CN107541523B - Varicose streptothricin biosynthesis gene cluster and application thereof - Google Patents

Abstract

The invention discloses a varicose streptothricin biosynthesis gene cluster and application thereof. The whole gene cluster contains 41 genes: 8 genes involved in the biosynthesis of 3-amino-5-hydroxybenzoic acid (AHBA), a precursor of varicocele streptothricin; 5 genes related to the biosynthesis of the carbon skeleton of varicose streptothricin; 28 genes associated with post-biosynthetic modification of varicose streptothricin. The biosynthesis of the varicella streptothricin can be blocked or improved by genetic manipulation of the biosynthesis genes. The gene and the protein thereof provided by the invention can be used for genetic engineering, protein expression, enzyme catalytic reaction and the like of the compound, and can also be used for searching and discovering compounds or genes and proteins which can be used for medicine, industry or agriculture.

Description

Varicose streptothricin biosynthesis gene cluster and application thereof

Technical Field

The invention belongs to the field of microbial gene resources and genetic engineering, and particularly relates to cloning, sequence analysis, in-vivo gene function verification, in-vitro biochemical research and application of a biosynthetic gene cluster of a naphthoansha antibiotic varicocele streptothricin (Streptovaricin).

Background

The structure of the varicose streptothricin is that the varicose streptothricin is a naphthalene ansamycin antibiotic formed by connecting an aromatic ring and a long-chain fatty acid. The biosynthesis of the NANA-Sha antibiotics such as the varicose streptothrin is that 3-amino-5-hydroxybenzoic acid (AHBA) is used as a common starting unit, acetate and propionate are used as basic extension units under the catalysis of polyketide synthase to gradually form a basic skeleton of the compounds such as the varicose streptothrin, and then the varicose streptothrin is subjected to abundant post-modification under the catalysis of related post-modification genes to finally form a series of varicose streptothrin with different structural characteristics.

Biological activity tests show that the varicose streptothricin has better bacteriostatic activity on staphylococcus aureus, and simultaneously has better inhibitory action on Methicillin-resistant staphylococcus aureus (MRSA). Currently, infections caused by MRSA are seriously threatening the health of humans, and for this reason, it is of utmost importance to find and obtain effective anti-MRSA active compounds from nature.

Disclosure of Invention

In order to overcome the defects in the prior art, the invention takes the gene which is commonly used by the naphthalene ansamycin antibiotics and is responsible for AHBA biosynthesis as a target, and locates a gene cluster which is possibly responsible for the biosynthesis of the streptothricin from the genome sequence of Streptomyces spp XW-1(Streptomyces spp XW-1 with the preservation number of CCTCCNO: M2017417), wherein the chemical structure of the streptothricin is shown in figure 1. Cloning the gene cluster for biosynthesis of the streptothricin, and finally positioning the gene cluster responsible for biosynthesis of the streptothricin in the genome of the streptomyces XW-1 (with the preservation number of CCTCC NO: M2017417) through sequence analysis, functional verification and gene knock-out and complementation experiments.

The whole gene cluster comprises 41 genes, the nucleotide sequence of which is shown as 1-95304 th site in SEQ ID NO.1, wherein the genes responsible for biosynthesis of a varicose-streptothricin precursor AHBA, namely, stvG, stvH, stvI, stvJ, stvK, stvL, stvM and stvN, are 8 genes; the genes responsible for carbon skeleton synthesis of the varicose streptothricin, namely 5 genes of stvA, stvB, stvC, stvD and stvE; genes stvA1, orf1309, orf1308, orf1307, orf1306, orf1305, orf1304, orf1303, orf1302, orf1301, stvO1, stvO2, stvO3, stvP1, stvM1, stvP2, stvA2, stvP3, stvF, stvP4, stvO4, stvP5, stvM2, stvM3, orf1272, stvR1, orf1270, stvR2 which are responsible for post-modification of streptothricin constitute a total of 28 genes.

