CN107619645A - A kind of method that dopamine enhancing starch-based adhesive is prepared based on holoenzyme method - Google Patents
A kind of method that dopamine enhancing starch-based adhesive is prepared based on holoenzyme method Download PDFInfo
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- CN107619645A CN107619645A CN201711057701.9A CN201711057701A CN107619645A CN 107619645 A CN107619645 A CN 107619645A CN 201711057701 A CN201711057701 A CN 201711057701A CN 107619645 A CN107619645 A CN 107619645A
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- laccase
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Abstract
The invention discloses a kind of method that dopamine enhancing starch-based adhesive is prepared based on holoenzyme method, starch is set to produce aldehyde radical using TEMPO/ laccase systems, it forms firm covalent bond with dopamine by aldimine condensation, at the same time more Buckies enzymatic oxidation under laccase effect so as to produce high bond strength starch-based adhesive, improves the performance and application of amylum adhesive.
Description
Technical field
The present invention relates to a kind of method that dopamine enhancing starch-based adhesive is prepared based on holoenzyme method, and in particular to a kind of
Amylum adhesive of high-adhesion energy and application thereof, belongs to technical field of macromolecules.
Background technology
The 1950s, natural glue are gradually synthesized adhesive and substituted, but the day of petroleum resources at present
Gradually exhausted and environmentally friendly cry is increasingly surging, develops and utilizes natural reproducible resource and is increasingly weighed to prepare adhesive
Depending on.Increasing natural material physics and chemical denaturing methods are used for the synthesis performance for improving natural glue, especially
It is how to improve the adhesion strength of starch-based adhesive.
Mucus of the mussel secreted by it can be almost adsorbed on the surface of any base material, show superpower adhesion energy
Power.Recent studies indicate that the Adhering capacity that mussel is superpower, this has benefited from containing substantial amounts of dihydroxy phenylpropyl alcohol ammonia in its mucus
Catechol structure in acid.According to this characteristic, starch polymer chain is modified to obtain by catechol group bond energy
Power is excellent, high-strength binding material.
TEMPO and amboceptor of its derivative as Laccase Catalyzed system are widely used, and TEMPO/ laccases can incite somebody to action
Hydroxyl on grape carbohydrates and their derivative is oxidized to aldehyde radical and carboxyl.Therefore, make using the method processing starch of enzymatic oxidation
It produces aldehyde radical or carboxyl, and aldimine condensation reaction then occurs with dopamine, dopamine is covalently attached on starch molecular chain,
Recycle laccase that more Buckies (DOPA) on starch molecular chain are carried out with the high-strength binding material of enzymatic oxidation production ecological, environmental protective,
It can be widely applied to the fields such as weaving, papermaking, house ornamentation.
The content of the invention
The technical problems to be solved by the invention are to provide the side that a kind of holoenzyme prepares dopamine enhancing starch-based adhesive
Method, starch is set to produce aldehyde radical by TEMPO/ laccase systems, it forms firm covalent bond with dopamine by aldimine condensation,
At the same time more Buckies enzymatic oxidation under laccase effect so as to produce high bond strength starch-based adhesive, improves starch adhesion
The performance and application of agent, concrete technology are as follows with step：
Weigh 30g starch, with 0.1M, NaAc-HAc buffer solutions that pH is 5.0 will starch it is dense be adjusted to 15%, add laccase
(2mL, 1000UmL-1) and TEMPO (2%, to starch on dry basis), blowing air, reacted under conditions of 30 DEG C, it is more that 10g is added after 2h
After bar amine stirring 1h, it is heated to 95 DEG C of gelatinization 10min and obtains dopamine enhancing starch-based adhesive.
The present invention is introduced using TEMPO/ laccase system selective oxidation starch primary hydroxyl generation aldehyde radical by aldimine condensation
DOPA groups, while Laccase Catalyzed oxidation DOPA groups prepare dopamine enhancing starch-based adhesive, this method not only can be significantly
The adhesive property of starch-based adhesive is lifted, with extracting adhesion protein liquid directly from mussel or manually being closed by method of gene recombination
Compared into the viscose albumen containing DOPA with polypeptide, cost is greatly reduced.
Embodiment
Starch-based adhesive is prepared using holoenzyme method, specific embodiment is as follows：
Embodiment 1
Weigh 30g cornstarch, with 0.1M, NaAc-HAc buffer solutions that pH is 5.0 will starch it is dense be adjusted to 15%, add laccase
(2mL, 1000UmL-1) and TEMPO (2%, to starch on dry basis), blowing air, reacted under conditions of 30 DEG C, it is more that 10g is added after 2h
After bar amine stirring 1h, it is heated to 95 DEG C of gelatinization 10min and obtains dopamine enhancing starch-based adhesive.
Embodiment 2
Weigh 30g farinas, with 0.1M, phosphate buffer that pH is 6.0 will starch it is dense be adjusted to 25%, add laccase
(10mL, 1000UmL-1) and TEMPO (8%, to starch on dry basis), blowing air, reacted under conditions of 40 DEG C, 15g is added after 8h
After dopamine stirring 2h, it is heated to 95 DEG C of gelatinization 10min and obtains dopamine enhancing starch-based adhesive.
