CN107595909A - A kind of method that flavone compound is extracted from Folium Panacis Quinquefolii - Google Patents
A kind of method that flavone compound is extracted from Folium Panacis Quinquefolii Download PDFInfo
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- CN107595909A CN107595909A CN201711101290.9A CN201711101290A CN107595909A CN 107595909 A CN107595909 A CN 107595909A CN 201711101290 A CN201711101290 A CN 201711101290A CN 107595909 A CN107595909 A CN 107595909A
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Abstract
The present invention relates to a kind of method that flavone compound is extracted from Folium Panacis Quinquefolii, comprise the following steps：1) Feedstock treating：Folium Panacis Quinquefolii after collection is washed, the Folium Panacis Quinquefolii after cleaning is placed in ventilation and dried；Dried Folium Panacis Quinquefolii is ground into powder；2) extract：Food is dissolved in the water the Extraction solvent that obtained concentration is below 15mmol/L with Tween 80, Folium Panacis Quinquefolii powder is added in Extraction solvent, the liquid-solid ratio for controlling Extraction solvent and Folium Panacis Quinquefolii powder is (15~25)：1ml/g, after be placed under water-bath or Ultrasonic Conditions and extracted, the temperature for controlling extraction is 50~80 DEG C, and gained extract solution is filtered after cooling, and gained filtrate is merged, and flavone compound is in gained filtrate.The present invention can be reduced extraction cost, improve recovery rate, be a kind of new, efficient and easy extracting method using the flavone compound in surfactant solution extraction Folium Panacis Quinquefolii.
Description
Technical field
The present invention relates to a kind of extracting method of flavone compound, more particularly to one kind to extract flavones from Folium Panacis Quinquefolii
The method of class compound, belong to the extractive technique field of natural products.
Background technology
American Ginseng (Panax quinquefolium L.) is Araliaceae ginseng, belongs to herbaceos perennial, originates in and adds
Put on airs and the U.S., planted in China's large area, its medicinal history is long, is traditional rare help medicinal material, its study and
Using by most attention both domestic and external.The product of association, its production cost ratio during Folium Panacis Quinquefolii is planted for American ginseng medicine
American ginseng root is low, and with utilization of the people to Folium Panacis Quinquefolii resource, Folium Panacis Quinquefolii turns into a kind of independent Chinese medicine.When
The present, the cultivated area of American Ginseng also expanded year by year, but has a large amount of American Ginsengs every year because domestic and international American Ginseng demand increases severely
Leaf is discarded in field as discarded object and is not efficiently used, therefore, utilization Folium Panacis Quinquefolii not only have aboundresources,
The advantages that safe ready, noresidue, it can also bring huge economic value and social value.
General flavone is a kind of native compound, is the active ingredient in many Chinese herbal medicines.Flavone compound is with flavones
(2- phenyl chromone) is parent nucleus and derivative a kind of xanthein, isomer and its hydrogenation including flavones and also
Thing is originated in, namely with C6-C3-C6For a series of compounds of basic carbon skeleton.Flavone compound is very wide in plant kingdom's distribution,
Most of form for being combined into glycoside or carbon glycosyl with sugar is present in plant, and also some exists in a free form.With freedom
The development of base life science, the strong elimination free radical and antioxidation of flavone compound receive high attention.Flavones
The phosphorylation of class compound participation phosphoric acid and protein, calcium ion transfer, arachidonic acid metabolic, radicals scavenging, chelating are made
With, the enhancing of redox, anti-oxidant vigor and the expression of gene, and can with antiallergy, anti-inflammatory, suppress bacterium,
Suppress parasite, suppress virus etc..Natural biological flavone molecular weight is small, can be absorbed into adipose tissue rapidly by human body, side
Help others body dispelling fatigue, protection blood vessel, defence radiation.
Our isolated 2 flavones ingredients from Folium Panacis Quinquefolii, through physics and chemistry and Spectrum Analysis confirmation for Kaempferol and
Panasenoside.The mass fraction of flavones in Folium Panacis Quinquefolii is determined using HPLC technologies, measurement result shows the west of different sources
The mass fraction of ginseng flavones is different but more than 0.2% in American ginseng leaf, illustrates in Folium Panacis Quinquefolii except containing ginsenoside
Outside composition, the mass fraction of flavone compound is also higher.
