CN107643400A - The preparation method and applications of cancer markers detection architecture based on silver-colored aggregation polymer and Crystal structure - Google Patents
The preparation method and applications of cancer markers detection architecture based on silver-colored aggregation polymer and Crystal structure Download PDFInfo
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- CN107643400A CN107643400A CN201710804383.1A CN201710804383A CN107643400A CN 107643400 A CN107643400 A CN 107643400A CN 201710804383 A CN201710804383 A CN 201710804383A CN 107643400 A CN107643400 A CN 107643400A
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Abstract
The invention discloses the preparation method and applications based on silver-colored aggregation polymer and the cancer markers detection architecture of Crystal structure, feature is to comprise the following steps：（1）The preparation of silver-colored aggregation polymer SERS immunological probes；（2）The preparation of substrate is immunized in Crystal structure；（3）Cancer markers detection architecture assembles；Its application is that the cancer markers detection architecture based on silver-colored aggregation polymer and Crystal structure is carried out into spectral measurement using Raman spectrometer, according to the quantitative relationship between spectral intensity and antigen concentration, the concentration for obtaining antigen in the phosphate buffer solution containing determined antigen can be calculated, advantage is the preparation method and applications of the cancer markers detection architecture based on silver-colored aggregation polymer and Crystal structure of specificity and repeatability that testing result is improved while relatively low detectable limit is obtained.
Description
Technical field
The present invention relates to a kind of detection method of cancer markers, and silver-colored aggregation-polymer is based on more particularly, to one kind
With the preparation method and applications of the cancer markers detection architecture of Crystal structure.
Background technology
In recent years, as the continuous quickening of human being's production rhythm of life, global warming, bio-diversity fall sharply, area
Disease problems caused by the environmental disruptions such as domain property air combined pollution are more serious.Research shows more than 70% carcinogenic and rush
Cancer factor and environmental pollution close relation.With the progress of photoelectron science and technology, particularly by micro-nano preparation skill
Art, advanced photoelectric detecting technology are combined with modern biotechnology, and people have been developed based on the Gao Te between antigen-antibody
The biochip of specific immunological reaction detection cancer-specific antigen.At present, the immune inspection of SERS (SERS) base
One study hotspot and difficult point in survey field are how to obtain further to obtain the high signal output of intensity, and then are obtained relatively low
The immune detection limit.Research shows, to obtain the relatively low SERS base immune detection limit, need to exist in immune substrate
Hot spot region with especially high electromagnetic field intensity.Therefore, it is controllable and simple how with advanced micro Nano material technology of preparing
Just synthesize the focus with high SERS enhancers, further promote SERS base immunoassay technologies mature still need to compared with
Big effort.The uniformity is higher especially by designing and producing, and signal intensity is larger and the immunological probe and substrate pair that last a long time
The clinical serum sample of cancer patient carries out immunoassays, while relatively low detectable limit is obtained, the spy that improves testing result
The opposite sex and repeatability, to improve the rehabilitation of cancer sufferer and chances of survival be still SERS base field of immunodetection difficult point
And major tasks.
The content of the invention
The technical problems to be solved by the invention are to provide one kind while relatively low detectable limit is obtained, and improve detection knot
The specificity of fruit and the cancer markers detection architecture based on silver-colored aggregation-polymer and Crystal structure of repeatability
Preparation method and applications.
Technical scheme is used by the present invention solves above-mentioned technical problem：One kind is based on silver-colored aggregation-polymer and gold
The preparation method of the cancer markers detection architecture of nano-wire array, comprises the following steps：
(1) preparation of silver-colored aggregation-polymer SERS immunological probes
A. by 1.47-4.41 milligrams sodium citrate and 1.97-5.91 milligram gold chlorides be dissolved in 20-60 milliliter water be made mixing it is molten
Liquid, the concentration for adding 0.6-1.8 milliliter Fresh when being stirred vigorously at room temperature is the sodium borohydride water of 4 mg/mls
Solution, obtain gold seeds solution；
B. 3-9 milliliter gold seeds solution is taken to be added in the aqueous solution of the 50-150 milliliters containing silver nitrate and sodium citrate, afterwards
The aqueous ascorbic acid that 10-30 milliliters concentration is 2 mg/mls is added dropwise thereto, reaction obtains silver nanoparticle in 1 hour
The grain aqueous solution；
C. it is 10-30 microlitres to take 1-3 milliliter silver nano-grain aqueous solution centrifugal concentrating, gradually adds 820-2460 microlitres thereto
Dimethylformamide, the dimethyl formamide solution and 20-60 microlitres of water of the 5-15 microlitres of thionaphthol containing 2-, it is subsequently placed in 60 DEG C of bakings
After being cultivated 3 hours in case, the 80-240 microlitres of diformazan containing polystyrene polyacrylic acid diblock copolymer is added thereto
Base formamide solution and 180-540 microlitres of water, it is subsequently placed in after being cultivated 2 hours in 110 DEG C of baking ovens and centrifuges, obtained sediment is i.e.
