CN107624515A - Black fungus open air cultivating bacteria bag and its preparation and application method - Google Patents
Black fungus open air cultivating bacteria bag and its preparation and application method Download PDFInfo
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- CN107624515A CN107624515A CN201711002984.7A CN201711002984A CN107624515A CN 107624515 A CN107624515 A CN 107624515A CN 201711002984 A CN201711002984 A CN 201711002984A CN 107624515 A CN107624515 A CN 107624515A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 98
- 241000233866 Fungi Species 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 229920001903 high density polyethylene Polymers 0.000 claims description 23
- 239000004700 high-density polyethylene Substances 0.000 claims description 23
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims description 14
- 239000003795 chemical substances by application Substances 0.000 claims description 14
- 239000002361 compost Substances 0.000 claims description 12
- 230000035784 germination Effects 0.000 claims description 10
- 229920006124 polyolefin elastomer Polymers 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 8
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- 239000007822 coupling agent Substances 0.000 claims description 6
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 4
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- 235000015099 wheat brans Nutrition 0.000 claims description 4
- 239000002023 wood Substances 0.000 claims description 4
- 235000018324 Shepherdia canadensis Nutrition 0.000 claims description 3
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- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of black fungus open air cultivating bacteria bag and its preparation and application method, the bacterium bag includes one bag of body, and this bag of middle side part of body one has the hyalomere for being used for observing the internal mycelial growth situation of bag, and remainder is non-transparent.The outdoor cultivation of black fungus is carried out using bacterium bag of the present invention, it can be reflected by light, light shield, light convert, the multiple action of light directional induction, avoid loss of the strong illumination to mycelia, and the bacterium bag is resistant to autoclave sterilization, and with good weatherability properties, pliability, the separation of bag rod can effectively be avoided, so as to realize black fungus is high-quality, efficiently produce while, prevent the generation of wall mushroom and " setting sun disease ".
Description
Technical field
Present invention relates particularly to a kind of black fungus open air cultivating bacteria bag and its preparation and application method.
Background technology
At present, the bacterium bag of polyethylene or polypropylene material, but polyethylene non-refractory are mainly used in edible fungus culturing, only
Normal-pressure sterilization can be used, though and polypropylene can high temperature resistant, pliability is poor, broken.And due to traditional the quality of the bag and physics
Performance does not reach the index request of " soft, tough, bullet ", it is being carried, in transport and cargo handling process, is easily made because colliding, extruding etc.
Deformed into bacteria stick and cause a bag rod separation, easily grow wall mushroom；Simultaneously as bacterium bag used at present is monochromatic transparent plastic
Bag, no shaded effect, also easily because sunlight is uneven, and part mycelia vernalization, quick-fried flower bud in bag is set to form wall mushroom.The opposing party
Face, due to transparent bacterium bag can not shield lights, long-time strong illumination can make bacteria stick temperature too high, mycelia burnt, burnt and
Occur " setting sun disease "；And if the separation of bag rod produces space, or watering is not drenched, and part " steaming Saunas " can be formed after strong illumination
Phenomenon, further aggravate " setting sun disease ".
Black fungus（Auriculuria auricula）For the precious medicinal and edible Jelly fungi in China, it is rich in albumen
Matter, fat, carbohydrate and multivitamin, there is moistening lung, clear stomach etc. of washing to act on, it is deep to be liked by numerous people.But black fungus exists
Wall mushroom for occurring in outdoor cultivation and " setting sun disease " etc. cause harm, the strong influence yield and quality of black fungus.Therefore,
High-quality, the high-yield culturing of black fungus how are realized, is urgent problem to be solved in current black fungus production.
The content of the invention
For problem above, the invention provides one kind to be resistant to autoclave sterilization, has good weatherability properties, pliability,
And have the black fungus open air cultivating bacteria bag of its unique texture, the outdoor cultivation of black fungus is used it for, can not only realize black fungus
High-quality, efficient production, can also prevent the generation of wall mushroom and " setting sun disease ".
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of black fungus open air cultivating bacteria bag, including one bag of body, this bag of middle side part of body one, which has, to be used to observe the internal mycelia life of bag
The hyalomere of long situation, remainder is non-transparent, to prevent the internal portion of bag by illumination.
