttgcccaat taccggtgac tgggtggcga tgaccaacca tacctgggcg 240

ttctcaattg ccgaaatgaa aaagaatctc ggttttagcc atctggaaat tattaacgat 300

tttaccgctg tatcgatggc gatcccgatg ctgaaaaaag agcatctgat tcagtttggt 360

ggcgcagaac cggtcgaagg taagcctatt gcggtttacg gtgccggaac ggggcttggg 420

gttgcgcatc tggtccatgt cgataagcgt tgggtaagct tgccaggcga aggcggtcac 480

gttgattttg cgccgaatag tgaagaagag gccattatcc tcgaaatatt gcgtgcggaa 540

attggtcatg tttcggcgga gcgcgtgctt tctggccctg ggctggtgaa tttgtatcgc 600

gcaattgtga aagctgacaa ccgcctgcca gaaaatctca agccaaaaga tattaccgaa 660

cgcgcgctgg ctgacagctg caccgattgc cgccgcgcat tgtcgctgtt ttgcgtcatt 720

atgggccgtt ttggcggcaa tctggcgctc aatctcggga catttggcgg cgtgtttatt 780

gcgggcggta tcgtgccgcg cttccttgag ttcttcaaag cctccggttt ccgtgccgca 840

tttgaagata aagggcgctt taaagaatat gtccatgata ttccggtgta tctcatcgtc 900

catgacaatc cgggccttct cggttccggt gcacatttac gccagacctt aggtcacatt 960

ctgtaa 966

<210> 88

<211> 2547

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 88

ttgtacctct atattgagac tctgaaacag agactggatg ccataaatca attgcgtgtg 60

gatcgcgcgc ttgctgctat ggggcctgca ttccaacagg tctacagtct actgccgaca 120

ttgttgcact atcaccatcc gctaatgccg ggttaccttg atggtaacgt tcccaaaggc 180

atttgccttt acacgcctga tgaaactcaa cgccactacc tgaacgagct tgaactgtat 240

cgtggaatgt cagtacagga tccgccgaaa ggtgagcttc caattactgg tgtatacacc 300

atgggcagca cctcgtccgt agggcaaagt tgttcctctg acctggatat ctgggtctgt 360

catcaatcct ggctcgatag cgaagagcgc caattgctac aacgtaaatg tagcctgctg 420

gaaaactggg ccgcctcgct gggtgtggaa gtcagcttct tcctgattga tgaaaaccgc 480

ttccgtcata atgaaagcgg cagcctgggg ggcgaagatt gtggctccac ccagcatata 540

ctgctgcttg acgaatttta tcgtaccgcc gtgcgtctcg ccggtaagcg tattctgtgg 600

aatatggtgc cgtgcgacga agaagagcat tacgacgact atgtgatgac gctttacgcg 660

cagggcgtgc tgacgccaaa tgaatggctg gatctcggtg gcttaagctc gctttctgct 720

gaagagtact ttggtgccag cctttggcag ctctacaaga gtatcgattc cccatacaaa 780

gcggtactga aaacactgct gctggaagcc tattcctggg aatacccgaa cccacgtctg 840

ctggcgaaag atatcaaaca gcgtttgcac gacggcgaga ttgtatcgtt tggtctcgat 900

ccatactgca tgatgctgga gcgtgttact gaatacctga cggcgattga agattttacc 960

cgtctggatt tagtacgtcg ctgcttctat ttaaaagtgt gcgaaaagct cagccgtgaa 1020

cgcgcctgcg taggctggcg tcgcgcagtg ttgagccagt tagtgagcga gtggggttgg 1080

gacgaagctc gtctggcaat gctcgataac cgcgctaact ggaagattga tcaggtgcgt 1140

gaggcgcaca acgagttgct cgacgcgatg atgcagagct accgtaatct gatccgcttt 1200

gcgcgtcgca ataaccttag cgtctccgcc agtccgcagg atatcggcgt gctgacgcgt 1260

aagctgtatg ccgcgtttga agcattacca ggtaaagtga cgctggtaaa cccgcagatt 1320

tcacccgatc tctcggaacc gaatctgacc tttatttatg tgccgccggg ccgggctaac 1380

cgttcaggtt ggtatctgta taaccgcgcg ccaaatattg agtcgatcat cagccatcag 1440

ccgctggaat ataaccgtta cctgaataaa ctggtggcgt gggcatggtt taacggcctg 1500

ctgacctcgc gcacccgttt gtatattaaa ggtaacggca ttgtcgattt gcctaagttg 1560

caggagatgg tcgccgacgt gtcgcaccat ttcccgctgc gcttacctgc accgacaccg 1620

aaggcgctct acagcccgtg tgagatccgc catctggcga ttatcgttaa cctggaatat 1680

gacccgacag cggcgttccg caatcaggtg gtgcatttcg atttccgtaa gctggatgtc 1740

ttcagctttg gcgagaatca aaattgcctg gtaggtagcg ttgacctgct gtaccgcaac 1800

tcgtggaacg aagtgcgtac gctgcacttc aacggcgagc aatcgatgat cgaagccctg 1860

aaaactattc tcggcaaaat gcatcaggac gccgcaccgc cagatagcgt ggaagtcttc 1920

tgttatagcc agcatctgcg cggcttaatt cgtactcgcg tgcagcaact ggtttctgag 1980

tgtattgaat tgcgtctttc cagcacccgc caggaaaccg ggcgtttcaa ggcgctgcgc 2040

gtttctggtc aaacctgggg gttgttcttc gaacgcctga atgtatcggt acagaaactg 2100

gaaaacgcca tcgagtttta tggcgcgatt tcgcataaca aactgcacgg cctgtcagtg 2160

caggttgaaa ccaatcacgt caaattaccg gcggtggtgg acggctttgc cagcgaaggg 2220

atcatccagt tctttttcga agaaacgcaa gacgagaatg gctttaatat ctacattctc 2280

gacgaaagca accgggttga ggtatatcac cactgcgaag gcagcaaaga ggagctggta 2340

cgtgacgtca gtcgcttcta ctcgtcatcg catgaccgtt ttacctacgg ctcaagcttc 2400

atcaacttca acctgccgca gttctatcag attgtgaagg ttgatggtcg tgaacaggtg 2460

attccgttcc gcacaaaatc tatcggtaac atgccgcctg ccaatcagga tcacgatacg 2520

ccgctattac agcaatattt ttcgtga 2547

<210> 89

<211> 210

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 89

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys Asp Glu Glu Gly Lys Glu Met Ile Leu Ser Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Gly Arg Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 90

