CN107607504B - Bioluminescence developing agent for developing biological material evidence trace on metal object and developing method - Google Patents
Bioluminescence developing agent for developing biological material evidence trace on metal object and developing method Download PDFInfo
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- CN107607504B CN107607504B CN201710785473.0A CN201710785473A CN107607504B CN 107607504 B CN107607504 B CN 107607504B CN 201710785473 A CN201710785473 A CN 201710785473A CN 107607504 B CN107607504 B CN 107607504B
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Abstract
The invention relates to a bioluminescence developing agent for developing biological material evidence traces on a metal object, which comprises the following raw materials in percentage by mass of 100 percent: 1-2% of indene diketone, 15-25% of ethyl acetate, 2-5% of glycerol, 20-25% of pure alcohol and 43-62% of petroleum ether. The invention can show biological material evidence traces on the metal object by adjusting the formula proportion of the biological fluorescence showing agent; in addition, the invention does not need to be dried at high temperature and control the humidity of the object and the environment, thereby simplifying the display aspect and avoiding the damage to the biological material evidence on the object.
Description
Technical Field
The invention relates to a bioluminescence visualization agent and a visualization method for visualizing a biological evidence trace on a metal object.
Background
In criminal material evidence investigation field, need discover and acquire the criminal material evidence that the suspect left over, these material evidences include fingerprint material evidence, palm print material evidence, can reflect the vestige material evidence of DNA speciality, borrow this and establish the crime fact evidence chain, establish the legal evidence basis for punishing the crime.
In the prior art, the inventor has proposed a method for developing fingerprints by using indandione, see chinese patent CN106802292A, in which a bioluminescence developing agent is used to soak or spray a permeable object, then the permeable object is dried at a temperature of 50-120 ℃ and a relative humidity of less than 40%, the dried permeable object is irradiated by a laser with a wavelength of 532 nm and a broad-spectrum half-height width of less than 1 nm, and the surface of the permeable object is controlled to form an illumination exceeding 30 ten thousand lux, and a biological evidence trace on the permeable object can be developed under a light-blocking filter at 540 nm, wherein the bioluminescence developing agent has a raw material formula, in terms of weight percentage, as follows: 0.02-0.5% of indene diketone, 4-10% of ethyl acetate, 0.5-1.5% of glycerol, 5-15.5% of pure alcohol and 73.5-90% of petroleum ether. The patent can be used for showing biological material evidence traces on the surfaces of permeable objects such as napkin paper, toilet paper, thermal sensitive paper, invoice bills, writing paper, cloth, bricks, wood, stones and the like, but the biological fluorescence showing agent of the patent cannot show the biological material evidence traces on metal objects.
Disclosure of Invention
The invention aims to provide a bioluminescence developing agent capable of developing biological evidence traces on a metal object.
Another object of the present invention is to provide a method for visualizing traces of biological evidence on a metal object.
The third technical problem to be solved by the invention is to provide an application of the bioluminescence developing agent in developing biological evidence traces on metal objects.
In order to solve the technical problems, the invention adopts the following technical scheme:
the invention aims to provide a bioluminescence developing agent for developing biological evidence traces on a metal object, which comprises the following raw materials in percentage by mass of 100 percent: 1-2% of indene diketone, 15-25% of ethyl acetate, 2-5% of glycerol, 20-25% of pure alcohol and 43-62% of petroleum ether.
Preferably, the formulation of the raw materials of the bioluminescence indicator is as follows according to the mass of the bioluminescence indicator being 100 percent: 1.2-1.8% of indene diketone, 18-22% of ethyl acetate, 3-4% of glycerol, 22-24% of pure alcohol and 48.2-55.8% of petroleum ether.
Further preferably, the formulation of the raw materials of the bioluminescence indicator is as follows, wherein the mass of the bioluminescence indicator is 100 percent: 1.4-1.6% of indene diketone, 19-21% of ethyl acetate, 3.4-3.7% of glycerol, 22.5-23.5% of pure alcohol and 50.2-53.7% of petroleum ether.
The invention also aims to provide a method for showing biological evidence traces on a metal object, which comprises the steps of soaking or spraying the metal object by using the biological fluorescence showing agent, irradiating the metal object by using laser with the wavelength of 532 nm and the spectrum full width at half maximum of less than 1 nm, controlling the surface of the metal object to form illumination of over 30 kilo-lux, and showing the biological evidence traces on the metal object under a cut-off filter lens at 540 nm.
Preferably, after the metal object is soaked or sprayed by the bioluminescence developing agent, the laser is used for irradiating after the surface of the metal object is dried.
Wherein, the drying of the surface of the metal object refers to the volatilization of a reagent on the surface of the metal object, and the heating volatilization or natural volatilization can be specifically adopted.
Further preferably, the metal guest surface is dried in an environment of not more than 50 ℃.
Still preferably, the metal guest surface is dried in an environment not exceeding 45 ℃.
More preferably, the metal guest surface is dried in an environment not exceeding 40 ℃.
Most preferably, the surface of the metal object is dried in an environment of 20-37 ℃.
