CN107544232A - Holographic imaging apparatus and method and holographic microscope system and method - Google Patents
Holographic imaging apparatus and method and holographic microscope system and method Download PDFInfo
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- CN107544232A CN107544232A CN201610478457.2A CN201610478457A CN107544232A CN 107544232 A CN107544232 A CN 107544232A CN 201610478457 A CN201610478457 A CN 201610478457A CN 107544232 A CN107544232 A CN 107544232A
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Abstract
Present disclose provides a kind of holographic imaging apparatus and method and holographic microscope system and method.The holographic imaging apparatus includes:Microporous element provided with micropore, the light beam for being sent to LED source carry out shaping and filtering, and the LED source is built in the holographic imaging apparatus or is arranged in the handheld terminal of outside;Reflection part, for reflecting shaped and filtering light beam to be incident upon object to be imaged;Load bearing component, for carrying the object to be imaged;And image sensing module, for recording by the transmitted light beam through the object to be imaged and interfering the hologram formed around the diffracted beam of the object to be imaged.
Description
Technical field
This disclosure relates to technical field of imaging, more particularly to a kind of holographic imaging apparatus and method and holography
Microscopic system and method.
Background technology
One important application of optical microscopy is cell observation, and wherein cell is the most base of life entity
This composition structure and minimum basic functional units, cell observation is biology and a kind of base of medical science
This means.However, it is difficult to using common optical microscopy real-time in cell growth process
Blur-free imaging and microstructure and dynamic characteristic research, while its corresponding optical system
Volume and cost also constitute limitation to its extensive use.
For example, phase-contrast micro- (PCM), differential interference contrast micro- (DICM), Hough
Graceful modulation contrast micro- (HMCM) three kinds of phase-contrast microtechnics will by different interference techniques
The phase difference of sample is converted into intensity difference, to strengthen the contrast of sample image, realizes to biological cell
Deng the visualization of transparent sample.Its image taking speed is fast, and is commonly used to the micro- detection of biological cell phase
In.However, the phase of these technologies and the conversion of amplitude are all nonlinear, therefore in imaging only
It can increase and qualitatively display contrast, and the phase that the physical thickness of sample and specific refractivity determine is special
Property can not be come out by these image quantizations.Meanwhile these methods require the optical design of complexity, together
Sample has the requirement of volume and cost.
Directly record is not observed the image of object to Digital holographic microscopy (DHM) technology, but records
Hologram containing the wavefront information for being observed object, then the hologram recorded is entered by computer
Row numerical reconstruction obtains being observed the phase of object and amplitude (light intensity) information, and then completes numeral
Three-dimensionalreconstruction.Compared to above-mentioned common optical microscopy, using the optical system of DHM technologies
Volume can further minimize.Accordingly, there exist the holography improved constantly using DHM technologies
The demand of the performance of imaging device and holographic microscope system.
The content of the invention
The summary that this part provides is to enter in specific embodiment part behind introducing in simplified form
A part for the concept of one step description.It is not intended to the key feature for the theme for determining the disclosure,
It is not intended to limit the scope of the theme of the disclosure.
According to the first aspect of the disclosure, there is provided a kind of holographic imaging apparatus, including:Provided with micropore
Microporous element, carry out shaping and filtering, the hair for the light beam that is sent to LED source
Optical diode light source is built in the holographic imaging apparatus or is arranged on the handheld terminal of outside
In;Reflection part, for reflecting shaped and filtering light beam to be incident upon object to be imaged
On;Load bearing component, for carrying the object to be imaged;And image sensing module, for recording
Interfere by the transmitted light beam through the object to be imaged and around the diffracted beam of the object to be imaged
The hologram of formation.
According to the second aspect of the disclosure, the pore size of the micropore is adjustable.
According to the third aspect of the disclosure, the holographic imaging apparatus also includes:Rotation driving module,
For driving reflection part rotation to change described shaped and filtering light beam described to be imaged
Incident angle on object;And rotation control module, for controlling the behaviour of the rotation driving module
Make.
According to the fourth aspect of the disclosure, the rotation driving module includes:Turntable, at it
The upper installation reflection part;First motor module, the drive of the turntable rotation is driven for generating
Power;And first transmission parts, the driving force for first motor module to be generated are transferred to
The turntable.
