CN107640443A

CN107640443A - One kind detection sample stored frozen device

Info

Publication number: CN107640443A
Application number: CN201710918321.3A
Authority: CN
Inventors: 张燕; 刘辉; 廖文彬; 刘贵州; 朱小钿; 熊波; 吴胜泽; 梁祖培; 陈志波; 罗永潮; 朱建恩; 苏焕斌
Original assignee: Guangdong Testing Institute of Product Quality Supervision
Current assignee: Guangdong Testing Institute of Product Quality Supervision
Priority date: 2017-09-30
Filing date: 2017-09-30
Publication date: 2018-01-30

Abstract

The present invention provides a kind of detection sample stored frozen device, including casing, freeze proof flexible pipe, tells that casing includes outer case and internal box, the internal box is inside the outer case, characterized in that, the outer case is additionally provided with liquid nitrogen plan, the liquid nitrogen plan is located at the side of the internal box；The tank wall of the internal box is provided with heat-insulation layer, waterproof layer, the waterproof layer is symmetrically set in the heat-insulation layer both sides, the interior case lid is sequentially provided with metal level, heat-insulation layer, waterproof layer, the first hollow sandwich, the tank floor is additionally provided with the second hollow sandwich, first hollow sandwich is arranged with spray orifice, and second hollow sandwich is arranged with spray orifice.Shock-absorbing capacity of the present invention is good, safety and stability, is convenient for carrying, is easy to use, is adapted to the cold storage container as collection sample in sampling process out of doors, sample can be preserved in the case where being not connected to power supply 17 days.

Description

One kind detection sample stored frozen device

Technical field

The present invention relates to food inspection to sample storage device auxiliary equipment, and in particular to one kind detection sample stored frozen dress Put.

Background technology

" bread is the staff of life ", diet are the main ways that human body draws the nutrition depended on for existence and energy from external environment Footpath, it is basis and the performance of vital movement；" eat with An Weixian ", the safety of food is not only related to the existence of people and body is good for Health, and it is related to the quality and the ups and downs of nationality, a national reputation is also directly influenced in international trade contacts.China There are 23 provinces, wherein 14 coastal, east and southern mainland coastline are up to the waters of km more than 1.8 ten thousand, inland sea and Bian Hai Area ten thousand sq-km about more than 470, proportion of the aquatic products in food is even more constantly growth in recent years, and the safety of aquatic food is also It is taken seriously.

Be contaminated in the growth waters of aquatic products or breeding process in the illegal additive prohibited using country or Using the additive of excess, the risk that can all directly contribute aquatic food steeply rises.It then becomes necessary to strengthen aquatic food Sampling supervision, irregularly carries out the work of aquatic products food security sampling Detection, progressively expands aquatic products food sampling inspection covering Face, the selective examination batch to excessive risk aquatic products food is increased, constantly lift the disposal energy of problem aquatic products food after sampling Detection Power.The accountability of serious aquatic products food safety Regulation is called to account mechanism, constantly strengthens supervise and examine dynamics, unimpeded aquatic products food security is lifted Report channel, it is ensured that aquatic products food security zero defects.

Out of doors in sampling process, some specific index parameters need can under conditions of aquatic products freshness is kept Obtain accurate data, and from the time interval sampled into test in laboratory because the geographical position of sampling position is different and nothing Method ensures.Therefore, the characteristics of change perishable based on aquatic food, in general refrigeration means can not reach fresh-keeping requirement.Therefore, Typically the refrigerating chamber in sampling location can will be put into by quick-frozen sample, then pass through refrigerating box by the way of liquid nitrogen flash freezer Transport detection base laboratory back, it is ensured that the freshness of testing sample.Liquid nitrogen container is divided into nitrogen storage tank in the prior art, liquid nitrogen is transported Defeated tank, hold-up tank are mainly used in the standing storage of indoor liquid nitrogen, should not make long distance transportation use in the operating condition；Liquid nitrogen is transported Defeated tank has made special aseismatic design to meet the condition of transport, but volume is big and heavy, inconvenient to carry.To adopt Refrigerated source by the use of liquid nitrogen as sampling refrigerating box, then higher requirement is proposed to the antidetonation buffering stability of refrigerating box.

Therefore, it is badly in need of providing a kind of detection sample stored frozen device, using liquid nitrogen as refrigerated source, is carried for detected sample For quick-freezing fresh-keeping.

The content of the invention

In view of this, a kind of detection sample stored frozen device provided by the invention, using the structure of multi-buffer, buffering Functional, used liquid nitrogen plan outer tank body can have certain slip with respect to inner tank body, coordinate the flexible sealing of the present invention The a certain degree of stretching of part, so as to change the volume of tank body, to reduce the tank body caused by rocking during transport or use Pressure caused by internal pressure rise, the security of the present invention is improved, extends product useful life.The present invention can be to the sample of collection Product rapidly and uniformly freeze, and extend the freshness pot-life of sample, safety and stability, are convenient for carrying, are easy to use, are adapted to out of doors Cold storage container in sampling process as collection sample.

The technical scheme is that：One kind detection sample stored frozen device, including casing, freeze proof flexible pipe, tell case Body includes outer case and internal box, and the internal box is inside the outer case, it is characterised in that the outer case is additionally provided with Liquid nitrogen plan, the liquid nitrogen plan are located at the side of the internal box；

The outer case is provided with outer case lid, and symmetrically staggeredly elastomer and magnetic block are provided with the inside of the outer case lid；

The internal box is provided with interior case lid, and the elastomer on the outside of the interior case lid with offseting, the magnetic block and the interior case Outer side of lid connects；

The tank wall of the internal box is provided with heat-insulation layer, waterproof layer, and the waterproof layer is symmetrically set in the heat-insulation layer both sides, described interior Case lid is sequentially provided with metal level, heat-insulation layer, waterproof layer, the first hollow sandwich, and the tank floor is additionally provided with the second hollow sandwich, The liquid nitrogen plan is connected with first hollow sandwich, the second hollow sandwich respectively by freeze proof flexible pipe；Interior case in the present invention The structure of body can provide an effective thermal environment for collection sample, while have preferable water resistance.The insulation Layer can realize that the waterproof layer is in the prior art can be by of the prior art resistance to low by insulation material of the prior art Warm waterproof material is realized.

