CN107616354A

CN107616354A - Increase the preparation method of the shallow sea fish feed additive of Meat Tenderness

Info

Publication number: CN107616354A
Application number: CN201710732780.2A
Authority: CN
Inventors: 陈义娜
Original assignee: Pujiang County Cooperative Animal Feed Technology Development Co Ltd
Current assignee: Pujiang County Cooperative Animal Feed Technology Development Co Ltd
Priority date: 2017-08-24
Filing date: 2017-08-24
Publication date: 2018-01-23

Abstract

The invention discloses a kind of preparation method for the shallow sea fish feed additive for increasing Meat Tenderness, including：Extract fish guts oil, fish oil modification, the preparation of Chinese medicine preparation, complex additive.Have the beneficial effect that：The hydrogen of the centre position carbon-carbon double bond of saturated fatty acid can be seized generation water by enzymatic reaction by oxygen, so that chain saturated fatty acids lose hydrogen and are converted into corresponding unrighted acid, make in fish guts oil rich in polyunsaturated fatty acids such as DHA, EPA, it is especially enriched in unrighted acid of taking advantage of a situation, the lipid structure that can be effectively improved in the fish body of shallow sea, strengthen the elasticity and tenderness of the flesh of fish；Contain the nutritional ingredients such as abundant traditional Chinese medicine ingredients, phosphatide, vitamin, nucleotides, complex enzyme in additive, coordinate phagostimulant and antioxidant content, it is suitable as the feed addictive of shallow sea fish, after being eaten with feed formulation, stress ability, immunocompetence, the disease-resistant antibacterial ability of fish can be strengthened, promote it to digest and assimilate nutriment, accelerate to grow.

Description

Increase the preparation method of the shallow sea fish feed additive of Meat Tenderness

Technical field

The invention belongs to fish feed additive field, more particularly to a kind of shallow sea fish feed additive for increasing Meat Tenderness Preparation method.

Technical background

Shallow sea fish refers generally to move in the fish close within 200 meters of depth on sea.Shallow sea fish species are various, often The ratio seen is if any butterfish, yellow croaker, hairtail, grouper etc..In recent years, because wild shallow sea fish number falls sharply, have at present very Shallow sea fish before more successfully realize cultivation.So many common shallow sea fish on the market at present, in the known integrated circuit it is a fact that derive from Cultivation.Representative therein is exactly yellow croaker, how precious fish, grouper, golden pomfret etc..It is each in marine depth often more than 10 meters The area of square centimeter just has one kilogram of pressure, calculates, in 1000 meters of place of the depth of water, pressure every square centimeter is just More than 100 kilograms can be up to, so the pressure ratio shallow sea fish suffered by deep-sea fish length are much bigger.So typically, deep-sea The meat of fish can more consolidation some, unlike shallow sea flesh of fish matter taste it is so fresh and tender and delicious.

The flesh of fish belongs to lean meat species, and 100 grams of flesh of fish institutes are fatty less than 2 grams, and 100 grams of sausages are fat in 10 grams.I.e. Just most greasy Norway salmon, heat contained by it also half fewer than pork chop.The important sources of the flesh of fish or protein.The flesh of fish holds Easily it is absorbed by the body, 100 grams of flesh of fish ensure the half of human body protein required daily.The flesh of fish also supplies the dimension required for human body Raw plain A, D, E etc..Also contain a variety of aliphatic acid in the flesh of fish, this material can prevent blood viscosity from increasing, can effectively prevent heart The generation of disease, and the strong brain of energy and the retina of nerve fiber and eyes, for pregnant woman and baby, these aliphatic acid are more It is indispensable.The meat of the flesh of fish is improved, the quality for improving meat concerns the economic value and edibility of fish.

Feed additive technology on increasing meat quality of fish has many methods, and as applied, public number is CN to prior art 102845647 B Chinese invention patent, disclose a kind of Chinese herbal feed additive for improving carp meat and its preparation side Method, the volatile oil that the inventive method distills the bark of eucommia, dandelion, the root of Dahurain angelica, Chinese yam, the concentrate of chrysanthemum and chrysanthemum collection mix i.e. Chinese herbal feed additive, have and prepare the advantages of simple, easy to use, safe nontoxic, but it is only with five The extract component of herbal medicine can only also improve the disease-resistant antibacterial characteristics of carp, improve its immunity, Wu Fagai as feed addictive It is apt to it to digest and assimilate, it is impossible to change its fat, quality of protein, therefore improve the degree of carp meat than relatively limited.

