CN107581153A - A kind of method of black bottom soft-shelled turtle ecologic breeding - Google Patents
A kind of method of black bottom soft-shelled turtle ecologic breeding Download PDFInfo
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Abstract
The invention belongs to cultural technique field, and in particular to a kind of method of black bottom soft-shelled turtle ecologic breeding, it is characterised in that：(1) soft-shelled turtle pond is built；(2) young soft-shelled turtle aquaculture management；(3) marketable turtle culture management；(4) daily management：Periodically carry out water quality cleaning, observation sterilizes Routine Management into soft-shelled turtle situation, soft-shelled turtle pond；(5) capture.Advantage：Ecology is cultivated, and ensures the quality of black bottom soft-shelled turtle, and the black bottom soft-shelled turtle for cultivating out is strong, survival rate is high.
Description
【Technical field】
The invention belongs to cultural technique field, and in particular to a kind of method of black bottom soft-shelled turtle ecologic breeding.
【Background technology】
Pingnan County ink bottom soft-shelled turtle, it is one of the high-quality freshwater fish in Guangxi, (agricultural product are geographical to be marked Chinese geography famous special product
Will).Originate in Guangxi Pingnan County Yu Jiang basins.From in the early 1990s, rivers fish wild seed is collected by some peasant households of Guangxi Pingnan County
Propagated artificially, then carry out artificial propagation success.The kind has good dressed fish height, meat, delicious flavour, nutritive value
The features such as high, it is well received by consumers, wide market.Beginning at the beginning of 21 century, Guangxi Pingnan County are focused on guiding vast soft-shelled turtle
Agriculture cultivates to cultivate to famous-particular-excellent kind from traditional category to be changed, and in December, 2005, fishery cultivating branch in Pingnan County set up, and branch will
Scattered raiser is concentrated in the form of member system, is managed collectively, and is in planning and designing, cultural technique, market information etc.
Member provides service.268 hectares of Terrapin raising area in 2006, based on black bottom soft-shelled turtle, black bottom soft-shelled turtle cultured area reaches breed variety
To 138 hectares, 280 tons of annual production.
But because mankind's activity is more and more, living environment, water quality are also increasingly severeer, and black bottom soft-shelled turtle is propagated artificially
Meat has change after more generation breedings so that and the price reduction of black bottom soft-shelled turtle, economic benefit can not improve, therefore in order to keep ink
The quality of bottom soft-shelled turtle is, it is necessary to which a kind of mode of ecologic breeding is raised.
【The content of the invention】
The goal of the invention of the present invention is：It is bionical it is an object of the invention to provide a kind of method of black bottom soft-shelled turtle ecologic breeding
State is cultivated, and ensures the quality of black bottom soft-shelled turtle, and the black bottom soft-shelled turtle for cultivating out is strong, survival rate is high.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of method of black bottom soft-shelled turtle ecologic breeding：Comprise the following steps：
(1) soft-shelled turtle pond is built：Environment is quiet, it is pollution-free to have abundant water resources, and water power has a good transport service, intakes draining easily
Fang Jianchi；Soft-shelled turtle pond position is leeward, and side is high 2 meters on the sunny side, and medial surface builds the ceramic tile of Smooth, and one layer of goose ovum is first laid in soft-shelled turtle bottom of pond portion
The fine sand of 2-5 centimetres of stone and then layer overlay thickness, solarization back of the body platform is also set up in soft-shelled turtle pond, is disappeared before launching young soft-shelled turtle by soft-shelled turtle pond
Poison；
(2) young soft-shelled turtle aquaculture management：The throw-in density of young soft-shelled turtle is 10/m2, the feed containing Chinese herbal and crude drugs preparations is launched daily
0.2-0.3kg/ only, is divided into and launched three times for one day, and launch 30% in the morning, launches 40% afternoon, launches 30% at night, keeps water temperature
22 ± 2 DEG C, water pH is 7.0 ± 0.2；
(3) marketable turtle culture management：Soft-shelled turtle pond is put into after into soft-shelled turtle, also sets up a laying eggs room into soft-shelled turtle pond, there is any discrepancy for spawning setting
Mouthful, laid inside fine sand, the density launched into soft-shelled turtle pond is 2/m2, the feed 0.1- containing Chinese herbal and crude drugs preparations is launched daily
0.2kg/, 0.02-0.05kg/, pasture and water；
(4) daily management：Periodically carry out water quality cleaning, observation sterilizes Routine Management into soft-shelled turtle situation, soft-shelled turtle pond；
(5) capture：Capture a few days ago stops feeding, the draining of soft-shelled turtle pond, is chosen as soft-shelled turtle seizure, it is clean rear every that clear water is flushed into soft-shelled turtle
Only use woven bags packaging；
The feed containing Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：Rice bran 50-60
Part, fish meal 30-40 parts, premix 5-10 parts, soya-bean oil 3-5 parts, fresh and alive yellow meal worm 3-5 parts, salt 0.1-0.5 parts, zeolite powder 1-
5 parts, Chinese herbal and crude drugs preparations 10-15 parts；
The Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：40 parts -80 parts of frankincense, caulis piperis futokadsurae
30 parts -50 parts, 30 parts -50 parts of kelp, 30 parts -40 parts of the Radix Astragali, 40 parts -50 parts of the fleece-flower root, 20 parts -30 parts of Chinese cassia tree, aloe 20
Parts -35 parts, 30 parts -40 parts of hawthorn, 15 parts -30 parts of Semen Cuscutae, 10 parts -15 parts of caraway seed, 10 parts -20 parts of dark plum, 10 parts of jujube -
20 parts, windproof 5 parts -15 parts, 10 parts -25 parts of radix glycyrrhizae.
Preferably, Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：50 parts -70 parts of frankincense, sea
35 parts -45 parts of wind rattan, 35 parts -40 parts of kelp, 33 parts -38 parts of the Radix Astragali, 42 parts -48 parts of the fleece-flower root, 23 parts -27 parts of Chinese cassia tree, aloe
25 parts -30 parts, 32 parts -37 parts of hawthorn, 20 parts -27 parts of Semen Cuscutae, 11 parts -14 parts of caraway seed, 12 parts -18 parts of dark plum, jujube 12
Part -18 parts, windproof 8 parts -12 parts, 15 parts -20 parts of radix glycyrrhizae.
More preferred, Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：60 parts of frankincense, sea wind
40 parts of rattan, 37 parts of kelp, 35 parts of the Radix Astragali, 45 parts of the fleece-flower root, 25 parts of Chinese cassia tree, 28 parts of aloe, 35 parts of hawthorn, 25 parts of Semen Cuscutae, amaranth
12 parts of wormwood artemisia seed, 14 parts of dark plum, 15 parts of jujube, windproof 10 parts, 18 parts of radix glycyrrhizae.
Further illustrate, the mode of the sterilization be soft-shelled turtle pond is put 4/5 water, then addition sterilization hydroful soft-shelled turtle pond, immersion
1-2 days, rinsed 3 times using clear water after draining.
Further illustrate, thimerosal used in sterilization is prepared from the following materials：Liquor potassic permanganate 10-20g/l,
Shrub althea flower extract solution 15-35g/l, Sophora alopecuroide extract solution 20-30g/l, madder extract solution 30-40g/l.