In the present invention, it can be realized using elastomer and magnetic block by any prior art, pass through elastomer and magnetic block Outer case and internal box are fitted close in upper end, after outer case lid is closed, the elastomer offsets with the interior case lid surface, There can be certain cushioning effect to the stress of tank surface；The magnetic block can be adsorbed with the metal level of internal box, in restriction The position of casing, avoid internal box shift influence outer case inside resistance to overturning.

The freeze proof flexible pipe and the first hollow sandwich junction are provided with the first plenum member, the freeze proof flexible pipe with it is described Second hollow sandwich junction is provided with the second plenum member；

First hollow sandwich is arranged with spray orifice, and second hollow sandwich is arranged with spray orifice；

The outer case bottom includes damp-proof layer, heat-insulated cushion, the first magnet layer, the second magnet layer, elastomer layer, wear-resisting Layer, the first magnet layer are located at the side of the elastomer layer, and the second magnet layer is another located at the elastomer layer Side, the heat-insulated cushion are respectively arranged on the first magnet layer, the side of the second magnet layer, the damp-proof layer located at it is described every The side of Heat buffered layer, the wearing layer is located at the side of the heat-insulated cushion and positioned at the lower end of the outer case bottom. In the present invention, the elastomer layer is ethylene octane copolymer layer of the prior art, the ethylene octane copolymer （Polyolefin Elastomer, abbreviation POE）With excellent elastic performance, compared with the rubber of traditional chemical crosslinking, POE The required cost of acquisition elasticity is lower, quality is lighter, energy consumption is lower, to more environment-friendly, is widely used to automobile zero at present The field such as part, electric wire, machine tool, household articles, toy, amusement and sporting goods, sole, seal, PUR. The thermal insulation layer is SiO₂Aerogel heat-proof layer, the SiO₂Aerogel heat-proof layer can be realized by any prior art.It is described Damp-proof layer can be realized by barrier material of the prior art.By the setting of the thermal insulation layer, elastomer layer of outer case, match somebody with somebody simultaneously The positioning of pad of fitting is fixed, and can have preferable buffering and damping effect；Also there is excellent heat-holding dampproof performance.

Particularly, the first magnet layer and the second magnet layer are Permanent-magnet layer, the first magnet layer and the second magnetic The magnetic of body layer is repelled each other.By outside pressure after, the first magnet layer is pressed down against elastomer layer, by the first magnet layer with The magneticaction to repel each other of second magnet layer, further elastomer-assisted layer resilience buffering, optimizes buffering effect.

Particularly, the outer case is provided with heat insulating coat provided with heat-insulated outside, and the heat insulating coat is SiO₂Aeroge every Hot coating, the SiO₂Aerogel heat-proof coating can be realized by any prior art.The heat of outside can effectively be completely cut off, kept away Exempt from that heat transfer occurs with box house, effectively extend the insulation time limit.

The Fluid pressure of the first plenum member outlet is the 0.5-0.75 of the Fluid pressure of the second plenum member outlet Times.It is less than from the Fluid pressure of the spray orifice of the first hollow sandwich the fluid pressure from the spray orifice of the second hollow sandwich i.e. in the present invention Power, so frozen liquid can be caused fully to be contacted with sample surfaces, make temperature field in internal box more uniform, avoid sample only Only the situation of freezing partly occurs so that freezing is more uniform, and refrigerating effect is more preferable.

Particularly, freeze proof flexible pipe of the invention can be realized by this area any prior art, and can pass through existing skill The freeze proof flexible pipe is provided in the heat-insulation layer of side tank wall of the internal box by art, then respectively with the described first hollow folder Layer, the connection of the second hollow sandwich.

In the present invention, after opening liquid nitrogen plan, liquid nitrogen fluid respectively enters the first hollow sandwich, second by freeze proof flexible pipe Hollow sandwich, then frozen liquid is sprayed from top, from bottom by spray orifice respectively, it is ensured that the freezing uniformity of sample is gathered, is had Effect improves the pot-life of testing sample.

The horizontal cross-section of the spray orifice is isosceles trapezoid.The shape of the spray orifice can effectively improve the air pressure of bottom flow, So that freezing is more uniform.

Particularly, the outer case lid realizes that folding connects by hinge arrangement of the prior art, the outer case lid with it is outer Casing junction is provided with the sealing strip of prior art, strengthens sealing property；The interior case lid passes through hinge knot of the prior art Structure realizes that folding connects, and the interior case lid is provided with the sealing strip of prior art with internal box junction, strengthens sealing property.

The liquid nitrogen plan includes tank body, and the tank body is provided with exhaust outlet, and the freeze proof flexible pipe is connected with exhaust outlet, institute State exhaust outlet and be provided with valve member, the valve member includes the components such as atmospheric valve, pressure charging valve, can be any existing by this area Technology is realized.The tank body lower end is provided with base, and the tank body includes outer tank body, inner tank body, and the inner tank body is located at outer tank body It is internal, it is characterised in that the outer tank body lower ending opening, the inner tank body upper end open, the tank body is interior to be provided with flexible sealing Part, one end of the flexible seals and the upper end of inner tank body connect, the other end and the outer tank body inner side of the flexible seals Upper end connection；

The outer tank body lower end is provided with first connecting portion, and the base upper end is provided with second connecting portion, and the first connecting portion is Permanent magnet connecting portion, the second connecting portion are permanent magnet connecting portion, and the first connecting portion and second connecting portion magnetic are attracting, The first connecting portion is connected with second connecting portion；

The internal face of the outer tank body is arranged with neck, and the neck is provided with magnetic absorption layer, the outer wall of the inner tank body Face is arranged with metal barb, and the neck is connected with metal barb；

Particularly, the flexible seals are coaxially disposed with the tank body.

Further, the flexible seals are the hollow tube-shape seal of side indent.

