CN107574117A - A kind of preparation facilities and its method of penicillin resistant class amonifying bacteria microbial inoculum - Google Patents
A kind of preparation facilities and its method of penicillin resistant class amonifying bacteria microbial inoculum Download PDFInfo
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Abstract
The invention belongs to the technical field using microorganism to waste water control, it is related to a kind of preparation facilities and its method of penicillin resistant class amonifying bacteria microbial inoculum, the device of the present invention, including water tank inlet, A/O reactors, second pond, the A/O reactors are divided into four rooms of field word length cube shape be arrangeding in parallel, and cell partitions are provided between each room；Agitator is provided with anoxic compartment, first aerobic compartment, the second aerobic compartment, the 3rd aerobic compartment are respectively equipped with sand head aeration head and dissolved oxygen meter, using apparatus of the present invention and method, the organic nitrogen in waste water produced from penicillin production, sanitary sewage can be effectively removed.Microbial inoculum rapid development simultaneously, obvious processing effect is adaptable, energy fast enriching, beneficial to the application in actual motion.And microbial inoculum oxygen demand during production application is low, aeration quantity is reduced, operating cost is saved, organic nitrogen removal efficiency high, there is great environmental benefit and economic benefit.
Description
Technical field
The present invention relates to a kind of preparation facilities and its method of penicillin resistant class amonifying bacteria microbial inoculum, it is applied in penicillin
The application of denitrogenation processing in class pharmacy waste water and municipal sewage, belong to the technical field to waste water control using microorganism.
Background technology
China is penicillin medicine big producer, and waste water produced from penicillin production, which is that the high concentration of a kind of difficult for biological degradation is nitrogenous, to be had
Machine waste water, the principal mode of nitrogen has organic nitrogen and ammoniacal nitrogen in waste water, and typically based on organic nitrogen, it mainly includes albumen
Matter, polypeptide, amino acid nucleic acids etc..Biological denitrificaion mainly includes three ammonification, nitrification and denitrification processes, and wherein ammonifying process is
Amonifying bacteria is converted into organic nitrogen the process of ammonia nitrogen, is the beginning of whole denitrification process, directly affects the denitrification effect in later stage；
Nitrifying process is by NH first by ammonia oxidation bacteria4 +- N is oxidized to NO2 -- N's, followed by by NOB by NO2 -- N oxygen
Turn to NO3 -- N nitrite nitrogen oxidation stage；Denitrification process is by caused NO3 -- N is under scarce/anaerobic condition by anti-
Nitrobacteria is converted into nitrogenous gas (nitrous oxide or nitrogen).Traditional biological denitrification process mainly includes nitrification and denitrification
Two stages, but containing macromolecular nitrogen substances such as a large amount of protein in pharmacy waste water, these materials are in traditional denitrification process
Difficulty is removed, water outlet total nitrogen concentration is higher；Organic nitrogen removal is inefficient simultaneously and sewage effluents total nitrogen is difficult to further
The principal element of reduction, these water outlets cause nutrients in rivers and lakes to increase, eutrophication occur.There is researcher to carry
Go out, organic nitrogen is controlled in below 3mg/L, can effectively control body eutrophication.Late nineteen nineties in last century, in environment
Antibiotic cause most attention in the world, as a result show antibiotic generally existing in different environments, including surface water, life
Sewage, underground water and drinking water, the presence of these antibiotic reduce the efficiency of denitrogenation flora, are surface water body eutrophication
One of major reason.
There is the research work in terms of the culture and microbial inoculum development of many document reports nitrification and denitrification bacterium.Such as：
Chinese invention patent (application number：200910058558.4) disclose a kind of autotrophy heterotrophism symbiosis ammonia oxidation for handling ammonia nitrogen waste water
Microbial inoculum and application thereof, the microbial inoculum are formed by mixed culture such as nitrosomonas, hot monad, pseudomonad, achromobacters；In
State's patent of invention (application number：200610020315.8) disclose a kind of preparation side for handling the microbial bacterial agent of ammonia nitrogen in waste water
Method and application thereof, the microbial inoculum are made up of 3 plants of heterotrophic ammonia-oxidizing bacterium；Chinese invention patent (application number：200610128300.3) public
Opened it is a kind of containing nitrobacteria, denitrifying bacteria, photosynthetic bacteria, polyP bacteria, bacillus, saccharomycete microorganism complex,
And the method using the complexing agent Treating Municipal Sewage, but the research of amonifying bacteria microbial inoculum is less.This microbial inoculum has efficient resistance to green grass or young crops
Mycin ammoniation and the decomposition efficiency that organic nitrogen can be significantly improved, while there is nitrification and denitrification performance.The microbial inoculum is used
In strengthening wastewater biological denitrificaion, accelerate the decomposition of algae in earth's surface eutrophication water, it is significant to reduce nitrate pollution.
The content of the invention
The present invention is the deficiency that solves the above problems, there is provided a kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum and
Its method.The amonifying bacteria microbial inoculum can be solved under the conditions of containing penicillin in waste water, and traditional biological denitrogenation method is to machine nitrogen ammonification
The problem of inefficient, the microbial inoculum can greatly improve startup time and the treatment effeciency of nitrogenous effluent biological treatment.
The present invention is achieved using following technical scheme：
A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum of the present invention, including water tank inlet, A/O reactors, two
Heavy pond, the A/O reactors are divided into four rooms of field word length cube shape be arrangeding in parallel, cell partitions are provided between each room, described
Four rooms are respectively anoxic compartment, the first aerobic compartment, the second aerobic compartment, the 3rd aerobic compartment；It is provided with and stirs in anoxic compartment
Device is mixed, mixing arm area is 18cm2, the first aerobic compartment, the second aerobic compartment, sand is respectively equipped with the 3rd aerobic compartment
Head aeration head, the first aerobic compartment, the second aerobic compartment, is respectively equipped with dissolved oxygen meter in the 3rd aerobic compartment；Sand head exposes
The internal diameter of gas head is 4cm.
