CN107602221A - A kind of edible fungus culture medium containing lotus leaf and preparation method thereof - Google Patents

A kind of edible fungus culture medium containing lotus leaf and preparation method thereof Download PDF

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Publication number
CN107602221A
CN107602221A CN201710850910.2A CN201710850910A CN107602221A CN 107602221 A CN107602221 A CN 107602221A CN 201710850910 A CN201710850910 A CN 201710850910A CN 107602221 A CN107602221 A CN 107602221A
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parts
lotus leaf
culture medium
edible fungus
fungus culture
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CN201710850910.2A
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Chinese (zh)
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金民
潘昌跃
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Xingyi City Animal Husbandry Development Co Ltd Days Wild Farmers
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Xingyi City Animal Husbandry Development Co Ltd Days Wild Farmers
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Abstract

The invention discloses a kind of edible fungus culture medium containing lotus leaf and preparation method thereof, and it is as follows that it is formulated composition:Cotton stalk:40 50 parts, lotus leaf:20 30 parts, wheat bran:5 10 parts, pulverized limestone:12 parts, land plaster:12 parts, calcium superphosphate:15 parts, urea:15 parts, zymophyte:0.5 1 parts, Liquid Fertilizer:0.5 1 parts, then edible fungus culture medium is made by stock, heap fermentation, sterilizing, lotus leaf is turned waste into wealth, adds edible fungus culture medium made from other auxiliary materials, have good using effect by the present invention.

