CN107586728A - A kind of cordyceps sinensis nutrient solution and preparation method thereof - Google Patents
A kind of cordyceps sinensis nutrient solution and preparation method thereof Download PDFInfo
- Publication number
- CN107586728A CN107586728A CN201711016779.6A CN201711016779A CN107586728A CN 107586728 A CN107586728 A CN 107586728A CN 201711016779 A CN201711016779 A CN 201711016779A CN 107586728 A CN107586728 A CN 107586728A
- Authority
- CN
- China
- Prior art keywords
- nutrient solution
- cordyceps sinensis
- liquid
- zymotic fluid
- liquefaction
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to technical field of fungal fermentation, more particularly to a kind of cordyceps sinensis nutrient solution, the nutrient solution contains the zymotic fluid after separation mycelia, zymotic fluid after separation mycelia is reclaimed in proportion and returns Foundation liquefaction feed liquid as the preparation raw material of new nutrient solution, in addition, slag charge can be diluted proportioning and squeeze to obtain extracting juice, return back Foundation liquefaction feed liquid, carry out follow-up dispensing operation.Therefore, nutrient solution provided by the invention is prepared using being equipped with dispensing by the high efficiente callback of zymotic fluid and juicing, and the use to save material is final to reduce the fermentation cost of raw material, and then reduces total cost of production, improves added value of product.
Description
Technical field
The present invention relates to technical field of fungal fermentation, and in particular to a kind of cordyceps sinensis nutrient solution and preparation method thereof.
Background technology
Cordyceps sinensis is for a long time pharmaceutically acceptable and edible, is a kind of high-grade invigorant, its property of medicine is gentle, than other kinds
The invigorant of class has a wider medicinal and edibility, and all edible throughout the year, to old or young, sick, weak, virtual person
All preferably take, have no side effect.In recent years, cordyceps sinensis demand is increased severely both at home and abroad, but because cordyceps sinensis has its special life
State environment and strict parasitics, yield is fairly limited, resource scarcity.To alleviate the imbalance between supply and demand in market, the people of cordyceps sinensis
Work fermented product is more and more important, is to solve the medicinal money of cordyceps sinensis using liquid deep layer fermenting culture aweto mycelium
A kind of source effective way in short supply.Research shows that artificial fermentation cultivates obtained Chinese caterpillar fungus bacterial filament, through toxicity, pharmacology, plant
Research, it was demonstrated that it is basically identical with natural cordyceps chemical composition, pharmacological action, natural cordyceps production cordyceps sinensis system can be replaced
Product, to make up the shortage of natural resources.
Cordyceps fermented liquid is to pass through liquid fermentation and culture by Hirsutella sinensis strain, separates the fermentation obtained after mycelia
Liquid.At present in fermenting and producing：(1) the zymotic fluid producing level after extraction mycelia is limited, and most zymotic fluids are as waste material
Sewage disposal system is directly entered, not only causes the wasting of resources, the pollution to environment is also than more serious；(2) meeting after raw material liquefaction
Slag charge is produced, these slag charges also directly as waste disposal, cause the wasting of resources.Therefore, in prior art industrial production
Waste material (zymotic fluid and liquefaction slag charge) recycles in the present invention, for preparing new culture medium, reduces the wasting of resources, saves
Production raw material.
In the prior art, the used medium that fermented when producing Chinese caterpillar fungus bacterium powder is all ordinary culture medium, but will separation
Zymotic fluid after mycelia recycles and liquefaction feed liquid prepares culture medium again together, the training new as fermentation Chinese caterpillar fungus bacterium powder
The technology for supporting base not yet reports that therefore, the present invention reclaims the zymotic fluid after separating mycelia and liquefaction slag charge according to a certain percentage
By the use of as fermentation raw material, reduce the consumption of raw material.
The content of the invention
The purpose of the present invention is in view of the above-mentioned problems, the defects of in order to overcome prior art, there is provided one kind can effectively improve
The cordyceps sinensis nutrient solution of Chinese caterpillar fungus bacterium powder production utilization rate of raw materials, it is expected based on the supernatant after liquefaction, is added
Enter to liquefy and feed liquid and separate the zymotic fluid after mycelia, be equipped with auxiliary material and be prepared again, rationally and make full use of fermentation raw material,
Final realize reduces the fermentation cost of raw material, and then reduces total cost of production.