Further illustrate, the shrub althea flower extract solution is will to dry after shrub althea flower addition diatomite is pulverized to add quality
After concentration is 75% alcohol solution dipping 5-10 minute, backflow steaming is carried out under the conditions of pressure is 0.1MPa, temperature is 35 DEG C
10-15 minutes are evaporated, it is shrub althea flower extract solution then to carry out press filtration and obtain filtrate.
Further illustrate, the Sophora alopecuroide extract solution is to add 5 times of volume of water after fresh Sophora alopecuroide is smashed to pieces to carry out rope
Family name extracts, and obtains filtrate, adds mass concentration and is carried to be centrifuged after 75% ethanol solution alcohol extracting, taking filtrate to can obtain Sophora alopecuroide
Take liquid.
Further illustrate, the madder extract solution is by herb 3 times of volume of water of addition filter to take after decocting 30 minutes
Filtrate, 1.21 times of original volume are then concentrated into, add after the ethanol solution alcohol extracting that mass concentration is 85% and centrifuge, take filtrate i.e.
It can obtain madder extract solution.
Further instruction, frankincense are that water-bath adds at 30 DEG C by 100g frankincenses addition 200ml 75wt% ethanol solutions
Hot Soxhlet refluxing extraction 1-2 hours, go filtrate.
Further instruction, caulis piperis futokadsurae are that the 100g caulis piperis futokadsurae stems dried are pulverized and sieved by micronizer
0.2-0.5 microns are selected, the 90wt% ethanol solutions for adding 200ml are extracted, and recycling design obtains alcohol-extracted extract, will
Medicinal extract obtains the ethyl acetate extract of caulis piperis futokadsurae after being extracted with ethyl acetate, stand-by.
Further instruction, kelp are by after cold water immersion 30-40 minutes of the kelp using 2 times of volumes, rinse 3 kelps
Afterwards, the water heating for adding 3-5 times of volume decocts 30-40 minutes, filters to take filtrate, continues to decoct mistake after 30-40 minutes by the dregs of a decoction
Leaching filter residue, merge filtrate twice, after ultra micro membrane filtration, spray drying obtains powdered extract, stand-by.
Further instruction, the Radix Astragali be after the Radix Astragali is crushed according to volume ratio be 1:2, which add 80wt% ethanol solutions, is carried out
10-15 minutes are extracted, using microwave radiation exaraction, filtrate is filtered to take, filter residue is repeated the above steps 2-3 times, merging filtrate is
It can obtain Radix Astragali extractive solution.
Further instruction, the fleece-flower root are to add 5-10 times of water after 100g fleece-flower root root block is cut into slices to be decocted
Filtrate is filtered to take after 30 minutes, it is stand-by.
Further instruction, Chinese cassia tree are that 100g Chinese cassia tree crushed into 100 mesh sieves, are subsequently added into 5-10 times of material quantity
85wt% ethanol solutions carry out extracting 10-15 minutes, are extracted using ultrasonic assistant, filter to take filtrate.
Further instruction, aloe are that the 85wt% ethanol solutions of 5-10 times of addition after 100g fresh aloe is smashed to pieces enter
Row extraction 10-15 minutes, extracted using ultrasonic assistant, filter to take filtrate, it is stand-by.
Further instruction, hawthorn are the 85wt% ethanol solutions of 5-10 times of addition after 100g dry Fructus Crataegi is crushed
Carry out extracting 10-15 minutes, extracted using ultrasonic assistant, filter to take filtrate, powder is obtained after vacuum drying, is then used
Chloroform is extracted, take chloroform layer be concentrated under reduced pressure dry after obtain haw thorn extract, it is stand-by.
Further instruction, Semen Cuscutae are that the 85wt% ethanol solutions that 100g Semen Cuscutae tendril is added to 3 times of volumes carry
1-2 hours are taken, filter to take filtrate, filter residue repeats to extract, and merges the filtrate of 3 times, you can obtain extracting solution of south dodder seed.
Further instruction, caraway seed are the 85wt% ethanol solutions that 3 times of volumes are added after 100g caraway seed is crushed
1-2 hours are extracted, filter to take filtrate, filter residue repeats to extract, and merges the filtrate of 3 times, you can obtain caraway seed extract solution.
Further instruction, dark plum are that the 85wt% ethanol solutions of 5-10 times of addition after 100g dry dark plum is crushed are carried out
10-15 minutes are extracted, using microwave radiation exaraction, filtrate is filtered to take, powder is obtained after vacuum drying, is then entered using chloroform
Row extraction, take chloroform layer be concentrated under reduced pressure dry after obtain smoked plum extractive, it is stand-by.
Further instruction, jujube are that 100g jujube crushed 100 mesh sieves, are subsequently added into 3-5 times of 85wt% of material quantity
Ethanol solution carries out extracting 10-15 minutes, is extracted using ultrasonic assistant, filters to take filtrate.
Further instruction, windproof is that the 85wt% ethanol solutions that 100g windproof herb is added to 3 times of volumes extract 1-2
Hour, filtrate is filtered to take, filter residue repeats to extract, and merges the filtrate of 3 times, you can obtain windproof extract solution.
Further instruction, radix glycyrrhizae are small fire decoctions after the water immersion 10-15 minutes that 100g dry herb is added into 10 times
40-50 minutes can be filtrated to get licorice extract, stand-by.
The drug effect of above-mentioned Chinese medicine is as follows：
Frankincense is pungent, bitter, temperature.The thoughts of returning home, liver, the spleen channel.Containing boswellic acid, mastic hydrocarbon, frankincense hypo acid, masticinic acid etc..Separately
Oil containing performance, predominantly firpene；Relieved pain with blood-activating and qi-promoting, detumescence and promoting granulation.For chest impediment and cardialgia, gastral cavity pain, dysmenorrhoea Amenorrhea,
Postpartum stasis, abdominal mass stomachache, arthralgia pain due to rheumatism, muscle arteries and veins contraction, traumatic injury, carbuncle swells sore.
Caulis piperis futokadsurae Chinese medicine name.For Piperaceae plant wind rattan Piper kadsura (Choisy) 0hwi drying rattan.Summer,
Qiu Erji is tapped, and is removed root, leaf, is dried.Acrid flavour, hardship, slightly warm in nature.Return liver warp.Wind-damp dispelling, degrading the channel, stopping numbness pain.For chill
Arthritis with fixed pain caused by dampness, podomere pain, muscle arteries and veins contraction, joint stuffiness.
Kelp (Ecklonia kurome) is also known as " black dish ", " goose slaps dish ", " five palm dishes " etc..Phaeophyceae, Alariaceae.Spore
Daughter is large-scale, brown, keratin, high 30-100cm, leaflet piece, shank, holdfast, holdfast rhizoid shape.The V shape branch of rhizoid two,
Shank cylindrical shape, nearly blade part is gradually flat, and blade both sides pinniform or coverts shape branch, middle part is slightly thick, intercalary growth, long 1-
12cm, thick 3-7mm, rough sawn tingia edge.Zoospore is born in blade two sides.Have and do not wait the alternation of generations significantly.It is grown on temperate zone
In ocean.Zhejiang Province, China, ALONG COASTAL FUJIAN are distributed.Nature and flavor are trembled with fear, salty.Return liver, stomach, kidney channel.There are softening and resolving hard mass, detumescencing diuresis, profit
The work(of lower dissolving phlegm, clinically for goitre, lymphonodi cervicales is swollen, bronchitis, pulmonary tuberculosis, cough and cataract of old people
Deng also trying out in treating cancer.