Embodiment 3
Weigh 30g tapiocas, with 0.1M, citrate buffer solution that pH is 4.8 will starch it is dense be adjusted to 10%, add laccase
(1mL, 1000UmL-1) and TEMPO (1%, to starch on dry basis), blowing air, reacted under conditions of 50 DEG C, it is more that 5g is added after 20h
After bar amine stirring 1h, it is heated to 95 DEG C of gelatinization 10min and obtains dopamine enhancing starch-based adhesive.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, it can all do various change and modification, therefore the protection model of the present invention
Enclose being defined of being defined by claims.
Claims (8)
1. a kind of method that dopamine enhancing starch-based adhesive is prepared based on holoenzyme method, concrete technology are as follows with step：
Weigh 30g starch, with 0.1M, NaAc-HAc buffer solutions that pH is 5.0 will starch it is dense be adjusted to 15%, add laccase (2mL,
1000UmL-1) and TEMPO (2%, to starch on dry basis), blowing air, reacted under conditions of 30 DEG C, 10g dopamines are added after 2h
After stirring 1h, it is heated to 95 DEG C of gelatinization 10min and obtains dopamine enhancing starch-based adhesive.
2. according to the method for claim 1, it is characterised in that：The starch base is imitated when prepared by mussel adhesive and selects raw material
For cornstarch, wheaten starch, tapioca, farina and its corresponding converted starch, in addition, the object of the present invention removes
Outside its derivative of starch base, further relate to that there is the carragheen of adhesive property, guar gum, Arabic gum, konjac glucomannan, xanthans, knot
Cold glue.
3. according to the method for claim 1, it is characterised in that：Described cushioning liquid is that the buffering that enzyme activity can be made stable is molten
Liquid, such as phosphate buffer solution, citrate buffer solution, acetic acid sodium acetate.
4. according to the method for claim 1, it is characterised in that：Add more than 1% that laccase amount is starch on dry basis dosage.
5. according to the method for claim 1, it is characterised in that：Add more than 5% that TEMPO amounts are starch on dry basis dosage.
6. according to the method for claim 1, it is characterised in that：Laccase/TEMPO oxidizing reaction temperatures are 10-60 DEG C.
7. according to the method for claim 1, it is characterised in that：Add more than 2% that dopamine amount is starch on dry basis.
8. according to the method for claim 1, it is characterised in that：Mixing time is not less than 5min after adding dopamine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711057701.9A CN107619645B (en) | 2017-11-01 | 2017-11-01 | Method for preparing dopamine-enhanced starch-based adhesive based on holoenzyme method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711057701.9A CN107619645B (en) | 2017-11-01 | 2017-11-01 | Method for preparing dopamine-enhanced starch-based adhesive based on holoenzyme method |
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| CN107619645A true CN107619645A (en) | 2018-01-23 |
| CN107619645B CN107619645B (en) | 2021-09-03 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109403089A (en) * | 2018-10-15 | 2019-03-01 | 安阳师范学院 | A kind of coating dye printing Environment-friendlyadhesive adhesive and its preparation method and application |
| CN111040667A (en) * | 2019-12-20 | 2020-04-21 | 湖南工业大学 | Dopamine-functionalized starch adhesive and preparation method thereof |
| CN111560226A (en) * | 2019-12-20 | 2020-08-21 | 湖南工业大学 | Starch adhesive and preparation method thereof |
| CN112409974A (en) * | 2020-11-19 | 2021-02-26 | 江南大学 | Amphiphilic bio-based degradable underwater adhesive and preparation method and application thereof |
| CN116607351A (en) * | 2023-05-22 | 2023-08-18 | 海南椰国食品有限公司 | Nanometer bacterial cellulose hydrophobic oil-proof paper and preparation method thereof |
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| US20170260429A1 (en) * | 2010-11-15 | 2017-09-14 | Agrana Staerke Gmbh | Starch-based glue composition |
| CN103554283A (en) * | 2013-10-10 | 2014-02-05 | 西北大学 | Functional starch containing dopamine and polyethylene glycol, and preparation method and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109403089A (en) * | 2018-10-15 | 2019-03-01 | 安阳师范学院 | A kind of coating dye printing Environment-friendlyadhesive adhesive and its preparation method and application |
| CN111040667A (en) * | 2019-12-20 | 2020-04-21 | 湖南工业大学 | Dopamine-functionalized starch adhesive and preparation method thereof |
| CN111560226A (en) * | 2019-12-20 | 2020-08-21 | 湖南工业大学 | Starch adhesive and preparation method thereof |
| CN112409974A (en) * | 2020-11-19 | 2021-02-26 | 江南大学 | Amphiphilic bio-based degradable underwater adhesive and preparation method and application thereof |
| CN112409974B (en) * | 2020-11-19 | 2021-12-21 | 江南大学 | Amphiphilic bio-based degradable underwater adhesive and preparation method and application thereof |
| CN116607351A (en) * | 2023-05-22 | 2023-08-18 | 海南椰国食品有限公司 | Nanometer bacterial cellulose hydrophobic oil-proof paper and preparation method thereof |
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| CN107619645B (en) | 2021-09-03 |
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