Description

A kind of edible fungus culture medium containing lotus leaf and preparation method thereof
Technical field
The invention belongs to technical field of edible fungi production, and in particular to a kind of edible fungus culture medium and its system containing lotus leaf Preparation Method.
Background technology
Edible mushroom is one of three big foundation stones that FAO (Food and Agriculture Organization of the United Nation) advocates " meat or fish, an element, a mushroom " health diet, It is the traditional exported product in China.China's Edible Fungi more using the organic waste of agriculture and forestry product as culturing raw material, its Middle cotton seed hulls, wood chip are main materials.With the fast development of mushroom industry, including edible mushroom in itself, edible fungus health-care product, The marketization of the products such as edible mushroom non-staple food, edible fungi beverage, Edible Fungi enterprise encounter that there is lack of raw materials, spurt in prices etc. Restraining factors.Therefore, how to seek more cheap and suitable cultivation edible mushroom resources, put in face of every planting household A difficult problem.
Lotus leaf is distributed in China mostly as the blade that lotus leaf is Nymphaeceae (Nymphaeaceae) water plant lotus Side, main product are cheap in Hunan, Hubei, Zhejiang, Jiangsu and Fujian and other places.Lotus leaf cultivation or wild in pond, paddy field, Resourceful lotus leaf rots naturally mostly, and only fraction lotus leaf is used as food or medical treatment and its healthcare industry, absolutely mostly Number is not efficiently utilized.
The content of the invention
For edible fungi substrate lack of material in the prior art be present, lotus leaf resource largely wastes, it is impossible to is used as eating The problems such as bacterium matrix, the present invention provide a kind of edible fungus culture medium containing lotus leaf and preparation method thereof, have good use Effect and economic value.
The technical solution adopted by the present invention:Edible fungus culture medium containing lotus leaf, it is as follows that it is formulated composition:Cotton stalk: 40-50 parts, lotus leaf:20-30 parts, wheat bran:5-10 parts, pulverized limestone:1-2 parts, land plaster:1-2 parts, calcium superphosphate:1-5 parts, urine Element:1-5 parts, zymophyte:0.5-1 parts, Liquid Fertilizer:0.5-1 parts.
Edible fungus culture medium containing lotus leaf, it is preferable that its composition is as follows:Cotton stalk:45 parts, lotus leaf:25 parts, wheat bran: 10 parts, pulverized limestone:1 part, land plaster:1 part, calcium superphosphate:2 parts, urea:2 parts, zymophyte:0.5 part, Liquid Fertilizer:1 part.
Lotus leaf is obtained to dry after being extracted with hot water, specially first with after the extraction of meta-alkalescence hot water, then with slant acidity hot water Extract, dried after the material filtering after extraction.
Its formula also includes seawood meal 1-2 parts.
Zymophyte is EM zymophytes, CM zymophytes and ferment bacterium one kind therein.
It is preferred that zymophyte is EM zymophytes.
It is preferred that Liquid Fertilizer is amino acid Water soluble fertilizer.
The preparation method of edible fungus culture medium of the invention containing lotus leaf, comprises the following steps:
(1) stock up:Cotton stalk and lotus leaf are dried, are crushed to 0.2-5mm particle;
(2) heap fermentation:The hybrid particles of above-mentioned cotton stalk and lotus leaf are soaked in water 12-24 hours, then by its Its raw material mixes, and moisture content ensures in 65-70% after mixing, then is fermented;
(3) sterilize:By the steam normal-pressure sterilization of the material after fermentation, 100 DEG C of temperature sterilizes 24 hours.
It is preferred that the detailed process of heap fermentation is in step (2):Mixed material is piled into bottom width 1.0-1.5m, high 0.8- 1.2m, long unlimited bar material heap, are fermented with rice straw mulching, when temperature reaches more than 60 DEG C in material, maintain fermentation 36-48 First time turning is carried out after hour, during turning in towards turn up, on turn over down, fermentation, turning repeat 2-3 times, obtain culture matrix.
The beneficial effect that the present invention is reached compared with prior art:
(1) China's lotus leaf aboundresources, widely distributed, cheap, only very small part is used for medical treatment, food And health products trade, the overwhelming majority rot in lotus pool naturally, the not only wasting of resources, also in the presence of certain environmental pollution;Cause Flavone compound and alkaloid in lotus leaf to be present, and this two classes material has bacteriostasis, so lotus leaf can not be used directly Make edible fungus culture medium, the present invention dried after lotus leaf is extracted with hot water it is obtained, specially first with after the extraction of meta-alkalescence hot water, Flavone compound is removed, then is extracted with slant acidity hot water, alkaloid is removed, is used after being dried after the material filtering after extraction, Extracting obtained flavone compound and alkaloids substance has good medical value.
(2) present invention is additionally added a certain amount of Liquid Fertilizer, particularly Water soluble fertilizer containing amino acid, can be provided for hypha of edible fungus Quick-acting, comprehensive, balanced nutritional ingredient, promote strain to sprout and accelerate to grow, and in the collective effect of pulverized limestone and land plaster Under, living contaminants is effectively prevented and kill off, improves bacterium germination success rate.
