CN107674839A - A kind of method of Fusarium solani and its fermenting and producing dextranase - Google Patents
A kind of method of Fusarium solani and its fermenting and producing dextranase Download PDFInfo
- Publication number
- CN107674839A CN107674839A CN201711046501.3A CN201711046501A CN107674839A CN 107674839 A CN107674839 A CN 107674839A CN 201711046501 A CN201711046501 A CN 201711046501A CN 107674839 A CN107674839 A CN 107674839A
- Authority
- CN
- China
- Prior art keywords
- fusarium solani
- fermenting
- medium
- dextranase
- producing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000427940 Fusarium solani Species 0.000 title claims abstract description 29
- 108010001682 Dextranase Proteins 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 15
- 230000001580 bacterial effect Effects 0.000 claims abstract description 23
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 238000004321 preservation Methods 0.000 claims abstract description 9
- 244000005700 microbiome Species 0.000 claims abstract description 5
- 229920002307 Dextran Polymers 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 13
- 239000002609 medium Substances 0.000 claims description 12
- 238000012216 screening Methods 0.000 claims description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 11
- 239000001888 Peptone Substances 0.000 claims description 9
- 108010080698 Peptones Proteins 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 9
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
- 235000019319 peptone Nutrition 0.000 claims description 9
- 238000011218 seed culture Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 230000009514 concussion Effects 0.000 claims description 7
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 6
- 238000007747 plating Methods 0.000 claims description 4
- 229920001817 Agar Polymers 0.000 claims description 3
- 150000008065 acid anhydrides Chemical class 0.000 claims description 3
- 239000008272 agar Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 230000002255 enzymatic effect Effects 0.000 claims description 2
- 230000000694 effects Effects 0.000 description 5
- LWFUFLREGJMOIZ-UHFFFAOYSA-N 3,5-dinitrosalicylic acid Chemical class OC(=O)C1=CC([N+]([O-])=O)=CC([N+]([O-])=O)=C1O LWFUFLREGJMOIZ-UHFFFAOYSA-N 0.000 description 4
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 239000010282 Emodin Substances 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 108091023242 Internal transcribed spacer Proteins 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000008121 dextrose Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- RHMXXJGYXNZAPX-UHFFFAOYSA-N emodin Chemical group C1=C(O)C=C2C(=O)C3=CC(C)=CC(O)=C3C(=O)C2=C1O RHMXXJGYXNZAPX-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/77—Fusarium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2451—Glucanases acting on alpha-1,6-glucosidic bonds
- C12N9/2454—Dextranase (3.2.1.11)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01011—Dextranase (3.2.1.11)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention belongs to technical field of microbe application, more particularly to a kind of method of Fusarium solani and its fermenting and producing dextranase.A kind of Fusarium solani, characterized in that, its Classification And Nomenclature is Fusarium solani Fusarium solani DJ72, deposit number CGMCC No.14542, preservation day is on 07 27th, 2017, and depositary institution is China Committee for Culture Collection of Microorganisms's common micro-organisms center.The present invention provides a kind of new strains Fusarium solani Fusarium solani DJ72 of high yield dextranase first, and the bacterial strain can be used for fermenting and producing dextranase, and its fermentation broth enzyme vigor reaches 124.3U/ml.
Description
Technical field
The invention belongs to technical field of microbe application, more particularly to a kind of Fusarium solani and its fermenting and producing dextrose
The method of acid anhydride enzyme.
Background technology
Dextranase is that one kind being capable of α -1, the hydrolase of 6- glycosidic bonds, in medical row in catalytic cleavage dextran
All play an important roll in industry, sugar industry.In pharmaceuticals industry, dextranase can hydrolyze high molecular dextran production and replace
The Dextran 10 of dextran；In sugar industry, dextranase can reduce sugared viscosity, improve the sugared rate of recovery.
The dextranase producing strains reported at present have a lot, including fungi, bacterium etc., and wherein penicillium bacterial strain is in the majority,
It is good that fungi produces the big multistability of dextranase, and enzyme activity is high, has very high researching value.
The present invention provides a kind of Fusarium solani (Fusarium solani) for producing dextran, and Fusarium solani exists
Mainly there is rheum emodin production etc. in commercial Application, there has been no the relevant report of production dextranase.