After above-mentioned result is obtained, how the flavone compound in Folium Panacis Quinquefolii to be extracted be used be pendulum
New problem in face of us, traditional extracting method be using organic solvent extract, such as alcohol extracting, but this method exist effectively into
Point and organic solvent effect after, the structure of active ingredient may be destroyed, so as to lose drug effect, and consumption of organic solvent it is big,
The problems such as having loss in recovery difficulty and removal process, while cost increase and organic solvent residual can be caused.It is although new
Extractive technique such as supercritical extract, high pressure assisted extraction method, microwave―assisted extraction etc. have " green " it is environmentally friendly the advantages of,
But use the shortcomings of requirement of technology is high, cost of equipment is expensive.
Surfactant refers to the material that can be remarkably decreased target solution surface tension, and its molecular structure has amphiphilic
Property.Surfactant can extract the active ingredient of plant with solubilization, wetting action and peptizaiton.Thus utilize surface
Activator solution extracts Folium Panacis Quinquefolii chromocor compound, can reduce extraction cost, improve recovery rate, be it is a kind of it is new, efficiently and
Easy extracting method.
The content of the invention
The present invention for existing extracting method be applied to from Folium Panacis Quinquefolii extract flavone compound when it is existing not
A kind of foot, there is provided method that flavone compound is extracted from Folium Panacis Quinquefolii.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
A kind of method that flavone compound is extracted from Folium Panacis Quinquefolii, comprises the following steps：
1) Feedstock treating：Folium Panacis Quinquefolii after collection is washed, to remove the agricultural chemicals and soil of residual；By the west after cleaning
American ginseng leaf is placed in ventilation and dried, untill weighing data is constant twice before and after；Dried Folium Panacis Quinquefolii is ground into powder
End, and be fitted into stand-by in closed container；
2) extract：Food is dissolved in the water the Extraction solvent that obtained concentration is below 15mmol/L with Tween-80, will be walked
It is rapid 1) obtained by Folium Panacis Quinquefolii powder be added in Extraction solvent, the liquid-solid ratio for controlling Extraction solvent and Folium Panacis Quinquefolii powder is
(15~25)：1ml/g, after be placed under water-bath or Ultrasonic Conditions and extracted, the temperature for controlling extraction is 50~80 DEG C,
Gained extract solution is filtered after cooling, and gained filtrate is merged, and flavone compound is in gained filtrate.
Further, ultrasonic power is 100~450W.
Further, the time of extraction is 30~90min.
Further, extraction time is 1~3 time.
The assay method of general flavone content is as follows in above-mentioned gained filtrate：
1) filtrate is placed in 100mL volumetric flasks, constant volume obtains ginseng leaf extract；
2) measure of general flavone content：Absorption distilled water 3.1ml, 95% ethanol 1.5ml are added in each test tube, then are added
Enter ginseng leaf extract 0.2ml, 10%AlCl3Solution 0.1ml and 1mol/L potassium acetate solution 0.1ml, be well mixed after
40min is reacted under dark surrounds, its absorbance is measured under 415nm wavelength.The regression equation C=334.31A- of standard curve
2.6869 (in formula, C is mass concentration, mg/L；A is absorbance), coefficient R2=1.
The beneficial effects of the invention are as follows：
1) in the product that method of the invention extracts the amount of flavone compound with use pure water or ethanol through identical
The product of method processing gained is compared, and improves more than 27%, extraction efficiency is higher；
2) present invention can be reduced and be extracted into using the flavone compound in surfactant solution extraction Folium Panacis Quinquefolii
Originally recovery rate, is improved, is a kind of new, efficient and easy extracting method.
Embodiment
The principle and feature of the present invention are described below in conjunction with example, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.
