CN107552115A - Detection means and its method of work based on microflow control technique - Google Patents
Detection means and its method of work based on microflow control technique Download PDFInfo
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- CN107552115A CN107552115A CN201710585320.1A CN201710585320A CN107552115A CN 107552115 A CN107552115 A CN 107552115A CN 201710585320 A CN201710585320 A CN 201710585320A CN 107552115 A CN107552115 A CN 107552115A
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- disc
- magnetic force
- force piece
- stopper slot
- detection means
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention provides a kind of detection means and its method of work based on microflow control technique, the detection means includes the first disc, and first disc has multiple detection units, and detection unit includes detect tank, waste liquid tank；Second disc, second disc are arranged on the lower section of first disc；Second disc has multiple magnetic force units, and the magnetic force unit is equal with the quantity of detection unit, and the magnetic force unit includes stopper slot and the first magnetic force piece；Angle between the trend of the stopper slot and the radial direction of second disc is acute angle, and first magnetic force piece is arranged in the stopper slot；Second magnetic force piece, second magnetic force piece are fixed on the outside of the end in the center of circle for facing second disc of the stopper slot；Second magnetic force piece keeps geo-stationary with second disc.The present invention has the advantages that high sensitivity, detection time are short.
Description
Technical field
The present invention relates to micro-fluidic, detection means and its method of work more particularly to based on microflow control technique.
Background technology
Microflow control technique refers to using involved by tens of to hundreds of microns of line treatment or the system of manipulation microfluid
Science and Technology, it is an emerging friendship for being related to chemistry, fluid physics, microelectronics, new material, biology and biomedical engineering
Pitch subject.
Centrifugal microfluidic control is using micro-electromechanical technology to rely on, by the sampling of chemical analysis, pretreatment, derivation, mixing and inspection
Valve, flow duct, mixing reactor, sample-adding, separation, the part such as detection being related to during surveying etc. are integrated into the disc of CD shapes
On, the driving force using centrifugal force as liquid stream, realize the micro-fluidic system to liquid stream detection and analysis.The original controlled using centrifugal rotational speed
Reason, it can only need to add reagent for testing staff, run journey easily to control the operating process of experiment by program
Sequence, experimental result can be obtained to complete the flow entirely tested.
Chemiluminescence immune assay (chemiluminescence immunoassay, CLIA) is by with high sensitivity
Chemical luminescent detecting technology be combined with the immune response of high specific, for various antigens, haptens, antibody, hormone, enzyme
With the detection and analysis technology of medicine etc..In order to improve the coated careless mistake of antigen-antibody usually using magnetic bead as carrier, by magnetic bead
It is injected into reaction in reaction tank to be coated with, the release of magnetic bead is manipulated to realize separation by magnet.
Chemiluminescence immune assay includes two parts, i.e. immune response system and chemiluminescence analysis system.Chemistry hair
Photosystem is by the catalysis of catalyst and the oxidation of oxidant, the centre of one excitation state of formation using chemiluminescent substance
Body, launch photon when the intermediate of this excitation state returns to stable ground state, light quantity is measured using luminous signal analyzer
Suboutput.Immune response system is that luminescent substance is marked directly on antigen or antibody.With enzyme effect in luminous substrate.
The manipulation of micro-fluidic centrifugal magnetic particle, the manipulation that can solve the magnetic bead in the projects such as above-mentioned chemiluminescence are asked
Topic, be good mode for above-mentioned middle cleaning link by way of mixing and shaking, cleaning it is more thorough.
Application number CN201410669355.X patent provide a kind of centrifugal magnetic particle manipulate with detection means and
Its operation method, the mode of operation is that magnetic bead is inhaled into one and periodically moved to complete antigen-antibody knot in the solution by magnet
The steps such as conjunction, cleaning and substrate colour developing.The main deficiency of the program is：
Magnet all the time inhales magnetic bead into one, loses dispersiveness；Cleaning and the substrate reactions stage, top lower magnet
It is mobile that to drive magnetic bead movement, so as to realize mixing, mixed effect is bad, and efficiency is relatively low, time-consuming.More importantly clear
The link washed, scattered bad, the reactant that the effect deviation that can cause to clean has residual does not wash, and causes result to lose
Very.
The content of the invention
To solve the deficiency in above-mentioned prior art, the invention provides a kind of high sensitivity, detection time are short
Detection means based on microflow control technique.