<211> 210

<212> PRT

<213>Artificial

<220>

<223>Crp* mutant

<400> 90

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys His Glu Glu Gly Lys Glu Met Ile Leu Ser Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Gly Arg Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 91

<211> 210

<212> PRT

<213>Artificial

<220>

<223>Crp* mutant

<400> 91

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys Asp Glu Glu Gly Lys Glu Met Ile Leu Phe Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Gly Arg Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 92

<211> 210

<212> PRT

<213>Artificial

<220>

<223>Crp* mutant

<400> 92

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys Asp Glu Glu Gly Lys Glu Met Ile Leu Ser Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Asp Arg Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 93

<211> 210

<212> PRT

<213>Artificial

<220>

<223>Crp* mutant

<400> 93

Met Val Leu Gly Lys Pro Gln Thr Asp Pro Thr Leu Glu Trp Phe Leu

1 5 10 15

Ser His Cys His Ile His Lys Tyr Pro Ser Lys Ser Thr Leu Ile His

20 25 30

Gln Gly Glu Lys Ala Glu Thr Leu Tyr Tyr Ile Val Lys Gly Ser Val

35 40 45

Ala Val Leu Ile Lys Asp Glu Glu Gly Lys Glu Met Ile Leu Ser Tyr

50 55 60

Leu Asn Gln Gly Asp Phe Ile Gly Glu Leu Gly Leu Phe Glu Glu Gly

65 70 75 80

Gln Glu Arg Ser Ala Trp Val Arg Ala Lys Thr Ala Cys Glu Val Ala

85 90 95

Glu Ile Ser Tyr Lys Lys Phe Arg Gln Leu Ile Gln Val Asn Pro Asp

100 105 110

Ile Leu Met Arg Leu Ser Ala Gln Met Ala Arg Arg Leu Gln Val Thr

115 120 125

Ser Glu Lys Val Gly Asn Leu Ala Phe Leu Asp Val Thr Gly Asp Ile

130 135 140

Ala Gln Thr Leu Leu Asn Leu Ala Lys Gln Pro Asp Ala Met Thr His

145 150 155 160

Pro Asp Gly Met Gln Ile Lys Ile Thr Arg Gln Glu Ile Gly Gln Ile

165 170 175

Val Gly Cys Ser Arg Glu Thr Val Gly Arg Ile Leu Lys Met Leu Glu

180 185 190

Asp Gln Asn Leu Ile Ser Ala His Gly Lys Thr Ile Val Val Tyr Gly

195 200 205

Thr Arg

210

<210> 94

<211> 2932

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 94

atgcgaattg gcataccaag agaacggtta accaatgaaa cccgtgttgc agcaacgcca 60

aaaacagtgg aacagctgct gaaactgggt tttaccgtcg cggtagagag cggcgcgggt 120

caactggcaa gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat tgtagaaggg 180

aatagcgtct ggcagtcaga gatcattctg aaggtcaatg cgccgttaga tgatgaaatt 240

gcgttactga atcctgggac aacgctggtg agttttatct ggcctgcgca gaatccggaa 300

ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg cgatggactc tgtgccgcgt 360

atctcacgcg cacaatcgct ggacgcacta agctcgatgg cgaacatcgc cggttatcgc 420

gccattgttg aagcggcaca tgaatttggg cgcttcttta ccgggcaaat tactgcggcc 480

gggaaagtgc caccggcaaa agtgatggtg attggtgcgg gtgttgcagg tctggccgcc 540

attggcgcag caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg cccggaagtg 600

