CN107549309A - A kind of fruity selenium-enriched cordceps militaris sour milk product and preparation method thereof - Google Patents
A kind of fruity selenium-enriched cordceps militaris sour milk product and preparation method thereof Download PDFInfo
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Abstract
The present invention discloses a kind of fruity selenium-enriched cordceps militaris sour milk product and preparation method thereof, by preparing Cordyceps militaris protoplast, change the cell membrane permeability of cordyceps militaris link bacterial strain, carry out selenium-rich culturing simultaneously, the selenium rich ability of Cordyceps militaris can be significantly improved, again with the defatted fresh milk of low fat and sugar, selenium-enriched cordceps militaris fermentation mycelium homogenate is raw material, and add hawthorn powder, pollen pini, vitamin C etc., carry out mixed fungus fermentation Yoghourt, prepare a kind of low fat and sugar, selenium-enriched cordceps militaris Yoghourt with hypolipemic function, it is single to solve fresh yoghurt function, it is unfavorable for storing, the problems such as diabetes patient can not eat, and strengthen the antilipemic healthy function of Yoghourt, as health food, with preferable development prospect.
Description
Technical field
The present invention discloses a kind of fruity selenium-enriched cordceps militaris sour milk product and preparation method thereof, is changed with a kind of cell membrane permeability
Become and then the selenium-enriched cordceps militaris mycelium that is remarkably reinforced of selenium rich ability and the fresh milk of degreasing is primary raw material, and add hawthorn powder,
The multiple auxiliary materials such as pollen pini solution, sweetener and vitamin, inoculation streptococcus thermophilus, lactobacillus bulgaricus, Lactococcus lactis
The mixed bacteria preparation fermentation of biacetyl subspecies, four kinds of lactobacillus acidophilus, obtains solid sour milk, belongs to fermented yoghourt technical field.
Background technology
Yoghourt is made by milk is fermented, has the nutritive value and absorption efficiency more excellent compared with raw milk.It is multinomial
Research it has been shown that Yoghourt triggered in confrontation by diet and pressure cancer, the plurality kinds of health care nutrition such as increase body fluid yield
Functionally there is prominent effect.And with the rapid development of fermentation technique, in order to increase yoghourt-flavored and mouthfeel, make full use of
Nutritional ingredient in fermentation medium, make fermentation process more abundant, generally use mixed fungus fermentation at present, can more meet different consumption
The demand of person.Although Yoghourt nutritive value is high, it generally adds 7% or so white granulated sugar, and fat content is also very high, so
Can not be by diabetes patient.
Selenium is the essential trace elements of the human body, " the life special effect element " being referred to as in trace element, and in nature, selenium has
Inorganic selenium and organic two states, Organic Selenium exist typically in the form of selenomethionine, follow Methionine metabolism approach to be metabolized, and participate in
The synthesis of albumen, easily store, absorb in tissue；It can be rapidly immediately used by the body after being absorbed by the body, be effectively improved human body
Interior blood selenium situation.The average content of selenium is 0.25mg/kg in adult human body.Human body intake selenium must not be less than 50 μ g/day.The U.S.
The current research result of scientist thinks that Healthy People supplements 200 μ g/ day selenium or appropriate more, can prevent tumour to a certain extent
Generation.But the maximum safe intake of selenium is 400 μ g/day, can be excessively poisoned.In addition, also there is regulation to be immunized, AntiHIV1 RT activity
Etc. virus, protection brain, enhancing male reproductive function, with thyroid function and AITD, septicemia etc.
Critical illness, angiocardiopathy, diabetes are all closely bound up.But Se content is generally relatively low in natural food, only taken the photograph by food
Take selenium element and much can not meet healthy needs come the motion that sustains life.But selenium excess intake can produce anxious, slow to body
Property toxic action, while there is selenium（Sodium selenite）Many side effect effects such as hereditary toxic action, utilize microorganism pair
Inorganic matter sodium selenite（Na2SeO3）Bioconversion is carried out, organic selenium product can be directly obtained, mitigates subsequent treatment pressure.Can be with
The physiologically active and absorptivity of selenium are improved, but also its toxicity can be reduced.
