CN107603909A - Sewage treatment microbial inoculant and its application method - Google Patents
Sewage treatment microbial inoculant and its application method Download PDFInfo
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- CN107603909A CN107603909A CN201710927121.4A CN201710927121A CN107603909A CN 107603909 A CN107603909 A CN 107603909A CN 201710927121 A CN201710927121 A CN 201710927121A CN 107603909 A CN107603909 A CN 107603909A
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Abstract
The present invention provides a kind of sewage treatment microbial inoculant and its application method.The sewage treatment microbial inoculant includes micro- aerobic heterotrophism bacteria preparation and nitrification bacteria preparation, micro- aerobic heterotrophism bacteria preparation includes bacillus subtilis, bacillus licheniformis, whiterot fungi and nitrobacteria, and the nitrification bacteria preparation includes nitrifying bacteria community, side bacillus and denitrification flora.In a kind of sewage treatment microbial inoculant and its application method provided by the invention, it is autotrophic microbe to nitrify mushroom selected in bacteria preparation, micro- aerobic heterotrophism bacteria preparation, carbon source is used as using carbon dioxide, nitrogen source is used as using the inorganic nitride in water, by photosynthesis or change can synthesize acquisition activity when required energy, contain substantial amounts of ammonia nitrogen in trade effluent, microbial inoculum will effectively remove ammonia nitrogen therein.
Description
Technical field
The invention belongs to microorganism sewage water to handle engineering field, more particularly to a kind of sewage treatment microbial inoculant and its user
Method.
Background technology
In micro- aerobic heterotrophism bacteria preparation, nitrification bacteria preparation, high salt tolerant nitrification bacteria preparation, high halophilic fungi preparation, this several class
Between the microbial inoculum that water quality can be lifted, wastewater treatment efficiency differs after a variety of bacteria preparation combinations.Existing bacteria preparation is dirty
Water treatment efficiency is bad.
The content of the invention
To solve the bad technical problem of existing bacteria preparation wastewater treatment efficiency, the present invention provides a kind of sewage disposal effect
Fruit good sewage treatment microbial inoculant and its application method.
Sewage treatment microbial inoculant provided by the invention includes micro- aerobic heterotrophism bacteria preparation and nitrification bacteria preparation, described micro- aerobic different
Bacteria preparation includes bacillus subtilis, bacillus licheniformis, whiterot fungi and nitrobacteria, and the nitrification bacteria preparation includes nitrification
Flora, side bacillus and denitrification flora.
In a kind of preferred embodiment of sewage treatment microbial inoculant provided by the invention, the nitrification bacteria preparation composition is：It is described
Nitrifying bacteria community is hundred million/ml of 40%CFU10, and side bacillus is hundred million/ml of 30%CFU10, and the denitrification flora is 30%CFU10
Hundred million/ml.
In a kind of preferred embodiment of sewage treatment microbial inoculant provided by the invention, micro- aerobic heterotrophism bacteria preparation composition
For：The bacillus subtilis be 30,%CF,U10 hundred million/gram, the bacillus licheniformis be 40,%CF,U10 hundred million/gram, the white rot
Bacterium be 15,%CF,U10 hundred million/gram, the nitrobacteria be 15,%CF,U10 hundred million/gram.
In a kind of preferred embodiment of sewage treatment microbial inoculant provided by the invention, micro- aerobic heterotrophism bacteria preparation with it is described
Nitrify bacteria preparation is using proportioning：Micro- aerobic heterotrophism bacteria preparation：Nitrification bacteria preparation=the 1kg：1L.
In a kind of preferred embodiment of application method of sewage treatment microbial inoculant provided by the invention, comprise the following steps：
Step 1: prepare before microbial inoculum activation：Strain is checked, determines flora growth condition；
Step 2: mix bacterium agent tank activates on a large scale：By micro- aerobic heterotrophism bacteria preparation and nitrification bacteria preparation respectively in microbial inoculum
Cultivated in nutrient solution, and put into nutrient solution and cultivate, then the strain after two kinds of cultures is mixed.