It is for silver-colored aggregation-polymer, the buffering that silver-colored aggregation-polymer is obtained in phosphate buffer solution that sediment is dissolved in again is molten
Liquid；
D. the cushioning liquid of 1-3 milliliters silver aggregation-polymer is taken, the 20-60 microlitres of cushioning liquid containing antibody is added, in 4 DEG C
Lower reaction 2 hours, after centrifugation removes unnecessary unreacted antibody, it is molten to add the 10-30 microlitres of buffering containing bovine serum albumin(BSA)
Liquid, react 1 hour at room temperature, to close the site that silver-colored aggregation-polymer is not adhered to by antibody, centrifugation removes unnecessary not anti-
After the bovine serum albumin(BSA) answered, that is, silver-colored aggregation-polymer SERS immunological probes are obtained, be placed in storing at 4 DEG C stand-by；
(2) preparation of substrate is immunized in Crystal structure
A. the silicon chip of hydrophiling is placed in 1 in the 3- aminopropyl triethoxysilane aqueous solution that concentration is 1.1-3.3 mg/mls
Hour makes amino in its surface modification；The silicon chip for being modified with amino is placed in the gold nano grain aqueous solution that particle diameter is 3-5 nanometers
In 2 hours, make gold nano grain on its adsorption；The silicon chip for being adsorbed with gold nano grain is placed in containing 4- sulfydryl benzene first
After being reacted 15 minutes in the aqueous solution of acid, gold chloride and ascorbic acid, i.e., obtain Crystal structure in silicon chip surface；
B. the 20-60 microlitres of cushioning liquid (concentration is 2-6 mg/mls) containing antibody is added dropwise on Crystal structure in 4 DEG C
Lower reaction 2 hours, after cleaning removes unnecessary unreacted antibody, then that the 10-30 microlitres of buffering containing bovine serum albumin(BSA) is added dropwise is molten
Liquid reacts 1 hour at room temperature, and to close the site do not adhered on Crystal structure by antibody, cleaning removes unnecessary unreacted
Bovine serum albumin(BSA) after obtain Crystal structure substrate be immunized, be placed in storing at 4 DEG C stand-by；
(3) cancer markers detection architecture assembles
Phosphate buffer solution containing determined antigen is added drop-wise into Crystal structure to be immunized in substrate, is placed on quiet at 37 DEG C
Put 2 hours, the immune response between antigen and antibody is fully carried out, cleaning removes unnecessary unreacted determined antigen；Again will
15 microlitres of silver-colored aggregations-polymer SERS immunological probes are added drop-wise to the immune substrate of Crystal structure for being adsorbed with determined antigen
On, reacted 2 hours at 37 DEG C, cleaning removes unnecessary unreacted silver-colored aggregation-polymer SERS immunological probes, that is, obtains base
In the cancer markers detection architecture of silver-colored aggregation-polymer and Crystal structure.
Silver nitrate concentration is 0.2 milligram/milli in the aqueous solution containing silver nitrate and sodium citrate described in step (1) B
Rise and sodium citrate concentration is 0.6 mg/ml；
The concentration of 2- thionaphthols is 10 milligrams/milli in the dimethyl formamide solution of the thionaphthol containing 2- described in step (1) C
Rise；Polystyrene polyacrylic acid in the described dimethyl formamide solution containing polystyrene polyacrylic acid diblock copolymer
The concentration of diblock copolymer is 8 mg/mls；
The concentration of antibody is 2 mg/mls in the cushioning liquid containing antibody described in step (1) D, and cushioning liquid is phosphate
Cushioning liquid；The mass concentration of bovine serum albumin(BSA) is 3% in the described cushioning liquid containing bovine serum albumin(BSA), cushioning liquid
For phosphate buffer solution；
Centrifugal speed is 5000-15000 revs/min in step (1) C and D, and centrifugation time is 5-30 minutes.
4- sulfydryl benzene first in the aqueous solution containing 4- mercaptobenzoic acids, gold chloride and ascorbic acid described in step (2) A
The concentration of acid is 0.85-2.55 mg/mls, and the concentration of gold chloride is 0.07-0.21 mg/mls and the concentration of ascorbic acid
For 0.7-2.1 mg/mls.
The concentration of antibody is 2-6 mg/mls in the cushioning liquid containing antibody described in step (2) B, and cushioning liquid is
Phosphate buffer solution；The mass concentration of bovine serum albumin(BSA) is 3% in the described cushioning liquid containing bovine serum albumin(BSA), is delayed
It is phosphate buffer solution to rush solution.
The application of the above-mentioned cancer markers detection architecture based on silver-colored aggregation-polymer and Crystal structure, by institute
The cancer markers detection architecture based on silver-colored aggregation-polymer and Crystal structure stated carries out light using Raman spectrometer
Spectrometry, according to the quantitative relationship between spectral intensity and antigen concentration, you can calculate phosphate of the acquisition containing determined antigen and delay
Rush the concentration of antigen in solution.
Compared with prior art, the advantage of the invention is that：Present invention firstly discloses based on silver-colored aggregation-polymer
The SERS bases to cancer markers with relatively low detectable limit that substrate is immunized in SERS immunological probes and Crystal structure are immunized
Detection architecture and its application, it forms silver-colored aggregation-polymerization by the high specific immune response between antigen and corresponding antibody
Substrate three-decker is immunized in thing SERS immunological probes/determined antigen/Crystal structure, is imitated using SERS
Should, the chaacteristic fingerprint spectrum of detection immunological probe surface Raman label realizes the detection to cancer markers antigen to be measured, has
Following advantage：
(1) the silver nano-grain number reunited in its silver-colored aggregation-polymer SERS immunological probes in silver-colored aggregation is up to 10
More than, there is the gap taken measurements greatly less than 10 nanometers between each other., can shape in these nano gaps under the exciting of ambient light
Into substantial amounts of Raman hot spot region, compared to the identical size silver nano-grain of individualism, very high strength SERS can be produced
Signal output.And the present invention makes silver nanoparticle by way of using polymer wrapped in the appropriate time after occurring in aggregation
The aggregation of grain terminates, and is a kind of controllable method for preparing silver-colored aggregation, and the number of Argent grain is basic in resulting aggregation
For 10 or so (as shown in Figure 1), make silver-colored aggregation polymeric inner numbers of particles than more uniform.Obtaining, intensity is high
While SERS signal exports, the stability of signal has been obviously improved.In addition, Raman labels molecule is wrapped in polymerization by this method
Within thing, the outer surface of polymer does not have molecule attachment.In the immune response after progress, compared to the table reported before
Face is marked with the gold and silver aggregation of Raman molecular, and antigen-antibody is easily largely adsorbed in the clean outer surface that the present invention obtains
On, the immune detection limit that can also promote to obtain afterwards is greatly lowered.