The Radix Astragali, also known as continuous stilbene.Nature and flavor：It is sweet, tepor.Channel tropism：Return lung, spleen, liver and kidney channel.Perennial herb, high 50-100
Centimetre.Main root is plump, wooden, normal branch, canescence.Stem is upright, top multi-branched, there is thin rib, by white pubescence.Perennial grass
This, it is high 50-100 centimetres.Originate in the ground such as the Inner Mongol, Shanxi, Gansu, Heilungkiang.The medicinal of the Radix Astragali has more than 2000 years so far
History, it has enhancing body's immunity, liver protection, diuresis, anti-aging, resisting stress, decompression and wide antibacterial action.But table
Real evil to contain, retention of dampness due to stagnation of QI, dyspepsia is stagnated, ulcer from the beginning of or heat toxin such as still contains at real example, and hyperactivity of yang due to yin deficiency person, the Jun Xujin after bursting
Clothes.
The fleece-flower root (scientific name：Fallopia multiflora (Thunb.) Harald.), also known as spend more knotweed, purple black rattan, night
Hand over rattan etc..It is that the polygonaceae knotweed race fleece-flower root belongs to perennial winding liana, root tuber is plump, oblong, dark brown.Raw mountain valley fills
Clump, hillside sylvan life, ditch curb gap.Produce Southern Shaanxi, SOUTH OF GANSU, East China, Central China, south China, Sichuan, Yunnan and Guizhou.Bitter sweet
It is puckery, tepor.Sweet, temperature is nontoxic.Its root tuber is used as medicine, and can calm the nerves, be blood-nourishing, active, and removing toxic substances (preventing malaria), disappear carbuncle；RADIX POLYGONI MULTIFLORI PREPARATA can help
Essence and blood, black beard and hair, strengthening the bones and muscles, filling liver kidney, it is your common detail medicinal material.
Chinese cassia tree (Classification system：Cinnamomum cassia Presl), also known as beautiful osmanthus, male osmanthus, cajaput, great Gui, peppery osmanthus,
Safety tree, cassia, it is the dry bark of canella Chinese cassia tree.Bark fragrance, spices can be made, taste is with originating from Sri Lanka's meat
The cassia bark in osmanthus is similar, but pepperyyer, delicious not as good as cassia bark, and thick compared with cassia bark.It is pungent, sweet, heat.Return kidney, spleen, the heart, Liver Channel.Fire is mended to help
Sun, eliminating cold to stop pain, warming meridian.For the insufficiency of the kidney yang, positive fistula Gong Leng, the cold abdomen of gastral cavity abdomen, arthritis pain in the back, cold hernia stomachache, blood coagulation of trembling with fear
The stasis of blood, closed dysmenorrhea, chest impediment and cardialgia.
Aloe (scientific name：Aloe vera) Aloe is Liliaceae perennial evergreen herbaceous plant, leafage is raw, big and fertile
Thickness, in seat shape or stem top is born in, there is sharp tooth thorn at the normal lanceolar of leaf or the short width of leaf, edge.Inflorescence is umbrella shape, total shape, spike, circle
Taper etc., color is in red, yellow or tool redness of the skin or complexion spot, six, petal, six pieces of gynoecium.The more joints of perianth base portion are into tubular.Nature and flavor：Hardship,
It is cold.Removing heat from the liver, defaecation.For：Constipation, infantile malnutrition, infantile convulsion；Wet tinea is controlled outside.
Hawthorn, Chinese medicine name.For rosaceous plant large-fruited Chinese hawthorn Crataegus pin, natiida
Bge.var.majorN.E.Br. or hawthorn Crataegus pin.nati fida Bge. dry mature fruit.Autumn fruit
Harvest, cut into slices when ripe, dry.It is sour, sweet, tepor.Returns spleen, stomach, Liver Channel.Food digesting stomach fortifying, scattered stasis, change turbid lipid-loweringing.For
Meat stagnation, gastral cavilty turgor, diarrhea dysentery abdominal pain, blood stasis closed, postpartum stasis, confidant sting, chest impediment and cardialgia, hernia pain, high fat
Mass formed by blood stasis.Charred FRUCTUS CRATAEGI promoting digestion effect enhancing.It is not well for meat stagnation, rush down dysentery.
Semen Cuscutae (scientific name：Cuscuta chinensis Lam.), alias abdicates true, beans parasitism, beans King of Hell, yellow silk, yellow silk
Rattan, reticulate millettia, spun gold rattan etc..Annual parasitic draft.Stem is wound, and yellow is very thin, no leaf.Inflorescence side is given birth to, few flower or more flowers
Generate small umbrella shape or small glomerule；Bract and squamella are small, flakey；Bennet is slightly sturdy；Calyx cup-shaped, connect below middle part
Close, sliver triangular shape；Corolla white, kettle shape；Stamen the raw micro- lower place of corolla lobe sinus；Scale Long Circle；Ovary is subsphaeroidal,
Style 2.Capsule is spherical, is almost all the corolla harbored and is surrounded.Seed 2-49, it is filbert, it is avette, 1 millimeter is about, surface is thick
It is rough.It is sweet, warm, return kidney,liver,spleen to pass through, there is the effect of nourishing liver and kidney, astringent sperm contracting urine, antiabortive, improving eyesight, antidiarrheal, begin to carry《Legendary god of farming's sheet
Grass warp》, it is listed in top grade.Sugariness typically has a tonic effect, sweet pungent tepor, reports gas and neutralizes, both can yang-tonifying, again can benefit it is cloudy, tool
Have it is warm but not dry, mend without it is stagnant the characteristics of.Controlling nocturnal emission with astringent drugs is antiabortive and gonadotropic Effect.The traditional Chinese medical science thinks that the kidney being the origin of congenital constitution, interior residence member
Cloudy and Yuanyang, is the root of human body reproductive development, the motive power of internal organs functional activity.
A kind of fruit of wild plant of caraway seed, autumn smoothes out with the fingers come after dry and wear into powder just into wormwood artemisia face.It is special that wormwood artemisia seed has
Smell, wormwood artemisia face has viscosity after meeting water, and wormwood artemisia seed, which has, promotees digestion, stomach invigorating, heat-clearing and other effects.
Dark plum Chinese medicine name.It is sour, puckery, it is mild-natured.Return liver, spleen, lung, large intestine channel.For rose department plant plum Prunus mume
(Sieb.) Sieb.etZuce. drying almost ripe fruit.There is cultivation China various regions, most with each province on the south the Yangtze river basin.
With astringing the lung, puckery intestines, promote the production of body fluid, the effect of claming ascaris.It is usually used in chronic cough of deficiency lung, protracted dysentery and diarrhea, abnormal heat is quenched one's thirst, ascariasis of biliary tract vomiting stomachache.
Jujube, also known as jujube, belong to Angiospermae, Dicotyledoneae, Rhamnales, Rhamnaceae, the plant of zizyphus.Its
Vitamin content is very high, there is the good reputation of " natural complex ball ", has nourishment for vitality, the effect of enriching blood.It is sweet, temperature.Returns spleen, stomach
Through.Tonifying middle-Jiao and Qi, nourishing blood and tranquilization, relax the property of medicine.For weakness of the spleen and the stomach, anorexia and loose stool, blood deficiency chlorosis, women's hysteria.