aaagaacaag ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa agaggaagct 660

ggcagcggcg atggctatgc caaagtgatg tcggacgcgt tcatcaaagc ggaaatggaa 720

ctctttgccg cccaggcaaa agaggtcgat atcattgtca ccaccgcgct tattccaggc 780

aaaccagcgc cgaagctaat tacccgtgaa atggttgact ccatgaaggc gggcagtgtg 840

attgtcgacc tggcagccca aaacggcggc aactgtgaat acaccgtgcc gggtgaaatc 900

ttcactacgg aaaatggtgt caaagtgatt ggttataccg atcttccggg ccgtctgccg 960

acgcaatcct cacagcttta cggcacaaac ctcgttaatc tgctgaaact gttgtgcaaa 1020

gagaaagacg gcaatatcac tgttgatttt gatgatgtgg tgattcgcgg cgtgaccgtg 1080

atccgtgcgg gcgaaattac ctggccggca ccgccgattc aggtatcagc tcagccgcag 1140

gcggcacaaa aagcggcacc ggaagtgaaa actgaggaaa aatgtacctg ctcaccgtgg 1200

cgtaaatacg cgttgatggc gctggcaatc attctttttg gctggatggc aagcgttgcg 1260

ccgaaagaat tccttgggca cttcaccgtt ttcgcgctgg cctgcgttgt cggttattac 1320

gtggtgtgga atgtatcgca cgcgctgcat acaccgttga tgtcggtcac caacgcgatt 1380

tcagggatta ttgttgtcgg agcactgttg cagattggcc agggcggctg ggttagcttc 1440

cttagtttta tcgcggtgct tatagccagc attaatattt tcggtggctt caccgtgact 1500

cagcgcatgc tgaaaatgtt ccgcaaaaat taaggggtaa catatgtctg gaggattagt 1560

tacagctgca tacattgttg ccgcgatcct gtttatcttc agtctggccg gtctttcgaa 1620

acatgaaacg tctcgccagg gtaacaactt cggtatcgcc gggatggcga ttgcgttaat 1680

cgcaaccatt tttggaccgg atacgggtaa tgttggctgg atcttgctgg cgatggtcat 1740

tggtggggca attggtatcc gtctggcgaa gaaagttgaa atgaccgaaa tgccagaact 1800

ggtggcgatc ctgcatagct tcgtgggtct ggcggcagtg ctggttggct ttaacagcta 1860

tctgcatcat gacgcgggaa tggcaccgat tctggtcaat attcacctga cggaagtgtt 1920

cctcggtatc ttcatcgggg cggtaacgtt cacgggttcg gtggtggcgt tcggcaaact 1980

gtgtggcaag atttcgtcta aaccattgat gctgccaaac cgtcacaaaa tgaacctggc 2040

ggctctggtc gtttccttcc tgctgctgat tgtatttgtt cgcacggaca gcgtcggcct 2100

gcaagtgctg gcattgctga taatgaccgc aattgcgctg gtattcggct ggcatttagt 2160

cgcctccatc ggtggtgcag atatgccagt ggtggtgtcg atgctgaact cgtactccgg 2220

ctgggcggct gcggctgcgg gctttatgct cagcaacgac ctgctgattg tgaccggtgc 2280

gctggtcggt tcttcggggg ctatcctttc ttacattatg tgtaaggcga tgaaccgttc 2340

ctttatcagc gttattgcgg gtggtttcgg caccgacggc tcttctactg gcgatgatca 2400

ggaagtgggt gagcaccgcg aaatcaccgc agaagagaca gcggaactgc tgaaaaactc 2460

ccattcagtg atcattactc cggggtacgg catggcagtc gcgcaggcgc aatatcctgt 2520

cgctgaaatt actgagaaat tgcgcgctcg tggtattaat gtgcgtttcg gtatccaccc 2580

ggtcgcgggg cgtttgcctg gacatatgaa cgtattgctg gctgaagcaa aagtaccgta 2640

tgacatcgtg ctggaaatgg acgagatcaa tgatgacttt gctgataccg ataccgtact 2700

ggtgattggt gctaacgata cggttaaccc ggcggcgcag gatgatccga agagtccgat 2760

tgctggtatg cctgtgctgg aagtgtggaa agcgcagaac gtgattgtct ttaaacgttc 2820

gatgaacact ggctatgctg gtgtgcaaaa cccgctgttc ttcaaggaaa acacccacat 2880

gctgtttggt gacgccaaag ccagcgtgga tgcaatcctg aaagctctgt aa 2932

<210> 95

<211> 1401

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 95

atgccacatt cctacgatta cgatgccata gtaataggtt ccggccccgg cggcgaaggc 60

gctgcaatgg gcctggttaa gcaaggtgcg cgcgtcgcag ttatcgagcg ttatcaaaat 120

gttggcggcg gttgcaccca ctggggcacc atcccgtcga aagctctccg tcacgccgtc 180

agccgcatta tagaattcaa tcaaaaccca ctttacagcg accattcccg actgctccgc 240

tcttcttttg ccgatatcct taaccatgcc gataacgtga ttaatcaaca aacgcgcatg 300

cgtcagggat tttacgaacg taatcactgt gaaatattgc agggaaacgc tcgctttgtt 360

gacgagcata cgttggcgct ggattgcccg gacggcagcg ttgaaacact aaccgctgaa 420

aaatttgtta ttgcctgcgg ctctcgtcca tatcatccaa cagatgttga tttcacccat 480

ccacgcattt acgacagcga ctcaattctc agcatgcacc acgaaccgcg ccatgtactt 540

atctatggtg ctggagtgat cggctgtgaa tatgcgtcga tcttccgcgg tatggatgta 600

aaagtggatc tgatcaacac ccgcgatcgc ctgctggcat ttctcgatca agagatgtca 660

gattctctct cctatcactt ctggaacagt ggcgtagtga ttcgtcacaa cgaagagtac 720

gagaagatcg aaggctgtga cgatggtgtg atcatgcatc tgaagtcggg taaaaaactg 780

aaagctgact gcctgctcta tgccaacggt cgcaccggta ataccgattc gctggcgtta 840

cagaacattg ggctagaaac tgacagccgc ggacagctga aggtcaacag catgtatcag 900

accgcacagc cacacgttta cgcggtgggc gacgtgattg gttatccgag cctggcgtcg 960

gcggcctatg accaggggcg cattgccgcg caggcgctgg taaaaggcga agccaccgca 1020

catctgattg aagatatccc taccggtatt tacaccatcc cggaaatcag ctctgtgggc 1080

aaaaccgaac agcagctgac cgcaatgaaa gtgccatatg aagtgggccg cgcccagttt 1140

aaacatctgg cacgcgcaca aatcgtcggc atgaacgtgg gcacgctgaa aattttgttc 1200

catcgggaaa caaaagagat tctgggtatt cactgctttg gcgagcgcgc tgccgaaatt 1260

attcatatcg gtcaggcgat tatggaacag aaaggtggcg gcaacactat tgagtacttc 1320

gtcaacacca cctttaacta cccgacgatg gcggaagcct atcgggtagc tgcgttaaac 1380

ggtttaaacc gcctgtttta a 1401

<210> 96

<211> 1434

<212> DNA

<213>Streptococcus mutans (Streptococcus mutans)