In the present invention, reaching certain pressure condition, outer tank body there can be certain slip with respect to inner tank body, coordinate this hair The a certain degree of stretching of bright flexible seals, so as to change the volume of tank body, to reduce because of the rolling during transport or use Pressure caused by tank body internal pressure rise caused by dynamic, extends product useful life.In extreme circumstances, when tank body internal pressure is too high When, under pressure, outer tank body and inner tank body can strut automatically, avoid internal pressure too high and produce potential safety hazard.Flexible sealing Part can connect the inwall of inner tank body upper end and inner tank body, realize the air-tightness of the two seam crossing.Flexible seals can be selected any A kind of flexible encapsulant is made, such as plastics, rubber.Metal projection outside inner tank body can be embedded in outer tank body inwall In neck, then coordinate magnetic absorption layer inside neck to strengthen fixing, avoid outer tank body and inner tank body from bouncing back, mitigate outer tank body and Restoring force between inner tank body is to the compressing in tank.Metal projection and neck can by existing metal working process with it is interior Tank body, outer tank body by integral forming, the magnetic absorption layer can be arranged on inside neck by any prior art.The outer tank body Lower end is provided with first connecting portion, and the base upper end is provided with second connecting portion, by between first connecting portion and second connecting portion Magnetic connection, further can effectively limit inner tank body, the glide direction of outer tank body, avoid it from deflecting and damage flexibility Seal, tank body sealing is caused to decline.Particularly, under normal operation, the magnetic of first connecting portion and second connecting portion is passed through Property absorption affinity, the gravity of outer tank body, the absorption affinity of metal projection and neck and its inwall so that can between outer tank body and inner tank body Reach the state of a stable equilibrium, will not shift.Particularly, company of the present invention in first connecting portion and second connecting portion Meet place and be provided with alarm device, when first connecting portion separates with second connecting portion, alarm device can warn, to remind operating personnel Pay attention to.

Further, the wall of the outer tank body is sequentially provided with metal outer wall, macromolecule hydrogel layer, rubber from outside to inside Layer, capillary fringe, aluminium foil layer, metal inner surface, the macromolecule hydrogel layer are high including the first macromolecule hydrogel layer and second Molecule hydrogel layer, the first macromolecule hydrogel layer, the second macromolecule hydrogel layer are symmetrically set in the rubber layer both sides, The capillary fringe penetrates the rubber layer and connects the first macromolecule hydrogel layer and the second macromolecule hydrogel layer, institute State rubber layer and be arranged with through hole, the metal inner surface is arranged with neck.Particularly, heretofore described capillary fringe, Rubber layer can realize that preferably metal capillary, the preferably larger rubber of coefficient of elasticity are made by this area any prior art For the rubber layer of the present invention；The macromolecule hydrogel layer is externally provided with macromolecule membrane parcel, and is sticked together by adhesive in height Molecule hydrogel layer surface, the macromolecule membrane can prevent the Water deep leaching of macromolecule hydrogel, evaporation.

Preferably, the rubber layer is thermoplastic elastomer, and the thermoplastic elastomer (TPE) is a kind of high-elastic with rubber Property, high intensity, high resilience, again have can injection molding feature material.With environment-protecting and non-poisonous safety, hardness range is wide, There are excellent coloring, soft-touch, weatherability, fatigue resistance and temperature tolerance, processing characteristics is superior, without vulcanization, can follow Ring use reduces cost, both can be with secondary injection molding, can also with the matrix material coating and bonding such as PP, PE, PC, PS, ABS It is separately formed, there is excellent elastic buffer performance.

The outer tank body of the present invention, by the metal outer wall that sets gradually, macromolecule hydrogel layer, rubber layer, capillary fringe, Aluminium foil layer, metal inner surface, the effect of damping, buffering can be reached, avoid the effect of external stress, change the internal pressure of the present invention, shadow Ring its stability.Macromolecule hydrogel layer of the invention by uniformly arranging, in outer tank body by the external stress from side When, the moisture in the first macromolecule hydrogel layer enters the second macromolecule hydrogel layer by capillary fringe, then leads to again Excess pressure difference, the moisture in the second macromolecule hydrogel layer returns to the first macromolecule hydrogel layer by capillary fringe, realizes Buffering.Synergistic compound rubber layer synergistic effect simultaneously, reaches optimal buffering effect.

Further, the metal inner surface is in 9%Ni steel inwall, Ni36 invar alloy inwall, 5083 aluminium alloy inwalls It is any.Particularly, inner tank body of the invention is prepared with metal inner surface using identical material.Pass through inner tank body of the present invention and gold Belong to the setting of inwall, stable austenitic state can be kept, resist the tank body chi as caused by high and low temperature environment and difference variation Very little change and bond open are dangerous, improve use and the Transport Safety of the present invention.

In the present invention, the 9%Ni steel inwalls of use, wherein 9%Ni steel are a kind of low-carbon quenched and tempered steel containing higher Ni contents, In -196 DEG C of minimum temperature in use, it remains to keep good obdurability and high intensity.Heat treatment mode mainly has following 3 Kind:Normalizing-normalizing-tempering (NNT), finally give tempered martensite and bainite structure, quenching-tempering (QT) and quenching-Asia Warm quenching-tempering (IHT) can obtain low-carbon tempered martensite.Chemical composition and mechanical property of the country variant to 9%Ni steel It can require to be slightly different, its cryogenic mechanics performance depends primarily on its chemical composition, the degree of purity of steel and institutional framework etc. Aspect, the temperature difference it is larger, especially there is excellent stability under low-temperature condition, improve the safety in utilization of the present invention.

The Ni36 invar alloy inwalls of use, wherein Ni36 invar alloy is the face-centred cubic structure containing 36%Ni, is one Kind iron-based Langaloy, its function admirable, there is relatively low hot expansibility, temperature changes, and its size is basically unchanged, compared Relatively stable, average coefficient of expansion is 1.6 × 10 under normal temperature^-6/ DEG C, Ni contents be 36% Ni-Fe invar alloy temperature as little as- Stable austenitic state is maintained at 273 DEG C.The performance of invar alloy makes it when being applied under transporting low temperature environment The security of transport is substantially increased, the tank body of invar alloy material can be resisted to be caused by high and low temperature environment and difference variation Tank body size change and bond open is dangerous, improve the safety in utilization of the present invention.

5083 aluminium alloy inwall in the present invention, wherein the aluminium used has face-centred cubic structure, with other metal phase ratios, temperature Degree declines, and its mechanical property does not decline, strengthened to a certain extent on the contrary, no black brittleness, therefore aluminum alloy materials are each Industry is used widely.The aluminium alloy of used at ultra-low temperature 5083 is to be applied to one of low-temperature storage tank material, and the alloy has good Plasticity, higher intensity, corrosion resistance and the advantages that processability, the security for improving the transport of the present invention and using.