The invention also provides a nucleotide sequence for coding 3-DHQ synthase, the coded amino acid sequence is SEQ ID NO.2 and is named as stvG, and the nucleotide sequence is positioned at 76251-77324 basic groups in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding DAHP synthase, the coded amino acid sequence is SEQ ID NO.3 and is named as stvH, and the nucleotide sequence is positioned at 77495-78649 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence coding shikimate dehydrogenase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.4, named stvI, and the nucleotide sequence is positioned at the 78657-th and 79469-th bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding AHBA synthase, the coded amino acid sequence is SEQ ID NO.5 and is named as stvK, and the nucleotide sequence is positioned at the 79685-80845 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding oxidoreductase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.6 and is named as stvL, and the nucleotide sequence of the nucleotide sequence is positioned at the 81127-81942 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding phosphoglycolic acid phosphatase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.7 and is named as stvM, and the nucleotide sequence of the nucleotide sequence is positioned at 82095-82679 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding glucokinase, the coded amino acid sequence is SEQ ID NO.8 and is named stvN, and the nucleotide sequence is positioned at 82699-83652 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding dehydratase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.9 and is named as stvJ, and the nucleotide sequence is positioned at 94975-94424 base of SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding polyketide synthase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.10 and is named as stvA, and the nucleotide sequence is positioned at 20415-37088 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding polyketide synthase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.11 and is named as stvB, and the nucleotide sequence of the nucleotide sequence is positioned at 37120-47883 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding polyketide synthase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.12 and is named as stvC, and the nucleotide sequence of the nucleotide sequence is positioned at 48086-58798 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding polyketide synthase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.13 and is named stvD, and the nucleotide sequence is positioned at the 58958-64522 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding polyketide synthase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.14 and is named as stvE, and the nucleotide sequence of the nucleotide sequence is positioned at the 64592-75304 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding acyltransferase, the coded amino acid sequence is SEQ ID NO.15 and is named stvA1, and the nucleotide sequence is positioned at 348-707 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the aryl sulfatase, the coded amino acid sequence is SEQ ID NO.16 and is named as orf1309, and the nucleotide sequence is positioned at the 740-th and 1354 th bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding unknown protein, the coded amino acid sequence is SEQ ID NO.17 and is named as orf1308, and the nucleotide sequence is positioned at 1380-1703 th base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence coding for 6-phosphogluconate dehydrogenase, the coded amino acid sequence is SEQ ID NO.18 and is named as orf1307, and the nucleotide sequence is positioned at 3161-1722 th base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the acetylxylan esterase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.19 and is named as orf1306, and the nucleotide sequence of the nucleotide sequence is positioned at 3335-4306 bases of SEQ ID NO. 1.