The present invention is that technical scheme is used by solving technical problem：
A kind of cordyceps sinensis nutrient solution, its feature are：The nutrient solution contains the fermentation after separation Chinese caterpillar fungus bacterial filament
Liquid, the zymotic fluid are obtained by procedure below：
(1) nutrient solution is prepared：Corn and silkworm chrysalis are crushed respectively, sieved, takes 40~80 mesh corn flour and dried silkworm chrysalis meal standby；
1~3wt% corn flour, 1~3wt% dried silkworm chrysalis meal and 0.2~0.4wt% protein water is put into liquefied pot
Enzyme is solved, supplies water, stops heating when being heated to 100~105 DEG C, Temperature fall 4~8 hours, obtains the feed liquid that liquefies, point
From collecting supernatant；
Glucose sugar, peptone and yeast extract are added in the supernatant collected, make the content of reduced sugar in mixed liquor for 15~
30g/L, amino nitrogen content are 0.5~0.8g/L, adjust pH to 7~7.5, obtain nutrient solution A；
(2) sterilize：Sterilization treatment is carried out to nutrient solution A；
(3) ferment：Hirsutella sinensis strain is inoculated in fermented and cultured in the nutrient solution A after sterilizing；
(4) separate：After fermentation ends, separation of solid and liquid, mycelium and zymotic fluid is separately recovered.
Further, Stress control is in 0.11~0.15MPa during the step (2) sterilizing, and temperature control is 121~125
DEG C, sterilize 30~60min.
Further, Hirsutella sinensis strain is expanded into culture step by step during step (3) fermentation, treats cordyceps sinensis in tank
Mycelia bulk concentration terminates to ferment when reaching 30~50%.
Further, the nutrient solution is further containing liquefaction feed liquid, and liquefaction feed liquid includes liquefaction supernatant and liquefaction is squeezed
Take liquid.
Meanwhile the present invention provides a kind of preparation method of cordyceps sinensis nutrient solution, the nutrient solution is obtained by following steps：
(1) raw material crushes：Corn and silkworm chrysalis are crushed respectively, sieved, takes 40~80 mesh corn flour and dried silkworm chrysalis meal standby；
(2) liquefy：1~3wt% corn flour, 1~3wt% dried silkworm chrysalis meal and 0.2~0.4wt% are put into liquefied pot
Proteolytic enzyme, water is supplied, stop heating when being heated to 100~105 DEG C, Temperature fall 4~8 hours, liquefied
Feed liquid；
(3) separate：Liquefaction feed liquid is extracted into supernatant；Slag charge squeezes, and juicing and squeezing slag charge is separately recovered；
(4) prepare：By 20~50wt% Cordyceps fermented liquid, 40%~60wt% supernatants and 10%~20w%
Juicing mixes, and adds auxiliary material, the content for making reduced sugar in mixed liquor is 15~30g/L, and amino nitrogen content is 0.5~0.8g/
L, pH to 7~7.5 is adjusted, obtains nutrient solution B.
Further, squeezed after the water mixing of 0.5~2 times of slag charge is added when slag charge squeezes in the step (3).
Further, the auxiliary material in the step (4) is glucose sugar, peptone and yeast extract, and these auxiliary materials are directly from city
Field purchase use.
Compared with prior art, a kind of cordyceps sinensis nutrient solution provided by the invention, it has the following effects that：
(1) caused slag charge will liquefy according to the criterion of arm's length basis liquefaction feed liquid after the present invention liquefies raw material
Slag charge is diluted proportioning, returns back Foundation liquefaction feed liquid, as the preparation raw material of new nutrient solution, has saved liquefaction raw material；
(2) zymotic fluid after separation mycelia reclaims in proportion returns back liquefaction feed liquid, it is determined that recovery ratio and rear charge ratio
Preparation raw material of the example as nutrient solution, for fermenting and producing next time, reduce raw materials consumption and trash discharge；
(3) using nutrient solution provided by the present invention production Chinese caterpillar fungus bacterium powder, liquefied bulk material (corn and silkworm before optimization
Pupa) compared with 50% or so can be saved after optimizing, total raw material averagely saves 20~25% or so in overall preparation.