The content of the invention

The purpose of this method is to provide a kind of preparation method for the shallow sea fish feed additive for increasing Meat Tenderness, prepared Method is simple to operation, and feed addictive nutritional ingredient is balanced, can be effectively improved the lipid structure of shallow sea fish, strengthen the flesh of fish Elasticity and tenderness.

The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：Increase the shallow sea fish of Meat Tenderness The preparation method of feed addictive, including：Extract fish guts oil, fish oil modification, the preparation of Chinese medicine preparation, complex additive, tool Body comprises the following steps：

Extract fish guts oil：Fresh bighead internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner Slurry, fish guts homogenate is weighed into container, adds the distilled water of equivalent part, stir lower warming-in-water to 33-36 DEG C, regulation pH is arrived 8-9, continue stirring and be warming up to 55-57 DEG C, be incubated 35-45 minutes, add salt after fish guts weight 8-10% potassium chloride stirring and dissolving Centrifuged while hot after analysis 30-40 minutes, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, is done for further modification Prepare；This method fish oil yield is high, simple to operate, and waste water, waste residue can be made into the utilization of green high-efficient fertilizer total integration, can be with Raw material as extraction nucleic acid is further high-valued, has reached the purpose of waste's reclamation；

Fish oil is modified：Weigh crude fish oil 150-180 parts, vitamin E 0.05-0.08 parts are put in closed container and are well mixed, general Reaction vessel is placed in Ultrasound Instrument, is heated to 68-70 DEG C and is kept, and is continually fed into oxygen 10-15 minutes, is then added desaturation Enzyme 0.5-0.6 parts, reduced Coenzyme II 0.1-0.15 parts, functional polypeptide 0.06-0.10 parts, it is 300-350W, frequency in power The praetersonic effect lower reaction 3-5 minutes that rate is 45-60KHz；Siphon enzymatic fish oil and with hot water wash 2-3 times, is centrifuged while hot, Supernatant liquid is taken, modified fish guts oil is drying to obtain with vacuum drying chamber；The amino acid sequence of functional polypeptide is： SCASRCKSRCRARRCGYYVSVAAFYRGRCYCRCLRC；Enzymatic reaction can be by the centre position carbon-carbon double bond of saturated fatty acid Hydrogen generation water is seized by oxygen so that chain saturated fatty acids lose hydrogen and are converted into corresponding unrighted acid, make in fish Rich in polyunsaturated fatty acids such as DHA, EPA in dirty oil, the lipid structure in fish body can be improved after being ingested by shallow sea fish, increased The elasticity and tenderness of meat；Functional polypeptide can have an impact to the reactant of enzymatic reaction and the space structure of product, increase Add the accounting of cis unsaturated fatty acid in product, the content of trans unrighted acid in product is reduced, so as to strengthen insatiable hunger With the nutritive value of aliphatic acid, it is set to be highly suitable as the fortification composition of fish body；

The preparation of Chinese medicine preparation：By fleece-flower root 5-8 parts, hawthorn 5-10 parts, malt 10-12 parts, Divine Comedy 2-3 parts, bighead atractylodes rhizome 3-5 parts, Radix Astragali 6-8 parts, mast 10-15 parts are crushed with pulverizer, cross 80-100 mesh sieves, compound Chinese medicinal preparation is made, weighs in 10-12 parts Medicine compound preparation, wrapped with degreasing cotton yarn, add 100-120 part water, it is boiled after immersion 1-2 hours, it is cooked by slow fire 30-40 points Clock, filtered with gauze, filter residue is decocted again, and filtrate is mixed, and low temperature is concentrated into aqueous 30-35%, and 3-4 DEG C of preservation is standby With；Chinese medicine preparation can strengthen the disease-resistant antibacterial ability of shallow sea fish, improve immunity of organisms, build up health；

