CN107593773B - Microbial compound bactericide for preventing and treating mulberry white fruit disease and application thereof - Google Patents
Microbial compound bactericide for preventing and treating mulberry white fruit disease and application thereof Download PDFInfo
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Abstract
The invention discloses a microbial compound bactericide for preventing and treating mulberry fruit diseases and application thereof. The bactericide comprises a mixture of trichoderma harzianum and difenoconazole, wherein the mass ratio of the trichoderma harzianum to the difenoconazole is 100: 2 to 15. Further, the bactericide consists of 8-75% of a main agent and 25-92% of an auxiliary agent by mass percent; the main agent is a mixture of trichoderma harzianum and difenoconazole, and the auxiliary agent is a stabilizer, an auxiliary agent and a filler. The compound bactericide has remarkable synergistic effect in preventing and treating the mulberry fruit disease, the morbidity of the mulberry fruit disease after use can be as low as 4.6 percent, the prevention and treatment effect is as high as 91 percent, and the prevention and treatment effect is remarkably superior to that of a single preparation; the dosage of chemical agents is reduced, and the problem of pesticide residue in the mulberry is solved; the compound bactericide has good quick-acting property and persistence, can be absorbed within 2 hours after application, and can obviously improve the yield of the mulberry.
Description
Technical Field
The invention belongs to the technical field of pesticides, and relates to a microbial compound bactericide for preventing and treating mulberry fruit rot and application thereof. More particularly, relates to an environment-friendly, pollution-free and residue-free microorganism compound bactericide of trichoderma harzianum and difenoconazole capable of preventing and treating mulberry fruit diseases and application thereof.
Background
Mulberry fruit blight is commonly known as Sclerotinia sclerotiorum, is a fungus disease on mulberry fruits, and is mainly caused by the fact that pathogenic bacteria such as Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), Morus sabdariffa (Ciboria shiriana), Ciboria carunculoides (Ciboria carunculoides) and Sclerotinula polymorpha (Sclerotinula shiriana) infect flowers of mulberry, and finally mulberry fruits are attacked. The mulberry white fruit disease affects the yield and quality of the mulberry when being mild, and causes the normal maturation of the mulberry when being severe, so that the yield of the mulberry is reduced in a large area, even the mulberry particles are not harvested, the destructive disaster of the mulberry industry is caused, the raw material sources required by the mulberry processing industry and the silkworm industry are directly affected, and the continuous development of the mulberry industry is threatened.
At present, the technical measures of comprehensive agricultural control and chemical control are mainly adopted for controlling the mulberry fruit diseases. The agricultural comprehensive control mainly aims at reducing the base number of pathogenic substances of an initial infection source and cutting off the transmission path of the pathogenic substances, so that the control effect is limited. The frequency of chemical pesticide application for chemical disease control is frequent, and the drug resistance of pathogenic bacteria is easily enhanced by using chemical pesticide in a large area and high dosage for a long time, so that the chemical pesticide residue in mulberry products and the ecological environment seriously exceeds the standard.
The adoption of a pesticide compounding technology or a mixed application technology is one of the important methods for overcoming or delaying the drug resistance of pests. At present, a method for preventing and treating the mulberry fruit diseases by using a plurality of bactericides in turn and mixing is available, but the prevention and treatment method has the problems of high chemical agent consumption, large pesticide residue, easy generation of cross drug resistance of diseases, poor prevention and treatment effect and the like, and the method becomes an important factor for limiting the quality and the yield of the mulberries. The compounding of the microorganism and the chemical pesticide shows better application prospect, but the microorganism and the pesticide in the compound agent have stricter mutual restriction. Therefore, how to achieve non-toxic, efficient and zero-residue prevention and treatment when the mulberry white fruit disease is prevented and treated is the key for solving the practical problems at present and the key for developing the microbial and pesticide compound agent.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects and shortcomings of the existing mulberry fruit disease control technology, and provides a non-toxic, pollution-free, efficient and residue-free microbial compound bactericide for controlling mulberry fruit disease and application thereof. According to the invention, trichoderma harzianum and low-dose difenoconazole are mixed for use, and are absorbed by flowers of mulberries 2 hours after application, so that the flowers of the mulberries can be prevented from being damaged by germs, the quality and the yield of mulberries can be obviously improved, and the prevention and treatment effects of ginkgo diseases are improved.
