CN107661454A - A kind of microwave radiation exaraction brown rice polyphenol technique - Google Patents
A kind of microwave radiation exaraction brown rice polyphenol technique Download PDFInfo
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- CN107661454A CN107661454A CN201711075141.XA CN201711075141A CN107661454A CN 107661454 A CN107661454 A CN 107661454A CN 201711075141 A CN201711075141 A CN 201711075141A CN 107661454 A CN107661454 A CN 107661454A
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- brown rice
- polyphenol
- microwave radiation
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- radiation exaraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a kind of microwave radiation exaraction brown rice polyphenol technique, belong to brown rice deep process technology field.Including：1) the pretreated brown rice milling of microwave pulse, crosses 40 mesh sieves；2) it is 1 to add feed liquid mass ratio:30‑1:50 ethanol mixing, concentration of alcohol are 70 90%；3) mixed serum is placed in 40 60 DEG C of constant temperature, through the 90min of ultrasonic-leaching 80；4) centrifuge；5) by the filtrate rotary evaporated to dryness after centrifugation, add methanol and dissolve to obtain polyphenolic extract.The microwave radiation exaraction brown rice polyphenol technique of the present invention, its operating procedure simple and fast, preparation process efficient energy-saving and preparation cost are low, this process makes full use high penetrating power of microwave and the high efficiency of heating surface, drastically increase the yield of brown rice polyphenol, the comprehensive utilization ratio and its surcharge of brown rice are improved, there are wide market prospects.
Description
Technical field
The invention belongs to brown rice deep process technology field, and in particular to a kind of processing work of microwave radiation exaraction brown rice polyphenol
Skill.
Background technology
Brown rice is not only containing abundant protein, fat, cellulose, vitamin B1, B2 and mineral matter, also rich in rice bran fat
The various active materials such as polysaccharide, oryzanol, γ-Hi-Z, γ-aminobutyric acid, phenols.Polyphenols is main in brown rice
Nutrient for plants, while be also natural anti-reflecting oxide.Epidemiological study shows that the antioxidation activity of cereal can be effectively pre-
Anti- chronic disease and metabolic disease occur.
The domestic research to brown rice is concentrated mainly on the extraction work of the materials such as polysaccharide, protein, starch and cellulose at present
In skill and functional characteristic, and it is less to being related in terms of the polyphenols such as flavones in brown rice, for polyphenols in brown rice
Extraction typically use organic solvent extraction, in order to improve yield can take extend extraction time, improve Extracting temperature or
The methods of extracting again is soaked to raw material to improve yield, also there was only a small amount of document at home for brown rice Polyphenols
Extraction has carried out Primary Study.Therefore the processing technology for improving the extraction efficiency of polyphenol in brown rice is to be worth studied.
The content of the invention
Goal of the invention：For above-mentioned the shortcomings of the prior art, there is provided a kind of microwave radiation exaraction brown rice polyphenol work
Skill, there is operating procedure simple and fast, preparation process efficient energy-saving and prepare low cost and other advantages.
Technical scheme：In order to realize foregoing invention purpose, the technical solution adopted by the present invention is：
A kind of microwave radiation exaraction brown rice polyphenol technique, comprises the following steps：
(1) brown rice is placed in microwave equipment and enters horizontal pulse processing, microwave radiation dosage is 3.0-5.0kWkg-1, processing
Time 60-120s, pulse working time 10-20s, interval time 15s-25s, it is standby that plastic packaging bag is put into after brown rice is cooled to room temperature
With；
(2) brown rice obtained by step (1) is crushed to 30-50 mesh sieves, it is standby；
(3) solid-liquid ratio 1 is pressed:30-1:The ethanol that 50 addition volume fractions are 70-90%, 40-60 DEG C is placed in after well mixed
Constant temperature, through ultrasonic-leaching 80-90min；
(4) material in step (3) is centrifuged, it is brown rice polyphenol extract solution to take whole supernatants；
(5) brown rice extract solution is evaporated to dryness with Rotary Evaporators, brown rice polyphenolic extract is obtained after being dissolved with methanol.
In step (1), microwave radiation dosage is 3.0-4.0kWkg-1, processing time 60-90s.
In step (3), microwave radiation dosage is 4.0kWkg-1, processing time 90s, pulse working time 10s, during interval
Between 20s.
In step (3), ultrasonic-leaching power is 200W, frequency 40kHz.
In step (4), the rotating speed of centrifugation is 12000r/min, time 5min.
In step (5), rotary evaporation bath temperature is 50 DEG C.
Used in the present invention is that frequency is 2450MHz microwave devices, microwave and other electromagnetic waves for radiant heating
Compare, such as far infrared, equiwavelength is longer, therefore has more preferable penetrability.When microwave penetrates medium, due to microwave energy and medium
Certain interaction occurs, the molecule of medium is produced high-frequency vibration, the intermolecular of medium produces mutually friction, caused Jie
The rise of matter temperature, make dielectric material it is internal, it is outside almost simultaneously heat temperature raising, formed body heat source state, substantially reduce often
Heat conduction time in rule heating.