Complex additive：Weigh broken wall yeast 0.2-0.5 parts, Chinese medicine preparation 3-5 parts, Florfenicol 0.02-0.05 parts, citric acid It is 3-6 parts, allicin 0.2-0.3 parts, Modified Fish Oil 20-25 parts, soybean lecithin 10-15 parts, vitamin premix 1-3 parts, multiple Synkaryon thuja acid 2-4 parts, complex enzyme formulation 0.3-0.6 parts, phagostimulant 1-2 parts, cuttlefish powder 5.0-6.0 parts, Choline Chloride 0.3-0.5 Part, taurine 1.0-1.5 parts crush after being well mixed, and cross 100-120 mesh sieves, and outsifting continues to crush until whole sieve, i.e., Obtain increasing the feed addictive of shallow sea meat quality of fish tenderness；Contain abundant traditional Chinese medicine ingredients, phosphatide, dimension life in additive The nutritional ingredients such as element, nucleotides, complex enzyme, coordinate phagostimulant and antioxidant content, be suitable as the feed addictive of shallow sea fish, After being eaten with feed formulation, stress ability, immunocompetence, the disease-resistant antibacterial ability of fish can be strengthened, promote it to nutriment Digest and assimilate, accelerate to grow；Containing abundant unrighted acid in feed addictive, can change after fish body is entered The fatty acid structure of body, strengthen the elasticity and tenderness of the flesh of fish, therefore there is higher nutritive value and economic value, be a kind of Efficiently, the feed addictive of safe increase shallow sea meat quality of fish tenderness.

Preferably, vitamin premix used is VA or VB₁Or VB₂Or VB₆Or VB₁₂Or VC or VD or VE or VK or Calcium pantothenate or nicotinic acid or folic acid or biotin or inositol or phosphoric acid is cruel or at least five kinds of mixture of microcrystalline cellulose；Vitamin Premix can strengthen the antibacterial resistance against diseases of close shrimp, improve immunity, copingability, reduce the death rate.

Preferably, compound nucleotide used is adenosine acid disodium and guanosine acid disodium and cytimidine The mixture of disodium 5 '-ribonucleotide and uridine acid disodium and inosinic acid disodium；Nucleotides can be used as protein Kinases strengthens metabolic enzyme activity, the synthesis of inducing hormone or enzyme, promotes the synthesis of juvenile fish vivo protein, so that increase body Weight, promote growth.

Preferably, complex enzyme formulation used be protease and amylase with cellulase and carbohydrase and lipase with The mixture of pectase；Complex enzyme formulation can improve grouper and nutritional ingredient is digested and assimilated, and lift nutrition balance degree, Improve the quality of the flesh of fish.

Preferably, phagostimulant used is glycine betaine or neopentanoic acid thetin or bark of eucommia powder or glycine or dusty yeast Or alanine or 5- inosinyl phosphate inosines or dried orange peel or brewex's grains or dried silkworm chrysalis meal；Composite feeding promoting agent can strengthen the palatability of feed, from And juvenile fish is promoted to feed.

Compared with prior art, the advantage of the invention is that：1）Enzymatic reaction can be by the centre position carbon of saturated fatty acid The hydrogen of carbon double bond seizes generation water by oxygen, so that chain saturated fatty acids lose hydrogen and are converted into corresponding unrighted acid, So that unrighted acid of taking advantage of a situation is especially enriched in rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil, can be effective Improve the lipid structure in the fish body of shallow sea, strengthen the elasticity and tenderness of the flesh of fish；2）Contain abundant traditional Chinese medicine ingredients, phosphorus in additive The nutritional ingredients such as fat, vitamin, nucleotides, complex enzyme, coordinate phagostimulant and antioxidant content, be suitable as the feed of shallow sea fish Additive, after being eaten with feed formulation, stress ability, immunocompetence, the disease-resistant antibacterial ability of fish can be strengthened, promote it to battalion Digesting and assimilating for material is supported, accelerates to grow.

Embodiment

The present invention program is described further below by embodiment：

Embodiment 1：

Increase the preparation method of the shallow sea fish feed additive of Meat Tenderness, comprise the following steps：

1）Take fresh bighead internal organ homogenate to add the distilled water of equivalent part into container, stir lower warming-in-water to 33 DEG C, regulation PH to 8, continue stirring and be warming up to 55 DEG C, be incubated 35 minutes, add after fish guts weigh 8% potassium chloride stirring and dissolving and saltout 30 points Centrifuged while hot after clock, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, is prepared for further modification；