The invention aims to provide a microbial compound bactericide for preventing and treating mulberry fruit diseases.
The invention also aims to provide application of the microbial compound bactericide in preventing and treating mulberry fruit diseases.
The purpose of the invention is realized by the following technical scheme:
the microbial compound bactericide for preventing and treating mulberry fruit diseases comprises a mixture of trichoderma harzianum and difenoconazole, wherein the mass ratio of the trichoderma harzianum to the difenoconazole is 100: 2 to 15.
Preferably, the Trichoderma harzianum is Trichoderma harzianum strain Th-N5, belongs to Deuteromycotina, Hyphomycetes, Hyphomycetales and Moniliaceae, is separated from naturally infected mulberry ginkgo in Guangzhou region, and is preserved in the China center for type culture Collection (CCTCC No): m2016251.
The bacterial colony of the strain Th-N5 on the PDA culture medium is thick and compact, white floccule at the initial stage and dark green or green at the later stage, and the back of the bacterial colony is yellow or light green; conidiophores grow, grow oppositely or grow mutually from side branches of hyphae, 2-3 secondary branches exist, and the conidiophores are in a bottle shape or a cone shape; the hyphae are fine and colorless, and have separation and multiple branches; conidia are mostly spherical and 2-2.9 multiplied by 1.9-2.6 μm, and single spores are aggregated on peduncles into spheres.
The trichoderma harzianum strain Th-N5 screened by the inventor is a superior strain specially used for preventing and treating mulberry fruit diseases, and has the characteristics of high efficiency, environmental friendliness and the like. Compared with other Trichoderma harzianum, the strain has some unique bacteriostatic activity, has a better prevention and treatment effect on mulberry fruit diseases, and has certain drug resistance on bactericides such as difenoconazole and the like.
The study of the invention finds that the trichoderma harzianum can be used in combination with difenoconazole, and the trichoderma harzianum and the difenoconazole can play a remarkable synergistic effect when used in combination. The systemic and osmotic effects of the difenoconazole are favorable for inhibiting the growth of pathogenic bacteria in plants and improving the prevention and treatment effect of the ginkgo disease; the remarkable synergistic effect can be realized by mixing the trichoderma harzianum and the low-dose difenoconazole, the use amount of the difenoconazole is favorably reduced, the resistance of plant pathogenic bacteria to the difenoconazole is delayed, and meanwhile, the ecological environment is protected. Therefore, screening the mixture of the Trichoderma harzianum and the difenoconazole has important economic significance and ecological benefit.
In order to better implement the invention, the mass ratio of trichoderma harzianum to difenoconazole is preferably trichoderma harzianum: difenoconazole-100: 4 to 10.
Preferably, the trichoderma harzianum is trichoderma harzianum conidium powder.
The microbial compound bactericide has the bactericidal effect mainly by mixing the trichoderma harzianum and the difenoconazole with auxiliary agents according to the conventional technology in the field to obtain the effect of convenient application.
The invention also provides a microbial compound bactericide which is prepared from the mixture of trichoderma harzianum and difenoconazole as a main agent and auxiliary agents. The microbial compound bactericide consists of 8-75% of a main agent and 25-92% of an auxiliary agent in percentage by mass; the main agent is a mixture of trichoderma harzianum and difenoconazole.
Preferably, the mass percentage of the main agent is 46-66%, and the mass percentage of the auxiliary agent is 34-54%.
More preferably, the mass percentage of the main agent is 52-66%, and the mass percentage of the auxiliary agent is 34-48%.
Preferably, the auxiliary agent consists of a stabilizer, an auxiliary agent and a filler; according to the mass percentage, the stabilizer accounts for 0.5-4% of the bactericide, the auxiliary agent accounts for 12-40% of the bactericide, and the filler accounts for 12.5-48% of the bactericide. The auxiliary agent mainly plays a role in protecting conidia, enables the conidia to survive for a certain time in the compound sterilization preparation, and enables the conidia to be well dispersed on the surface of crops when in use.
Particularly preferably, the stabilizer is inorganic salt such as glycerol and/or phosphate; the auxiliary agent is one or a mixture of more than two of ascorbic acid, tween-80, sodium dodecyl sulfate, saponin, a wetting suspending agent DY6, sodium lignosulfonate and dinaphthylmethane disulfonate; the filler is one or a mixture of more than two of diatomite, kaolin or white carbon black.