The purpose of the present invention is achieved through the following technical solutions：
A kind of detection means based on microflow control technique, the detection means based on microflow control technique include the first disk
Piece, first disc have multiple detection units, and detection unit includes detect tank, waste liquid tank；It is described to be based on microflow control technique
Detection means further comprise：
Second disc, second disc are arranged on the lower section of first disc；Second disc has multiple magnetic
Power unit, the magnetic force unit is equal with the quantity of detection unit, and the magnetic force unit includes stopper slot and the first magnetic force piece；Institute
The angle stated between the trend of stopper slot and the radial direction of second disc is acute angle, and first magnetic force piece is arranged on described spacing
In groove；
Second magnetic force piece, second magnetic force piece are fixed on the outer of the center of circle for facing second disc of the stopper slot
Side；Second magnetic force piece keeps geo-stationary with second disc.
The present invention also aims to provide, a kind of detection sensitivity is high, detection time is short based on microflow control technique
The method of work of detection means, the goal of the invention technical scheme is that：
According to the method for work of the above-mentioned detection means based on microflow control technique, the method for work is：
Starting stage：Under the attraction of the second magnetic force piece, the first magnetic force piece, which is in the stopper slot, faces the second disc
The center of circle end position, away from the detect tank on the first disc；
The incubation stage：First disc and the second disc enter low-speed mode, and the first magnetic force piece is adsorbed by the second magnetic force piece
Face the position of the end in the center of circle in the stopper slot；
First disc rotating, scattered magnetic bead move back and forth in stopper slot, and sample, magnetic bead antibody and enzyme are exempted from antibody and mixed
Close and be incubated；
Wash phase：Cleaning fluid enters in detect tank, and the first disc and the second disc enter low-speed mode, the first magnetic force piece
Faced the position of the end in the center of circle in the stopper slot by the absorption of the second magnetic force piece；
First disc rotating, scattered magnetic bead move back and forth in detect tank, and cleaned liquid cleaning；
The waste discharge stage：First disc and the second disc enter fast mode, and the first magnetic force piece is got rid of to the stopper slot
The position of end away from the center of circle, so as to adsorb the magnetic bead in detect tank；
Liquid in detect tank enters in waste liquid tank；
The stage of reaction：Substrate enters in detect tank, and the first disc and the second disc enter the pattern of low speed, the first magnetic force piece
Faced the position of the end in the center of circle in the stopper slot by the absorption of the second magnetic force piece；
First disc rotating, scattered magnetic bead move back and forth in detect tank, and with the substrate reactions.
Compared with prior art, the device have the advantages that being：
1. realizing that different time sections magnet appears in different positions in the presence of centrifugal force and magnetic attracting force, realize
The scattered and aggregation of magnetic bead；
It is high short with flow time that the scattered effect of magnetic bead is mainly reflected in luminous intensity.It is abundant due to mixing, it is being incubated rank
Section, wash phase and substrate reactions stage can shorten the corresponding time, namely shorten detection time, and scattered rear antigen resists
Body joint efficiency height can strengthen the light intensity value under same system, so as to improve sensitivity；
2. in the stage of incubation, wash phase and substrate reactions stage all by way of motor driven disc rotating stirring
Magnetic bead is disperseed into (magnet is away from magnetic bead), so as to realize homogeneous reaction.
Brief description of the drawings
Referring to the drawings, the disclosure will be easier to understand.Skilled addressee readily understands that be：This
A little accompanying drawings are used only for illustrating technical scheme, and are not intended to and protection scope of the present invention is construed as limiting.
In figure：
Fig. 1 is the structure diagram of according to embodiments of the present invention 1 the second disc.
Embodiment
Fig. 1 and following description describe the present invention optional embodiment with instruct those skilled in the art how to implement and
Reproduce the present invention.In order to instruct technical solution of the present invention, simplify or eliminate some conventional aspects.Those skilled in the art should
The understanding is derived from the modification of these embodiments or replacement will within the scope of the invention.Under those skilled in the art should understand that
Stating feature can combine to form multiple modifications of the present invention in a variety of ways.Thus, the invention is not limited in following optional
Embodiment, and only limited by claim and their equivalent.