2）Weigh 150 parts of crude fish oil, 0.05 part of vitamin E be put in closed container be well mixed, reaction vessel is placed in ultrasound In instrument, it is heated to 68 DEG C and keeps, be continually fed into oxygen 10 minutes, then adds 0.5 part of desaturase, reduced Coenzyme II 0.1 part, functional polypeptide 0.06- parts, reacted 3 minutes under the praetersonic effect that power is 300W, frequency is 45KHz；Rainbow Inhale enzymatic fish oil and with hot water wash 2 times, centrifuge while hot, take supernatant liquid, it is oily to be drying to obtain modified fish guts with vacuum drying chamber； The hydrogen of the centre position carbon-carbon double bond of saturated fatty acid can be seized generation water by enzymatic reaction by oxygen, so that long-chain saturated fat Acid loses hydrogen and is converted into corresponding unrighted acid, makes rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil, shallow Ocean fish can improve the lipid structure in fish body after ingesting, increase the elasticity and tenderness of meat；

3）By 5 parts of the fleece-flower root, 5 parts of hawthorn, 10 parts of malt, 2 parts of Divine Comedy, 3 parts of the bighead atractylodes rhizome, 6 parts of the Radix Astragali, 10 parts of mast pulverizer powder It is broken, 80 mesh sieves are crossed, compound Chinese medicinal preparation is made, weighs 10 parts of compound Chinese medicinal preparations, is wrapped with degreasing cotton yarn, add 100 parts of water, It is boiled after soaking 1 hour, it is cooked by slow fire 30 minutes, is filtered with gauze, filter residue is decocted again, and filtrate is mixed, low temperature Aqueous 30% is concentrated into, 3 DEG C save backup；Chinese medicine preparation can strengthen the disease-resistant antibacterial ability of shallow sea fish, improve immunity of organism Power, build up health；

4）Weigh 0.2 part of broken wall yeast, 3 parts of Chinese medicine preparation, 0.02 part of Florfenicol, 3 parts of citric acid, 0.2 part of allicin, modification 20 parts of fish oil, 10 parts of soybean lecithin, 1 part of vitamin premix, 2 parts of compound nucleotide, 0.3 part of complex enzyme formulation, glycine betaine 0.5 part, 0.5 part of neopentanoic acid thetin, 5.0 parts of cuttlefish powder, 0.3 part of Choline Chloride, 1.0 parts of taurine it is well mixed after powder It is broken, 100 mesh sieves are crossed, outsifting continues to crush until all sieving, that is, the feed for obtaining increasing shallow sea meat quality of fish tenderness add Add agent；Containing nutritional ingredients such as abundant traditional Chinese medicine ingredients, phosphatide, vitamin, nucleotides, complex enzymes in additive, coordinate food calling Agent and antioxidant content, be suitable as the feed addictive of shallow sea fish, with feed formulation it is edible after, can strengthen fish stress energy Power, immunocompetence, disease-resistant antibacterial ability, promote it to digest and assimilate nutriment, accelerate to grow.

Embodiment 2：

1）Extract fish guts oil：Take fresh bighead internal organ homogenate to add the distilled water of equivalent part into container, stir lower water-bath liter Temperature adjusts pH to 9 to 36 DEG C, continues stirring and is warming up to 57 DEG C, is incubated 45 minutes, adds the potassium chloride stirring that fish guts weigh 10% Centrifuged while hot after being saltoutd after dissolving 40 minutes, separate to obtain crude fish oil；This method fish oil yield is high, and simple to operate, waste water, waste residue can The utilization of green high-efficient fertilizer total integration is made, it is further high-valued to be also used as extracting the raw material of nucleic acid, has reached discarded The purpose of thing comprehensive utilization；

2）Fish oil is modified：Weigh 180 parts of crude fish oil, 0.08 part of vitamin E be put in closed container be well mixed, by reaction vessel It is placed in Ultrasound Instrument, is heated to 70 DEG C and keeps, be continually fed into oxygen 15 minutes, then adds 0.6 part of desaturase, reduced form 0.15 part of codehydrogenase Ⅱ, 0.10 part of functional polypeptide, react 5 points under the praetersonic effect that power is 350W, frequency is 60KHz Clock；Siphon enzymatic fish oil and with hot water wash 3 times, centrifuges, takes supernatant liquid, be drying to obtain with vacuum drying chamber in modified fish while hot Dirty oil；The amino acid sequence of functional polypeptide is：SCASRCKSRCRARRCGYYVSVAAFYRGRCYCRCLRC；Functional polypeptide The reactant of enzymatic reaction and the space structure of product can be had an impact, cis unsaturated fatty acid accounts in increase product Than reducing the content of trans unrighted acid in product, so as to strengthen the nutritive value of unrighted acid, making it very suitable Cooperate the fortification composition for fish body；