(2) the nanowires of gold length in the present invention in Crystal structure is bordering on parallel between each other up to 1 microns
It is vertically arranged, because nanowire alignment is close, substantial amounts of nano gap can be formed in array surface.Meanwhile nano wire have compared with
Big surface area/volume ratio, and the lightning rod effect that its tip has.In the presence of external exciting light, nanowires of gold produces
Surface plasma know from experience and propagated and coupled on nano-wire array surface, and produce in nano gap substantial amounts of Raman heat
Point region, it can equally obtain the SERS signal output of higher-strength.After immune response particularly occurs, silver-colored aggregation-polymerization
Thing SERS immunological probes can be attached to Crystal structure and substrate surface is immunized, and plasma coupling can equally occur therebetween
Close, further enhance SERS signal, be advantageous to obtain the test limit extremely low to determined antigen in immune detection.Further, since
The length of nanowires of gold is very unified so that the surface of Crystal structure is very smooth, ensure that after immune response occurs
Uniform coating of the silver-colored aggregation-polymer SERS immunological probes to its surface.This point is the same as silver-colored aggregation polymeric inner particle
The homogeneity of number is together so that the SERS signal obtained has the uniformity of height.Therefore, it is immunized using this programme
Detection has high repeatability, and (Raman signal that 15 retests obtain is as shown in figure 3, its 1064 and 1381cm-1It is strong
Degree standard deviation is only 3.8% and 3.2%) (test limit of prostate specific antigen (PSA) reached with extremely low detectable limit
1 winged gram every milliliter).
(3) technical process of the present invention is simple, and the cycle is short, and cost is low, the clinic easy to spread applied to cancer
Among detection.
Brief description of the drawings
Fig. 1 is the transmission electron microscope of the silver-colored aggregation-polymer SERS immunological probes prepared in the embodiment of the present invention 1
Photo；
Fig. 2 is that the transmission electron microscope photo top view of substrate is immunized in the Crystal structure prepared in the embodiment of the present invention 1
One；
Fig. 3 is that the transmission electron microscope photo top view of substrate is immunized in the Crystal structure prepared in the embodiment of the present invention 1
Two；
Fig. 4 is the silver-colored aggregation-polymer SERS immunological probe and the immune base of Crystal structure prepared in the embodiment of the present invention 1
Bottom carries out the obtained Raman spectrogram of Raman detection after immune response by being carried out with determined antigen to substrate；
Fig. 5 is the cancer markers detection architecture prepared in the embodiment of the present invention 1 containing determined antigen (concentration is 1 milligram every milliliter)
Contrast Raman spectrogram not containing determined antigen；
Fig. 6 is the silver-colored aggregation-polymer SERS immunological probe and the immune base of Crystal structure prepared in the embodiment of the present invention 1
It is right after determined antigen (concentration is 1 milligram every milliliter to 1 winged gram every milliliter) progress immune response with various concentrations that bottom passes through
Substrate carries out the Raman spectrogram that Raman detection obtains；
Fig. 7 is that frequency displacement is 1381cm in the cancer markers detection architecture Raman spectrum prepared in the embodiment of the present invention 1-1Feature
Peak intensity with determined antigen concentration variation diagram.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing embodiment.
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.Unless otherwise specified, embodiment
In the conventional meanses that are well known to those skilled in the art of used technological means, raw materials used is commercial goods.Embodiment
The middle Raman spectrum detector BWS415 used is purchased from Bi Da Imtech of the U.S. (B＆W Tek Inc.).Cancer markers refer to
Directly produced as tumour cell or the material as caused by non-tumor cell through tumor cell induction, following examples are special with prostate
Hapten (PSA), alpha-fetoprotein antigen and CA125 illustrate for example.