(3) present invention can also add a small amount of seawood meal:Seawood meal is using marine natural marine alga as major ingredient, is aided with a small amount of Marine microalgae retrofit forms.Natural seaweed powder rich in algal polysaccharides, mannitol, amino acid, protein, vitamin and potassium, The trace elements such as iron, calcium, phosphorus, iodine, selenium, cobalt, further meet the nutritional requirement of edible fungi substrate.
(4) shown by test data, edible fungi substrate of the invention is cultivated to pleurotus eryngii, has good reality Border using effect, biological conversion rate is up to 91-94%.
Embodiment
Embodiment 1
Edible fungus culture medium of the invention containing lotus leaf, its composition are as follows:Cotton stalk:45kg, the lotus that hot water extracted Leaf:25kg, wheat bran:10kg, pulverized limestone:1kg, land plaster:1kg, calcium superphosphate:2kg, urea:2kg, EM zymophyte:0.5kg, Amino acid Water soluble fertilizer:1kg, seawood meal 1kg.
The preparation method of edible fungus culture medium of the invention containing lotus leaf, comprises the following steps:
(1) stock up:Cotton stalk and lotus leaf are dried, are crushed to 0.2-0.5mm particle;
(2) heap fermentation:The hybrid particles of above-mentioned cotton stalk and lotus leaf are soaked in water 24 hours, then by other originals Material mixing, moisture content ensures in 65-70% after mixing, and it is unlimited that mixed material is piled into bottom width 1.0-1.5m, high 0.8-1.2m, length Bar material heap, is fermented with rice straw mulching, and when temperature reaches more than 60 DEG C in material, maintenance fermentation is carried out for the first time after 48 hours Turning, during turning in towards turn up, on turn over down, fermentation and turning are repeated 3 times, and obtain culture matrix;
(3) sterilize:By the steam normal-pressure sterilization of the material after fermentation, 100 DEG C of temperature sterilizes 24 hours.
Embodiment 2
Edible fungus culture medium of the invention containing lotus leaf, its composition are as follows:Cotton stalk:40kg, the lotus that hot water extracted Leaf:30kg, wheat bran:5kg, pulverized limestone:1kg, land plaster:2kg, calcium superphosphate:1kg, urea:1kg, ferment bacterium:0.5kg, ammonia Base acid Water soluble fertilizer:0.5kg.
The preparation method of edible fungus culture medium of the invention containing lotus leaf, comprises the following steps:
(1) stock up:Cotton stalk and lotus leaf are dried, are crushed to 1-5mm particle;
(2) heap fermentation:The hybrid particles of above-mentioned cotton stalk and lotus leaf are soaked in water 12 hours, then by other originals Material mixing, moisture content ensures in 65-70% after mixing, and it is unlimited that mixed material is piled into bottom width 1.0-1.5m, high 0.8-1.2m, length Bar material heap, is fermented with rice straw mulching, and when temperature reaches more than 60 DEG C in material, maintenance fermentation is carried out for the first time after 36 hours Turning, during turning in towards turn up, on turn over down, fermentation and turning are repeated 2 times, and obtain culture matrix;
(3) sterilize:By the steam normal-pressure sterilization of the material after fermentation, 100 DEG C of temperature sterilizes 24 hours.
Embodiment 3
Edible fungus culture medium of the invention containing lotus leaf, its composition are as follows:Cotton stalk:50kg, the lotus that hot water extracted Leaf:20kg, wheat bran:10kg, pulverized limestone:2kg, land plaster:2kg, calcium superphosphate:5kg, urea:5kg, CM zymophyte:1kg, it is rotten Grow sour Water soluble fertilizer:1kg.
The preparation method of edible fungus culture medium of the invention containing lotus leaf, comprises the following steps:
(1) stock up:Cotton stalk and lotus leaf are dried, are crushed to 0.2-2mm particle;
(2) heap fermentation:The hybrid particles of above-mentioned cotton stalk and lotus leaf are soaked in water 18 hours, then by other originals Material mixing, moisture content ensures in 65-70% after mixing, and it is unlimited that mixed material is piled into bottom width 1.0-1.5m, high 0.8-1.2m, length Bar material heap, is fermented with rice straw mulching, and when temperature reaches more than 60 DEG C in material, maintenance fermentation is carried out for the first time after 48 hours Turning, during turning in towards turn up, on turn over down, fermentation and turning are repeated 2 times, and obtain culture matrix;
(3) sterilize:By the steam normal-pressure sterilization of the material after fermentation, 100 DEG C of temperature sterilizes 24 hours.
Embodiment 4
Planting almond abalone mushroom is tested:
(1) it is inoculated with, the edible fungi substrate bag to have sterilized is moved on in transfer room, after carrying out sterilizing to transfer room, food 30 DEG C etc. are down to bacterium matrix bag temperature to be inoculated with, and cultivate mycelia, observation, record at room temperature at 23-25 DEG C.Often handle 50 Bag, 3 repetitions.
(2) after bacterium germination and the long purseful of management of producing mushroom mycelia, move into mushroom room and carry out management of producing mushroom, and observe, record.
(3) the different strain of Pleurotus eryngii fruiting yield of output statistics record statistics, calculating average product, biological efficiency (with Machine extracts 30 bags of bacterium bag siccative quality, moushroom yields, calculation biology efficiency, takes its average value), test data is shown in Table 1.Wherein, The edible fungi substrate of CK controls is using common corncob 100kg, beancake powder 5kg, pulverized limestone 5kg, land plaster 2kg, peroxophosphoric acid Calcium 4kg, urea 0.5kg, it is made using being sterilized after heap fermentation.Pleurotus eryngii quel strains are provided by variety source research institute of Guizhou Province.
The culture medium of edible fungus Xinbao mushroom culturing result of the present invention of table 1

