CN107586170B - Leaf vegetable nutrient and application thereof - Google Patents

Leaf vegetable nutrient and application thereof Download PDF

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CN107586170B
CN107586170B CN201711007104.5A CN201711007104A CN107586170B CN 107586170 B CN107586170 B CN 107586170B CN 201711007104 A CN201711007104 A CN 201711007104A CN 107586170 B CN107586170 B CN 107586170B
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CN107586170A (en
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高洪波
吴晓蕾
李敬蕊
宫彬彬
吕桂云
高军
章铁军
高秀瑞
田真
王祥
王宁
贾邱颖
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Heibei Agricultural University
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Abstract

The invention provides a leaf vegetable nutrient which comprises the following components: gamma-aminobutyric acid, phosphorus, potassium, calcium, magnesium, sulfur, iron, boron, manganese, zinc, copper, molybdenum, polysorbate-80, silicone, and organically chelated titanium; setting leaf surface spraying, root system spraying or seed soaking spraying wet application methods according to different production requirements, and setting different concentration ratios and application conditions aiming at different application methods; the leaf vegetable nutritional agent and the application method thereof can effectively improve the quality of leaf vegetables, increase the yield of the leaf vegetables, increase the amino acid content, especially the GABA content, of the leaf vegetables, and reduce the nitrate content of the leaf vegetables; the fertilizer has the advantages of sufficient nutrients, scientific and reasonable proportioning, simple preparation and use and low price, and can be suitable for different leaf vegetable cultivation modes such as soil cultivation, soilless cultivation and the like.

Description

Leaf vegetable nutrient and application thereof
Technical Field
The invention relates to the technical field of vegetable cultivation, in particular to a leaf vegetable nutrient and an application method thereof.
Background
The leaf vegetables are vegetables taking tender leaves and petioles of plants as edible parts and account for 1/3-2/3 of vegetable biomass. Common leafy vegetables include rape, leek, lettuce, celery, spinach, amaranth, endive, cabbage, garland chrysanthemum, agaric and the like, and can be divided into three types of common leafy vegetables, noduliflower vegetables and spices according to cultivation characteristics. Because leaf vegetables grow fast, the cultivation period is short, and the fertilizer demand is large, in the traditional planting mode, a method of applying organic fertilizer and inorganic fertilizer mainly comprising nitrogen, phosphorus and potassium in a matching way is often adopted to meet the production requirement, so that the nutrient utilization rate is low, the damage of the soil structure is easily caused, the accumulation of harmful substances and plant diseases and insect pests in the soil are increased, and the yield and the quality of the leaf vegetables are further influenced.
Most seriously, in the current vegetable planting production process, the phenomenon that a producer applies excessive or excessive chemical fertilizers and organic fertilizers to improve the yield is gradually serious, and the caused consequences mainly comprise the following aspects: (1) although the organic fertilizer is used in a large amount, the soil hardening phenomenon can be relieved to a certain extent, but antibiotics, hormones and the like in the organic fertilizer become main sources of soil pollution; (2) the undegraded organic fertilizer becomes a main source of soil diseases and insect pests; (3) the nutrition proportion of the organic fertilizer does not meet the growth requirement of vegetables, and the residual nutrition can also be the main reason of secondary salinization of soil. Meanwhile, vegetable nutrient deficiency and nitrate content exceeding are easily caused by unbalanced proportion of organic fertilizers and inorganic fertilizers and excessive application of fertilizers, so that research on a leaf vegetable nutrient with balanced nutrition and capability of effectively promoting vegetable quality and yield and an application method thereof are urgently needed.
Disclosure of Invention
Based on the defects of the prior art, the invention provides the leaf vegetable nutrient which can effectively promote the growth of leaf vegetables and improve the quality and the yield of the vegetables and the application method thereof.