The third purpose of the invention is to provide the application of the bioluminescence developing agent in the development of biological evidence traces on metal objects.
In the invention, the biological material evidence trace comprises a fingerprint, and the fingerprint comprises a fingerprint and a palm print.
In the present invention, pure alcohol refers to absolute ethanol with a mass concentration of more than 99.7%.
In the invention, the metal objects comprise a steel wire mesh, an iron wire mesh, a hammer, a steel bar, a saw, a steel pipe and the like.
Due to the implementation of the technical scheme, compared with the prior art, the invention has the following advantages:
according to the invention, the biological material evidence trace on the metal object can be shown by adjusting the formula proportion of the biological fluorescence developing agent, so that the extraction of genetic materials is facilitated; in addition, the invention does not need to be dried at high temperature and control the humidity of the object and the environment, thereby simplifying the display aspect and avoiding the damage to the biological material evidence on the object.
Drawings
FIG. 1 is a photograph of a fingerprint of example 1;
FIG. 2 is a photograph of a fingerprint of example 2;
FIG. 3 is a photograph of a fingerprint of example 3;
FIG. 4 is a photograph of a fingerprint of comparative example 1;
fig. 5 is a fingerprint photograph of comparative example 2.
Detailed Description
The present invention will be described in further detail with reference to specific examples, but the present invention is not limited to these examples.
Example 1
(1) And preparation of the biological fluorescence developing agent: adding 3.5g of glycerol into 23g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; sufficiently dissolving 1.5g of indandione in 20g of ethyl acetate to obtain a solution 2; and mixing the solution 1 and the solution 2, adding 52g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) Spraying the steel bar by using the biological fluorescence developing agent prepared in the step (1), irradiating the steel bar by using laser with the wavelength of 532 nm and the spectrum full width at half maximum of less than 1 nm after the adhering agent is volatilized at room temperature (25 ℃), controlling the illumination of 30 kilogrammes formed on the surface of the steel bar, and obtaining a fingerprint shown in figure 1 by a photographic mode under a cut-off filter at 540 nm.
Example 2
(1) And preparation of the biological fluorescence developing agent: adding 4g of glycerol into 24g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; sufficiently dissolving 1.2g of indandione in 18g of ethyl acetate to obtain a solution 2; and mixing the solution 1 and the solution 2, adding 52.8g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) Spraying the steel pipe with the biological fluorescence developing agent prepared in the step (1), irradiating the steel pipe with laser with the wavelength of 532 nm and the spectrum full width at half maximum of less than 1 nm after the adhering agent volatilizes at room temperature (30 ℃), controlling the surface of the steel pipe to form the illumination of 30 kilograms, and obtaining the fingerprint shown in figure 2 by a photographic mode under a cut-off filter at 540 nm.
Example 3
(1) And preparation of the biological fluorescence developing agent: adding 3g of glycerol into 22g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; sufficiently dissolving 1.8g of indandione in 22g of ethyl acetate to obtain a solution 2; and mixing the solution 1 and the solution 2, adding 51.2g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) Spraying the hammer with the bioluminescence developing agent prepared in the step (1), irradiating the hammer with laser with the wavelength of 532 nm and the spectrum full width at half maximum of less than 1 nm at room temperature (40 ℃) after the adhering agent volatilizes, controlling the surface of the hammer to form the illumination of 30 kilo kilograms, and obtaining a fingerprint shown in figure 3 by a photographic mode under a cut filter lens at 540 nm.
Comparative example 1
(1) And preparation of the biological fluorescence developing agent: adding 6g of glycerol into 26g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; 0.8g of indandione is fully dissolved in 10g of ethyl acetate to prepare a solution 2; and mixing the solution 1 and the solution 2, adding 57.2g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) Spraying the steel pipe with the bioluminescence developing agent prepared in the step (1), irradiating the steel pipe with laser with the wavelength of 532 nm and the spectrum full width at half maximum of less than 1 nm at room temperature (25 ℃) after the adhering agent volatilizes, controlling the illumination of 30 kilogrammes formed on the surface of the steel pipe, and obtaining a fingerprint shown in figure 4 by a photographic mode under a cut-off filter at 540 nm.
Comparative example 2
(1) And preparation of the biological fluorescence developing agent: adding 1g of glycerol into 15g of pure alcohol, and fully stirring and dissolving to obtain a solution 1; sufficiently dissolving 2.5g of indandione in 30g of ethyl acetate to obtain a solution 2; and mixing the solution 1 and the solution 2, adding 51.5g of petroleum ether, stirring and dissolving to prepare the bioluminescence developing agent.
(2) Spraying the wire netting with the biological fluorescence developing agent prepared in the step (1), irradiating the wire netting with laser with the wavelength of 532 nanometers and the spectrum full width at half maximum of less than 1 nanometer after the adhering agent volatilizes at room temperature (25 ℃), controlling the surface of the wire netting to form the illumination of 30 kilogrammes, and obtaining the fingerprint shown in figure 5 by a photographic mode under a cut-off filter at 540 nanometers.
The present invention has been described in detail in order to enable those skilled in the art to understand the invention and to practice it, and it is not intended to limit the scope of the invention, and all equivalent changes and modifications made according to the spirit of the present invention should be covered by the present invention.