<400> 96

atggcaatga caaaacaata taaaaattat gtcaatggcg agtggaagct ttcagaaaat 60

gaaattaaaa tctacgaacc agccagtgga gctgaattgg gttcagttcc agcaatgagt 120

actgaagaag tagattatgt ttatgcttca gccaagaaag ctcaaccagc ttggcgagca 180

ctttcataca tagaacgtgc tgcctacctt cataaggtag cagatatttt gatgcgtgat 240

aaagaaaaaa taggtgctat tctttccaaa gaggttgcta aaggttataa atcagcagtc 300

agcgaagttg ttcgtactgc agaaatcatt aattatgcag ctgaagaagg tcttcgtatg 360

gaaggtgaag tccttgaagg cggcagtttt gaagcagcca gcaagaaaaa aattgccgtt 420

gttcgtcgtg aaccagtagg tcttgtatta gctatttcac catttaacta ccctgttaac 480

ttggcaggtt cgaaaattgc accggctctt attgcgggaa atgttattgc ttttaaacca 540

ccgacgcaag gatcaatctc agggctctta cttgctgaag catttgctga agctggactt 600

cctgcaggtg tctttaatac cattacaggt cgtggttctg aaattggaga ctatattgta 660

gaacatcaag ccgttaactt tatcaatttc actggttcaa caggaattgg cgaacgtatt 720

ggcaaaatgg ctggtatgcg tccgattatg cttgaactcg gtggaaaaga ttcagccatc 780

gttcttgaag atgcggacct tgaattgact gctaaaaata ttattgcagg tgcttttggt 840

tattcaggtc aacgctgtac agcagttaaa cgtgttcttg tgatggaaag tgttgctgat 900

gaactggtcg aaaaaatccg tgaaaaagtt cttgcattaa caattggtaa tccagaagac 960

gatgcagata ttacaccgtt gattgataca aaatcagctg attatgtaga aggtcttatt 1020

aatgatgcca atgataaagg agccactgcc cttactgaaa tcaaacgtga aggtaatctt 1080

atctgtccaa tcctctttga taaggtaacg acagatatgc gtcttgcttg ggaagaacca 1140

tttggtcctg ttcttccgat cattcgtgtg acatctgtag aagaagccat tgaaatttct 1200

aacaaatcgg aatatggact tcaggcttct atctttacaa atgatttccc acgcgctttt 1260

ggtattgctg agcagcttga agttggtaca gttcatatca ataataagac acagcgcggc 1320

acggacaact tcccattctt aggggctaaa aaatcaggtg caggtattca aggggtaaaa 1380

tattctattg aagctatgac aactgttaaa tccgtcgtat ttgatatcaa ataa 1434

<210> 97

<211> 996

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 97

atgactatca aagtaggtat caacggtttt ggccgtatcg gtcgcattgt tttccgtgct 60

gctcagaaac gttctgacat cgagatcgtt gcaatcaacg acctgttaga cgctgattac 120

atggcataca tgctgaaata tgactccact cacggccgtt tcgacggtac cgttgaagtg 180

aaagacggtc atctgatcgt taacggtaaa aaaatccgtg ttaccgctga acgtgatccg 240

gctaacctga aatgggacga agttggtgtt gacgttgtcg ctgaagcaac tggtctgttc 300

ctgactgacg aaactgctcg taaacacatc accgctggtg cgaagaaagt ggttatgact 360

ggtccgtcta aagacaacac tccgatgttc gttaaaggcg ctaacttcga caaatatgct 420

ggccaggaca tcgtttccaa cgcttcctgc accaccaact gcctggctcc gctggctaaa 480

gttatcaacg ataacttcgg catcatcgaa ggtctgatga ccaccgttca cgctactacc 540

gctactcaga aaaccgttga tggcccgtct cacaaagact ggcgcggcgg ccgcggcgct 600

tcccagaaca tcatcccgtc ctctaccggt gctgctaaag ctgtaggtaa agtactgcca 660

gaactgaatg gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa cgtatctgta 720