The technical scheme adopted by the invention to realize the purpose is as follows:
the leaf vegetable nutrient is characterized by comprising the following components: gamma-aminobutyric acid, phosphorus, potassium, calcium, magnesium, sulfur, iron, boron, manganese, zinc, copper, molybdenum, polysorbate-80, silicone, and organically chelated titanium;
wherein, when being used for foliage spray, include: 50-80mmol/L of gamma-aminobutyric acid, 0.5-1.0mmol/L of phosphorus, 4-6mmol/L of potassium, 2-3mmol/L of calcium, 1-2mmol/L of magnesium, 1-2mmol/L of sulfur, 50-100 mu mol/L of iron, 50-60 mu mol/L of boron, 5-10 mu mol/L of manganese, 0.7-1.0 mu mol/L of zinc, 0.1-0.2 mu mol/L of copper, 0.05-0.1 mu mol/L of molybdenum, 805-6g/L of polysorbate-organic silicon, 0.05-0.3g/L of organic silicon and 5-20mg/L of organic chelated titanium;
or when the fertilizer is used for root system dressing or seed soaking treatment, the gamma-aminobutyric acid accounts for 15-30mmol/L, the phosphorus accounts for 1.0-1.5mmol/L, the potassium accounts for 6-8mmol/L, the calcium accounts for 3-5mmol/L, the magnesium accounts for 2-3mmol/L, the sulfur accounts for 1-2mmol/L, the iron accounts for 50-100 mu mol/L, the boron accounts for 60-80 mu mol/L, the manganese accounts for 10-15 mu mol/L, the zinc accounts for 1.0-1.5 mu mol/L, the copper accounts for 0.2-0.3 mu mol/L, the molybdenum accounts for 0.1-0.2 mu mol/L, the polysorbate accounts for-805-6 g/L, the organic silicon accounts for 0.05-0.3g/L, and the organic chelated titanium accounts for 5-20 mg/L.
Further, when the leaf vegetable nutrient is used for foliage spraying, the leaf vegetable nutrient comprises the following components: 60-70mmol/L of gamma-aminobutyric acid, 0.6-0.8mmol/L of phosphorus, 4.5-5.5mmol/L of potassium, 2.2-2.8mmol/L of calcium, 1.2-1.8mmol/L of magnesium, 1.2-1.8mmol/L of sulfur, 60-80 mu mol/L of iron, 52-58 mu mol/L of boron, 6-8 mu mol/L of manganese, 0.8-0.9 mu mol/L of zinc, 0.12-0.18 mu mol/L of copper, 0.06-0.1 mu mol/L of molybdenum, 805.2-5.8g/L of polysorbate-890.25 g/L of organic silicon and 5-15mg/L of organic chelated titanium;
or when the fertilizer is used for root system dressing or seed soaking treatment, the fertilizer comprises the following components: 20-25mmol/L of gamma-aminobutyric acid, 1.2-1.4mmol/L of phosphorus, 6.5-7.5mmol/L of potassium, 3.5-4.5mmol/L of calcium, 2.2-2.8mmol/L of magnesium, 1.2-1.8mmol/L of sulfur, 60-80 mu mol/L of iron, 65-75 mu mol/L of boron, 12-15 mu mol/L of manganese, 1.2-1.4 mu mol/L of zinc, 0.22-0.28 mu mol/L of copper, 0.1-0.2 mu mol/L of molybdenum, 805.2-5.8g/L of polysorbate-890.25 g/L of organic silicon and 5-15mg/L of organic chelated titanium.
A method for applying a leaf vegetable nutrient is characterized in that: when the foliar fertilizer is used for foliar spraying, foliar spraying is carried out 6-8 days before harvesting, and the dosage per mu is 30L.
Further, the leaf surface spraying is carried out under the conditions that the temperature is more than 15 ℃ and the illumination intensity is 5000-.
A method for applying a leaf vegetable nutrient is used for irrigating plants 6-8 days before harvesting when root systems are applied, and the irrigation amount of each plant is 50-100 mL.
Further, the root system dressing is carried out under the conditions that the temperature of the root system is more than 15 ℃, the soil humidity is about 70 percent and the illumination is 5000-.
A method for applying a leaf vegetable nutrient is characterized in that: when the method is used for seed soaking treatment, neat and full leaf vegetable seeds are selected, soaked in warm water at 55 ℃ for 15-20min 1 day before sowing, soaked in a nutrient for 6-12h after the water temperature is restored to room temperature, and then normally managed and planted.
Has the advantages that: the leaf vegetable nutrient provided by the invention has the advantages of sufficient nutrients, scientific and reasonable proportioning, simple preparation and use and low price, and can be suitable for different leaf vegetable cultivation modes such as soil cultivation, soilless cultivation and the like; the method comprises the steps of setting leaf surface spraying, root system spraying or seed soaking spraying wet application methods according to different production requirements, setting different concentration ratios and application conditions according to different application methods, further effectively improving the quality of leaf vegetables, increasing the yield of the leaf vegetables, increasing the amino acid content of the leaf vegetables, particularly the GABA content, reducing the nitrate content of the leaf vegetables and reducing the continuous cropping obstacle influence.
Detailed Description
The present invention will be described in further detail with reference to examples.
Among the nutrient elements, phosphorus is provided by monoammonium phosphate, potassium is provided by potassium sulfate, calcium is provided by EDTA chelated calcium, magnesium is provided by magnesium chloride, sulfur is provided by potassium sulfate, ferrous sulfate and the like, iron is provided by ferrous sulfate, boron is provided by boric acid, manganese is provided by manganese sulfate, zinc is provided by zinc sulfate, copper is provided by copper sulfate, and molybdenum is provided by sodium molybdate.