For bag body hyalomere in strip through bag body head and the tail, its width is 1 ~ 2cm.
The non-transparent part of bag body is white, grey or black, preferably grey.
Bag body one end closing.
The non-transparent part of bacterium bag is made up of the raw material of following percetage by weight：High density polyethylene (HDPE)（HPDE）50%th, gather
Olefin elastomer（POE）20%th, titanium dioxide 15%, light stabilizer 2%, light conversion agent 1%, antioxidant 1.5%, dispersant 3.5%, reflective agent
2%th, coupling agent 1.5%, toughener 3.5%；
The bacterium bag hyalomere is mainly made up of the raw material of following percetage by weight：High density polyethylene (HDPE)（HPDE）75%th, it is extremely low close
Spend polyethylene（LLD）15%th, polyolefin elastomer（POE）10%.
The preparation method of the bacterium bag is by high density polyethylene (HDPE), polyolefin elastomer, titanium dioxide, light stabilizer, turns light
Agent, antioxidant, dispersant, reflective agent, coupling agent, toughener are mixed to prepare component A through homogenizer in proportion, by high density
Polyethylene, very low density polyethylene and polyolefin elastomer are after homogenizer is mixed to prepare component B；Component A, B is distinguished
Through dual-screw pelletizer extruding pelletization, then using the blowout shaping of double-colored inflation film machine, then sealed through automatic notch cutter, i.e.,
；The technological parameter of the double-colored inflation film machine is：210 DEG C of die head, 200 DEG C of connector, homogenize 180 DEG C of section, compression section 175
DEG C, 155 DEG C of feeding section.
Illumination is to influence black fungus to grow most direct one of most important factor, especially at 11 points at noon to afternoon 4
In this period of point, the irradiation of strong sunshine is the main reason for triggering setting sun disease, therefore, it is necessary to be reflected as far as possible
Fall, reduce luminous flux.But the now widely used transparent bacterium bag of monochrome can not play the effect of shading.The present invention is by bacterium bag
Preparing raw material in add titanium dioxide, reflective agent, can reflect, mask substantial amounts of ultraviolet light and infrared light, so as to effectively keep away
Exempt from strong illumination to mycelia, the injury of ear bud；And the light conversion agent added can not be reflected fraction, the infrared light that masks,
Ultraviolet light is converted into the light blue light that may advantageously facilitate bacteria stick growth, micro- feux rouges etc., plays the effect of luminous energy fertilizer.
Using the method for bacterium bag open air cultivating black fungus, comprise the following steps：
1）Inoculation：After compost is sterilized, strain is uniformly admixed；Inoculum concentration is to be inoculated with 5 ~ 10g strains per 200g composts；
2）Bacterium germination culture：By the bacteria stick after inoculation in bacterium germination culture 15 ~ 20 days in dark culturing room；
3）Outdoor cultivation：The bacteria stick for covering with mycelia is taken out out of culturing room, punched in the non-transparent portion of bacterium bag, it is general long
24cm bacteria stick can perforate 200 or so, its aperture is no more than 2mm, then in outdoor placement carry out produce agaric culture；
4）Harvesting：Illustrate auricle maturation when white spore occurs in the black fungus piece outside of belly, can be harvested, can typically harvest 5 ~ 7
Stubble agaric.
Bacterium germination culture need to be carried out under dark condition, it is seen that light is more few better.Bacterium bag of the present invention due to exhausted big position shading,
Light is shielded, thus is advantageous to the bacterium germination growth of mycelia, its bacterium germination time cultivated can be made to shorten 5 ~ 7 days（Generally 35-40 days）,
And the probability of miscellaneous bacteria infection can be reduced.
Bacterium bag of the present invention with bacteria stick due to that with good pliability and elasticity, can be brought into close contact, so as to hinder
Only the growth of wall mushroom pushes up bag and produces a bag rod separation, and then prevents the generation of " steaming Saunas " phenomenon；And in outdoor cultivation, pass through
Punched in the non-transparent portion of bacterium bag, mycelia only in tapping sprouting, quick-fried flower bud, is oriented produce agaric using artificial photoinduction.Cause
This, combines specific application method using bacterium bag of the present invention, can greatly improve the survival rate of bacteria stick and the biological transformation ratio of compost,
And the profile of black fungus, quality are lifted, yield is improved, realizes increasing both production and income.