Beneficial effect：Compared with prior art, microwave radiation exaraction brown rice polyphenol technique of the invention, the letter of its operating procedure
Single quick, preparation process efficient energy-saving and prepare that cost is low, this process makes full use high penetrating power of microwave and high heating are imitated
Rate, the yield of brown rice polyphenol is drastically increased, improve the comprehensive utilization ratio of brown rice, there are wide market prospects.The party
The polyphenol that method is extracted from brown rice is natural antioxidant, has lowering blood pressure and blood fat and other effects.The present invention improves brown rice
The extraction efficiency of polyphenol, comprehensive utilization can be carried out to brown rice and improves added value of product.
Brief description of the drawings
Fig. 1 is the canonical plotting of polyphenol concentration (in terms of gallic acid)；
Fig. 2 is embodiment and the polyphenol content result figure measured by reference examples.
Embodiment
With reference to specific embodiment, the present invention is described further.
Embodiment 1
A kind of microwave radiation exaraction brown rice polyphenol technique, step are as follows：
(1) microwave pulse processing brown rice：Brown rice is placed in microwave equipment and enters horizontal pulse processing, microwave radiation dosage is
3.0kW·kg-1, processing time 60s, pulse working time 10s, interval time 20s, plastic packaging is put into after brown rice is cooled to room temperature
Bag is standby；
(2) brown rice crushes：Brown rice obtained by step (1) is crushed to 40 mesh sieves, it is standby；
(3) ultrasonic extraction：By solid-liquid ratio 1:The ethanol that 40 addition volume fractions are 80%, 50 DEG C of perseverances are placed in after well mixed
Temperature, through ultrasonic-leaching 80min；
(4) centrifuge：Material 12000r/min in step (3) is centrifuged into 5min, it is that brown rice is more to take whole supernatants
Phenol extraction liquid；
(5) by above-mentioned brown rice extract solution with being evaporated to dryness at 50 DEG C of Rotary Evaporators, it is more to obtain brown rice after being dissolved with methanol
Phenol crude extract；Determine polyphenol content.
Embodiment 2
Microwave radiation dosage is 3.0kWkg when microwave pulse handles brown rice-1, processing time 90s, its extracting method and reality
It is identical to apply example 1.
Embodiment 3
Microwave radiation dosage is 3.0kWkg when microwave pulse handles brown rice-1, processing time 120s, its extracting method with
Embodiment 1 is identical.
Embodiment 4
Microwave radiation dosage is 4.0kWkg when microwave pulse handles brown rice-1, processing time 60s, its extracting method and reality
It is identical to apply example 1.
Embodiment 5
Microwave radiation dosage is 4.0kWkg when microwave pulse handles brown rice-1, processing time 90s, its extracting method and reality
It is identical to apply example 1.
Embodiment 6
Microwave radiation dosage is 4.0kWkg when microwave pulse handles brown rice-1, processing time 120s, its extracting method with
Embodiment 1 is identical.
Embodiment 7
Microwave radiation dosage is 5.0kWkg when microwave pulse handles brown rice-1, processing time 60s, its extracting method and reality
It is identical to apply example 1.
Embodiment 8
Microwave radiation dosage is 5.0kWkg when microwave pulse handles brown rice-1, processing time 90s, its extracting method and reality
It is identical to apply example 1.
Embodiment 9
Microwave radiation dosage is 5.0kWkg when microwave pulse handles brown rice-1, processing time 120s, its extracting method with
Embodiment 1 is identical.
Reference examples：
(1) brown rice crushes：Original brown rice without any processing is crushed to 40 mesh sieves, it is standby；
(2) ultrasonic extraction：By solid-liquid ratio 1:The ethanol that 40 addition volume fractions are 80%, 50 DEG C of perseverances are placed in after well mixed
Temperature, through ultrasonic-leaching 80min；
(3) centrifuge：Material 12000r/min in step (2) is centrifuged into 5min, it is that brown rice is more to take whole supernatants
Phenol extraction liquid；
(4) by above-mentioned brown rice extract solution with being evaporated to dryness at 50 DEG C of Rotary Evaporators, it is more to obtain brown rice after being dissolved with methanol
Phenol crude extract.Determine polyphenol content.
Above-described embodiment and polyphenol content assay method obtained by reference examples are as follows：
Using the yield of Folin-Ciocalteu colorimetric method for determining brown rice polyphenol：
(1) making of standard curve：Accurate μ g/mL of gallic acid standard liquid 10 for preparing various concentrations, 20 μ g/mL,
30μg/mL、40μg/mL、50μg/mL.The accurate gallic acid standard liquid 0.125mL that draws adds deionized water 0.5mL and good fortune
Woods phenol 0.125mL, mixing stand the Na of addition 7% after 6min2CO3Solution 1.25mL and 1.0mL deionized water, it is placed in 25 DEG C of perseverances
Temperature reaction 90min determines its absorbance after 760nm wavelength, and each concentration mensuration is three times.Draw gallic acid standard curve.