According to the 5th of the disclosure the aspect, the holographic imaging apparatus also includes:Micro-displacement Driving mould
Block, for driving described image sensing module to occur on the direction away from or close to the load bearing component
Micro-displacement;And micro-displacement control module, for controlling the operation of the Micro-displacement Driving module.
According to the 6th of the disclosure the aspect, the Micro-displacement Driving module includes:Comprising piezoelectric or
The support member of marmem, for supporting described image sensing module;And driving power,
For being powered or powering off to the piezoelectric or marmem.
According to the 7th of the disclosure the aspect, the Micro-displacement Driving module includes:Second motor module,
The driving force of micro-displacement occurs for generating driving described image sensing module;And second driving section
Part, the driving force for second motor module to be generated are transferred to described image sensing module.
According to the eighth aspect of the disclosure, there is provided a kind of holographic microscope system, including:According to above-mentioned
Holographic imaging apparatus in first to the 7th aspect described in either side;Image pre-processing module, it is used for
Image preprocessing is carried out to the hologram of described image sensing module record;And image reconstruction module,
For from the hologram through image preprocessing, reconstructing the actual pattern of the object to be imaged.
According to the 9th of the disclosure the aspect, described image pretreatment module and image reconstruction module are set
In handheld terminal;The holographic microscope system also includes mobile memory medium, for described in detection
It is installed to during object to be imaged in the holographic imaging apparatus to store described image sensing module record
Hologram, and be installed to when needing to carry out image preprocessing in the handheld terminal.
According to the tenth of the disclosure the aspect, described image pretreatment module and image reconstruction module are set
In handheld terminal;The holographic imaging apparatus and handheld terminal are each provided with communication module, for passing
The hologram of defeated described image sensing module record.
According to the tenth of the disclosure the on the one hand, there is provided a kind of holographic imaging method, including:From luminous
Diode light-source sends light beam;The LED source is sent by the microporous element provided with micropore
Light beam carry out shaping and filtering;Shaped and filtering light beam is reflected so that it is incident by reflection part
Onto object to be imaged;And by image sensing module record by the transmission through the object to be imaged
Light beam and diffracted beam around the object to be imaged interfere the hologram to be formed.
According to the 12nd of the disclosure the aspect, the LED source is built in holographic imaging dress
In the handheld terminal for putting or being arranged on outside.
According to the 13rd of the disclosure the aspect, the pore size of the micropore is adjustable.
According to the fourteenth aspect of the disclosure, the holographic imaging method also includes:By rotation driving mould
Block drives the reflection part rotation to change described shaped and filtering light beam in the thing to be imaged
Incident angle on body.
According to the 15th of the disclosure the aspect, the rotation driving module includes:Turntable, for
The reflection part is installed thereon;First motor module, the turntable rotation is driven for generating
Driving force;And first transmission parts, for the driving force transmission for generating first motor module
To the turntable.
According to the 16th of the disclosure the aspect, the object to be imaged is carried on load bearing component, and
And the holographic imaging method also includes:Existed by Micro-displacement Driving module drive described image sensing module
Micro-displacement occurs on direction away from or close to the load bearing component.
According to the 17th of the disclosure the aspect, the Micro-displacement Driving module includes:Include piezoelectric
Or the support member of marmem, for supporting described image sensing module;And driving electricity
Source, for being powered or powering off to the piezoelectric or marmem.
According to the 18th of the disclosure the aspect, the Micro-displacement Driving module includes:Second motor mould
Block, the driving force of micro-displacement occurs for generating driving described image sensing module;And second transmission
Part, the driving force for second motor module to be generated are transferred to described image sensing module.
According to the 19th of the disclosure the aspect, there is provided a kind of holographic microphotography method, including:According to upper
The step of stating in the 11st to the 18th aspect the holographic imaging method described in either side;It is pre- by image
The hologram that processing module records to described image sensing module carries out image preprocessing;And by image
Module is rebuild from the hologram through image preprocessing, reconstructs the actual pattern of the object to be imaged.
According to the 20th of the disclosure the aspect, described image pretreatment module and image reconstruction module are set
Put in handheld terminal, and the holographic microphotography method also includes:Detecting the object to be imaged
When with mobile memory medium store the hologram that described image sensing module records;And needing to carry out
The mobile memory medium is installed in the handheld terminal during image preprocessing.
According to the 20th of the disclosure the on the one hand, described image pretreatment module and image reconstruction module quilt
It is arranged in handheld terminal, and the holographic microphotography method also includes:Utilize wired or wireless communication
The hologram that described image sensing module records is transferred to the handheld terminal.