Brief description of the drawings
Fig. 1 is the process chart that the present invention prepares Cordyceps fermented liquid.
Fig. 2 is a kind of preparation technology flow chart of cordyceps sinensis nutrient solution of the present invention.
Embodiment
To make those skilled in the art more fully understand technical scheme, enter with reference to the accompanying drawings and examples
One step illustrates the present invention.It should be understood that these embodiments are only illustrative of the invention and is not intended to limit the scope of the invention.It is following
The experimental method of unreceipted actual conditions in embodiment, generally according to normal condition.
Unless otherwise defined, anticipated known to all specialties used in text and scientific words and one skilled in the art
Justice is identical.In addition, any method similar or impartial to described content and material all can be applied in the inventive method, Wen Zhong
Described preferable implementation and material only present a demonstration effect.
Embodiment 1
The present embodiment provides a kind of cordyceps sinensis nutrient solution, and the nutrient solution contains the zymotic fluid after separation mycelia, wherein, should
Zymotic fluid is obtained by procedure below：
(1) nutrient solution is prepared：Corn and silkworm chrysalis are crushed respectively, sieved, takes 40 mesh corn flour and dried silkworm chrysalis meal standby；
1wt% corn flour, 3wt% dried silkworm chrysalis meal and 0.2wt% proteolytic enzyme is put into liquefied pot, remaining is
Water, stop heating when being heated to 100 DEG C, Temperature fall 4 hours, obtain the feed liquid that liquefies, separation, collect supernatant；
Glucose sugar, peptone and yeast extract are added in the supernatant for needing to prepare, makes the content of reduced sugar in mixed liquor
For 15~30g/L, amino nitrogen content is 0.5~0.8g/L, adjusts pH to 7~7.5, obtains nutrient solution A；
(2) sterilize：Nutrient solution A is sterilized 30min under the conditions of 0.11MPa, 121 DEG C；
(3) ferment：Hirsutella sinensis strain is inoculated in the nutrient solution A after sterilizing and expands culture step by step, treats the winter in tank
Worm summer grass mycelia bulk concentration terminates to ferment when reaching 30%；
(4) separate：After fermentation ends, mycelium and zymotic fluid is separately recovered in separation of solid and liquid, and zymotic fluid recycles.
Meanwhile also containing liquefaction feed liquid in the nutrient solution, liquefaction feed liquid includes liquefaction supernatant and liquefaction slag takes liquid, is
This, the present invention provides a kind of preparation method of cordyceps sinensis nutrient solution, and the nutrient solution is obtained by following steps：
(1) raw material crushes：Corn and silkworm chrysalis are crushed respectively, sieved, takes 40 mesh corn flour and dried silkworm chrysalis meal standby；
(2) liquefy：The protein hydrolysis of 1wt% corn flour, 3wt% dried silkworm chrysalis meal and 0.2wt% is put into liquefied pot
Enzyme, remaining is water, stops heating when being heated to 100 DEG C, Temperature fall 4 hours, obtains the feed liquid that liquefies.
(3) separate：Liquefaction feed liquid is collected into supernatant；Slag charge is squeezed, and the water of 0.5 times of slag charge weight is added during squeezing, is mixed
Combined pressure is squeezed, and juicing and squeezing slag charge is separately recovered；
(4) prepare：20wt% Cordyceps fermented liquid, 60wt% supernatants and 20w% juicings are mixed, add Portugal
Sugared sugar, peptone and yeast extract, the content for making reduced sugar in mixed liquor be 30g/L, amino nitrogen content 0.8g/L, adjust pH to
7.5, obtain nutrient solution B.
