CN107647113A - Promote the preparation method of cultured freshwater fish growth feed - Google Patents
Promote the preparation method of cultured freshwater fish growth feed Download PDFInfo
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- CN107647113A CN107647113A CN201710950838.0A CN201710950838A CN107647113A CN 107647113 A CN107647113 A CN 107647113A CN 201710950838 A CN201710950838 A CN 201710950838A CN 107647113 A CN107647113 A CN 107647113A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a kind of preparation method for promoting cultured freshwater fish growth feed, including：The preparation of liposome, the preparation of Chinese medicine preparation, bran modification, fermented bean dregs, compounding.Have the beneficial effect that：By modified and fermentation, the ANFs such as urease activity, trypsin ihhibitor, stachyose, raffinose in feed are significantly reduced, aflatoxins content is also effectively controlled, so as to reduce indigestion and the allergic reaction after fish edible bean cake, efficiency of feed utilization is improved, improves breeding efficiency；Feed nutrition general equilibrium, not only grown the nutrients such as necessary protein, amino acid, aliphatic acid, vitamin, mineral matter rich in fresh-water fishes, and feed palatability is good, improve food-intake, fish internal system, regulation metabolism can also be stimulated, improve efficiency of feed utilization, be a kind of comprehensive, efficient, safety feed for promoting cultured freshwater fish growth.
Description
Technical field
The present invention relates to the preparation field of fresh water fish feed, promotes cultured freshwater fish to grow feed more particularly, to a kind of
Preparation method.
Technical background
In a broad sense, fresh-water fishes refer to the fish that can be lived in the fresh water that salinity is 3/1000ths.Known fish in the world
Kind more than 26000 is there are about, is a major class of most species in vertebrate, accounts for the 48.1% of vertebrate sum.They are most absolutely
Number is lived in ocean, and fresh-water fishes there are about more than 8600 and plant.Nearly 3000 kinds of the existing fish in China, wherein fresh-water fishes have more than 1000 to plant.
Most fresh-water fishes have special a color and speckle, or body colour is consistent with surrounding environment, can it is hidden oneself, or confuse the enemy and hunt
Thing, to protect oneself or stalk prey.In shallow water, the body colour of the fish generally back of the body is blue or green, green, and abdomen is shallow white, these color quilts
Referred to as eliminate color, the bottom is hoped, and be thought as sky, prestige is gone down, then feel be extra large water ﹔ and in deep water, the very cloudy Shen of body colour, Chang Wei
The color such as dark red, black.As long as substantially there is the place of fresh water, just there is fresh-water fishes inhabitation, up to warm pleasant hot spring, under to freeze into
The south poles of cardiopulmonary, it can all find the trace of fresh-water fishes.Rivers and creeks upstream about more than 1500 meters of height above sea level, current are anxious, there is a large amount of great Shi
Pond that head is formed, the habitat such as stone gap, such as：Silver xenocypris Yu, Xia tiger.About height above sea level 200-1500 rice, landform can be rather in rivers and creeks middle reaches
Complexity, there are a variety of habitats such as flat rapids, anxious rapids, Pingtan, pool, waterfall, Jian Dao, backwater, such as：Stone carp, sweetfish.Rivers and creeks downstream
The flow of water is gentle, vulnerable to pollution, and local fish is often substituted by the high external fingerling of resistance to soiling, such as：Oldwife, Chinese lute mouse fish.Fresh water
Fish mixed breeding be reservoir, pond launch fingerling during increase year by year xenogenesis, different age fish mixed breeding ratio one kind raising side
Formula.In terms of current fresh fish market, fresh-water fishes mixed breeding can bring good economic benefit and social benefit.
Preparation on fresh water fish feed has many methods, and prior art is as authorized public number
The B of CN 103340334 Chinese invention patent, discloses a kind of fresh water fish feed and preparation method thereof, the inventive method
By imported fish meal, peanut meal, rape cake, flour, farina, brewer's yeast, dregs of beans, corn flour, cooked Chicken Albumin
Deng preparing fresh water fish feed after crushing compound, the nutritional need of fresh-water fishes is disclosure satisfy that, improves the immunity of fresh-water fishes, promotes intestines
Road absorbs, and improves the production performance of fresh-water fishes, but the imported fish meal in the inventive method is expensive, and dregs of beans, peanut
The dregs of rice are not fermented to be just added into feed, urase, trypsin ihhibitor, stachyose, raffinose in dregs of beans, peanut meal etc.
ANFs can reduce the nutritional ingredient of raw material, and the aflatoxins in dregs of beans can pollute, or even can cause fish
Indigestion and allergic reaction, therefore its promotional value is little.
The content of the invention
The purpose of this method is to provide a kind of preparation method for promoting cultured freshwater fish growth feed, this preparation method
Raw material are both from the discarded object in industrial and agricultural production, and realizing turns waste into wealth, and the feed palatability of preparation is good, nutritious
Comprehensively.
