CN107646237A - A kind of method for culturing seedlings for breaking pears seed dormancy - Google Patents
A kind of method for culturing seedlings for breaking pears seed dormancy Download PDFInfo
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- CN107646237A CN107646237A CN201711022632.8A CN201711022632A CN107646237A CN 107646237 A CN107646237 A CN 107646237A CN 201711022632 A CN201711022632 A CN 201711022632A CN 107646237 A CN107646237 A CN 107646237A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Soil Sciences (AREA)
- Inorganic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of method for breaking pears seed dormancy, the step of mainly including seed selection, the immersion of seed immersion treatment, plant leaching liquor immersion and Stratificated treatment, present invention comprehensive utilization immersion, Stratificated treatment etc. break the technology of seed dormancy, substantially reduce the time of pears seed sprouting, and the seedling from seed well developed root system of the pears obtained using the present invention, and growing way is good, speed is fast, and survival rate is high.
Description
Technical field
The invention belongs to plant biotechnology field, and in particular to a kind of method for culturing seedlings for breaking pears seed dormancy.
Background technology
Pears are rose family Pyrus L, and perennial deciduous fruit tree, arbor, leaf is avette, spend it is more white, it is fruit succulence, edible.
Pears are known as the good reputation of " ancestors of all kinds of fruits ", because of its fresh and tender succulence, sour and sweet palatability, so having the title of " natural mineral water " again, in the world
In fruit market, it is referred to as " three big fruits seize by force " with apple and orange.Cultivation mainly has Ussurian pear, white pear, sand pear, the foreign pears four to be
System.Pear cultivation is with a long history, and species is wide in variety, strong adaptability, easily high and stable yields, deep to be welcome by the masses.
Seed dormancy is a normal physiological phenomena of plant growth and development process, is plant to environmental condition and seasonality
Change the adaptability of biology.Pears seed generally existing dormancy.Kind skin obstacle, kind embryo dormancy and kind embryo after-ripening etc. are to cause
The main reason for pears seed dormancy.In the practices of breeding, need to be by the real ability after storing certain time under cryogenic of allocarpy
It is set to complete the after-ripening of embryo.Even if so, still suffer from the problems such as hybrid seed emergence rate is low, emergence is irregular.Currently, lead to
Improved Varieties pears of the selection cross acquisition with characteristics such as high yield, disease and insect resistance, anti-extraneous bad conditions are crossed to be continuously increased.But
In crossbreeding, if seed dormancy can not be released in time, often there is the low situation of germination percentage.
Seed dormancy is typically released using the method for sand Tibetan at present, but this method, which easily causes germination, prolongs
Late, and seed is easily mouldy during Sha Zang.Measure, the complexity such as use also is peeled off, peeling are taken a lot of work, and have had a strong impact on breeding
Operating efficiency.Therefore the processing method of more preferably releasing seed dormancy is sought, to shorten the time required to pears seed is sprouted and improve
Germination rate, pear cultivation nursery and breeding tool are of great significance.
The content of the invention
In order to solve above-mentioned technical problem present in prior art, the present invention provides a kind of
The present invention provides a kind of method for breaking pears seed dormancy for existing deficiency.
Above-mentioned purpose is realized by the following method in the present invention.
A kind of method for breaking pears seed dormancy, comprises the following steps：
(1) seed collection：The ripe, pears without insect pest are chosen, pears are placed under conditions of temperature is 25 DEG C and stacked 5~7 days,
Then seed is taken out from pears, is dried, full seed is filtered out and fully ripe seed is standby；
(2) after pears seed first being soaked into 13~17h with potassium dihydrogen phosphate, it is put into 600 times of liquid of thiophanate methyl and soaks
Bubble 2 hours, finally cleans seed with 30 DEG C or so of warm water；
(3) plant leaching liquor immersion is prepared：Root, stem, the leaf of vetiver are gathered, leaching liquor I is prepared into, is added in leaching liquor I
Gibberellin and salicylic acid obtain leaching liquor II, and the seed that step (2) is obtained soaks 8~10h in leaching liquor II；
(4) mixed with sand, vermiculite, perlite with the seed that step (3) obtains and load seed packet, then put seed packet
Enter in insulating box, be 23~25 DEG C, Stratificated treatment 3-5d by temperature setting, during which spray a Bravo 700 to matrix daily
Times liquid；
Potassium dihydrogen phosphate concentration described in step (2) is 30g/L~40g/L.