The content of the invention
A kind of low fat and sugar selenium-enriched cordceps militaris Yoghourt with hypolipemic function of disclosure of the invention, can play selenium-rich pupa worm
Grass, hawthorn powder, pollen pini reducing blood lipid, regulation immunity and other effects, have low sugar, the feature of low fat again, in good taste, can be for sugar
Urine patient drinks, and is easy to store and transports, while is also equipped with probiotics etc. and promotes the functions such as gastrointestinal disturbances, is eaten as a kind of health care
Product, there is preferable development prospect.
A kind of preparation method of selenium-enriched cordceps militaris bacterium of the present invention, comprises the following steps：
（1）The preparation of Cordyceps militaris protoplast：By mycelia weight（g）：Enzyme liquid volume（mL）=1:1, add sterile mixed enzyme
Liquid, it is placed in enzymolysis 2.5h in 26 DEG C of shaking table；Centrifuge enzymolysis liquid 6000 r/min, 12 min, abandoning supernatant, steady penetration enhancer cleaning
Precipitation 3 times, centrifuge and thoroughly remove residual enzyme liquid, add steady penetration enhancer vibration, 2500 r/min centrifuge 12 min；Supernatant is taken to centrifuge
12 min, 6000 r/min, protoplast are sunken to centrifuge tube ttom of pipe, add 2 ~ 10 parts of steady penetration enhancer vibration and suspend；
（2）Selenium-riched Cordyceps Militaris culture：Na is added into the Cordyceps militaris protoplast of preparation2SeO3 60 μ g/L, after 26 DEG C stand 8h,
It is transferred in 5% ratio in 100mL Cordyceps militaris regeneration culture mediums, 26 DEG C of vibration (150 r/min) culture 4d；
（3）The mycelial preparation of selenium-enriched cordceps militaris：4000 r/min centrifuge 10 min, obtain mycelium；
（4）It is prepared by selenium-enriched cordceps militaris homogenate：2 times of volume distilled water are added into 100g mycelium, are fully homogenized with colloid mill,
Boil 1~5min inactivations, as selenium-enriched cordceps militaris fermentation homogenate；
（5）The preparation of selenium-enriched cordceps militaris Yoghourt breast：Yoghourt fermentation matrix forms：Selenium-enriched cordceps militaris 1 part~10 parts of homogenate of fermentation,
20 parts~40 parts of fresh cow milk, 5 parts~20 parts of skimmed milk power, 0.2 part~0.5 part of pollen pini, 0.5 part of hawthorn powder, stir, 90
DEG C~95 DEG C of insulation 10 min sterilizations, it is cooled to 40 DEG C, 5 parts~10 parts of inoculating starter, 0.01 part of vitamin C is incubated
Ferment 24 h, when acidity is 60~80 ° of T, and when solidifying complete, terminates fermentation, and taking-up is placed in 4 DEG C, final finished yogurt prod.
Pollen pini of the present invention, be pinaceae plant masson pine, Chinese pine or its congener pollen, be foresythia or
Faint yellow fine powder, sweet taste is nontoxic, is the food of Chinese tradition, various nutrient balances in pollen pini, low in calories rich in fiber,
The aliphatic acid and vitamin contained has synergy, can adjust the cholesterol and blood fat of human body, therefore have reducing blood lipid work(
Effect.Hawthorn is also a kind of health food of traditional medicine-food two-purpose, has reducing blood lipid, strengthening the spleen and stomach and other effects.
The present invention, using enzymatic isolation method, prepares Cordyceps militaris protoplast, changes pupa worm in selenium-enriched cordceps militaris incubation
It careless spore and mycelial cell membrane permeability, can effectively increase the ability that Cordyceps militaris absorbs inorganic selenium, and then improve nothing
Machine selenium biotransformation efficiency, compared to traditional natural absorption training method just with Cordyceps militaris to inorganic selenium, it can promote
The absorption of inorganic selenium, improve transformation efficiency, and reduce the toxic side effect of inorganic selenium, reduce selenium-enriched cordceps militaris production cost and
Cycle.Then the elite of selenium-enriched cordceps militaris, hawthorn and pollen pini is dissolved into Yoghourt preparation technology, there is good health care work(
Effect and edibility.