Brief description of the drawings
Embodiment of the disclosure is shown in the following figures in a manner of not being limited by citing.Substantially
Ground, the structural representation in these accompanying drawings are not drawn necessarily to scale, but are presented in simplified form each
Feature.
Fig. 1 is the structural representation according to the holographic imaging apparatus of one embodiment of the disclosure;
Fig. 2 is the simplified block diagram according to the holographic microscope system of one embodiment of the disclosure;
Fig. 3 is the flow chart according to the holographic imaging method of one embodiment of the disclosure.
Embodiment
For illustrative purposes, some details are set forth below to provide to disclosed implementation
The thorough understanding of example.However, it will be obvious to a person skilled in the art that these embodiments
It can realize, or can be realized using equivalent in the case where not using these specific details.
At present, the existing imaging device using DHM technologies is usually using LASER Light Source and lens,
So that the structure of imaging device becomes relative complex and visual field is restricted, it is unfavorable for imaging dress
The miniaturization and the raising of imaging efficiency put.Present disclose provides a kind of holographic imaging apparatus and method and
Holographic microscope system and method, it has simplified structure and improved imaging efficiency.Its application field
The conventional detection such as blood cell count, lectin from hemolymph can be included and relevant cell shows disease
Quick inspection of disease etc..Hereinafter, by the holographic imaging apparatus with corresponding embodiment to the disclosure
And method and holographic microscope system and method are specifically described.
I. holographic imaging apparatus
Fig. 1 is the structural representation according to the holographic imaging apparatus of one embodiment of the disclosure.Such as figure
It is shown, according to the holographic imaging apparatus 100 of the embodiment include light emitting diode (LED) light source 102,
Microporous element 104, reflection part 108, load bearing component 112 and image sensing module 114.LED light
Source 102 is used to send the light beam for imaging, and can use any existing (such as commercially available)
LED component.The type for the object to be detected can be depended on, selection is suitable for the thing to the type
The optical wavelength of body imaging, come LED light source used in determining.For example, the sample for fluorescence can be sent
Product, ultraviolet leds light source can be selected.Alternatively, the quantity of LED light source 102 can be one
It is individual or multiple.When the quantity of LED light source 102 is multiple, they can be distributed with being spaced from each other
On the circumference of such as disc-shaped part, and the type for the object to be detected, hand can be depended on
It is dynamic or automatically rotational circle disc-shaped part will be suitable for determining the LED light source of the type image objects
Position is into the microporous element 104 that is described later on of alignment to be imaged so that holographic imaging apparatus 100
As multiduty imaging device.
Microporous element 104 is provided with micropore, for the light beam that is sent to LED light source 102 carry out shaping (or
Limit beam) and filtering.The size of micropore makes shaped and filtering light beam have coherence.As illustrated,
Microporous element 104 is the box-like part with the cavity for housing LED light source 102, its with institute
The one side that the emission side of the LED light source 102 of collecting is corresponding is provided with micropore.It will, however, be evident that
Microporous element 104 can also take any other suitable construction, as long as there is for LED light source
102 light beams sent carry out the micropore of shaping and filtering.For example, microporous element 104 can also
It is provided with the plate-shaped member of micropore.Alternatively, the pore size of micropore can be adjusted manually or automatically, class
It is similar to the iris diaphgram used in such as camera.So, unthreaded hole diameter on the one hand can be adjusted to obtain
Suitably (such as coherence is higher) spot light, on the other hand can control thang-kng amount, and change regards
Wild bright-dark degree.
Reflection part 108 is treated into for reflecting shaped and filtering light beam 106-1 with being incident upon
On picture object 110, and any existing part being capable of reflecting light can be used (for example, plane
Mirror, prism etc.).Alternatively, reflection part 108 can manually or automatically rotate, shaped to change
With the light beam 106-1 of filtering and the object plane where object 110 to be imaged angle, so as to obtain not
With the hologram of incident angle.Therefore, holographic imaging apparatus 100 can include such as rotation driving mould
Block and rotation control module.