In addition, the formulation of the microbial compound bactericide is also a common formulation in the field of pesticides, such as wettable powder, oil, direct fermentation liquor, aqueous solution, granular preparation, suspending agent, microcapsule bactericide and the like. Wettable powders are preferred.
The invention also provides application of the microbial compound bactericide in preventing and treating mulberry fruit diseases.
Experiments show that the microbial compound bactericide has a remarkable synergistic effect on prevention and treatment of diseases such as mulberry fruit rot and the like caused by pathogenic bacteria including sclerotinia sclerotiorum (sclerotinia sclerotiorum), mulberry cupara saghyna (Ciboria shirakana), caruncle cupara (Ciboria aruunchillides) and sclerotinia sclerotiorum (sclerotium shirana), so that in the application, the mulberry fruit rot is formed by the following components in percentage by weight: mulberry fruit disease caused by pathogenic bacteria such as Sclerotinia sclerotiorum (Sclerotinia sclerotiorum), Morus cassiterius (Ciboria shiriana), Ciboria carunculoides (Ciboria carunculoides) or Sclerotinia sclerotiorum (Sclerotinula shiriana).
Meanwhile, the application of the compound microorganism bactericide in the aspect of preventing and treating plant diseases caused by the pathogenic bacteria also belongs to the protection scope of the invention.
Compared with the prior art, the invention has the following beneficial effects:
1. according to long-term infection biological research and indoor bioassay, the Trichoderma harzianum strain Th-N5 has a strong infection bactericidal effect on various pathogenic bacteria diseases, the microbial compound bactericide obtained by compounding the Trichoderma harzianum strain Th-N5 with difenoconazole has a remarkable synergistic effect in preventing and treating mulberry fruit diseases, the average morbidity can be 4.6% at the lowest, the prevention and treatment effect is as high as 91%, and the prevention and treatment effect is remarkably superior to that of a single preparation; the dosage of the difenoconazole is reduced, and the problem of pesticide residue in the mulberry is solved; in addition, the microbial compound bactericide has good quick-acting property and persistence, can be absorbed by flowers of mulberries after 2 hours of pesticide application, can prevent new flowers from being damaged by germs, can obviously improve the quality and the yield of mulberries, and has a comprehensive effect obviously superior to other compound bactericides.
2. The strain Th-N5 used in the invention is obtained by separating naturally infected mulberry ginkgo in Guangzhou area of Guangdong province, is a native strain in China, is not introduced from abroad, and can be well adapted to local natural environment.
3. The microbial compound bactericide of the invention determines the optimal mixing proportion for obtaining good bactericidal effect, adapts to the requirements of organic food production, has no pollution and residue to the environment, and is beneficial to delaying the occurrence and development of the drug resistance of pathogenic bacteria.
Detailed Description
The invention is further illustrated by the following specific examples. The following examples are preferred embodiments of the present invention, but are not intended to limit the scope of the present invention in any manner. The present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention are deemed to be equivalent substitutions and should be included in the scope of the present invention.
Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated. Unless otherwise indicated, reagents and materials used in the following examples are commercially available.
The percentages stated in the examples below are in each case percentages by mass. The Trichoderma harzianum is a Trichoderma harzianum strain Th-N5, is separated from naturally infected mulberries and ginkgo in Guangzhou, is preserved in China center for type culture collection (CCTCC No): m2016251. Difenoconazole was purchased from the institute of plant protection, academy of agricultural sciences, guangdong province.
EXAMPLE 1 preparation of wettable powder of 50% Trichoderma harzianum and 2% Difenoconazole (diluted 2000 times when used)
The wettable powder comprises the following components in percentage by mass:
50% Trichoderma harzianum conidia powder (10)10(g), 2% of difenoconazole (technical product available in the market), 1% of glycerol, 2% of wetting and suspending agent DY6, 8% of tween-80, 4% of sodium lignosulfonate and 0.3% of ascorbic acid, wherein the diatomite is used for supplementing to 100%; wherein the mass ratio of the trichoderma harzianum conidium powder to the difenoconazole is 100: 4.
the bioassay results are shown in Table 1.