The positive effect of the present invention is：
The present invention changes the cell membrane permeability of cordyceps militaris link bacterial strain, while carry out selenium-rich by preparing Cordyceps militaris protoplast
Culture, the selenium rich ability of Cordyceps militaris can be significantly improved, then with the defatted fresh milk of low fat and sugar, selenium-enriched cordceps militaris fermentation mycelium
Homogenate is raw material, and adds hawthorn powder, pollen pini, vitamin C etc., carries out mixed fungus fermentation Yoghourt, prepares a kind of low fat and sugar
, there is the selenium-enriched cordceps militaris Yoghourt of hypolipemic function, it is single to solve fresh yoghurt function, is unfavorable for storing, diabetes patient is not
Can be edible the problems such as, and strengthen the antilipemic healthy function of Yoghourt, as health food, there is preferable development prospect.
Embodiment
By following examples further illustrate description the present invention, do not limit the invention in any way, without departing substantially from
On the premise of the technical solution of the present invention, what those of ordinary skill in the art made for the present invention easily realized any changes
Dynamic or change is fallen within scope of the presently claimed invention.
The present embodiments relate to selenium-enriched cordceps militaris and the bacterium culture medium and reagent of Yoghourt fermentation include：
Strain：Cordycepsmilitaris(L.ex Fr.)Link（Carried out from wild cordyceps militaris tissue separation obtain it is pure
Strain is identified by Institute of Microorganism, Academia Sinica）.The double second of streptococcus thermophilus, lactobacillus bulgaricus, Lactococcus lactis
Acyl subspecies, lactobacillus acidophilus zymophyte powder, are purchased from Danisco（China）Co., Ltd.
Cordyceps militaris spawn activation medium：PDA culture medium.
The activation of Cordyceps militaris spawn：The Cordyceps militaris that picking inclined-plane preserves, is inoculated in 100 mL strain activation and culture bases, 26 DEG C
Vibrate (150 r/min) culture 3d.
Cordyceps militaris regeneration culture medium：Glucose 15g/L, peptone 8g/L, yeast extract 10g/L, potassium dihydrogen phosphate 1.5
G/L, epsom salt 1.5 g/L, VB10.1 g/L, the g/L of ammonium sulfate 10, mannitol 0.5mol/L, Na2SeO3 30μg/L。
115 degree of sterilizing 30min.
Steady penetration enhancer（0.5mol/L）：0.5mol/L mannitol, 121 DEG C of sterilizings are configured using pH7.2 phosphate buffer
20min。
Enzyme liquid：Steady penetration enhancer prepares 1% cellulase and 0.5% glusulase, isometric to mix, and 0.22 μm of membrane filtration is degerming.
Leavening forms：Streptococcus thermophilus, lactobacillus bulgaricus, Lactococcus lactis biacetyl subspecies, lactobacillus acidophilus
Four kinds of mixed bacterium bacterium powders, proportionally 1:1:1:1 mixes.
Embodiment 1
（1）The preparation of Cordyceps militaris protoplast：By mycelia weight（g）：Enzyme liquid volume（mL）=1:1, add sterile mixed enzyme
Liquid, it is placed in enzymolysis 2.5h in 26 DEG C of shaking table.Centrifuge enzymolysis liquid 6000 r/min, 12 min, abandoning supernatant, steady penetration enhancer cleaning
Precipitation 3 times, centrifuge and thoroughly remove residual enzyme liquid, add steady penetration enhancer vibration, 2500 r/min centrifuge 12 min.Supernatant is taken to centrifuge
12 min, 6000 r/min, protoplast are sunken to centrifuge tube ttom of pipe, add 2 parts of steady penetration enhancer vibrations and suspend；
（2）Selenium-riched Cordyceps Militaris culture：Na is added into the Cordyceps militaris protoplast of preparation2SeO3 60 μ g/L, after 26 DEG C stand 8h,
It is transferred in 5% ratio in 100mL Cordyceps militaris regeneration culture mediums, 26 DEG C of vibration (150 r/min) culture 4d；
（3）The mycelial preparation of selenium-enriched cordceps militaris：4000 r/min centrifuge 10 min, obtain mycelium；
（4）It is prepared by selenium-enriched cordceps militaris homogenate：2 times of volume distilled water are added into 100g mycelium, are fully homogenized with colloid mill,
Boil 1~5min inactivations, as selenium-enriched cordceps militaris fermentation homogenate；
（5）It is prepared by Yoghourt fermentation matrix：Selenium-enriched cordceps militaris the fermentation kg of homogenate 1, the kg of fresh cow milk 20, the kg of skimmed milk power 5, pine
The kg of pollen 0.2, the kg of hawthorn powder 0.5, stirs, and 90 DEG C~95 DEG C insulation 10 min sterilizations, is cooled to 40 DEG C；
（6）The fermentation of Yoghourt breast：Inoculating starter 5kg, vitamin C 10g, 24 h of incubated fermentation, when acidity be 60~
80 ° of T, and solidify it is complete when, terminate fermentation, taking-up be placed in 4 DEG C, obtain finished yogurt prod.