Rotation driving module is used to drive reflection part 108 to rotate to change shaped and filtering light beam
Incident angles of the 106-1 on object 110 to be imaged.As an example, rotation driving module can be with
Including turntable, the first motor module and the first transmission parts.Turntable is used in installation reflection thereon
Part 108, and existing (such as commercially available) rotary head can be used (exemplified by its free degree
Such as 1 or 2).First motor module is used for the driving force for generating driving turntable rotation, and can adopt
With existing (such as commercially available) motor (such as stepper motor) and its driver (such as stepping electricity
Machine driver).First transmission parts are used to the driving force of the first motor module generation being transferred to rotation
Platform, and existing (such as commercially available) transmission parts can be used (for example, power transmission shaft, transmission
Gear etc.).As another example, rotation driving module can only include the first power transmission shaft and the first motor
Module.First power transmission shaft can extend along the direction vertical with Fig. 1 paper direction, with reflection part
108 are fixed together, and one end and the output axis connection of the first motor module.So, reflecting part
Part 108 can rotate in the plane parallel with Fig. 1 paper.
Rotation control module is used for the operation (for example, startup, stopping etc.) for controlling rotation driving module.
Rotation control module can have such as default mode and self-defined pattern.In default mode, Xiang Xuan
Turn between the time that drive module is sent between initial time of rotation control signal, each rotation control signal
Every the parameters such as the, angle that rotates every time be preset.In self-defined pattern, these rotation control ginsengs
Number can be set according to the input of user.Rotation control module can be implemented as application specific integrated circuit
(ASIC), field programmable gate array (FPGA) etc., can also be embodied as processor chips.
Load bearing component 112 is used to carry object 110 to be imaged.Object 110 to be imaged can be for example saturating
Bright or translucent sample (for example, biological cell, histotomy, fluid particle etc.).Load bearing component
112 can be transparent or semitransparent at least in the opening position for carrying object 110 to be imaged.As schemed
Show, load bearing component 112 is plate-shaped member.For example, the plate-shaped member can need to place it is to be imaged
Pulled out during object from holographic imaging apparatus 100, and holography is pushed into after object to be imaged has been placed
In imaging device 100.In addition, the plate-shaped member can be provided with the collecting for being used for placing object to be imaged
Portion's (such as groove).It is evident that load bearing component 112 can also take any other suitable construction
(for example, plate-like), as long as object to be imaged can be carried.
As illustrated, incide shaped and filtering the light beam 106-1 mono- on object 110 to be imaged
Part turns into transmitted light beam 106-2 through object 110 to be imaged, and a part bypasses object to be imaged
110 turn into diffracted beam 106-3.Because shaped and filtering light beam 106-1 has coherence, institute
Interfered with transmitted light beam 106-2 and diffracted beam 106-3, so as to form interference pattern i.e. hologram.
Image sensing module 114 is used to record and interfered by transmitted light beam 106-2 and diffracted beam 106-3
The hologram of formation, and can be implemented as such as image sensor array, such as charge coupling device
(CCD) array or complementary metal oxide semiconductor (CMOS) sensor array.Image sensing
Module 114 can be substantially parallel to load bearing component 112 and set.Alternatively, image sensing module 114
Micro-displacement can occur on the direction away from or close to load bearing component 112, to change image sensing mould
The distance between block 114 and load bearing component 112.So, can be obtained into by the regulation of object distance
As the more preferable hologram of effect.Therefore, holographic imaging apparatus 100 can include such as Micro-displacement Driving
Module and micro-displacement control module.
Micro-displacement Driving module is used to drive image sensing module 114 in remote or close load bearing component
Micro-displacement occurs on 112 direction.As an example, Micro-displacement Driving module includes support member
And driving power.Support member is used to support image sensing module 114, and by piezoelectric or shape
Shape memory alloys (SMA) are made.The shapes such as support member can be in the form of a column, helical form, and its
Quantity can be such as one, three or four.Driving power is used to be powered or break to support member
Electricity, micro-displacement is produced so as to make support member occur flexible using piezo-electric effect or shape memory characteristic.
It is evident that support member is not limited to be made up of piezoelectric or SMA completely, as long as including pressure
Electric material or SMA.Correspondingly, in this case, driving power is wrapped into support member
The piezoelectric or SMA contained is powered or power-off.In addition, Micro-displacement Driving module can also use now
(such as commercially available) piezo actor having/SMA acts device to realize.