In a kind of preferred embodiment of application method of sewage treatment microbial inoculant provided by the invention, in the step 1, tool
Body comprises the following steps：
Step 1 one, strain idenfication：Check micro- aerobic heterotrophism bacteria preparation and nitrify the production performance of bacteria preparation, without miscellaneous
Bacterium pollutes；
Step 1 two, determine flora growth condition：It is determined by experiment optimum growth temperature, the PH of flora, fermented and cultured
The most suitable composition and ratio of base, the growth curve of flora is drawn, it is determined that most suitable incubation time.
In a kind of preferred embodiment of application method of sewage treatment microbial inoculant provided by the invention, the step 1 also includes
Following steps：
Step 1 one by one, rejuvenation of spawn purifying：Generating function is sexually revised or the strain by living contaminants, using flat board
Method of scoring carries out the purifying or rejuvenation of strain.
In a kind of preferred embodiment of application method of sewage treatment microbial inoculant provided by the invention, in the step 2, tool
Body comprises the following steps：
Step 2 one, bacteria preparation is cultivated respectively：Cultivate micro- aerobic heterotrophism bacteria preparation and described respectively in microbial inoculum tank
Bacteria preparation is nitrified, the nitrification bacteria preparation makes its Acceleration of growth double using N element；
Step 2 two, will nitrification bacteria preparation and micro- aerobic heterotrophic bacteria preparation put into crack aeration in microbial inoculum tank respectively,
Regulation to suitable growth is adjusted；
Step 2 three, cultivated one day in microbial inoculum tank, be sampled to laboratories microscope observation, after reaching expected dynamic,
Both generals 1:1 mixing, you can be transported to progress waste water control at sewage.
In a kind of preferred embodiment of application method of sewage treatment microbial inoculant provided by the invention, the step 2 one is specific
For：
Microbial inoculum nutrient solution is separately added into the microbial inoculum tank, micro- aerobic heterotrophism bacteria preparation nutrient solution is：Water 1t；It is red
Sugared 8kg；Salt 0.5kg；Magnesium sulfate 0.8kg；Potassium dihydrogen phosphate 0.3kg；Albumen powder 2.5kg；Sludge 5L；The nitrification bacteria preparation training
Nutrient solution is：Water 1t；Brown sugar 10kg；Salt 1kg；Urea 1.5kg (makes nitrification bacteria preparation index double)；Magnesium sulfate 0.3kg；Di(2-ethylhexyl)phosphate
Hydrogen potassium 0.3kg；Albumen powder 2.5kg；Sludge 5L.
In a kind of preferred embodiment of application method of sewage treatment microbial inoculant provided by the invention, the step 2 two is specific
For：Its dissolved oxygen is adjusted to 0.3-0.8 or so, temperature is room temperature, and pH value is maintained at 6.5-7.5 or so.
The sewage treatment microbial inoculant and its application method of the present invention has the advantages that：Micro- aerobic heterotrophism bacteria preparation,
Bacteria preparation, high salt tolerant nitrification bacteria preparation, high halophilic fungi preparation are nitrified, between this several class can lift the microbial inoculum of water quality, is carried out
Combination experiment, has finally picked out the optimal microbial inoculum of wastewater treatment efficiency and has been combined as：Micro- aerobic heterotrophism bacteria preparation and nitrifier
Preparation.It is autotrophic microbe to nitrify mushroom selected in bacteria preparation, micro- aerobic heterotrophism bacteria preparation, and carbon is used as using carbon dioxide
Source, using the inorganic nitride in water as nitrogen source, by photosynthesis or change can synthesize acquisition activity when required energy.
Contain substantial amounts of ammonia nitrogen in trade effluent, microbial inoculum will effectively remove ammonia nitrogen therein.
Nitrify the mushroom effect in bacteria preparation：
Bacillus laterosporus：Chitinase is secreted, suppresses growth of pathogenic bacteria, using organic matter as food.Play and suppress pathogen,
The effect decolourized and purified water.