Embodiment 2
The present embodiment provides a kind of cordyceps sinensis nutrient solution, and the nutrient solution contains the zymotic fluid after separation mycelia, wherein, should
Zymotic fluid is obtained by procedure below：
(1) nutrient solution is prepared：Corn and silkworm chrysalis are crushed respectively, sieved, takes 70 mesh corn flour and dried silkworm chrysalis meal standby；
2wt% corn flour, 2wt% dried silkworm chrysalis meal and 0.3wt% proteolytic enzyme is put into liquefied pot, remaining is
Water, stop heating when being heated to 103 DEG C, Temperature fall 6 hours, obtain the feed liquid that liquefies, separation, collect supernatant；
Glucose sugar, peptone and yeast extract are added in the supernatant for needing to prepare, makes the content of reduced sugar in mixed liquor
For 25g/L, amino nitrogen content 0.6g/L, pH to 7.5 is adjusted, obtains nutrient solution A；
(2) sterilize：Nutrient solution A is sterilized 45min under the conditions of 0.13MPa, 123 DEG C；
(3) ferment：Hirsutella sinensis strain is inoculated in the nutrient solution A after sterilizing and expands culture step by step, treats the winter in tank
Worm summer grass mycelia bulk concentration terminates to ferment when reaching 40%；
(4) separate：After fermentation ends, mycelium and zymotic fluid is separately recovered in separation of solid and liquid, and zymotic fluid recycles.
Meanwhile also include liquefaction supernatant and liquefaction containing liquefaction feed liquid, liquefaction feed liquid in nutrient solution provided by the invention
Slag takes liquid, therefore, the present embodiment provides a kind of preparation method of cordyceps sinensis nutrient solution, the nutrient solution is obtained by following steps：
(1) raw material crushes：Corn and silkworm chrysalis are crushed respectively, sieved, takes 70 mesh corn flour and dried silkworm chrysalis meal standby；
(2) liquefy：The protein hydrolysis of 2wt% corn flour, 2wt% dried silkworm chrysalis meal and 0.3wt% is put into liquefied pot
Enzyme, remaining is water, stops heating when being heated to 103 DEG C, Temperature fall 6 hours, obtains the feed liquid that liquefies.
(3) separate：Liquefaction feed liquid is collected into supernatant；Slag charge is squeezed, and the water with slag charge equivalent, mixing pressure are added during squeezing
Squeeze, juicing and squeezing slag charge is separately recovered；
(4) prepare：35wt% Cordyceps fermented liquid, 45wt% supernatants and 15w% juicings are mixed, add Portugal
Sugared sugar, peptone and yeast extract, the content for making reduced sugar in mixed liquor be 25g/L, amino nitrogen content 0.6g/L, adjust pH to
7.5, obtain nutrient solution B.
Embodiment 3
The present embodiment provides a kind of cordyceps sinensis nutrient solution, and the nutrient solution contains the zymotic fluid after separation mycelia, wherein, should
Zymotic fluid is obtained by procedure below：
(1) nutrient solution is prepared：Corn and silkworm chrysalis are crushed respectively, sieved, takes 70 mesh corn flour and dried silkworm chrysalis meal standby；
2wt% corn flour, 2wt% dried silkworm chrysalis meal and 0.3wt% proteolytic enzyme is put into liquefied pot, remaining is
Water, stop heating when being heated to 103 DEG C, Temperature fall 6 hours, obtain the feed liquid that liquefies, separation, collect supernatant；
Glucose sugar, peptone and yeast extract are added in the supernatant for needing to prepare, makes the content of reduced sugar in mixed liquor
For 25g/L, amino nitrogen content 0.6g/L, pH to 7 is adjusted, obtains nutrient solution A；
(2) sterilize：Nutrient solution A is sterilized 60min under the conditions of 0.15MPa, 125 DEG C；
(3) ferment：Hirsutella sinensis strain is inoculated in the nutrient solution A after sterilizing and expands culture step by step, treats the winter in tank
Worm summer grass mycelia bulk concentration terminates to ferment when reaching 50%；
(4) separate：After fermentation ends, mycelium and zymotic fluid is separately recovered in separation of solid and liquid, and zymotic fluid recycles.
Meanwhile also containing liquefaction feed liquid in the nutrient solution, liquefaction feed liquid includes liquefaction supernatant and liquefaction slag takes liquid, is
This, the present embodiment provides a kind of preparation method of cordyceps sinensis nutrient solution, and the nutrient solution is obtained by following steps：
(1) raw material crushes：Corn and silkworm chrysalis are crushed respectively, sieved, takes 80 mesh corn flour and dried silkworm chrysalis meal standby；
(2) liquefy：The protein hydrolysis of 3wt% corn flour, 1wt% dried silkworm chrysalis meal and 0.4wt% is put into liquefied pot
Enzyme, remaining is water, stops heating when being heated to 105 DEG C, Temperature fall 8 hours, obtains the feed liquid that liquefies.