Windproof Chinese medicine name.For labiate Indian Epimeredi Herb Anisomeles indica (L.) O.Ktze herb.It is distributed in
Jiangxi, Zhejiang, Fujian, Taiwan, Hunan, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan and Tibet.With wind-dispelling, dehumidifying, detoxify it
Effect.It is usually used in body heat of catching a cold, vomits, stomachache, arthralgia and myalgia, sore, eczema, haemorrhoids.Acrid flavour, hardship, it is warm-natured.Return bladder, liver,
Kidney channel.
Radix glycyrrhizae (scientific name：Glycyrrhiza uralensis Fisch) alias：State is old, Herba Hedyotis cantonensis, Glycyrrhiza Uralensis, sweet tea root
Son.Pulse family, Glycyrrhiza perennial herb, root is sturdy with root-like stock, is a kind of help Chinese herbal medicine.To a kind of human body medicine well,
Medicinal part is root and rhizome, and Medicinal Materials Characters root is in cylinder, long 25~100 centimetres, 0.6~3.5 centimetre of diameter.Crust is elastic
Differ, surface rufous or taupe brown.For rhizome in cylinder, there is bud scar on surface, has marrow in the middle part of section.Gas is micro-, sweet and special.
Major function is clearing heat and detoxicating, expelling phlegm and arresting coughing, gastral cavity abdomen etc..Happiness is dark moist, the low dry climate of the long temperature of sunshine.Radix glycyrrhizae grows more
In arid, semiarid desert steppe, desert edge and loess hill area.Root and root-like stock hyoscine.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
The application Ecology carries out black bottom soft-shelled turtle cultivation, can sufficiently meet the growing space of black bottom soft-shelled turtle, and the feeding fed
Not only the power element containing basal feed also with addition of Chinese medicine preparation and fresh and alive food, can promote the feed intake of black bottom soft-shelled turtle, carry material
The immunity activity of high black bottom soft-shelled turtle, and then improve the survival rate of black bottom soft-shelled turtle.
The Chinese medicine preparation designed in the present invention is the Chinese medicine principle according to monarch, by frankincense, caulis piperis futokadsurae, kelp,
It is monarch drug in a prescription altogether that the Radix Astragali, fleece-flower root collective effect, which have the effects such as myogenic, removing toxic substances, channels and collaterals, protect liver, antibacterial, Chinese cassia tree, aloe, hawthorn,
Semen Cuscutae collective effect have the function that to promote blood circulation dissolving stasis, help digestion, improving eyesight be altogether as ministerial drug, it is caraway seed, dark plum, jujube, windproof
Collective effect has the function that stomach invigorating, food calling, clearing damp are that radix glycyrrhizae is clearing heat and detoxicating as adjutant altogether, and neutralizing the property of medicine and being used as makes medicine；
14 taste medicine collective effects, complement each other, existing cold and cool product in side, also there is a medicine of warm nature, using medicines of both cold and hot natures simultaneously, existing antibacterial, anti-inflammatory,
Anti-oxidant medicine, also there are scattered wind, the product of QI invigorating, mutually assistant system, cooperate, the prescription compatibility is proper, and all medicines play reinforcement altogether
The effect of matter, and therapeutic effect is notable, can be achieved to treat both principal and secondary aspect of disease, it is securely and reliably, without any side effects.
【Embodiment】
In order to facilitate the understanding of the purposes, features and advantages of the present invention, with reference to specific embodiment pair
The embodiment of the present invention is described in detail.Many details are elaborated in the following description in order to fully manage
The solution present invention.But the invention can be embodied in many other ways as described herein, those skilled in the art can
To do similar improvement in the case of without prejudice to intension of the present invention, therefore the present invention is not limited by following public specific implementation
System.
The preparation of raw material：
Frankincense be by 100g frankincenses add 200ml 75wt% ethanol solutions at 30 DEG C heating water bath Soxhlet refluxing extraction
1-2 hours, go filtrate.
Caulis piperis futokadsurae is by micronizer pulverize and sieve by the caulis piperis futokadsurae stem that 100g is dried selecting 0.2-0.5 micro-
Rice, the 90wt% ethanol solutions for adding 200ml are extracted, and recycling design obtains alcohol-extracted extract, by medicinal extract ethyl acetate
The ethyl acetate extract of caulis piperis futokadsurae is obtained after extraction, it is stand-by.
Kelp is by after cold water immersion 30-40 minutes of the kelp using 2 times of volumes, after rinsing 3 kelps, adds 3-5 times
The water heating of volume decocts 30-40 minutes, filters to take filtrate, and the dregs of a decoction are continued to filter to take filter residue after decocting 30-40 minutes, merged
Filtrate twice, after ultra micro membrane filtration, spray drying obtains powdered extract, stand-by.
The Radix Astragali be after the Radix Astragali is crushed according to volume ratio be 1:2 addition 80wt% ethanol solutions carry out extracting 10-15 minutes,
Using microwave radiation exaraction, filtrate is filtered to take, filter residue is repeated the above steps 2-3 times, merging filtrate can obtain astragalus extraction
Liquid.
The fleece-flower root is to add 5-10 times of water after 100g fleece-flower root root block is cut into slices filter after decocting 30 minutes
Filtrate is taken, it is stand-by.
Chinese cassia tree is that 100g Chinese cassia tree crushed into 100 mesh sieves, is subsequently added into 5-10 times of 85wt% ethanol solution of material quantity and enters
Row extraction 10-15 minutes, extracted using ultrasonic assistant, filter to take filtrate.
Aloe is that the 85wt% ethanol solutions of 5-10 times of addition after 100g fresh aloe is smashed to pieces carry out extracting 10-15 points
Clock, extracted using ultrasonic assistant, filter to take filtrate, it is stand-by.
Hawthorn is that the 85wt% ethanol solutions of 5-10 times of addition after 100g dry Fructus Crataegi is crushed carry out extraction 10-15
Minute, extracted using ultrasonic assistant, filter to take filtrate, powder is obtained after vacuum drying, is then extracted using chloroform, is taken
Chloroform layer be concentrated under reduced pressure dry after obtain haw thorn extract, it is stand-by.
Semen Cuscutae is that the 85wt% ethanol solutions that 100g Semen Cuscutae tendril is added to 3 times of volumes extract 1-2 hours, filtering
Filtrate is taken, filter residue repeats to extract, and merges the filtrate of 3 times, you can obtain extracting solution of south dodder seed.
Caraway seed is that the 85wt% ethanol solutions that 3 times of volumes are added after 100g caraway seed is crushed extract 1-2 hours, mistake
Leaching filtrate, filter residue repeat to extract, and merge the filtrate of 3 times, you can obtain caraway seed extract solution.
Dark plum is that the 85wt% ethanol solutions of 5-10 times of addition after 100g dry dark plum is crushed carry out extracting 10-15 points
Clock, using microwave radiation exaraction, filtrate is filtered to take, powder is obtained after vacuum drying, is then extracted using chloroform, takes chloroform
Layer be concentrated under reduced pressure dry after obtain smoked plum extractive, it is stand-by.