Further, the base include body, the symmetrically arranged second connecting portion in body upper end, the body include according to Arlt's strata, cushion, antibiotic layer, waterproof layer, the bottom plate of secondary setting.

The base of the present invention, used arlt's strata are to be interweaved by the string of prior art with organic fiber The tridimensional network of formation, there is high malleable.The antibiotic layer is Fe of the prior art₃O₄- graphene composite antibacterial Layer, the waterproof layer is polyurethane water-proof layer of the prior art, and the bottom plate is high rigidity timber of the prior art or wood Foundation board.

The base of the present invention, by the effect of arlt's strata and cushion, there is excellent antidetonation to buffer, while this hair The bottom plate of bright use is netted due to high rigidity timber of the present invention or wood-fibred base plate interior compared with the metallic plate of prior art Fibre structure and there is certain cushioning effect；, being capable of anti-corrosion protection against the tide by the effect of antibiotic layer and waterproof layer；In view of this hair Bright portable performance, base of the present invention have the advantages of in light weight, will not cause extra burden.

Further, the cushion includes EPE plates, the first projection, the second projection, and first projection is located at described On EPE plates, on the EPE plates, first projection is staggeredly symmetrically set second projection in opposite directions with second projection Put.Particularly, the end of first projection is provided with spacing, the end of second projection with the EPE plates where the second projection Offseted with the EPE plates described in first projection.In the present invention, EPE plates of the prior art, EPE are used （ExpandablePolyethylene）, it is expandability polyethylene, also known as pearl cotton, is non-crosslinked hole-closing structure, it is with low Density polyethylene（LDPE）For the high foamed polyethylene product of main material extruding generation.It is by low density polyethylene (LDPE) fat through physics Foaming produces countless separated foams and formed.The shortcomings that overcoming frangible common foamed glue, deformation, recovery difference.With water proof Moistureproof, shockproof, sound insulation, insulation, plasticity is good, toughness is strong, reconstituted circularly, environmental protection, strong crashworthiness power many advantages, such as, also have Good chemical resistance.

Further, the horizontal cross-section of first projection is circular arc, and the horizontal cross-section of second projection is circular arc, institute The radian for stating the first projection is 1.5-2.2 times of the second projection radian.

In the present invention, first projection, the second projection, EPE plates are prepared using the material of phase same material, the buffering of use Layer, can be in the feelings by external stress by the effect of the first projection and the second projection of the different volumes that are staggered in opposite directions Elastic deformation occurs under condition, the longitudinal stress being subject to is moved to laterally expansion, and realizes resilience, so as to reach the effect of damping, buffering Fruit.

Further, described its raw material of Macromolecule glue-line by weight include 2-6 part diallyl carbonic acid diethylene glycol (DEG)s ester, 4-12 part acryloyls proline methyl ester, 0.01-0.19 parts non-ionic celluloses ether, 0.5-2 parts maleic anhydride, 0.3- 1.5 parts of potassium peroxydisulfates, 0.01-0.07 parts trihydroxy glutaric acid, 0.02-0.05 parts cerium hydroxide and the pure water of 90-100 parts. In the present invention, the raw material such as the diallyl carbonic acid diethylene glycol (DEG) ester, acryloyl proline methyl ester can be selected prior art and realize. Non-ionic celluloses ether can be any one or mixing in HPMC ether, hydroxypropylcelluloether ether.Along fourth Enedioic acid acid anhydride also known as maleic anhydride or apple anhydride dehydration, normal abbreviation cis-butenedioic anhydride.Potassium peroxydisulfate, inorganic compound, white crystals.It is suitable Anhydride maleique and potassium peroxydisulfate can promote the polymerization of diallyl carbonic acid diethylene glycol (DEG) ester and non-ionic celluloses ether, hand over Connection, be allowed to pure water with reference to and form hydrogel.Trihydroxy glutaric acid（3-Hydroxy glutaric acid）It is generally used for having Machine synthesizes and medicine intermediate, and commercially available prod can be selected and realize.Cerium hydroxide is usually used in doing the principle of cerous nitrate synthesis.The present invention In, trihydroxy glutaric acid and cerium hydroxide are especially added with, in the presence of the two, in macromolecule hydrogel when can improve moisture low temperature Stability in system, and water outlets are largely discharged at 50 DEG C or so, to realize fast cooling, and there is certain buffering effect.

Further, its raw material of the flexible seals includes 70-90 parts polyvinyl chloride by weight, 0.1-0.3 parts are received Rice aluminum oxide, 2-6 parts three (dimethylamino) silane, 1-10 part coupling agents.

Polyvinyl chloride, English abbreviation PVC（Polyvinyl chloride), it is VCM（vinyl chloride Monomer, abbreviation VCM）In initiators such as peroxide, azo-compounds；It is or anti-by radical polymerization under light, heat effect Answer the polymer that mechanism is polymerized.The preferred γ crystal formations of nano aluminium oxide, the preferred 10-50nm of particle diameter.Three (dimethylamino) Silane（CAS is numbered：15112-89-7）Commercially available prod can be selected to realize.The coupling agent can be selected prior art and realize, act as With the combined strength bination for being enhancing nano aluminium oxide and polyvinyl chloride.It is of the invention especially to select polyvinyl chloride as the flexible sealing Part, flexible seals easily ftracture after bending state is chronically at because of abrasion, influence air-tightness.Present invention addition is appropriate Nano aluminium oxide and three (dimethylamino) silane be packed into polyvinyl chloride, the anti-wear performance of flexible seals can be obviously improved, Its flexibility is not significantly reduced, so as to improve the reliability of the present invention.

Knot with cushioning effect of the invention by being set between liquid nitrogen plan, outer case, internal box and outer case Structure, can provide for transport, carrying and use of the present invention and stably ensure, avoiding liquid nitrogen plan, internal pressure is not caused by vibrations are rocked The potential safety hazard such as stable.The freezing mode of the refrigerating box of the present invention can make temperature field in internal box more uniform, using use Liquid nitrogen is as refrigerated source so that internal box contains the nitrogen of higher concentration, can play preferable controlled atmosphere effect.

The present invention provides detection sample stored frozen device, safety and stability, is convenient for carrying, is easy to use, is adapted to out of doors Cold storage container in sampling process as collection sample, the present invention can preserve sample in the case where being not connected to power supply 1-7 days.