Nitrobacteria：Mainly using nitrogen class material as the main source of energy, can effectively remove ammonia nitrogen in sewage,
Total nitrogen and sulfide.Role is similar to pasture and water.
Denitrifying bacteria：Main consumption alkaline matter, PH, the regulation sewage PH to weak acidic condition of water are reduced, more suitably
The existence of biology and growth and breeding, promote water ecological restoration.
Nitrification bacteria preparation can improve nitrification efficiency, keep nitrification effect steady in a long-term；It can recover rapidly to impact
Denitrification mix caused by load and burst sexual factor；Cushioning effect is played to the ammonia nitrogen of high concentration, assists other micro-
Biology, which plays, preferably adapts to wastewater environment；The system (such as deamination nitrification) of safety coefficient deficiency is minimized.
The mushroom effect of micro- aerobic heterotrophism bacteria preparation
Bacillus subtilis：Amylase and protease, starch-splitting class and protein substance are produced, effectively decomposing has
Machine thing, play a part of decolourizing and purifying water.
Bacillus licheniformis：Anti-microbial type material is produced, extracellular proteinase, amylase, lipase, protein degradation matter class, is formed sediment
Powder enzyme, oil substances.Suppress pathogen, and decolorization can be played, purify water.
Whiterot fungi：Laccase is produced, decomposes lignin and the pigment with aromatic ring structure.Play what is suppressed pathogen and decolourize
Effect.
Nitrobacteria：Mainly using nitrogen class material as the main source of energy, can effectively remove ammonia nitrogen in sewage,
Total nitrogen and sulfide.Role is similar to pasture and water.
Micro- aerobic heterotrophism bacteria preparation can consume superfluous nitrate and phosphate with fast decoupled biomass, mud；It is not direct
The quantity of any aquatile is had an impact, but ingests in water rubbish by microorganism contained by product to create more high-quality water
Body environment, BOD, COD and TSS level are significantly reduced, eliminates stink；Suitable for lake, pond, calacove, canal and water route boat
The water correction in road and ecological balance reparation, mechanical dredging can be greatly reduced and using harmful chemicals thing to avoid secondary pollution.
Brief description of the drawings
Technical scheme in order to illustrate the embodiments of the present invention more clearly, make required in being described below to embodiment
Accompanying drawing is briefly described, it should be apparent that, drawings in the following description are only some embodiments of the present invention, for
For those of ordinary skill in the art, on the premise of not paying creative work, it can also be obtained according to these accompanying drawings other
Accompanying drawing, wherein：
Fig. 1 is the process chart of the application method of sewage treatment microbial inoculant provided by the invention；
The process chart of the step of Fig. 2 is the application method of the sewage treatment microbial inoculant shown in Fig. 1 one；
The process chart of the step of Fig. 3 is the application method of the sewage treatment microbial inoculant shown in Fig. 1 two.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only the part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained all other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
The sewage treatment microbial inoculant includes micro- aerobic heterotrophism bacteria preparation and nitrification bacteria preparation, micro- aerobic heterotrophism bacteria preparation
Including bacillus subtilis, bacillus licheniformis, whiterot fungi and nitrobacteria, the nitrification bacteria preparation includes nitrifying bacteria community, side
Bacillus and denitrification flora.
The nitrification bacteria preparation, which forms, is：The nitrifying bacteria community is hundred million/ml of 40%CFU10, and side bacillus is 30%
Hundred million/ml of CFU10, the denitrification flora are hundred million/ml of 30%CFU10.
Micro- aerobic heterotrophism bacteria preparation, which forms, is：The bacillus subtilis be 30,%CF,U10 hundred million/gram, the lichens
Bacillus be 40,%CF,U10 hundred million/gram, the whiterot fungi be 15,%CF,U10 hundred million/gram, the nitrobacteria is 15%CFU10
Hundred million/gram.