Example 2 preparation of wettable powder of 60% Trichoderma harzianum and 4% Difenoconazole (diluted 2000 times when in use)
The wettable powder comprises the following components in percentage by mass:
60% Trichoderma harzianum conidia powder (10)10/g) 4% of difenoconazole (technical product purchased in the market), 1% of glycerin, 5% of wetting and suspending agent DY6, 10% of tween-80, 6% of sodium lignosulfonate and 0.3% of ascorbic acid, wherein the amount of the sodium lignosulfonate is supplemented to 100% by Gaoling soil; wherein the mass ratio of the trichoderma harzianum conidium powder to the difenoconazole is about 100: 7.
The bioassay results are shown in Table 1.
Example 3 preparation of wettable powder of 60% Trichoderma harzianum and 6% Difenoconazole (diluted 2000 times when in use)
The wettable powder comprises the following components in percentage by mass:
60% Trichoderma harzianum conidia powder (10)10(g), 6% of difenoconazole (technical product available in the market), 1% of glycerol, 5% of wetting and suspending agent DY6, 10% of tween-80, 3% of sodium lignosulfonate and 0.3% of ascorbic acid, wherein kaolin is used for supplementing to 100%; wherein the mass ratio of the trichoderma harzianum conidium powder to the difenoconazole is 100: 10.
the bioassay results are shown in Table 1.
Example 4 preparation of wettable powder of 50% Trichoderma harzianum compounded with 1% Difenoconazole (diluted 2000 times when in use)
The wettable powder comprises the following components in percentage by mass:
50% Trichoderma harzianum conidia powder (10)10(g), 1% of difenoconazole (technical product available in the market), 1% of glycerol, 3% of wetting and suspending agent DY6, 8% of tween-80, 2% of sodium lignosulfonate and 0.3% of ascorbic acid, wherein the diatomite is used for supplementing to 100%; wherein the mass ratio of the trichoderma harzianum conidium powder to the difenoconazole is 100: 2.
example 5 preparation of wettable powder of 40% Trichoderma harzianum compounded with 6% Difenoconazole (diluted 2000 times when in use)
The wettable powder comprises the following components in percentage by mass:
40% Trichoderma harzianum conidium powder (10)10Per g), 6% difenoconazole (original commercial product)Medicine), 0.5% of glycerin, 2% of wetting suspending agent DY6, 7% of Tween-80, 6% of sodium lignosulfonate, and 0.3% of ascorbic acid, which are supplemented to 100% by Gaoling soil; wherein the mass ratio of the trichoderma harzianum conidium powder to the difenoconazole is about 100: 15.
Example 6 prevention and treatment effects of the compounded wettable powder on mulberry white fruit diseases and determination of yield increase effects of mulberries
1. Preparation of test strain spore suspension and difenoconazole solution
Inoculating purified Trichoderma harzianum strain Th-N5 on PDA plate, culturing in thermostatic oven (14L: 10D) at 25 + -1 deg.C for 5-7 days, adding sterile water of 0.1% Tween-80 to collect spores, stirring the spore suspension on magnetic stirrer, dispersing the spores uniformly, filtering with medical gauze and mirror paper to remove impurities to obtain spore suspension, counting with blood cell counting plate to determine spore concentration, and making into 3 × 106Spore/ml of spore solution for use.
The difenoconazole is prepared into solutions with the concentrations of 0.1, 0.2 and 0.3mg/L for standby (the difenoconazole is converted into the content of the original drug).
The wettable powders prepared in example 1, example 2 and example 3 were diluted to 2000-fold liquid as required for use.
2. Determination of prevention and treatment effects of Trichoderma harzianum strain Th-N5, difenoconazole and compounded wettable powder on mulberry fruit rot
Selecting 2-year-old potted mulberry with consistent growth and management levels as an experimental object, randomly dividing 72 mulberries into 24 cells, wherein each cell comprises 3 mulberries and a protective zone of 3-5 m is arranged around each cell.