As another example, Micro-displacement Driving module can include the second motor module and the second driving section
Part.Second motor module is used to generate the driving force that micro-displacement occurs for driving image sensing module 114,
And existing (such as commercially available) motor (such as ultrasound electric machine) and its driver (example can be used
Such as ultrasonic motor driver).Second transmission parts are used to transmit the driving force of the second motor module generation
To image sensing module 114, and existing (such as commercially available) transmission parts (example can be used
Such as, power transmission shaft, travelling gear etc.).
The operation that micro-displacement control module is used to control Micro-displacement Driving module is (for example, starting, stopping
Deng).Micro-displacement control module can have such as default mode and self-defined pattern.In default mode,
To Micro-displacement Driving module send initial time of micro-displacement control signal, each micro-displacement control signal it
Between the parameter such as time interval, the voltage/current size that is powered every time and time be preset.Making by oneself
In adopted pattern, these micro-displacement control parameters can be set according to the input of user.Micro-displacement controls
Module can be implemented as application specific integrated circuit (ASIC), field programmable gate array (FPGA) etc.,
Processor chips can also be embodied as.Micro-displacement control module and rotation control module can be realized in phase
On same or different circuit board, or it can realize in identical or different processor chips.
In addition, rotation control module was not only included in holographic imaging apparatus 100 but also had controlled mould including micro-displacement
In the case of block, holographic imaging apparatus 100 can alternatively include main control module, for making rotation
Control module and micro-displacement control module co-operatively work.For example, it is in reflection part 108
During a certain angle, micro-displacement control module can control Micro-displacement Driving module to make image sensing module 114
The one or more displacements of generation, can reset image sensing module 114 after the completion of imaging;Then,
Rotation control module can control rotation driving module reflection part 108 is rotated to next angle, micro-
Bit andits control module can control Micro-displacement Driving module image sensing module 114 is occurred one again
Or multiple displacements, can reset image sensing module 114 after the completion of imaging;By that analogy, no
Repeat again.Main control module can be implemented as application specific integrated circuit (ASIC), field-programmable gate array
(FPGA) etc. is arranged, processor chips can also be embodied as.In addition, rotation control module, microbit
Moving control module and main control module can realize on identical or different circuit board, or can be real
In now identical or different processor chips.
It should be noted that the holographic imaging apparatus of the disclosure is not limited to embodiment described above.Example
Such as, holographic imaging apparatus can also not include LED light source.In such a case, it is possible to utilize outside
Handheld terminal (such as mobile phone, tablet personal computer, personal digital assistant etc.) in LED flash come
The light beam of imaging is provided.Now, micropore can be arranged on the shell of holographic imaging apparatus.Needing
When being imaged, the LED flash in handheld terminal can be opened, and on alignment device shell
The micropore of setting.So, the structure of holographic imaging apparatus can be further simplified, and apparatus body
Product can further be reduced.Crust of the device can be optionally provided with dust cap, for not needing
Micropore is covered when being imaged.
II. holographic microscope system
Fig. 2 is the simplified block diagram according to the holographic microscope system of one embodiment of the disclosure.In figure
Dotted line frame represents selectable unit (SU)/module.As illustrated, the holographic microscope system 200 according to the embodiment
Including holographic imaging apparatus 100, image pre-processing module 216 and image reconstruction module 218.It is holographic into
As device 100 includes LED light source 102, microporous element 104, reflection part 108, load bearing component
112nd, image sensing module 114, rotation driving module, rotation control module, Micro-displacement Driving module
With micro-displacement control module, wherein LED light source 102, rotation driving module, rotation control module,
Micro-displacement Driving module and micro-displacement control module are selectable unit (SU)/modules.These part/modules are above
In have been carried out being described in detail, will not be repeated here.
Image pre-processing module 216 is used to carry out image to the hologram that image sensing module 114 records
Pretreatment.Image preprocessing can use high-pass filtering method to realize the contrast enhancement of hologram, improve
Diffraction efficiency, the influence of zero-order image is eliminated, or digital subtraction method, gray scale can also be used linearly to become
Change method scheduling algorithm.Image pre-processing module 216 can be implemented as application specific integrated circuit (ASIC), show
Field programmable gate array (FPGA) etc., can also be embodied as processor chips.
Image reconstruction module 218 is used for the hologram through image preprocessing, reconstructs object to be imaged
Actual pattern.By rebuilding, the amplitude and phase information of object to be imaged can be extracted, wherein,
Amplitude information react bright-dark degree, and the phase information after unpacking can react object to be imaged (such as
Cell) positional information, so as to restore the three-dimensional appearance of object to be imaged, internal structure etc.
Information.For example, cytoplasm is relatively difficult to see in amplitude information, but cytoplasmic phase information is very bright
Aobvious.Both information combine, nucleocytoplasmic ratio, the change of internal albumen available for measure cell, growth
Situation etc..Image reconstruction module 218 can use conventional digital hologram algorithm for reconstructing (for example, luxuriant and rich with fragrance
Nie Er converter techniques, angular spectrum method, convolution method), some new algorithms can also be used (for example, GS
Algorithm, FastICA etc.).
Alternatively, comprise at least rotation driving module in holographic imaging apparatus 100 and its accordingly control mould
, can be with the case of block (alternatively, in addition to Micro-displacement Driving module and its corresponding control module)
Obtain the hologram of the different incidence angles of same object to be imaged.Image pre-processing module 216 and image
Multiple phase informations of object to be imaged can be gone out according to several hologram reconstructings by rebuilding module 218, be led to
These phase informations and corresponding incident angle are crossed to correct the calculation error caused by noise.For example,
Then phase shift or other operations can be carried out according to the difference of incident angle, optical path difference caused by calculating.
Image pre-processing module 218 can be implemented as application specific integrated circuit (ASIC), field-programmable gate array
(FPGA) etc. is arranged, processor chips can also be embodied as.Image pre-processing module 216 and image
Rebuilding module 218 can realize on identical or different circuit board, or can realize identical or
In different processor chips.
As an example, image pre-processing module 216 and image reconstruction module 218 can be realized
In computing device including processor, memory and communication interface.It is stored with memory for real
The computer program of the function of existing image pre-processing module 216 and image reconstruction module 218.Work as processing
When device performs the computer program, processor can serve as image pre-processing module 216 and image weight
Model block 218.Communication interface can take any existing data transmission interface (for example, wired biography
Defeated interface or wireless transmission interface).Computing device can pass through the communication interface and holographic imaging apparatus
Image sensing module is connected, for gathering the hologram recorded by image sensing module.The calculating is set
The standby display (such as liquid crystal display) that can also include is rebuild with showing by image reconstruction module 218
Micro-image.So, it is only necessary to a computing device and holographic imaging apparatus, it is possible to biology
Cell is counted, classified, health status is analyzed (for example, by means of upper layer software (applications)) etc..
As another example, image pre-processing module 216 and image reconstruction module 218 can be arranged on
In handheld terminal (for example, mobile phone, tablet personal computer, personal digital assistant etc.).In the handheld terminal
Memory in be stored with work(for realizing image pre-processing module 216 and image reconstruction module 218
The computer program of energy, it can take such as mobile phone to apply the form using (app).Work as processing
When device performs the computer program, processor can serve as image pre-processing module 216 and image weight
Model block 218.In this case, holographic microscope system can also include mobile memory medium (example
Such as SD card), passed for being installed to when detecting object to be imaged in holographic imaging apparatus with storage image
Feel the hologram of module record, and be installed to when needing to carry out image preprocessing in handheld terminal.Make
For a kind of alternative, holographic imaging apparatus and handheld terminal can be each provided with communication module (for example,
Wire communication module or wireless communication module), the hologram for image sensing module to be recorded is transferred to
Image pre-processing module.So, by being combined with handheld terminal, it may be constructed low-power consumption, portable
Medical or family's detection platform, particularly complex environment the easy-to-use detection of formula.It should be noted that work as
When providing the light beam of imaging using the LED flash of handheld terminal, the handheld terminal is with performing image
Pretreatment and the handheld terminal rebuild can be same handheld terminal or different handheld terminals.
III. holographic imaging method
Fig. 3 is the flow chart according to the holographic imaging method of one embodiment of the disclosure.First, exist
Step 302, light beam is sent from LED source.As it was previously stated, LED source can be with
It is built in holographic imaging apparatus or is arranged in the handheld terminal of outside.Then, in step
304, the light beam sent by the microporous element provided with micropore to LED source carries out shaping and filter
Ripple.As it was previously stated, the pore size of micropore is adjustable.
Then, in step 306, shaped and filtering light beam is reflected so that it is incident by reflection part
Onto object to be imaged.Alternatively, holographic imaging method can also include:Driven by rotation driving module
Dynamic reflection part is rotated to change incident angle of shaped and filtering the light beam on object to be imaged.
Then, in step 308, recorded by image sensing module by the transmitted light through object to be imaged
Beam and diffracted beam around object to be imaged interfere the hologram to be formed.Alternatively, holographic imaging side
Method can also include:Treated by Micro-displacement Driving module drive image sensing module away from or close to carrying
Micro-displacement occurs on the direction of the load bearing component of imaging object.On LED source, micropore portion
Part, reflection part, load bearing component, image sensing module, rotation driving module and Micro-displacement Driving mould
Block, because part i above has been carried out being described in detail, so will not be repeated here.
IV. holographic microphotography method
In addition to comprising the step of holographic imaging method described in III part above, according to this
The holographic microphotography method of disclosed embodiment also includes:By image pre-processing module to image sensing module
The hologram of record carries out image preprocessing;And by image reconstruction module from through the complete of image preprocessing
Breath figure, reconstruct the actual pattern of object to be imaged.
Alternatively, image pre-processing module and image reconstruction module can be arranged in handheld terminal.
In this case, can also include as an example, holographic microphotography method:It is to be imaged detecting
The hologram recorded during object with mobile memory medium storage image sensing module;And needing to carry out
Mobile memory medium is installed in handheld terminal during image preprocessing.It is aobvious as another example, holography
Micro- method can also include:The hologram for being recorded image sensing module using wired or wireless communication is passed
It is defeated by handheld terminal.
On image preprocessing step, image reconstruction step and above-mentioned optional step, due to above
Part ii have been carried out being described in detail, so will not be repeated here.
Based on described above, the holographic imaging apparatus and method and holographic microscope system of the disclosure and side
Method can advantage is obtained that:
(1) nondestructively detection can be carried out to cell to be imaged.Compared in general microscope, also provide
Quantitative phase information.More cell structure informations can be extracted according to this.
(2) imaging optical path is simplified.Due to not using lens so that imaging device need not
Complicated optical imagery structurally and mechanically device, so as to simplify structure, reduce volume, realization device
Miniaturization, portability.
(3) baroque LASER Light Source is replaced by the use of weak relevant LED as light source, makes holographic imaging
Device is able to further simplification, while can reduce speckle noise.
(4) due to without lens, so visual field is bigger, can be once to pair of larger area
As being observed.This application (such as classified counting of leucocyte, HIV detection etc.) to requiring imaging efficiency
It is critically important.
The disclosure include herein by it is clear and definite or it is any it is general in a manner of any novel spy for describing
Sign or combinations of features.In the case where described above has been read in conjunction with the figure, to the above-mentioned of the disclosure
The various modifications or change of embodiment will be apparent to those skilled in the art.It is all these
Modification or change will be fallen within the scope of the disclosure.In addition, described above is only the example of the disclosure
Property embodiment, and the protection domain of the disclosure is not intended to limit, the protection domain of the disclosure is by institute
Attached claim determines.
Claims (21)
1. a kind of holographic imaging apparatus, including:
Microporous element provided with micropore, for the light beam that is sent to LED source carry out shaping and
Filtering, the LED source are built in the holographic imaging apparatus or are arranged on outside
Handheld terminal in;
Reflection part, for reflecting shaped and filtering light beam to be incident upon object to be imaged
On;
Load bearing component, for carrying the object to be imaged;And
Image sensing module, for recording by the transmitted light beam through the object to be imaged and around institute
The diffracted beam for stating object to be imaged interferes the hologram to be formed.
2. holographic imaging apparatus according to claim 1, wherein the pore size of the micropore is
It is adjustable.
3. holographic imaging apparatus according to claim 1 or 2, in addition to:
Rotation driving module, for driving the reflection part rotation to change described shaped and filtering
Incident angle of the light beam on the object to be imaged;And
Control module is rotated, for controlling the operation of the rotation driving module.
4. holographic imaging apparatus according to claim 3, wherein the rotation driving module bag
Include:
Turntable, for installing the reflection part thereon;
First motor module, the driving force of the turntable rotation is driven for generating;And
First transmission parts, the driving force for first motor module to be generated are transferred to the rotation
Turntable.
5. according to the holographic imaging apparatus any one of claim 1-4, in addition to:
Micro-displacement Driving module, for driving described image sensing module in the remote or close carrying
Micro-displacement occurs on the direction of part;And
Micro-displacement control module, for controlling the operation of the Micro-displacement Driving module.
6. holographic imaging apparatus according to claim 5, wherein the Micro-displacement Driving module bag
Include:
Support member comprising piezoelectric or marmem, for supporting described image to sense mould
Block;And
Driving power, for being powered or powering off to the piezoelectric or marmem.
7. holographic imaging apparatus according to claim 5, wherein the Micro-displacement Driving module bag
Include:
Second motor module, the driving of micro-displacement occurs for generating driving described image sensing module
Power;And
Second transmission parts, the driving force for second motor module to be generated are transferred to the figure
As sensing module.
8. a kind of holographic microscope system, including:
According to the holographic imaging apparatus any one of claim 1-7;
Image pre-processing module, it is pre- that the hologram for being recorded to described image sensing module carries out image
Processing;And
Image reconstruction module, for from the hologram through image preprocessing, reconstructing the thing to be imaged
The actual pattern of body.
9. holographic microscope system according to claim 8, wherein described image pretreatment module and
Image reconstruction module is arranged in handheld terminal;And
Wherein described holographic microscope system also includes mobile memory medium, for described to be imaged in detection
It is installed to during object in the holographic imaging apparatus to store the holography of described image sensing module record
Figure, and be installed to when needing to carry out image preprocessing in the handheld terminal.
10. holographic microscope system according to claim 8, wherein described image pretreatment module
It is arranged on image reconstruction module in handheld terminal;And
Wherein described holographic imaging apparatus and handheld terminal are each provided with communication module, described for transmitting
The hologram of image sensing module record.
11. a kind of holographic imaging method, including:
Light beam is sent from LED source;
The light beam sent to the LED source by the microporous element provided with micropore carry out shaping and
Filtering;
Shaped and filtering light beam is reflected by reflection part to be incident upon on object to be imaged;With
And
Treated by image sensing module record by the transmitted light beam through the object to be imaged and around described
The diffracted beam of imaging object interferes the hologram to be formed.
12. holographic imaging method according to claim 11, wherein the LED source
It is built in holographic imaging apparatus or is arranged in the handheld terminal of outside.
13. the holographic imaging method according to claim 11 or 12, wherein the hole of the micropore
Footpath size is adjustable.
14. the holographic imaging method according to any one of claim 11-13, in addition to:
The reflection part as described in rotation driving module drive is rotated to change described shaped and filtering light
Incident angle of the beam on the object to be imaged.
15. holographic imaging method according to claim 14, wherein the rotation driving module bag
Include:
Turntable, for installing the reflection part thereon;
First motor module, the driving force of the turntable rotation is driven for generating;And
First transmission parts, the driving force for first motor module to be generated are transferred to the rotation
Turntable.
16. the holographic imaging method according to any one of claim 11-15, wherein described treat
Imaging object is carried on load bearing component;And
Wherein described holographic imaging method also includes:
By Micro-displacement Driving module drive described image sensing module in the remote or close load bearing component
Direction on micro-displacement occurs.
17. holographic imaging method according to claim 16, wherein the Micro-displacement Driving module
Including:
Support member comprising piezoelectric or marmem, for supporting described image to sense mould
Block;And
Driving power, for being powered or powering off to the piezoelectric or marmem.
18. holographic imaging method according to claim 16, wherein the Micro-displacement Driving module
Including:
Second motor module, the driving of micro-displacement occurs for generating driving described image sensing module
Power;And
Second transmission parts, the driving force for second motor module to be generated are transferred to the figure
As sensing module.
19. a kind of holographic microphotography method, including:
The step of holographic imaging method according to any one of claim 11-18;
The hologram recorded by image pre-processing module to described image sensing module carries out image in advance
Reason;And
By image reconstruction module from the hologram through image preprocessing, the object to be imaged is reconstructed
Actual pattern.
20. holographic microphotography method according to claim 19, wherein described image pretreatment module
It is arranged on image reconstruction module in handheld terminal;And
Wherein described holographic microphotography method also includes:
Described image sensing module is stored when detecting the object to be imaged with mobile memory medium to record
Hologram;And
The mobile memory medium is installed to the handheld terminal when needing to carry out image preprocessing
In.
21. holographic microphotography method according to claim 19, wherein described image pretreatment module
It is arranged on image reconstruction module in handheld terminal;And
Wherein described holographic microphotography method also includes:
The hologram that described image sensing module records is transferred to the hand using wired or wireless communication
Hold terminal.
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