Example 1
(1) Preparation of nutrient
Preparing a leaf vegetable nutrient 1: 50mmol/L gamma-aminobutyric acid, 0.5mmol/L phosphorus, 4mmol/L potassium, 2mmol/L calcium, 1mmol/L magnesium, 1mmol/L sulfur, 50. mu. mol/L iron, 50. mu. mol/L boron, 5. mu. mol/L manganese, 0.7. mu. mol/L zinc, 0.1. mu. mol/L copper, 0.1. mu. mol/L molybdenum, polysorbate-805 g/L silicone, 0.05g/L organic chelate titanium, 5mg/L organic chelate titanium, and the balance of water;
preparing a leaf vegetable nutrient 2: 60mmol/L gamma-aminobutyric acid, 0.6mmol/L phosphorus, 4.5mmol/L potassium, 2.2mmol/L calcium, 1.2mmol/L magnesium, 1.2mmol/L sulfur, 60 mu mol/L iron, 52 mu mol/L boron, 6 mu mol/L manganese, 0.8 mu mol/L zinc, 0.12 mu mol/L copper, 0.1 mu mol/L molybdenum, 805.2g/L polysorbate-805.2 g/L organosilicon, 10mg/L titanium chelate, and the balance water;
preparing a leaf vegetable nutrient 3: 65mmol/L gamma-aminobutyric acid, 0.7mmol/L phosphorus, 5mmol/L potassium, 2.5mmol/L calcium, 1.5mmol/L magnesium, 1.5mmol/L sulfur, 70. mu. mol/L iron, 55. mu. mol/L boron, 7. mu. mol/L manganese, 0.85. mu. mol/L zinc, 0.15. mu. mol/L copper, 0.1. mu. mol/L molybdenum, polysorbate-805.5 g/L organosilicon, 15mg/L organic chelated titanium, and the balance of water;
preparing a leaf vegetable nutrient 4: gamma-aminobutyric acid 70mmol/L, phosphorus 0.8mmol/L, potassium 5.5mmol/L, calcium 2.8mmol/L, magnesium 1.8mmol/L, sulfur 1.8mmol/L, iron 80. mu. mol/L, boron 58. mu. mol/L, manganese 8. mu. mol/L, zinc 0.9. mu. mol/L, copper 0.18. mu. mol/L, molybdenum 0.1. mu. mol/L, polysorbate-805.8 g/L, organosilicon 0.25g/L, titanium chelate 15mg/L, and the balance of water;
preparing a leaf vegetable nutrient 5: 80mmol/L gamma-aminobutyric acid, 1.0mmol/L phosphorus, 6mmol/L potassium, 3mmol/L calcium, 2mmol/L magnesium, 2mmol/L sulfur, 100. mu. mol/L iron, 60. mu. mol/L boron, 10. mu. mol/L manganese, 1.0. mu. mol/L zinc, 0.2. mu. mol/L copper, 0.1. mu. mol/L molybdenum, 806g/L polysorbate, 0.3g/L organosilicon, 20mg/L titanium chelate, and the balance of water.
(2) Design of experiments
The experiment is a field plot experiment, and the area of each plot is 667m2The method comprises the steps of selecting No. 3 Beijing spinach as a material, directly sowing vegetable seeds with the planting density of 15 × 15cm, managing according to a conventional method after seedling emergence, sowing for 35 days, carrying out leaf surface spraying treatment on each cell by using No. 1-5 leaf vegetable nutritional agents respectively as an experimental group 1-5 with the dosage of 30L per mu, spraying and selecting under the conditions that the temperature is higher than 15 ℃ and the illumination is 5000-.
Further, a control group not subjected to the foliar spray treatment was set as a control group 1;
the Hoagland general formula is used as a nutrient to replace an experimental group nutrient for carrying out foliage spraying treatment, the dosage per mu is 30L, and the Hoagland general formula is used as a control group 2; the concentration of each component is 16mmol/L of total nitrogen, 2.0mmol/L of phosphorus, 6.0mmol/L of potassium, 4.0mmol/L of calcium, 1.0mmol/L of magnesium, 1.0mmol/L of sulfur, 53.0 mu mol/L of iron, 25.0 mu mol/L of boron, 2.0 mu mol/L of manganese, 2.0 mu mol/L of zinc, 0.5 mu mol/L of copper and 0.5 mu mol/L of molybdenum.