Brief description of the drawings

Fig. 1 is the structural representation of the present invention；

Fig. 2 is the local structure schematic diagram of the present invention；

Fig. 3 is the local structure schematic diagram of the present invention；

Fig. 4 is the local structure schematic diagram of the present invention；

Fig. 5 is the local structure schematic diagram of the present invention；

Fig. 6 is the local structure schematic diagram of the present invention；

Fig. 7 is the local structure schematic diagram of the present invention；

Fig. 8 is the local structure schematic diagram of the present invention.

Embodiment

For the ease of it will be appreciated by those skilled in the art that making below in conjunction with accompanying drawing and embodiment to the present invention further detailed Thin description：

Embodiment 1

One kind detection sample stored frozen device, including casing, freeze proof flexible pipe 4, tell that casing includes outer case 1 and internal box 2, The internal box 2 is inside the outer case 1, it is characterised in that the outer case 1 is additionally provided with liquid nitrogen plan 3, the liquid nitrogen Device 3 is located at the side of the internal box 2；

The outer case 1 is provided with outer case lid 11, and the outer inner side of case lid 11 is symmetrical to be staggeredly provided with elastomer 12 and magnetic block 13；

The internal box 2 is provided with interior case lid 20, and the elastomer 12 offsets with the interior outside of case lid 20, the magnetic block 13 with The outside of interior case lid 20 connects；

The tank wall of the internal box 2 is provided with heat-insulation layer 21, waterproof layer 22, and the waterproof layer 22 is symmetrically set in 21 liang of the heat-insulation layer Side, the interior case lid 20 are sequentially provided with metal level 25, heat-insulation layer 21, waterproof layer 22, the first hollow sandwich 23, the tank floor Be additionally provided with the second hollow sandwich 24, the liquid nitrogen plan 3 by freeze proof flexible pipe 4 respectively with first hollow sandwich 23, second Hollow sandwich 24 connects；The structure of internal box 2 can provide an effective thermal environment for collection sample in the present invention, simultaneously With preferable water resistance.

It in the present invention, can be realized using elastomer 12 and magnetic block 13 by any prior art, pass through the He of elastomer 12 Outer case 1 and internal box 2 are fitted close by magnetic block 13 in upper end, after outer case lid 11 are closed, the elastomer 12 with it is described The interior surface of case lid 20 offsets, and can have certain cushioning effect to the stress of tank surface；The magnetic block 13 can be with internal box 2 Metal level 25 adsorb, limit internal box 2 position, avoid internal box 2 shift influence outer case 1 inside monolithic stability Property.

The freeze proof flexible pipe is provided with the first plenum member 41, the freeze proof flexible pipe and institute with the first hollow sandwich junction State the second hollow sandwich junction and be provided with the second plenum member 42；

First hollow sandwich 23 is arranged with spray orifice 25, and second hollow sandwich 24 is arranged with spray orifice 25；

Particularly, freeze proof flexible pipe 4 of the invention can be realized by this area any prior art, and can be incited somebody to action by prior art The freeze proof flexible pipe 4 is provided in the heat-insulation layer 21 of the side tank wall of the internal box 2, then respectively with first hollow sandwich 23rd, the second hollow sandwich 24 connects.

The horizontal cross-section of the spray orifice 25 is isosceles trapezoid.The shape of the spray orifice 25 can effectively improve the gas of bottom flow Pressure so that freezing is more uniform.

Particularly, the outer case lid 11 realizes that folding connects by hinge arrangement of the prior art, the outer case lid 11 The sealing strip of prior art is provided with the junction of outer case 1, strengthens sealing property；The interior case lid 20 is by of the prior art Hinge arrangement realizes that folding connects, and the interior case lid 20 is provided with the sealing strip of prior art, enhancing sealing with the junction of internal box 2 Performance.

The outer case bottom 14 includes damp-proof layer 141, heat-insulated cushion 142, the first magnet layer 143, the second magnet layer 145th, elastomer layer 144, wearing layer 146, the first magnet layer are located at the side of the elastomer layer, the second magnet layer Located at the opposite side of the elastomer layer, the heat-insulated cushion is respectively arranged on the first magnet layer, the one of the second magnet layer Side, the damp-proof layer are located at the side of the heat-insulated cushion, and the wearing layer is located at the side of the heat-insulated cushion and position In the lower end of the outer case bottom.

The Fluid pressure that first plenum member 41 exports is the 0.68 of the Fluid pressure that second plenum member 42 exports Times.

The first magnet layer 143 and the second magnet layer 145 are Permanent-magnet layer, the first magnet layer and the second magnet The magnetic of layer is repelled each other.

Particularly, the outer case 1 is provided with heat insulating coat provided with heat-insulated outside, and the heat insulating coat is SiO₂Aeroge every Hot coating, the SiO₂Aerogel heat-proof coating can be realized by any prior art.The heat of outside can effectively be completely cut off, kept away Exempt from that heat transfer occurs with box house, effectively extend the insulation time limit.

The liquid nitrogen plan 3 includes tank body, and the tank body is provided with exhaust outlet, and the freeze proof flexible pipe 4 is connected with exhaust outlet, The exhaust outlet is provided with valve member, and the valve member includes the components such as atmospheric valve, pressure charging valve, can be any existing by this area There is technology realization.The tank body lower end is provided with base, and the tank body includes outer tank body, inner tank body, and the inner tank body is located at outer tank Internal portion, it is characterised in that the outer tank body lower ending opening, the inner tank body upper end open, the tank body are interior provided with flexible close Sealing, one end of the flexible seals and the upper end of inner tank body connect, in the other end and outer tank body of the flexible seals The upper end connection of side；

Particularly, the flexible seals are coaxially disposed with the tank body.

Further, the flexible seals are the hollow tube-shape seal of side indent.

Further, the wall of the outer tank body is sequentially provided with metal outer wall, macromolecule hydrogel layer, rubber from outside to inside Layer, capillary fringe, aluminium foil layer, metal inner surface, the macromolecule hydrogel layer are high including the first macromolecule hydrogel layer and second Molecule hydrogel layer, the first macromolecule hydrogel layer, the second macromolecule hydrogel layer are symmetrically set in the rubber layer both sides, The capillary fringe penetrates the rubber layer and connects the first macromolecule hydrogel layer and the second macromolecule hydrogel layer, institute State rubber layer and be arranged with through hole 53, the metal inner surface is arranged with neck.Particularly, heretofore described capillary Layer, rubber layer can be realized by this area any prior art, preferably metal capillary, the preferably larger rubber of coefficient of elasticity Rubber layer as the present invention；The macromolecule hydrogel layer is externally provided with macromolecule membrane parcel, and is sticked together by adhesive Macromolecule hydrogel layer surface, the macromolecule membrane can prevent the Water deep leaching of macromolecule hydrogel, evaporation.