The preparation method of leaching liquor I described in step (3) is root, stem, the leaf of the healthy and strong vetiver of collection health, after drying in the shade
Shredded with scissors, weigh totally 16 parts of root, stem, leaf, add 100 times of distilled water, 25~30 DEG C are soaked 48h, are obtained after 4 layers of filtered through gauze
Leaching liquor stoste, then concentrate is evaporated under reduced pressure to obtain with Rotary Evaporators, 100ml is settled to, produces leaching liquor I；
Cus-cus grass roots, stem, leaf parts by weights ratio described in step (3) are：1：5：2.
Gibberellins solution concentration described in step (3) is 21~23ppm.
Salicylic acid solution concentration described in step (3) is 16~18ppm.
The mixing quality ratio of each composition is sand in step (4) described Stratificated treatment:Vermiculite:Perlite:Seed=
3:2:1:1。
Step (4) described soil humidity is maintained at 65~75%.
Beneficial effect：
1st, present invention comprehensive utilization immersion, growth regulator control, plant extraction liquid and Stratificated treatment etc. break seed
The technology of dormancy, and the seedling from seed well developed root system of the pears obtained using the present invention, and growing way is good, and speed is fast, survives
Rate is high.
2nd, vetiver contains protein, amino acid and lipid needed for animal, mineral element etc., wherein, vetiver is thick
Protein content is 7.31%, crude fat 1.97%, crude fibre 34.5%, and the nutrition for providing abundance can be sprouted for seed, is used
After containing vetiver leaching liquor and growth regulator, salicylic immersion, the sprouting survival rate of pears seed is greatly improved.
3rd, present invention employs Stratificated treatment, including the matrix of Stratificated treatment is reasonably configured, wherein perlite
The gas permeability of matrix is ensure that with river sand, and sand then maintains sufficient moisture for matrix, it is final to improve using above-mentioned setting
The emergence rate of pears, ensure that the uniformity of emergence.
Embodiment
Limited with reference to specific embodiment technical scheme is further, but claimed
Scope is not only limited to made description.
Embodiment one
A kind of method for breaking pears seed dormancy, it is characterised in that this method comprises the following steps：
(1) seed collection：The ripe, pears without insect pest are chosen, pears are placed on temperature to stack 6 days under conditions of 25 DEG C, so
Seed is taken out from pears afterwards, is dried, full seed is filtered out and fully ripe seed is standby；
(2) after being first 35g/L potassium dihydrogen phosphates immersion 15h with concentration by pears seed, it is put into 600 times of thiophanate methyl
Soaked 2 hours in liquid, finally clean seed with 30 DEG C or so of warm water；
(3) plant leaching liquor immersion is prepared：Well-developed plant is chosen, gathers the root, stem, leaf of vetiver, with cutting after drying in the shade
Knife is shredded, and weighs root, stem, leaf 2,10,4g respectively, adds 100 times of distilled water, and 28 DEG C are soaked 48h, must be soaked after 4 layers of filtered through gauze
Extract stoste, then concentrate is evaporated under reduced pressure to obtain with Rotary Evaporators, it is settled to 100ml；In above-mentioned middle addition 22ppm gibberellin and
17ppm salicylic acids obtain leaching liquor, and the seed that step (2) is obtained soaks 9h in above-mentioned leaching liquor；
(4) mixed with sand, vermiculite, perlite with the seed that step (3) obtains and load seed packet, then put seed packet
Enter in insulating box, be 24 DEG C, Stratificated treatment 4d by temperature setting, during which spray 700 times of liquid of a Bravo daily, for matrix
While sterilization, soil humidity is set to be maintained at 70% or so；The mixing quality ratio of each composition is sand in the Stratificated treatment
Son:Vermiculite:Perlite:Seed=3:2:1:1.