Embodiment 1
The preparation method of prostate specific antigen (PSA) detection architecture based on silver-colored aggregation-polymer and Crystal structure,
Comprise the following steps：
1st, the preparation of silver-colored aggregation-polymer SERS immunological probes
1.47 milligrams of sodium citrates and 1.97 milligrams of gold chlorides are dissolved in 20 milliliters of water mixed solution is made, side is violent at room temperature
The sodium borohydride aqueous solution (concentration is 4 mg/mls) that side adds 0.6 milliliter of Fresh is stirred, obtains gold seeds solution；
Take 3 milliliters of gold seeds solution be added to 50 milliliters containing silver nitrate (concentration is 0.2 mg/ml) and sodium citrate it is (dense
Spend for 0.6 mg/ml) the aqueous solution in, be then added dropwise thereto 10 milliliters of aqueous ascorbic acids (concentration be 2 milli
Grams per milliliter), reaction obtains the silver nano-grain aqueous solution in 1 hour；Take 1 milliliter of silver nano-grain aqueous solution centrifugal concentrating micro- for 10
Rise, gradually add dimethyl formamide solution (the wherein 2- of 820 microlitres of dimethylformamides, 5 microlitres of thionaphthols containing 2- thereto
Thionaphthol concentration is 10 mg/mls) and 20 microlitres of water, it is subsequently placed in 60 DEG C of baking ovens after cultivating 3 hours and adds thereto
80 microlitres of dimethyl formamide solutions containing polystyrene polyacrylic acid diblock copolymer (concentration be 8 mg/mls) with
180 microlitres of water, then be placed in 110 DEG C of baking ovens cultivate 2 hours after centrifuge, obtained sediment is silver-colored aggregation-polymer, will
The cushioning liquid of silver-colored aggregation-polymer is obtained in the phosphate buffer solution that sediment is dissolved in again；Take 1 milliliter of silver-colored aggregation-
The cushioning liquid of polymer, adding 20 microlitres of cushioning liquid containing prostate-specific antibody thereto, that 2 are reacted at 4 DEG C is small
When.After centrifugation removes unnecessary unreacted antibody, 10 microlitres of cushioning liquid containing bovine serum albumin(BSA) are added thereto, in room
The lower reaction of temperature 1 hour, to close the site that silver-colored aggregation-polymer is not adhered to by antibody.Centrifugation removes unnecessary unreacted ox
Silver-colored aggregation-polymer SERS immunological probes are obtained after seralbumin, are placed in storing at 4 DEG C stand-by.Wherein contain antibody
Cushioning liquid in the concentration of antibody be 2 mg/mls, cushioning liquid is phosphate buffer solution；Containing bovine serum albumin(BSA)
The mass concentration of bovine serum albumin(BSA) is 3% in cushioning liquid, and cushioning liquid is phosphate buffer solution；Centrifugal speed is 5000
Rev/min, centrifugation time is 30 minutes.
Fig. 1 shows the transmission electron microscope of the silver-colored aggregation-polymer SERS immunological probes prepared in the present embodiment
Photo.From figure 1 it appears that being core shell structure in prepared immunological probe, center is the aggregation of a large amount of silver nano-grains
Body, outer layer are polymeric shell layer.
2nd, the preparation of substrate is immunized in Crystal structure
Hydrophilicity-imparting treatment is done to the silicon chip surface that size is 1 square centimeter by plasma clean.The silicon chip of hydrophiling is put
1 hour in the APTES aqueous solution (concentration is 1.1 mg/mls), make amino in its surface modification.The silicon of amino will be modified with
Piece is placed in the gold nano grain aqueous solution that particle diameter is 3-5 nanometers 2 hours, makes gold nano grain on its adsorption.Will absorption
The silicon chip for having gold nano grain is placed in that (concentration is containing 4- mercaptobenzoic acids (concentration is 0.85 mg/ml), gold chloride
0.07 mg/ml) and the aqueous solution of ascorbic acid (concentration is 0.7 mg/ml) in react after 15 minutes i.e. in silicon chip
Surface obtains Crystal structure.The cushioning liquid of 20 microlitres of antibody containing prostate-specific is added dropwise on to Crystal structure in 4
Reacted 2 hours at DEG C.After cleaning removes unnecessary unreacted antibody, to 10 microlitres of bufferings containing bovine serum albumin(BSA) of dropwise addition thereon
Solution reacts 1 hour at room temperature, to close the site do not adhered on Crystal structure by antibody.Cleaning removes unnecessary not anti-
Crystal structure is obtained after the bovine serum albumin(BSA) answered substrate is immunized, be placed in storing at 4 DEG C stand-by.Wherein containing antibody
The concentration of antibody is 2 mg/mls in cushioning liquid, and cushioning liquid is phosphate buffer solution；It is slow containing bovine serum albumin(BSA)
The mass concentration for rushing bovine serum albumin(BSA) in solution is 3%, and cushioning liquid is phosphate buffer solution.
Fig. 2 shows that the transmission electron microscope photo top view of substrate is immunized in the Crystal structure prepared in the present embodiment
Figure, figure it is seen that a large amount of nano gaps are contained on prepared Crystal structure surface.
Fig. 3 shows that the transmission electron microscope photo top view of substrate is immunized in the Crystal structure prepared in the present embodiment
Figure, from figure 3, it can be seen that prepared Crystal structure is approximately perpendicular to silicon chip, arrangement is fine and close.
3rd, cancer markers detection architecture assembles
Phosphate buffer solution containing prostate specific antigen to be measured is added drop-wise into Crystal structure to be immunized in substrate, put
2 hours are stood at 37 DEG C, the immune response between antigen and antibody is fully carried out, cleaning removes unnecessary unreacted to be measured
Antigen；15 microlitres of silver-colored aggregations-polymer SERS immunological probes are added drop-wise to the Crystal structure for being adsorbed with determined antigen again
In immune substrate, to be reacted 2 hours at 37 DEG C, cleaning removes unnecessary unreacted silver-colored aggregation-polymer SERS immunological probes,
Obtain the cancer markers detection architecture based on silver-colored aggregation-polymer and Crystal structure.
4th, spectrum survey is carried out to the cancer markers detection architecture obtained after above-mentioned steps are handled using Raman spectrometer
The detectable determined antigen of amount.