Used microorganism and its bought enterprise are in the inventive method：Lactobacillus paracasei, Lactobacillus salivarius, Shan
Xi Senfu natural products Co., Ltd；Bacillus licheniformis, Cangzhou Lai Sen Bioisystech Co., Ltd.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Cultured freshwater fish growth is promoted to raise
The preparation method of material, including：The preparation of liposome, the preparation of Chinese medicine preparation, bran modification, fermented bean dregs, compounding, are specifically included
Following steps：
The preparation of liposome：3-4 part Tea Polyphenols powder is dissolved among 60-75 part absolute ethyl alcohols, ultrasonic dissolution, adds 8-10 parts
Egg yolk lecithin and the dissolving of 2.0-2.5 part cholesterol mixed solutions stirring in water bath are fast by above-mentioned lipid ethanol solution with syringe
Speed is injected into the Na that 80-100 parts pH is 7.2-7.52HPO4-KH2PO4In buffer solution, keep pH constant, at a temperature of 35-38 DEG C
Stirring hydration 15-25 minutes, tea polyphenol nano lipidosome suspension is obtained, suspension is flung into residual second through depressurizing rotary evaporation
Alcohol, produce tea polyphenol nano lipidosome；Tea Polyphenols has inoxidizability, detoxication, radiation resistance, and with removing certainly
By the effect of base；The phospholipid liposome that this method makes is easy to the absorption of fish, and preparation method is simple, is kept away in preparation process
Exempt from the physics and chemistry processing containing potentially harmful substances such as ether, be especially suitable for industrialization large-scale production；
The preparation of Chinese medicine preparation：By 3-5 parts Radix Codonopsis, 5-6 parts honeysuckle, 6-7 parts Divine Comedy, 6-7 parts bighead atractylodes rhizome, 3-5 parts Radix Astragali, 10-
15 parts of masts are crushed with pulverizer, cross 80-90 mesh sieves, compound Chinese medicinal preparation is made；Compound Chinese medicinal preparation is weighed, with degreasing cotton yarn
Wrap, add 8-10 times of distilled water, it is boiled after immersion 1-2 hours, 30-45 minutes are cooked by slow fire, are filtered with gauze, are filtered
Slag is decocted again, and filtrate is mixed, and low temperature is concentrated into aqueous 35-40%, is saved backup at 3-5 DEG C；Chinese medicine preparation can strengthen
The disease-resistant antibacterial ability of fish, immunity of organisms is improved, is built up health, and the taste of Chinese medicine preparation uniqueness is advantageous to enhancing and lured
Eat the food calling ability of agent；
Bran modification：Miscible in the water of 20-25 times of weight with wheat bran powder, regulation pH is 5.0-6.0, adds wheatfeed 2-
4% cellulase, bifrequency ultrasound-assisted enzymolysis digest 1-3 hours under the conditions of 50-55 DEG C, and heating enzyme deactivation terminates anti-
Should, enzymolysis modified dietary fiber is made；Enzymolysis modified bran dietary fiber surface irregularity, the water holding of modified dietary fiber
Power, expansive force, dissolubility and viscosity and adsorption capacity to fat, cholesterol, nitrite ion all accordingly improve, modified
The dietary fiber molecular weight and molecular weight of wheat bran afterwards, the edibility of wheat bran is improved, suitably as aquatic animal feed；
Fermented bean dregs：By dregs of beans and water according to solid-liquid ratio 1:2-3（g/ml）Uniformly mixing, in microwave power 800-1000W conditions
Lower microwave treatment 2-3 minutes, 4-6% Lactobacillus paracasei, Lactobacillus salivarius, bacillus licheniformis hybrid bacterial strain are inoculated with,
At a temperature of 39-40 DEG C, ferment 48-72 hours, bean cake powder is made in drying and crushing after the completion of fermentation；Bean pulp fermentation can be reduced effectively
The ANFs such as urease activity, trypsin ihhibitor, stachyose, raffinose in powder, aflatoxins pollution is reduced,
So as to reduce indigestion and the allergic reaction after fish edible bean cake, efficiency of feed utilization is improved, reduces the probability of fish diseases,
Improve breeding efficiency；
Compounding：Weigh organic fertilizer 70-80 parts, modified bean pulp powder 70-80 parts, modified wheat bran 30-35 parts, liposome 12-15 parts,
Fish guts oil 30-35 parts, fish protein hydrolyzate 35-40 parts, dextrin 8-11 parts, alphalise starch 20-25 parts, yeast cell wall 5-6 parts,
Sodium alginate 3-4 parts, fennel oil 0.8-1.0 parts, functional polypeptide 1.0-1.2 parts, multidimensional 3.2-3.5 parts, more ore deposit 4.2-4.5
Part, enzyme preparation 1.3-1.4 parts, growth accelerator 7-8 parts, preservative 0.5-0.8 parts are well mixed, and cross 60-80 mesh sieves after drying, make
Grain produces the feed for promoting cultured freshwater fish growth, particle diameter 0.2-0.6mm；The amino acid sequence of functional polypeptide is：
SCASRCCRARCGKSRCRARRCRARRCGCRARRCGYYVSVFRC；Functional polypeptide can stimulate the liver and intestines of fish
Road, promote the secretion of fatty,fiss enzyme and amylase, improve the activity of enzyme, promote feed of the fish to feed with digesting and assimilating,
Promote growth, put on weight, shorten the culture-cycle, reduce aquaculture cost；The main raw material(s) of feed is given birth to both from industrial or agricultural
The waste products of production, carried out being used as feed after processing removes anti-nutrient substance, not only improve the economic value of material,
Realize full effect to utilize, and protect environment, reduce the cost of processing fertilizer；Promote the feed battalion of cultured freshwater fish growth
General equilibrium is supported, is not only grown necessary protein, amino acid, aliphatic acid, vitamin, mineral matter etc. rich in fresh-water fishes
Nutrient, and feed can stimulate the smell of fish, the sense of taste, vision, attract fish collection to promote fish around feed
Feed, food-intake is improved, fish internal system, regulation metabolism can also be stimulated, improve efficiency of feed utilization, improve fish
Health, the production performance of fish is improved, be a kind of feed for promoting cultured freshwater fish growth comprehensively, efficiently, safe.