Regression equation and phase of the absorbance to concentration of standard solution it can be seen from the standard curve of Fig. 1 gallic acids
Relation number is respectively y=0.0031x+0.0481, R2=0.9997 show in the concentration range it is linear good.
(2) in sample polyphenol content measure：Polyphenolic extract in accurate extraction embodiment and reference examples add go from
Sub- water 0.5mL and forint phenol 0.125mL, mixing stand the Na of addition 7% after 6min2CO3Solution 1.25mL and 1.0mL deionization
Water, it is placed in 25 DEG C of isothermal reaction 90min and determines its absorbance after 760nm wavelength, each sample measure is three times.Utilize what is obtained
Standard curve calculates corresponding polyphenol concentration (in terms of gallic acid).Polyphenol content is that gallic acid contains in 100g coarse rice powders
Measure (mg GAE/100g).
Fig. 2 is that the brown rice polyphenol content of microwave radiation exaraction extracts the contrast of polyphenol content with untreated original brown rice.
As shown in Figure 2, the present invention by suitable microwave condition assisted extraction brown rice polyphenol than traditional method for extracting brown rice polyphenol efficiency
It is higher.When microwave radiation dosage is 4.0kWkg-1, when the microwave operational time is 90s, brown rice polyphenol extraction efficiency highest, polyphenol
Content is 76.72mg GAE/100g.
Claims (6)
1. a kind of microwave radiation exaraction brown rice polyphenol technique, it is characterised in that comprise the following steps：
(1) brown rice is placed in microwave equipment and enters horizontal pulse processing, microwave radiation dosage is 3.0-5.0kWkg-1, processing time
60-120s, pulse working time 10-20s, interval time 15s-25s, it is standby that plastic packaging bag is put into after brown rice is cooled to room temperature；
(2) brown rice obtained by step (1) is crushed to 30-50 mesh sieves, it is standby；
(3) solid-liquid ratio 1 is pressed:30-1:The ethanol that 50 addition volume fractions are 70-90%, 40-60 DEG C of perseverance is placed in after well mixed
Temperature, through ultrasonic-leaching 80-90min；
(4) material in step (3) is centrifuged, it is brown rice polyphenol extract solution to take whole supernatants；
(5) brown rice extract solution is evaporated to dryness with Rotary Evaporators, brown rice polyphenolic extract is obtained after being dissolved with methanol.
2. microwave radiation exaraction brown rice polyphenol technique according to claim 1, it is characterised in that in step (1), microwave spoke
It is 3.0-4.0kWkg to penetrate dosage-1, processing time 60-90s.
3. microwave radiation exaraction brown rice polyphenol technique according to claim 1, it is characterised in that in step (3), microwave spoke
It is 4.0kWkg to penetrate dosage-1, processing time 90s, pulse working time 10s, interval time 20s.
4. microwave radiation exaraction brown rice polyphenol technique according to claim 1, it is characterised in that in step (3), ultrasonic wave
Extraction power is 200W, frequency 40kHz.
5. microwave radiation exaraction brown rice polyphenol technique according to claim 1, it is characterised in that in step (4), centrifugation point
From rotating speed be 12000r/min, time 5min.
6. microwave radiation exaraction brown rice polyphenol technique according to claim 1, it is characterised in that in step (5), rotation is steamed
The bath temperature that floods is 50 DEG C.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886577A (en) * | 2015-06-16 | 2015-09-09 | 福建省农业科学院农业工程技术研究所 | Preparation method of prunus fruit polyphenol |
| CN105250356A (en) * | 2015-09-10 | 2016-01-20 | 黑龙江八一农垦大学 | Method for mung bean polyphenol with solvent extraction in synergistic assistance of microwaves and supersonic waves |
| CN106619744A (en) * | 2017-02-08 | 2017-05-10 | 中英阿诺康（宁夏）生物科技有限公司 | Extraction technique and preparation method of algal polyphenols |
-
2017
- 2017-11-06 CN CN201711075141.XA patent/CN107661454A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886577A (en) * | 2015-06-16 | 2015-09-09 | 福建省农业科学院农业工程技术研究所 | Preparation method of prunus fruit polyphenol |
| CN105250356A (en) * | 2015-09-10 | 2016-01-20 | 黑龙江八一农垦大学 | Method for mung bean polyphenol with solvent extraction in synergistic assistance of microwaves and supersonic waves |
| CN106619744A (en) * | 2017-02-08 | 2017-05-10 | 中英阿诺康（宁夏）生物科技有限公司 | Extraction technique and preparation method of algal polyphenols |
Non-Patent Citations (1)
| Title |
|---|
| 周义等: "小米多酚提取方法的研究进展", 《农产品加工》 * |
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Application publication date: 20180206 |