3）The preparation of Chinese medicine preparation：By 8 parts of the fleece-flower root, 10 parts of hawthorn, 12 parts of malt, 3 parts of Divine Comedy, 5 parts of the bighead atractylodes rhizome, 8 parts of the Radix Astragali, mast 15 parts of son is crushed with pulverizer, crosses 100 mesh sieves, compound Chinese medicinal preparation is made, weighs 12 parts of compound Chinese medicinal preparations, with degreasing cotton yarn Wrap, add 120 parts of water, it is boiled after soaking 2 hours, it is cooked by slow fire 40 minutes, is filtered with gauze, filter residue is decocted again Boil, filtrate is mixed, low temperature is concentrated into aqueous 35%, and 4 DEG C save backup；Chinese medicine preparation can strengthen the disease-resistant antibacterial of shallow sea fish Ability, immunity of organisms is improved, is built up health；

4）Complex additive：Weigh 0.5 part of broken wall yeast, 5 parts of Chinese medicine preparation, 0.05 part of Florfenicol, 6 parts of citric acid, garlic 0.3 part of element, 25 parts of Modified Fish Oil, 15 parts of soybean lecithin, 3 parts of vitamin premix, 4 parts of compound nucleotide, complex enzyme formulation 0.6 part, 0.5 part of bark of eucommia powder, 0.5 part of alanine, 1.0 parts of 5- inosinyl phosphate inosines, 6.0 parts of cuttlefish powder, 0.5 part of Choline Chloride, ox sulphur Crushed after 1.5 parts of acid is well mixed, cross 120 mesh sieves, outsifting continues to crush until all sievings, that is, obtain that shallow sea can be increased The feed addictive of meat quality of fish tenderness；Contain abundant traditional Chinese medicine ingredients, phosphatide, vitamin, nucleotides, complex enzyme etc. in additive Nutritional ingredient, coordinate phagostimulant and antioxidant content, be suitable as the feed addictive of shallow sea fish, after being eaten with feed formulation, Stress ability, immunocompetence, the disease-resistant antibacterial ability of fish can be strengthened, promote it to digest and assimilate nutriment, accelerate life Long development；Containing unrighted acid is enriched in feed addictive, the fatty acid structure of body can be changed after fish body is entered, Strengthen the elasticity and tenderness of the flesh of fish, therefore there is higher nutritive value and economic value, be that a kind of increase efficiently, safe is shallow The feed addictive of ocean fish Meat Tenderness.

Embodiment 3：

Increase the preparation method of the shallow sea fish feed additive of Meat Tenderness, including：Extract fish guts oil, fish oil modification, Chinese medicine The preparation of preparation, complex additive, specifically include following steps：

Extract fish guts oil：Fresh bighead internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner Slurry, fish guts homogenate is weighed into container, adds the distilled water of equivalent part, stir lower warming-in-water to 35 DEG C, regulation pH is arrived 8.5, continue stirring and be warming up to 56 DEG C, be incubated 40 minutes, add after fish guts weigh 8% potassium chloride stirring and dissolving and saltout 35 minutes Centrifuge while hot afterwards, separate to obtain crude fish oil；Potassium method extracts fish guts oil to remove impurity, is prepared for further modification；This method Fish oil yield is high, simple to operate, and waste water, waste residue can be made into the utilization of green high-efficient fertilizer total integration, is also used as extracting core The raw material of acid is further high-valued, has reached the purpose of waste's reclamation；

Fish oil is modified：Weigh 160 parts of crude fish oil, 0.06 part of vitamin E be put in closed container be well mixed, reaction vessel is put In Ultrasound Instrument, it is heated to 70 DEG C and keeps, be continually fed into oxygen 15 minutes, it is auxiliary then adds 0.6 part of desaturase, reduced form II 0.1 parts of enzyme, 0.08 part of functional polypeptide, reacted 5 minutes under the praetersonic effect that power is 340W, frequency is 50KHz； Siphon enzymatic fish oil and with hot water wash 3 times, centrifuges, takes supernatant liquid, modified fish guts are drying to obtain with vacuum drying chamber while hot Oil；The amino acid sequence of functional polypeptide is：SCASRCKSRCRARRCGYYVSVAAFYRGRCYCRCLRC；Enzymatic reaction can incite somebody to action The hydrogen of the centre position carbon-carbon double bond of saturated fatty acid seizes generation water by oxygen, so that chain saturated fatty acids lose hydrogen conversion For corresponding unrighted acid, make rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil, can after being ingested by shallow sea fish To improve the lipid structure in fish body, increase the elasticity and tenderness of meat；Functional polypeptide can be to the reactant of enzymatic reaction Had an impact with the space structure of product, increase the accounting of cis unsaturated fatty acid in product, reduce trans insatiable hunger in product With the content of aliphatic acid, so as to strengthen the nutritive value of unrighted acid, it is set to be highly suitable as the fortification of fish body Composition；