Step 1）In compost used raw material composition be calculated as by weight：Broken 25 parts of corncob, wood fragments consider 8 parts, wheat bran 8 to be worth doing
Part, 5 parts of phoenix tree leaf powder, 4 parts of sucrose, 2 parts of gypsum, 2 parts of soapberry pericarp, 1 part of haydite；The phoenix tree leaf powder is to wash phoenix tree leaf
It is grilled to dry after net, then be made through crushing.
Step 3）Middle bacteria stick is 3 ~ 5cm in the line-spacing that open air is put, and spacing is 2 ~ 3cm；Wherein, bacterium bag hyalomere court should be made
Eastwards, to promote mycelia from tapping fruiting.
In traditional outdoor cultivation of black fungus, if putting bacterium bag is intensive, under light illumination, bacterium can be made because of atmosphere draught-free
Rod internal temperature is too high, occurs " setting sun disease ".Therefore, in cultivating out of doors, the distance between bacterium bag need to be controlled in more than 10cm.And
Bacterium bag of the present invention can effectively stop light due to only partially transparent, be heated up so as to effectively avoid heat absorption inside bacteria stick, because
This, using the outdoor cultivation of bacterium bag of the present invention progress, the distance between its bacterium bag is positively retained at 3 ~ 5cm, intensive so as to be advantageously implemented
Cultivation and high yield.
In a word, the invention provides one kind to have good high temperature resistant, black wood that is high pressure resistant and possessing " soft, tough, bullet " characteristic
Ear open air cultivating bacteria bag, it has good out pocket and the affinity with bacteria stick, so as to prevent a bag rod separation, avoids mould
Change and the generation of rotten rod；Meanwhile the outdoor cultivating bacteria bag is in addition to the transmittance section for observing the internal mycelial growth situation of bag, mainly
For non-transparent portion, its can effective shielded from sunlight, avoid bacterium bag from causing bacteria stick temperature to raise because of long-time strong illumination, damage bacterium
Silk, in conjunction with suitable arrangement method and application method, each stage that can be grown in black fungus, reflected by light, light shield,
The multiple actions such as light conversion, light directional induction, reach the purpose for promoting mycelial growth, thalline to be formed, to shorten growth cycle, carry
High efficiency, and effectively prevent the generation of wall mushroom, setting sun disease.
Brief description of the drawings
Fig. 1 is the structural representation of black fungus open air of the present invention cultivating bacteria bag.
Fig. 2 is the view after the inoculation of black fungus open air of the present invention cultivating bacteria bag（Upper end sack ties up closing）.
Fig. 3 is to use schematic diagram in cultivating bacteria bag bacterium germination incubation in black fungus open air of the present invention.
Fig. 4 is to use schematic diagram in the cultivation of black fungus open air cultivating bacteria bag open air of the present invention.
Embodiment
As shown in figure 1, a kind of black fungus open air cultivating bacteria bag, including one bag of body 10, this bag of middle side part of body one, which has, to be used for
The hyalomere 110 of the internal mycelial growth situation of bag is observed, remainder is non-transparent 120.
For bag body hyalomere 110 in strip through bag body head and the tail, its width is 1 ~ 2cm.
The non-transparent part 120 of bag body can be white, grey or black, preferably grey.
Bag body one end closing.
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Embodiment 1
A kind of preparation method of black fungus open air cultivating bacteria bag, it is by high density polyethylene (HDPE), polyolefin elastomer, titanium dioxide, light
Stabilizer, light conversion agent, antioxidant, dispersant, reflective agent, coupling agent, toughener are mixed to prepare group through homogenizer in proportion
Divide A, by high density polyethylene (HDPE), very low density polyethylene and polyolefin elastomer after homogenizer is mixed to prepare component B；Will
Component A, B is respectively through dual-screw pelletizer extruding pelletization, then using the blowout shaping of double-colored inflation film machine, then through automatic otch
Machine seals, and produces.