Preferably, the frequency of bifrequency ultrasonic wave is 25-28kHz and 35-45kHz, ultrasound intensity 0.35-0.45w/
cm2；Bifrequency ultrasonic wave can reduce the energy barrier between enzyme digestion reaction ground state and activated state, while can increase enzyme and bottom
The contact probability of thing, so as to improve enzymolysis efficiency, reduce enzymolysis time.
Preferably, multidimensional used is VA or VB1Or VB2Or VB6Or VB12Or VC or VD or VE or VK or calcium pantothenate or
At least five kinds of mixture of nicotinic acid or folic acid or biotin or inositol or microcrystalline cellulose；Multidimensional can improve the antibacterial of body
Resistance against diseases, improve immunocompetence, stress ability.
Preferably, more ore deposits used are MgSO4·7H2O and CuSO4·5H2O and FeSO4·H2O and ZnSO4·H2O with
MnSO4·H2O and CoCl2·H2O and Ca (IO3)2With Na2SeO3Mixture；More ore deposits are necessary to body supplement is grown
Middle trace element.
Preferably, enzyme preparation is pectase or dextranase or phytase or amylase or high activity zytase or fibre
Tie up plain enzyme or neutral proteinase；Digest and absorb rate of the fish to feed nutrient is improved, feed coefficient is reduced, it is rich to reduce water body
Nutrient laden, water pollution is reduced, reduce the incidence of disease of fish.
Preferably, growth accelerator be zeolite powder or medical stone powder or compound sodium humate or crotons lactones or citric acid or
Haw thorn extract or dandelion extract；Growth accelerator can stimulate fish internal system, regulation metabolism, improve feed
Utilization rate, improve healthy fish, improve the production performance of fish.
Preferably, mould inhibitor is calcium formate or calcium propionate or calcium iodate or dimethyl fumarate or sodium benzoate；It is mould proof
Agent effectively can prevent feed mold from causing, so as to prevent feed nutrient missing, palatability from declining, or even causing mould
Toxin poisoning.
Compared with prior art, the advantage of the invention is that：1）The main raw material(s) of feed is both from industrial and agricultural production
Waste products, carried out processing remove anti-nutrient substance after be used as feed, not only improve the economic value of material, reality
Show full effect to utilize, and protected environment, reduce the cost of processing fertilizer；2）Urease activity, trypsase in feed
The ANFs such as inhibiting factor, stachyose, raffinose are significantly reduced, and aflatoxins content is also effectively controlled, from
And the indigestion after fish edible bean cake and allergic reaction are reduced, efficiency of feed utilization is improved, the probability of fish diseases is reduced, carries
High breeding efficiency；3）Feed nutrition general equilibrium, not only grown necessary protein, amino acid, fat rich in fresh-water fishes
The nutrients such as fat acid, vitamin, mineral matter, and feed can stimulate the smell of fish, the sense of taste, vision, attract fish to gather
Collection promotes fish feed, improves food-intake around feed, can also stimulate fish internal system, regulation metabolism, carry
High efficiency of feed utilization, improve healthy fish, improve the production performance of fish, be that a kind of promotion fresh water comprehensively, efficiently, safe is supported
Grow the feed of fish growth.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
Promote the preparation method of cultured freshwater fish growth feed, comprise the following steps：
1）3 parts of Tea Polyphenols powder are dissolved among 60 parts of absolute ethyl alcohols, ultrasonic dissolution, add 8 parts of egg yolk lecithins and 2.0 parts of courages
Sterol mixed solution stirring in water bath dissolves, and it is 7.2 that above-mentioned lipid ethanol solution, which is rapidly injected to 80 parts of pH, with syringe
Na2HPO4-KH2PO4In buffer solution, keep pH constant, stirring hydration 15 minutes at a temperature of 35 DEG C, be through depressurizing rotary evaporation
Obtain tea polyphenol nano lipidosome；2）By 3 parts of Radix Codonopsis, 5 portions of honeysuckles, 6 parts of Divine Comedy, 6 parts of bighead atractylodes rhizomes, 3 parts of Radixs Astragali, 10 parts of mast powder
Broken machine crushes, and crosses 80 mesh sieves, compound Chinese medicinal preparation is made；Compound Chinese medicinal preparation is weighed, is wrapped with degreasing cotton yarn, adds 8 times