Embodiment 1：
1) Folium Panacis Quinquefolii after collection is washed twice, to remove the agricultural chemicals and soil of residual；By the American Ginseng after cleaning
Leaf is placed in ventilation and dried, untill weighing data is constant twice before and after；Dried Folium Panacis Quinquefolii is ground into powder, and
It is fitted into stand-by in closed container；
2) extract：Food is dissolved in the water the Extraction solvent that obtained concentration is 5mmol/L with Tween-80, by step 1)
The Folium Panacis Quinquefolii powder of gained is added in Extraction solvent, and the liquid-solid ratio for controlling Extraction solvent and Folium Panacis Quinquefolii powder is 15ml/
G, after be respectively placed in water-bath or supersonic frequency is 40kHz, power is 300w Ultrasonic Conditions under extracted, control carries
The temperature taken is 50 DEG C, and the time of extraction is 30min, and gained extract solution filters after cooling under water-bath and Ultrasonic Conditions, will
Gained filtrate merges, and flavone compound is in gained filtrate.
Embodiment 2：
1) Folium Panacis Quinquefolii after collection is washed twice, to remove the agricultural chemicals and soil of residual；By the American Ginseng after cleaning
Leaf is placed in ventilation and dried, untill weighing data is constant twice before and after；Dried Folium Panacis Quinquefolii is ground into powder, and
It is fitted into stand-by in closed container；
2) extract：Food is dissolved in the water the Extraction solvent that obtained concentration is 10mmol/L with Tween-80, by step 1)
The Folium Panacis Quinquefolii powder of gained is added in Extraction solvent, and the liquid-solid ratio for controlling Extraction solvent and Folium Panacis Quinquefolii powder is 20ml/
G, after be respectively placed in water-bath or supersonic frequency is 40kHz, power is 100w Ultrasonic Conditions under extracted, control carries
The temperature taken is 65 DEG C, and the time of extraction is 90min, is extracted respectively twice, gained extract solution warp under water-bath and Ultrasonic Conditions
Filtered after cooling, gained filtrate is merged, flavone compound is in gained filtrate.
Embodiment 3：
1) Folium Panacis Quinquefolii after collection is washed twice, to remove the agricultural chemicals and soil of residual；By the American Ginseng after cleaning
Leaf is placed in ventilation and dried, untill weighing data is constant twice before and after；Dried Folium Panacis Quinquefolii is ground into powder, and
It is fitted into stand-by in closed container；
2) extract：Food is dissolved in the water the Extraction solvent that obtained concentration is 15mmol/L with Tween-80, by step 1)
The Folium Panacis Quinquefolii powder of gained is added in Extraction solvent, and the liquid-solid ratio for controlling Extraction solvent and Folium Panacis Quinquefolii powder is 25ml/
G, after be respectively placed in water-bath or supersonic frequency is 40kHz, power is 450w Ultrasonic Conditions under extracted, control carries
The temperature taken is 80 DEG C, and the time of extraction is 60min, is extracted respectively three times, gained extract solution warp under water-bath and Ultrasonic Conditions
Filtered after cooling, gained filtrate is merged, flavone compound is in gained filtrate.
Comparative example：
1) Folium Panacis Quinquefolii after collection is washed twice, to remove the agricultural chemicals and soil of residual；By the American Ginseng after cleaning
Leaf is placed in ventilation and dried, untill weighing data is constant twice before and after；Dried Folium Panacis Quinquefolii is ground into powder, and
It is fitted into stand-by in closed container；
2) extract：Folium Panacis Quinquefolii powder obtained by step 1) is added to the water, the liquid of control water and Folium Panacis Quinquefolii powder
Gu ratio is 15ml/g, after be respectively placed in water-bath or supersonic frequency is 40kHz, power is 300w Ultrasonic Conditions under carry out
Extraction, the temperature for controlling extraction is 50 DEG C, and the time of extraction is 30min, and gained extract solution is through cold under water-bath and Ultrasonic Conditions
But filter afterwards, gained filtrate is merged, flavone compound is in gained filtrate.
