CN107551330A - Tip biomembrane batch making method - Google Patents
Tip biomembrane batch making method Download PDFInfo
- Publication number
- CN107551330A CN107551330A CN201711028782.XA CN201711028782A CN107551330A CN 107551330 A CN107551330 A CN 107551330A CN 201711028782 A CN201711028782 A CN 201711028782A CN 107551330 A CN107551330 A CN 107551330A
- Authority
- CN
- China
- Prior art keywords
- layer
- making method
- biomembrane
- medicine
- batch making
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Materials For Medical Uses (AREA)
- Prostheses (AREA)
Abstract
The present invention provides tip biomembrane batch making method, comprises the following steps:1)Section is made in advance to be star-like, the model that four drift angles are " V " font and drift angle section and fracture line section coincide;2)Model is immersed to the first solution layer, the second solution layer successively, first solution forms elastic layer, for fine and close degradable or nondegradable biomembrane, second solution layer forms medicine layer, medicine layer material be and the good degraded or non-degradable of human body bone bio-compatible, absorb do not absorb either or porosity and looseness material, be also encapsulated with the medicine for having facilitation to sclerotin healing inside medicine layer;3)After air-drying shape fixation etc. biological microstructure film, size as needed is cut the biological microstructure film made along the oval circumference in model section, and the ring-type biomembrane for cutting later is peeled off from model vertex.
Description
Technical field
Present invention relates to tip biomembrane batch making method.
Background technology
It is attached to currently with the biological composite membrane comprising growth factor/stem cell at fracture line, to strengthen union
Speed and quality, it has been the Disciplinary Frontiers of medical research.Fracture line(Concordant profipole)Blood vessel, osteocyte etc. is distributed with inner surface,
Whether healing is good, depending on blood vessel, the growing state of osteocyte in fracture anastomosis line.Biomembrane is fitted in fracture anastomosis line
Why surface can increase the quality of union, and its reason is the medicine of one side biomembrane encapsulated inside to fracture anastomosis
The growth of blood vessel, osteocyte in line has a facilitation, and another aspect biomembrane is by tissue and fracture such as the muscle in the external world
Concordant profipole is isolated, and prevents reaction of the tissue to blood vessel, bone cell growth in fracture anastomosis line.Therefore, it is biological
Facilitation of the film to union, it should be embodied in and effective medicine and the isolation external world are applied with to fracture anastomosis line inner surface
The tissue such as muscle.
The way of contact of existing biological composite membrane and fracture line is often by the way of being directly bonded, but this kind of mode
Bring problems with:
1, biological composite membrane bulk area is bigger, and fracture area is smaller, and 2 can not effectively be bonded and be fixed on one
Rise.
2, the limitation of current biological composite membrane material and manufacture craft, cause the degradation speed of biological composite membrane, carry
Growth factor release active drug concentration time(The valid density duration is the key of fracture line healing quality)
It can not be met clinical needs etc. performance indications.See《Growth factor slow-release system promotes the experimental study of fracture of mandible healing》;
《Growth factor/collagem membrane slow-released system promotes the research of union》.
Therefore, on the basis of existing biological composite membrane manufacture craft, new structure of composite membrane, and newly compound are explored
The fitting of film and fracture line and fixed form, strengthen the speed and quality of union, into the direction of scientific and technical personnel's innovation.
The content of the invention
Tip biomembrane batch making method, comprises the following steps:
1)Section is made in advance to be star-like, the model that four drift angles are " V " font and drift angle section and fracture line section coincide;
2)Model immerses to the first solution layer, the second solution layer successively, the first solution forms elastic layer, to be fine and close degradable or
Nondegradable biomembrane, the second solution layer form medicine layer, and medicine layer material is and the good drop of human body bone bio-compatible
Solution is non-degradable, absorb do not absorb either or porosity and looseness material, be also encapsulated with to sclerotin healing inside medicine layer
There is the medicine of facilitation;
3)Air-dried etc. biological microstructure film after shape fixes, size as needed is by the biological microstructure film made along mould
The oval circumference in type section is cut, and the ring-type biomembrane for cutting later is peeled off from model vertex.Model is made with waxed,
Wax and elastic layer material are immiscible from each other, are easy to peel off from wax pattern after air-drying.