The A/O reactors total measurement (volume) is 20L, and dischargeable capacity 16L, A/O reactor is divided into 4 compartments, and each compartment has
Effect volume is 4L.
Anoxic compartment, the first aerobic compartment AB cell partitions and A/O reactor walls between tip position be provided with the length of side
For the AB cell partitions square upper ports of reactor wall length 1/10th, the top of AB cell partitions square upper ports goes out
Mouth is apart from top 10mm.
The first aerobic compartment, the second aerobic compartment BC cell partitions and A/O reactor walls between tip position be provided with
The length of side is the BC cell partitions square lower ports of reactor wall length 1/10th, under BC cell partitions square lower ports
Portion is exported apart from bottom 10mm.
The second aerobic compartment, the 3rd aerobic compartment CD cell partitions and A/O reactor walls between tip position be provided with
The length of side be reactor wall length 1/10th CD cell partitions square upper ports, CD cell partitions square upper ports it is upper
Portion outlet is located at top 10mm.
The water tank inlet top is provided with water inlet switch, and bottom is provided with effluent switch, and water tank inlet passes through effluent switch, entered
The water inlet switch of the anoxic compartment of water pump, water inlet pipe and A/O reactors is sequentially connected, and anoxic compartment side walls are additionally provided with return duct and opened
Close, carry out sludge reflux, be provided with agitator in anoxic compartment, the first aerobic compartment, the second aerobic compartment, in the 3rd aerobic compartment
It is respectively equipped with sand head aeration head, the first aerobic compartment, the second aerobic compartment, is respectively equipped with DO determination in the 3rd aerobic compartment
Instrument, the first aerobic compartment and the 3rd aerobic compartment are sequentially connected gas flowmeter and air pump by pipeline respectively；Anoxic compartment sides
The return duct switch of wall is connected by pipeline through the bottom of sludge reflux pump and second pond, and leads to sludge from final clarifier mouth；It is described
The outer wall of 3rd aerobic compartment is connected with A/O reactor outlet pipes, is connected through pipeline with the middle part of second pond side, second pond
Another upper lateral part is provided with secondary clarifier effluent pipe, the excess sludge of sludge from final clarifier mouth discharge, the penicillin resistant as produced in batches
Class amonifying bacteria microbial inoculum.
The described device for preparing penicillin resistant class amonifying bacteria microbial inoculum, the second pond be top be two sections of external diameters not
Same, internal diameter identical cylinder, bottom is cone, and sludge from final clarifier mouth is located at the tapering bottom of cone.
The described device for preparing penicillin resistant class amonifying bacteria microbial inoculum, the A/O reactors are lucite material.
The method that penicillin resistant class amonifying bacteria microbial inoculum is prepared using said apparatus, is comprised the following steps：
A. amonifying bacteria enriched medium is prepared：Each material composition of amonifying bacteria enriched medium is：Peptone 5g, NaCl
0.25g, FeSO4·7H2O 0.01g, K2HPO40.5g, MgSO4·7H2O 0.5g, trace element solution 2ml, ultra-pure water 1L,
pH 7.2；Each material is sequentially placed into triangular flask, triangular flask is put into high-pressure sterilizing pot, sterilize 30min at 121 DEG C, obtains
It is standby to amonifying bacteria enriched medium；
The micro- solution mass, which forms, is：EDTA 0.4g, ZnSO4·7H2O 0.22g, CoCl6H2O
0.25g, MnCl20.25g, CuSO4·5H2O 0.1g, Na2MoO4·2H2O 0.15g, H3BO30.15g, ultra-pure water 1L；
B. the domestication culture of amonifying bacteria:Domestication culture for the first time, step a 100mL is added in 250mL shaking flask one
The amonifying bacteria enriched medium of sterilizing, then measure 50mL activated sludge (North China pharmacy waste water produced from penicillin production A/O handling process
Second pond activated sludge), it is added in shaking flask one, adds 0.01g penicillin, it is primary domestication liquid to obtain mixed liquor；At 25 DEG C,
Cultivate 24h under 150r/min oscillating conditions, shaking flask preferably oxygen condition, obtain first time sludge acclimatization bacterium solution；Second of domestication training
Support, take step a amonifying bacteria enriched medium 100mL to be fitted into shaking flask two again, take 50mL first time sludge acclimatization bacterium solutions
Supernatant be transferred in shaking flask two, cultivate 24h under 25 DEG C, 150r/min oscillating conditions, the preferably oxygen condition of shaking flask two, obtain
Second of sludge acclimatization bacterium solution；Third time domestication culture, take step a amonifying bacteria enriched medium 100mL to load again and shake
In bottle three, the supernatant of 50mL first time sludge acclimatization bacterium solutions is taken to be transferred in shaking flask three, in 25 DEG C, 150r/min oscillating conditions
Lower culture 24h, the preferably oxygen condition of shaking flask three, obtains third time sludge acclimatization bacterium solution；Domestication culture repeats five times, obtains the 5th time
Sludge acclimatization bacterium solution, as amonifying bacteria sludge acclimatization bacterium solution, its mixed liquor volatile suspended solid, MLVSS concentration (MLVSS) concentration are
4000mg/L。
C, microbial inoculum is prepared by A/O reactors；In A/O reactors four compartments add 200mL the 5th time ammonification it is thin
Bacterium sludge acclimatization bacterium solution, water inlet switch is opened, sewage enters anoxic compartment, and agitator is stirred, and sewage sequentially enters first
Aerobic compartment, the second aerobic compartment, the 3rd aerobic compartment, while the return duct switch of anoxic compartment side walls is opened, carry out sludge
Backflow, 75%, sewage passes sequentially through AB cell partitions squares upper port, BC cell partitions in the reactor for reflux ratio control
Square lower port, CD cell partitions square upper ports flow to the 3rd aerobic compartment room, and treated liquid is reacted by A/O
Device outlet pipe is discharged into second pond, and supernatant is discharged by secondary clarifier effluent pipe, and excess sludge is discharged by sludge from final clarifier mouth, from
And it is penicillin resistant class amonifying bacteria microbial inoculum that penicillin resistant class amonifying bacteria microbial inoculum, which is made,.