Jujube is that 100g jujube crushed 100 mesh sieves, is subsequently added into 3-5 times of 85wt% ethanol solution of material quantity and is carried
10-15 minutes are taken, are extracted using ultrasonic assistant, filter to take filtrate.
Windproof is that the 85wt% ethanol solutions that 100g windproof herb is added to 3 times of volumes extract 1-2 hours, filters to take filter
Liquid, filter residue repeat to extract, and merge the filtrate of 3 times, you can obtain windproof extract solution.
Radix glycyrrhizae is small fire decoction 40-50 minutes after the water immersion 10-15 minutes that 100g dry herb is added into 10 times
Licorice extract is filtrated to get, it is stand-by.
Shrub althea flower will be dried and add the alcohol solution dipping 5-10 that addition mass concentration is 75% after diatomite is pulverized
After minute, cohobation 10-15 minutes are carried out under the conditions of pressure is 0.1MPa, temperature is 35 DEG C, press filtration is then carried out and obtains
Filtrate is shrub althea flower extract solution.
5 times of volume of water are added after fresh Sophora alopecuroide is smashed to pieces and carry out surname extraction, obtain filtrate, adding mass concentration is
Centrifuged after 75% ethanol solution alcohol extracting, take filtrate to can obtain Sophora alopecuroide extract solution.
Herb 3 times of volume of water of addition are carried out filtering to take filtrate after decocting 30 minutes, are then concentrated into the 1.21 of original volume
Times, mass concentration is added to be centrifuged after 85% ethanol solution alcohol extracting, taking filtrate to can obtain madder extract solution.
Embodiment 1：
A kind of method of black bottom soft-shelled turtle ecologic breeding：Comprise the following steps：
(1) soft-shelled turtle pond is built：Environment is quiet, it is pollution-free to have abundant water resources, and water power has a good transport service, intakes draining easily
Fang Jianchi；Soft-shelled turtle pond position is leeward, and side is high 2 meters on the sunny side, and medial surface builds the ceramic tile of Smooth, and one layer of goose ovum is first laid in soft-shelled turtle bottom of pond portion
The fine sand of 2 centimetres of stone and then layer overlay thickness, solarization back of the body platform is also set up in soft-shelled turtle pond, is carried out disinfection before launching young soft-shelled turtle by soft-shelled turtle pond；
The mode of the sterilization be soft-shelled turtle pond is put 4/5 water, then addition sterilization hydroful soft-shelled turtle pond, soaks 1-2 days, after draining
Rinsed 3 times using clear water；
Used thimerosal is prepared from the following materials：Liquor potassic permanganate 10g/l, shrub althea flower extract solution 15g/l,
Sophora alopecuroide extract solution 20g/l, madder extract solution 30g/l；
(2) young soft-shelled turtle aquaculture management：The throw-in density of young soft-shelled turtle is 10/m2, the feed containing Chinese herbal and crude drugs preparations is launched daily
0.2kg/ only, is divided into and launched three times for one day, and launch 30% in the morning, launches 40% afternoon, launches 30% at night, keeps water temperature 22
± 2 DEG C, water pH is 7.0 ± 0.2；
(3) marketable turtle culture management：Soft-shelled turtle pond is put into after into soft-shelled turtle, also sets up a laying eggs room into soft-shelled turtle pond, there is any discrepancy for spawning setting
Mouthful, laid inside fine sand, the density launched into soft-shelled turtle pond is 2/m2, the feed 0.1kg/ containing Chinese herbal and crude drugs preparations is launched daily
Only, pasture and water 0.02kg/；
(4) daily management：Periodically carry out water quality cleaning, observation sterilizes Routine Management into soft-shelled turtle situation, soft-shelled turtle pond；
(5) capture：Capture a few days ago stops feeding, the draining of soft-shelled turtle pond, is chosen as soft-shelled turtle seizure, it is clean rear every that clear water is flushed into soft-shelled turtle
Only use woven bags packaging；
The feed containing Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：50 parts of rice bran,
30 parts of fish meal, 5 parts of premix, 3 parts of soya-bean oil, 3 parts of fresh and alive yellow meal worm, 0.1 part of salt, 1 part of zeolite powder, 10 parts of Chinese herbal and crude drugs preparations；
The Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：40 parts of frankincense, 30 parts of caulis piperis futokadsurae,
30 parts of kelp, 30 parts of the Radix Astragali, 40 parts of the fleece-flower root, 20 parts of Chinese cassia tree, 20 parts of aloe, 30 parts of hawthorn, 15 parts of Semen Cuscutae, caraway seed 10
Part, 10 parts of dark plum, 10 parts of jujube, windproof 5 parts, 10 parts of radix glycyrrhizae.
Embodiment 2：
A kind of method of black bottom soft-shelled turtle ecologic breeding：Comprise the following steps：
(1) soft-shelled turtle pond is built：Environment is quiet, it is pollution-free to have abundant water resources, and water power has a good transport service, intakes draining easily
Fang Jianchi；Soft-shelled turtle pond position is leeward, and side is high 2 meters on the sunny side, and medial surface builds the ceramic tile of Smooth, and one layer of goose ovum is first laid in soft-shelled turtle bottom of pond portion
The fine sand of 5 centimetres of stone and then layer overlay thickness, solarization back of the body platform is also set up in soft-shelled turtle pond, is carried out disinfection before launching young soft-shelled turtle by soft-shelled turtle pond；
The mode of the sterilization be soft-shelled turtle pond is put 4/5 water, then addition sterilization hydroful soft-shelled turtle pond, soaks 2 days, makes after draining
Rinsed 3 times with clear water；
Used thimerosal is prepared from the following materials：Liquor potassic permanganate 20g/l, shrub althea flower extract solution 35g/l,
Sophora alopecuroide extract solution 30g/l, madder extract solution 40g/l；
(2) young soft-shelled turtle aquaculture management：The throw-in density of young soft-shelled turtle is 10/m2, the feed containing Chinese herbal and crude drugs preparations is launched daily
0.3kg/ only, is divided into and launched three times for one day, and launch 30% in the morning, launches 40% afternoon, launches 30% at night, keeps water temperature 22
DEG C, water pH is 7.0；
(3) marketable turtle culture management：Soft-shelled turtle pond is put into after into soft-shelled turtle, also sets up a laying eggs room into soft-shelled turtle pond, there is any discrepancy for spawning setting
Mouthful, laid inside fine sand, the density launched into soft-shelled turtle pond is 2/m2, the feed 0.2kg/ containing Chinese herbal and crude drugs preparations is launched daily
Only, pasture and water 0.02-0.05kg/；
(4) daily management：Periodically carry out water quality cleaning, observation sterilizes Routine Management into soft-shelled turtle situation, soft-shelled turtle pond；
(5) capture：Capture a few days ago stops feeding, the draining of soft-shelled turtle pond, is chosen as soft-shelled turtle seizure, it is clean rear every that clear water is flushed into soft-shelled turtle
Only use woven bags packaging；
The feed containing Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：60 parts of rice bran,
40 parts of fish meal, 10 parts of premix, 5 parts of soya-bean oil, 5 parts of fresh and alive yellow meal worm, 0.5 part of salt, 5 parts of zeolite powder, Chinese herbal and crude drugs preparations 15
Part；
The Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：In parts by weight include with
Lower raw material is prepared：80 parts of frankincense, 50 parts of caulis piperis futokadsurae, 50 parts of kelp, 40 parts of the Radix Astragali, 50 parts of the fleece-flower root, 30 parts of Chinese cassia tree, aloe
35 parts, 40 parts of hawthorn, 30 parts of Semen Cuscutae, 15 parts of caraway seed, 20 parts of dark plum, 20 parts of jujube, windproof 15 parts, 25 parts of radix glycyrrhizae.