Further, fracture line section after in vitro people's bone artificial fracture with being made.The different people in fracture line section
It is all similar.Therefore fracture line section after in vitro people's bone artificial fracture with being made.
Further, drift angle section is " V " font and the model identical with fracture line section is cast on fracture line with wax,
Then wax solidification is waited to be formed model later.Wax and sclerotin are also immiscible, drift angle section be " V " font and with fracture line section
Identical model is cast on fracture line with wax, then waits wax solidification to be formed model later, the model is easy to from fracture
Taken off on line.
Further, biological microstructure film periphery is can not permeate the medical bio of the stimulate neuronal growth factor and stem cell
Glue is closed.
Further, the elastic layer material is in PLA, collagen, fibrin, biogel, poly butyric ester
One or several kinds of mixtures.
Further, the elastic layer material is that can not permeate the medical bio of the stimulate neuronal growth factor and stem cell
Glue.
Further, medicine Rotating fields are and human body bone bio-compatible good fiber shape or areolation.
Further, the material of medicine layer is using the material for having facilitation after degraded to bone cell growth.
Further, medicine layer material is that either cellulose or biogel or sterilization or disappear at algal gel azelon
Cytotoxic activity charcoal.
Further, the combination of growth factor either stem cell or growth factor and stem cell is encapsulated with medicine layer.
Further, medicine layer is encapsulated with degradable medicaments slow-released system.
On the structure and material inside each layer of biomembrane, in biomembrane related paper and patent document, discuss
That states is perfectly clear, such as 201510716279.8 a kind of composite repairing materials for being used to bridge defect nerve and its support,
201480055424.6 compositions and delivery system, 201280027492.2 are for regenerative medicine and for tissue supports
Bio-compatible and biodegradable gradient layer system.Section is V-shaped after the application is characterised by its folding.Respectively
Structure and material inside layer, can under the requirement for carrying medicine and delivery system for meeting to be bonded together in the form of biological composite membrane
With from the various structures and material provided in above paper and patent document.
Due to the limitation of biomembrane manufacture craft, the biomembrane one side volume of whole is larger, on the other hand real and bone
The area for rolling over the contact of concordant profipole inner surface depends on the area of fracture anastomosis line inner surface.So only and fracture anastomosis line in table
Medicine on the biomembrane in that block region of face contact can just contact fracture anastomosis line inner surface.Therefore, the application really needs
The technical problem of solution is:
First, how in biomembrane manufacture craft(Each Rotating fields and material)On the Process ba- sis not improved further, fracturing
In the case that concordant profipole inner surface area is constant, the concentration of increase biomembrane release medicine and time;
Second, how preferably biomembrane to be bonded and is fixed on fracture anastomosis line;
Third, how preferably the tissues such as the muscle in the external world and fracture anastomosis line inner surface to be mutually isolated.
For first problem, after fracture operation, fracture anastomosis line(Between between the knochenbruch surface being stitched together
Gap)Formation cross-section is " V " type trench structure.If being directly bonded horizontal biomembrane on the trench structure, now give birth to
Thing film and the area of fracture anastomosis line inner surface contact are only:The length of the width * trench structures of " V " type trench structure upper surface
Degree.If the biomembrane that section itself is also " V " type is filled in the trench structure inner surface, now biomembrane and fracture
The area of concordant profipole inner surface contact is at least:The length * 2 of the depth * trench structures of " V " type trench structure.In ' V ' type groove type
When the depth of the width of structure upper surface " and ' V ' type trench structure " ratio is close to " 1 ", now obvious 2nd kind of biofilm structure
Can be in biomembrane manufacture craft(Each Rotating fields and material)On the Process ba- sis not improved further, in fracture anastomosis line
In the case that surface area is constant, increase biomembrane and the area of fracture anastomosis line inner surface contact.
For Second Problem, later composite biological film is folded, because its internal layer is elastic layer, outer layer is weaker zone
(Medicine layer), therefore later " V " type structure is folded, by the elastic reaction of internal layer elastic layer(Elastic layer material is fine and close, folds
Restoring force is produced later), itself has the trend expanded to both sides.Elastic force caused by certain elastic layer is very little, still
But it is enough interior table of the edge " compression " at left and right sides of the top composite biological film " V " type structure in fracture anastomosis line both sides
Face.