The test is provided with 8 treatment groups, which are respectively as follows:
(1)0 (clear water);
(2) 0.1mg/L, 0.2mg/L, 0.3mg/L and 600mg/L (800 times dilution is recommended in the market, and the concentration is reduced to the original drug concentration) of difenoconazole respectively and independently;
(3) 3 x 10 alone6Spores/ml of trichoderma harzianum spore suspension;
(4) examples after dilution1 wettable powder (containing 0.1mg/L difenoconazole and 3 x 10)6spores/mL trichoderma harzianum spores);
(5) diluted wettable powder of example 2 (containing 0.2mg/L difenoconazole + 3X 10)6Spores/ml trichoderma harzianum spores);
(6) diluted wettable powder of example 3 (containing 0.3mg/L difenoconazole + 3X 10)6Spores/ml trichoderma harzianum spores).
Each process was repeated 3 times for 24 cells.
Spraying the pesticide by each cell according to a conventional method in the flowering phase of the mulberry, and spraying the pesticide on flowers and nearby branches of the mulberry according to a scheme in the same way in 2017 for 3, 5, 10, 15, 20, 25, 30, 5, 10, 4, 10 and 15 months. The air temperature is 7-23 ℃ during the pesticide application period, no rain exists in the spraying day, and the pesticide is sprayed again after the spraying is stopped if the spraying is rainy. And (3) investigating mulberry white fruit diseases at 5, 10, 15 and 25 days in 5 months, randomly investigating 3 branches per tree, recording the total mulberry fruit number and the diseased fruit number on the branches, and calculating the control effect, wherein the results are shown in table 1.
Control effect (%) [ (control group incidence-incidence of treatment group)/control group incidence ] × 100%.
3. Influence of Trichoderma harzianum strain Th-N5, difenoconazole and compounded wettable powder on yield of mulberry
The mulberry yield is checked 5, 10, 15, 25 and 30 days after each plot is subjected to pesticide application treatment, mature fruits on fruit trees are picked, diseased fruits are removed, then weighing is carried out, and the yield increasing effect of the mulberries in different treatment areas is calculated, wherein the results are shown in table 1.
The yield increase effect (%) is [ (total yield of the mulberry in the treatment group-total yield of the mulberry in the control group)/total yield of the mulberry in the control group ] × 100%.
All the test data are processed and completed on a data processing software SAS system.
TABLE 1 comparison of control effects on white fruit of Mori fructus in different treatment areas
Note: in the table, the same letters after the same numbers indicate that the difference was not significant (DMRT method).
As shown in table 1, the experimental results show that the incidence rates of the mulberry fruit diseases in the treatment area using the compound agent are both lower than 13%, and are significantly lower than the incidence rates of the mulberry fruit diseases in the treatment area using a single agent and the control area using a single agent; the prevention and treatment effects of the compound agent on the white fruit diseases of the mulberries are higher than 75 percent and can reach 91 percent at most, and the prevention and treatment effects are obviously higher than those of a single agent on the white fruit diseases; and the yield of the mulberries in the compound agent treatment area is increased by over 35 percent, the maximum yield can be increased by 47.5 percent, and the yield is obviously higher than that of the mulberries in the single agent treatment area. Meanwhile, the results also show that the compound use of the trichoderma harzianum and the difenoconazole can obviously reduce the using amount of the difenoconazole, and meanwhile, the prevention and treatment effect and the yield increasing effect are obviously improved, and the morbidity is obviously reduced.
4. Determination of content of difenoconazole in mulberry flowers and mulberries after trichoderma harzianum strain Th-N5, difenoconazole and compounded wettable powder are used
The samples to be detected were mulberry flowers and ripe mulberry fruits 2, 3, 4 hours and 5, 6 days after application. The sample processing method and the sample detection method refer to Liu Hao et al (Chinese traditional medicine journal, 2009, 33 (18): 2096-2098). The method specifically comprises the following steps: putting 20g of a sample into a 1000mL triangular flask, adding 300mL of acetonitrile, soaking overnight, performing ultrasonic extraction for 30min by 2 times with 400mL of acetonitrile, filtering, and washing the triangular flask and filter residues; concentrating at 40 deg.C under low pressure to 5mL, and naturally drying; a glass chromatographic column tube is taken, a small amount of absorbent cotton, anhydrous sodium sulfate with the thickness of 2cm, 5g of neutral alumina and anhydrous sodium sulfate with the thickness of 2cm are sequentially filled in the glass chromatographic column tube, a pretreatment column is prepared, and petroleum ether is used for treatment before use. Dissolving the extracted sample with petroleum ether-diethyl ether, transferring to a pretreatment column for purification, eluting with a petroleum ether-diethyl ether mixed solution, collecting an eluent, metering the volume of the concentrated and nearly dried sample with petroleum ether, and determining the content of difenoconazole in the sample by gas chromatography. The gas chromatography was carried out using an Agilent5890N Network GC System (Agilent, USA), HP-5 capillary column (30 m.times.0.32 mm.times.0.25 μm), flow rate of 1.2mL/min, and vaporizer temperature of 260 ℃.