Distilled water, it is boiled after soaking 1 hour, it is cooked by slow fire 30 minutes, is filtered with gauze, filter residue is decocted again, and filtrate is mixed
Close, low temperature is concentrated into aqueous 35%, saved backup at 3 DEG C；3）It is miscible in the water of 20 times of weight with wheat bran powder, adjust pH
For 5.0, the cellulase of wheatfeed 2% is added, bifrequency ultrasound-assisted enzymolysis, supersonic frequency be 25kHz and 35kHz, ultrasonic
Intensity is 0.35w/cm2, digested 1 hour under the conditions of 50 DEG C, heat up enzyme deactivation terminating reaction, and enzymolysis modified dietary fiber is made；
4）By dregs of beans and water according to solid-liquid ratio 1:2（g/ml）Uniformly mixing, microwave treatment 2 minutes, connect under the conditions of microwave power 800W
Lactobacillus paracasei, Lactobacillus salivarius, the bacillus licheniformis hybrid bacterial strain of kind 4%, at a temperature of 39 DEG C, ferment 48 hours, hair
Bean cake powder is made in drying and crushing after the completion of ferment；5）Weigh 70 parts of organic fertilizer, 70 parts of modified bean pulp powder, modified 30 parts of wheat bran, fat
12 parts of plastid, 30 parts of fish guts oil, 35 parts of fish protein hydrolyzate, 8 parts of dextrin, 20 parts of alphalise starch, 5 parts of yeast cell wall, marine alga
Sour 3 parts of sodium, 0.8 part of fennel oil, 1.0 parts of functional polypeptide, 0.55 part of VA, 0.55 part of VB1,0.55 part of VB2, VB6 0.55
Part, 0.55 part of VB12,0.55 part of VC, 4.2 parts of more ore deposits, 1.3 parts of dextranase, 4 parts of zeolite powder, 3 parts of compound sodium humate, first
Sour 0.5 part of calcium is well mixed, crosses 60 mesh sieves after drying, is granulated the feed for producing and promoting cultured freshwater fish growth, particle diameter is
0.2mm；Promote the feed nutrition general equilibrium of cultured freshwater fish growth, not only grown necessary albumen rich in fresh-water fishes
The nutrients such as matter, amino acid, aliphatic acid, vitamin, mineral matter, and feed can stimulate the smell of fish, the sense of taste, regard
Feel, attract fish collection around feed, promote fish feed, improve food-intake, fish internal system can also be stimulated, adjusted
Section metabolism, improve efficiency of feed utilization, improve healthy fish, improve the production performances of fish, be it is a kind of comprehensively, efficiently, peace
The full feed for promoting cultured freshwater fish growth.
Implement 2：
Promote the preparation method of cultured freshwater fish growth feed, including：The preparation of liposome, the preparation of Chinese medicine preparation, wheat bran change
Property, fermented bean dregs, compounding, specifically include following steps：
1）The preparation of liposome：4 parts of Tea Polyphenols powder, 10 parts of egg yolk lecithins and 2.5 parts of cholesterol are dissolved in 75 parts of anhydrous second
Among alcohol, above-mentioned lipid ethanol solution is rapidly injected to the Na for being 7.5 to 100 parts of pH with syringe2HPO4-KH2PO4Buffer solution
In, keep pH constant, stirring hydration 25 minutes, obtain tea polyphenol nano lipidosome suspension, by suspension at a temperature of 38 DEG C
Residual ethanol is flung to through depressurizing rotary evaporation, produces tea polyphenol nano lipidosome；Tea Polyphenols have inoxidizability, detoxication,
Radiation resistance, and have the function that to remove free radical；The phospholipid liposome that this method makes is easy to the absorption of fish, and
Preparation method is simple, and the physics and chemistry processing containing potentially harmful substances such as ether is avoided in preparation process, is especially suitable for industrializing
Large-scale production；
2）The preparation of Chinese medicine preparation：By 5 parts of Radix Codonopsis, 6 portions of honeysuckles, 7 parts of Divine Comedy, 7 parts of bighead atractylodes rhizomes, 5 parts of Radixs Astragali, 15 parts of mast powder
Broken machine crushes, and crosses 90 mesh sieves, compound Chinese medicinal preparation is made；Compound Chinese medicinal preparation is weighed, is wrapped with degreasing cotton yarn, adds 10 times
Distilled water, it is boiled after soaking 2 hours, it is cooked by slow fire 45 minutes, is filtered with gauze, filter residue is decocted again, and filtrate is mixed
Close, low temperature is concentrated into aqueous 40%, saved backup at 5 DEG C；Chinese medicine preparation can strengthen the disease-resistant antibacterial ability of fish, improve machine