For the 3rd problem, principle is same as above, because the edge " compression " at left and right sides of composite biological film top is being fractured
The inner surface of concordant profipole both sides, hence in so that the tissue such as fracture anastomosis line inner surface and outside muscle obtain effectively every
From.
Simultaneously as the elastic layer material is that can not permeate the Medical Living Creature Gum of growth factor, therefore medicine layer is encapsulated
Medicine, can only be to inner side(Fracture anastomosis line inner surface)Release, can not discharge laterally(The human body tissue sides such as muscle)So that
Limited entrapped drug(One of biomembrane process bottleneck is exactly the amount of entrapped drug can not be too many)Can be with targeted release to fracture
Concordant profipole inner surface, reach the purpose precisely treated.
What is required emphasis is a little that the application uses outer layer as weaker zone(Medicine layer)It is direct with fracture anastomosis line inner surface
The mode of contact, brings a performance more superior than traditional biological film laminating type, i.e. weaker zone can draw in bony surface
Lead osteocyte to grow in the material of porosity and looseness, new osteocyte can also obtain entrapped drug in weaker zone in production process
Effective nourishing and promotion.As for porosity and looseness material in itself, degradable substance can be selected to make, or even further,
Made using the material for having facilitation after degraded to bone cell growth.
What is retrieved when the application submits heals applied to fracture anastomosis line closest to for documents, not retrieving
Foldable structure composite biological film.Expand application field to be retrieved, CN201510716279.8 is due to proposing compound bio
Film using when first pass through folding or convolution formed fibroin layer be internal layer, collagen layer is middle level, high polymer layer is outer layer
Bridge grafting nerves support, belong to application field difference, but disclose more technical characteristic.But it does not still destroy the application
Creativeness.It can be found from CN201510716279.8 accompanying drawing 3, the purpose for playing folding and convolution is by composite biological film
Formed " concentric multi-layer cylinder structure ", change technical characteristic and the application be different, secondly its solve be " will fold or certificate
Support after folding(Multi-layer cylinder structure composite biomembrane)Both ends and the nerve tract both ends blocked are sutured by surgical method "
Its technical problem solved, the technique effect and the application of acquirement are entirely different, therefore do not destroy the creativeness of the application.
Brief description of the drawings
Fig. 1 is schematic diagram of the present invention.
Embodiment
With reference to figure 1, tip biomembrane batch making method, comprise the following steps:
1)Section is made in advance to be star-like, the model 1 that four drift angles are " V " font and drift angle section and fracture line section coincide;
2)Model immerses to the first solution layer, the second solution layer successively, the first solution forms elastic layer, to be fine and close degradable or
Nondegradable biomembrane, the second solution layer form medicine layer, and medicine layer material is and the good drop of human body bone bio-compatible
Solution is non-degradable, absorb do not absorb either or porosity and looseness material, be also encapsulated with to sclerotin healing inside medicine layer
There is the medicine of facilitation;
3)Air-dried etc. biological microstructure film after shape fixes, the edge of biological microstructure film 1 that size as needed will be made
The oval circumference in model section is cut, and the ring-type biomembrane for cutting later is peeled off from model vertex.
Claims (10)
1. tip biomembrane batch making method, comprises the following steps:
1)Section is made in advance to be star-like, the model that four drift angles are " V " font and drift angle section and fracture line section coincide;
2)Model immerses to the first solution layer, the second solution layer successively, the first solution forms elastic layer, to be fine and close degradable or
Nondegradable biomembrane, the second solution layer form medicine layer, and medicine layer material is and the good drop of human body bone bio-compatible
Solution is non-degradable, absorb do not absorb either or porosity and looseness material, be also encapsulated with to sclerotin healing inside medicine layer
There is the medicine of facilitation;
3)Air-dried etc. biological microstructure film after shape fixes, size as needed is by the biological microstructure film made along mould
The oval circumference in type section is cut, and the ring-type biomembrane for cutting later is peeled off from model vertex.
2. tip biomembrane batch making method as claimed in claim 1, it is characterized in that:The in vitro people's bone in fracture line section
It is made after artificial fracture.