Preferably, the described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, in the anoxic compartment area of A/O reactors
Interior, stirring vane rotating speed is 200r/min, and dissolved oxygen concentration is less than 0.2mg/L.
Preferably, the described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is the first aerobic compartment, second best
Oxygen compartment, the 3rd aerobic compartment Dissolved Oxygen concentration Control are in 0.5mg/L, and pH controls are 7.0~8.0, water-carrying capacity 80L/d, water
The power residence time is 4.8h.
Preferably, the described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is the first aerobic compartment, second best
Oxygen compartment, the reflux ratio of the 3rd aerobic compartment water-carrying capacity are 75%.
Preferably, the described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, the 3rd aerobic compartment discharge
Excess sludge, the i.e. MLVSS of penicillin resistant class amonifying bacteria microbial inoculum are 13000mg/L.
Preferably, the described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, first is aerobic in the A/O reactors
Compartment, the second aerobic compartment, the MLVSS of the 3rd aerobic compartment are 4500mg/L.
The described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, the residue of the 3rd aerobic compartment discharge are dirty
Mud, the i.e. extracted DNA sequencing of penicillin resistant class amonifying bacteria microbial inoculum, analysis show that the penicillin resistant class amonifying bacteria microbial inoculum includes
Acinetobacter calcoaceticus (Acinetobacter sp.A8), bacillus (Bacillus sp.), Staphylococcus pasteuri
(Staphylococcus pasteuri strain), microbacterium (Exiguobacterium), comamonas (Comamonas
Sp.), Aeromonas media (Aeromonas media strain), Chryseobacterium sp (Chryseobacterium sp), short steady
Bacillus (Empedobacter. (resistance)), serratia marcescens (Serratia marcescens strain), conciliate keratan
Microbacterium (Microbacterium keratanolyticum), hay bacillus (Bacillussubtilis sp.), soil bar
Bacteria strain (Agrobacterium sp.).
The remarkable advantage that has compared with prior art of the present invention is：
(1) amonifying bacteria microbial inoculum Ammoniation efficiency in the waste water of the 10mgL-1 containing penicillin is very high.Highest ammonification speed
Rate reaches as high as 30.5mg organic nitrogens/(g SSh) in 24h, and organic nitrogen degradation effect is excellent, far beyond being reported in other documents
The ammonification effect of road microbial inoculum.
(2) the amonifying bacteria microbial inoculum auxotype is heterotroph, grows rapidly, can quickly increase under the conditions of containing penicillin
Grow, it is possible to reduce initial cost and dosage.
(3) the amonifying bacteria microbial inoculum low manufacture cost, small investment, beneficial to a large amount of productions.
(4) present invention is applicable not only to the processing of the waste water containing penicillins, it may also be used for other class antibiotic waste waters, city
The improvement of dirty (useless) water containing organic nitrogen such as sanitary sewage, enriched surface water, has a extensive future, and has higher society's effect
Benefit.
Brief description of the drawings
Fig. 1, apparatus of the present invention structural representation.
Fig. 2 is the schematic diagram of four cell partitions of A/O reactors in apparatus of the present invention.
Fig. 3 is the organic nitrogen removal efficiency chart of the inventive method.
Embodiment
The embodiment of the present invention is described in detail with reference to the accompanying drawings.
Referring to Fig. 1 to Fig. 3.
Each part numbers are in figure：
1. the water inlet switch 7. of 5. water inlet pipe of water inlet switch 2. water tank inlet, 3. effluent switch, 4. intake pump 6. flows back
Pipe switch 8.A/O reactor 10. air pumps of 9.A/O reactors outlet pipe 11. sand head aeration head, 12. agitators 13. 2 are heavy
The secondary clarifier effluent pipe 19 2 of pond 14. gas flowmeter, 15. dissolved oxygen meter, 16 sludge reflux pump, 17. return duct 18 is heavy
Pond mud discharging mouth.
Anoxic compartment a, the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d；
On AB cell partitions square upper port a1, BC cell partitions square lower port b1, CD cell partitions squares
Port c1.
A kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum, including water tank inlet 2, A/O reactors 8, second pond
13, four rooms of field word length cube shape that the A/O reactors 8 divide to be arranged in parallel are provided with cell partitions between each room, and described four
Individual room is respectively anoxic compartment a, the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d；It is provided with anoxic compartment a
Agitator 12, mixing arm area A=18cm2, interior point of the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d
Not She You sand head aeration head 11, the first aerobic compartment b, the second aerobic compartment c, be respectively equipped with dissolved oxygen in the 3rd aerobic compartment d
Analyzer (15)；The internal diameter of sand head aeration head 11 is 4cm.
The total measurement (volume) of A/O reactors 8 is 20L, and dischargeable capacity 16L, A/O reactor is divided into 4 compartments, and each compartment has
Effect volume is 4L.