After 30 days of sowing, carrying out foliage spraying treatment by using the leaf vegetable nutrient 1, continuing to culture for 12 days after the spraying treatment, and then measuring related indexes to serve as a control group 3;
after sowing for 40 days, carrying out foliage spraying treatment by using the leaf vegetable nutrient 1, continuing to culture for 2 days after the spraying treatment, and then measuring related indexes to serve as a control group 4;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the dosage per mu is 40L, and the control group is 5;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the dosage of each mu is 20L, and the leaf vegetable nutritional agent is used as a control group 6;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the spraying temperature is 12 ℃, and the spraying temperature is used as a control group 7;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the gamma-aminobutyric acid is replaced by proline to be used as a control group 8;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the gamma-aminobutyric acid is replaced by 20mmol/L and is used as a control group 9;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the gamma-aminobutyric acid is replaced by 100mmol/L and is used as a control group 10.
The control group had the same parameters as the experimental group 1 except for the specified parameters.
(3) And (4) analyzing results:
the nitrate content, the gamma-aminobutyric acid content, the leaf number, the maximum leaf length, the maximum leaf width, the maximum root length and the yield index of spinach are measured during harvest, and the average value of the measurement results of three cells is taken, and the results are shown in table 1:
TABLE 1
Figure BDA0001444642640000061
Figure BDA0001444642640000071
Example 2
(1) Preparation of nutrient
Preparing a leaf vegetable nutrient 6: 15mmol/L gamma-aminobutyric acid, 1.0mmol/L phosphorus, 6mmol/L potassium, 3mmol/L calcium, 2mmol/L magnesium, 1mmol/L sulfur, 50. mu. mol/L iron, 60. mu. mol/L boron, 15. mu. mol/L manganese, 1.0. mu. mol/L zinc, 0.2. mu. mol/L copper, 0.2. mu. mol/L molybdenum, polysorbate-805 g/L silicone, 0.05g/L organic chelate titanium, 5mg/L organic chelate titanium, and the balance of water;
preparing a leaf vegetable nutrient 7: 20mmol/L gamma-aminobutyric acid, 1.2mmol/L phosphorus, 6.5mmol/L potassium, 3.5mmol/L calcium, 2.2mmol/L magnesium, 1.2mmol/L sulfur, 60 mu mol/L iron, 65 mu mol/L boron, 15 mu mol/L manganese, 1.2 mu mol/L zinc, 0.22 mu mol/L copper, 0.2 mu mol/L molybdenum, 805.2g/L polysorbate-805.2 g/L organosilicon, 10mg/L titanium chelate, and the balance water;
preparing a leaf vegetable nutrient 8: 25mmol/L gamma-aminobutyric acid, 1.3mmol/L phosphorus, 7mmol/L potassium, 4mmol/L calcium, 2.5mmol/L magnesium, 1.5mmol/L sulfur, 70. mu. mol/L iron, 70. mu. mol/L boron, 15. mu. mol/L manganese, 1.3. mu. mol/L zinc, 0.25. mu. mol/L copper, 0.2. mu. mol/L molybdenum, polysorbate-805.5 g/L, 0.2g/L organosilicon, 15mg/L organic chelate titanium, and the balance of water;
preparing a leaf vegetable nutrient 9: 25mmol/L gamma-aminobutyric acid, 1.4mmol/L phosphorus, 7.5mmol/L potassium, 4.5mmol/L calcium, 2.8mmol/L magnesium, 1.8mmol/L sulfur, 80. mu. mol/L iron, 75. mu. mol/L boron, 15. mu. mol/L manganese, 1.4. mu. mol/L zinc, 0.28. mu. mol/L copper, 0.2. mu. mol/L molybdenum, 805.8g/L polysorbate-805.8 g/L, 0.25g/L organosilicon, 15mg/L titanium chelate, and the balance water;
preparing a leaf vegetable nutrient 10: 30mmol/L gamma-aminobutyric acid, 1.5mmol/L phosphorus, 8mmol/L potassium, 5mmol/L calcium, 3mmol/L magnesium, 2mmol/L sulfur, 100. mu. mol/L iron, 80. mu. mol/L boron, 15. mu. mol/L manganese, 1.5. mu. mol/L zinc, 0.3. mu. mol/L copper, 0.2. mu. mol/L molybdenum, 806g/L polysorbate, 0.3g/L organosilicon, 20mg/L titanium chelate, and the balance of water.
(2) Design of experiments
The experiment is a field plot experiment, and the area of each plot is 667m2The method comprises the steps of selecting 'Jingbo No. 3', directly sowing vegetable seeds with the planting density of 15 × 15cm, managing according to a conventional method after seedling emergence, sowing for 35 days, respectively carrying out root system dressing treatment on each cell by using leaf vegetable nutrient No. 6-10, wherein the watering amount of each plant is 80mL, the plant is used as an experimental group 6-10, carrying out root system dressing selection under the conditions that the temperature is higher than 15 ℃ and the illumination is 5000 plus materials 30000Lux, setting three parallels for each treatment, continuously culturing for 7 days after dressing treatment, and then randomly selecting 20 plants in each cell to measure relevant indexes.