(3) separate：Liquefaction feed liquid is collected into supernatant；Slag charge is squeezed, and the water of 2 times of slag charge weight, mixing are added during squeezing
Squeezing, juicing and squeezing slag charge is separately recovered；
(4) prepare：50wt% Cordyceps fermented liquid, 40wt% supernatants and 10w% juicings are mixed, add Portugal
Sugared sugar, peptone and yeast extract, the content for making reduced sugar in mixed liquor be 15g/L, amino nitrogen content 0.5g/L, adjust pH to
7, obtain nutrient solution B.
Embodiment 4：The material residue that liquefies material dilution proportioning test
From 70 mesh screened materials, with 0.3% proteolytic enzyme K processing, the liquid medium material of progress 10 batches
Experimental data is collected, and soup stock dry weight, ammonia nitrogen, reduced sugar is detected, and carry out 1 with running water:1 dilution, after dilution
Detection identical data, dry weight, ammonia nitrogen and the content of reducing sugar of ten batches of liquefaction soup stocks of METHOD FOR CONTINUOUS DETERMINATION, data are as follows：
Table 1 liquefy material residue material in nutriment dissolution rate and dilution 1 times after detection data
The liquefaction material of the slag charge of table 2 dilution and former clear liquid liquefaction material shaking flask yield comparison experiment (average value three times)
The Chinese caterpillar fungus bacterial filament yield and production no significant difference at present of culture are expected in two kinds of liquefaction of Experimental comparison, and after culture
It is qualified that the bacterium powder of acquisition detects main physical and chemical index according to standards of pharmacopoeia.
As a result show, be mostly to complete liquefied liquid material in the material residue material that liquefies, technological transformation can be passed through completely
Either isolation technics improvement and recycle.About 1 times is higher by by the more former clear liquid liquefaction material of liquefaction material concentration after recovery
Left and right, 1 can be used：1 ratio is diluted, and culture experiment confirms after dilution, this material and former clear liquid liquefaction material culture effect
Unanimously, product quality and yield are had no adverse effects.
Embodiment 5：The zymotic fluid that mycelia is separated after fermentation returns recycling
There is the nutriment not being fully used in zymotic fluid in view of separating mycelia after fermentation, try as again
The culture raw material of secondary fermentation, the carbon source and nitrogen source suitably matched is added, strain is accessed after high-temperature sterilization, measures and is testing
Under the conditions of mycelia growing state and mycelia yield how, as in fermenting and producing from now on zymotic fluid recycle experiment according to
According to.
Using the residual quantity of carbon nitrogen source in the nutrient solution of normal extraction as reference, different amounts of carbon source is added in follow-up dispensing
And nitrogen source, Three factors-levels orthogonal test is designed, investigates the dosage in the subsequent material of zymotic fluid refund part.
Table 3 returns material charge ratio experimental design table
Experiment 500mL triangular flasks, loading amount 200ml, inoculum concentration 10%, each group nutrient solution dry weight, reduction before detection inoculation
Sugar and amino nitrogen content.Every group in orthogonal test is done two repetitions, is put to sterile small shaking table culture.Culture was to 10~12 days
When put bottle, detect the dry weight of mycelia and ammonia nitrogen and content of reducing sugar in nutrient solution in each triangular flask.
The every detection data of each experimental group before and after the inoculated and cultured of table 4
The zymotic fluid of table 5 returns test result analysis after the carbon source nitrogen source inoculated and cultured for expecting addition different proportion
Note：A is glucose, and B is peptone, and C is yeast extract；T is the average of factorial experiments result sum；
R is maximum number-minimum number in t values
Using dry mycelial weight as judgment standard it can be seen from the analyze data in upper table, the addition of glucose is to dry weight
Influence be maximum, with the increase of glucose addition, dry mycelial weight is significantly increased, illustrate glucose be influence mycelia
The increased principal element of dry weight, the addition of glucose turn into the most important restraining factors of dry mycelial weight lifting, therefore grape
The addition of sugar also needs to be lifted；The addition of peptone also has considerable influence to dry mycelial weight, and the influence of yeast extract is relatively small.