gttgacctga ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa agctgccgtt 780

aaagctgctg ctgaaggcga aatgaaaggc gttctgggct acaccgaaga tgacgtagta 840

tctaccgatt tcaacggcga agtttgcact tccgtgttcg atgctaaagc tggtatcgct 900

ctgaacgaca acttcgtgaa actggtatcc tggtacgaca acgaaaccgg ttactccaac 960

aaagttctgg acctgatcgc tcacatctcc aaataa 996

<210> 98

<211> 1650

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 98

atgaaaaaca tcaatccaac gcagaccgct gcctggcagg cactacagaa acacttcgat 60

gaaatgaaag acgttacgat cgccgatctt tttgctaaag acggcgatcg tttttctaag 120

ttctccgcaa ccttcgacga tcagatgctg gtggattact ccaaaaaccg catcactgaa 180

gagacgctgg cgaaattaca ggatctggcg aaagagtgcg atctggcggg cgcgattaag 240

tcgatgttct ctggcgagaa gatcaaccgc actgaaaacc gcgccgtgct gcacgtagcg 300

ctgcgtaacc gtagcaatac cccgattttg gttgatggca aagacgtaat gccggaagtc 360

aacgcggtgc tggagaagat gaaaaccttc tcagaagcga ttatttccgg tgagtggaaa 420

ggttataccg gcaaagcaat cactgacgta gtgaacatcg ggatcggcgg ttctgacctc 480

ggcccataca tggtgaccga agctctgcgt ccgtacaaaa accacctgaa catgcacttt 540

gtttctaacg tcgatgggac tcacatcgcg gaagtgctga aaaaagtaaa cccggaaacc 600

acgctgttct tggtagcatc taaaaccttc accactcagg aaactatgac caacgcccat 660

agcgcgcgtg actggttcct gaaagcggca ggtgatgaaa aacacgttgc aaaacacttt 720

gcggcgcttt ccaccaatgc caaagccgtt ggcgagtttg gtattgatac tgccaacatg 780

ttcgagttct gggactgggt tggcggccgt tactctttgt ggtcagcgat tggcctgtcg 840

attgttctct ccatcggctt tgataacttc gttgaactgc tttccggcgc acacgcgatg 900

gacaagcatt tctccaccac gcctgccgag aaaaacctgc ctgtactgct ggcgctgatt 960

ggcatctggt acaacaattt ctttggtgcg gaaactgaag cgattctgcc gtatgaccag 1020

tatatgcacc gtttcgcggc gtacttccag cagggcaata tggagtccaa cggtaagtat 1080

gttgaccgta acggtaacgt tgtggattac cagactggcc cgattatctg gggtgaacca 1140

ggcactaacg gtcagcacgc gttctaccag ctgatccacc agggaaccaa aatggtaccg 1200

tgcgatttca tcgctccggc tatcacccat aacccgctct ctgatcatca ccagaaactg 1260

ctgtctaact tcttcgccca gaccgaagcg ctggcgtttg gtaaatcccg cgaagtggtt 1320

gagcaggaat atcgtgatca gggtaaagat ccggcaacgc ttgactacgt ggtgccgttc 1380

aaagtattcg aaggtaaccg cccgaccaac tccatcctgc tgcgtgaaat cactccgttc 1440

agcctgggtg cgttgattgc gctgtatgag cacaaaatct ttactcaggg cgtgatcctg 1500

aacatcttca ccttcgacca gtggggcgtg gaactgggta aacagctggc gaaccgtatt 1560

ctgccagagc tgaaagatga taaagaaatc agcagccacg atagctcgac caatggtctg 1620

attaaccgct ataaagcgtg gcgcggttaa 1650

<210> 99

<211> 963

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 99

atgattaaga aaatcggtgt gttgacaagc ggcggtgatg cgccaggcat gaacgccgca 60

attcgcgggg ttgttcgttc tgcgctgaca gaaggtctgg aagtaatggg tatttatgac 120

ggctatctgg gtctgtatga agaccgtatg gtacagctag accgttacag cgtgtctgac 180

atgatcaacc gtggcggtac gttcctcggt tctgcgcgtt tcccggaatt ccgcgacgag 240

aacatccgcg ccgtggctat cgaaaacctg aaaaaacgtg gtatcgacgc gctggtggtt 300

atcggcggtg acggttccta catgggtgca atgcgtctga ccgaaatggg cttcccgtgc 360

atcggtctgc cgggcactat cgacaacgac atcaaaggca ctgactacac tatcggtttc 420

ttcactgcgc tgagcaccgt tgtagaagcg atcgaccgtc tgcgtgacac ctcttcttct 480

caccagcgta tttccgtggt ggaagtgatg ggccgttatt gtggagatct gacgttggct 540

gcggccattg ccggtggctg tgaattcgtt gtggttccgg aagttgaatt cagccgtgaa 600

gacctggtaa acgaaatcaa agcgggtatc gcgaaaggta aaaaacacgc gatcgtggcg 660

attaccgaac atatgtgtga tgttgacgaa ctggcgcatt tcatcgagaa agaaaccggt 720

cgtgaaaccc gcgcaactgt gctgggccac atccagcgcg gtggttctcc ggtgccttac 780

gaccgtattc tggcttcccg tatgggcgct tacgctatcg atctgctgct ggcaggttac 840

ggcggtcgtt gtgtaggtat ccagaacgaa cagctggttc accacgacat catcgacgct 900

atcgaaaaca tgaagcgtcc gttcaaaggt gactggctgg actgcgcgaa aaaactgtat 960

taa 963

<210> 100

<211> 1476

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 100

atggcggtaa cgcaaacagc ccaggcctgt gacctggtca ttttcggcgc gaaaggcgac 60

cttgcgcgtc gtaaattgct gccttccctg tatcaactgg aaaaagccgg tcagctcaac 120

ccggacaccc ggattatcgg cgtagggcgt gctgactggg ataaagcggc atataccaaa 180

gttgtccgcg aggcgctcga aactttcatg aaagaaacca ttgatgaagg tttatgggac 240

accctgagtg cacgtctgga tttttgtaat ctcgatgtca atgacactgc tgcattcagc 300

cgtctcggcg cgatgctgga tcaaaaaaat cgtatcacca ttaactactt tgccatgccg 360

cccagcactt ttggcgcaat ttgcaaaggg cttggcgagg caaaactgaa tgctaaaccg 420

gcacgcgtag tcatggagaa accgctgggg acgtcgctgg cgacctcgca ggaaatcaat 480

gatcaggttg gcgaatactt cgaggagtgc caggtttacc gtatcgacca ctatcttggt 540

aaagaaacgg tgctgaacct gttggcgctg cgttttgcta actccctgtt tgtgaataac 600

tgggacaatc gcaccattga tcatgttgag attaccgtgg cagaagaagt ggggatcgaa 660

gggcgctggg gctattttga taaagccggt cagatgcgcg acatgatcca gaaccacctg 720

ctgcaaattc tttgcatgat tgcgatgtct ccgccgtctg acctgagcgc agacagcatc 780

cgcgatgaaa aagtgaaagt actgaagtct ctgcgccgca tcgaccgctc caacgtacgc 840

gaaaaaaccg tacgcgggca atatactgcg ggcttcgccc agggcaaaaa agtgccggga 900

tatctggaag aagagggcgc gaacaagagc agcaatacag aaactttcgt ggcgatccgc 960

gtcgacattg ataactggcg ctgggccggt gtgccattct acctgcgtac tggtaaacgt 1020

ctgccgacca aatgttctga agtcgtggtc tatttcaaaa cacctgaact gaatctgttt 1080

aaagaatcgt ggcaggatct gccgcagaat aaactgacta tccgtctgca acctgatgaa 1140

ggcgtggata tccaggtact gaataaagtt cctggccttg accacaaaca taacctgcaa 1200

atcaccaagc tggatctgag ctattcagaa acctttaatc agacgcatct ggcggatgcc 1260

tatgaacgtt tgctgctgga aaccatgcgt ggtattcagg cactgtttgt acgtcgcgac 1320

gaagtggaag aagcctggaa atgggtagac tccattactg aggcgtgggc gatggacaat 1380

gatgcgccga aaccgtatca ggccggaacc tggggacccg ttgcctcggt ggcgatgatt 1440

acccgtgatg gtcgttcctg gaatgagttt gagtaa 1476

<210> 101

<211> 474

<212> PRT

<213>Escherichia coli (Escherichia coli)