The method of testing of embodiment 1-3 and comparative example gained filtrate is as follows：
Filtrate is placed in 100mL volumetric flasks respectively first, constant volume obtains ginseng leaf extract；Draw afterwards distilled water 3.1ml,
95% ethanol 1.5ml is added in each test tube, adds ginseng leaf extract 0.2ml, 10%AlCl3Solution 0.1ml and
1mol/L potassium acetate solution 0.1ml, 40min is reacted under dark surrounds after well mixed, its suction is measured under 415nm wavelength
Luminosity.(in formula, C is mass concentration to the regression equation C=334.31A-2.6869 of standard curve, mg/L；A is absorbance), phase
Close coefficients R2=1.
The content of embodiment 1-3 and the flavone compound obtained by comparative example is as shown in table 1：
The embodiment 1-3 of table 1 and comparative example gained flavone compound content
By the as shown by data in table 1, Tween 80 concentration is that 5mmol/L, 10mmol/L and 15mmol/L handle water-bath extraction
Folium Panacis Quinquefolii in general flavone content, added respectively compared with undressed Folium Panacis Quinquefolii 1.27 times, 1.56 times and
1.74 again.Meanwhile Tween 80 concentration is in the Folium Panacis Quinquefolii that 5mmol/L, 10mmol/L and 15mmol/L handle ultrasonic wave extraction
The content of general flavone, add 1.57 times, 1.78 times and 1.96 times respectively compared with undressed Folium Panacis Quinquefolii.In a word, it is western
The content of flavone compound is when Tween 80 concentration is between 0~15mmol/L in American ginseng leaf, water bath with thermostatic control extraction and ultrasound
Ripple extraction is increased with the rise of Tween 80 concentration, and the efficiency of especially Tween 80 combination ultrasonic wave extraction is better than water
Bath extraction.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (4)
- A kind of 1. method that flavone compound is extracted from Folium Panacis Quinquefolii, it is characterised in that comprise the following steps：1) Feedstock treating：Folium Panacis Quinquefolii after collection is washed, to remove the agricultural chemicals and soil of residual；By the American Ginseng after cleaning Leaf is placed in ventilation and dried；Dried Folium Panacis Quinquefolii is ground into powder, and is fitted into stand-by in closed container；2) extract：Food is dissolved in the water the Extraction solvent that obtained concentration is below 15mmol/L with Tween-80, by step 1) The Folium Panacis Quinquefolii powder of gained is added in Extraction solvent, control the liquid-solid ratio of Extraction solvent and Folium Panacis Quinquefolii powder for (15~ 25)：1ml/g, after be placed under water-bath or Ultrasonic Conditions and extracted, the temperature for controlling extraction is 50~80 DEG C, gained Extract solution is filtered after cooling, and gained filtrate is merged, and flavone compound is in gained filtrate.
- 2. according to the method for claim 1, it is characterised in that ultrasonic power is 100~450W.
- 3. method according to claim 1 or 2, it is characterised in that the time of extraction is 30~90min.
- 4. according to the method for claim 3, it is characterised in that extraction time is 1~3 time.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114560900A (en) * | 2022-02-17 | 2022-05-31 | 山东省分析测试中心 | Flavanone compound separated from American ginseng stems and leaves and application of flavanone compound in resisting colon cancer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105231249A (en) * | 2015-09-17 | 2016-01-13 | 暨南大学 | Bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent |
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- 2017-11-10 CN CN201711101290.9A patent/CN107595909A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105231249A (en) * | 2015-09-17 | 2016-01-13 | 暨南大学 | Bamboo leaf flavone extracting method by synergy of ultrasonic and surface-active agent |
Non-Patent Citations (2)
| Title |
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| 孟祥颖: "西洋参不同部位中黄酮的含量测定", 《长春中医学院学报》 * |
| 肖道安: "非离子表面活性剂协同超声提取广东紫珠总黄酮的研究", 《日用化学工业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114560900A (en) * | 2022-02-17 | 2022-05-31 | 山东省分析测试中心 | Flavanone compound separated from American ginseng stems and leaves and application of flavanone compound in resisting colon cancer |
| CN114560900B (en) * | 2022-02-17 | 2023-09-12 | 北京东方红航天生物技术股份有限公司 | Dihydroflavonoid compound separated from stems and leaves of American ginseng and application of dihydroflavonoid compound in resisting colon cancer |
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Application publication date: 20180119 |