The information for being disclosed in the background section is merely intended to understanding of the increase to the general background of the present invention, without answering
It has been the prior art well known to persons skilled in the art when being considered as recognizing or implying the information structure in any form.
The content of the invention
First purpose of the invention is to provide a kind of dextranase production bacterial strain DJ72, and it is micro- that the bacterial strain has been preserved in China
Biological inoculum preservation administration committee common micro-organisms center (abbreviation CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City
No. 3, postcode：100101), preserving number CGMCCNo.14542, preservation time is：On 07 27th, 2017, Classification And Nomenclature
For Fusarium solani Fusarium solani.
The bacterial strain is from the sewage draining exit soil of sugar refinery, through plating medium primary dcreening operation, shaking flask secondary screening, is obtained after enzyme activity detects
Arrive.
The detection of dextran enzyme activity uses 3,5- dinitrosalicylic acids (3,5-dinitrosalicylate, DNS)
Method：Under optimal pH, optimum temperature, enzyme liquid and 1% (w/v) dextran T70 reaction 10min after appropriate dilution, add
DNS terminating reactions.Enzyme amount needed for 1 μm of ol reduction sugar amount of hydrolysis generation per minute is defined as a unit enzyme activity unit.
Second object of the present invention is to provide produces the right side using Fusarium solani Fusarium solani DJ72 bacterial strains
The method of sugared acid anhydride enzyme is revolved, this method is：Ferment Fusarium solani Fusarium solani DJ72 bacterial strains, then collects fermentation production
Thing, that is, obtain dextranase.
Present invention also offers the production of Fusarium solani Fusarium solani DJ72 strain fermentations dextranase
Method, comprise the following steps：
(1) the plate screening medium culture of bacterial strain：On inoculating strain to plating medium, quiescent culture at 28-37 DEG C
2-4 days；
(2) seed culture：Bacterial strain is transferred from plate screening culture medium into liquid seed culture medium, 28-37 DEG C, 150-
200rpm concussion and cultivates 2-3 days；
(3) enzymatic production：Step 2 is cultivated into gained seed to be inoculated into fermentation medium with 1-3% volume ratio, 28-
37 DEG C, concussion and cultivate detects fermentation broth enzyme vigor after 4-7 days.
Preferably, described plate screening culture medium prescription (g/L)：Dusty yeast 10；Peptone 5；K2HPO42.5；
MgSO42.5；Dextran T500 10；Agar powder 15-20；pH 5.5-6.0.
Preferably, described liquid seed culture medium formula (g/L)：Dusty yeast 5；Peptone 5；K2HPO41；MgSO4
1；Dextran T500 10；FeSO40.01；KCl 0.5；pH 5.5-6.0.
Preferably, described fermentative medium formula (g/L)：Dusty yeast 10；Peptone 5；K2HPO42.5；MgSO4
2.5；Dextran T500 10；FeSO40.01；KCl 0.5；pH 5.5-6.0.
Compared with prior art, the present invention has the advantages that：
(1) present invention provides a kind of new strains of high yield dextranase-Fusarium solani Fusarium first
solani DJ72。
(2) new strains of the present invention-Fusarium solani Fusarium solani DJ72 can be used for fermenting and producing dextran
Enzyme, and its fermentation broth enzyme vigor reaches 124.3U/ml.
Brief description of the drawings
The hypha form that Fig. 1 is Fusarium solani Fusarium solani DJ72 observes result.
Preservation information explanation
Fusarium solani Fusarium solani DJ72, its deposit number are CGMCC No.14542, and preservation date is
On 07 27th, 2017, depositary institution was China Committee for Culture Collection of Microorganisms's common micro-organisms center, preservation address
For Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica.
Embodiment
Following examples do not limit the present invention to be better understood from the present invention.Experimental method in following embodiments,
Unless otherwise specified, it is conventional method.Experiment material used, is conventional life unless otherwise specified in following embodiments
Change what reagent shop was commercially available.Quantitative experiment in following embodiments, it is respectively provided with and repeats to test three times, results averaged.
Plate screening culture medium prescription (g/L)：Dusty yeast 10；Peptone 5；K2HPO42.5；MgSO42.5；Dextran
T500 10；Agar powder 15-20；pH 5.5-6.0.