Preferably, the rubber layer is thermoplastic elastomer 143, and the thermoplastic elastomer (TPE) 12 is a kind of with rubber High resiliency, high intensity, high resilience, again have can injection molding feature material.With environment-protecting and non-poisonous safety, hardness range Extensively, there are excellent coloring, soft-touch, weatherability, fatigue resistance and temperature tolerance, processing characteristics is superior, can be with without vulcanization Recycling reduces cost, both with the matrix material coating and bonding such as PP, PE, PC, PS, ABS, can also may be used with secondary injection molding With separately formed, there is excellent elastic buffer performance.

Further, the metal inner surface is 9%Ni steel inwalls.Particularly, inner tank body of the invention uses with metal inner surface It is prepared by identical material.By the setting of inner tank body of the present invention and metal inner surface, stable austenitic state, resistance can be kept The tank body size as caused by high and low temperature environment and difference variation changes and bond open is dangerous, improves using and transporting for the present invention Defeated security.

Further, the base include body, the symmetrically arranged second connecting portion in body upper end, the body include according to Arlt's strata, cushion, antibiotic layer, waterproof layer 22, the bottom plate of secondary setting.

The base of the present invention, used arlt's strata are to be interweaved by the string of prior art with organic fiber The tridimensional network of formation, there is high malleable.The antibiotic layer is Fe of the prior art₃O₄- graphene composite antibacterial Layer, the waterproof layer 22 is polyurethane water-proof layer 22 of the prior art, and the bottom plate is high rigidity timber of the prior art Or wood-fibred bottom plate.

The base of the present invention, by the effect of arlt's strata and cushion, there is excellent antidetonation to buffer, while this hair The bottom plate of bright use is netted due to high rigidity timber of the present invention or wood-fibred base plate interior compared with the metallic plate of prior art Fibre structure and there is certain cushioning effect；, being capable of anti-corrosion protection against the tide by the effect of antibiotic layer and waterproof layer 22；In view of this The portable performance of invention, base of the present invention have the advantages of in light weight, will not cause extra burden.

Further, the horizontal cross-section of first projection is circular arc, and the horizontal cross-section of second projection is circular arc, institute The radian for stating the first projection is 2 times of the second projection radian.

The present invention has cushioning effect by what is set between liquid nitrogen plan 3, outer case 1, internal box 2 and outer case 1 Structure, can for the present invention transport, carrying and use provide stably ensure, avoid liquid nitrogen plan 3 caused by vibrations are rocked The potential safety hazards such as internal pressure is unstable.The freezing mode of the refrigerating box of the present invention can make in internal box 2 that temperature field is more uniform, adopt By the use of using liquid nitrogen as refrigerated source so that nitrogen of the internal box 2 containing higher concentration, preferable controlled atmosphere effect can be played.

The present invention provides detection sample stored frozen device, safety and stability, is convenient for carrying, is easy to use, is adapted to out of doors Cold storage container in sampling process as collection sample.

Embodiment 2

The Fluid pressure that first plenum member 41 exports is the 0.75 of the Fluid pressure that second plenum member 42 exports Times.

Particularly, the flexible seals are coaxially disposed with the tank body.

Further, the flexible seals are the hollow tube-shape seal of side indent.

Further, the metal inner surface 316 is Ni36 invar alloy inwalls.

Further, the horizontal cross-section of first projection is circular arc, and the horizontal cross-section of second projection is circular arc, institute The radian for stating the first projection is 2.2 times of the second projection radian.

Embodiment 3

The Fluid pressure that first plenum member 41 exports is the 0.5 of the Fluid pressure that second plenum member 42 exports Times.

Particularly, the flexible seals are coaxially disposed with the tank body.

Further, the flexible seals are the hollow tube-shape seal of side indent.

Further, the metal inner surface 316 is 5083 aluminium alloy inwalls.5083 aluminium alloy inwall in the present invention, wherein The aluminium of use has face-centred cubic structure, and with other metal phase ratios, temperature declines, and its mechanical property does not decline, on the contrary one Determine to strengthen in degree, no black brittleness, therefore aluminum alloy materials are used widely in every profession and trade.The aluminium alloy of used at ultra-low temperature 5083 It is to be applied to one of low-temperature storage tank material, the alloy has good plasticity, higher intensity, corrosion resistance and processability etc. excellent Point, the security for improving the transport of the present invention and using.

Further, the horizontal cross-section of first projection is circular arc, and the horizontal cross-section of second projection is circular arc, institute The radian for stating the first projection is 1.5 times of the second projection radian.

Embodiment 4

The present embodiment provides a kind of detection sample stored frozen device, and the present embodiment structure is consistent with embodiment 2, different It is：Further, described its raw material of Macromolecule glue-line includes 4 parts of diallyl carbonic acid diethylene glycol (DEG) esters, 6 parts of propylene by weight Acyl proline methyl ester, 0.07 part of non-ionic celluloses ether, 1.2 parts of maleic anhydrides, 0.9 part of potassium peroxydisulfate, 0.03 part three Hydroxyl glutaric acid, 0.04 part of cerium hydroxide and 95 parts of pure water.In the present invention, the diallyl carbonic acid diethylene glycol (DEG) ester, third The raw materials such as alkene acyl proline methyl ester can be selected prior art and realize.Non-ionic celluloses ether can be HPMC Any one in ether, hydroxypropylcelluloether ether or mixing.Maleic anhydride also known as maleic anhydride or apple anhydride dehydration, often Abbreviation cis-butenedioic anhydride.Potassium peroxydisulfate, inorganic compound, white crystals.Maleic anhydride and potassium peroxydisulfate can promote diallyl The polymerization of carbonic acid diethylene glycol (DEG) ester and non-ionic celluloses ether, crosslinking, be allowed to pure water with reference to and form hydrogel.Trihydroxy penta Diacid（3-Hydroxy glutaric acid）It is generally used for organic synthesis and medicine intermediate, commercially available prod can be selected and realize. Cerium hydroxide is usually used in doing the principle of cerous nitrate synthesis.In the present invention, trihydroxy glutaric acid and cerium hydroxide are especially added with, the two In the presence of, stability when can improve moisture low temperature in macromolecule hydrogel system, and largely discharged at 50 DEG C or so Water, to realize fast cooling, and there is certain buffering effect.