Embodiment two
A kind of method for breaking pears seed dormancy, it is characterised in that this method comprises the following steps：
(1) seed collection：The ripe, pears without insect pest are chosen, pears are placed on temperature to stack 7 days under conditions of 25 DEG C, so
Seed is taken out from pears afterwards, is dried, full seed is filtered out and fully ripe seed is standby；
(2) after being first 40g/L potassium dihydrogen phosphates immersion 17h with concentration by pears seed, it is put into 600 times of thiophanate methyl
Soaked 2 hours in liquid, finally clean seed with 30 DEG C or so of warm water；
(3) plant leaching liquor immersion is prepared：Well-developed plant is chosen, gathers the root, stem, leaf of vetiver, with cutting after drying in the shade
Knife is shredded, and weighs root, stem, leaf 2,10,4g respectively, adds 100 times of distilled water, and 30 DEG C are soaked 48h, must be soaked after 4 layers of filtered through gauze
Extract stoste, then concentrate is evaporated under reduced pressure to obtain with Rotary Evaporators, it is settled to 100ml；In above-mentioned middle addition 23ppm gibberellin and
18ppm salicylic acids obtain leaching liquor, and the seed that step (2) is obtained soaks 10h in above-mentioned leaching liquor；
(4) mixed with sand, vermiculite, perlite with the seed that step (3) obtains and load seed packet, then put seed packet
Enter in insulating box, be 25 DEG C, Stratificated treatment 5d by temperature setting, during which spray 700 times of liquid of a Bravo daily, for matrix
While sterilization, soil humidity is set to be maintained at 75% or so；The mixing quality ratio of each composition is sand in the Stratificated treatment
Son:Vermiculite:Perlite:Seed=3:2:1:1.
Embodiment three
A kind of method for breaking pears seed dormancy, it is characterised in that this method comprises the following steps：
(1) seed collection：The ripe, pears without insect pest are chosen, pears are placed on temperature to stack 5 days under conditions of 25 DEG C, so
Seed is taken out from pears afterwards, is dried, full seed is filtered out and fully ripe seed is standby；
(2) after being first 30g/L potassium dihydrogen phosphates immersion 13h with concentration by pears seed, it is put into 600 times of thiophanate methyl
Soaked 2 hours in liquid, finally clean seed with 30 DEG C or so of warm water；
(3) plant leaching liquor immersion is prepared：Well-developed plant is chosen, gathers the root, stem, leaf of vetiver, with cutting after drying in the shade
Knife is shredded, and weighs root, stem, leaf 2,10,4g respectively, adds 100 times of distilled water, and 25 DEG C are soaked 48h, must be soaked after 4 layers of filtered through gauze
Extract stoste, then concentrate is evaporated under reduced pressure to obtain with Rotary Evaporators, it is settled to 100ml；In above-mentioned middle addition 21ppm gibberellin and
16ppm salicylic acids obtain leaching liquor, and the seed that step (2) is obtained soaks 8h in above-mentioned leaching liquor；
(4) mixed with sand, vermiculite, perlite with the seed that step (3) obtains and load seed packet, then put seed packet
Enter in insulating box, be 23 DEG C, Stratificated treatment 3d by temperature setting, during which spray 700 times of liquid of a Bravo daily, for matrix
While sterilization, soil humidity is set to be maintained at 65% or so；The mixing quality ratio of each composition is sand in the Stratificated treatment
Son:Vermiculite:Perlite:Seed=3:2:1:1.
By measure, after the processing of embodiment 1,2,3, the germination rate of pears has respectively reached 95%, 93%, 94%.
Claims (8)
- A kind of 1. method for breaking pears seed dormancy, it is characterised in that this method comprises the following steps：(1) seed collection：The ripe, pears without insect pest are chosen, pears are placed on temperature to stack 5~7 days under conditions of 25 DEG C, then Seed is taken out from pears, is dried, full seed is filtered out and fully ripe seed is standby；(2) after pears seed first being soaked into 13~17h with potassium dihydrogen phosphate, it is small to be put into immersion 2 in 600 times of liquid of thiophanate methyl When, finally clean seed with 30 DEG C or so of warm water；(3) plant leaching liquor immersion is prepared：Root, stem, the leaf of vetiver are gathered, leaching liquor I is prepared into, is added in leaching liquor I red mould Element and salicylic acid obtain leaching liquor II, and the seed that step (2) is obtained soaks 8~10h in leaching liquor II；(4) mixed with sand, vermiculite, perlite with the seed that step (3) obtains and load seed packet, seed packet is then put into perseverance In incubator, it is 23~25 DEG C, Stratificated treatment 3-5d by temperature setting, during which sprays 700 times of liquid of a Bravo to matrix daily.