Micro- aerobic heterotrophism bacteria preparation with it is described nitrification bacteria preparation be using proportioning：Micro- aerobic heterotrophism bacteria preparation：
Nitrification bacteria preparation=the 1kg：1L.
Fig. 1 to Fig. 3 in the lump is referred to, wherein Fig. 1 is the technique of the application method of sewage treatment microbial inoculant provided by the invention
Flow chart；The process chart of the step of Fig. 2 is the application method of the sewage treatment microbial inoculant shown in Fig. 1 one；Fig. 3 is shown in Fig. 1
Sewage treatment microbial inoculant application method the step of two process chart.
The application method of described sewage treatment microbial inoculant, comprises the following steps：
Step S1, prepare before microbial inoculum activation：Strain is checked, determines flora growth condition；Specifically comprise the following steps：
Step S11, strain idenfication：Check micro- aerobic heterotrophism bacteria preparation and nitrify the production performance of bacteria preparation, without miscellaneous
Bacterium pollutes；
Step S12, rejuvenation of spawn purifies：Generating function is sexually revised or the strain by living contaminants, drawn using flat board
Collimation method carries out the purifying or rejuvenation of strain；
Step S13, flora growth condition is determined：It is determined by experiment optimum growth temperature, the PH of flora, fermentation medium
Most suitable composition and ratio, the growth curve of flora is drawn, it is determined that most suitable incubation time.
Step S2, mix bacterium agent tank activates on a large scale：By micro- aerobic heterotrophism bacteria preparation and nitrification bacteria preparation respectively in microbial inoculum
Cultivated in nutrient solution, and put into nutrient solution and cultivate, then the strain after two kinds of cultures is mixed；Specifically comprise the following steps：
Step S21, bacteria preparation is cultivated respectively：Cultivate micro- aerobic heterotrophism bacteria preparation and the nitre respectively in microbial inoculum tank
Change bacteria preparation, the nitrification bacteria preparation makes its Acceleration of growth double using N element；
Specifically, in 8m3The microbial inoculum tank in be separately added into microbial inoculum nutrient solution, micro- aerobic heterotrophism bacteria preparation culture
Liquid is：Water 1t；Brown sugar 8kg；Salt 0.5kg；Magnesium sulfate 0.8kg；Potassium dihydrogen phosphate 0.3kg；Albumen powder 2.5kg；Sludge 5L；It is described
Nitrifying bacteria preparation nutrient solution is：Water 1t；Brown sugar 10kg；Salt 1kg；Urea 1.5kg (makes nitrification bacteria preparation index double)；Magnesium sulfate
0.3kg；Potassium dihydrogen phosphate 0.3kg；Albumen powder 2.5kg；Sludge 5L.
Step S22, nitrification bacteria preparation and micro- aerobic heterotrophic bacteria preparation are put into crack aeration in microbial inoculum tank respectively, adjusted
Section to suitable growth is adjusted；
Specifically, its dissolved oxygen is adjusted to 0.3-0.8 or so, and temperature is room temperature, and pH value is maintained at a 6.5-7.5 left sides
It is right.
Step S23, cultivated one day in microbial inoculum tank, be sampled to laboratories microscope observation, will after reaching expected dynamic
Both 1:1 mixing, you can be transported to progress waste water control at sewage.
The sewage treatment microbial inoculant and its application method provided by the invention of the present invention has the advantages that：Micro- aerobic
Heterotrophism bacteria preparation, nitrification bacteria preparation, high salt tolerant nitrification bacteria preparation, high halophilic fungi preparation, this several class can lift the bacterium of water quality
Between agent, combination experiment has been carried out, the optimal microbial inoculum of wastewater treatment efficiency has finally been picked out and has been combined as：Micro- aerobic heterotroph system
Agent and nitrification bacteria preparation.It is autotrophic microbe to nitrify mushroom selected in bacteria preparation, micro- aerobic heterotrophism bacteria preparation, with titanium dioxide
Carbon is as carbon source, using the inorganic nitride in water as nitrogen source, by photosynthesis or change can synthesize acquisition activity when it is required
Energy.Contain substantial amounts of ammonia nitrogen in trade effluent, microbial inoculum will effectively remove ammonia nitrogen therein.