Fig. 4 is to be exempted from using the silver-colored aggregation-polymer SERS immunological probes and Crystal structure that are prepared in the present embodiment
Epidemic disease substrate is by with determined antigen (concentration is 10 micrograms per millilitres) obtain substrate progress Raman detection after immune response
The Raman spectrogram arrived, the Raman signal that 15 retests obtain can be calculated from Fig. 4, its 1064 and 1381cm-1
Intensity standard deviation be only 3.8% and 3.2%.
Fig. 5 is to be exempted from using the silver-colored aggregation-polymer SERS immunological probes and Crystal structure that are prepared in the present embodiment
Epidemic disease substrate is by with determined antigen (concentration is 1 milligram every milliliter) obtain substrate progress Raman detection after immune response
Raman spectrogram.From fig. 5, it can be seen that when containing determined antigen, the stronger of mark molecule 2- thionaphthols can be detected
Raman signatures are composed, it is in 1382cm-1The Raman signal intensity at place reaches 19957.The detection pole of prostate specific antigen (PSA)
Limit can reach 1 winged gram every milliliter.And when not containing determined antigen in solution to be measured, because silver-colored aggregation-polymer SERS exempts from
Epidemic disease probe and Crystal structure are immunized substrate and can not be combined by specific immune response, and only occur a small amount of non-specific
Property absorption, therefore, only measure very weak Raman spectrum.
Fig. 6 is to be exempted from using the silver-colored aggregation-polymer SERS immunological probes and Crystal structure that are prepared in the present embodiment
Epidemic disease substrate by determined antigen with various concentrations (concentration is 1 milligram every milliliter to 1 and flies gram every milliliter) carry out immune response it
The Raman spectrogram that Raman detection obtains is carried out to substrate afterwards.From fig. 6, it can be seen that work as with the reduction of determined antigen concentration,
The Raman signatures spectral intensity of mark molecule 2- thionaphthols gradually reduces, until the concentration of determined antigen is reduced to 1 winged gram every milliliter
When, the raman characteristic peak of mark molecule is relative to background signal still it is obvious that this concentration is this programme to determined antigen
Detectable limit.
Fig. 7 is that frequency displacement is 1381cm in Raman spectrum-1Feature peak intensity with the variation diagram of determined antigen concentration, pass through fitting
It can be seen that when the concentration of determined antigen is from when changing to 1 milligram every milliliter for 1 winged gram every milliliter, Raman signatures peak intensity is with concentration
Linear change.Fitting result shows that this variation tendency meets this linear equation of Y=2245.42+32865.44*LogX,
Standard deviation is 0.985, and wherein X is the concentration of antigen, and Y is Raman signatures peak intensity.
Embodiment 2
The preparation method of alpha-fetoprotein antigen detection architecture based on silver-colored aggregation-polymer and Crystal structure, including with
Lower step：
1st, the preparation of silver-colored aggregation-polymer SERS immunological probes：2.94 milligrams of sodium citrates and 3.94 milligrams of gold chlorides are molten
Mixed solution is made in 40 milliliters of water, the sodium borohydride for adding 1.2 milliliters of Fresh when being stirred vigorously at room temperature is water-soluble
Liquid (concentration is 4 mg/mls), obtains gold seeds solution；Take 6 milliliters of gold seeds solution to be added to 100 milliliters and contain silver nitrate
In (concentration is 0.2 mg/ml) and the aqueous solution of sodium citrate (concentration is 0.6 mg/ml), add dropwise thereto afterwards
Enter 20 milliliters of aqueous ascorbic acids (concentration is 2 mg/mls), reaction obtains the silver nano-grain aqueous solution in 1 hour；
It is 20 microlitres to take 2 milliliters of silver nano-grain aqueous solution centrifugal concentratings, gradually adds 1640 microlitres of dimethyl formyls thereto
Amine, the dimethyl formamide solution and 40 microlitres of water of 10 microlitres of thionaphthols containing 2- (concentration is 10 mg/mls), is subsequently placed in
160 microlitres, which are added, after being cultivated in 60 DEG C of baking ovens 3 hours thereto contains polystyrene polyacrylic acid diblock copolymer (concentration
For 8 mg/mls) dimethyl formamide solution and 360 microlitres of water, be subsequently placed in after being cultivated 2 hours in 110 DEG C of baking ovens and centrifuge
In the phosphate buffer solution that sediment (i.e. silver-colored aggregation-polymer) is dissolved in again；Take 2 milliliters of silver-colored aggregation-polymer
Cushioning liquid, it is anti-at 4 DEG C that 40 microlitres of cushioning liquid containing alpha-fetoprotein antibody (concentration is 2 mg/mls) are added thereto
Answer 2 hours, after centrifugation removes unnecessary unreacted antibody, add the cushioning liquid (matter of 20 microlitres of bovine serum albumin(BSA)s thereto
Percentage is measured 3%), to react 1 hour at room temperature, to close the site that silver-colored aggregation-polymer is not adhered to by antibody, centrifugation
Silver-colored aggregation-polymer SERS immunological probes are obtained after removing unnecessary unreacted bovine serum albumin(BSA), are placed in storing up at 4 DEG C
Deposit stand-by.Wherein centrifugal speed is 10000 revs/min, and centrifugation time is 20 minutes.