Claims (8)
1. A bioluminescent visualization agent for visualizing traces of biological evidence on a metal object, characterized by: the biological fluorescence developing agent comprises the following raw materials by mass percent based on 100 percent of the biological fluorescence developing agent: 1-2% of indene diketone, 15-25% of ethyl acetate, 2-5% of glycerol, 20-25% of pure alcohol and 43-62% of petroleum ether; the method for showing the biological material evidence traces on the metal object comprises the steps of soaking or spraying the metal object by using the biological fluorescence showing agent, drying the surface of the metal object in an environment of not more than 45 ℃, wherein the drying of the surface of the metal object means that a reagent on the surface of the metal object volatilizes, then irradiating the metal object by using laser with the wavelength of 532 nm and the spectrum half-height width of less than 1 nm, controlling the surface of the metal object to form the illumination of more than 30 ten thousand luxes, and showing the biological material evidence traces on the metal object under a cut-off filter lens at 540 nm.
2. The bioluminescent visualization agent according to claim 1, wherein: the biological fluorescence developing agent comprises the following raw materials by mass percent based on 100 percent of the biological fluorescence developing agent: 1.2-1.8% of indene diketone, 18-22% of ethyl acetate, 3-4% of glycerol, 22-24% of pure alcohol and 48.2-55.8% of petroleum ether.
3. The bioluminescent visualization agent according to claim 2, wherein: the biological fluorescence developing agent comprises the following raw materials by mass percent based on 100 percent of the biological fluorescence developing agent: 1.4-1.6% of indene diketone, 19-21% of ethyl acetate, 3.4-3.7% of glycerol, 22.5-23.5% of pure alcohol and 50.2-53.7% of petroleum ether.
4. The bioluminescent visualization agent according to any one of claims 1 to 3, wherein: the metal object is one of a steel wire mesh, an iron wire mesh, a hammer, a steel bar, a saw and a steel pipe.
5. A method for visualizing a trace of a biological material on a metal object, comprising: soaking or spraying the metal object with the bioluminescence developing agent according to any one of claims 1 to 4, drying the surface of the metal object in an environment of not more than 45 ℃, wherein the drying of the surface of the metal object means that a reagent on the surface of the metal object volatilizes, then irradiating the metal object with laser with the wavelength of 532 nm and the half-height width of the spectrum of less than 1 nm, controlling the surface of the metal object to form the illumination of more than 30 ten thousand luxes, and displaying the biological evidence trace on the metal object under the cut-off filter lens at 540 nm.
6. A visualization method as recited in claim 5, wherein: and drying the surface of the metal object in an environment of not more than 40 ℃.
7. A visualization method as recited in claim 6, wherein: and drying the surface of the metal object in an environment of 20-37 ℃.
8. Use of a bioluminescent visualization agent according to any one of claims 1 to 4 for the visualization of evidence of a biological substance on a metal object.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101556274A (en) * | 2009-04-16 | 2009-10-14 | 吕侠 | Human body vestige visualization reagent, preparation method and application thereof |
| CN104800862A (en) * | 2006-10-05 | 2015-07-29 | 得克萨斯大学体系董事会 | Efficient synthesis of chelators for nuclear imaging and radiotherapy: compositions and applications |
| CN106674028A (en) * | 2016-12-13 | 2017-05-17 | 华南理工大学 | Benzylidene indandione compound and preparation thereof and application in specific imaging of lipid droplet |
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| US6958328B2 (en) * | 2001-04-18 | 2005-10-25 | Ortho-Mcneil Pharmaceutical, Inc | Arylindenopyridines and related therapeutic and prophylactic methods |
| US20040127510A1 (en) * | 2002-04-16 | 2004-07-01 | Heintzelman Geoffrey R. | Arylindenopyridines and arylindenopyrimidines and related therapeutic and prophylactic methods |
| TW201307490A (en) * | 2011-08-05 | 2013-02-16 | shi-yu Wu | Fingerprint-displaying spray and method utilizing spray to display fingerprint |
| CN104605860B (en) * | 2015-03-03 | 2017-03-01 | 苏州晓松科技开发有限公司 | A kind of fingerprint displaying method in matsurface permeability object |
| CN106802292B (en) * | 2017-01-22 | 2019-07-02 | 苏州晓松科技开发有限公司 | A method of showing permeability object biological evidence trace |
| CN106841140B (en) * | 2017-01-22 | 2018-07-24 | 苏州晓松科技开发有限公司 | A kind of biological evidence trace shows extracting method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104800862A (en) * | 2006-10-05 | 2015-07-29 | 得克萨斯大学体系董事会 | Efficient synthesis of chelators for nuclear imaging and radiotherapy: compositions and applications |
| CN101556274A (en) * | 2009-04-16 | 2009-10-14 | 吕侠 | Human body vestige visualization reagent, preparation method and application thereof |
| CN106674028A (en) * | 2016-12-13 | 2017-05-17 | 华南理工大学 | Benzylidene indandione compound and preparation thereof and application in specific imaging of lipid droplet |
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