Embodiment 2
（1）The preparation of Cordyceps militaris protoplast：By mycelia weight（g）：Enzyme liquid volume（mL）=1:1, add sterile mixed enzyme
Liquid, it is placed in enzymolysis 2.5h in 26 DEG C of shaking table.Centrifuge enzymolysis liquid 6000 r/min, 12 min, abandoning supernatant, steady penetration enhancer cleaning
Precipitation 3 times, centrifuge and thoroughly remove residual enzyme liquid, add steady penetration enhancer vibration, 2500 r/min centrifuge 12 min.Supernatant is taken to centrifuge
12 min, 6000 r/min, protoplast are sunken to centrifuge tube ttom of pipe, add 4 parts of steady penetration enhancer vibrations and suspend；
（2）Selenium-riched Cordyceps Militaris culture：Na is added into the Cordyceps militaris protoplast of preparation2SeO3 60 μ g/L, 26 DEG C of standing 8h
Afterwards, it is transferred in 5% ratio in 100mL Cordyceps militaris regeneration culture mediums, 26 DEG C of vibration (150 r/min) culture 4d；
（3）The mycelial preparation of selenium-enriched cordceps militaris：4000 r/min centrifuge 10 min, obtain mycelium；
（4）It is prepared by selenium-enriched cordceps militaris homogenate：2 times of volume distilled water are added into 100g mycelium, it is fully even with colloid mill
Slurry, boil 1~5min inactivations, as selenium-enriched cordceps militaris fermentation homogenate；
（5）It is prepared by Yoghourt fermentation matrix：Selenium-enriched cordceps militaris fermentation homogenate 2kg, fresh cow milk 30kg, skimmed milk power 10kg, preserved egg
The kg of powder 0.3, the kg of hawthorn powder 0.5, stirs, and 90 DEG C~95 DEG C insulation 10 min sterilizations, is cooled to 40 DEG C；
（6）The fermentation of Yoghourt breast：The kg of inoculating starter 7, the kg of vitamin C 0.01,24 h of incubated fermentation, when acidity is
60~80 ° of T, and solidify it is complete when, terminate fermentation, taking-up be placed in 4 DEG C, obtain finished yogurt prod.