Anoxic compartment a, the first aerobic compartment b AB cell partitions and A/O reactor walls between tip position be provided with side
AB cell partitions square upper port a1, the AB cell partitions square upper ports a1's of a length of reactor wall length 1/10th
Upper outlet is apart from top 10mm.
The first aerobic compartment b, the second aerobic compartment c BC cell partitions and A/O reactor walls between tip position set
There are BC cell partitions square lower port b1, BC the cell partitions square lower ports that the length of side is reactor wall length 1/10th
B1 lower part outlet is apart from bottom 10mm.
Aerobic compartment c, aerobic compartment d CD cell partitions and A/O reactor walls between tip position be provided with the length of side
CD cell partitions square upper port c1, the CD cell partitions square upper ports c1 of reactor wall length 1/10th top
Outlet is located at top 10mm.
The top of water tank inlet 2 is provided with water inlet switch 1, and bottom is provided with effluent switch 3, and water tank inlet 2 is opened by water outlet
Close 3, intake pump 4, the anoxic compartment a water inlet switch 6 of water inlet pipe 5 and A/O reactors 8 is sequentially connected, anoxic compartment a side walls are also
Provided with return duct switch 7, carry out sludge reflux, be provided with agitator 12 in anoxic compartment a, the first aerobic compartment b, second it is aerobic every
It is respectively equipped with sand head aeration head 11 in room c, the 3rd aerobic compartment d, it is the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic
Dissolved oxygen meter 15 is respectively equipped with compartment d, and (Fig. 1 is structural representation, does not all show the first aerobic compartment b, second
Have sand head aeration head 11 and dissolved oxygen meter 15 in the aerobic aerobic compartment d of compartment c and the 3rd), the first aerobic compartment b and
Three aerobic compartment d are sequentially connected gas flowmeter 14 and air pump 10 by pipeline respectively；The return duct switch of anoxic compartment a side walls
7 are connected by pipeline through sludge reflux pump 16 with the bottom of second pond 13, and lead to sludge from final clarifier mouth 19；Described 3rd is aerobic
Compartment d outer wall is connected with A/O reactors outlet pipe 9, is connected through pipeline with the middle part of the side of second pond 13, second pond 13 it is another
One upper lateral part is provided with secondary clarifier effluent pipe 18, the excess sludge that sludge from final clarifier mouth 19 is discharged, the resistance to mould as produced in batches
Plain class amonifying bacteria microbial inoculum.
The described device for preparing penicillin resistant class amonifying bacteria microbial inoculum, the second pond 13 be top be two sections of external diameters not
Same, internal diameter identical cylinder, bottom is cone, and sludge from final clarifier mouth 19 is located at the tapering bottom of cone.
The described device for preparing penicillin resistant class amonifying bacteria microbial inoculum, the A/O reactors 8 are lucite material.
The method that described device prepares penicillin resistant class amonifying bacteria microbial inoculum, comprises the following steps：
A. amonifying bacteria enriched medium is prepared：Each material composition of amonifying bacteria enriched medium is：Peptone 5g, NaCl
0.25g, FeSO4·7H2O 0.01g, K2HPO40.5g, MgSO4·7H2O 0.5g, trace element solution 2ml, ultra-pure water 1L,
pH 7.2；Each material is sequentially placed into triangular flask, triangular flask is put into high-pressure sterilizing pot, sterilize 30min at 121 DEG C, obtains
It is standby to amonifying bacteria enriched medium；
The micro- solution mass, which forms, is：EDTA 0.4g, ZnSO4·7H2O 0.22g, CoCl6H2O
0.25g, MnCl20.25g, CuSO4·5H2O 0.1g, Na2MoO4·2H2O 0.15g, H3BO30.15g, ultra-pure water 1L；
B. the domestication culture of amonifying bacteria:Domestication culture for the first time, step a 100mL is added in 250mL shaking flask one
The amonifying bacteria enriched medium of sterilizing, then measure 50mL activated sludge (North China pharmacy waste water produced from penicillin production A/O handling process
Second pond activated sludge), it is added in shaking flask one, adds 0.01g penicillin, it is primary domestication liquid to obtain mixed liquor；At 25 DEG C,
Cultivate 24h under 150r/min oscillating conditions, shaking flask preferably oxygen condition, obtain first time sludge acclimatization bacterium solution；Second of domestication training
Support, take step a amonifying bacteria enriched medium 100mL to be fitted into shaking flask two again, take 50mL first time sludge acclimatization bacterium solutions
Supernatant be transferred in shaking flask two, cultivate 24h under 25 DEG C, 150r/min oscillating conditions, the preferably oxygen condition of shaking flask two, obtain
Second of sludge acclimatization bacterium solution；Third time domestication culture, take step a amonifying bacteria enriched medium 100mL to load again and shake
In bottle three, the supernatant of 50mL first time sludge acclimatization bacterium solutions is taken to be transferred in shaking flask three, in 25 DEG C, 150r/min oscillating conditions
Lower culture 24h, the preferably oxygen condition of shaking flask three, obtains third time sludge acclimatization bacterium solution；Domestication culture repeats five times, obtains the 5th time
Sludge acclimatization bacterium solution, as amonifying bacteria sludge acclimatization bacterium solution, its mixed liquor volatile suspended solid, MLVSS concentration (MLVSS) concentration are
4000mg/L。
C, microbial inoculum is prepared by A/O reactors 8；Four compartments add 200mL the 5th time ammonification in A/O reactors 8
Bacteria sludge tames bacterium solution, opens water inlet switch 6, and sewage enters anoxic compartment a, and agitator 12 is stirred, and sewage enters successively
Enter the one the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d, while open the return duct of anoxic compartment a side walls
Switch 7, sludge reflux is carried out, 75%, sewage passes sequentially through on AB cell partitions squares in the reactor for reflux ratio control
Port a1, BC cell partitions square lower port b1, CD cell partitions square upper port c1 flows to the 3rd aerobic compartment d rooms,
(position of a1, b1, c1 port is different, prevents from forming short stream；) treated liquid is discharged into two by A/O reactors outlet pipe 9
Heavy pond 13, supernatant are discharged by secondary clarifier effluent pipe 18, and excess sludge is discharged by sludge from final clarifier mouth 19, resistance to so as to be made
Penicillins amonifying bacteria microbial inoculum is penicillin resistant class amonifying bacteria microbial inoculum.