The preparation of Chinese medicine preparation：By 5 parts of the fleece-flower root, 10 parts of hawthorn, 10 parts of malt, 3 parts of Divine Comedy, 5 parts of the bighead atractylodes rhizome, 8 parts of the Radix Astragali, mast 10 parts are crushed with pulverizer, cross 90 mesh sieves, compound Chinese medicinal preparation is made, weighs 12 parts of compound Chinese medicinal preparations, with degreasing cotton yarn bag It is good, 120 parts of water are added, it is boiled after soaking 2 hours, it is cooked by slow fire 30 minutes, is filtered with gauze, filter residue is decocted again, Filtrate is mixed, low temperature is concentrated into aqueous 30%, and 4 DEG C save backup；Chinese medicine preparation can strengthen the disease-resistant antibacterial energy of shallow sea fish Power, immunity of organisms is improved, is built up health；

Complex additive：Weigh 0.5 part of broken wall yeast, 4 parts of Chinese medicine preparation, 0.04 part of Florfenicol, 5 parts of citric acid, allicin 0.3 part, 24 parts of Modified Fish Oil, 14 parts of soybean lecithin, 2 parts of vitamin premix, 3 parts of compound nucleotide, complex enzyme formulation 0.4 Crushed after part, 1 part of brewex's grains, 5.0 parts of cuttlefish powder, 0.4 part of Choline Chloride, 1.0 parts of taurine are well mixed, cross 120 mesh sieves, sieve Go out thing to continue to crush the feed addictive for up to all sievings, that is, obtaining increasing shallow sea meat quality of fish tenderness；Contain in additive There are the nutritional ingredients such as abundant traditional Chinese medicine ingredients, phosphatide, vitamin, nucleotides, complex enzyme, coordinate phagostimulant and antioxidant content, Be suitable as the feed addictive of shallow sea fish, with feed formulation it is edible after, the stress ability of fish, immunocompetence, anti-can be strengthened Sick antibacterial ability, promotes it to digest and assimilate nutriment, accelerates to grow；Contain abundant unsaturation in feed addictive Aliphatic acid, the fatty acid structure of body can be changed after fish body is entered, strengthen the elasticity and tenderness of the flesh of fish, therefore with higher Nutritive value and economic value, be it is a kind of efficiently, safety increase shallow sea meat quality of fish tenderness feed addictive.

Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.

Technical scheme is described in detail embodiment described above, it should be understood that it is described above only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should be included in the scope of the protection.

Sequence table

<110>Animal feed technological development Co., Ltd is full of by Pujiang County association

<120>Increase the preparation method of the shallow sea fish feed additive of Meat Tenderness

<130> 4

<160> 1

<170> SIPOSequenceListing 1.0

<210> 2

<211> 36

<212> PRT

<213>Artificial synthesized (Mytilus coruscus)

<400> 2

Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Gly

1 5 10 15

Tyr Tyr Val Ser Val Ala Ala Phe Tyr Arg Gly Arg Cys Tyr Cys Arg

20 25 30

Cys Leu Arg Cys

35

Claims

1. increase the preparation method of the shallow sea fish feed additive of Meat Tenderness, including：Extract fish guts oil, fish oil it is modified, in The preparation of medicine preparation, complex additive, it is characterised in that：The step of described complex additive is：Weigh Chinese medicine preparation, modification Crushed after fish oil, soybean lecithin, vitamin premix, compound nucleotide, complex enzyme formulation, phagostimulant are well mixed, mistake 100-120 mesh sieves, outsifting continues to crush until all sievings, that is, obtain increasing the feed addition of shallow sea meat quality of fish tenderness Agent.