Further, its raw material of the flexible seals by weight include 82 parts of polyvinyl chloride, 0.24 part it is nano oxidized Aluminium, 4 part of three (dimethylamino) silane, 6 parts of coupling agents.

The preparation method of the macromolecule hydrogel layer of the present embodiment is：HPMC and diallyl carbonic acid two Glycol ester is dissolved in pure water, then adds potassium peroxydisulfate, is filled with nitrogen, and temperature rises to 41 DEG C, and preheating adds suitable after 15 minutes Anhydride maleique, stirring reaction 8 hours, then add acryloyl proline methyl ester（The degree of polymerization is 1950）After being completely dissolved, Pour into and household freezer is put into mould（-15℃）Middle freezing is thawed after 2.5 hours, is circulated 5 times, secure satisfactory grades sub- hydrogel layer.

Preferably, in the present embodiment, coupling agent selects excellent aluminate coupling agent.In the present embodiment, flexible seals can incite somebody to action After each raw materials melt mixing, injection is formed.

Embodiment 5

The present embodiment provides a kind of detection sample stored frozen device, and the present embodiment structure is consistent with embodiment 2, unlike： Described its raw material of macromolecule hydrogel layer includes 3 parts of diallyl carbonic acid diethylene glycol (DEG) esters, 8 parts of acryloyl proline first by weight Ester, 0.07 part of non-ionic celluloses ether, 1 part of maleic anhydride, 0.9 part of potassium peroxydisulfate, 0.04 part of trihydroxy glutaric acid, 0.03 part of cerium hydroxide and 96 parts of pure water.

Preferably, its raw material of polyvinyl chloride seal described in the present embodiment by weight include 85 parts of polyvinyl chloride, 0.21 part of nano aluminium oxide, 3 part of three (dimethylamino) silane, 5 parts of coupling agents.In the present embodiment, coupling agent selects excellent Aluminate even Join agent.In the present embodiment, after flexible seals can mix each raw materials melt, injection is formed.

Embodiment 6

The present embodiment provides a kind of macromolecule hydrogel layer for the structure of embodiment 2.Its raw material of hydrogel layer wraps by weight Include 3 parts of diallyl carbonic acid diethylene glycol (DEG) esters, 5 parts of acryloyl proline methyl esters, 0.18 part of non-ionic celluloses ether, 0.6 part it is suitable Anhydride maleique, 0.4 part of potassium peroxydisulfate, 0.06 part of trihydroxy glutaric acid, 0.04 part of cerium hydroxide and 99 parts of pure water.

Embodiment 7

The present embodiment provides a kind of macromolecule hydrogel layer for the structure of embodiment 3.Its raw material of hydrogel layer wraps by weight Include 5 parts of diallyl carbonic acid diethylene glycol (DEG) esters, 11 parts of acryloyl proline methyl esters, 0.02 part of non-ionic celluloses ether, 2 parts of suitable fourths Enedioic acid acid anhydride, 1.4 parts of potassium peroxydisulfates, 0.06 part of trihydroxy glutaric acid, 0.02 part of cerium hydroxide and 90 parts of pure water.

Embodiment 8

The present embodiment provides a kind of macromolecule hydrogel layer for the structure of embodiment 3.Its raw material of hydrogel layer wraps by weight Include 6 parts of diallyl carbonic acid diethylene glycol (DEG) esters, 12 parts of acryloyl proline methyl esters, 0.01 part of non-ionic celluloses ether, 0.5-2 parts Maleic anhydride, 0.3 part of potassium peroxydisulfate, 0.07 part of tartronic acid, 0.05 part of cerium hydroxide and 100 parts of pure water.

Embodiment 9

The present embodiment provides a kind of macromolecule hydrogel layer for the structure of embodiment 1.Its raw material of hydrogel layer wraps by weight Include 2 parts of diallyl carbonic acid diethylene glycol (DEG) esters, 4 parts of acryloyl proline methyl esters, 0.19 part of non-ionic celluloses ether, 0.5 part it is suitable Anhydride maleique, 1.5 parts of potassium peroxydisulfates, 0.01 part of trihydroxy glutaric acid, 0.05 part of potassium hydroxide and 100 parts of pure water.

Embodiment 10

The present embodiment provides a kind of polyvinyl chloride seal for the structure of embodiment 2, and its raw material includes 90 parts and gathered by weight Vinyl chloride, 0.3 part of nano aluminium oxide, 2 part of three (dimethylamino) silane, 10 parts of coupling agents.In the present embodiment, coupling agent is silane Coupling agent.

Embodiment 11

The present embodiment provides a kind of polyvinyl chloride seal for the structure of embodiment 2, and its raw material includes 80 parts and gathered by weight Vinyl chloride, 0.1 part of nano aluminium oxide, 6 part of three (dimethylamino) silane, 1 part of coupling agent.

Embodiment 12

The present embodiment provides a kind of polyvinyl chloride seal for the structure of embodiment 2, and its raw material includes 90 parts and gathered by weight Vinyl chloride, 0.3 part of nano aluminium oxide, 10 parts of coupling agents.

Embodiment 13

The present embodiment provides a kind of polyvinyl chloride seal for the structure of embodiment 2, and its raw material is commercially available polyvinyl chloride.

Flexible seals wearability test.

Table 1.

Experimental group	Coefficient of friction（Steel）	Elongation at break（%）
			Embodiment 4	0.14	443
Embodiment 5	0.15	448
			Embodiment 10	0.11	481
Embodiment 11	0.16	446
			Embodiment 12	0.28	459
Embodiment 13	0.26	475

Macromolecule hydrogel moisture holding capacity is tested

The macromolecule hydrogel of embodiment 4- embodiments 9 is chosen, is prepared into the cm of 20 cm × 10 shape（Water content is 100g）.Macromolecule hydrogel is heated with 40 DEG C, 45 DEG C, 50 DEG C of temperature respectively, records the mass change in its 12 minutes.Its As a result as shown in Table 2-4.