The described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, in the anoxic compartment a areas of A/O reactors 8, stir
It is 200r/min to mix blade rotational speed, and dissolved oxygen concentration is less than 0.2mg/L.
The described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is the one the first aerobic compartment b, second aerobic
Compartment c, the 3rd aerobic compartment d Dissolved Oxygen concentration Controls are in 0.5mg/L, and pH controls are 7.0~8.0, water-carrying capacity 80L/d, water
The power residence time is 4.8h.
The described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is the one the first aerobic compartment b, second aerobic
Compartment c, the reflux ratio of the 3rd aerobic compartment d water-carrying capacity are 75%.
The described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, the residue of the 3rd aerobic compartment d discharges are dirty
Mud, the i.e. MLVSS of penicillin resistant class amonifying bacteria microbial inoculum are 13000mg/L.
The described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, in the A/O reactors the one the first it is aerobic every
Room b, the second aerobic compartment c, the 3rd aerobic compartment d MLVSS are 4500mg/L.
The described method for preparing penicillin resistant class amonifying bacteria microbial inoculum, the residue of the 3rd aerobic compartment d discharges are dirty
Mud, the i.e. extracted DNA sequencing of penicillin resistant class amonifying bacteria microbial inoculum, analysis show that the penicillin resistant class amonifying bacteria microbial inoculum includes
Acinetobacter calcoaceticus (Acinetobacter sp.A8), bacillus (Bacillus sp.), Staphylococcus pasteuri
(Staphylococcus pasteuri strain), microbacterium (Exiguobacterium), comamonas (Comamonas
Sp.), Aeromonas media (Aeromonas media strain), Chryseobacterium sp (Chryseobacterium sp), short steady
Bacillus (Empedobacter. (resistance)), serratia marcescens (Serratia marcescens strain), conciliate keratan
Microbacterium (Microbacterium keratanolyticum), hay bacillus (Bacillussubtilis sp.), soil bar
Bacteria strain (Agrobacterium sp.).
Domestication liquid containing penicillin wastewater in anoxic compartment a is sufficiently mixed with waste water, and part nitrite nitrogen is carried out in waste water
Anti-nitration reaction, to reduce the oxygen being oxidized in aerobic compartment nitrite nitrogen in nitrate nitrogen consumption of reactor.Under equal conditions
Amonifying bacteria reaction speed is better than ammonia oxidizing bacteria, because dissolved oxygen concentration is relatively low in aerobic compartment, ammonia oxidizing bacteria reaction
It is suppressed, organic nitrogen is rapidly decomposed, amonifying bacteria amount reproduction, is produced the largely activated sludge containing amonifying bacteria, is passed through
Penicillin resistant amonifying bacteria microbial inoculum is made in sludge settling concentration.
Experiment water inlet be used as raw water using the comprehensive wastewater of penicillin production by dilution, waste water COD concentration for 600~
800mg·L-1, total nitrogen concentration be 60~120mgL-1, pH value be 6.5~7.5.Sewage is with a1 → b1 → c1 in the reactor
→ d directions are flowed, and flow automatically by current pressure difference, flow direction is a → b → c → d, and waste water passes through agitator 12 in anoxic compartment
It is sufficiently mixed, under anoxic conditions, part nitrite nitrogen is removed by denitrification, reduces this part nitrite nitrogen aerobic
Compartment consumes excessive oxygen.Under aerobic conditions, ammoniation reaction speed has ammoxidation, and organic nitrogen preferentially decomposes.
Waste water flows into the first aerobic compartment b by water High Pressure Difference through AB cell partitions square upper ports a1, contains greatly in aerobic compartment
The sewage for measuring organic nitrogen starts to react in the case where amonifying bacteria acts on；Sewage is the first aerobic compartment b's under hydraulic pressuring difference effect
BC cell partitions square lower ports b1 flows into the second aerobic compartment c, does not react complete organic nitrogen in the second aerobic compartment c
Continue to react, sewage flows into the in the second aerobic compartment c CD cell partitions square upper ports c1 under hydraulic pressuring difference effect
Three aerobic compartment d, remaining organic nitrogen continues to react in the 3rd aerobic compartment d.Stirred in the process of running in anoxic compartment a
Device rotating speed, stirring vane rotating speed are 200r/min, and dissolved oxygen concentration is less than 0.2mg/L, sand head aeration head 11 in course of reaction, are adjusted
Solar term flowmeter body 14, the first aerobic compartment b, the second aerobic compartment c, the 3rd aerobic compartment d Dissolved Oxygen concentration Controls is set to exist
0.5mg/L, wastewater flow 80L/d, waste water hydraulic detention time are 4.8h, and second pond return sludge ratio is 75%, it is aerobic every
It is efficient penicillin resistant amonifying bacteria microbial inoculum after the excess sludge concentration of room d discharges, amonifying bacteria microbial inoculum MLSS is
MLSS is 4500mg/L in 13000mg/L, A/O reactor.