The detection results show that in mulberry flower samples 2, 3 and 4 hours and 5 and 6 days after application, except that difenoconazole can not be detected in a clear water group, the difenoconazole can be detected in the other eight groups of treatment areas. However, in mature mulberries, the Trichoderma harzianum strain Th-N5, the formulated wettable powder treatment group, the low-dose difenoconazole treatment group and the clear water control group could not detect difenoconazole, while the high-dose difenoconazole treatment group (600 mg/mL) could detect difenoconazole residue. The difenoconazole can be absorbed by the mulberry flowers 2 hours after the application, the difenoconazole still remains in the mulberry flowers 6 days after the application, and the difenoconazole continuously exists in the mulberry flowers when the next application is carried out, so that the difenoconazole can be continuously prevented and treated, and the action time is effectively shortened. Meanwhile, the trichoderma harzianum strain Th-N5 and the low-dose difenoconazole are compounded for use, so that pesticide residue in the mature mulberry cannot be caused, and the quality of the mulberry can be improved.
In conclusion, by comparing and analyzing data of preventing and treating the mulberry fruit disease by using a large amount of biological pesticide mixture, the invention screens out substances and proportions which can obviously improve the preventing and treating effect of the mulberry fruit disease so as to effectively kill pathogenic bacteria infecting plants in a short time and enhance the preventing and treating effect. On the basis, the best combination of substances for promoting the prevention and treatment effect of the mulberry and the ginkgo is obtained through a large number of tests and explorations, and the prevention and treatment effect is verified through experiments. Experimental results prove that the microbial compound bactericide can obviously improve the control effect of the mulberry fruit disease, can obviously improve the yield of the mulberry fruit, effectively shortens the action time of the compound agent, and has good quick action and persistence.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, and simplifications are intended to be included in the scope of the present invention.
Claims (6)
1. The microbial compound bactericide for preventing and treating the mulberry fruit disease is characterized by comprising 8-75% of a main agent and 25-92% of an auxiliary agent by mass percent; the main agent is a mixture of trichoderma harzianum and difenoconazole, and the mass ratio of the trichoderma harzianum to the difenoconazole is 100: 4. 100, and (2) a step of: 7 or 100: 10; the trichoderma harzianum is trichoderma harzianum strain Th-N5, is preserved in China Center for Type Culture Collection (CCTCC) at 2016, 5 and 6, and has a preservation number of CCTCC No: m2016251;
the auxiliary agent consists of a stabilizer, an auxiliary agent and a filler, wherein the stabilizer accounts for 0.5-4% of the bactericide, the auxiliary agent accounts for 12-40% of the bactericide, and the filler accounts for 12.5-48% of the bactericide by mass percent.
2. The microbial compound bactericide as claimed in claim 1, wherein the trichoderma harzianum is conidium powder of trichoderma harzianum.
3. The microbial compound bactericide as claimed in claim 2, wherein the stabilizer is glycerol and/or an inorganic salt; the auxiliary agent is one or a mixture of more than two of ascorbic acid, tween-80, sodium dodecyl sulfate, saponin, a wetting suspending agent DY6, sodium lignosulfonate and dinaphthyl methane disulfonate; the filler is one or a mixture of more than two of diatomite, kaolin or white carbon black.
4. The microbial compound bactericide as claimed in any one of claims 1 to 3, wherein the microbial compound bactericide is a wettable powder.
5. The application of the microbial compound bactericide of any one of claims 1-3 in preventing and treating mulberry fruit diseases.
6. The application of the microbial compound bactericide in the aspect of controlling plant diseases caused by pathogenic bacteria according to any one of claims 1 to 3, wherein the pathogenic bacteria are Sclerotinia sclerotiorum (sclerotiorum), cupara multocida (ciboriashiariana), cupara carunculus (Ciboria carunculoides) and/or sclerotium vulgare (sclerotium monilifloraiana).
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