3. tip biomembrane batch making method as claimed in claim 2, it is characterized in that:Drift angle section be " V " font and and
The identical model in fracture line section is cast on fracture line with wax, then waits wax solidification to be formed model later, then by mould
Type is taken off from fracture line.
4. tip biomembrane batch making method as claimed in claim 3, it is characterized in that:4)Before, by biological micro-structural
Closed so that the Medical Living Creature Gum of the stimulate neuronal growth factor and stem cell can not be permeated on film periphery.
5. tip biomembrane batch making method as claimed in claim 4, it is characterized in that:The elastic layer material is poly- breast
One or several kinds of mixtures in acid, collagen, fibrin, biogel, poly butyric ester.
6. tip biomembrane batch making method as claimed in claim 5, it is characterized in that:The elastic layer material is to ooze
The Medical Living Creature Gum of saturating the stimulate neuronal growth factor and stem cell.
7. tip biomembrane batch making method as claimed in claim 6, it is characterized in that:Medicine Rotating fields are and human body bone
Bio-compatible good fiber shape or areolation.
8. tip biomembrane batch making method as claimed in claim 7, it is characterized in that:The material of medicine layer use degraded with
There is the material of facilitation to bone cell growth afterwards.
9. tip biomembrane batch making method as claimed in claim 8, it is characterized in that:Medicine layer material be azelon or
Person's cellulose either biogel or sterilization algal gel or antimicrobial activity charcoal.
10. tip biomembrane batch making method as claimed in claim 9, it is characterized in that:Be encapsulated with medicine layer growth because
The combination of sub either stem cell or growth factor and stem cell.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711028782.XA CN107551330A (en) | 2017-10-29 | 2017-10-29 | Tip biomembrane batch making method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711028782.XA CN107551330A (en) | 2017-10-29 | 2017-10-29 | Tip biomembrane batch making method |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107551330A true CN107551330A (en) | 2018-01-09 |
Family
ID=61032620
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711028782.XA Withdrawn CN107551330A (en) | 2017-10-29 | 2017-10-29 | Tip biomembrane batch making method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107551330A (en) |
-
2017
- 2017-10-29 CN CN201711028782.XA patent/CN107551330A/en not_active Withdrawn
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106999635A (en) | Repair of cartilage graft support and its manufacture method | |
CN101897993A (en) | Be used for the granule that soft tissue increases | |
CN111317867A (en) | Nerve conduit and preparation method thereof | |
WO2015040106A1 (en) | Method for the production of structured cellulose patches or elements and devices made using such a method | |
EP2750725A1 (en) | Dental implant, vascular implant and tissue implant made of porous three-dimensional structure of polytetrafluoroethylene | |
CN105944150A (en) | Hydrogel, artificial skin manufactured through application of hydrogel and manufacturing method of artificial skin | |
CN107822744A (en) | A kind of preparation method for the tip composite particles for improving union | |
CN113332312A (en) | Self-forming platelet nano-vesicle based on physical whole particles and preparation method thereof | |
CN107551330A (en) | Tip biomembrane batch making method | |
CN107648678A (en) | Round biomembrane batch making method | |
CN109876197A (en) | A kind of 3D printing skin and preparation method thereof | |
CN107670122A (en) | Round biomembrane batch making mould | |
CN107670123A (en) | Tip biomembrane batch making method | |
CN107625999A (en) | Tip biomembrane batch making mould | |
CN107812246A (en) | Round three layers of biomembrane batch making method | |
CN107670124A (en) | Three layers of biomembrane batch making method of tip | |
CN107617127A (en) | The star-like mould of tip biomembrane | |
CN107626000A (en) | Three layers of biomembrane batch making method of tip | |
CN107812247A (en) | Make the Array Model of round biomembrane | |
CN107823727A (en) | Round biomembrane batch making cross-shaped molds | |
CN107812249A (en) | Round biomembrane cross mould batch making method | |
CN107837128A (en) | A kind of preparation method for the tip biomembrane for improving union | |
CN107812252A (en) | A kind of " W " model production method for the tip biomembrane for improving union | |
CN107773788A (en) | The round biomembrane of star section | |
CN107625997A (en) | Round biological film model array making method |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
WW01 | Invention patent application withdrawn after publication | ||
WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180109 |