Technology application effect 1：
The measure of amonifying bacteria domestication liquid ammonification ability：Ammonification and the nitrification of amonifying bacteria are monitored while enrichment process
Ability, its ammonification rate is determined, monitor the other of the change of the microbial inoculum ammonification ability, Ammoniation efficiency best time and the microbial inoculum
Function.Take laboratory sample 10mL to centrifuge 10min under 6000rpm every time, take domestication liquid supernatant measure total nitrogen, ammonia nitrogen, nitrous
The content of hydrochlorate and nitrate.Ammonia nitrogen is determined using Nessler's reagent photometer, and nitrite is using diazonium-azo method measure, nitre
Hydrochlorate uses determined by ultraviolet spectrophotometry, and sludge concentration (MLSS) uses gravimetric detemination.Experimental result is as shown in table 1.Such as
Shown in Fig. 3, Fig. 3 abscissa is days running, and ordinate is that the left side is Inlet and outlet water total nitrogen, ammonia nitrogen concentration, is on the right of ordinate
Nitrogen removal rate.
The functional test of the amonifying bacteria domestication liquid of table 1
In order to promote initial quick domestication amplification, 5 times enrichment condition is identical, penicillin resistant microbial inoculum in enrichment process
Ammoniation efficiency quickly increases, and it is most fast to reach speedup during 12h, and ammonification rate has declined during 12-24h, while nitrite nitrogen nitrogen closes
Increasing into stable, nitrate nitrogen synthesis rate is gradually reduced, and the gross dry weight increase of microbial inoculum is obvious during 12h, and amplification has reached 900%,
MLSS is 2600mg/L or so.Achieve the effect of efficiently concentrating.Table 1 shows that this microbial inoculum can be very good to complete under this condition
Ammonification and sub- digestion workshop section, control is beneficial at stain disease in short distance Asia digestion phase, the addition of the microbial inoculum well for denitrogenation workshop section
Manage the progress of short distance nitration.
Technology application effect 2：Amonifying bacteria microbial inoculum is to waste water produced from penicillin production treatment effect
1.5L tubualted bottles are taken, inject 1L high concentration penicillin nitrogenous effluents, add microbial inoculum 50mL, microbial inoculum MLSS is
13000mg/L, husky head are aerated supplemental oxygen, and gas flowmeter control gas flow, dissolved oxygen meter determines molten in container
Oxygen is solved, dissolved oxygen maintains 0.5mg/L or so.PH is controlled in 7.0-8.0, samples 10mL in delivery port every 12h, liquid exists
10min is centrifuged under 6000rpm, takes the content of supernatant measure total nitrogen, ammonia nitrogen, nitrite and nitrate.Wherein, ammonia nitrogen uses
Nessler's reagent photometer determines, and for nitrite using diazonium-azo method measure, total nitrogen, nitrate use ultraviolet spectrophotometry
Measure.
The ammonification microbial inoculum application experiment result of table 2
As can be seen from Table 2, the penicillin resistant ammonification microbial inoculum works well in analogue reactor, during 12h, organic nitrogen
Ammonification rate is 26.7mg organic nitrogens/(g SSh), and the conversion ratio of organic nitrogen is 81.3%；Ammonification rate is 15.5mg during 24h
Organic nitrogen/(g SSh), the conversion ratio of organic nitrogen is 96.5%.Data above shows the microbial inoculum in the processing high ammonia nitrogen of penicillin
Organic wastewater ammonification positive effect, there is very big application value.
Technology application effect 3：Amonifying bacteria microbial inoculum is to municipal sewage treatment effect
Device 8 is answered to prepare microbial inoculum by A/O, the A/O reactor dischargeable capacity 16L A/O reactors are divided into 4 compartments, each
Compartment effective volume 4L, as shown in figure 1, each aerobic compartment adds 200mL amonifying bacteria enrichment culture liquid, anoxic zone a dissolvings
Oxygen concentration is less than 0.2mg/L, and first aerobic compartment b, c, d Dissolved Oxygen concentration Control is controlled in 7.0- in 0.5mg/L or so, pH
8.0, flow 80L/d, hydraulic detention time 4.8h, reflux ratio 75%, the excess sludge of aerobic compartment d discharges is efficient
Penicillin resistant amonifying bacteria microbial inoculum, by mud discharging mouth 19 collect the efficient amonifying bacteria microbial inoculum MLSS of penicillin resistant be
13000mg/L or so, MLSS is 4500mg/L or so in reactor.10mL is sampled in delivery port every 24h, liquid exists
10min is centrifuged under 6000rpm, takes the content of supernatant measure total nitrogen, ammonia nitrogen, nitrite and nitrate.Wherein, ammonia nitrogen uses
Nessler's reagent photometer determines, and for nitrite using diazonium-azo method measure, total nitrogen, nitrate use ultraviolet spectrophotometry
Measure.Raw water is tested to intake using municipal sewage plant.Experimental result is as shown in Figure 1.Waste water is by switch 1, into water tank
2, by effluent switch 3, by intake pump 4 through water inlet pipe 5 by water inlet switch 6, into A/O reactors 8, waste water leads in compartment a
Cross agitator 12 to stir, fully reacted with the sludge tamed, the first aerobic compartment b, second are sequentially entered under plug-flow effect
Aerobic compartment c, the 3rd aerobic compartment d forms, the first aerobic compartment b, second best is controlled through gas flowmeter 14 by air pump 10
Dissolved oxygen concentration in oxygen compartment c, the 3rd aerobic compartment d, utilize the compartment b aerobic to first, second best respectively of sand head aeration head 11
Oxygen compartment c, the 3rd aerobic compartment d are aerated, using dissolved oxygen meter 15 determine the first aerobic compartment b, second it is aerobic every
Room c, the 3rd aerobic compartment d dissolved oxygen concentration, treated water enter second pond 13, place by A/O reactors outlet pipe 9
The waste water managed is discharged through secondary clarifier effluent pipe 18, and the partial sludge of second pond 13, through return duct 17, is led to by sludge reflux pump 16
Cross backflow switch 7 and arrive A/O reactors 8, to improve reactor efficiency, collect excess sludge through mud discharging mouth 19, concentration supports resistance to green grass or young crops
The efficient amonifying bacteria microbial inoculum of mycin.