Body immunity, builds up health, and the taste of Chinese medicine preparation uniqueness is advantageous to strengthen the food calling ability of phagostimulant；
3）Bran modification：Miscible in the water of 25 times of weight with wheat bran powder, regulation pH is 6.0, adds the fibre of wheatfeed 4%
Plain enzyme, bifrequency ultrasound-assisted enzymolysis are tieed up, frequency is 28kHz and 45kHz, ultrasound intensity 0.45w/cm2, in 55 DEG C of conditions
Lower enzymolysis 3 hours, heat up enzyme deactivation terminating reaction, and enzymolysis modified dietary fiber is made；The dietary fiber molecular weight of modified wheat bran
Decline, the edibility of wheat bran is improved, suitably as aquatic animal feed；
4）Fermented bean dregs：By dregs of beans and water according to solid-liquid ratio 1:3（g/ml）Uniformly mixing, it is micro- under the conditions of microwave power 1000W
Ripple is handled 3 minutes, 6% Lactobacillus paracasei, Lactobacillus salivarius, bacillus licheniformis hybrid bacterial strain is inoculated with, in 40 DEG C of temperature
Under, ferment 72 hours, bean cake powder is made in drying and crushing after the completion of fermentation；The urase that bean pulp fermentation can be reduced effectively in powder is lived
Property, the ANFs such as trypsin ihhibitor, stachyose, raffinose, aflatoxins pollution is reduced, so as to reduce fish food
With the indigestion after dregs of beans and allergic reaction, efficiency of feed utilization is improved, reduces the probability of fish diseases, improves breeding efficiency；
5）Compounding：Weigh 80 parts of organic fertilizer, 80 parts of modified bean pulp powder, modified 35 parts of wheat bran, 15 parts of liposome, fish guts oil 35
Part, 40 parts of fish protein hydrolyzate, 11 parts of dextrin, 25 parts of alphalise starch, yeast cell wall 5-6 parts, sodium alginate 3-4 parts, fennel oil
1.0 parts, 1.2 parts of functional polypeptide, 0.5 part of VA, 0.5 part of calcium pantothenate, 0.5 part of nicotinic acid, 0.5 part of folic acid, 0.5 part of biotin, flesh
0.5 part of alcohol, 0.5 part of microcrystalline cellulose, 4.5 parts of more ore deposits, 0.7 part of amylase, 0.7 part of cellulase, 4 parts of crotons lactones, lemon
4 parts of acid, 0.8 part of calcium propionate are well mixed, cross 60-80 mesh sieves after drying, are granulated the feed for producing and promoting cultured freshwater fish growth,
Particle diameter is 0.6mm；The amino acid sequence of functional polypeptide is：SCASRCCRARCGKSRCRARRCRARRCGCRARRCGYYVSVF
RC；Functional polypeptide can stimulate the liver and enteron aisle of fish, promote the secretion of fatty,fiss enzyme and amylase, improve the work of enzyme
Property, promote feed of the fish to feed and digest and assimilate, promote growth, put on weight, shorten the culture-cycle, reduce cultivate into
This；The main raw material(s) of feed is carried out removing anti-nutrient substance in processing both from the waste products of industrial and agricultural production
Feed is used as afterwards, not only improves the economic value of material, full effect is realized and utilizes, and protects environment, reduces processing
The cost of fertilizer.
Implement 3：
Promote the preparation method of cultured freshwater fish growth feed, including：The preparation of liposome, the preparation of Chinese medicine preparation, wheat bran change
Property, fermented bean dregs, compounding, specifically include following steps：
The preparation of liposome：4 parts of Tea Polyphenols powder are dissolved among 65 parts of absolute ethyl alcohols, ultrasonic dissolution, add 10 parts of yolk ovum
Above-mentioned lipid ethanol solution, is rapidly injected to 90 by phosphatide and 2.2 parts of cholesterol mixed solution stirring in water bath dissolvings with syringe
The Na that part pH is 7.42HPO4-KH2PO4In buffer solution, keep pH constant, stirring hydration 20 minutes, obtain tea at a temperature of 37 DEG C
Polyphenol nano-lipid liquid suspension, suspension is flung into residual ethanol through depressurizing rotary evaporation, produces tea polyphenol nano lipidosome；
Tea Polyphenols has inoxidizability, detoxication, radiation resistance, and has the function that to remove free radical；What this method made
Phospholipid liposome is easy to the absorption of fish, and preparation method is simple, is avoided in preparation process potentially harmful containing ether etc.