Embodiment 3
（1）The preparation of Cordyceps militaris protoplast：By mycelia weight（g）：Enzyme liquid volume（mL）=1:1, add sterile mixed enzyme
Liquid, it is placed in enzymolysis 2.5h in 26 DEG C of shaking table.Centrifuge enzymolysis liquid 6000 r/min, 12 min, abandoning supernatant, steady penetration enhancer cleaning
Precipitation 3 times, centrifuge and thoroughly remove residual enzyme liquid, add steady penetration enhancer vibration, 2500 r/min centrifuge 12 min.Supernatant is taken to centrifuge
12 min, 6000 r/min, protoplast are sunken to centrifuge tube ttom of pipe, add 6 parts of steady penetration enhancer vibrations and suspend；
（2）Selenium-riched Cordyceps Militaris culture：Na is added into the Cordyceps militaris protoplast of preparation2SeO3 60 μ g/L, after 26 DEG C stand 8h,
It is transferred in 5% ratio in 100mL Cordyceps militaris regeneration culture mediums, 26 DEG C of vibration (150 r/min) culture 4d；
（3）The mycelial preparation of selenium-enriched cordceps militaris：4000 r/min centrifuge 10 min, obtain mycelium；
（4）It is prepared by selenium-enriched cordceps militaris homogenate：2 times of volume distilled water are added into 100g mycelium, are fully homogenized with colloid mill,
Boil 1~5min inactivations, as selenium-enriched cordceps militaris fermentation homogenate；
（5）It is prepared by Yoghourt fermentation matrix：Selenium-enriched cordceps militaris the fermentation kg of homogenate 3, the kg of fresh cow milk 35, the kg of skimmed milk power 15, pine
The kg of pollen 0.4, the kg of hawthorn powder 0.5, stirs, and 90 DEG C~95 DEG C insulation 10 min sterilizations, is cooled to 40 DEG C；
（6）The fermentation of Yoghourt breast：The kg of inoculating starter 8, the kg of vitamin C 0.01,24 h of incubated fermentation, when acidity is
60~80 ° of T, and solidify it is complete when, terminate fermentation, taking-up be placed in 4 DEG C, obtain finished yogurt prod.
Embodiment 4
（1）The preparation of Cordyceps militaris protoplast：By mycelia weight（g）：Enzyme liquid volume（mL）=1:1, add sterile mixed enzyme
Liquid, it is placed in enzymolysis 2.5h in 26 DEG C of shaking table；Centrifuge enzymolysis liquid 6000 r/min, 12 min, abandoning supernatant, steady penetration enhancer cleaning
Precipitation 3 times, centrifuge and thoroughly remove residual enzyme liquid, add steady penetration enhancer vibration, 2500 r/min centrifuge 12 min.Supernatant is taken to centrifuge
12 min, 6000 r/min, protoplast are sunken to centrifuge tube ttom of pipe, add 10 parts of steady penetration enhancer vibrations and suspend；
（2）Selenium-riched Cordyceps Militaris culture：Na is added into the Cordyceps militaris protoplast of preparation2SeO3 60 μ g/L, after 26 DEG C stand 8h,
It is transferred in 5% ratio in 100mL Cordyceps militaris regeneration culture mediums, 26 DEG C of vibration (150 r/min) culture 4d；
（3）The mycelial preparation of selenium-enriched cordceps militaris：4000 r/min centrifuge 10 min, obtain mycelium；
（4）It is prepared by selenium-enriched cordceps militaris homogenate：2 times of volume distilled water are added into 100g mycelium, are fully homogenized with colloid mill,
Boil 1~5min inactivations, as selenium-enriched cordceps militaris fermentation homogenate；
（5）It is prepared by Yoghourt fermentation matrix：Selenium-enriched cordceps militaris the fermentation kg of homogenate 10, the kg of fresh cow milk 40, the kg of skimmed milk power 20,
The kg of pollen pini 0.5, the kg of hawthorn powder 0.5, stirs, and 90 DEG C~95 DEG C insulation 10 min sterilizations, is cooled to 40 DEG C；
（6）The fermentation of Yoghourt breast：The kg of inoculating starter 10, the kg of vitamin C 0.01,24 h of incubated fermentation, when acidity is
60~80 ° of T, and solidify it is complete when, terminate fermentation, taking-up be placed in 4 DEG C, obtain finished yogurt prod.
Yoghourt organoleptic quality prepared by 1 ~ embodiment of the embodiment of the present invention 4 is as shown in table 1：
Table 1：
Test example 1
Selenium-rich cordyceps fermented yoghourt anti-fatigue active is studied
Healthy male mice is taken, 5d is adapted to before testing, is randomly divided into 6 groups：1~4 group of Yoghourt embodiment, negative control group（Without richness
Selenium worm grass Yoghourt group）And blank control group（The ml/10g of physiological saline 0.1）, after Yoghourt and 0.2ml water are stirred, continuously
The d of gavage 25, free diet and drinking-water during modeling.After modeling, every group of mouse randomly selects 10, in last feeding Yoghourt 30
After min, the lead of the body weight of tail heel load 5%, mouse is put in the cm of the depth of water 30, the cm of diameter 15, the Plastic Drum that 27 ~ 30 DEG C of water temperature
In, mouse swimming time is recorded, is submerged with mouse head and 8s can not emerge as end mark of swimming again.