- 2. the method as claimed in claim 1 for breaking pears seed dormancy, it is characterised in that the biphosphate described in step (2) Potassium solution concentration is 30g/L~40g/L.
- 3. the as claimed in claim 1 method for breaking pears seed dormancy, it is characterised in that leaching liquor I described in step (3) Preparation method is root, stem, the leaf of the healthy and strong vetiver of collection health, to be shredded after drying in the shade with scissors, weigh totally 16 parts of root, stem, leaf, 100 times of distilled water are added, 25~30 DEG C are soaked 48h, obtain leaching liquor stoste after 4 layers of filtered through gauze, then depressurized with Rotary Evaporators Concentrate is evaporated to obtain, is settled to 100ml, produces leaching liquor I.
- 4. the as claimed in claim 1 method for breaking pears seed dormancy, it is characterised in that cus-cus grass roots described in step (3), Stem, leaf parts by weights ratio are：1：5：2.
- 5. the method as claimed in claim 1 for breaking pears seed dormancy, it is characterised in that the gibberellin described in step (3) is molten Liquid concentration is 21~23ppm.
- 6. the method as claimed in claim 1 for breaking pears seed dormancy, it is characterised in that the salicylic acid described in step (3) is molten Liquid concentration is 16~18ppm.
- 7. the method as claimed in claim 1 for breaking pears seed dormancy, it is characterised in that in step (4) described Stratificated treatment The mixing quality ratio of each composition is sand:Vermiculite:Perlite:Seed=3:2:1:1.
- 8. the as claimed in claim 1 method for breaking pears seed dormancy, it is characterised in that step (4) described soil humidity is 65~75%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201711022632.8A CN107646237A (en) | 2017-10-26 | 2017-10-26 | A kind of method for culturing seedlings for breaking pears seed dormancy |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711022632.8A CN107646237A (en) | 2017-10-26 | 2017-10-26 | A kind of method for culturing seedlings for breaking pears seed dormancy |
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| CN107646237A true CN107646237A (en) | 2018-02-02 |
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| CN201711022632.8A Pending CN107646237A (en) | 2017-10-26 | 2017-10-26 | A kind of method for culturing seedlings for breaking pears seed dormancy |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108323274A (en) * | 2018-04-04 | 2018-07-27 | 徐丽 | A method of abolishing lady's-grass seed dormancy |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430656A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Seedling culture method capable of relieving pear seeds from dormancy |
| CN104365213A (en) * | 2014-09-12 | 2015-02-25 | 陈小洁 | Method for breaking thalictrum aquilegifolium seed dormancy |
| US20160037772A1 (en) * | 2014-08-08 | 2016-02-11 | Mario Miguel Guerrero Mendez | Increasing abiotic stress tolerance in plants |
| CN106973581A (en) * | 2017-04-07 | 2017-07-25 | 扬州大学 | A kind of method for releasing pears seed dormancy |
| CN107114029A (en) * | 2017-04-07 | 2017-09-01 | 扬州大学 | A kind of method of quickly breaking dormancy of birch-leaf pear seeds |
-
2017
- 2017-10-26 CN CN201711022632.8A patent/CN107646237A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103430656A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Seedling culture method capable of relieving pear seeds from dormancy |
| US20160037772A1 (en) * | 2014-08-08 | 2016-02-11 | Mario Miguel Guerrero Mendez | Increasing abiotic stress tolerance in plants |
| CN104365213A (en) * | 2014-09-12 | 2015-02-25 | 陈小洁 | Method for breaking thalictrum aquilegifolium seed dormancy |
| CN106973581A (en) * | 2017-04-07 | 2017-07-25 | 扬州大学 | A kind of method for releasing pears seed dormancy |
| CN107114029A (en) * | 2017-04-07 | 2017-09-01 | 扬州大学 | A kind of method of quickly breaking dormancy of birch-leaf pear seeds |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108323274A (en) * | 2018-04-04 | 2018-07-27 | 徐丽 | A method of abolishing lady's-grass seed dormancy |
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