The physics and chemistry processing of material, is especially suitable for industrialization large-scale production；
The preparation of Chinese medicine preparation：By 4 parts of Radix Codonopsis, 5 portions of honeysuckles, 6 parts of Divine Comedy, 7 parts of bighead atractylodes rhizomes, 4 parts of Radixs Astragali, the crushing of 12 parts of masts
Machine crushes, and crosses 80 mesh sieves, compound Chinese medicinal preparation is made；Compound Chinese medicinal preparation is weighed, is wrapped with degreasing cotton yarn, adds 9 times of steaming
Distilled water, it is boiled after soaking 1.5 hours, it is cooked by slow fire 40 minutes, is filtered with gauze, filter residue is decocted again, and filtrate is mixed
Close, low temperature is concentrated into aqueous 35%, saved backup at 4 DEG C；Chinese medicine preparation can strengthen the disease-resistant antibacterial ability of fish, improve machine
Body immunity, builds up health, and the taste of Chinese medicine preparation uniqueness is advantageous to strengthen the food calling ability of phagostimulant；
Bran modification：Miscible in the water of 22 times of weight with wheat bran powder, regulation pH is 5.5, adds the fiber of wheatfeed 3%
Plain enzyme, bifrequency ultrasound-assisted enzymolysis, frequency are 26kHz and 40kHz, ultrasound intensity 0.40w/cm2, under the conditions of 52 DEG C
Enzymolysis 2 hours, heat up enzyme deactivation terminating reaction, and enzymolysis modified dietary fiber is made；Enzymolysis modified bran dietary fiber concave-convex surface
Injustice, retention ability, expansive force, dissolubility and the viscosity of modified dietary fiber and to fat, cholesterol, nitrite ion
Adsorption capacity all accordingly improve, the dietary fiber molecular weight and molecular weight of modified wheat bran, improve the edibility of wheat bran, suitable conduct
Aquatic animal feed；
Fermented bean dregs：By dregs of beans and water according to solid-liquid ratio 1:3（g/ml）Uniformly mixing, under the conditions of microwave power 900W at microwave
Reason 2 minutes, 5% Lactobacillus paracasei, Lactobacillus salivarius, bacillus licheniformis hybrid bacterial strain are inoculated with, at a temperature of 40 DEG C, hair
Ferment 60 hours, bean cake powder is made in drying and crushing after the completion of fermentation；Bean pulp fermentation can be reduced effectively to urease activity, the pancreas in powder
The ANFs such as protease inhibitor, stachyose, raffinose, aflatoxins pollution is reduced, beans are eaten so as to reduce fish
Indigestion and allergic reaction after the dregs of rice, efficiency of feed utilization is improved, reduce the probability of fish diseases, improve breeding efficiency；
Compounding：Weigh 75 parts of organic fertilizer, 75 parts of modified bean pulp powder, modified 32 parts of wheat bran, 14 parts of liposome, fish guts oil 32
Part, 38 parts of fish protein hydrolyzate, 10 parts of dextrin, 24 parts of alphalise starch, 5 parts of yeast cell wall, 4 parts of sodium alginate, fennel oil 0.9
Part, 1.1 parts of functional polypeptide, 0.5 part of VB6,0.5 part of VB12,0.5 part of VC, 0.5 part of VD, 0.5 part of VE, 0.5 part of VK,
0.4 part of calcium pantothenate, 4.4 parts of more ore deposits, 0.3 part of phytase, 0.5 part of amylase, 0.5 part of neutral proteinase, 2 parts of crotons lactones, mountain
3 parts of short, bristly hair or beard extract, 2.5 parts of medical stone powder, 0.6 part of sodium benzoate are well mixed, and 80 mesh sieves are crossed after drying, be granulated produce promote it is light
The feed of water cultivation fish growth, particle diameter 0.5mm；The amino acid sequence of functional polypeptide is：SCASRCCRARCGKSRCRARRC
RARRCGCRARRCGYYVSVFRC；Functional polypeptide can stimulate the liver and enteron aisle of fish, promote fatty,fiss enzyme and starch
The secretion of enzyme, the activity of enzyme is improved, promote feed of the fish to feed and digest and assimilate, promoted growth, put on weight, shorten and support
Grow the cycle, reduce aquaculture cost；The main raw material(s) of feed both from the waste products of industrial and agricultural production adding
Work is used as feed after removing anti-nutrient substance, not only improves the economic value of material, realizes full effect and utilizes, and protects
Environment, reduce the cost of processing fertilizer；Promote the feed nutrition general equilibrium of cultured freshwater fish growth, not only rich in fresh-water fishes
Grow the nutrients such as necessary protein, amino acid, aliphatic acid, vitamin, mineral matter, and feed can stimulate
The smell of fish, the sense of taste, vision, attract fish collection around feed, promote fish feed, improve food-intake, can also pierce
Swash fish internal system, regulation metabolism, improve efficiency of feed utilization, improve healthy fish, improve the production performance of fish,
It is a kind of feed for promoting cultured freshwater fish growth comprehensively, efficiently, safe.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
Sequence table
<110>Ningbo City Yinzhou District Chao Rui food science and technologies Co., Ltd
<120>Promote the preparation method of cultured freshwater fish growth feed
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 42
<212> PRT
<213>Artificial synthesized (Mytilus coruscus)
<400> 1
Ser Cys Ala Ser Arg Cys Cys Arg Ala Arg Cys Gly Lys Ser Arg Cys
1 5 10 15
Arg Ala Arg Arg Cys Arg Ala Arg Arg Cys Gly Cys Arg Ala Arg Arg
20 25 30
Cys Gly Tyr Tyr Val Ser Val Phe Arg Cys
35 40
Claims (9)
1. promote the preparation method of cultured freshwater fish growth feed, including：The preparation of liposome, the preparation of Chinese medicine preparation, wheat bran
Modification, fermented bean dregs, compounding, it is characterised in that：Described bran modification step is：Wheat bran powder is mixed soluble in water, added
Enter wheatfeed cellulase, bifrequency ultrasound-assisted enzymolysis digests 1-3 hours under the conditions of 50-55 DEG C, and heating enzyme deactivation terminates
Reaction, enzymolysis modified dietary fiber is made.