Embodiment 3：
A kind of method of black bottom soft-shelled turtle ecologic breeding：Comprise the following steps：
(1) soft-shelled turtle pond is built：Environment is quiet, it is pollution-free to have abundant water resources, and water power has a good transport service, intakes draining easily
Fang Jianchi；Soft-shelled turtle pond position is leeward, and side is high 2 meters on the sunny side, and medial surface builds the ceramic tile of Smooth, and one layer of goose ovum is first laid in soft-shelled turtle bottom of pond portion
The fine sand of 3 centimetres of stone and then layer overlay thickness, solarization back of the body platform is also set up in soft-shelled turtle pond, is carried out disinfection before launching young soft-shelled turtle by soft-shelled turtle pond；
The mode of the sterilization be soft-shelled turtle pond is put 4/5 water, then addition sterilization hydroful soft-shelled turtle pond, soaks 1 day, makes after draining
Rinsed 3 times with clear water；
Used thimerosal is prepared from the following materials：Liquor potassic permanganate 12g/l, shrub althea flower extract solution 20g/l,
Sophora alopecuroide extract solution 22g/l, madder extract solution 32g/l；
(2) young soft-shelled turtle aquaculture management：The throw-in density of young soft-shelled turtle is 10/m2, the feed containing Chinese herbal and crude drugs preparations is launched daily
0.2kg/ only, is divided into and launched three times for one day, and launch 30% in the morning, launches 40% afternoon, launches 30% at night, keeps water temperature 24
DEG C, water pH is 7.2；
(3) marketable turtle culture management：Soft-shelled turtle pond is put into after into soft-shelled turtle, also sets up a laying eggs room into soft-shelled turtle pond, there is any discrepancy for spawning setting
Mouthful, laid inside fine sand, the density launched into soft-shelled turtle pond is 2/m2, the feed 0.2kg/ containing Chinese herbal and crude drugs preparations is launched daily
Only, pasture and water 0.03kg/；
(4) daily management：Periodically carry out water quality cleaning, observation sterilizes Routine Management into soft-shelled turtle situation, soft-shelled turtle pond；
(5) capture：Capture a few days ago stops feeding, the draining of soft-shelled turtle pond, is chosen as soft-shelled turtle seizure, it is clean rear every that clear water is flushed into soft-shelled turtle
Only use woven bags packaging；
The feed containing Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：Rice bran 50-60
Part, 35 parts of fish meal, 7 parts of premix, 4 parts of soya-bean oil, 3 parts of fresh and alive yellow meal worm, 0.3 part of salt, 3 parts of zeolite powder, Chinese herbal and crude drugs preparations 11
Part；
The Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：In parts by weight include with
Lower raw material is prepared：It is prepared in parts by weight including following raw material：50 parts of frankincense, 35 parts of caulis piperis futokadsurae, kelp 35
Part, 33 parts of the Radix Astragali, 42 parts of the fleece-flower root, 23 parts of Chinese cassia tree, 25 parts of aloe, 32 parts of hawthorn, 20 parts of Semen Cuscutae, 11 parts of caraway seed, dark plum
12 parts, 12 parts of jujube, windproof 8 parts, 15 parts of radix glycyrrhizae.
Embodiment 4：
A kind of method of black bottom soft-shelled turtle ecologic breeding：Comprise the following steps：
(1) soft-shelled turtle pond is built：Environment is quiet, it is pollution-free to have abundant water resources, and water power has a good transport service, intakes draining easily
Fang Jianchi；Soft-shelled turtle pond position is leeward, and side is high 2 meters on the sunny side, and medial surface builds the ceramic tile of Smooth, and one layer of goose ovum is first laid in soft-shelled turtle bottom of pond portion
The fine sand of 4 centimetres of stone and then layer overlay thickness, solarization back of the body platform is also set up in soft-shelled turtle pond, is carried out disinfection before launching young soft-shelled turtle by soft-shelled turtle pond；
The mode of the sterilization be soft-shelled turtle pond is put 4/5 water, then addition sterilization hydroful soft-shelled turtle pond, soaks 2 days, makes after draining
Rinsed 3 times with clear water；
Used thimerosal is prepared from the following materials：Liquor potassic permanganate 15g/l, shrub althea flower extract solution 25g/l,
Sophora alopecuroide extract solution 25g/l, madder extract solution 35g/l；
(2) young soft-shelled turtle aquaculture management：The throw-in density of young soft-shelled turtle is 10/m2, the feed containing Chinese herbal and crude drugs preparations is launched daily
0.3kg/ only, is divided into and launched three times for one day, and launch 30% in the morning, launches 40% afternoon, launches 30% at night, keeps water temperature 20
DEG C, water pH is 6.8；
(3) marketable turtle culture management：Soft-shelled turtle pond is put into after into soft-shelled turtle, also sets up a laying eggs room into soft-shelled turtle pond, there is any discrepancy for spawning setting
Mouthful, laid inside fine sand, the density launched into soft-shelled turtle pond is 2/m2, the feed 0.2kg/ containing Chinese herbal and crude drugs preparations is launched daily
Only, pasture and water 0.04kg/；
(4) daily management：Periodically carry out water quality cleaning, observation sterilizes Routine Management into soft-shelled turtle situation, soft-shelled turtle pond；
(5) capture：Capture a few days ago stops feeding, the draining of soft-shelled turtle pond, is chosen as soft-shelled turtle seizure, it is clean rear every that clear water is flushed into soft-shelled turtle
Only use woven bags packaging；
The feed containing Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：52 parts of rice bran,
32 parts of fish meal, 6 parts of premix, 4 parts of soya-bean oil, 4 parts of fresh and alive yellow meal worm, 0.2 part of salt, 2 parts of zeolite powder, 12 parts of Chinese herbal and crude drugs preparations；
The Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：In parts by weight include with
Lower raw material is prepared：It is prepared in parts by weight including following raw material：70 parts of frankincense, 45 parts of caulis piperis futokadsurae, kelp 40
Part, 38 parts of the Radix Astragali, 48 parts of the fleece-flower root, 27 parts of Chinese cassia tree, 30 parts of aloe, 37 parts of hawthorn, 27 parts of Semen Cuscutae, 14 parts of caraway seed, dark plum
18 parts, 18 parts of jujube, windproof 12 parts, 20 parts of radix glycyrrhizae.