Nitrify the mushroom effect in bacteria preparation：
Bacillus laterosporus：Chitinase is secreted, suppresses growth of pathogenic bacteria, using organic matter as food.Play and suppress pathogen,
The effect decolourized and purified water.
Nitrobacteria：Mainly using nitrogen class material as the main source of energy, can effectively remove ammonia nitrogen in sewage,
Total nitrogen and sulfide.Role is similar to pasture and water.
Denitrifying bacteria：Main consumption alkaline matter, PH, the regulation sewage PH to weak acidic condition of water are reduced, more suitably
The existence of biology and growth and breeding, promote water ecological restoration.
Nitrification bacteria preparation can improve nitrification efficiency, keep nitrification effect steady in a long-term；It can recover rapidly to impact
Denitrification mix caused by load and burst sexual factor；Cushioning effect is played to the ammonia nitrogen of high concentration, assists other micro-
Biology, which plays, preferably adapts to wastewater environment；The system (such as deamination nitrification) of safety coefficient deficiency is minimized.
The mushroom effect of micro- aerobic heterotrophism bacteria preparation
Bacillus subtilis：Amylase and protease, starch-splitting class and protein substance are produced, effectively decomposing has
Machine thing, play a part of decolourizing and purifying water.
Bacillus licheniformis：Anti-microbial type material is produced, extracellular proteinase, amylase, lipase, protein degradation matter class, is formed sediment
Powder enzyme, oil substances.Suppress pathogen, and decolorization can be played, purify water.
Whiterot fungi：Laccase is produced, decomposes lignin and the pigment with aromatic ring structure.Play what is suppressed pathogen and decolourize
Effect.
Nitrobacteria：Mainly using nitrogen class material as the main source of energy, can effectively remove ammonia nitrogen in sewage,
Total nitrogen and sulfide.Role is similar to pasture and water.
Micro- aerobic heterotrophism bacteria preparation can consume superfluous nitrate and phosphate with fast decoupled biomass, mud；It is not direct
The quantity of any aquatile is had an impact, but ingests in water rubbish by microorganism contained by product to create more high-quality water
Body environment, BOD, COD and TSS level are significantly reduced, eliminates stink；Suitable for lake, pond, calacove, canal and water route boat
The water correction in road and ecological balance reparation, mechanical dredging can be greatly reduced and using harmful chemicals thing to avoid secondary pollution.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalent structure or equivalent flow conversion that bright specification and accompanying drawing content are made, or directly or indirectly it is used in other related skills
Art field, is included within the scope of the present invention.
Claims (10)
1. a kind of sewage treatment microbial inoculant, it is characterised in that described micro- aerobic including micro- aerobic heterotrophism bacteria preparation and nitrification bacteria preparation
Heterotrophism bacteria preparation includes bacillus subtilis, bacillus licheniformis, whiterot fungi and nitrobacteria, and the nitrification bacteria preparation includes nitre
Change flora, side bacillus and denitrification flora.
2. according to the sewage treatment microbial inoculant described in claim 1, it is characterised in that the nitrification bacteria preparation, which forms, is：It is described
Nitrifying bacteria community is hundred million/ml of 40%CFU10, and side bacillus is hundred million/ml of 30%CFU10, and the denitrification flora is 30%CFU10
Hundred million/ml.
3. according to the sewage treatment microbial inoculant described in claim 1, it is characterised in that micro- aerobic heterotrophism bacteria preparation composition
For：The bacillus subtilis be 30,%CF,U10 hundred million/gram, the bacillus licheniformis be 40,%CF,U10 hundred million/gram, the white rot
Bacterium be 15,%CF,U10 hundred million/gram, the nitrobacteria be 15,%CF,U10 hundred million/gram.