2nd, the preparation of substrate is immunized in Crystal structure
Hydrophilicity-imparting treatment is done to the silicon chip surface that size is 1 square centimeter by plasma clean.The silicon chip of hydrophiling is put
1 hour in the APTES aqueous solution (concentration is 1.1-3.3 mg/mls), make amino in its surface modification.Amino will be modified with
Silicon chip be placed in size be 3-5 nanometers the gold nano grain aqueous solution in 2 hours, make gold nano grain on its adsorption.Will
The silicon chip for being adsorbed with gold nano grain is placed in that (concentration is containing 4- mercaptobenzoic acids (concentration is 1.7 mg/mls), gold chloride
0.14 mg/ml) and the aqueous solution of ascorbic acid (concentration is 0.14 mg/ml) in react after 15 minutes i.e. in silicon chip
Surface obtains Crystal structure, to Crystal structure on be added dropwise 40 microlitres containing alpha-fetoprotein antibody (concentration be 4 milligrams/milli
Rise) cushioning liquid react 2 hours at 4 DEG C, after cleaning removes unnecessary unreacted antibody, to 20 microlitres of ox bloods of dropwise addition thereon
The cushioning liquid (mass percent 3%) of pure albumen reacts 1 hour at room temperature, with close on Crystal structure not by
The site of antibody attachment, cleaning obtain the immune substrate of Crystal structure after removing unnecessary unreacted bovine serum albumin(BSA),
It is placed in storing at 4 DEG C stand-by.
3rd, cancer markers detection architecture assembles
Phosphate buffer solution containing alpha-fetoprotein antigen to be measured is added drop-wise into Crystal structure to be immunized in substrate, is placed on
2 hours are stood at 37 DEG C, the immune response between antigen and antibody is fully carried out, cleaning removes unnecessary unreacted to be measured anti-
It is former；15 microlitres of silver-colored aggregations-polymer SERS immunological probes are added drop-wise to again and is adsorbed with the Crystal structure of determined antigen and exempts from
In epidemic disease substrate, reacted 2 hours at 37 DEG C, cleaning removes unnecessary unreacted silver-colored aggregation-polymer SERS immunological probes, i.e.,
Obtain the cancer markers detection architecture based on silver-colored aggregation-polymer and Crystal structure.
4th, spectrum survey is carried out to the cancer markers detection architecture obtained after above-mentioned steps are handled using Raman spectrometer
The detectable determined antigen of amount.
Substrate is immunized using the silver-colored aggregation-polymer SERS immunological probes and Crystal structure prepared in the present embodiment
Pass through the Raman obtained afterwards to substrate progress Raman detection with determined antigen (concentration is 1 milligram every milliliter) progress immune response
Spectrogram can be seen that when containing determined antigen, and can detect mark molecule 2- thionaphthols relatively hales graceful characteristic spectrum, its
In 1382cm-1The Raman signal intensity at place reaches 17363.The detectable limit of alpha-fetoprotein antigen can reach 2 winged grams every milliliter.
And when not containing determined antigen in solution to be measured, due to silver-colored aggregation-polymer SERS immunological probes and Crystal structure
Immune substrate can not be combined by specific immune response, and a small amount of non-specific adsorption only occurs, and therefore, only be measured very
Weak Raman spectrum.
Embodiment 3
The preparation method to CA125 detection architecture based on silver-colored aggregation-polymer and Crystal structure, including
Following steps：
1st, the preparation of silver-colored aggregation-polymer SERS immunological probes
4.41 milligrams of sodium citrates and 5.91 milligrams of gold chlorides are dissolved in 60 milliliters of water mixed solution is made, side is violent at room temperature
The sodium borohydride aqueous solution (concentration is 4 mg/mls) that side adds 1.8 milliliters of Fresh is stirred, obtains gold seeds solution；
Take 9 milliliters of gold seeds solution be added to 150 milliliters containing silver nitrate (concentration is 0.2 mg/ml) and sodium citrate it is (dense
Spend for 0.6 mg/ml) the aqueous solution in, be added dropwise thereto afterwards 30 milliliters of aqueous ascorbic acids (concentration be 2 milli
Grams per milliliter), reaction obtains the silver nano-grain aqueous solution in 1 hour；Take 3 milliliters of silver nano-grain aqueous solution centrifugal concentratings micro- for 30
Rise, thereto gradually add 2460 microlitres of dimethylformamides, the two of 15 microlitres of thionaphthols containing 2- (concentration is 10 mg/mls)
NMF solution and 60 microlitres of water, it is poly- that thereto addition 240 microlitre is subsequently placed in 60 DEG C of baking ovens after cultivating 3 hours
The dimethyl formamide solution and 540 microlitres of water of styrene polyacrylic acid diblock copolymer (concentration is 8 mg/mls), then
It is molten that the phosphate-buffered that sediment (i.e. silver-colored aggregation-polymer) is dissolved in by centrifugation again after cultivating 2 hours is placed in 110 DEG C of baking ovens
In liquid；The cushioning liquid of 3 milliliters of silver-colored aggregation-polymer is taken, adding 60 microlitres thereto, (concentration is 2 millis containing carbohydrate antibodies
Grams per milliliter) cushioning liquid reacted 2 hours at 4 DEG C.After centrifugation removes unnecessary unreacted antibody, it is micro- that 30 are added thereto
The cushioning liquid (mass percent 3%) of bovine serum albumin(BSA) is risen, is reacted 1 hour at room temperature, to close silver-colored aggregation-poly-
The site that compound is not adhered to by antibody.Centrifugation obtains silver-colored aggregation-polymerization after removing unnecessary unreacted bovine serum albumin(BSA)
Thing SERS immunological probes, it is placed in storing at 4 DEG C stand-by.Wherein centrifugal speed is 15000 revs/min, and centrifugation time is 5 points
Clock.