Wherein preparing the raw materials used percetage by weight in the non-transparent part of bacterium bag is：High density polyethylene (HDPE)（HPDE）50%th, gather
Olefin elastomer（POE）20%th, titanium dioxide 15%, light stabilizer 2%, light conversion agent 1%, antioxidant 1.5%, dispersant 3.5%, reflective agent
2%th, coupling agent 1.5%, toughener 3.5%；
The percetage by weight of primary raw material is used in preparation bacterium bag hyalomere：High density polyethylene (HDPE)（HPDE）75%th, extra-low density gathers
Ethene（LLD）15%th, polyolefin elastomer（POE）10%；To make raw material stable, it also needs to add a small amount of antioxidant in preparing
Table 1 is the measuring mechanical property data of bacterium bag of the present invention.
The measuring mechanical property of table 1
From table 1, bacterium bag of the present invention meets relevant criterion, has good mechanical performance.After testing, the present invention is made simultaneously
Standby cultivating bacteria bag can bear 125 DEG C thermophilic digestion, it is indeformable, do not soften, and with good weather-proof, weathering resistance, anti-embrittlement
Performance.
Embodiment 2
Using the method for bacterium bag open air cultivating black fungus prepared by embodiment 1, comprise the following steps：
1）Inoculation：Load bag body 10 after compost is mixed thoroughly, after sealed sterilizing, strain is uniformly admixed in compost and connect
Kind, inoculum concentration is to be inoculated with 5 ~ 10g strains per 200g composts；By another end closure after the completion of inoculation（As shown in Figure 2）；
2）Bacterium germination culture：By the bacteria stick after inoculation in dark culturing room, bacterium germination culture 15 ~ 20 days on culturing rack 101,（Such as Fig. 3
It is shown）, a bag body hyalomere is placed inwardly；As long as from being carried out in incubation from bacterium bag hyalomere, you can know mycelia
Growing state；
3）Outdoor cultivation：The bacteria stick for covering with mycelia is taken out out of culturing room, carries out punching 130 in the non-transparent portion of bacterium bag, typically
Long 24cm bacteria stick can perforate 200 or so, its aperture is no more than 2mm（As shown in Figure 4）, then carry out produce agaric in outdoor placement
Culture；
4）Harvesting：Illustrate auricle maturation when white spore occurs in the black fungus piece outside of belly, harvested, can typically harvest 5 ~ 7 batches
Agaric.
Step 1）In compost used raw material composition be by weight：Broken 25 parts of corncob, wood fragments consider 8 parts, wheat bran 8 to be worth doing
Part, 5 parts of phoenix tree leaf powder, 4 parts of sucrose, 2 parts of gypsum, 2 parts of soapberry pericarp, 1 part of haydite；The phoenix tree leaf powder is to wash phoenix tree leaf
It is grilled to dry after net, then crushed and be made.
Step 3）Middle bacteria stick is 3 ~ 5cm in the line-spacing that open air is put, and spacing is 2 ~ 3cm；Wherein, bacterium bag hyalomere court should be made
Eastwards, to promote mycelia from tapping fruiting.
After testing,, can more traditional list using bacteria stick internal temperature made of bacterium bag of the present invention under the irradiation of strong sunshine
The internal temperature that bacteria stick is made in the transparent bacterium bag of color is low 5 ~ 10 DEG C, it was demonstrated that it has the effect of good light-shading control heat.
Embodiment 3
Cultivation contrast test is carried out using different disposal, result of the test is as shown in table 2（Wherein, monochromatic transparent bacterium bag is planted out of doors
A bag mode is cut using routine substitute punching during training）.
Cellar culture material formula used is（By weight）：Broken 30 parts of corncob, 10 parts of wood fragments bits, 8 parts of wheat bran, sucrose
4 parts, 2 parts of gypsum, 1 part of lime.
The experiment in cultivation result of the different disposal of table 2
From table 2, compared with monochromatic transparent bacterium bag, cultivation of auricularia auricula is carried out using bacterium bag of the present invention, can substantially shorten growth
In the cycle, production efficiency is improved, and can effectively prevent the generation of wall mushroom, setting sun disease；Compared with cellar culture material, trained using the present invention
Nutriment is cultivated, and has the function that to improve yield.Therefore, invention bacterium bag is used with reference to its compost, had
The effect of obvious good quality and high output.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (10)
1. a kind of black fungus open air cultivating bacteria bag, including one bag of body, it is characterised in that：Bag body one middle side part, which has, to be used to observe bag
The hyalomere of internal mycelial growth situation, remainder are non-transparent.