<400> 101

Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro

1 5 10 15

Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr

20 25 30

Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val

35 40 45

Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu

50 55 60

Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys

65 70 75 80

Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln

85 90 95

Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val

100 105 110

Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu

115 120 125

Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala

130 135 140

Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro

145 150 155 160

Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu

165 170 175

Arg Leu Leu Val Met Gly Gly Gly Ile Ile Gly Leu Glu Met Gly Thr

180 185 190

Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Glu Met Phe Asp

195 200 205

Gln Val Ile Pro Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys

210 215 220

Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala

225 230 235 240

Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys

245 250 255

Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly

260 265 270

Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu

275 280 285

Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn

290 295 300

Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu

305 310 315 320

Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala

325 330 335

Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr

340 345 350

Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys

355 360 365

Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser

370 375 380

Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile

385 390 395 400

Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr

405 410 415

Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly

420 425 430

Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu

435 440 445

His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr

450 455 460

Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys

465 470

<210> 102

<211> 474

<212> PRT

<213>Artificial

<220>

<223>Lpd mutant

<400> 102

Met Ser Thr Glu Ile Lys Thr Gln Val Val Val Leu Gly Ala Gly Pro

1 5 10 15

Ala Gly Tyr Ser Ala Ala Phe Arg Cys Ala Asp Leu Gly Leu Glu Thr

20 25 30

Val Ile Val Glu Arg Tyr Asn Thr Leu Gly Gly Val Cys Leu Asn Val

35 40 45

Gly Cys Ile Pro Ser Lys Ala Leu Leu His Val Ala Lys Val Ile Glu

50 55 60

Glu Ala Lys Ala Leu Ala Glu His Gly Ile Val Phe Gly Glu Pro Lys

65 70 75 80

Thr Asp Ile Asp Lys Ile Arg Thr Trp Lys Glu Lys Val Ile Asn Gln

85 90 95

Leu Thr Gly Gly Leu Ala Gly Met Ala Lys Gly Arg Lys Val Lys Val

100 105 110

Val Asn Gly Leu Gly Lys Phe Thr Gly Ala Asn Thr Leu Glu Val Glu

115 120 125

Gly Glu Asn Gly Lys Thr Val Ile Asn Phe Asp Asn Ala Ile Ile Ala

130 135 140

Ala Gly Ser Arg Pro Ile Gln Leu Pro Phe Ile Pro His Glu Asp Pro

145 150 155 160

Arg Ile Trp Asp Ser Thr Asp Ala Leu Glu Leu Lys Glu Val Pro Glu

165 170 175

Arg Leu Leu Val Met Gly Gly Gly Ile Ile Ala Leu Glu Met Ala Thr

180 185 190

Val Tyr His Ala Leu Gly Ser Gln Ile Asp Val Val Val Arg Lys His

195 200 205

Gln Val Ile Arg Ala Ala Asp Lys Asp Ile Val Lys Val Phe Thr Lys

210 215 220

Arg Ile Ser Lys Lys Phe Asn Leu Met Leu Glu Thr Lys Val Thr Ala

225 230 235 240

Val Glu Ala Lys Glu Asp Gly Ile Tyr Val Thr Met Glu Gly Lys Lys

245 250 255

Ala Pro Ala Glu Pro Gln Arg Tyr Asp Ala Val Leu Val Ala Ile Gly

260 265 270

Arg Val Pro Asn Gly Lys Asn Leu Asp Ala Gly Lys Ala Gly Val Glu

275 280 285

Val Asp Asp Arg Gly Phe Ile Arg Val Asp Lys Gln Leu Arg Thr Asn

290 295 300

Val Pro His Ile Phe Ala Ile Gly Asp Ile Val Gly Gln Pro Met Leu

305 310 315 320

Ala His Lys Gly Val His Glu Gly His Val Ala Ala Glu Val Ile Ala

325 330 335

Gly Lys Lys His Tyr Phe Asp Pro Lys Val Ile Pro Ser Ile Ala Tyr

340 345 350

Thr Glu Pro Glu Val Ala Trp Val Gly Leu Thr Glu Lys Glu Ala Lys

355 360 365

Glu Lys Gly Ile Ser Tyr Glu Thr Ala Thr Phe Pro Trp Ala Ala Ser

370 375 380

Gly Arg Ala Ile Ala Ser Asp Cys Ala Asp Gly Met Thr Lys Leu Ile

385 390 395 400

Phe Asp Lys Glu Ser His Arg Val Ile Gly Gly Ala Ile Val Gly Thr

405 410 415

Asn Gly Gly Glu Leu Leu Gly Glu Ile Gly Leu Ala Ile Glu Met Gly

420 425 430

Cys Asp Ala Glu Asp Ile Ala Leu Thr Ile His Ala His Pro Thr Leu

435 440 445

His Glu Ser Val Gly Leu Ala Ala Glu Val Phe Glu Gly Ser Ile Thr

450 455 460

Asp Leu Pro Asn Pro Lys Ala Lys Lys Lys

465 470

<210> 103

<211> 1548

<212> DNA

<213>Escherichia coli (Escherichia coli)