2nd, the preparation of substrate is immunized in Crystal structure
Hydrophilicity-imparting treatment is done to the silicon chip surface that size is 1 square centimeter by plasma clean.The silicon chip of hydrophiling is put
1 hour in the APTES aqueous solution (concentration is 3.3 mg/mls), make amino in its surface modification.The silicon of amino will be modified with
Piece is placed in the gold nano grain aqueous solution that size is 3-5 nanometers 2 hours, makes gold nano grain on its adsorption.Will absorption
The silicon chip for having gold nano grain is placed in containing 4- mercaptobenzoic acids (concentration is 2.55 mg/mls), and (concentration is gold chloride
0.21 mg/ml) and the aqueous solution of ascorbic acid (concentration is 2.1 mg/mls) in react after 15 minutes i.e. in silicon chip
Surface obtains Crystal structure.60 microlitres of (concentration are 6 mg/mls) containing carbohydrate antibodies are added dropwise on to Crystal structure
Cushioning liquid reacted 2 hours at 4 DEG C.After cleaning removes unnecessary unreacted antibody, to 30 microlitres of cow's serums of dropwise addition thereon
The cushioning liquid (mass percent 3%) of albumin reacts 1 hour at room temperature, is not resisted on Crystal structure with closing
The site of body attachment.Cleaning obtains the immune substrate of Crystal structure after removing unnecessary unreacted bovine serum albumin(BSA), and
Be placed at 4 DEG C store it is stand-by.
3rd, cancer markers detection architecture assembles
Phosphate buffer solution containing sugar antigen to be measured is added drop-wise into Crystal structure to be immunized in substrate, is placed on 37 DEG C
It is lower to stand 2 hours, the immune response between antigen and antibody is fully carried out, cleaning removes unnecessary unreacted determined antigen；
15 microlitres of silver-colored aggregations-polymer SERS immunological probes are added drop-wise to again and is adsorbed with the Crystal structure of determined antigen and is immunized
In substrate, reacted 2 hours at 37 DEG C, cleaning removes unnecessary unreacted silver-colored aggregation-polymer SERS immunological probes, produces
To the cancer markers detection architecture based on silver-colored aggregation-polymer and Crystal structure.
4th, the immune substrate progress spectral measurement obtained after above-mentioned steps are handled can detect using Raman spectrometer and treats
Survey antigen.
Substrate is immunized using the silver-colored aggregation-polymer SERS immunological probes and Crystal structure prepared in the present embodiment
Pass through the Raman obtained afterwards to substrate progress Raman detection with determined antigen (concentration is 1 milligram every milliliter) progress immune response
Spectrogram can be seen that when containing determined antigen, and can detect mark molecule 2- thionaphthols relatively hales graceful characteristic spectrum, its
In 1382cm-1The Raman signal intensity at place reaches 15221.The detectable limit of CA125 reaches 2 winged grams every milliliter.And
When not containing determined antigen in solution to be measured, because silver-colored aggregation-polymer SERS immunological probes and Crystal structure are exempted from
Epidemic disease substrate can not be combined by specific immune response, and a small amount of non-specific adsorption only occurs, and therefore, only be measured very weak
Raman spectrum.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (5)
1. a kind of preparation method of the cancer markers detection architecture based on silver-colored aggregation-polymer and Crystal structure, its
It is characterised by comprising the following steps：
（1）The preparation of silver-colored aggregation-polymer SERS immunological probes
A. by 1.47-4.41 milligrams sodium citrate and 1.97-5.91 milligram gold chlorides be dissolved in 20-60 milliliter water be made mixing it is molten
Liquid, the concentration for adding 0.6-1.8 milliliter Fresh when being stirred vigorously at room temperature is the sodium borohydride water of 4 mg/mls
Solution, obtain gold seeds solution；
B. 3-9 milliliter gold seeds solution is taken to be added in the aqueous solution of the 50-150 milliliters containing silver nitrate and sodium citrate, afterwards
The aqueous ascorbic acid that 10-30 milliliters concentration is 2 mg/mls is added dropwise thereto, reaction obtains silver nanoparticle in 1 hour
The grain aqueous solution；
C. it is 10-30 microlitres to take 1-3 milliliter silver nano-grain aqueous solution centrifugal concentrating, gradually adds 820-2460 microlitres thereto
Dimethylformamide, the dimethyl formamide solution and 20-60 microlitres of water of the 5-15 microlitres of thionaphthol containing 2-, it is subsequently placed in 60 DEG C of bakings
After being cultivated 3 hours in case, the 80-240 microlitres of diformazan containing polystyrene polyacrylic acid diblock copolymer is added thereto
Base formamide solution and 180-540 microlitres of water, it is subsequently placed in after being cultivated 2 hours in 110 DEG C of baking ovens and centrifuges, obtained sediment is i.e.