Pluck eyeball and take blood, 37 DEG C of standing 30min, serum is fully separated out.Centrifugation（1500 r/min）5 min, collect supernatant measure blood
Clear SOD, MDA value and lactic acid content；Cervical dislocation puts to death mouse, and solution takes liver, physiological saline cleaning, and filter paper blots surface water
After point, a certain amount of liver organization is accurately weighed, adds physiological saline, homogenate, the r/ of refrigerated centrifuge 3000 are organized in ice bath
Min centrifuges 15 min, takes supernatant to determine liver hepatic glycogen content.As a result as shown in 5~table of table 6：
Influence of 5 Yoghourt of the present invention of table to the mice burden swimming time（N=10）
Compared with control group, * * P<0.01, * * * P<0.001.
Influence of 6 Yoghourt of the present invention of table to mouse biochemical indicator vigor（N=10）
Compared with control group, * P<0.05.
As shown in Table 5, the mice burden swimming time can be dramatically increased after Yoghourt feeding, and anti-fatigue active is better than feminine gender
Control group.
Shown by table 6, eat the mouse after Yoghourt compared with blank control group, serum activity of SOD rise, serum MDA contains
Amount reduces, and Serum lactic acid content reduces, and hepatic glycogen content substantially increases, it is seen then that for a long time using selenium-enriched cordceps militaris Yoghourt, Neng Gouti
High mouse anti-reflecting fatigue activity.
Test example 2
The present invention causes the effect for reducing blood fat of hyperlipemia in mice to intraperitoneal injection Triton
Take normal mouse 90, male and female half and half, it is normal feed 3 d after, be randomly divided into 9 groups, i.e., normal group, high blood lipid model group,
Simvastatin group（6 mg/kg）, negative control group（Without selenium-rich cordyceps Yoghourt group）, 1~4 group of Yoghourt embodiment.Except normal group
Outside mouse stomach physiological saline, the modeling of 4 % Triton solution is injected intraperitoneally by 10 ml/kg volume respectively in other 6 groups of mouse,
Control group and model group give isometric physiological saline, other group of gavage Yoghourt, continuous 25 d after injection.In last feeding acid
3 h after milk, mouse pluck eyeball and take blood, separate serum, TC, TG, HDL-C, LDL-C are determined respectively by the explanation of kit.
7 Yoghourt of the present invention of table causes the effect for reducing blood fat of hyperlipemia in mice to intraperitoneal injection Triton（Average value ± SD；n
=10）
Compared with control group, * P<0.05, * * P<0.01, compared with model group, #P<0.05, ##P<0.05.
Test example 3
Effect for reducing blood fat of the Yoghourt of the present invention to bait hyperlipidemia ratses
Normal male SD rats 80 are taken, are randomly divided into 8 groups, every group 10.Control group is fed with normal diet, remaining 6 groups of use
High lipid food（2 % cholesterol, 0.5 % bovine biles, 10% lard, 0.1 % propylthiouracils, the % of basal feed 87.3）Feed,
Give Simvastatin by 10 ml/kg volume gavage respectively simultaneously（6 mg/kg）, negative control group（Without selenium-rich cordyceps Yoghourt
Group）Or 1~4 group of Yoghourt embodiment, normal group and hyperlipidemia model group give normal saline, continuous 28 d, after last feeding
Eyeball takes blood, separation serum measure TC, TG, HDL-C, LDL-C.
Effect for reducing blood fat of 8 Yoghourt of the present invention of table to bait hyperlipidemia ratses（Average value ± SD；n=10）
Compared with control group, * P<0.05, * * P<0.01, compared with model group, #P<0.05.