The check experiment of different glucose additions and normal production technology formula, testing program and the results are shown in Table shown in 6：
Table 6 normally follow-up dosage and return material different proportion dosage scheme and result
Experiment conclusion：Dry mycelial weight between the control experiment group of actual production still differs bigger, therefore, completely
Though having feasibility by the use of the zymotic fluid of fermentation termination as new culture, the color of mycelia is partially deep, and finished product color and luster is had a great influence,
It is feasible not strong in actual production.
In addition, the data of dry weight can be seen that glucose addition to dry mycelial weight influence maximum from table, with grape
The increase of sugared addition, dry mycelial weight are significantly increased, and it is to influence the increased principal element of dry mycelial weight, grape to illustrate glucose
The addition of sugar also needs to be lifted.
Embodiment 6：Zymotic fluid returns proportioning test
In view of directly doing cultural hypha with the zymotic fluid of recovery, during fermentation termination the yield of mycelia it is few compared with normal control and
Mycelia color and luster is partially deep, this time we by zymotic fluid by different proportion be added to liquefaction material in, then add in proportion other nutrition into
Cultivated after point, the addition of glucose is consistent with the addition in production at present.
Using normal fermentation production technology formula as control, the and of design recovery zymotic fluid addition 20%, 30%, 50%
100% different tests group carries out contrast test, and each group of formula of testing see the table below：
The zymotic fluid of table 7 returns Ratio Experiments arrangement
In addition to the liquefaction material of use returns material with fresh liquid material or zymotic fluid, remaining dispensing is performed by original technique.Often
Bottled amount 220ml, inoculum concentration 20ml, each group nutrient solution dry weight, reduced sugar and amino nitrogen content before detection inoculation.Each test group
Two repetitions are done, are put to sterile small shaking table culture.The Duplicate Samples taken respectively during to 10,11 days in each test group are cultivated,
Detect the dry weight of mycelia and ammonia nitrogen and content of reducing sugar in nutrient solution in each triangular flask.
The different fermentations liquid of table 8 returns the every detection data of each experimental group before and after ratio inoculation
As a result show, a certain amount of zymotic fluid, the dry weight of mycelia and the dry mycelial weight phase of control group are added in liquefaction is expected
When, it was demonstrated that it is feasible that zymotic fluid part, which is returned as nutrient solution,.
Embodiment 7：The determination test of conservation of raw material rate
Contrast test：So that production configures 1 ton of cordyceps sinensis fermentation culture as an example
The conservation of raw material rate contrast test of table 9
Exemplified by producing 1 ton of nutrient solution：The manufacturing process of complete nutrient solution is part raw material liquefaction hydrolysis, and what is obtained is clear
Liquid carries out compounding surplus stock.
1 ton of liquefaction material composition is as follows, and in the feed liquid manufacturing process that liquefies, the 50% of inventory is fed intake according to schedule,
Produce 0.5 ton of fresh liquid material, the juicing for squeezing centrifugation dilution of 20% (0.2 ton) of increase and the hair of 30% (0.3 ton)
Zymotic fluid reclaimed materials, new liquefaction material clear liquid is formed, follow-up compounding raw material does not change, equivalent in liquefaction feed liquid production
During save 50% or so raw material input；Other compounding raw materials are added in preparation, therefore, the original of liquefaction material part
Material reduces 23.9% or so in total zymotic fluid production cost.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in,
It should all be included within the scope of the present invention.
Claims (9)
- A kind of 1. cordyceps sinensis nutrient solution, it is characterised in that：The nutrient solution contains the zymotic fluid after separation Chinese caterpillar fungus bacterial filament.