Further, a control group not subjected to root dressing treatment was set as a control group 11;
root system dressing treatment is carried out by taking a Hoagland general formula as a nutrient instead of a nutrient of an experimental group, the irrigation amount of each plant is 80mL, and the Hoagland general formula is used as a control group 12; the concentration of each component is 16mmol/L of total nitrogen, 2.0mmol/L of phosphorus, 6.0mmol/L of potassium, 4.0mmol/L of calcium, 1.0mmol/L of magnesium, 1.0mmol/L of sulfur, 53.0 mu mol/L of iron, 25.0 mu mol/L of boron, 2.0 mu mol/L of manganese, 2.0 mu mol/L of zinc, 0.5 mu mol/L of copper and 0.5 mu mol/L of molybdenum.
After 30 days of sowing, root system dressing treatment is carried out on the leaf vegetable nutrient 6, the cultivation is continued for 12 days after the dressing treatment, and then the related indexes are measured to be used as a control group 13;
after sowing for 40 days, carrying out root system dressing treatment by using the leaf vegetable nutrient 6, continuing to culture for 2 days after dressing treatment, and then measuring related indexes to serve as a control group 14;
root system dressing treatment is carried out on the leaf vegetable nutrient 6, the irrigation amount of each plant is 150mL, and the plant is used as a control group 15;
root system dressing treatment is carried out on the leaf vegetable nutrient 6, the irrigation amount of each plant is 20mL, and the plant is used as a control group 16;
carrying out root dressing treatment by using a leaf vegetable nutrient 6 at the temperature of 12 ℃ as a control group 17;
carrying out root system dressing treatment by using a leaf vegetable nutrient 6, wherein the gamma-aminobutyric acid is replaced by proline to be used as a control group 18;
carrying out root system dressing treatment by using a leaf vegetable nutrient 6, wherein the gamma-aminobutyric acid is replaced by 10mmol/L and is used as a control group 19;
root system dressing treatment is carried out by using the leaf vegetable nutrient 6, wherein gamma-aminobutyric acid is replaced by 40mmol/L and is used as a control group 20.
The control group had the same parameters as the experimental group 6 except for the specified parameters.
(3) And (4) analyzing results:
the nitrate content, the gamma-aminobutyric acid content, the leaf number, the maximum leaf length, the maximum leaf width, the maximum root length and the yield index of spinach are measured during harvest, and the average value of the measurement results of three cells is taken, and the results are shown in table 2:
TABLE 2
Figure BDA0001444642640000091
Figure BDA0001444642640000101
Example 3
(1) Preparation of nutrient
6-10 leaf vegetable nutrient as in example 2 is prepared.
(2) Design of experiments
The experiment is a field plot experiment, and the area of each plot is 667m2The method comprises the steps of selecting neat and plump seeds of 'Jingbo No. 3', soaking the seeds 1 day before sowing for 20min in warm water at 55 ℃, soaking the seeds for 8h in a leaf vegetable nutrient agent 6-10 after the water temperature is restored to the room temperature to serve as an experimental group 11-15, soaking the seeds in the leaf vegetable nutrient agent 6 to serve as an experimental group 16, soaking the seeds in the leaf vegetable nutrient agent 6 to serve as an experimental group 17, wherein the planting density is 15 × 15cm, normal cultivation management can be carried out, setting three parallel processes for each process, and randomly selecting 20 plants in each plot after sowing for 40 days to measure relevant indexes.
Further, a control group not subjected to seed soaking treatment was set as a control group 21;
adding polysorbate-80, organic silicon and organic chelated titanium as a nutrient to replace a nutrient of an experimental group for seed soaking treatment by using a Hoagland general formula to serve as a control group 22; the concentration of each component is 16mmol/L of total nitrogen, 2.0mmol/L of phosphorus, 6.0mmol/L of potassium, 4.0mmol/L of calcium, 1.0mmol/L of magnesium, 1.0mmol/L of sulfur, 53.0 mu mol/L of iron, 25.0 mu mol/L of boron, 2.0 mu mol/L of manganese, 2.0 mu mol/L of zinc, 0.5 mu mol/L of copper, 0.5 mu mol/L of molybdenum, polysorbate-805 g/L, 0.05g/L of organic silicon, 5mg/L of organic chelate titanium, and the balance of water.