As seen from Figure 3, reactor start-up is rapid, and the machine nitrogen removal efficiency of beginning with is only 45% or so, with operation
The progress of time, clearance are continuously increased, and the clearance to organic nitrogen at the 6th day reaches 90%, and amonifying bacteria is rapid in microbial inoculum
Environment, active raising ability enhancing are adapted to, amonifying bacteria starts batch and grown.Operational effect is stable during startup, to the 16th, 18
It when organic nitrogen removal rate it is stable 94% or so, removal effect is notable, shows that microbial inoculum application value is very high, and its activated sludge is
The high penicillin resistant amonifying bacteria microbial inoculum of degradation rate.
To sum up, the penicillin resistant amonifying bacteria microbial inoculum is gradual by enrichment, domestication, culture ammonification performance under aerobic conditions
Improve, can effectively remove the organic nitrogen in waste water produced from penicillin production, sanitary sewage.Microbial inoculum rapid development simultaneously, treatment effect
Significantly, it is adaptable, energy fast enriching, beneficial to the application in actual motion.And microbial inoculum is aerobic during production application
Measure it is low, reduce aeration quantity, save operating cost, organic nitrogen removal efficiency high, there is great environmental benefit and economic benefit.
Claims (10)
1. a kind of device for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, including water tank inlet (2), A/O reactors
(8), second pond (13), the A/O reactors (8) are divided into four rooms of field word length cube shape be arrangeding in parallel, are provided between each room
Cell partitions, four rooms are respectively anoxic compartment (a), the first aerobic compartment (b), the second aerobic compartment (c), the 3rd aerobic
Compartment (d)；Agitator (12) is provided with anoxic compartment (a), mixing arm area is 18cm2；First aerobic compartment (b),
Two aerobic compartments (c), it is respectively equipped with sand head aeration head (11) in the 3rd aerobic compartment (d), it is the first aerobic compartment (b), second best
Dissolved oxygen meter (15) is respectively equipped with oxygen compartment (c), the 3rd aerobic compartment (d)；The internal diameter of sand head aeration head (11) is
4cm；
A/O reactors (8) total measurement (volume) is 20L, and dischargeable capacity 16L, A/O reactor is divided into 4 compartments, and each compartment is effective
Volume is 4L；
Anoxic compartment (a), the first aerobic compartment (b) AB cell partitions and A/O reactor walls between tip position be provided with side
The AB cell partitions square upper ports (a1) of a length of reactor wall length 1/10th, AB cell partitions square upper ports
(a1) upper outlet is apart from top 10mm；
The first aerobic compartment (b), aerobic compartment (c) BC cell partitions and A/O reactor walls between tip position be provided with side
The BC cell partitions square lower ports (b1) of a length of reactor wall length 1/10th, BC cell partitions square lower ports
(b1) lower part outlet is apart from bottom 10mm；
The second aerobic compartment (c), the 3rd aerobic compartment (d) CD cell partitions and A/O reactor walls between tip position set
There are the CD cell partitions square upper ports (c1) that the length of side is reactor wall length 1/10th, CD cell partitions squares upper end
The upper outlet of mouth (c1) is located at top 10mm；
Water tank inlet (2) top is provided with water inlet switch 1, and bottom is provided with effluent switch (3), and water tank inlet (2) passes through water outlet
Switch (3), intake pump (4), the water inlet switch (6) of water inlet pipe (5) and the anoxic compartment (a) of A/O reactors (8) are sequentially connected,
Anoxic compartment (a) side wall is additionally provided with return duct switch (7), carries out sludge reflux, agitator (12) is provided with anoxic compartment (a),
First aerobic compartment (b), the second aerobic compartment (c), it is respectively equipped with sand head aeration head (11) in the 3rd aerobic compartment (d), first
Aerobic compartment (b), the second aerobic compartment (c), dissolved oxygen meter (15) is respectively equipped with the 3rd aerobic compartment (d), first is good
Oxygen compartment (b) and the 3rd aerobic compartment (d) are sequentially connected gas flowmeter (14) and air pump (10) by pipeline respectively；Anoxic every
The return duct switch (7) of room (a) side wall is connected by pipeline through sludge reflux pump (16) with the bottom of second pond (13), and is led to
Sludge from final clarifier mouth (19)；The outer wall of the aerobic compartment (d) is connected with A/O reactors outlet pipe (9), through pipeline and second pond
(13) the middle part connection of side, another upper lateral part of second pond (13) are provided with secondary clarifier effluent pipe (18), sludge from final clarifier mouth
(19) excess sludge of discharge, the penicillin resistant class amonifying bacteria microbial inoculum as produced in batches.
2. the device as claimed in claim 1 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, the second pond
(13) it is that top is two sections of external diameter differences, internal diameter identical cylinder, bottom is cone, and sludge from final clarifier mouth (19) is located at circle
The tapering bottom of cone.
3. the device as claimed in claim 1 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, the A/O reactions
Device (8) is lucite material.