- 2. a kind of cordyceps sinensis nutrient solution according to claim 1, it is characterised in that the zymotic fluid is obtained by following steps Arrive：(1) nutrient solution is prepared：Corn and silkworm chrysalis are crushed respectively, sieved, takes 40~80 mesh corn flour and dried silkworm chrysalis meal standby；The protein hydrolysis of 1~3wt% corn flour, 1~3wt% dried silkworm chrysalis meal and 0.2~0.4wt% is put into liquefied pot Enzyme, water is supplied, stops heating when being heated to 100~105 DEG C, Temperature fall 4~8 hours, obtain the feed liquid that liquefies, separate, Collect supernatant；Add glucose sugar, peptone and yeast extract in supernatant, the content for making reduced sugar in mixed liquor is 15~30g/L, ammonia Base nitrogen content is 0.5~0.8g/L, adjusts pH to 7~7.5, obtains nutrient solution A；(2) sterilize：Sterilization treatment is carried out to nutrient solution A；(3) ferment：Hirsutella sinensis strain is inoculated in fermented and cultured in the nutrient solution A after sterilizing；(4) separate：After fermentation ends, separation of solid and liquid, mycelium and zymotic fluid is separately recovered.
- A kind of 3. cordyceps sinensis nutrient solution according to claim 2, it is characterised in that：During step (3) fermentation by State's hair spore strain expands culture step by step, terminates to ferment when aweto mycelium concentration reaches 30~50%.
- A kind of 4. cordyceps sinensis nutrient solution according to claim 1, it is characterised in that：Pressure during step (2) sterilizing Control is in 0.11~0.15MPa, and temperature control is at 121~125 DEG C, and sterilize 30~60min.
- A kind of 5. cordyceps sinensis nutrient solution according to claim 1, it is characterised in that：The nutrient solution further contains liquid Material liquid.
- A kind of 6. cordyceps sinensis nutrient solution according to claim 5, it is characterised in that：The liquefaction feed liquid is included in liquefaction Clear liquid and liquefaction juicing.
- 7. the preparation method of a kind of cordyceps sinensis nutrient solution according to claim 6, it is characterised in that obtained by following steps Arrive：(1) raw material crushes：Corn and silkworm chrysalis are crushed respectively, sieved, takes 40~80 mesh corn flour and dried silkworm chrysalis meal standby；(2) liquefy：1~3wt% corn flour, 1~3wt% dried silkworm chrysalis meal and 0.2~0.4wt% albumen is put into liquefied pot Matter hydrolase, supplies water, stops heating when being heated to 100~105 DEG C, Temperature fall 4~8 hours, obtains the feed liquid that liquefies；(3) separate：Extract supernatant；Slag charge squeezes, and juicing and squeezing slag charge is separately recovered；(4) prepare：20~50wt% Cordyceps fermented liquid, 40%~60wt% supernatants and 10%~20w% are squeezed Liquid mixes, and adds auxiliary material, and the content for making reduced sugar in mixed liquor is 15~30g/L, and amino nitrogen content is 0.5~0.8g/L, adjusts PH to 7~7.5 is saved, obtains nutrient solution B.
- A kind of 8. preparation method of cordyceps sinensis nutrient solution according to claim 7, it is characterised in that：The step (3) Squeezed after the water mixing of 0.5~2 times of slag charge is added during middle slag charge squeezing.
- A kind of 9. preparation method of cordyceps sinensis nutrient solution according to claim 7, it is characterised in that the step (4) In auxiliary material be glucose sugar, peptone and yeast extract.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711016779.6A CN107586728B (en) | 2017-10-26 | 2017-10-26 | Cordyceps sinensis culture solution and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711016779.6A CN107586728B (en) | 2017-10-26 | 2017-10-26 | Cordyceps sinensis culture solution and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107586728A true CN107586728A (en) | 2018-01-16 |
| CN107586728B CN107586728B (en) | 2021-04-27 |
Family
ID=61043385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711016779.6A Active CN107586728B (en) | 2017-10-26 | 2017-10-26 | Cordyceps sinensis culture solution and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107586728B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439495A (en) * | 2018-11-20 | 2019-03-08 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of dandelion fermented wine and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220249A (en) * | 2011-06-13 | 2011-10-19 | 杭州柯氏生物科技有限公司 | Method for producing Hirsutella sinensis |