Embodiment 1：
The structure diagram of the detection means based on microflow control technique of the embodiment of the present invention, it is described based on microflow control technique
Detection means includes：
First disc, first disc are arranged on the top of the second disc, and the second disc has multiple detection units, inspection
Surveying unit includes detect tank, waste liquid tank；The 26S Proteasome Structure and Function of first disc is prior art, be for details, reference can be made to
CN201410669355.X patent application；
Fig. 1 schematically illustrates the structure diagram of the second disc of the embodiment of the present invention, as shown in figure 1, described second
Disc includes：
Multiple magnetic force units, the magnetic force unit is equal with the quantity of detection unit, and the magnetic force unit includes stopper slot 2
With the first magnetic force piece 4；Angle between the trend of the stopper slot and the radial direction of second disc is acute angle, first magnetic force
Part is arranged in the stopper slot；
Second magnetic force piece 3, second magnetic force piece are fixed on the center of circle for facing second disc of the stopper slot
Outside, such as the outside of the end in the close center of circle of stopper slot.
The method of work of detection means based on above-mentioned microflow control technique, the method for work are：
Starting stage：Under the attraction of the second magnetic force piece, the first magnetic force piece, which is in the stopper slot, faces the second disc
The center of circle end position, away from the detect tank on the first disc；
The incubation stage：First disc and the second disc enter low-speed mode, and the first magnetic force piece is adsorbed by the second magnetic force piece
The attraction faced in the stopper slot between the position of the end in the center of circle, namely the first magnetic force piece and the second magnetic force piece carries
The centripetal force needed for the rotation of the first magnetic force piece is supplied；
First disc rotating, scattered magnetic bead move back and forth in stopper slot, and sample, magnetic bead antibody and enzyme are exempted from antibody and mixed
Close and be incubated；
Wash phase：Cleaning fluid enters in detect tank, and the first disc and the second disc enter low-speed mode, the first magnetic force piece
Faced the position of the end in the center of circle in the stopper slot by the absorption of the second magnetic force piece；
First disc rotating, scattered magnetic bead move back and forth in detect tank, and cleaned liquid cleaning；
The waste discharge stage：First disc and the second disc enter fast mode, between the first magnetic force piece and the second magnetic force piece
Attraction is not enough to provide centripetal force of first magnetic force piece at a high speed needed for rotation, and the first magnetic force piece is got rid of to the stopper slot
The position of end away from the center of circle, so as to adsorb the magnetic bead in detect tank；
Liquid in detect tank enters in waste liquid tank；
The stage of reaction：Substrate enters in detect tank, and the first disc and the second disc enter the pattern of low speed, the first magnetic force piece
Faced the position of the end in the center of circle in the stopper slot by the absorption of the second magnetic force piece；
First disc rotating, scattered magnetic bead move back and forth in detect tank, and with the substrate reactions.
It can be seen that during the entire process of above-mentioned incubation stage, wash phase and the stage of reaction, the second magnetic force piece adsorbs
One magnetic force piece so that keep geo-stationary between the first magnetic force piece and the second magnetic force piece.
Embodiment 2：
According to embodiments of the present invention 1 detection means based on microflow control technique and the application examples of method of work.
In the application examples, the first disc has 6 detection units, and accordingly, the second disc has 6 stopper slots,
Stopper slot is linearly, both ends semicircular in shape；First magnetic force piece uses permanent magnet, and the second magnetic force piece is 6, is separately fixed at institute
State on the second disc and curved, matched with the end shape of the stopper slot, and use permanent magnet；Second magnetic force piece exists
Radius of turn under driving is identical, and geo-stationary is kept between the second magnetic force piece and stopper slot (i.e. the second disc).
In the method for work of detection means, in wash phase, after adding cleaning fluid, the second disc jerk of rotation,
First magnetic force piece slides into the position for the end for facing the center of circle in stopper slot, and is attracted by second magnetic force piece.
Embodiment 3：
According to embodiments of the present invention 1 detection means based on microflow control technique and the application examples of method of work.
In the application examples, the first disc has 6 detection units, and accordingly, the second disc has 6 stopper slots,
Stopper slot is linearly；First magnetic force piece uses permanent magnet, and 6 the second magnetic force pieces are joined together to form annular, and using forever
Magnet；Detection means also includes the 3rd disc, and the 3rd disc is arranged between the first disc and the second disc, and the second magnetic force piece is consolidated
It is scheduled on the 3rd disc, under motor driving, the first disc, the second disc and the 3rd disc rotate together, namely the first disc,
Geo-stationary is kept between second disc, the 3rd disc.
In the method for work of detection means, in wash phase, after adding cleaning fluid, the second disc jerk of rotation,
First magnetic force piece slides into the position for the end for facing the center of circle in stopper slot, and is attracted by second magnetic force piece.
Above-described embodiment is only exemplarily to give the situation that the second magnetic force piece uses permanent magnet, certainly, the second magnetic force
Part can also use ferrimagnet.
Claims (8)
1. a kind of detection means based on microflow control technique, the detection means based on microflow control technique includes the first disc,
First disc has multiple detection units, and detection unit includes detect tank, waste liquid tank；It is characterized in that：It is described based on micro-
The detection means of fluidics further comprises：
Second disc, second disc are arranged on the lower section of first disc；Second disc has multiple magnetic force lists
Member, the magnetic force unit is equal with the quantity of detection unit, and the magnetic force unit includes stopper slot and the first magnetic force piece；The limit
Angle between the trend of position groove and the radial direction of second disc is acute angle, and first magnetic force piece is arranged on the stopper slot
It is interior；
Second magnetic force piece, second magnetic force piece are fixed on the end in the center of circle for facing second disc of the stopper slot
Outside；Second magnetic force piece keeps geo-stationary with second disc.
2. the detection means according to claim 1 based on microflow control technique, it is characterised in that：Second magnetic force piece exists
Radius of turn under driving is identical.
3. the detection means according to claim 1 based on microflow control technique, it is characterised in that：The second magnetic force piece group
It is combined into annular.
4. the detection means according to claim 3 based on microflow control technique, it is characterised in that：The detection means enters one
Step includes：
3rd disc, second magnetic force piece are fixed on the 3rd disc；3rd disc is protected with second disc
Hold geo-stationary.
5. the detection means according to claim 1 based on microflow control technique, it is characterised in that：First magnetic force piece is adopted
With magnet, second magnetic force piece uses magnet or ferrimagnet.
6. the detection means according to claim 1 based on microflow control technique, it is characterised in that：Second magnetic force piece with
The quantity of the detection unit is equal.
7. according to the method for work of any described detection means based on microflow control technique of claim 1-6, the work side
Method is：
Starting stage：Under the attraction of the second magnetic force piece, the first magnetic force piece is in the stopper slot circle for facing the second disc
The position of the end of the heart, away from the detect tank on the first disc；
The incubation stage：First disc and the second disc enter low-speed mode, and the first magnetic force piece is adsorbed described by the second magnetic force piece
Face the position of the end in the center of circle in stopper slot；
First disc rotating, scattered magnetic bead move back and forth in stopper slot, sample, magnetic bead antibody and enzyme exempt from antibody mixing incubate
Educate；
Wash phase：Cleaning fluid enters in detect tank, and the first disc and the second disc enter low-speed mode, and the first magnetic force piece is by the
The position of the end in the center of circle is faced in the absorption of two magnetic force pieces in the stopper slot；
First disc rotating, scattered magnetic bead move back and forth in detect tank, and cleaned liquid cleaning；
The waste discharge stage：First disc and the second disc enter fast mode, and the first magnetic force piece is got rid of to remote in the stopper slot
The position of the end in the center of circle, so as to adsorb the magnetic bead in detect tank；
Liquid in detect tank enters in waste liquid tank；
The stage of reaction：Substrate enters in detect tank, and the first disc and the second disc enter the pattern of low speed, and the first magnetic force piece is by the
The position of the end in the center of circle is faced in the absorption of two magnetic force pieces in the stopper slot；
First disc rotating, scattered magnetic bead move back and forth in detect tank, and with the substrate reactions.
8. method of work according to claim 7, it is characterised in that：In wash phase, after adding cleaning fluid, rotation
Second disc jerk, the first magnetic force piece slide into the position for the end for facing the center of circle in stopper slot, and by second magnetic
Power part attracts.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710585320.1A CN107552115B (en) | 2017-07-18 | 2017-07-18 | Detection device based on microfluidic technology and working method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710585320.1A CN107552115B (en) | 2017-07-18 | 2017-07-18 | Detection device based on microfluidic technology and working method thereof |
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| CN107552115B CN107552115B (en) | 2023-06-23 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111925925A (en) * | 2020-08-17 | 2020-11-13 | 鄂州康芯医疗科技有限公司 | Integrated diagnostic kit and application thereof |
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| CN107552115B (en) | 2023-06-23 |
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