<400> 103

atgacggacc atacaatgaa gaaaaacccc gtaagtatac cacacaccgt ctggtacgcc 60

gacgatatcc gccgcggaga acgcgaggcg gcagatgtgc tggggctcac actctatgag 120

ctgatgcttc gcgctggcga ggccgcattc caggtgtgtc gttcggcgta tcctgacgcc 180

cgccactggc tggtgctgtg cggtcatggt aataacggcg gcgatggcta cgtggtcgcg 240

cgactggcca aagcggtcgg cattgaggtc acgttgttgg cccaggagag cgacaaaccg 300

ttgccggaag aggccgcgct ggcacgcgaa gcatggttaa acgcgggtgg cgagatccat 360

gcttcgaata ttgtctggcc cgaatcggta gatctgattg ttgatgcgct gctcggtacc 420

ggtttgcggc aagcgccccg cgaatccatt agccagttaa tcgaccacgc taattcccat 480

cctgcgccga ttgtggcggt tgatatccct tccggcctgc tggctgaaac tggcgctacg 540

ccaggcgcgg tgatcaacgc cgatcacacc atcactttta ttgcgctgaa accaggcttg 600

ctcactggaa aagcgcggga tgttaccgga caactgcatt ttgactcact ggggctggat 660

agttggctgg caggtcagga gacgaaaatt cagcggtttt cagcagaaca actttctcac 720

tggctaaaac cgcgtcgccc gacttcgcat aaaggcgatc acgggcggct ggtaattatc 780

ggtggcgatc acggcacggc gggggctatt cgtatgacgg gggaagcggc gctgcgtgct 840

ggtgctggtt tagtccgagt actgacccgc agtgaaaaca ttgcgccgct gctgactgca 900

cgaccggaat tgatggtgca tgaactgacg atggactctc ttaccgaaag cctggaatgg 960

gccgatgtgg tggtgattgg tcccggtctg ggccagcaag agtgggggaa aaaagcactg 1020

caaaaagttg agaattttcg caaaccgatg ttgtgggatg ccgatgcatt gaacctgctg 1080

gcaatcaatc ccgataagcg tcacaatcgc gtgatcacgc cgcatcctgg cgaggccgca 1140

cggttgttag gctgttccgt cgctgaaatt gaaagtgacc gcttacattg cgccaaacgt 1200

ctggtacaac gttatggcgg cgtagcggtg ctgaaaggtg ccggaaccgt ggtcgccgcc 1260

catcctgacg ctttaggcat tattgatgcc ggaaatgcag gcatggcgag cggcggcatg 1320

ggcgatgtgc tctctggtat tattggcgca ttgcttgggc aaaaactgtc gccgtatgat 1380

gcagcctgtg caggctgtgt cgcgcacggt gcggcagctg acgtactggc ggcgcgtttt 1440

ggaacgcgcg ggatgctggc aaccgatctc ttttccacgc tacagcgtat tgttaacccg 1500

gaagtgactg ataaaaacca tgatgaatcg agtaattccg ctccctga 1548

<210> 104

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks xylAB 1

<400> 104

acgacatcat ccatcacccg cggcattacc tgattatgga gttcaatatg 50

<210> 105

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks xylAB 2

<400> 105

cccccacccg gtcaggcagg ggataacgtt tacgccatta atggcagaag 50

<210> 106

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks yjhH 1

<400> 106

aatgcgcgaa gttgccgact tcctgattaa taaaggggtc gacgggctgt 50

<210> 107

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks yfhH 2

<400> 107

gtaccgactt aactgtgttg atcatcgtac gcaagtgacc aacgctgtcg 50

<210> 108

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks yagE 1

<400> 108

ggcggcacca acgcccggga aaccatcgaa ctcagccagc acgcgcagca 50

<210> 109

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks yagE 2

<400> 109

agcacggtga agtgcggatg ggcacctttg acggtatgga tcatgctgcg 50

<210> 110

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks yiaE 1

<400> 110

catatttcag gctaaggtga tcgccttatc agtgaatgga gagaagcatg 50

<210> 111

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks yiaE 2

<400> 111

tatcgggctt tactctacgc agtcgcggct tagtccgcga cgtgcggatt 50

<210> 112

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks ycdW 1

<400> 112

aacgataagt gcgaataaat ttcgcacaac gcttttcggg agtcagtatg 50

<210> 113

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer lacks ycdW 2

<400> 113

ccaaggatag caggaatcct gatgctttat tagtagccgc gtgcgcggtc 50

<210> 114

<211> 100

<212> DNA

<213>Artificial

<220>

<223>Primer lacks ptsG 1

<400> 114

atgtttaaga atgcatttgc taacctgcaa aaggtcggta aatcgctgat gctgccggta 60

tccgtactgc ctatcgcagg tgtaggctgg agctgcttcg 100

<210> 115

<211> 100

<212> DNA

<213>Artificial

<220>

<223>Primer lacks ptsG 2

<400> 115

ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa 60

aatcgcctga acaccagaac catatgaata tcctccttag 100

<210> 116

<211> 21

<212> DNA

<213>Artificial

<220>

<223> RBS120

<400> 116

atccggtata ggaggtatag a 21

<210> 117

<211> 100

<212> DNA

<213>Artificial

<220>

<223>Primer lacks udhA 1

<400> 117

ggtgcgcgcg tcgcagttat cgagcgttat caaaatgttg gcggcggttg cacccactgg 60

ggcaccatcc cgtcgaaagc catatgaata tcctccttag 100

<210> 118

<211> 100

<212> DNA

<213>Artificial

<220>

<223>Primer lacks udhA 2

<400> 118

cccagaatct cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg 60

acgatttgtg cgcgtgccag tgtaggctgg agctgcttcg 100

<210> 119

<211> 101

<212> DNA

<213>Artificial

<220>

<223>Primer lacks pfkA 1

<400> 119

gttcctcggt tctgcgcgtt tcccggaatt ccgcgacgag aacatccgcg ccgtggctat 60

cgaaaacctg aaaaaacgtg gtgtaggctg gagctgcttc g 101

<210> 120

<211> 102

<212> DNA

<213>Artificial

<220>

<223>Primer lacks pfkA 2

<400> 120

ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc agggtaaagg 60

aatctgcctt tttccgaaat cacatatgaa tatcctcctt ag 102

<210> 121

<211> 83

<212> DNA

<213>Artificial

<220>

<223> PR01

<400> 121

acgttaaatc tatcaccgca agggataaat atctaacacc gtgcgtgttg acaattttac 60

ctctggcggt gataatggtt gca 83

<210> 122

<211> 714

<212> DNA

<213>Artificial

<220>

<223> CI857

<400> 122

atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg tcgccttaaa 60

gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt cgcagacaag 120

atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc attaaatgct 180

tataacgccg cattgcttac aaaaattctc aaagttagcg ttgaagaatt tagcccttca 240

atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc acttagaagt 300

gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc taagcttaga 360

acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct 420

gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc caagccaagc 480

tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc aggtgatttc 540

tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag ggatagcggt 600

caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa tgagagttgt 660

tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg ctga 714

<210> 123

<211> 34

<212> DNA

<213>Artificial

<220>

<223> RBS150

<400> 123

taccaactaa cgcacgttta agtaggaacc gtat 34

<210> 124

<211> 50

<212> DNA

<213>Artificial

<220>

<223>GapA is replaced by gapN

<400> 124

acgtgactga ttctaacaaa acattaacac caactggcaa aattttgtcc 50

<210> 125

<211> 50

<212> DNA

<213>Artificial

<220>

<223>GapA is replaced by gapN

<400> 125

aaaaaagagc gaccgaagtc gctcttttta gatcacagtg tcatctcaac 50

<210> 126

<211> 43

<212> DNA

<213>Artificial

<220>

<223> ptrc

<400> 126

gagctgttga caattaatca tccggctcgt ataatgtgtg gaa 43

<210> 127

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer replaces xylFGH promoters 1