The invention also provides a nucleotide sequence for encoding peptidase, the encoded amino acid sequence is SEQ ID NO.20 and is named orf1305, and the nucleotide sequence is positioned at 4428-5765 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the membrane protein, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.21 and is named as orf1304, and the nucleotide sequence of the nucleotide sequence is positioned at 7074-5854 th base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the ferric iron reductase, the coded amino acid sequence is SEQ ID NO.22 and is named as orf1303, and the nucleotide sequence is positioned at the 7347-8063 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding glycosyltransferase, wherein the coded amino acid sequence is SEQ ID NO.23 and is named as orf1302, and the nucleotide sequence is positioned at 8239-9288 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the glucose-1-phosphoadenosine acyltransferase, the coded amino acid sequence is SEQ ID NO.24 and is named as orf1301, and the nucleotide sequence is positioned at the 9379-10599 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding NADP dependent dehydrogenase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.25 and is named stvO1, and the nucleotide sequence of the nucleotide sequence is positioned at 10846-11814 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding oxidoreductase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.26 and is named as stvO2, and the nucleotide sequence of the nucleotide sequence is positioned at 11823-th and 12806-th bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the NADPH dependent reductase, the coded amino acid sequence is SEQ ID NO.27 and is named stvO3, and the nucleotide sequence is positioned at 12889 th and 13452 th bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding cytochrome P450, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.28 and is named stvP1, and the nucleotide sequence of the nucleotide sequence is positioned at 13481-14773 th base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding methyltransferase, the coded amino acid sequence of which is SEQ ID NO.29 and is named stvM1, and the nucleotide sequence of which is positioned at 15159-15767 base of SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding cytochrome P450, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.30 and is named as stvP2, and the nucleotide sequence of the nucleotide sequence is positioned at 15800-17005 th base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the fatty acyl transferase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.31 and is named stvA2, and the nucleotide sequence is positioned at 18275-17106 th base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding cytochrome P450, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.32 and is named as stvP3, and the nucleotide sequence of the nucleotide sequence is positioned at 18765-19958 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding amide synthase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.33 and is named as stvF, and the nucleotide sequence of the nucleotide sequence is located at 75408-76202 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding cytochrome P450, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.34 and is named stvP4, and the nucleotide sequence of the nucleotide sequence is positioned at the 83977-85221 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding propionate hydroxylase, the coded amino acid sequence is SEQ ID NO.35 and is named as stvO4, and the nucleotide sequence is positioned at bases 86934-85273 in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding cytochrome P450, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.36 and is named stvP5, and the nucleotide sequence of the nucleotide sequence is positioned at 87228-88481 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding SAM-dependent methyltransferase, the coded amino acid sequence of which is SEQ ID NO.37 and is named stvM2, and the nucleotide sequence of which is positioned at 89660-88593 bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the methyltransferase, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.38 and is named as stvM3, and the nucleotide sequence is positioned at the 90540-th and 89737-th bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the ferroxyl enzyme, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.39 and is named as orf1272, and the nucleotide sequence of the nucleotide sequence is positioned at the 90982-th and 90785-th bases of SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the regulatory factor, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.40 and is named as stvR1, and the nucleotide sequence is positioned at 92612-th and 91314-th bases in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding unknown protein, the coded amino acid sequence is SEQ ID NO.41 and is named as orf1270, and the nucleotide sequence is positioned at 92941-one 93300 base in SEQ ID NO. 1.

The invention also provides a nucleotide sequence for coding the regulatory factor, the coded amino acid sequence of the nucleotide sequence is SEQ ID NO.42 and is named as stvR2, and the nucleotide sequence is positioned at bases 93785-94414 in SEQ ID NO. 1.

In another aspect of the present invention, there is provided a gene of a cytochrome p450 gene cluster dependent on coenzyme ferredoxin and ferredoxin reductase, which is any one of stvP1, stvP2, stvP3, stvP4 and stvP5, and the nucleotide sequences thereof are represented by bases 13481-14773, 15800-17005, 18765-19958, 83977-85221 and 87228-88481 in SEQ ID NO:1, respectively.

Further, there is provided an application of any one of StvP1, StvP2, StvP3, StvP4 and StvP5 genes of coenzyme ferredoxin and ferredoxin reductase-dependent cytochrome p450 family genes in catalyzing biosynthesis of streptothricin and its derivatives.

Starting from cloning a biosynthesis gene cluster, the biosynthesis is researched by adopting a method combining microbiology, molecular biology, biochemistry and organic chemistry, and the biosynthesis pathway of the streptothricin is disclosed by researching the biosynthesis mechanism of the streptothricin. On the basis, the principle of metabolic engineering is applied, and a novel medicine which is stable in structure, better in activity and capable of being produced by mass fermentation of microorganisms is explored through reasonable modification of a biological synthesis way by combining biology.

Applications of the inventive varicose streptothricin biosynthesis gene cluster include (but are not limited to):

(1) the invention also provides a way of producing microorganisms having an interruption in the biosynthetic gene cluster of varicose streptothricin, at least one of the genes comprising the nucleotide sequence of SEQ ID NO. 1.