4. according to the sewage treatment microbial inoculant described in claim 1, it is characterised in that micro- aerobic heterotrophism bacteria preparation with it is described
Nitrify bacteria preparation is using proportioning：Micro- aerobic heterotrophism bacteria preparation：Nitrification bacteria preparation=the 1kg：1L.
5. the application method of any described sewage treatment microbial inoculant in a kind of Claims 1-4, it is characterised in that including as follows
Step：
Step 1: prepare before microbial inoculum activation：Strain is checked, determines flora growth condition；
Step 2: mix bacterium agent tank activates on a large scale：By micro- aerobic heterotrophism bacteria preparation and nitrification bacteria preparation respectively in microbial inoculum culture
Cultivated in liquid, and put into nutrient solution and cultivate, then the strain after two kinds of cultures is mixed.
6. according to the application method of the sewage treatment microbial inoculant described in claim 5, it is characterised in that in the step 1, tool
Body comprises the following steps：
Step 1 one, strain idenfication：Check micro- aerobic heterotrophism bacteria preparation and nitrify the production performance of bacteria preparation, no miscellaneous bacteria is dirty
Dye；
Step 1 two, determine flora growth condition：Optimum growth temperature, the PH of flora are determined by experiment, fermentation medium
Most suitable composition and ratio, the growth curve of flora is drawn, it is determined that most suitable incubation time.
7. according to the application method of the sewage treatment microbial inoculant described in claim 6, it is characterised in that the step 1 also includes
Following steps：
Step 1 one by one, rejuvenation of spawn purifying：Generating function is sexually revised or the strain by living contaminants, using plate streaking
Method carries out the purifying or rejuvenation of strain.
8. according to the application method of the sewage treatment microbial inoculant described in claim 5, it is characterised in that in the step 2, tool
Body comprises the following steps：
Step 2 one, bacteria preparation is cultivated respectively：Cultivate micro- aerobic heterotrophism bacteria preparation and the nitrification respectively in microbial inoculum tank
Bacteria preparation, the nitrification bacteria preparation make its Acceleration of growth double using N element；
Step 2 two, will nitrification bacteria preparation and micro- aerobic heterotrophic bacteria preparation put into crack aeration in microbial inoculum tank respectively, adjust
Adjusted to suitable growth；
Step 2 three, cultivated one day in microbial inoculum tank, laboratories microscope observation is sampled to, after reaching expected dynamic, by two
Person 1:1 mixing, you can be transported to progress waste water control at sewage.
9. according to the application method of the sewage treatment microbial inoculant described in claim 8, it is characterised in that the step 2 one is specific
For：
Microbial inoculum nutrient solution is separately added into the microbial inoculum tank, micro- aerobic heterotrophism bacteria preparation nutrient solution is：Water 1t；Brown sugar
8kg；Salt 0.5kg；Magnesium sulfate 0.8kg；Potassium dihydrogen phosphate 0.3kg；Albumen powder 2.5kg；Sludge 5L；The nitrification bacteria preparation culture
Liquid is：Water 1t；Brown sugar 10kg；Salt 1kg；Urea 1.5kg (makes nitrification bacteria preparation index double)；Magnesium sulfate 0.3kg；Biphosphate
Potassium 0.3kg；Albumen powder 2.5kg；Sludge 5L.
10. according to the application method of the sewage treatment microbial inoculant described in claim 8, it is characterised in that the step 2 two has
Body is：Its dissolved oxygen is adjusted to 0.3-0.8 or so, temperature is room temperature, and pH value is maintained at 6.5-7.5 or so.
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| CN110301338A (en) * | 2019-06-19 | 2019-10-08 | 姚力元 | A kind of biomembrane organic water ploughs implant system and its operating method |
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| CN110627228A (en) * | 2019-11-12 | 2019-12-31 | 天津软银科技有限公司 | Composition for nitrobacteria culture |
| CN113999836A (en) * | 2021-11-03 | 2022-02-01 | 河北天泓环保科技有限公司 | Compound microbial agent for sewage treatment, preparation method and application of compound microbial agent in river treatment and black and odorous water treatment |
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