4. a kind of usage right requires the method that 1 described device prepares penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that including
Following steps：
A. amonifying bacteria enriched medium is prepared：Each material composition of amonifying bacteria enriched medium is：Peptone 5g, NaCl
0.25g, FeSO4·7H2O 0.01g, K2HPO40.5g, MgSO4·7H2O 0.5g, trace element solution 2ml, ultra-pure water 1L,
pH 7.2；Each material is sequentially placed into triangular flask, triangular flask is put into high-pressure sterilizing pot, sterilize 30min at 121 DEG C, obtains
It is standby to amonifying bacteria enriched medium；
The micro- solution mass, which forms, is：EDTA 0.4g, ZnSO4·7H2O 0.22g, CoCl6H2O 0.25g,
MnCl20.25g, CuSO4·5H2O 0.1g, Na2MoO4·2H2O 0.15g, H3BO30.15g, ultra-pure water 1L；
B. the domestication culture of amonifying bacteria:Domestication culture for the first time, step a 100mL sterilizings are added in 250mL shaking flask one
Amonifying bacteria enriched medium, then measure 50mL activated sludge, be added in shaking flask one, add 0.01g penicillin, obtain mixed
Conjunction liquid is primary domestication liquid；Cultivate 24h under 25 DEG C, 150r/min oscillating conditions, shaking flask preferably oxygen condition, obtain dirty for the first time
Mud tames bacterium solution；Second of domestication culture, takes step a amonifying bacteria enriched medium 100mL to be fitted into shaking flask two, takes again
The supernatant of 50mL first time sludge acclimatization bacterium solutions is transferred in shaking flask two, is cultivated under 25 DEG C, 150r/min oscillating conditions
24h, the preferably oxygen condition of shaking flask two, obtains second of sludge acclimatization bacterium solution；Third time domestication culture, takes step a ammonification again
Enrichment of bacteria culture medium 100mL is fitted into shaking flask three, takes the supernatant of 50mL first time sludge acclimatization bacterium solutions to be transferred to shaking flask three
In, cultivate 24h under 25 DEG C, 150r/min oscillating conditions, the preferably oxygen condition of shaking flask three, obtain third time sludge acclimatization bacterium solution；
Domestication culture repeats five times, obtains the 5th sludge acclimatization bacterium solution, as amonifying bacteria sludge acclimatization bacterium solution, the volatilization of its mixed liquor
Property suspended sediment concentration (MLVSS) concentration is 4000mg/L；
C, microbial inoculum is prepared by A/O reactors (8)；Four compartments add 200mL the 5th time ammonification in A/O reactors (8)
Bacteria sludge tames bacterium solution, opens water inlet switch (6), and sewage enters anoxic compartment (a), and agitator (12) is stirred, sewage
The first aerobic compartment (b), the second aerobic compartment (c), the 3rd aerobic compartment (d) are sequentially entered, while opens anoxic compartment a side walls
Return duct switch (7), carry out sludge reflux, reflux ratio control 75%, sewage pass sequentially through in the reactor AB compartments every
Plate square upper port (a1), BC cell partitions square lower ports (b1), CD cell partitions square upper ports (c1) flow to
3rd aerobic compartment (d), treated liquid are discharged into second pond (13) by A/O reactors outlet pipe (9), and supernatant passes through two
Sinking pool effluent pipe (18) is discharged, and excess sludge is discharged by sludge from final clarifier mouth (19), thin so as to which penicillin resistant class ammonification be made
Bacteria agent is penicillin resistant class amonifying bacteria microbial inoculum.
5. the method as claimed in claim 4 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, in A/O reactors
(8) in anoxic compartment (a) area, stirring vane rotating speed is 200r/min, and dissolved oxygen concentration is less than 0.2mg/L.
6. the method as claimed in claim 4 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, described first is aerobic
Compartment (b), aerobic compartment (c), aerobic compartment (d) Dissolved Oxygen concentration Control are in 0.5mg/L, and pH controls are 7.0~8.0, current
Measure as 80L/d, hydraulic detention time 4.8h.
7. the method as claimed in claim 4 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, described first is aerobic
Compartment (b), the second aerobic compartment (c), the reflux ratio of the 3rd aerobic compartment (d) water-carrying capacity are 75%.
8. the method for penicillin resistant class amonifying bacteria microbial inoculum is prepared as claimed in claim 4, it is characterized in that, the described 3rd is aerobic
The excess sludge of compartment (d) discharge, i.e. the MLVSS of penicillin resistant class amonifying bacteria microbial inoculum is 13000mg/L.
9. the method as claimed in claim 4 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, the A/O reactions
The first aerobic compartment (b), the second aerobic compartment (c), the MLVSS of the 3rd aerobic compartment (d) are 4500mg/L in device.
10. the method as claimed in claim 4 for preparing penicillin resistant class amonifying bacteria microbial inoculum, it is characterized in that, the described 3rd is good
The excess sludge of oxygen compartment (d) discharge, the i.e. extracted DNA sequencing of penicillin resistant class amonifying bacteria microbial inoculum, analysis draw the resistance to green grass or young crops
Mycin class amonifying bacteria microbial inoculum include acinetobacter calcoaceticus (Acinetobacter sp.A8), bacillus (Bacillus sp.), bar
Family name staphylococcus (Staphylococcus pasteuri strain), microbacterium (Exiguobacterium), comamonas
(Comamonas sp.), Aeromonas media (Aeromonas media strain), Chryseobacterium sp
(Chryseobacterium sp), Empedobacter brevis (Empedobacter. (resistance)), serratia marcescens (Serratia
Marcescens strain), conciliate keratan microbacterium (Microbacterium keratanolyticum), hay bacillus
(Bacillussubtilis sp.), agrobacterium bacterial strain (Agrobacterium sp.).
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