2. black fungus open air cultivating bacteria bag according to claim 1, it is characterised in that：Bag body hyalomere runs through bag body in strip
From beginning to end, its width is 1 ~ 2cm.
3. black fungus open air cultivating bacteria bag according to claim 1, it is characterised in that：The non-transparent part of bag body is white, ash
Color or black.
4. black fungus open air cultivating bacteria bag according to claim 1, it is characterised in that：Bag body one end closing.
A kind of 5. preparation method of bacterium bag as claimed in claim 1, it is characterised in that：The non-transparent part of bacterium bag is by following weight hundred
The raw material of fraction is made：High density polyethylene (HDPE) 50%, polyolefin elastomer 20%, titanium dioxide 15%, light stabilizer 2%, light conversion agent 1%,
Antioxidant 1.5%, dispersant 3.5%, reflective agent 2%, coupling agent 1.5%, toughener 3.5%；
The transparent part of bacterium bag is mainly made up of the raw material of following percetage by weight：High density polyethylene (HDPE) 75%, very low density polyethylene
15%th, polyolefin elastomer 10%.
6. the preparation method of bacterium bag according to claim 5, it is characterised in that：By high density polyethylene (HDPE), polyolefin elastomer,
Titanium dioxide, light stabilizer, light conversion agent, antioxidant, dispersant, reflective agent, coupling agent, toughener mix through homogenizer in proportion
Close and component A is made, high density polyethylene (HDPE), very low density polyethylene and polyolefin elastomer are mixed to prepare group through homogenizer
After dividing B；By component A, B respectively through dual-screw pelletizer extruding pelletization, then using the blowout shaping of double-colored inflation film machine, then pass through
Automatic notch cutter seals, and produces.
A kind of 7. method using bacterium bag open air cultivating black fungus described in claim 1, it is characterised in that：Comprise the following steps：
1）Inoculation：By compost it is sterilized after, uniformly admix strain；
2）Bacterium germination culture：By the bacteria stick after inoculation in bacterium germination culture 15 ~ 20 days in dark culturing room；
3）Outdoor cultivation：The bacteria stick for covering with mycelia is taken out out of culturing room, punched in the non-transparent portion of bacterium bag, its aperture is not
More than 2mm, then produce agaric culture is carried out in outdoor placement；
4）Harvesting：Illustrate auricle maturation when white spore occurs in the black fungus piece outside of belly, harvested.
8. the method for outdoor cultivating black fungus according to claim 7, it is characterised in that：Step 1）In compost used original
Material composition is calculated as by weight：Broken 25 parts of corncob, 8 parts of wood fragments bits, 8 parts of wheat bran, 5 parts of phoenix tree leaf powder, 4 parts of sucrose, gypsum 2
Part, 2 parts of soapberry pericarp, 1 part of haydite.
9. the method for outdoor cultivating black fungus according to claim 7, it is characterised in that：Step 1）In connect per 200g composts
Kind 5-10g strains.
10. the method for outdoor cultivating black fungus according to claim 7, it is characterised in that：Step 3）Middle bacteria stick is in outdoor pendulum
The line-spacing put is 3 ~ 5cm, and spacing is 2 ~ 3cm；Wherein, bacterium bag hyalomere should be made towards in the east.
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| CN109937803A (en) * | 2019-04-28 | 2019-06-28 | 蔡世荣 | A method of hamlessizing technology black fungus is carried out using bacteria stick stem |
| CN110741883A (en) * | 2019-11-05 | 2020-02-04 | 大连富森智能科技有限公司 | Edible mushroom cultivation bag |
| CN110881364A (en) * | 2019-12-30 | 2020-03-17 | 郴州市芝草农业科技开发有限公司 | Edible mushroom planting bag |
| CN111512886A (en) * | 2020-04-29 | 2020-08-11 | 黑龙江黑臻生物科技有限公司 | Bilateral fruiting method of pleurotus eryngii |
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| CN109937803B (en) * | 2019-04-28 | 2023-11-24 | 蔡世荣 | Method for harmless production of black fungus by using fungus stick core column |
| CN110741883A (en) * | 2019-11-05 | 2020-02-04 | 大连富森智能科技有限公司 | Edible mushroom cultivation bag |
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