Claims (2)
1. a kind of preparation method of selenium-enriched cordceps militaris bacterium, comprises the following steps：
（1）The preparation of Cordyceps militaris protoplast：By mycelia weight（g）：Enzyme liquid volume（mL）=1:1, add sterile mixed enzyme
Liquid, it is placed in 2.0 ~ 3.0h of enzymolysis in 24 DEG C ~ 28 DEG C of shaking table；Centrifuge enzymolysis liquid 6000 r/min, 12 min, abandoning supernatant,
Steady penetration enhancer cleaning precipitates 3 times, centrifuges and thoroughly removes residual enzyme liquid, adds steady penetration enhancer and vibrates, 2500 r/min centrifuge 12 min；Take
Supernatant centrifuges 12 min, 6000 r/min, and protoplast is sunken to centrifuge tube ttom of pipe, adds 2 ~ 10 parts of steady penetration enhancer vibrations and suspends；
（2）Selenium-riched Cordyceps Militaris culture：The μ g/L of inorganic selenium sodium selenite 60 are added into the Cordyceps militaris protoplast of preparation, 26 DEG C quiet
After putting 8h, it is transferred in 5% ratio in 100mL Cordyceps militaris regeneration culture mediums, 26 DEG C of vibrations（150 r/min）Cultivate 4d；
（3）The mycelial preparation of selenium-enriched cordceps militaris：4000 r/min centrifuge 10 min, obtain mycelium；
Cordyceps militaris regeneration culture medium：Glucose 15g/L, peptone 8g/L, yeast extract 10g/L, the g/L of potassium dihydrogen phosphate 1.5,
The g/L of epsom salt 1.5 g/L, VB1 0.1, ammonium sulfate 10 g/L, mannitol 0.5mol/L, the μ g/L of sodium selenite 30；115
Degree sterilizing 30min.
2. a kind of preparation method of fruity selenium-enriched cordceps militaris sour milk product,
（1）The 100g mycelium for taking claim 1 to prepare add 2 times of volume distilled water, are fully homogenized with colloid mill, boil 1~
5min is inactivated, and selenium-enriched cordceps militaris fermentation homogenate is made；
（2）It is prepared by Yoghourt fermentation matrix：
By 1 part~10 parts of homogenate of selenium-enriched cordceps militaris fermentation, 20 parts~40 parts of fresh cow milk, 5 parts~20 parts of skimmed milk power, pollen pini
0.2 part~0.5 part, 0.5 part of hawthorn powder stir, 90 DEG C~95 DEG C insulation 10 min sterilization, be cooled to 40 DEG C；
（3）The fermentation of Yoghourt breast：
5 parts~10 parts of inoculating starter, 0.01 part of vitamin C, 24 h of incubated fermentation, when acidity is 60~80 ° of T, and it is solidifying
Gu when complete, terminating fermentation, taking-up is placed in 4 DEG C, produced；
Described leavening is：Streptococcus thermophilus, lactobacillus bulgaricus, Lactococcus lactis biacetyl subspecies, lactobacillus acidophilus
Four kinds of mixed bacterium bacterium powders, proportionally 1:1:1:1 mixes.
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| CN108684824A (en) * | 2018-06-12 | 2018-10-23 | 徐州工程学院 | A kind of Cordyceps militaris fermentation soybean soya-bean milk and preparation method thereof |
| CN111631327A (en) * | 2019-03-01 | 2020-09-08 | 张德珍 | Multi-vitamin beverage containing nano-selenium |
| CN112493412A (en) * | 2020-11-18 | 2021-03-16 | 吉林医药学院 | Slurry silkworm chrysalis bean curd and preparation method thereof |
| CN112868779A (en) * | 2021-02-25 | 2021-06-01 | 李春桐 | Health-care yoghourt containing active ingredients of bacteria |
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| CN111631327A (en) * | 2019-03-01 | 2020-09-08 | 张德珍 | Multi-vitamin beverage containing nano-selenium |
| CN112493412A (en) * | 2020-11-18 | 2021-03-16 | 吉林医药学院 | Slurry silkworm chrysalis bean curd and preparation method thereof |
| CN112868779A (en) * | 2021-02-25 | 2021-06-01 | 李春桐 | Health-care yoghourt containing active ingredients of bacteria |
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