The macromolecule hydrogel mass change value of table 2.（40℃）

Experimental group/mass change（g）	3min	6 min	9 min	12 min
					Embodiment 4	-0.7	-1.3	-1.5	-1.5
Embodiment 5	-1.4	-1.7	-2.0	-2.1
					Embodiment 6	-0.3	-0.8	-1.3	-2.2
Embodiment 7	-1.7	-2.9	-5.9	-12.1
					Embodiment 8	-2.0	-3.3	-7.3	-10.6
Embodiment 9	-1.7	-3.0	-6.1	-11.3

The macromolecule hydrogel mass change value of table 3.（45℃）

Experimental group/mass change（g）	3min	6 min	9 min	12 min
					Embodiment 4	-0.8	-1.4	-1.5	-1.7
Embodiment 5	-1.4	-2.0	-2.2	-2.9
					Embodiment 6	-1.2	-2.0	-2.4	-3.3
Embodiment 7	-2.0	-3.3	-7.0	-13.4
					Embodiment 8	-2.1	-3.8	-10.7	-15.9
Embodiment 9	-1.8	-3.6	-8.7	-14.4

The macromolecule hydrogel mass change value of table 4.（50℃）

Experimental group/mass change（g）	3min	6 min	9 min	12 min
					Embodiment 4	-12.8	-15.9	-23.4	-30.6
Embodiment 5	-10.9	-17.2	-22.6	-30.0
					Embodiment 6	-12.5	-19.0	-27.6	-31.3
Embodiment 7	-2.8	-5.8	-11.0	-15.1
					Embodiment 8	-4.5	-8.3	-12.5	-18.4
Embodiment 9	-3.4	-6.3	-11.4	-16.6

It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, and do not carrying on the back In the case of spirit or essential attributes from the present invention, the present invention can be realized in other specific forms.Therefore, no matter from which From the point of view of a bit, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention will by appended right Ask rather than described above limits, it is intended that all changes in the implication and scope of the equivalency of claim will be fallen Include in the present invention.Any reference in claim should not be considered as to the involved claim of limitation.

Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art It is appreciated that other embodiment.It is noted that the technical characteristic not being described in detail in the present invention, can be by appointing One prior art is realized.

Claims

1. one kind detection sample stored frozen device, including casing, freeze proof flexible pipe, tell that casing includes outer case and internal box, The internal box is inside the outer case, it is characterised in that the outer case is additionally provided with liquid nitrogen plan, the liquid nitrogen plan Located at the side of the internal box；

The tank wall of the internal box is provided with heat-insulation layer, waterproof layer, and the waterproof layer is symmetrically set in the heat-insulation layer both sides, described interior Case lid is sequentially provided with metal level, heat-insulation layer, waterproof layer, the first hollow sandwich, and the tank floor is additionally provided with the second hollow sandwich, The liquid nitrogen plan is connected with first hollow sandwich, the second hollow sandwich respectively by freeze proof flexible pipe；

The freeze proof flexible pipe is provided with the first plenum member, the freeze proof flexible pipe and described second with the first hollow sandwich junction Hollow sandwich junction is provided with the second plenum member；

The outer case bottom includes damp-proof layer, heat-insulated cushion, the first magnet layer, the second magnet layer, elastomer layer, wear-resisting Layer, the first magnet layer are located at the side of the elastomer layer, and the second magnet layer is another located at the elastomer layer Side, the heat-insulated cushion are respectively arranged on the first magnet layer, the side of the second magnet layer, the damp-proof layer located at it is described every The side of Heat buffered layer, the wearing layer is located at the side of the heat-insulated cushion and positioned at the lower end of the outer case bottom.

2. detection sample stored frozen device according to claim 1, it is characterised in that the first plenum member outlet Fluid pressure is 0.5-0.75 times of the Fluid pressure of the second plenum member outlet.

3. the first magnet layer and the second magnet layer are Permanent-magnet layer, the magnetic of the first magnet layer and the second magnet layer Repel each other.

4. detection sample stored frozen device according to claim 1, it is characterised in that the horizontal cross-section of the spray orifice is Isosceles trapezoid.

5. detection sample stored frozen device according to claim 1, it is characterised in that the liquid nitrogen plan includes tank Body, the tank body are provided with exhaust outlet, and the exhaust outlet is provided with valve member, and the tank body lower end is provided with base, the tank body Including outer tank body, inner tank body, the inner tank body is inside outer tank body, it is characterised in that the outer tank body lower ending opening, it is described Inner tank body upper end open, the tank body is interior to be provided with flexible seals, and one end of the flexible seals and the upper end of inner tank body connect Connect, the upper end connection on the inside of the other end and outer tank body of the flexible seals；

The internal face of the outer tank body is arranged with neck, and the neck is provided with magnetic absorption layer, the outer wall of the inner tank body Face is arranged with metal barb, and the neck is connected with metal barb.

6. detection sample stored frozen device according to claim 5, it is characterised in that the flexible seals are side The hollow tube-shape seal of indent.

7. detection sample stored frozen device according to claim 5, it is characterised in that the wall of the outer tank body is by outer Metal outer wall, macromolecule hydrogel layer, rubber layer, capillary fringe, aluminium foil layer, metal inner surface, the high score are sequentially provided with to interior Sub- hydrogel layer includes the first macromolecule hydrogel layer and the second macromolecule hydrogel layer, the first macromolecule hydrogel layer, Second macromolecule hydrogel layer is symmetrically set in the rubber layer both sides, and the capillary fringe penetrates the rubber layer by described first Macromolecule hydrogel layer and the connection of the second macromolecule hydrogel layer, the rubber layer are arranged with through hole, the metal inner surface pair Title is provided with neck.

8. detection sample stored frozen device according to claim 7, it is characterised in that the metal inner surface is 9%Ni steel Any of inwall, Ni36 invar alloy inwall, 5083 aluminium alloy inwalls.

9. detection sample stored frozen device according to claim 8, it is characterised in that the base includes body, sheet The symmetrically arranged second connecting portion in body upper end, it is arlt's strata that the body includes setting gradually, cushion, antibiotic layer, anti- Water layer, bottom plate.

10. detection sample stored frozen device according to claim 9, it is characterised in that the cushion includes EPE Plate, the first projection, the second projection, on the EPE plates, second projection is located on the EPE plates first projection, First projection is staggeredly symmetrical arranged in opposite directions with second projection.