Embodiment 5：
A kind of method of black bottom soft-shelled turtle ecologic breeding：Comprise the following steps：
(1) soft-shelled turtle pond is built：Environment is quiet, it is pollution-free to have abundant water resources, and water power has a good transport service, intakes draining easily
Fang Jianchi；Soft-shelled turtle pond position is leeward, and side is high 2 meters on the sunny side, and medial surface builds the ceramic tile of Smooth, and one layer of goose ovum is first laid in soft-shelled turtle bottom of pond portion
The fine sand of 3 centimetres of stone and then layer overlay thickness, solarization back of the body platform is also set up in soft-shelled turtle pond, is carried out disinfection before launching young soft-shelled turtle by soft-shelled turtle pond；
The mode of the sterilization be soft-shelled turtle pond is put 4/5 water, then addition sterilization hydroful soft-shelled turtle pond, soaks 2 days, makes after draining
Rinsed 3 times with clear water；
Used thimerosal is prepared from the following materials：Liquor potassic permanganate 18g/l, shrub althea flower extract solution 30g/l,
Sophora alopecuroide extract solution 28g/l, madder extract solution 39g/l；
(2) young soft-shelled turtle aquaculture management：The throw-in density of young soft-shelled turtle is 10/m2, the feed containing Chinese herbal and crude drugs preparations is launched daily
0.3kg/ only, is divided into and launched three times for one day, and launch 30% in the morning, launches 40% afternoon, launches 30% at night, keeps water temperature 23
DEG C, water pH is 7.1；
(3) marketable turtle culture management：Soft-shelled turtle pond is put into after into soft-shelled turtle, also sets up a laying eggs room into soft-shelled turtle pond, there is any discrepancy for spawning setting
Mouthful, laid inside fine sand, the density launched into soft-shelled turtle pond is 2/m2, the feed 0.1kg/ containing Chinese herbal and crude drugs preparations is launched daily
Only, pasture and water 0.02-0.05kg/；
(4) daily management：Periodically carry out water quality cleaning, observation sterilizes Routine Management into soft-shelled turtle situation, soft-shelled turtle pond；
(5) capture：Capture a few days ago stops feeding, the draining of soft-shelled turtle pond, is chosen as soft-shelled turtle seizure, it is clean rear every that clear water is flushed into soft-shelled turtle
Only use woven bags packaging；
The feed containing Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：58 parts of rice bran,
38 parts of fish meal, 9 parts of premix, 4.5 parts of soya-bean oil, 4.5 parts of fresh and alive yellow meal worm, 0.4 part of salt, 4.5 parts of zeolite powder, Chinese herbal medicine system
14 parts of agent；
The Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：In parts by weight include with
Lower raw material is prepared：It is prepared in parts by weight including following raw material：60 parts of frankincense, 40 parts of caulis piperis futokadsurae, kelp 37
Part, 35 parts of the Radix Astragali, 45 parts of the fleece-flower root, 25 parts of Chinese cassia tree, 28 parts of aloe, 35 parts of hawthorn, 25 parts of Semen Cuscutae, 12 parts of caraway seed, dark plum
14 parts, 15 parts of jujube, windproof 10 parts, 18 parts of radix glycyrrhizae.
1.1 test sites, condition：
Selection health, the basically identical childhood ink bottom soft-shelled turtle 600 of quality, mass of foundation is 265.5 ± 0.08g, at random
It is divided into 6 groups, respectively feeding embodiment 1-6 (embodiment 6 is not added with the feed containing Chinese herbal and crude drugs preparations for control) feed, even
Continue to 5 black bottom soft-shelled turtles are randomly choosed after black bottom soft-shelled turtle feeding 15 days, carry out venous blood collection, 3500r/min is centrifuged 6 minutes, takes serum
It is standby in 5 ml centrifuge tubes；Lysozyme (LSZ), catalase (CAT), superoxide dismutase (SOD), Complement C_3 are lived
Property, albumin content, CER content, total protein content using by Nanjing build up biotechnology research institute production examination
The assay method measure of agent box.
Table 1
| LSZ(U/ml) | CAT(U/ml) | SOD(U/ml) | Complement C_3 (U/ml) | |
| Embodiment 1 | 0.038±0.015a | 2.86±0.06a | 89.56±0.14a | 0.3561±0.008a |
| Embodiment 2 | 0.042±0.020b | 2.94±0.06b | 92.16±0.15b | 0.3644±0.007b |
| Embodiment 3 | 0.041±0.014b | 2.90±0.06b | 90.77±0.14b | 0.3575±0.005a |
| Embodiment 4 | 0.043±0.021b | 2.96±0.06b | 93.52±0.17b | 0.3609±0.006b |
| Embodiment 5 | 0.048±0.018b | 2.99±0.06b | 96.44±0.15b | 0.3692±0.008b |
| Embodiment 6 | 0.014±0.011c | 1.01±0.07c | 40.26±0.16c | 0.2013±0.005c |
Remarks：The different English alphabets that indicate in table after same row represent there is significant difference (P ＜ 0.05).
Lysozyme (LSZ) of the additive of Chinese herbal medicine used in this application to black bottom soft-shelled turtle, peroxidating as can be seen from the table
Hydrogen enzyme (CAT), superoxide dismutase (SOD), Complement C_3 increase significantly, and from low dose to being stepped up to big
Lysozyme (LSZ), catalase (CAT), superoxide dismutase (SOD), the growth trend of Complement C_3 are equal when dosage
Dropped afterwards first to increase, the amount for illustrating to add Chinese medicinal herb medicament is also required to reasonable control, in lysozyme compared with blank control
(LSZ), there is significant difference in catalase (CAT), superoxide dismutase (SOD), Complement C_3.
Detection of the table 2 to blood physiological and biochemical indices
| Albumin content (mg/l) | CER content (U/l) | Total protein content (g/l) | |
| Embodiment 1 | 23.34±1.20a | 43.15±1.01a | 81.12±1.10a |
| Embodiment 2 | 26.20±1.24a | 47.13±1.02a | 82.62±1.10a |
| Embodiment 3 | 30.35±1.23a | 50.31±1.01a | 87.21±1.11a |
| Embodiment 4 | 28.18±1.21a | 49.21±1.02a | 85.34±1.12a |
| Embodiment 5 | 32.13±1.22a | 50.01±1.00b | 89.26±1.12a |
| Embodiment 6 | 14.14±1.20b | 30.15±1.21c | 42.12±1.01b |
Remarks：The different English alphabets that indicate in table after same row represent there is significant difference (P ＜ 0.05).
The additive of Chinese herbal medicine used in this application is to albumin content, CER content, total as can be seen from the table
Protein content is to be compared from embodiment 1- embodiments 5, albumin content, CER content, total protein content from
Low dosage first increases to high dose to drop afterwards, embodiment 5 dosage to albumin content, CER content, gross protein
The different work of most significant difference compared with blank control of content.
According to embodiment 1-6 breeding way, after feeding 3 months, embodiment 1-5 survival rate reaches 90 ± 6%, implements
The survival rate of example 6 is 82%.
Soft-shelled turtle pond after the application sterilizes liquid disinfectant carries out inwall detection, and discovery is disappeared compared to only using potassium permanganate
The pernicious bacteria number that soft-shelled turtle pond inwall after poison contains is not only high, and flora species is more.
To embodiment 1-6 into each 3 mu of cultivation after soft-shelled turtle, the black bottom soft-shelled turtle that band captures on the same day carries out output value record, by capture
Black bottom soft-shelled turtle carries out entirety and weighed, and repetition is weighed 3 times, and yield see the table below：
| Yield (kg/ mus) | |
| Embodiment 1 | 1572±6.3 |
| Embodiment 2 | 1810±5.4 |
| Embodiment 3 | 1652±6.3 |
| Embodiment 4 | 1731±4.2 |
| Embodiment 5 | 1547±5.4 |
| Embodiment 6 | 1022±3.8 |
As seen from the above table, the black bottom soft-shelled turtle yield of the present processes cultivation is higher, and economic benefit is more preferable.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore limitation of the scope of the invention can not be interpreted as.It should be pointed out that for the person of ordinary skill of the art,
Without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection model of the present invention
Enclose.Therefore, protection scope of the present invention should be determined by the appended claims.