Liquid seed culture medium formula (g/L)：Dusty yeast 5；Peptone 5；K2HPO41；MgSO41；Dextran T500
10；FeSO40.01；KCl 0.5；pH 5.5-6.0.
Fermentative medium formula (g/L)：Dusty yeast 10；Peptone 5；K2HPO42.5；MgSO42.5；Dextran T500
10；FeSO40.01；KCl 0.5；pH 5.5-6.0.
Embodiment 1：The screening of bacterial strain
(1) plating medium primary dcreening operation：It is accurate to weigh 5g sugar refineries sewage draining exit soil sample, 50ml sterilized waters are added, insert shaking table
Room temperature shakes 30-60min, concussion speed 100rpm.Supernatant is taken to carry out gradient dilution after standing 30min.By gradient dilution liquid point
Plate screening culture medium is not coated on, and 28-37 DEG C is inverted culture 2-4 days.
(2) bacterial strain for producing hydrolysis circle is selected in screening flat board from step (1), access fermentation medium carries out triangular flask
Fermentation, 28-37 DEG C, enzyme activity is detected after 180rpm concussion and cultivates 4-7.
One plant of dextranase superior strain bacterial strain DJ72 is finally given, the bacterial strain has been preserved in Chinese microorganism strain guarantor
Hiding administration committee's common micro-organisms center, (abbreviation CGMCC, address are：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, it is postal
Coding：100101), preserving number is CGMCC No.14542, and the preservation time is：On 07 27th, 2017).
Embodiment 2：The identification of bacterial strain
(1) morphological analysis of bacterial strain
As shown in figure 1, the bacterium colony of the bacterial strain is neat, whitening color or faint yellow, in villiform, mycelia has every branch.Small point
Raw spore is in fusiform or oval, and macroconidium is in fusiformis or crescent.
(2) Molecular Identification of bacterial strain；Specific authentication step is as follows：Extraction bacterial strain DJ72 STb gene is simultaneously used as template, applies
Universal primer ITS1：5 '-TCCGTAGGTGAACCTGCGG-3 ' and ITS4：5 '-TCCTCCGC TTATTGATATG-3 ', PCR expansions
Increasing obtains its ITS, and 521bp nucleotide sequence is obtained through sequencing, and its sequence is shown in SEQ ID NO.1.
Through sequence analysis analysis shows：Its homology highest with Fusarium solani (Fusarium solani) bacterial strain,
Up to 100%.
According to morphology and Molecular Identification result, DJ72 is accredited as Fusarium solani (Fusarium solani).
Embodiment 3：The method of bacterial strain DJ72 fermenting and producing dextranases
(1) seed culture：Bacterial strain is transferred from plate screening culture medium into liquid seed culture medium, 28 DEG C, 150-
200rpm concussion and cultivates 2-3 days；
(2) step 1 is cultivated into gained seed and the 50L fermentations equipped with 35L fermentation mediums is inoculated into 1-3% volume ratio
In tank, 28 DEG C, cultivate 6-7 days, it is 200-250rpm to control speed of agitator, and zymotic fluid pH controls are 5.5.
Interval 12-24h streams plus 1L concentration are 10g/L dextrans T500 as feed supplement in fermentation process.
After fermentation ends, the detection final vigor of dextranase is 124.3U/ml.
The description of the foregoing specific illustrative embodiment to the present invention is to illustrate and the purpose of illustration.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to above-mentioned teaching, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explain that the certain principles of the present invention and its reality should
With so that those skilled in the art can realize and utilize the present invention a variety of exemplaries and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Sequence table
<110>Guangxi Dingle Biotechnology Co., Ltd.
<120>A kind of method of Fusarium solani and its fermenting and producing dextranase
<130> HZLT
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 521
<212> DNA
<213>Artificial sequence (a kind of method of Fusarium solani and its fermenting and producing dextranase)
<400> 1
cactcatcac cctgtgacat acctaaaacg ttgcttcggc gggaacagac ggccccgtaa 60
cacgggccgc ccccgccaga ggacccccta actctgtttc tattatgttt cttctgagta 120
aaacaagcaa ataaattaaa actttcaaca acggatctct tggctctggc atcgatgaag 180
aacgcagcga aatgcgataa gtaatgtgaa ttgcagaatt cagtgaatca tcgaatcttt 240
gaacgcacat tgcgcccgcc agtattctgg cgggcatgcc tgttcgagcg tcattacaac 300
cctcaggccc ccgggcctgg cgttggggat cggcgaggcg ccccctgcgg gcacacgccg 360
tcccccaaat acagtggcgg tcccgccgca gcttccattg cgtagtagct aacacctcgc 420
aactggagag cggcgcggcc acgccgtaaa acacccaact tctgaatgtt gacctcgaat 480
caggtaggaa tacccgctga acttaagcat atcaaataag c 521
Claims (5)
1. a kind of Fusarium solani, it is characterised in that its Classification And Nomenclature is Fusarium solani Fusarium solani DJ72, is protected
Numbering CGMCC No.14542 are hidden, preservation day is on 07 27th, 2017, and depositary institution is Chinese microorganism strain preservation management
Committee's common micro-organisms center.
2. the method for Fusarium solani fermenting and producing dextranase according to claim 1, it is characterised in that including such as
Lower step：
(1) the plate screening medium culture of bacterial strain：On inoculating strain to plating medium, quiescent culture 2-4 at 28-37 DEG C
My god；
(2) seed culture：Bacterial strain is transferred from plate screening culture medium into liquid seed culture medium, 28-37 DEG C, 150-
200rpm concussion and cultivates 2-3 days；
(3) enzymatic production：Step 2 is cultivated into gained seed to be inoculated into fermentation medium with 1-3% volume ratio, 28-37 DEG C,
Concussion and cultivate detects fermentation broth enzyme vigor after 4-7 days.
3. the method for Fusarium solani fermenting and producing dextranase according to claim 2, it is characterised in that step
(1) the plate screening culture medium prescription (g/L) described in：Dusty yeast 10；Peptone 5；K2HPO42.5；MgSO42.5；Dextrorotation
Sugared acid anhydride T500 10；Agar powder 15-20；pH 5.5-6.0.
4. the method for Fusarium solani fermenting and producing dextranase according to claim 2, it is characterised in that step
(2) the liquid seed culture medium formula (g/L) described in：Dusty yeast 5；Peptone 5；K2HPO41；MgSO41；Dextran
T500 10；FeSO40.01；KCl 0.5；pH 5.5-6.0.
5. the method for Fusarium solani fermenting and producing dextranase according to claim 2, it is characterised in that step
(3) fermentative medium formula (g/L) described in：Dusty yeast 10；Peptone 5；K2HPO42.5；MgSO42.5；Dextran
T500 10；FeSO40.01；KCl 0.5；pH 5.5-6.0.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711046501.3A CN107674839B (en) | 2017-10-31 | 2017-10-31 | Fusarium solani and method for producing dextranase by fermenting fusarium solani |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711046501.3A CN107674839B (en) | 2017-10-31 | 2017-10-31 | Fusarium solani and method for producing dextranase by fermenting fusarium solani |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107674839A true CN107674839A (en) | 2018-02-09 |
| CN107674839B CN107674839B (en) | 2020-08-25 |
Family
ID=61143061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711046501.3A Active CN107674839B (en) | 2017-10-31 | 2017-10-31 | Fusarium solani and method for producing dextranase by fermenting fusarium solani |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107674839B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486796A (en) * | 2018-12-06 | 2019-03-19 | 中诺生物科技发展江苏有限公司 | A method of preparing dextranase enzyme preparation |
| CN114231422A (en) * | 2021-12-08 | 2022-03-25 | 农业农村部环境保护科研监测所 | Fusarium solani for degrading fomesafen and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0387178A (en) * | 1989-08-30 | 1991-04-11 | Nippon Shokuhin Kako Co Ltd | Novel endodextrase, its production and production of isomaltooligosaccharide using the same enzyme |
| KR20170045914A (en) * | 2015-10-20 | 2017-04-28 | 서울대학교산학협력단 | Oral health care materials for pets |
-
2017
- 2017-10-31 CN CN201711046501.3A patent/CN107674839B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0387178A (en) * | 1989-08-30 | 1991-04-11 | Nippon Shokuhin Kako Co Ltd | Novel endodextrase, its production and production of isomaltooligosaccharide using the same enzyme |
| KR20170045914A (en) * | 2015-10-20 | 2017-04-28 | 서울대학교산학협력단 | Oral health care materials for pets |
Non-Patent Citations (2)
| Title |
|---|
| EISHUN SHIMIZU等: "Purification and Characterization of an isomaltotriose-producing endo-dextranase from a Fusarium sp.", 《BIOSCI.BIOTECHNOL.BIOCHEM.》 * |
| LLOYD G. SIMONSON等: "Characterization of an Extracellular Dextranase from Fusarium moniliforme", 《APPLIED MICROBIOLOGY》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109486796A (en) * | 2018-12-06 | 2019-03-19 | 中诺生物科技发展江苏有限公司 | A method of preparing dextranase enzyme preparation |
| CN114231422A (en) * | 2021-12-08 | 2022-03-25 | 农业农村部环境保护科研监测所 | Fusarium solani for degrading fomesafen and application thereof |
| CN114231422B (en) * | 2021-12-08 | 2023-12-01 | 农业农村部环境保护科研监测所 | Fusarium solani for degrading fomesafen and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107674839B (en) | 2020-08-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100334200C (en) | Glutamic acid capable of having high-yield glutamine | |
| CN102191203B (en) | Bacillus amyloliquefaciens and method for producing chymosin by fermenting using same | |
| Hesham et al. | Phylogenetic analysis of isolated biofuel yeasts based on 5.8 S-ITS rDNA and D1/D2 26S rDNA sequences | |
| CN102586151B (en) | High-yield polysaccharide strain and method for producing polysaccharide by utilizing strain through fermentation | |
| CN103740605B (en) | Bacillus aquimaris and application thereof | |
| CN104498365A (en) | Bacterial strain capable of producing chitin deacetylase and application of bacterial strain in production of chitin deacetylase through fermentation | |
| CN104263650A (en) | A high-tannase-yield strain and a preparing method thereof | |
| CN104893997B (en) | A kind of bacterial strain and its fermentation process of temperature production chitinase | |
| CN104894024B (en) | One plant of Pseudoalteromonas mutant strain and its application | |
| CN101195801A (en) | Glossy ganoderma polyoses producing equipment and technique | |
| CN102399726B (en) | Sporosarcina and application thereof | |
| CN103937691B (en) | One plant production β fructosidases aspergillus oryzae strain and its cultural method and application | |
| CN105802892B (en) | It is a kind of produce keratinase germ oligotrophy unit cell and its application | |
| CN104046586B (en) | One strain gene engineering bacterium and the application in producing (2R, 3R)-2,3-butanediol thereof | |
| CN106434475B (en) | One streptomycete category polysaccharide degradation bacteria and its cultural method and application | |
| CN107674839A (en) | A kind of method of Fusarium solani and its fermenting and producing dextranase | |
| CN104762229B (en) | A kind of bacillus subtilis strain and its application | |
| CN105602917A (en) | Production method of hesperidinase and application | |
| CN106754486A (en) | One plant height produces pseudomonad and its enzymatic production method of trehalose synthase | |
| CN101544958B (en) | High-yield paenibacillus amyloliquefaciens csuft F14 for chitin deacetylase and its application | |
| CN105950482B (en) | One plant of bacterial strain for producing inulinase and its application | |
| CN101993846B (en) | Bacillus subtilis and method for producing chymosin by using same | |
| CN105112320B (en) | A kind of Burkholderia gladioli strain and its method for fermentation production of alkaline lipase | |
| JP5083735B2 (en) | Clostridial genus, cellulase-containing cellulase and method for producing hemicellulase | |
| CN106754829A (en) | A kind of method of utilization bacillus HS17 fermenting and producing chitosan enzymes and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20240616 Address after: No. 805-1, 8th Floor, Building E4, Nanning ASEAN Enterprise Headquarters Port, No.10 Xinji Road, Nanning City, Guangxi Zhuang Autonomous Region, 530000 Patentee after: GUANGXI DUODELE BIOTECHNOLOGY Co.,Ltd. Country or region after: China Address before: 13th Floor, Grain Building, No. 86 Zhenghe Road North, Xingbin District, Laibin City, Guangxi Zhuang Autonomous Region, 546100 Patentee before: GUANGXI DINGLE BIOTECHNOLOGY CO.,LTD. Country or region before: China |