2. the preparation method according to claim 1 for promoting cultured freshwater fish growth feed, it is characterised in that：The East Sea
The preparation process of liposome is：3-4 part Tea Polyphenols powder is dissolved among 60-75 part absolute ethyl alcohols, ultrasonic dissolution, adds 8-10
Part egg yolk lecithin and the dissolving of 2.0-2.5 part cholesterol mixed solutions stirring in water bath, with syringe by above-mentioned lipid ethanol solution
It is rapidly injected to the Na that 80-100 parts pH is 7.2-7.52HPO4-KH2PO4In buffer solution, keep pH constant, in 35-38 DEG C of temperature
Lower stirring hydration 15-25 minutes, tea polyphenol nano lipidosome suspension is obtained, suspension is flung into residual through depressurizing rotary evaporation
Ethanol, produce tea polyphenol nano lipidosome.
3. the preparation method according to claim 1 for promoting cultured freshwater fish growth feed, it is characterised in that：In described
The preparation steps of medicine preparation are：By 3-5 parts Radix Codonopsis, 5-6 parts honeysuckle, 6-7 parts Divine Comedy, 6-7 parts bighead atractylodes rhizome, 3-5 parts Radix Astragali, 10-
15 parts of masts are crushed with pulverizer, cross 80-90 mesh sieves, compound Chinese medicinal preparation is made；Compound Chinese medicinal preparation is weighed, with degreasing cotton yarn
Wrap, add 8-10 times of distilled water, it is boiled after immersion 1-2 hours, 30-45 minutes are cooked by slow fire, are filtered with gauze, are filtered
Slag is decocted again, and filtrate is mixed, and low temperature is concentrated into aqueous 35-40%, is saved backup at 3-5 DEG C.
4. the preparation method according to claim 1 for promoting cultured freshwater fish growth feed, it is characterised in that：Described bran
Skin modification procedure is：Wheat bran powder is miscible in the water of 20-25 times of weight, and regulation pH is 5.0-6.0, adds wheatfeed 2-
4% cellulase, bifrequency ultrasound-assisted enzymolysis digest 1-3 hours under the conditions of 50-55 DEG C, and heating enzyme deactivation terminates anti-
Should, enzymolysis modified dietary fiber is made.
5. the preparation method according to claim 4 for promoting cultured freshwater fish growth feed, it is characterised in that：Described bran
The frequency of bifrequency ultrasonic wave in skin modification procedure is 25-28kHz and 35-45kHz, ultrasound intensity 0.35-0.45w/
cm2。
6. the preparation method according to claim 1 for promoting cultured freshwater fish growth feed, it is characterised in that：Described hair
Ferment dregs of beans step is：By dregs of beans and water according to solid-liquid ratio 1:2-3（g/ml）Uniformly mixing, in microwave power 800-1000W conditions
Lower microwave treatment 2-3 minutes, 4-6% Lactobacillus paracasei, Lactobacillus salivarius, bacillus licheniformis hybrid bacterial strain are inoculated with,
At a temperature of 39-40 DEG C, ferment 48-72 hours, bean cake powder is made in drying and crushing after the completion of fermentation.
7. the preparation method according to claim 1 for promoting cultured freshwater fish growth feed, it is characterised in that：Described answers
It is with step：Weigh organic fertilizer 70-80 parts, modified bean pulp powder 70-80 parts, modified wheat bran 30-35 parts, liposome 12-15 parts,
Fish guts oil 30-35 parts, fish protein hydrolyzate 35-40 parts, dextrin 8-11 parts, alphalise starch 20-25 parts, yeast cell wall 5-6 parts,
Sodium alginate 3-4 parts, fennel oil 0.8-1.0 parts, functional polypeptide 1.0-1.2 parts, multidimensional 3.2-3.5 parts, more ore deposit 4.2-4.5
Part, enzyme preparation 1.3-1.4 parts, growth accelerator 7-8 parts, preservative 0.5-0.8 parts are well mixed, and cross 60-80 mesh sieves after drying, make
Grain produces the feed for promoting cultured freshwater fish growth, particle diameter 0.2-0.6mm.