(2) The cloned DNA comprising the nucleotide sequence provided by the invention or at least part of the nucleotide sequence can be used for positioning more library plasmids from a streptomyces XW-1 (with the preservation number of CCTCC NO: M2017417) genome library. These library plasmids contain at least a partial sequence of the present invention.

(3) Comprising the nucleotide sequence provided by the present invention or at least part of the nucleotide sequence may be modified or mutated. These include insertions, substitutions or deletions, polymerase chain reaction, error-mediated polymerase chain reaction, site-specific mutations, reconnection of different sequences, directed evolution of different parts of a sequence or homologous sequences from other sources, or mutagenesis by ultraviolet light or chemical reagents, and the like.

(4) Cloned genes comprising the nucleotide sequences provided by the invention or at least part of the nucleotide sequences can be expressed in an exogenous host by means of a suitable expression system to obtain the corresponding enzymes or other higher biological activity or yield. Such foreign hosts include Streptomyces, Pseudomonas, Escherichia, Bacillus, yeast, plants, and animals.

(5) The amino acid sequences provided by the invention can be used for separating the required protein and can be used for preparing antibodies.

(6) Polypeptides comprising the amino acid sequences or at least partial sequences provided herein may have biological activity, even new biological activity, after removal or substitution of certain amino acids, or increased yield or optimized protein kinetics or other properties sought to be achieved.

(7) Genes or gene clusters comprising the nucleotide sequences or at least part of the nucleotide sequences provided by the present invention can be expressed in heterologous hosts and their function in the metabolic chain of the host is understood by DNA chip technology.

(8) Genes or gene clusters comprising the nucleotide sequences or at least part of the nucleotide sequences provided by the present invention can be genetically recombined to construct recombinant plasmids to obtain novel biosynthetic pathways, or can be inserted, substituted, deleted or inactivated to obtain novel biosynthetic pathways.

(9) The protein encoded by the nucleotide sequence provided by the invention can catalyze AHBA to synthesize the varicosity streptothricin, and can be recombined with the biosynthesis pathway or partial biosynthesis pathway of other natural products to obtain the novel ansamycin compounds.

Therefore, a recombinant vector, an expression cassette, a transgenic cell line or a recombinant bacterium containing the gene cluster are also within the protection scope of the present invention.

The application of the protein, the gene cluster, the recombinant vector, the expression cassette, the transgenic cell line or the recombinant bacteria in the synthesis of the streptothricin is also the protection scope of the invention.

Another object of the present invention is to provide a method for synthesizing varicose streptothricin, which comprises fermenting the recombinant bacteria, collecting the fermentation product, and obtaining the varicose streptothricin series compounds.

In conclusion, the invention provides all gene and protein information related to the biosynthesis of the varicose streptothricin, which can help people to understand the biosynthesis mechanism of the naphthalene ansamycin antibiotics and provide materials and knowledge for further genetic modification. The gene and the protein thereof provided by the invention can also be used for searching and discovering compounds or genes and proteins which can be used for medicine, industry or agriculture.

Drawings

FIG. 1: the chemical structure of streptothricin;

wherein the compounds 1-6 are the varicose streptothricin existing in the fermentation product of streptomyces XW-1 (the preservation number is CCTCC NO: M2017417).

FIG. 2: A) a gene structure diagram of the gene cluster for the biosynthesis of varicella-streptothricin. B) Schematic interruption diagram of a biosynthesis gene cluster of the streptothricin and High Performance Liquid Chromatography (HPLC) analysis of a fermentation product, wherein the wild-type is a wild-type streptomyces XW-1 (preservation number is CCTCC NO: m2017417), and the genes of Δ stvP1, Δ stvP2, Δ stvP3, Δ stvP4 and Δ stvP5 are single-gene knockout mutants of genes stvP1, stvP2, stvP3, stvP4 and stvP5 in the genome of Streptomyces XW-1 respectively.