Soaking the seeds in the leaf vegetable nutritional agent 6 without soaking in warm water before soaking the seeds to obtain a control group 23;
soaking the seeds in the leaf vegetable nutritional agent 6 at 35 deg.C before soaking as a control group 24;
soaking the seeds in the leaf vegetable nutritional agent 6 for 3h to serve as a control group 25;
soaking the seeds in the leaf vegetable nutritional agent 6 for 18h to serve as a control group 26;
soaking the seeds by using a leaf vegetable nutritional agent 6, wherein the gamma-aminobutyric acid is replaced by proline to be used as a control group 27;
soaking the seeds by using a leaf vegetable nutritional agent 6, wherein the gamma-aminobutyric acid is replaced by 10mmol/L to serve as a control group 28;
soaking the seeds with leaf vegetable nutritional agent 6, wherein gamma-aminobutyric acid is replaced by 40mmol/L to serve as a control group 29.
The control group had the same parameters as the experimental group 11 except for the specified parameters.
(3) And (4) analyzing results:
the nitrate content, the gamma-aminobutyric acid content, the leaf number, the maximum leaf length, the maximum leaf width, the maximum root length and the yield index of spinach are measured during harvest, and the average value of the measurement results of three cells is taken, and the results are shown in table 3:
TABLE 3
Figure BDA0001444642640000111
Figure BDA0001444642640000121
Example 4
(1) Preparation of nutrient
Leaf vegetable nutritional agents 1 to 5 in example 1 were prepared.
(2) Design of experiments
The experiment is a water culture vegetable experiment, the 'big leaf agaric vegetable' is selected as a material, seeds are sown in a seedling tray, when 3-4 leaves grow out from seedlings, the seedlings are randomly selected and transplanted into a water culture experimental groove (the length is multiplied by the width is multiplied by the depth is 4.5m multiplied by 0.7m multiplied by 0.2m), and the cultivation density is 20cm multiplied by 20 cm.
After 20 days of culture, vegetables in leaf vegetable nutrient agent 1-5 water culture experimental grooves are respectively adopted to carry out one-time leaf surface spraying treatment to serve as experimental groups 18-22, the dosage per mu of land is 30L, and the application amount of the nutrient agent in each water culture experimental groove is 280 mL. The spraying selection is carried out under the conditions that the temperature is more than 15 ℃ and the illumination is 5000-. Three treatments are set in parallel, the culture is continued for 7 days after the spraying treatment, and then 20 plants are randomly selected from each water culture experimental tank to determine relevant indexes.
Further, a control group not subjected to the foliar spray treatment was set as a control group 30;
organic silicon and organic chelated titanium are added into the Hoagland general formula to serve as a nutrient to replace a nutrient of an experimental group for carrying out foliage spraying treatment, and the Hoagland general formula serves as a control group 31; the concentration of each component is 16mmol/L of total nitrogen, 2.0mmol/L of phosphorus, 6.0mmol/L of potassium, 4.0mmol/L of calcium, 1.0mmol/L of magnesium, 1.0mmol/L of sulfur, 53.0 mu mol/L of iron, 25.0 mu mol/L of boron, 2.0 mu mol/L of manganese, 2.0 mu mol/L of zinc, 0.5 mu mol/L of copper, 0.5 mu mol/L of molybdenum and Mo, 0.1g/L of organic silicon, 20mg/L of organic chelated titanium, and the balance of water.
After 15 days of water culture, carrying out foliage spraying treatment by using the leaf vegetable nutrient 1, continuing to culture for 12 days after spraying treatment, and then determining related indexes to serve as a control group 32;
carrying out leaf surface spraying treatment by using a leaf vegetable nutrient 1 after 25 days of water culture, continuing to culture for 2 days after spraying treatment, and then measuring related indexes to serve as a control group 33;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the dosage per mu is 40L, and the control group is 34;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the dosage of each mu is 20L, and the leaf vegetable nutritional agent is used as a control group 35;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the spraying temperature is 12 ℃, and the control group is 36;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the gamma-aminobutyric acid is replaced by proline to be used as a control group 37;
carrying out foliage spraying treatment by using the leaf vegetable nutritional agent 1, wherein the gamma-aminobutyric acid is replaced by 20mmol/L and is used as a control group 38;
the leaf vegetable nutritional agent 1 is used for carrying out leaf surface spraying treatment, wherein the gamma-aminobutyric acid is replaced by 100mmol/L to be used as a control group 39.
The control group had the same parameters as the experimental group 18 except for the specified parameters.
(3) And (4) analyzing results:
the nitrate content, the gamma-aminobutyric acid content, the leaf number, the maximum leaf length, the maximum leaf width, the maximum root length and the yield index of the agaric vegetable are measured during harvest of the agaric vegetable, the average value of the measurement results of three water culture experimental tanks is taken, and the results are shown in table 4:
TABLE 4
Figure BDA0001444642640000141
Example 5
(1) Preparation of nutrient
The leaf vegetable nutrient 1 of example 1 was prepared.