It is for silver-colored aggregation-polymer, the buffering that silver-colored aggregation-polymer is obtained in phosphate buffer solution that sediment is dissolved in again is molten
Liquid；
D. the cushioning liquid of 1-3 milliliters silver aggregation-polymer is taken, the 20-60 microlitres of cushioning liquid containing antibody is added, in 4 DEG C
Lower reaction 2 hours, after centrifugation removes unnecessary unreacted antibody, it is molten to add the 10-30 microlitres of buffering containing bovine serum albumin(BSA)
Liquid, react 1 hour at room temperature, to close the site that silver-colored aggregation-polymer is not adhered to by antibody, centrifugation removes unnecessary not anti-
After the bovine serum albumin(BSA) answered, that is, silver-colored aggregation-polymer SERS immunological probes are obtained, be placed in storing at 4 DEG C stand-by；
（2）The preparation of substrate is immunized in Crystal structure
A. the silicon chip of hydrophiling is placed in the 3- aminopropyl triethoxysilane aqueous solution that concentration is 1.1-3.3 mg/mls
Make amino in its surface modification within 1 hour；The silicon chip for being modified with amino is placed in the gold nano grain aqueous solution that particle diameter is 3-5 nanometers
In 2 hours, make gold nano grain on its adsorption；The silicon chip for being adsorbed with gold nano grain is placed in containing 4- sulfydryl benzene first
After being reacted 15 minutes in the aqueous solution of acid, gold chloride and ascorbic acid, i.e., obtain Crystal structure in silicon chip surface；
B. the 20-60 microlitres of cushioning liquid containing antibody is added dropwise on Crystal structure（Concentration is 2-6 mg/mls）In 4 DEG C
Lower reaction 2 hours, after cleaning removes unnecessary unreacted antibody, then that the 10-30 microlitres of buffering containing bovine serum albumin(BSA) is added dropwise is molten
Liquid reacts 1 hour at room temperature, and to close the site do not adhered on Crystal structure by antibody, cleaning removes unnecessary unreacted
Bovine serum albumin(BSA) after obtain Crystal structure substrate be immunized, be placed in storing at 4 DEG C stand-by；
（3）Cancer markers detection architecture assembles
Phosphate buffer solution containing determined antigen is added drop-wise into Crystal structure to be immunized in substrate, is placed on quiet at 37 DEG C
Put 2 hours, the immune response between antigen and antibody is fully carried out, cleaning removes unnecessary unreacted determined antigen；Again will
15 microlitres of silver-colored aggregations-polymer SERS immunological probes are added drop-wise to the immune substrate of Crystal structure for being adsorbed with determined antigen
On, reacted 2 hours at 37 DEG C, cleaning removes unnecessary unreacted silver-colored aggregation-polymer SERS immunological probes, that is, obtains base
In the cancer markers detection architecture of silver-colored aggregation-polymer and Crystal structure.
2. according to claim 1 detect body based on the cancer markers of silver-colored aggregation-polymer and Crystal structure
The preparation method of system, it is characterised in that：
Step（1）In the aqueous solution containing silver nitrate and sodium citrate described in B silver nitrate concentration be 0.2 mg/ml with
And sodium citrate concentration is 0.6 mg/ml；
Step（1）The concentration of 2- thionaphthols is 10 milligrams/milli in the dimethyl formamide solution of the thionaphthol containing 2- described in C
Rise；Polystyrene polyacrylic acid in the described dimethyl formamide solution containing polystyrene polyacrylic acid diblock copolymer
The concentration of diblock copolymer is 8 mg/mls；
Step（1）The concentration of antibody is 2 mg/mls in the cushioning liquid containing antibody described in D, and cushioning liquid is phosphate
Cushioning liquid；The mass concentration of bovine serum albumin(BSA) is 3% in the described cushioning liquid containing bovine serum albumin(BSA), cushioning liquid
For phosphate buffer solution.
3. according to claim 1 detect body based on the cancer markers of silver-colored aggregation-polymer and Crystal structure
The preparation method of system, it is characterised in that：Step（1）Centrifugal speed is 5000-15000 revs/min in C and D, centrifugation time 5-
30 minutes.
4. according to claim 1 detect body based on the cancer markers of silver-colored aggregation-polymer and Crystal structure
The preparation method of system, it is characterised in that：Step（2）The water containing 4- mercaptobenzoic acids, gold chloride and ascorbic acid described in A
The concentration of 4- mercaptobenzoic acids is 0.85-2.55 mg/mls in solution, and the concentration of gold chloride is 0.07-0.21 mg/mls
Concentration with ascorbic acid is 0.7-2.1 mg/mls；Step（2）Antibody is dense in the cushioning liquid containing antibody described in B
It is phosphate buffer solution to spend for 2-6 mg/mls, cushioning liquid；Ox in the described cushioning liquid containing bovine serum albumin(BSA)
Sero-abluminous mass concentration is 3%, and cushioning liquid is phosphate buffer solution.
5. a kind of application of the described cancer markers detection architecture based on silver-colored aggregation-polymer and Crystal structure,
It is characterized in that：Utilized described based on the cancer markers detection architecture of silver-colored aggregation-polymer and Crystal structure
Raman spectrometer carries out spectral measurement, according to the quantitative relationship between spectral intensity and antigen concentration, you can calculates to obtain to contain and treats
Survey the concentration of antigen in the phosphate buffer solution of antigen.
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Effective date of registration: 20221223 Address after: Floor 3, Building 3, Pansheng Biological Industrial Park, Gangyuan 8th Road, Jinan Area, China (Shandong) Pilot Free Trade Zone, 250000, Jinan, Shandong Patentee after: Saier Medical Technology (Shandong) Co.,Ltd. Address before: Room 2202, 22 / F, Wantong building, No. 3002, Sungang East Road, Sungang street, Luohu District, Shenzhen City, Guangdong Province Patentee before: Shenzhen dragon totem technology achievement transformation Co.,Ltd. Effective date of registration: 20221223 Address after: Room 2202, 22 / F, Wantong building, No. 3002, Sungang East Road, Sungang street, Luohu District, Shenzhen City, Guangdong Province Patentee after: Shenzhen dragon totem technology achievement transformation Co.,Ltd. Address before: 315211, Fenghua Road, Jiangbei District, Zhejiang, Ningbo 818 Patentee before: Ningbo University |