<400> 127

ctaaaaattg gttacgttta tcgcggtgat tgttacttat taaaactgtc 50

<210> 128

<211> 50

<212> DNA

<213>Artificial

<220>

<223>Primer replaces xylFGH promoters 2

<400> 128

tgagtagaat gttctttatt ttcatggtgt agggccttct gtagttagag 50

Claims

1. a kind of microorganism, the microorganism is genetically modified to be used for by the way that xylose is produced into 2 into BT, 4- dihydroxy butyric acid, wherein the microorganism is used for through further genetic modification：

I) 1,2,4- butantriols are oxidized to 2,4- dihydroxy butyraldehydes；And

Ii 2,4- dihydroxy butyraldehydes) are oxidized to 2,4- dihydroxy butyric acid.

2. microorganism according to claim 1, wherein the genetic modification i) is at least one coding oxidoreducing enzyme The overexpression of gene, the oxidoreducing enzyme act on the CH-OH groups of donor.

3. microorganism according to claim 2, wherein the oxidoreducing enzyme is selected from the group：Alcohol dehydrogenase, lactic aldehyde reduction Enzyme, glyoxylate reductase, two dehydrogenation gluconic acid reductases and its any combinations.

4. microorganism according to any one of claim 1 to 3, wherein the genetic modification ii) it is at least one coding The overexpression of the gene of oxidoreducing enzyme, the oxidoreducing enzyme act on the aldehyde radical or oxo group of donor.

5. microorganism according to claim 4, wherein the oxidoreducing enzyme is selected from the group：Aldehyde dehydrogenase, aldehyde oxidase, And its any combinations.

6. microorganism according to any one of claim 1 to 5, wherein for by xylose into BT Genetic modification is the overexpression of at least one following gene：

The gene of-coding xylose dehydrogenase,

The gene of-coding xylonolactonase,

The gene of-coding xylonate dehydratase,

The gene of-coding 3- deoxidations-D- glycerine-pentulose acid decarboxylase,

The gene of-coding BT dehydrogenase, and

- its any combinations.

7. microorganism according to any one of claim 1 to 6, wherein the microorganism is used through further genetic modification In：

Iii reducing power and/or energy) are provided from the carbon source in addition to xylose, for the production of 2,4- dihydroxy butyric acid and micro- life Thing grows, and/or

Iv) at least partly suppress, preferably completely suppress carbon catabolite repression.

8. microorganism according to claim 7, wherein the genetic modification iii) be at least one following gene missing And/or reduction：

The gene of-encoding xylose isomerase,

The gene of-encoding xylulokinase,

The gene of-coding 3- deoxidations-D- glycerine-pentulosonate aldolase,

The gene of-coding ketoacid dehydrogenase, and

- its any combinations.

9. the microorganism according to claim 7 or 8, wherein the genetic modification iv) selected from following at least one：

The gene of the glucose permease of-missing coding phosphotransferase system,

The gene of-missing coding phosphate carrier Hpr albumen,

The gene and/or operator involved in the sugared input albumen system of-expression, wherein the sugar is the carbon source in addition to xylose,

- expression encodes the gene of xylose transport albumen,

- gene for encoding glucose symport albumen is overexpressed,

- gene for encoding facilitated glucose albumen is overexpressed,

- gene for encoding glucokinase is overexpressed,

The gene involved in-regulation cAMP levels, the expression of the gene of optimized encoding adenyl cyclase,

The expression of the gene of-regulation coding CRP and/or CRP sample albumen,

- expression encodes the gene of the CRP albumen independent of cAMP, and

- its any combinations.

10. microorganism according to any one of claim 1 to 9, it includes the production of the NADPH as reducing power source The further genetic modification of at least one gene involved in life, the modification are preferably selected from：

The gene of-missing or reduction encoding soluble transhydrogenase,

The gene of-missing or reduction coding phosphogvlucoisomerase,

The gene of-missing or reduction coding phosphofructokinase,

- gene for encoding glucose-6-phosphate dehydrogenase (G6PD) is overexpressed,

- mutant gene that coding can produce NADPH diaphorase is overexpressed,

- its any combinations.

11. microorganism according to any one of claim 1 to 10, wherein the bacterium belongs to enterobacteriaceae (Enterobacteriaceae), Clostridiaceae (Clostridiaceae), Bacillaceae (Bacillaceae), Streptomycetaceae Or bar Cordycepps (Corynebacteriaceae) (Streptomycetaceae).

12. microorganism according to claim 11, wherein the enterobacteriaceae lactobacteriaceae is Escherichia coli (E.coli).

13. the method for one kind production 2,4- dihydroxy butyric acid, it includes：

A) allowing, by under fermentation condition of the xylose into 2,4- dihydroxy butyric acid, to cultivate such as in the culture medium comprising xylose Genetically modified microorganism any one of claim 1 to 12, and

B) 2,4- dihydroxy butyric acid is reclaimed from the culture medium.

14. the method for claim 13, wherein the culture medium includes the carbon source in addition to xylose.

15. the method for claim 14, wherein the carbon source in addition to xylose is to include the carbon hydrate of 3,6 or 12 carbon atoms Thing, it is preferably selected from the following group：Glycerine, glucose, galactolipin, fructose, lactose, maltose, sucrose and its any combinations.