Claims (8)
- A kind of 1. method of black bottom soft-shelled turtle ecologic breeding：It is characterized in that：Comprise the following steps：(1) soft-shelled turtle pond is built：Environment is quiet, it is pollution-free to have abundant water resources, and water power is had a good transport service, the convenient place of draining of intaking is built Pond；Soft-shelled turtle pond position is leeward, and side is high 2 meters on the sunny side, and medial surface builds the ceramic tile of Smooth, and it is right that one layer of cobblestone is first laid in soft-shelled turtle bottom of pond portion The fine sand of 2-5 centimetres of layer overlay thickness afterwards, solarization back of the body platform is also set up in soft-shelled turtle pond, is carried out disinfection before launching young soft-shelled turtle by soft-shelled turtle pond；(2) young soft-shelled turtle aquaculture management：The throw-in density of young soft-shelled turtle is 10/m2, the feed 0.2- containing Chinese herbal and crude drugs preparations is launched daily 0.3kg/, it is divided within one day and launches three times, launch 30% in the morning, launches 40% afternoon, at night dispensing 30%, holding water temperature 22 ± 2 DEG C, water pH is 7.0 ± 0.2；(3) marketable turtle culture management：Soft-shelled turtle pond is put into after into soft-shelled turtle, also sets up a laying eggs room into soft-shelled turtle pond, spawning is provided with gateway, interior Fine sand is laid in portion, and the density launched into soft-shelled turtle pond is 2/m2, the feed 0.1-0.2kg/ containing Chinese herbal and crude drugs preparations is launched daily Only, pasture and water 0.02-0.05kg/；(4) daily management：Periodically carry out water quality cleaning, observation sterilizes Routine Management into soft-shelled turtle situation, soft-shelled turtle pond；(5) capture：Capture a few days ago stops feeding, the draining of soft-shelled turtle pond, is chosen as soft-shelled turtle seizure, clear water, which is flushed into clean latter every of soft-shelled turtle, to be made With woven bags packaging；The feed containing Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：Rice bran 50-60 parts, fish Powder 30-40 parts, premix 5-10 parts, soya-bean oil 3-5 parts, fresh and alive yellow meal worm 3-5 parts, salt 0.1-0.5 parts, zeolite powder 1-5 parts, in Herbal medicinal product 10-15 parts；The Chinese herbal and crude drugs preparations is prepared including following raw material in parts by weight：40 parts -80 parts of frankincense, caulis piperis futokadsurae 30 - 50 parts of part, 30 parts -50 parts of kelp, 30 parts -40 parts of the Radix Astragali, 40 parts -50 parts of the fleece-flower root, 20 parts -30 parts of Chinese cassia tree, 20 part -35 of aloe Part, 30 parts -40 parts of hawthorn, 15 parts -30 parts of Semen Cuscutae, 10 parts -15 parts of caraway seed, 10 parts -20 parts of dark plum, 10 parts -20 parts of jujube, Windproof 5 parts -15 parts, 10 parts -25 parts of radix glycyrrhizae.
- 2. the method for black bottom soft-shelled turtle ecologic breeding according to claim 1, it is characterised in that：Chinese herbal and crude drugs preparations is according to parts by weight Number meter includes following raw material and is prepared：50 parts -70 parts of frankincense, 35 parts -45 parts of caulis piperis futokadsurae, 35 parts -40 parts of kelp, the Radix Astragali 33 Parts -38 parts, 42 parts -48 parts of the fleece-flower root, 23 parts -27 parts of Chinese cassia tree, 25 parts -30 parts of aloe, 32 parts -37 parts of hawthorn, 20 parts of Semen Cuscutae - 27 parts, 11 parts -14 parts of caraway seed, 12 parts -18 parts of dark plum, 12 parts -18 parts of jujube, windproof 8 parts -12 parts, 15 parts -20 parts of radix glycyrrhizae.
- 3. the method for black bottom soft-shelled turtle ecologic breeding according to claim 1, it is characterised in that：Chinese herbal and crude drugs preparations is according to parts by weight Number meter includes following raw material and is prepared：60 parts of frankincense, 40 parts of caulis piperis futokadsurae, 37 parts of kelp, 35 parts of the Radix Astragali, 45 parts of the fleece-flower root, meat It is 25 parts of osmanthus, 28 parts of aloe, 35 parts of hawthorn, 25 parts of Semen Cuscutae, 12 parts of caraway seed, 14 parts of dark plum, 15 parts of jujube, windproof 10 parts, sweet 18 parts of grass.
- 4. the method for black bottom soft-shelled turtle ecologic breeding according to claim 1, it is characterised in that：The mode of the sterilization is by soft-shelled turtle 4/5 water is put in pond, and then addition sterilization hydroful soft-shelled turtle pond, soaks 1-2 days, is rinsed 3 times using clear water after draining.
- 5. the method for black bottom soft-shelled turtle ecologic breeding according to claim 1, it is characterised in that：Thimerosal used in sterilization by Following raw material is prepared：Liquor potassic permanganate 10-20g/l, shrub althea flower extract solution 15-35g/l, Sophora alopecuroide extract solution 20- 30g/l, madder extract solution 30-40g/l.
- 6. the method for black bottom soft-shelled turtle ecologic breeding according to claim 5, it is characterised in that：The shrub althea flower extract solution be by Dry after adding the alcohol solution dipping 5-10 minutes that mass concentration is 75% after shrub althea flower addition diatomite is pulverized, pressing Cohobation 10-15 minutes are carried out under the conditions of power is 0.1MPa, temperature is 35 DEG C, it is the rose of Sharon then to carry out press filtration and obtain filtrate Flower extract solution.
- 7. the method for black bottom soft-shelled turtle ecologic breeding according to claim 5, it is characterised in that：The Sophora alopecuroide extract solution be by Fresh Sophora alopecuroide adds 5 times of volume of water after smashing to pieces and carries out surname extraction, obtains filtrate, adds the ethanol that mass concentration is 75% Centrifuged after solution alcohol extracting, take filtrate to can obtain Sophora alopecuroide extract solution.
- 8. the method for black bottom soft-shelled turtle ecologic breeding according to claim 5, it is characterised in that：The madder extract solution is will be complete Grass adds 3 times of volume of water and carries out filtering to take filtrate after decocting 30 minutes, is then concentrated into 1.21 times of original volume, it is dense to add quality Spend after the ethanol solution alcohol extracting for 85% and centrifuge, take filtrate to can obtain madder extract solution.
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Cited By (2)
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| CN107648432A (en) * | 2017-10-31 | 2018-02-02 | 广西平南县平原农牧有限公司 | A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity |
| CN112273327A (en) * | 2020-11-12 | 2021-01-29 | 湖南省河洲生态甲鱼养殖有限公司 | Bionic soft-shelled turtle breeding method |
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Cited By (2)
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| CN107648432A (en) * | 2017-10-31 | 2018-02-02 | 广西平南县平原农牧有限公司 | A kind of Chinese medicine composition for improving black bottom soft-shelled turtle immunity |
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Application publication date: 20180116 |