8. the preparation method according to claim 7 for promoting cultured freshwater fish growth feed, it is characterised in that：The compounding
The amino acid sequence of functional polypeptide in step is：SCASRCCRARCGKSRCRARRCRARRCGCRARRCGYYVSVFRC.
9. the preparation method according to claim 7 for promoting cultured freshwater fish growth feed, it is characterised in that：The compounding
Multidimensional in step is VA or VB1Or VB2Or VB6Or VB12Or VC or VD or VE or VK or calcium pantothenate or nicotinic acid or folic acid or biology
Element or at least five kinds of mixture of inositol or microcrystalline cellulose, more ore deposits are MgSO4·7H2O and CuSO4·5H2O and FeSO4·
H2O and ZnSO4·H2O and MnSO4·H2O and CoCl2·H2O and Ca (IO3)2With Na2SeO3Mixture, enzyme preparation is pectase
Or dextranase or phytase or amylase or high activity zytase or cellulase or neutral proteinase, growth accelerator are boiling
Stone flour or medical stone powder or compound sodium humate or crotons lactones or citric acid or haw thorn extract or dandelion extract, it is mould proof
Agent is calcium formate or calcium propionate or calcium iodate or dimethyl fumarate or sodium benzoate.
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| CN201710950838.0A CN107647113A (en) | 2017-10-13 | 2017-10-13 | Promote the preparation method of cultured freshwater fish growth feed |
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| CN201710950838.0A CN107647113A (en) | 2017-10-13 | 2017-10-13 | Promote the preparation method of cultured freshwater fish growth feed |
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| CN201710950838.0A Withdrawn CN107647113A (en) | 2017-10-13 | 2017-10-13 | Promote the preparation method of cultured freshwater fish growth feed |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108477430A (en) * | 2018-02-09 | 2018-09-04 | 广西平南县平原农牧有限公司 | A kind of spot Of-digestive-tract food calling nourishing additive agents |
| CN108611298A (en) * | 2018-05-05 | 2018-10-02 | 上海海洋大学 | A kind of deep-sea bacillus and its application in induction Trachyostracous mussel juvenile mollusk attachment |
| CN109221824A (en) * | 2018-11-07 | 2019-01-18 | 南京农业大学 | A kind of efficient aquatic products glycometabolism regulatory composition and the preparation method and application thereof |
| CN112617008A (en) * | 2020-12-29 | 2021-04-09 | 西藏自治区农牧科学院水产科学研究所 | Feed for wild schizothorax prenanti seedlings |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997008959A1 (en) * | 1995-09-01 | 1997-03-13 | Research Institute For Production Development | Feed for fishes and shellfishes |
| CN101461534A (en) * | 2009-01-14 | 2009-06-24 | 江南大学 | Method for preparing antioxidation gelatine membrane containing tea polyphenol nano lipidosome |
| CN101731475A (en) * | 2009-12-11 | 2010-06-16 | 华中农业大学 | Compound feed suitable for juvenile fish of myxocyprinus asiaticus |
| CN106578947A (en) * | 2016-12-21 | 2017-04-26 | 安徽豆宝食品有限公司 | Spleen invigorating rose hip and coix seed instant powder and production method thereof |
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2017
- 2017-10-13 CN CN201710950838.0A patent/CN107647113A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997008959A1 (en) * | 1995-09-01 | 1997-03-13 | Research Institute For Production Development | Feed for fishes and shellfishes |
| CN101461534A (en) * | 2009-01-14 | 2009-06-24 | 江南大学 | Method for preparing antioxidation gelatine membrane containing tea polyphenol nano lipidosome |
| CN101731475A (en) * | 2009-12-11 | 2010-06-16 | 华中农业大学 | Compound feed suitable for juvenile fish of myxocyprinus asiaticus |
| CN106578947A (en) * | 2016-12-21 | 2017-04-26 | 安徽豆宝食品有限公司 | Spleen invigorating rose hip and coix seed instant powder and production method thereof |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108477430A (en) * | 2018-02-09 | 2018-09-04 | 广西平南县平原农牧有限公司 | A kind of spot Of-digestive-tract food calling nourishing additive agents |
| CN108611298A (en) * | 2018-05-05 | 2018-10-02 | 上海海洋大学 | A kind of deep-sea bacillus and its application in induction Trachyostracous mussel juvenile mollusk attachment |
| CN108611298B (en) * | 2018-05-05 | 2021-03-23 | 上海海洋大学 | Deep sea bacillus and application thereof in inducing juvenile mytilus coruscus to attach |
| CN109221824A (en) * | 2018-11-07 | 2019-01-18 | 南京农业大学 | A kind of efficient aquatic products glycometabolism regulatory composition and the preparation method and application thereof |
| CN109221824B (en) * | 2018-11-07 | 2021-12-21 | 南京农业大学 | Aquatic sugar metabolism regulation and control composition and preparation method and application thereof |
| CN112617008A (en) * | 2020-12-29 | 2021-04-09 | 西藏自治区农牧科学院水产科学研究所 | Feed for wild schizothorax prenanti seedlings |
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