2. the preparation method of the shallow sea fish feed additive of increase Meat Tenderness according to claim 1, it is characterised in that： Described extraction fish guts oil step is：Fresh bighead internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, even It is homogenized in pulp grinder, weighs fish guts homogenate into container, add the distilled water of equivalent part, stirs lower warming-in-water to 33-36 DEG C, PH to 8-9 is adjusted, continues stirring and is warming up to 55-57 DEG C, be incubated 35-45 minutes, adds fish guts weight 8-10% potassium chloride stirring Saltout after dissolving and centrifuge while hot after 30-40 minutes, separate to obtain crude fish oil.

3. the preparation method of the shallow sea fish feed additive of increase Meat Tenderness according to claim 1, it is characterised in that： Described fish oil modification procedure is：Weigh crude fish oil 150-180 parts, vitamin E 0.05-0.08 parts are put in closed container and mixed Uniformly, reaction vessel is placed in Ultrasound Instrument, is heated to 68-70 DEG C and keeps, be continually fed into oxygen 10-15 minutes, Ran Houjia Enter desaturase 0.5-0.6 parts, reduced Coenzyme II 0.1-0.15 parts, functional polypeptide 0.06-0.10 parts, be 300- in power 350W, the praetersonic that frequency is 45-60KHz effect lower reaction 3-5 minutes；Siphon enzymatic fish oil and with hot water wash 2-3 times, takes advantage of Heat centrifugation, takes supernatant liquid, and modified fish guts oil is drying to obtain with vacuum drying chamber.

4. the preparation method of the shallow sea fish feed additive of increase Meat Tenderness according to claim 3, it is characterised in that： The amino acid sequence of functional polypeptide in described fish oil modification procedure is： SCASRCKSRCRARRCGYYVSVAAFYRGRCYCRCLRC。

5. the preparation method of the shallow sea fish feed additive of increase Meat Tenderness according to claim 1, it is characterised in that： The preparation steps of described Chinese medicine preparation are：By fleece-flower root 5-8 parts, hawthorn 5-10 parts, malt 10-12 parts, Divine Comedy 2-3 parts, white Art 3-5 parts, Radix Astragali 6-8 parts, mast 10-15 parts are crushed with pulverizer, cross 80-100 mesh sieves, compound Chinese medicinal preparation is made, weighs 10-12 part compound Chinese medicinal preparations, are wrapped with degreasing cotton yarn, add 100-120 part water, boiled after immersion 1-2 hours, are cooked by slow fire 30-40 minutes, filtered with gauze, filter residue is decocted again, and filtrate is mixed, and low temperature is concentrated into aqueous 30-35%, 3-4 DEG C Save backup.

6. the preparation method of the shallow sea fish feed additive of increase Meat Tenderness according to claim 1, it is characterised in that： Described complex additive step is：Weigh broken wall yeast 0.2-0.5 parts, Chinese medicine preparation 3-5 parts, Florfenicol 0.02-0.05 Part, citric acid 3-6 parts, allicin 0.2-0.3 parts, Modified Fish Oil 20-25 parts, soybean lecithin 10-15 parts, vitamin premix 1-3 parts, compound nucleotide 2-4 parts, complex enzyme formulation 0.3-0.6 parts, phagostimulant 1-2 parts, cuttlefish powder 5.0-6.0 parts, chlorination courage Crushed after alkali 0.3-0.5 parts, taurine 1.0-1.5 parts are well mixed, cross 100-120 mesh sieves, outsifting continues to crush until complete Portion sieves, that is, obtains increasing the feed addictive of shallow sea meat quality of fish tenderness.

7. the preparation method of the shallow sea fish feed additive of increase Meat Tenderness according to claim 6, it is characterised in that： Vitamin premix in described complex additive step is VA or VB₁Or VB₂Or VB₆Or VB₁₂Or VC or VD or VE or VK or Calcium pantothenate or nicotinic acid or folic acid or biotin or inositol or phosphoric acid is cruel or at least five kinds of mixture of microcrystalline cellulose, complex nucleus Thuja acid is adenosine acid disodium and guanosine acid disodium and cytidine acid disodium and uridine acid disodium With the mixture of inosinic acid disodium, complex enzyme formulation is protease and amylase and cellulase and carbohydrase and fat The mixture of fat enzyme and pectase, phagostimulant be glycine betaine or neopentanoic acid thetin or bark of eucommia powder or glycine or dusty yeast or Alanine or 5- inosinyl phosphate inosines or dried orange peel or brewex's grains or dried silkworm chrysalis meal.