| CN103444434A (en) * | 2013-08-27 | 2013-12-18 | 王辉 | Fermentation raw material treatment method for improving yield and quality of cordyceps sinensis |
| CN105624200A (en) * | 2016-02-05 | 2016-06-01 | 浙江工业大学 | Recycling method of hirsutella sinensis fermentation filtrate |
| CN106190866A (en) * | 2016-08-11 | 2016-12-07 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of reuse method of Cordyceps fermented waste fluid |
-
2017
- 2017-10-26 CN CN201711016779.6A patent/CN107586728B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220249A (en) * | 2011-06-13 | 2011-10-19 | 杭州柯氏生物科技有限公司 | Method for producing Hirsutella sinensis |
| CN103444434A (en) * | 2013-08-27 | 2013-12-18 | 王辉 | Fermentation raw material treatment method for improving yield and quality of cordyceps sinensis |
| CN105624200A (en) * | 2016-02-05 | 2016-06-01 | 浙江工业大学 | Recycling method of hirsutella sinensis fermentation filtrate |
| CN106190866A (en) * | 2016-08-11 | 2016-12-07 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of reuse method of Cordyceps fermented waste fluid |
Non-Patent Citations (2)
| Title |
|---|
| J. GANG等: "Optimization of Liquid Fermentation Conditions and Protein Nutrition Evaluation of Mycelium from the Caterpillar Medicinal Mushroom, Cordyceps militaris (Ascomycetes)", 《INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS》 * |
| 张楠等: "蛹虫草深层发酵产虫草素培养基的优化", 《北方园艺》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109439495A (en) * | 2018-11-20 | 2019-03-08 | 青海珠峰冬虫夏草工程技术研究有限公司 | A kind of dandelion fermented wine and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107586728B (en) | 2021-04-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104187746B (en) | The technique that fermentation method prepares Testa Phaseoli radiati water soluble dietary fiber | |
| CN103396973B (en) | Method for producing mycoprotein feed through microbial fermentation of kitchen wastes | |
| CN108118001A (en) | A kind of cordyceps sinensis fluid nutrient medium and preparation method thereof | |
| CN101665373A (en) | Safe and high-efficiency preparation method of master culture medium material of edible fungi | |
| CN104996729B (en) | Comprehensive recycling technology of radix ophiopogonis decoction dregs | |
| CN104718983B (en) | A kind of strain domestication method of artificial cultivation umbellate pore furgus | |
| CN105211555A (en) | One utilizes edible fungi residue fermenting and producing cow protein feed new method | |
| CN101701194B (en) | Method for producing cellulose and polypeptide protein feed by fermenting with Aspergillus awamori | |
| CN110684672B (en) | Fermentation method of antioxidant cordyceps sobolifera mycelium | |
| CN109429911A (en) | A kind of fermentation cordyceps sinensis culture solution and preparation method thereof prepared with jerusalem artichoke | |
| CN107586728A (en) | A kind of cordyceps sinensis nutrient solution and preparation method thereof | |
| CN106719948A (en) | A kind of preparation method of biological pesticide | |
| CN108165421A (en) | A kind of preparation method with the sealwort adlay health liquor for enhancing immune function | |
| CN104286383A (en) | Tea seed meal detoxifying method | |
| CN107177515A (en) | A kind of ganoderma lucidum solid spawn and its application in ganoderma lucidum liquid submerged fermentation | |
| CN104496688B (en) | The application of ganoderma lucidum solid state cultivation culture medium and MAILUONING ZHUSHEYE production discarded object in cultivation of glossy ganoderma | |
| CN101579039B (en) | Method for producing animal feed from oil-tea-cake dregs fermented by neurospora crassa | |
| CN103393128B (en) | Method for preparing dietary fibers by agaricus brasiliensis liquid-based fermentation of grifola frondosus residues | |
| CN102876588A (en) | High color value highland barley monascus and preparation method thereof | |
| CN102584363B (en) | Method for producing medical mycelium or medical and edible dual-purpose mycelium by using yellow wine lees as liquid medium | |
| CN206052011U (en) | Black tea bacterium solution fermentation machine structure | |
| CN109022501A (en) | A method of ferulic acid is obtained using waste | |
| CN107090485A (en) | A kind of method and its special culture media and complex bacterial agent special for preparing active polysaccharide | |
| CN106801074A (en) | A kind of gliocladin bacterium dregs innocent treatment method | |
| CN101134940B (en) | Fermentation production method for Chinese caterpillar fungus fungus-bat moth hirsutella sinensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |