CN107552111A - AIDS diagnosis device using hydrophobic material as its substrate of micro-fluidic chip - Google Patents
AIDS diagnosis device using hydrophobic material as its substrate of micro-fluidic chip Download PDFInfo
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- CN107552111A CN107552111A CN201610548253.1A CN201610548253A CN107552111A CN 107552111 A CN107552111 A CN 107552111A CN 201610548253 A CN201610548253 A CN 201610548253A CN 107552111 A CN107552111 A CN 107552111A
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Abstract
The present invention relates to a kind of AIDS diagnosis device using hydrophobic material as its substrate of micro-fluidic chip, belong to analysis testing field.With dimethyl silicone polymer i.e. PDMS that is cheap and easily processing to make AIDS diagnosis with the substrate of micro-fluidic chip be a technical goal.This case main points are that substrate material is the PDMS for having ecosystem surface, and doped micro-scale titanium dioxide particle is filled with its pipeline of chip;The titanium dioxide granule is the titanium dioxide granule that its surface super hydrophilic property can be induced via advance radiation of visible light;The set in its intergranular super hydrophilic gap road of the titanium dioxide granule filler, it is compensatory, surmounted originally strong hydrophobic pipeline, thus reach constant and super hydrophilic modified effect;Meanwhile the difference of interfacial tension is induced at its head and the tail both ends of the chip with the ultrasonic wave of micropower, thereby guide sample liquid stream to be flowed along its pipeline of chip to the terminal direction.
Description
Technical field
The present invention relates to a kind of AIDS diagnosis device using hydrophobic material as its substrate of micro-fluidic chip, the device
It is that analysis testing field is belonged to come the special purpose device of diagnosis of aids based on antigen/antibody specific reaction.
Background technology
The micro-fluidic AIDS diagnosis technical background of associated multi-channel, it is special to may refer to the grade inventions of CN 200910151219.0
Sharp application case.
Only for the microflow control technique overall general picture of itself, famous micro-fluidic expert Mr. Lin Ping Cheng may refer to not
The monograph " diagram Microfluid based Lab on a chip " gone out before long, the monograph is published via Science Press, and the monograph is for miniflow
Past of control technology, now, and, vision of the future etc. etc., suffer from detail, be deep into long opinion of detail
State.
So, the Important Problems of this case that to have a talk below concern.
The basic framework of micro-fluidic chip, including it is etched with the substrate of conduit and the cover plate to fit together therewith, institute
State the fluid course on substrate, before upper cover plate is assembled, it is apparent on see to be exactly some conduits, wait until lid is covered thereon
After piece, just really closure forms the fluid course, and the conduit inner surface of the conduit is together with the part that surround the conduit
Cover plate forms described fluid course together;So, it is clear that assemble the fluid course after completing, its inner surface face
Long-pending major part is the inner surface area of that conduit, and in other words, the state or property of the conduit inner surface are substantially determined
The integrality or property of the fluid course are determined;Therefore say, the inner surface state or interior of this conduit of the structure on substrate
Surface nature is key factor;In principle, any material that can be kept or keep its solid forms substantially, can be used
To make substrate and cover plate, such as, the material that can act as substrate and cover plate can be monocrystalline silicon piece, quartz plate, sheet glass, height
Polymers such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc.;Certainly, the selection of substrate and the choosing of cover plate
Material be able to can also be differed with identical;From the point of view of material consumption, manufacture difficulty and application popularization prospect etc. etc., these
Not small difference, the especially selection of that substrate between material be present, have a great influence.
In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, at this
It is extremely simple that conduit is made on the substrate of sample, and the lower cost for material, substrate is made with the polydimethyl siloxane material,
Conduit is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes,
It is a kind of more satisfactory selection seemingly;Certainly, patch material can also select to use cheap polydimethyl siloxane material:
So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, and making is extremely simple, seems and should also be as
It is extremely easy to popularize, promotes.
But thing is really not so simple.
First, this polydimethyl siloxane material, that is, the material that alphabetical PDMS is referred to of abridging, itself are a kind of strong
Strong hydrophobic material, conduit is built on this material, if without being operated for the modified of the conduit surface, then, it is whole
After body assembling is completed, that is, after covering cover plate, because the conduit its inner surface in structure occupies most fluid course
Inner surface, then, its strong hydrophobic property of the PDMS conduits inner surface, is deciding factor, it can be similar to water
The fine liquid stream of polar liquid of solution by becoming very difficult, its flow resistance is big, or even in general Micropump is all difficult to
Promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar;Therefore, existing
Among having technology, modification is modified particular for the conduit inner surface on the PDMS material, is necessary operation;So, this
The individual modified operation for PDMS conduit inner surfaces is pretty troublesomeThat falls nor this problem, forms serious technical puzzlement,
It is another problem:PDMS polymer molecules inside its body phase of this PDMS material substrate have to be diffused to the surface, moves automatically
The characteristic of shifting, the characteristic that this substrate body phase inside PDMS polymer molecules are diffused to the surface, migrated automatically, will cause by table
State after the modification of its inner surface of that conduit of face modifying and decorating can not maintain the sufficiently long time, and that is through surface
Holding time for conduit its inner surface state after modification is substantially only sufficient to the time needs for completing laboratory internal test experiments;
In other words, by surface is modified or the PDMS conduit inner surfaces of surface modification, after it is modified or institute's shape after modification is said
Into surface state can not be lasting, but soon automatically tend to or say become again surface be modified before surface state, compared with
The strong hydrophobic surface state of that script is returned in the short time, then, just think, such micro-fluidic chip can be big
Amount making, mass storage, it is widely popularized, answer is it is obvious that is, impossible.Conduit on this PDMS material, does not do
If surface modification, it can not pump and pass through similar to the fine liquid stream of polar solvent of the aqueous solution, chip also cannot just use;And such as
Fruit has been cooked surface modification, can not persistently keep its state after modifying again, or equally can not popularization and application.
According to the literature, there is a kind of expedient solution, be that a small amount of surface-active is added in sample to be tested solution
Agent, so as to which during test, dynamic, temporary transient effective surface hydrophilic layer is built temporarily in the PDMS conduits inner surface;
However, due to the amphipathic characteristic of surfactant, the introduced surfactant of the program is inevitable simultaneously also can be with sample solution
In tested composition occur combination so that tested composition can not be detected normally or it is detected ratio and dubiously dropped
It is low;The surfactant is possibly even fully wrapped around by tested composition with micelle form so that tested composition can not be detected or
Identification;Therefore, this kind adds the scheme of surfactant into sample solution, is far from a preferable solution.
So, how to accomplish that substrate can either be made using cheap PDMS material, and the conduit can be released
Inner surface decorating state can not persistently, chip can not largely make, largely lay in and then be widely popularized and such a make this area
The puzzlement that numerous professionals are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.
Be present many year in the highly difficult problem, so far, not yet properly settled.
Second, the PDMS material of non-surface modification, it is stated that, its surface is strongly hydrophobic above, this strong hydrophobic
Material surface and also have another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and
And these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into it is gradually deep, until
It is heavy to be trapped within the body phase of PDMS substrates, in fact, this process, partly it is also due to PDMS material body phase interior polymer
Molecule, which has, to be diffused to the surface, caused by travel motion;Such case, it can also be explained from another angle, i.e. continue not
Disconnectedly from inside PDMS body phases to those polymer molecules of its diffusion into the surface, migration, its result moved, be little by little by that
It has been involved in a bit by the large biological molecule of adsorption within the body phase of PDMS substrates, briefly, these adsorbed biologies
Macromolecular is exactly to be swallowed up by PDMS substrate body phases;So, this PDMS substrates body phase swallows up the phenomenon of large biological molecule, its institute
Caused by influence, necessarily cause the severe deviations of all kinds of test data of experiment for being related to large biological molecule.
As described above, the problem of PDMS substrates, is, its not only adsorption large biological molecule, and swallow up biological big point
Son, so, as the large biological molecule of experiment test object, its disappearance will not stop because surface saturation is adsorbed, and
It is, it is constantly adsorbed, also constantly swallowed up.
On PDMS substrates, its body phase is constantly swallowed up and tests showing for associated biomolecule macromolecular in related experiment test process
As, it is to say that another kind, which is explained, substantial amounts of Minute pores in PDMS body phases be present, associated biomolecule macromolecular by after adsorption,
Depression enters these Minute pores, and then is swallowed up;However, inventor thinks, those can allow the sky of miniature scale
Qi leel squeezes into the Minute pores therebetween, not equal to saying that they also can directly allow the large biological molecule of relative large scale to enter
Enter, both difference on yardstick are huge, must not make sweeping generalizations.Explanation is bypassed, in any case, as dependence test analysis object
Large biological molecule is adsorbed by PDMS substrate conduits inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective
Existing phenomenon.
, can be from containment PDMS surfaces pair in order to prevent swallow up effect of this PDMS substrate bodies relative to large biological molecule
The absorption of large biological molecule addresses, and method is chemically modified modification aiming at the PDMS material surface, for
For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described channel portion, by chemistry
The conduit inner surface of modification, can contain its absorption to large biological molecule, and then avoid large biological molecule quilt
PDMS substrate body phases are swallowed up;But or that old problem, that is, the chemical modification on PDMS material surface is modified it
Surface state afterwards can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface, migrate automatically
Process, can soon by that by surface chemical modification be modified conduit inner surface state become again script it is strong it is hydrophobic simultaneously
And the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS substrates its
Conduit inner surface is always rapidly to strong hydrophobic surface state evolution.
So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, and can is enough
Reach and contain the absorption process of the PDMS substrate conduit inner surfaces to large biological molecule for a long time, and then prevent PDMS substrate bodies relative
The effect of swallowing up of large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life,
It is exactly a very intractable problem.The problem equally makes this area numerous specially as another problem addressed above
Industry personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.Should
Also be present many year in problem, so far, also not yet properly settled.
Third, the polydimethyl siloxane material, namely PDMS material, the active force very little wherein between polymer molecule,
The polymer molecule of low polymerization degree inside its body phase is in constantly into the dynamic process of surface migration, therefore, includes PDMS
After it has been formed, the surface topography in its inner-walls of duct face of chip can be because of described low polymerization degree polymer point for the chip of substrate material
Son moves about, pours into the surface and change, and the change of its pattern is somewhat similarly to Rheological Deformation, trickling deformation or creep and become
Pattern caused by shape institute is possible changes;In the higher PDMS material of some low polymerization degree component ratios, this kind somewhat similar
Can be showed in the phenomenon of Rheological Deformation, trickling deformation or the deformation of creep must be than more significant;This kind include PDMS substrates chip its
The dynamic of inner-walls of duct surface topography after shaping changes property, namely the described property or stream that are somewhat similarly to Rheological Deformation
Dropping down the property of deformation or the property of the deformation of creep, the word of rheology one is applied mechanically in this case, integration, also referred to as the rheological equationm of state or rheology become
Shape property, the dynamic of corresponding inner-walls of duct face pattern change, and this case is also referred to as rheology;As described above, because of this kind of material
Expect certain Rheological Deformation property as described above that its own has, its manufactured goods actually can at leisure Rheological Deformation and cause
The manufactured goods total form generation changes;Influence caused by its Rheological Deformation property, it is to be not easy to feel in terms of macroscopic perspective
Examine, still, for the very fine micro-fluidic chip of its internal structure, the rheological equationm of state can but cause bigger shadow
Ring, the fluid course namely the pipeline therefore, during pre-production formed, because the wall of its tube chamber of pipeline is main
It is the wall of PDMS materials, so, the cross-sectional area of the pipeline actually can gradually become because of rheology over time
It is small, stated differently, since the reason for the rheology, the natively finer pipeline that is flowed through for liquid sample
Its tube chamber the more can become the more narrow, in the case of flow time is sufficiently long, or even the pipeline tube chamber can partly close or all
Ground closes, and then make it that sample fluid course is thoroughly blocked, and closes with being even also not reaching to its luminal part of pipeline
The degree closed or fully closed, its fluid passage that can be provided of that pipeline narrow because of rheology, then, in pipeline
Its inner surface originally be exactly strongly it is hydrophobic in the case of, aqueous sample liquid is just more difficult to by becoming more so because of rheology
Narrow micro-channel, then, the influence of substrate rheology how is resisted, maintains the basic framework of the pipeline not occur seriously to become for a long time
The problem of shape, also, keep the long-term unobstructed of the pipeline is exactly a urgent need to resolve.
The content of the invention
The technical problem to be solved by the invention is to provide a package solution, solves totally above
A series of problems addressed, also, the solution is applied to AIDS diagnosis multichannel micro-fluidic chip field, formed
A kind of new AIDS diagnosis Multichannel device.
The present invention solves the technical problem by following scheme, and the device that the program provides is a kind of with hydrophobic material work
For the AIDS diagnosis device of its substrate of micro-fluidic chip, the structure of the device includes multichannel micro-fluidic chip, the miniflow
The structure of control chip includes being bonded to each other the substrate and cover plate of installing together, and the substrate and cover plate are plate object or sheet
The channel structure via mould pressing process or etching technics formation, the substrate are contained in thing, that face towards the cover plate of the substrate
Also contain and be connected and pierce being formed via mould pressing process, etching technics or simple drilling technology for the substrate with the channel structure
Window structure, be bonded to each other the substrate that is installed together and the cover plate and be built into jointly containing pipeline configuration and therewith
The micro-fluidic chip of connected liquid pool structure, the locations of structures of the pipeline are located at the interface area that the substrate is bonded to each other with the cover plate
Domain, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is exactly the structure bit of the liquid pool
Put, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, remaining
The liquid flowing in its chip when being located at the actual sample introduction test of the micro-fluidic chip of liquid pool its locations of structures terminal, the end
End is located remotely from each other with the sample introduction end, and one end of the pipeline is via the manifold shape turnout for being similarly positioned in the interface zone being bonded to each other
Respectively with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline and the terminal positioned at the micro-fluidic chip
A remaining liquid pool UNICOM, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electricity
Pole and reference electrode, the working electrode is by conductive electrode and is attached on the conductive electrode and has embedded AIDS
The gold size sensitive membrane of specific antibody is formed, and the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is by four
Lateral is in parallel to be formed, and the quantity of the working electrode is four, and the installation position of four working electrodes is located at institute respectively
State in four laterals, and, the specific antibody in its top layer gold size sensitivity membrane structure of four working electrodes is respectively
The four kinds of aids antibody materials that can be specifically bound to AIDS antigen, four kinds of antibody materials are aids antibody respectively
P24 and aids antibody gp41 and aids antibody gp120 and aids antibody gp36, its material of the working electrode
It is gold material or thermal decomposition conducting polymer material, sheet or thread is presented in its pattern of the working electrode, and emphasis is the base
Its material of piece is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the surface of the primary form its
The surface of the primary form for the material for not passing through any surface chemical modification or any surface chemical modification is intended to refer to,
The structure of the device also includes hinge-type fixture, and hinge-type fixture its appearance profile likeness in form hinge, the hinge-type fixture is by phase
Mutually hinged two hinges and through a fastening screw of two hinges and one and the fastening screw
The nut of fastening and hand break loose manually that is used for matched somebody with somebody and connected together with the fastening screw is formed, and the two of the hinge-type fixture
Individual hinge is respectively mutually drawn close with its tip and clamps the micro-fluidic chip, and the tip is positioned at the neighbouring institute of the micro-fluidic chip
State in the locations of structures of terminal, fixation attached in a hinge at least in and is equiped with miniature ultrasonic transducer units,
And higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable and the miniature ultrasonic transducing
Device links together;The hinge-type fixture provides a function of facilitating the device to disassemble;The miniature ultrasonic transducer units its
Major function is in the actual sample introduction test of micro-fluidic chip, utilizes the sample introduction end and the terminal and the miniature ultrasonic
Difference in the distance between transducer installation position difference and its ultrasonic intensity experienced, enter described in induced synthesis
Difference between its interfacial tension of sample end and the terminal its interfacial tension, the interfacial tension between the micro-fluidic chip both ends
Difference can form pressure gap between the both ends of the micro-fluidic chip, and the pressure gap can drive sample solution to the end
End flowing;It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function and also include with it to the strong suction of ultrasonic wave
The property of receipts, ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic chip terminal to limited between the sample introduction end
Short distance within realize the rapid decrement of ultrasonic intensity;And many titanium dioxide granules, many titanium dioxide
It is particles filled in the pipeline, its tube chamber of the pipeline is filled up by many titanium dioxide granules, the titanium dioxide
For its particle size range of grain between 10 microns and 200 microns, the titanium dioxide granule is the titanium dioxide by doping vario-property
Grain, the titanium dioxide granule are its particles of inorganic material of shared percentage by weight more than 80% of titanium dioxide content, the dioxy
It is photic super hydrophilic titanium dioxide granule to change titanium particle, and its function of the titanium dioxide granule includes it can be by receiving in advance
Radiation of visible light forms the titanium dioxide granule surface with super hydrophilic property.
As described above, its composition adulterated of the titanium dioxide granule can be tungsten oxide (WO3), tin oxide (SnO2), five
V 2 O (V2O5), iron oxide (Fe2O3) zinc oxide (ZnO), the silver (Ag) of reduction-state or two or more above-mentioned doping components
Composition;Said composition can be the composition of any ratio of two or more above-mentioned doping components.
Its composition adulterated of the titania particulate material can also include silica (SiO2)。
Titania particulate material described in this case refers to the photic super hydrophilic titania particulate material of visible light-inducing.
Only with regard to the photic super hydrophilic titanic oxide material of various visible light-inducings for itself, for photic super hydrophilic material
It is known for the professional of research field.
Only with regard to the technology of preparing of the photic super hydrophilic titanic oxide material of various visible light-inducings for itself, for photic
It is known for the professional in super hydrophilic investigation of materials field.
Its particle diameter of the titanium dioxide granule can be that the basis between 10 microns and 200 microns is actually needed and any
The particle diameter specified, such as 10 microns, 30 microns, 70 microns, 100 microns, 150 microns or 200 microns of the particle diameter, etc..
The scope of further preferred its particle diameter of the titanium dioxide granule is between 100 microns and 200 microns.
Its pattern of the titanium dioxide granule is unlimited, and the titanium dioxide granule for example can be spheric granules, rod-shaped particles, length
Cube shape particle or any amorphous granular.
The preferred scope of its internal diameter of pipeline is between 500 microns and 1000 microns;It is but excellent compared to above-mentioned
The internal diameter of the pipeline scope of choosing, more tiny internal diameter of the pipeline or thicker internal diameter of the pipeline are also what this case was allowed.
Described miniature ultrasonic transducer units can certainly be all installed in two hinges;But only install one
Individual miniature ultrasonic transducer units are dealt with enough to be used.
The word of hinge one art-recognized meanings of itself are known.
The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and AIDS specific antibody solution uniformly, is used
Point sample instrument point sample is coated on specified structure position, and forms its drying and forming-film.AIDS in the gold size sensitive membrane is special
Heterogenetic antibody is the aids antibody of horseradish peroxidase or glucose oxidase mark, and the gold size sensitive membrane has been wrapped
Containing complementary medium therein is introduced for the above-mentioned each AIDS specific antibody of fixation, the complementary medium such as shell gathers
Sugar, cellulose acetate, gelatin be therein a kind of or their mixture.
The pipeline and the lateral and the manifold shape turnout in the microfluidic chip structure, in it
Footpath size may each be arbitrarily selected size, still, for as far as possible less with prepare liquid sample and reduction reagent loss etc.
Consideration, the pipeline and the lateral and the manifold shape turnout preferably select capillary level passage, institute
The passage for stating capillary level implies that its internal diameter passage suitable with the internal diameter of the capillary on ordinary meaning.The capillary is in it
The shape of cross section of portion's passage can be arbitrary shape, and the shape of cross section is for example circular, oval, square, rectangle, bar
Shape, naturally it is also possible to it is the linear of bending arbitrarily be present, also, the interior shape of the capillary is with the extension of pipeline,
The shape of cross section of different parts can also allow to be different shapes.Only for the word of capillary one, its art-recognized meanings is public
Know.
What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape, which may each be, appoints
The selected shape of meaning, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described
Art-recognized meanings to electrode and the reference electrode vocabulary of itself are known.
It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or capsule for transitional liquid storage
Shape constructs, and its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, and the cavity shape is for example round
Cylindrical cavity shape, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..The size of the liquid pool can be any
Selected size, still, in order to as far as possible less with prepare liquid sample and reduce reagent loss, the liquid pool be preferably capable of with
The liquid pool of the microminiature of capillary matching.
Only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field,
It is known.
Various sizes, variously-shaped ultrasonic transducer are commercially available;Its size of commercially available miniature ultrasonic transducer units
It may diminish to the magnitude only calculated with millimeter.
Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface its
It is known general technology for the professional in ultrasonic technology field for body.This case is not to this expansion superfluous words.
Only with regard to naked PDMS substrates itself conduit molding or lithographic technique for, be open-and-shut known technology;
Similarly, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.
The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.
The structure of the device can also include higher-order of oscillation electric signal generator;The higher-order of oscillation electric signal transmission cable
Its other end can be connected with the higher-order of oscillation electric signal generator.
The involved higher-order of oscillation electric signal generator technology of itself, come for the professional in ultrasonic technology field
Say, be simple and known;The higher-order of oscillation electric signal generator can customize to ultrasonic instrument specialized factory.
The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts
Between;The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with
Between 12MHz.
This case device can further include some annexes certainly, and the annex is such as multiple tracks electrochemical workstation
Deng the art-recognized meanings of the multiple tracks electrochemical workstation are known.The each work being related in this case microfluidic chip structure
Electrode and to electrode and reference electrode etc., special it can get lines crossed and the multiple tracks electrochemical workstation via corresponding respectively
The corresponding interface coupled.It is described that special to get lines crossed be for by each phase of each electrode and the multiple tracks electrochemical workstation
Answer the private cable that interface is coupled to each other.The micro-fluidic chip in this case device, its structure can also include micro-valve,
The quantity of the micro-valve is unlimited, and according to being actually needed, the micro-valve can be installed in any need in the microfluidic chip structure
Position to be mounted;The word of micro-valve one is for the professional of micro fluidic chip technical field, the art-recognized meanings of itself
It is known;The micro-valve itself manufacturing technology and the use of technology is also known;The component that the micro-valve is not required.
The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, still,
It is recommending or say preferable its scope of the diameter between 0.1 micron to 2000 microns;The length of the working electrode can
To allow to be that any setting is easily installed the length used, it is however recommended to or to say the preferable length its scope be 1
Micron is between 15000 microns.
It is installed in by spraying or point sample instrument point sample or the coating of other appropriate process described in the working electrode surface layer
Gold size sensitive membrane, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness,
It is however recommended to thickness preferable thickness is between 10 nanometers and 200 nanometers in other words.
The cover plate in chip structure, its material can allow to be any electrical insulating property material, such as:Polypropylene,
Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, for example do
Into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and realized in the extremely short distance to ultrasonic wave
Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip
Using dimethyl silicone polymer it is used as the cover plate, and this case technical scheme is allowed.
Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should
Air line distance between a remaining liquid pool can be arbitrarily selected suitable distance, still, the distance as needed
Preferred value is between 3 centimetres and 7 centimetres.
Described its thickness of cover plate and substrate can allow be any setting the thickness for being easy to assembling, the thickness of recommendation or say
Preferable thickness is between 1.0 millimeters and 5.0 millimeters.Less thickness is advantageous to save material.
Its maximum difference in application method of this case micro-fluidic chip and general micro-fluidic chip, is exactly this case miniflow
Control chip its before biological detection operation is applied to, it is necessary in advance with the radiation of visible light micro-fluidic chip its pipeline
The titanium dioxide granule of filling;Because micro-fluidic chip is transparent or translucent state in most cases, and due to
The pipeline is fine enough, and its internal titanium dioxide granule filler is also to belong to finer form, it is seen that light also can
Inject with enough penetrating or partial penetration inject fine titanium dioxide granule filler its entity of the form, therefore, can at this
See in the photic super hydrophilic step of photoinduction, directly with its entirety of the radiation of visible light micro-fluidic chip, it is possible to reach and be directed to
Titanium dioxide granule filler inside its pipeline carries out the effect of radiation of visible light;The photic super hydrophilic step of the visible light-inducing
Suddenly can be a few minutes or several days before using this case micro-fluidic chip progress biological detection or more than ten days or twenties
It is completed.
The light irradiation duration of the radiation of visible light step can be a few minutes to more than ten hour.
The lead of the radiation of visible light step, and, the irradiation duration of the radiation of visible light step is, it is necessary to micro- according to this
The species of specific dopant of the titanium dioxide granule filler filled in fluidic chip, the amount of dopant and specific
Its parameters of preparation process and its super hydrophilic state formed retain duration to determine.
After described advance radiation of visible light step is passed through, remaining specific detection of the micro-fluidic chip makes
With step by hereafter sketching;In other words, hereafter refer to using step and method refer to experienced it is advance described
Remaining operation after radiation of visible light.
Itd is proposed first based on this case and the first public new liquid stream driving principle, its application of this case micro-fluidic chip are transported
Among work, the new liquid stream driving method is determined completely without involving any additional Micropump.
The interfacial tension difference that this case is formed between micro-fluidic chip both ends caused by the ultrasonic wave,
Driving liquid stream flows in the capillary channel of the four-way micro-fluidic chip, right respectively using four-way electrochemical analytical instrument
Four kinds of AIDS diagnosis antigens are detected.
The specific detection of this case micro-fluidic chip is as follows using step:
1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various AIDS antigen molecules are each
The AIDS specific antibody for the corresponding horseradish peroxidase-labeled that gold size sensitive membrane embeds is caught on electrode surface in passage
Obtain.
2nd, the AIDS specific antibody of horseradish peroxidase-labeled is formed with the AIDS antigen in blood serum sample and exempted from
Epidemic disease compound.
3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, it is above-mentioned using amperometric detection
Curent change caused by reaction, it is derived from the species and content of various analytes.
4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to AIDS antigen.
It is an advantage of the invention that its close position positions the hinge-type folder in the terminal of the micro-fluidic chip
Tool, installation miniature ultrasonic transducer units are attached in the hinge-type fixture its hinge, are launched with the miniature ultrasonic transducer units low
The ultrasonic wave of power, high-frequency band, meanwhile, using its strong absorbability to ultrasonic wave of dimethyl silicone polymer substrate,
In shorter distance, it is, in from the terminal to the very short distance of only several centimeters yardsticks the sample introduction end,
Reach the rapid decrement of ultrasonic intensity, the difference of the interfacial tension thereby caused at the both ends of the micro-fluidic chip,
And then using the pressure gap between its both ends for being formed of the difference of the interfacial tension between the both ends, drive test liquid
Stream flows in the pipeline to the terminal direction.By this case liquid stream drive scheme, altogether dispense with traditional
The equipment of Micropump etc;The micro-fluidic chip of Micropump is eliminated, its structure is more succinct, and its production process is less, its cost of manufacture
Lower, this kind of succinct structure is more beneficial for the low cost application for reaching micro-fluidic chip.
Its scheme of this case is all to use all clearance spaces in the pipeline in addition to electrode installation takes up space
The titanium dioxide granule fills up.Those are filled in the titanium dioxide granule in pipeline, and this case is to its overall abbreviation titanium dioxide
Granule filler, its function include support, withstand the tube chamber of the pipeline its inwall so that the tube chamber of the pipeline is from because described in
Rheology and narrow, prevent its reduced cross-sectional area of the tube chamber of the pipeline or even close, so as to maintaining the basic of the pipeline for a long time
Serious deformation does not occur for framework.
Numerous titanium dioxide granule entities are mutually banked up under this case framework together at random in the pipeline, the titanium dioxide
The function of titanium granule filler, having for the titanium dioxide granule for also including living through advance radiation of visible light with it certainly are super
The particle surface of hydrophilic nmature, utilize the mutual institute that is mutually close together by ultra-hydrophilic surface of each adjacent titanium dioxide granule
The complications of formation are continuous and its inner surface has the space of super hydrophilic property, in the pipeline Inner Constitution network morphology
Fluid channel with super hydrophilic property, although the fluid channel with super hydrophilic property that this complications can be continuous all the time,
So, inside the tube chamber of the pipeline simultaneously and a plurality of fluid channel with super hydrophilic property deposited its integrate,
The effect of integrated, cumulative superposition in other words, is the equal of that the relatively fine capillary with Superhydrophilic of caliber leads to
Road;Its presence of the continuous super hydrophilic fluid channel of the complications, its Superhydrophilic to a large extent in other words completely,
Thoroughly overcome, surmounted in degree, counteracting the strong hydrophobic property in its surface of the PDMS substrates of the primary form and made
Into the flow resistance for liquid sample;In other words, it is water that because of liquid sample, it, which accounts for the composition of maximum ratio, liquid sample
It is exactly substantially the aqueous solution, therefore, the presence of the titanium dioxide granule filler can be significantly complete, thorough in other words
Overcome in the degree at bottom with the unmodified PDMS substrates of hydrophobic property and surface its to the aqueous solution it is incompatible, repel and
Barrier effect, so be greatly reduced belong to properties of Aqueous Solution sample liquid stream its be advanced through the resistance of pipeline, its flow
Dynamic resistance be possibly even less than or well below the aqueous solution similar caliber the unorganic glass using silica as main component
Flow resistance in capillary.
Under this case framework, inside the tube chamber of the pipeline, the area of its lumen wall wall of former pipeline, because of described two
The rigid of titan oxide particles filler ties up, the table of part hydrophobic property that still can also be exposed on lumen wall wall
Its area of face is already dramatically reduced, and the area on its still exposed part surface for belonging to hydrophobic property is much
Less than the wall surface area of the hydrophobic property of the lumen wall wall in the presence of no titanium dioxide granule filler;
So, under this case framework, in its lumen of the pipeline, its remaining hydrophobic surface and formed after advance radiation of visible light
Super hydrophilic part increases surface newly, and that it is superimposed, comprehensive, summation, cumulative technique effect, is to form on the whole
In super hydrophilic surface;In other words, inside the tube chamber of the pipeline, the part surface of remaining hydrophobic property its with examination
Interfacial tension between sample solution, plus the super hydrophilic part formed after advance radiation of visible light increase newly surface its with
Interfacial tension between sample solution, that it is superimposed, comprehensive, summation, cumulative technique effect, is so that the pipeline
The synergy of its solid-liquid interfacial tension levels off between its tube chamber inner surface of capillary glass tube and sample solution inside tube chamber
Interfacial tension, it is even lower;In other words, as the above-mentioned interfacial tension in this case of the cumulative technique effect actually
Be level off to the aqueous solution and with super hydrophilic property the surface of solids between that interface interfacial tension.
Therefore, exist in the pipeline under this case framework for having stated titanium dioxide granule filler, in advance to core
In the case that its titanium dioxide filler of piece has carried out radiation of visible light, in other words, it is, because micro-fluidic chip is general
For transparent or semitransparent state is integrally presented, therefore, in the case where having carried out radiation of visible light to chip in advance, based on this
The new liquid stream drive scheme of case, it becomes possible to reached with extremely low ultrasonic power by the actuation techniques scheme of this case to examination
The driving of sample liquid stream.
On the other hand, due to its rheological equationms of state of PDMS as substrate material, it is filled in the pipeline
The titanium dioxide granule filler, can gradually by constantly Rheological Deformation and to arest neighbors free space promote PDMS materials
Pipeline wall more tightly wrap up, this process eventually causes those part titanium dioxides for pressing close to the lumen wall wall
Titanium particle is stuck in original place or is stuck in original place or embedding in-situ titanium dioxide granule and remaining embedded in original place, the part
The titanium dioxide granule banked up with being mutually closely packed together each other incarceration together, due to this reason, the dioxy
Change titanium granule filler just will not move in the pipeline easily, and titanium dioxide granule therein is also substantially all locked
In original place, therefore the stream framework of the micro-fluidic chip can be kept for a long time.
The particle size range of its particle of this case titanium dioxide granule filler between 10 microns and 200 microns, compared to
For general organic molecule, general large biological molecule, the particle diameter of its particle of this case titanium dioxide granule filler can be rated as huge
Greatly, due to its huge particle diameter, and the inner surface of the surface of its polarity and the pipeline of strong hydrophobic PDMS materials can not
Mutually perhaps say and mutually melt, then, the huge titanium dioxide granule of this kind of particle diameter can not be by the pipeline of PDMS materials
Surface is adsorbed, and can not more be swallowed up by the inner surface of the pipeline of PDMS materials, the huge titanium dioxide of this kind of particle diameter
Titanium particle surely not be fully sunk in easily or sink to completely PDMS materials its among intrinsic many Minute pores, and
Because above it is stated that the rheology the reason for, the titanium dioxide granule filler can be stuck in original place in other words it is embedding
In original place without moving easily.
Inside the tube chamber of the pipeline, its lumen wall wall of former pipeline is entirely the surface of hydrophobic property originally, such as
Upper described, under this case framework, rigid because of the titanium dioxide granule filler ties up, on lumen wall wall still also
Its area of the surface of part hydrophobic property that can be exposed is already dramatically reduced, and it is still exposed to belong to hydrophobicity
The area on the part surface of matter has been far smaller than the tube chamber in the presence of no titanium dioxide granule filler
The wall surface area of the hydrophobic property of inwall wall;The reason for because of the rheology, it is close to those dioxies of lumen wall wall
Change titanium particle, be little by little partially embedded into or be partly absorbed among the lumen wall wall, the dioxy among the state
Changing titanium particle, it towards the part surface of lumen wall wall and has occurred and that the lumen wall wall that matching deforms is tight
Closely connected conjunction, then, the matching is deformed and closely pasted with titanium dioxide granule surface having occurred and that in lumen wall wall
The part wall of conjunction actually can not contact with flowing through the sample solution of pipeline, that is to say, that the wall base of this part
In sheet can not suction-operated be produced to the large biological molecule therein that flows through the sample solution of pipeline and organic molecule.Base
In above-mentioned mechanism, then because fill up the pipeline the titanium dioxide granule filler its presence, its tube chamber of the pipeline
The overwhelming majority of inwall wall is tightly covered, and these described walls tightly covered substantially can not be with
Flow through the pipeline sample solution contact, only remaining fraction by titanium dioxide granule, its surface is not pasted closely
The hydrophobic surface close, tightly covered may contact with sample solution, in other words, under this case framework, the life in sample solution
Its direct touch opportunity with hydrophobic PDMS walls of thing macromolecular and organic molecule is significantly reduced, thus, PDMS materials
Its adsorptive hindrance and interference of swallowing up to large biological molecule and organic molecule of lumen wall face is greatly reduced;As above institute
State, this case scheme is advantageous to exclude the adsorptive hindrance and swallows up interference, is advantageous to lift the reliable of correlation analysis test data
Property.
As described above, this case titanium dioxide granule filler, its function actually includes being similar to being directed to PDMS materials
Inner surface of pipeline carry out chemical modification effect, functioning as its this respect is by the inner surface of pipeline of the PDMS materials
By the surface modification of hydrophobic property into the surface of super hydrophilic property, still, with general, usual PDMS surface chemical modifications its
The hydrophilic chemical decorative layer retention time is short, hydrophilic effect can not keep situation prolonged enough different, particle described in this case
The huge titanium dioxide granule filler in footpath, its particle being stuck by rheology can not both be moved easily, its huge particle diameter
Particle can not more be swallowed up easily by the inner-walls of duct of PDMS materials, therefore, its from this respect changes similar to surface chemistry
Seen on the technique effect of property, its technique effect of this case, be form it is permanent, can not erase, can not consume, not be corroded, no
The surface reforming layer with super hydrophilic property being swallowed up, being not dissolved, it is just comparable to one kind in PDMS in effect
The permanent modified surface layer with super hydrophilic property built in its relevant surfaces of base material.
It is related that the technical scheme of this case has dissolved its application to dimethyl silicone polymer substrate addressed above totally
A series of technical barriers.Based on this case scheme, the very cheap polydimethyl siloxane material of this kind is just possible to micro- at this
It is prepared by fluidic chip, production, using etc. field play bigger effect.
The miniature ultrasonic transducer units have been installed in fixation in the hinge-type fixture its hinge in this case structure, the structure
A function of facilitating the device to disassemble is provided, in this way, the hinge-type fixture is together with miniature ultrasonic appended in its hinge
Transducer just easily can be mutually disengaged with the micro-fluidic chip, then, the component that the part can freely depart from just can be good
Property is re-used cyclically many times;The architectural feature is advantageous to save the use cost of the device.
Brief description of the drawings
Fig. 1 is the schematic diagram of this case device embodiment chip part construction, it is shown that the vertical view of this structure
The structural form of chip internal pipeline and each electrode and each related liquid pool under angle, the hinge-type is not depicted in the figure
The miniature ultrasonic transducer units set up on fixture and its hinge and other annexes.
Fig. 2 is its rough outside side view of this case device.
In figure, 1,2,10 be the different liquid pool of three installation positions respectively, and 3 be manifold shape turnout, and 4,7,11,14 are respectively
Installation position is different but four laterals parallel with one another for forming UNICOM's structure in parallel, and 5 be its that be installed in lateral 4
The working electrode that specific antibody material in the gold size sensitivity membrane structure of top layer is AIDS specific antibody p24,6 be to be installed in
The work that the specific antibody material in its top layer gold size sensitivity membrane structure in lateral 7 is AIDS specific antibody gp41
Make electrode, 12 be that specific antibody material in its top layer gold size sensitivity membrane structure being installed in lateral 11 is AIDS
Specific antibody gp120 working electrode, 13 be the spy in its top layer gold size sensitivity membrane structure being installed in lateral 14
Heterogenetic antibody material is AIDS specific antibody gp36 working electrode, and 8 be to electrode, and 9 be reference electrode, and 15 be hinge-type
Fixture, 16 sign linkwork positions, 17 be fastening screw, and 18 be higher-order of oscillation electric signal transmission cable, and 19 be miniature
Ultrasonic transducer, 20,25 be two different hinges of locations of structures respectively, and 21 be cover plate, and 22 be substrate, and 23 be sample introduction end,
24 be terminal, and 26 be nut;The hinge-type clamp structure in legend is only the legend structure of signal, actually the hinge-type fixture
Its structure is not limited to the legend hinge-type clamp structure;Arrow in legend indicate the micro-fluidic chip its in actual motion
When, driven by pressure at two ends difference, the flow direction of its sample liquid stream.
Embodiment
In this case that Fig. 1 and Fig. 2 are shown embodiment, the structure of the device includes multichannel micro-fluidic chip, should
The structure of micro-fluidic chip includes being bonded to each other the substrate 22 and cover plate 21 of installing together, and the substrate 22 and cover plate 21 are
What is formed via mould pressing process or etching technics contained in plate object or tablet, that face towards the cover plate 21 of the substrate 22
Channel structure, the substrate 22 also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics
Or the window structure that simple drilling technology is formed, it is bonded to each other the substrate 22 being installed together and is built into jointly with the cover plate 21
Micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the liquid pool are liquid pool 1, liquid pool 2, liquid pool
10, the locations of structures of the pipeline is located at the interface zone that the substrate 22 is bonded to each other with the cover plate 21, and its side of the window is by this
Cover plate 21 blocks and opposite side opens, and the locations of structures of the window is exactly the liquid pool 1, liquid pool 2, the locations of structures of liquid pool 10,
The quantity of the liquid pool is three, and two liquid pools therein are that liquid pool 1 and liquid pool 2 its locations of structures are located at the micro-fluidic chip
Sample introduction end 23, a remaining liquid pool are its chip when liquid pool 10 its locations of structures is located at the micro-fluidic chip actual sample introduction test
The terminal 24 of interior liquid flowing, the terminal 24 are located remotely from each other with the sample introduction end 23, and one end of the pipeline is via being similarly positioned in the phase
The manifold shape turnout 3 for the interface zone being mutually bonded respectively with the liquid pool 1 positioned at sample introduction end 23 and the UNICOM of liquid pool 2, the pipeline
The other end and the remaining liquid pool of the terminal 24 positioned at the micro-fluidic chip be the UNICOM of liquid pool 10, and, according to
Sequence is respectively installed in working electrode in the pipeline on diverse location and to electrode 8 and reference electrode 9, the working electrode
It is made up of conductive electrode and the gold size sensitive membrane for having embedded AIDS specific antibody being attached on the conductive electrode,
The construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of four lateral parallel connections, the work electricity
The quantity of pole is four, and respectively in four laterals, this four work the installation position of four working electrodes
Electrode is working electrode 5, working electrode 6, working electrode 12, working electrode 13 respectively, and, four its top layers of working electrode
Specific antibody in gold size sensitivity membrane structure is the four kinds of aids antibody things that can be specifically bound to AIDS antigen respectively
Matter, four kinds of antibody materials be respectively aids antibody p24 and aids antibody gp41 and aids antibody gp120 and
Aids antibody gp36, for specifically deploying, 5 be in its top layer gold size sensitivity membrane structure being installed in lateral 4
Specific antibody material is AIDS specific antibody p24 working electrode, and 6 be its top layer gold being installed in lateral 7
The working electrode that specific antibody material in glue sensitivity membrane structure is AIDS specific antibody gp41,12 be to be installed in branch
The work that the specific antibody material in its top layer gold size sensitivity membrane structure in pipeline 11 is AIDS specific antibody gp120
Electrode, 13 be that specific antibody material in its top layer gold size sensitivity membrane structure being installed in lateral 14 is special for AIDS
Heterogenetic antibody gp36 working electrode, the working electrode 5, working electrode 6, working electrode 12, working electrode 13 its material are
Gold material or thermal decomposition conducting polymer material, the working electrode 5, working electrode 6, working electrode 12, working electrode 13
Sheet or thread is presented in its pattern, and emphasis is, its material of the substrate 22 is dimethyl silicone polymer material, its surface of substrate 22
The surface of primary form, the surface of the primary form its be intended to refer to and do not pass through any surface chemical modification or any table
The surface of the primary form of the material of face chemical modification, the structure of the device also include hinge-type fixture 15, hinge-type folder
Has 15 its appearance profile likeness in form hinge, the hinge-type fixture 15 is by two hinges 20,25 for being mutually hinged and runs through
One fastening screw 17 of two hinges 20,25 and one match with the fastening screw 17 and are socketed with the fastening screw 17
Together be used to fasten manually and the nut 26 of hand break loose is formed, two hinges 20,25 of the hinge-type fixture 15 respectively with
Its tip is mutually drawn close and clamps the micro-fluidic chip, and the tip is positioned at the neighbouring terminal 24 of the micro-fluidic chip
Attach to fix in locations of structures, in a hinge at least in and be equiped with miniature ultrasonic transducer units, in this legend,
It is to attach to install the miniature ultrasonic transducer units 19 in hinge 20, it is of course also possible to allow all to fill in two hinges 20,25
If upper miniature ultrasonic transducer units, but, changed it is, in general, that attaching one miniature ultrasonic of installing only in a hinge
Energy device is just enough to deal with to be actually needed, and, higher-order of oscillation electric signal transmission cable 18, the higher-order of oscillation electric signal transmission cable
18 one end links together with the miniature ultrasonic transducer units 19;The hinge-type fixture 15 provides one and facilitates the device to tear open
The function of solution;Its major function of the miniature ultrasonic transducer units 19 is in the actual sample introduction test of micro-fluidic chip, using described
The distance between sample introduction end 23 and the terminal 24 and the installation position of miniature ultrasonic transducer units 19 difference and its felt
The difference on ultrasonic intensity being subject to, its interfacial tension of sample introduction end 23 described in induced synthesis and described its interfacial tension of terminal 24
Between difference, the micro-fluidic chip both ends are that the interfacial tension difference between sample introduction end 23 and terminal 24 can be micro-fluidic at this
The both ends of chip are to form pressure gap between sample introduction end 23 and terminal 24, and the pressure gap can drive sample solution to described
Flow in the direction of terminal 24;It is soft and have the substrate 22 of the dimethyl silicone polymer material of elasticity its function and also include with it to super
The property that sound wave absorbs strongly, ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic chip, the terminal 24 arrives the sample introduction
The rapid decrement of ultrasonic intensity is realized within limited short distance between end 23;And many titanium dioxide granules, should
Many titanium dioxide granules are filled in the pipeline, and its tube chamber of the pipeline is filled out by many titanium dioxide granules
Full, for its particle size range of the titanium dioxide granule between 10 microns and 200 microns, the titanium dioxide granule is changed through overdoping
The titanium dioxide granule of property, the titanium dioxide granule are that titanium dioxide content its shared percentage by weight is inorganic more than 80%
Composition granule, the titanium dioxide granule are photic super hydrophilic titanium dioxide granules, and its function of the titanium dioxide granule can including it
The titanium dioxide granule surface with super hydrophilic property is formed by receiving advance radiation of visible light.
Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its try
The flow direction of sample liquid stream.
The miniature ultrasonic for being depicted without attaching installing in Fig. 1 in the hinge-type fixture 15 and one hinge changes
Can device 19 and higher-order of oscillation electric signal transmission cable 18;Also, the higher-order of oscillation telecommunication is depicted without in Fig. 1 and Fig. 2
The associate member such as number generator and multiple tracks electrochemical workstation.
Involved hinge-type fixture 15 can be carried out simply cutting using commercially available hinge, changed a social system;The miniature ultrasonic
Transducer 19 is commercially available;Commercially available miniature ultrasonic transducer units are various in style, can select to use as needed;This is miniature super
Acoustic wave transducer 19 can also customize to ultrasonic transducer specialized factory.
Involved higher-order of oscillation electric signal transmission cable 18 is commercially available;Can also be special to ultrasonic transducer producer or cable
Industry producer customizes.
Involved higher-order of oscillation electric signal generator in the market has the product close to needs commercially available;It can also determine to relevant manufacturers
System.
Each working electrode in this example structure and can be respectively via each special electricity to electrode and reference electrode
Cable or say is being got lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.
Claims (10)
1. using hydrophobic material as the AIDS diagnosis device of its substrate of micro-fluidic chip, the structure of the device includes multichannel
Micro-fluidic chip, the structure of the micro-fluidic chip include being bonded to each other the substrate and cover plate of installing together, the substrate and lid
Piece is plate object or tablet, and that face towards the cover plate of the substrate is contained to be formed via mould pressing process or etching technics
Channel structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics
Or the window structure that simple drilling technology is formed, it is bonded to each other the substrate being installed together and has been built into jointly with the cover plate and contains
The micro-fluidic chip of liquid pool structure for having pipeline configuration and being attached thereto, the locations of structures of the pipeline are located at the substrate and the lid
The interface zone that piece is bonded to each other, its side of the window is blocked by the cover plate and opposite side opens, and the locations of structures of the window is just
It is the locations of structures of the liquid pool, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the miniflow
The sample introduction end of chip is controlled, its locations of structures of a remaining liquid pool is located at when the actual sample introduction of the micro-fluidic chip is tested in its chip
The terminal of liquid flowing, the terminal is located remotely from each other with the sample introduction end, and one end of the pipeline is via being similarly positioned in what this was bonded to each other
The manifold shape turnout of interface zone is micro- with being located at this with the Liang Ge liquid pools UNICOM positioned at sample introduction end, the other end of the pipeline respectively
A remaining liquid pool UNICOM for the terminal of fluidic chip, and, sequentially it is respectively installed in diverse location in the pipeline
On working electrode and to electrode and reference electrode, the working electrode is by conductive electrode and is attached to the electric conductivity
The gold size sensitive membrane for having embedded AIDS specific antibody on electrode is formed, and the construction of the pipeline is in parallel construction, described to be in
The pipeline of parallel construction is made up of four lateral parallel connections, and the quantity of the working electrode is four, four working electrodes
Installation position respectively in four laterals, and, its top layer gold size sensitivity membrane structure of four working electrodes
In specific antibody be the four kinds of aids antibody materials that can be specifically bound to AIDS antigen respectively, four kinds of antibody things
Matter is aids antibody p24 and aids antibody gp41 and aids antibody gp120 and aids antibody gp36 respectively,
Its material of the working electrode is gold material or thermal decomposition conducting polymer material, and sheet is presented in its pattern of the working electrode
It is or thread, it is characterised in that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the table of primary form
Face, the surface of the primary form its be intended to refer to not by any surface chemical modification or any surface chemical modification should
The surface of the primary form of material, the structure of the device also include hinge-type fixture, hinge-type fixture its appearance profile likeness in form
Hinge, the hinge-type fixture by two hinges being mutually hinged and through two hinges a fastening screw with
An and nut for being used for manual fastening and hand break loose for matching with the fastening screw and being connected together with the fastening screw
Form, two hinges of the hinge-type fixture are respectively mutually drawn close with its tip and clamp the micro-fluidic chip, the tip positioning
Fixed dress is attached in the locations of structures of the neighbouring terminal of the micro-fluidic chip, in a hinge at least in
Provided with miniature ultrasonic transducer units, and, higher-order of oscillation electric signal transmission cable, the one of the higher-order of oscillation electric signal transmission cable
End links together with the miniature ultrasonic transducer units;The hinge-type fixture provides a function of facilitating the device to disassemble;
Its major function of the miniature ultrasonic transducer units is in the actual sample introduction test of micro-fluidic chip, utilizes the sample introduction end and institute
State on the distance between terminal and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced
Difference, the difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic core
Interfacial tension difference between the piece both ends can form pressure gap between the both ends of the micro-fluidic chip, the pressure gap
Sample solution can be driven to the end flow;It is soft and have the substrate of the dimethyl silicone polymer material of elasticity its function bag
Include with its property to the strong absorption of ultrasonic wave, ultrasonic wave is absorbed strongly, and thereby in the micro-fluidic chip terminal
The rapid decrement of ultrasonic intensity is realized within to the limited short distance between the sample introduction end;And many titanium dioxide
Particle, many titanium dioxide granules are filled in the pipeline, and its tube chamber of the pipeline is by many titanium dioxide
Grain filled up, its particle size range of the titanium dioxide granule between 10 microns and 200 microns, the titanium dioxide granule be by
The titanium dioxide granule of doping vario-property, the titanium dioxide granule are titanium dioxide content its shared percentage by weights more than 80%
Particles of inorganic material, the titanium dioxide granule is photic super hydrophilic titanium dioxide granule, and its function of the titanium dioxide granule includes
It can form the titanium dioxide granule surface with super hydrophilic property by receiving advance radiation of visible light.
2. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
It is characterised by, the pipeline and the lateral and the manifold shape turnout are capillary channel.
3. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
It is characterised by, its composition adulterated of the titanium dioxide granule is tungsten oxide (WO3), tin oxide (SnO2), vanadic anhydride
(V2O5), iron oxide (Fe2O3) zinc oxide (ZnO), the silver (Ag) of reduction-state or two or more above-mentioned doping components combination
Thing.
4. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
Be characterised by, the width or diameter of the working electrode between 0.1 micron to 2000 microns, and, the working electrode
Length between 1 micron to 15000 microns.
5. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
It is characterised by, the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.
6. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
It is characterised by, the cover plate its material in structure is dimethyl silicone polymer material.
7. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
Be characterised by, positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal should
Air line distance between a remaining liquid pool is between 3 centimetres and 7 centimetres;Described its thickness of cover plate and substrate in structure
Between 1.0 millimeters and 5.0 millimeters.
8. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
It is characterised by, its particle size range of the titanium dioxide granule is between 100 microns and 200 microns, its inside diameter ranges of the pipeline
Between 500 microns and 1000 microns.
9. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
Be characterised by, the structure of the device also includes higher-order of oscillation electric signal generator, the higher-order of oscillation electric signal transmission cable its
The other end is connected with the higher-order of oscillation electric signal generator.
10. the AIDS diagnosis device according to claim 1 using hydrophobic material as its substrate of micro-fluidic chip, its
It is characterised by, for its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units between 5 milliwatts and 9000 milliwatts, this is miniature
The frequency of its ultrasonic wave operationally launched of ultrasonic transducer is between 100KHz and 12MHz.
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CN201610548253.1A CN107552111A (en) | 2016-06-30 | 2016-06-30 | AIDS diagnosis device using hydrophobic material as its substrate of micro-fluidic chip |
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Application publication date: 20180109 |