(2) Design of experiments
The experiment is a field plot experiment, and the area of each plot is 667m2Selecting American fast-growing lettuce as material, and directly sowingScattering vegetable seeds with the planting density of 15cm × 20cm, managing according to a conventional method after seedling emergence, sowing for 40 days, carrying out leaf surface spraying treatment on each cell by using a leaf vegetable nutrient agent No. 1 as an experimental group 23, wherein the dosage of each mu is 30L, spraying is carried out under the conditions that the temperature is higher than 15 ℃ and the illumination is 5000-.
Further, control groups not subjected to foliar spray treatment were set, and three times of planting as described above were carried out, respectively, as control groups 40 to 42.
(3) And (4) analyzing results:
the nitrate content, the gamma-aminobutyric acid content, the leaf number, the maximum leaf length, the maximum leaf width, the maximum root length and the yield index of the lettuce are measured when the lettuce is harvested, the average value of the measurement results is taken, and the results are shown in table 1:
TABLE 5
Figure BDA0001444642640000151
Example 6
(1) Preparation of nutrient
Leaf vegetable nutrient 1 in example 1 and leaf vegetable nutrient 6 in example 2 were prepared.
(2) Design of experiments
The experiment is a field plot experiment, and the area of each plot is 667m2Selecting regular and plump seeds of large-leaf crowndaisy chrysanthemum, soaking the seeds in warm water at 55 ℃ for 15min 1 day before sowing, soaking the seeds in leaf vegetable nutritional agent 6 for 8h after the water temperature is restored to the room temperature, then carrying out normal management and field planting, wherein the planting density is 30cm, × 35cm, managing according to a conventional method after seedling emergence, carrying out leaf surface spraying treatment on each plot once by using the leaf vegetable nutritional agent 1 after sowing for 35 days, wherein the dosage per mu is 30L, and sprayingThe selection is carried out under the conditions that the temperature is more than 15 ℃ and the illumination is 5000-. Three parallel lines are set, the cultivation is continued for 8 days after the spraying treatment, and then 20 plants are randomly selected from each cell for measuring related indexes. The above experiment was designated as experimental group 26.
Further, a control group not subjected to seed soaking and foliar spray treatment was set as the control group 43.
Further, a control group in which seed soaking was performed but no foliar spray treatment was performed was set as the control group 44.
Further, a control group in which the seed soaking was not performed but the foliar spray treatment was performed was set as the control group 45.
The control groups 43-45 were identical to the experimental group 26 except for the parameters specified.
(3) And (4) analyzing results:
measuring the nitrate content, the gamma-aminobutyric acid content, the leaf number, the maximum leaf length, the maximum leaf width, the maximum root length and the yield index of the crowndaisy chrysanthemum when the crowndaisy chrysanthemum is harvested, and taking the average value of the measurement results, wherein the results are shown in a table 1:
TABLE 6
Figure BDA0001444642640000161
Example 7
(1) Preparation of nutrient
The leaf vegetable nutrient 6 of example 2 was prepared.
(2) Design of experiments
The experiment is a field plot experiment, and the area of each plot is 667m2Selecting neat and plump seeds of red amaranth, soaking the seeds in warm water at 55 ℃ for 15min 1 day before sowing, soaking the seeds in leaf vegetable nutrient 6 for 8h after the water temperature is restored to the room temperature, then normally managing and planting, wherein the planting density is 30cm, × 33cm and 33cm, managing according to a conventional method after seedling emergence, performing root system dressing treatment on each plot once by using the leaf vegetable nutrient 6 after sowing for 35 days, wherein the watering amount of each plant is 80mL, the root system dressing is performed under the conditions that the temperature is higher than 15 ℃ and the illumination is 5000-year 30000Lux, and each site is selectedThree parallel lines are arranged, the cultivation is continued for 8 days after the additional treatment, and then 20 plants are randomly selected from each cell for measuring relevant indexes. The above experiment was taken as experiment group 27.
Further, a control group not subjected to seed soaking and root dressing was set as a control group 46.
Further, a control group in which the seed was soaked but the root system dressing treatment was not performed was provided as the control group 47.
Further, a control group in which the seed soaking was not performed but the root system dressing treatment was performed was set as the control group 48.
The control groups 46-48 were identical to the experimental group 27 except for the parameters specified.