Claims (9)

  1. A kind of 1. edible fungus culture medium containing lotus leaf, it is characterised in that:It is as follows that it is formulated composition:Cotton stalk:40-50 parts, Lotus leaf:20-30 parts, wheat bran:5-10 parts, pulverized limestone:1-2 parts, land plaster:1-2 parts, calcium superphosphate:1-5 parts, urea:1-5 parts, Zymophyte:0.5-1 parts, Liquid Fertilizer:0.5-1 parts.
  2. A kind of 2. edible fungus culture medium containing lotus leaf as claimed in claim 1, it is characterised in that:It is as follows that it is formulated composition: Cotton stalk:45 parts, lotus leaf:25 parts, wheat bran:10 parts, pulverized limestone:1 part, land plaster:1 part, calcium superphosphate:2 parts, urea:2 Part, zymophyte:0.5 part, Liquid Fertilizer:1 part.
  3. A kind of 3. edible fungus culture medium containing lotus leaf as claimed in claim 1, it is characterised in that:Lotus leaf is to be extracted with hot water After dry it is obtained.
  4. A kind of 4. edible fungus culture medium containing lotus leaf as claimed in claim 1, it is characterised in that:Its formula also includes marine alga Powder 1-2 parts.
  5. A kind of 5. edible fungus culture medium containing lotus leaf as claimed in claim 1, it is characterised in that:Zymophyte is fermented for EM One kind in bacterium, CM zymophytes and ferment bacterium.
  6. A kind of 6. edible fungus culture medium containing lotus leaf as claimed in claim 5, it is characterised in that:Zymophyte is fermented for EM Bacterium.
  7. A kind of 7. edible fungus culture medium containing lotus leaf as claimed in claim 1, it is characterised in that:Liquid Fertilizer is amino sour water Molten fertilizer.
  8. A kind of 8. preparation method of the edible fungus culture medium containing lotus leaf as claimed in claim 1, it is characterised in that:Including with Lower step:
    (1) stock up:Cotton stalk and lotus leaf are dried, are crushed to 0.2-5mm particle;
    (2) heap fermentation:The hybrid particles of above-mentioned cotton stalk and lotus leaf are soaked in water 12-24 hours, then by other originals Material mixing, moisture content ensures in 65-70% after mixing, then is fermented;
    (3) sterilize:By the steam normal-pressure sterilization of the material after fermentation, 100 DEG C of temperature sterilizes 24 hours.
  9. A kind of 9. preparation method of the edible fungus culture medium containing lotus leaf as claimed in claim 8, it is characterised in that:Accumulation hair The detailed process of ferment is:Mixed material is piled into bottom width 1.0-1.5m, high 0.8-1.2m, long unlimited bar material heap, uses straw Covering is fermented, and when temperature reaches more than 60 DEG C in material, maintenance carries out turning after fermentation 36-48 hours, fermentation, turning repeat 2-3 times, obtain culture matrix.
CN201710850910.2A 2017-05-31 2017-09-20 A kind of edible fungus culture medium containing lotus leaf and preparation method thereof Pending CN107602221A (en)

Applications Claiming Priority (2)

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CN2017104058927 2017-05-31
CN201710405892 2017-05-31

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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101870611A (en) * 2010-06-25 2010-10-27 汉枫缓释肥料(江苏)有限公司 Amino acid-containing water-soluble fertilizer with novel biological agent and preparation method thereof
CN102875239A (en) * 2012-10-22 2013-01-16 杭州市农业科学研究院 Culture medium for edible fungi and production method of culture medium
CN102964175A (en) * 2012-11-12 2013-03-13 北京依科曼生物技术有限公司 Composite amino-acid-containing water-soluble fertilizer and preparation method thereof
CN104151066A (en) * 2014-07-31 2014-11-19 陆良爨乡绿园菇业有限公司 Pleurotus eryngii culture medium and preparation method thereof
CN104163706A (en) * 2014-07-28 2014-11-26 安庆市绿茵农业发展有限公司 Pleurotus eryngii culture medium containing vermiculite powder and preparation method thereof
CN104291968A (en) * 2014-09-25 2015-01-21 安徽天都灵芝制品公司 Fermented straw pleurotus eryngii culture medium and preparation method thereof
CN106187380A (en) * 2016-07-12 2016-12-07 东至县杨套食用菌产销专业合作社 A kind of bagasse is Pleurotus eryngii bacterium rod of raw material and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101870611A (en) * 2010-06-25 2010-10-27 汉枫缓释肥料(江苏)有限公司 Amino acid-containing water-soluble fertilizer with novel biological agent and preparation method thereof
CN102875239A (en) * 2012-10-22 2013-01-16 杭州市农业科学研究院 Culture medium for edible fungi and production method of culture medium
CN102964175A (en) * 2012-11-12 2013-03-13 北京依科曼生物技术有限公司 Composite amino-acid-containing water-soluble fertilizer and preparation method thereof
CN104163706A (en) * 2014-07-28 2014-11-26 安庆市绿茵农业发展有限公司 Pleurotus eryngii culture medium containing vermiculite powder and preparation method thereof
CN104151066A (en) * 2014-07-31 2014-11-19 陆良爨乡绿园菇业有限公司 Pleurotus eryngii culture medium and preparation method thereof
CN104291968A (en) * 2014-09-25 2015-01-21 安徽天都灵芝制品公司 Fermented straw pleurotus eryngii culture medium and preparation method thereof
CN106187380A (en) * 2016-07-12 2016-12-07 东至县杨套食用菌产销专业合作社 A kind of bagasse is Pleurotus eryngii bacterium rod of raw material and preparation method thereof

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Application publication date: 20180119