(3) And (4) analyzing results:
the nitrate content, the gamma-aminobutyric acid content, the leaf number, the maximum leaf length, the maximum leaf width, the maximum root length and the yield index of the amaranth are measured during harvesting of the amaranth, and the average value of the measurement results is taken, and the results are shown in table 1:
TABLE 7
Figure BDA0001444642640000171
Figure BDA0001444642640000181
The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the examples shown herein, and all technical solutions belonging to the idea of the present invention belong to the protection scope of the present invention. It should be noted that equivalent substitutions of raw materials and addition or subtraction of auxiliary components for those skilled in the art can be made without departing from the principle of the present invention, and the scope of the present invention is also within the protection and disclosure of the present invention.

Claims (2)

1. A method for applying a leaf vegetable nutrient is characterized in that,
selecting neat and full leaf vegetable seeds, soaking the seeds in warm water at 55 ℃ for 15-20min 1 day before sowing, soaking the seeds in a leaf vegetable nutrient for 6-12h after the water temperature is restored to room temperature, then carrying out normal management and field planting, and carrying out leaf surface spraying or root system dressing by using the leaf vegetable nutrient 6-8 days before harvesting;
when the foliar fertilizer is used for foliar spraying, the dosage of the foliar vegetable nutrient is 30L per mu; the leaf surface spraying is carried out under the conditions that the temperature is more than 15 ℃ and the illumination intensity is 5000-;
when the fertilizer is used for root system dressing, the watering amount of each plant of the leaf vegetable nutrient is 50-100 mL; the root system dressing is carried out under the conditions that the temperature of the root system is more than 15 ℃, the soil humidity is 70 percent and the illumination is 5000-;
the leaf vegetable nutrient comprises the following components: gamma-aminobutyric acid, phosphorus, potassium, calcium, magnesium, sulfur, iron, boron, manganese, zinc, copper, molybdenum, polysorbate-80, silicone, and organically chelated titanium;
wherein, when being used for foliage spray, include: 50-80mmol/L of gamma-aminobutyric acid, 0.5-1.0mmol/L of phosphorus, 4-6mmol/L of potassium, 2-3mmol/L of calcium, 1-2mmol/L of magnesium, 1-2mmol/L of sulfur, 50-100 mu mol/L of iron, 50-60 mu mol/L of boron, 5-10 mu mol/L of manganese, 0.7-1.0 mu mol/L of zinc, 0.1-0.2 mu mol/L of copper, 0.05-0.1 mu mol/L of molybdenum, 805-6g/L of polysorbate-organic silicon, 0.05-0.3g/L of organic silicon and 5-20mg/L of organic chelated titanium;
when the fertilizer is used for root system dressing or seed soaking treatment, 15-30mmol/L of gamma-aminobutyric acid, 1.0-1.5mmol/L of phosphorus, 6-8mmol/L of potassium, 3-5mmol/L of calcium, 2-3mmol/L of magnesium, 1-2mmol/L of sulfur, 50-100 mu mol/L of iron, 60-80 mu mol/L of boron, 10-15 mu mol/L of manganese, 1.0-1.5 mu mol/L of zinc, 0.2-0.3 mu mol/L of copper, 0.1-0.2 mu mol/L of molybdenum, 805-6g/L of polysorbate-0.3 g/L of organic silicon and 5-20mg/L of organic chelated titanium.
2. The method for applying the leaf vegetable nutrient according to claim 1, wherein the method comprises the following steps:
when the leaf vegetable nutritional agent is used for foliage spraying, the method comprises the following steps: 60-70mmol/L of gamma-aminobutyric acid, 0.6-0.8mmol/L of phosphorus, 4.5-5.5mmol/L of potassium, 2.2-2.8mmol/L of calcium, 1.2-1.8mmol/L of magnesium, 1.2-1.8mmol/L of sulfur, 60-80 mu mol/L of iron, 52-58 mu mol/L of boron, 6-8 mu mol/L of manganese, 0.8-0.9 mu mol/L of zinc, 0.12-0.18 mu mol/L of copper, 0.06-0.1 mu mol/L of molybdenum, 805.2-5.8g/L of polysorbate-890.25 g/L of organic silicon and 5-15mg/L of organic chelated titanium;
when the fertilizer is used for root system dressing or seed soaking treatment, the fertilizer comprises the following components: 20-25mmol/L of gamma-aminobutyric acid, 1.2-1.4mmol/L of phosphorus, 6.5-7.5mmol/L of potassium, 3.5-4.5mmol/L of calcium, 2.2-2.8mmol/L of magnesium, 1.2-1.8mmol/L of sulfur, 60-80 mu mol/L of iron, 65-75 mu mol/L of boron, 12-15 mu mol/L of manganese, 1.2-1.4 mu mol/L of zinc, 0.22-0.28 mu mol/L of copper, 0.1-0.2 mu mol/L of molybdenum, 805.2-5.8g/L of polysorbate-890.25 g/L of organic silicon and 5-15mg/L of organic chelated titanium.
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