FIG. 3: schematic representation of the biosynthetic pathway of streptothricin.

Detailed Description

The features and advantages of the present invention will be further understood from the following detailed description taken in conjunction with the accompanying drawings. The examples provided are merely illustrative of the method of the present invention and do not limit the remainder of the disclosure in any way.

The experimental procedures used in the following examples are conventional unless otherwise specified.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

1. Cloning and analyzing a biosynthetic gene cluster of the varicose streptothricin:

the total DNA of streptomyces XW-1 (preservation number is CCTCC NO: M2017417) is extracted firstly, and the whole genome scanning sequencing is carried out on the total DNA by utilizing a sequencing technology. The obtained total genome sequence is subjected to online data analysis, and through the calculation of AntiSMASH, all possible expressed secondary metabolic gene clusters existing in the whole genome are basically determined, wherein the secondary metabolic gene clusters comprise a biosynthesis gene cluster of the Nagasa antibiotics. Through further data analysis, we determined that the naphthoansamycin biosynthesis gene cluster is the varicocele streptothricin biosynthesis gene cluster.

The varicose streptothricin biosynthesis gene cluster is about 95kb in length and comprises 41 open reading frames in total, and the 41 open reading frames are analyzed by biological information. The detailed analysis results are shown in Table 1.

Table 1: functional analysis of each gene and encoded protein in the gene cluster for biosynthesis of varicose streptothricin

2. Determination of the boundaries of the biosynthetic gene cluster of varicose streptothricin:

through literature research and interruption of genes on both sides of the genes stvA1 and stvJ, the corresponding mutant strain is detected to find that the mutant strain does not influence the production of the streptothricin; according to the functional analysis of gene coding protein, the biosynthetic gene cluster of the varicose streptothricin is basically determined to be genes stvA to stvJ, covers a region of about 95kb and comprises 41 open reading frames. The biosynthetic gene cluster of the whole varicose streptothricin is 41 genes: 8 genes related to the synthesis of the varicose streptothricin precursor AHBA; 5 genes related to the synthesis of the carbon skeleton extension of the varicose chain hyphal element; 28 genes associated with post-varix streptothricin modification. As shown in legend 2A.

The P450 family genes stvP1, stvP2, stvP3, stvP4 and stvP5 in the gene cluster are subjected to single gene knockout to obtain mutant strains delta stvP1, delta stvP2, delta stvP3, delta stvP4 and delta stvP5, and high performance liquid analysis is carried out on fermentation liquid of the mutant strains to determine that the types of streptothricins produced by the mutant strains and wild type XW-1 (the preservation number is CCTCC NO: M2017417), particularly the types of the streptothricins delta stvP4 and the delta stvP5, and intermediate products 7, 8, 9 and 10 are respectively produced. As shown in legend 2B.

3. Determination of in vivo function of genes involved in the biosynthesis of varicose streptothricin:

each gene is sequentially knocked out on a varicose streptothricin gene cluster, and possible functions of some genes are basically determined by detecting fermentation of corresponding mutant strains and a liquid phase mass spectrum combination instrument (LC-MS) of fermentation products. The genes responsible for biosynthesis of the varicocele streptothricin precursor AHBA, namely 8 genes of stvG, stvH, stvI, stvJ, stvK, stvL, stvM and stvN; the genes responsible for carbon skeleton synthesis of the varicose streptothricin, namely 5 genes of stvA, stvB, stvC, stvD and stvE; genes stvA1, orf1309, orf1308, orf1307, orf1306, orf1305, orf1304, orf1303, orf1302, orf1301, stvO1, stvO2, stvO3, stvP1, stvM1, stvP2, stvA2, stvP3, stvF, stvP4, stvO4, stvP5, stvM2, stvM3, orf1272, stvR1, orf1270, stvR2, which are responsible for post-modification of streptothricin, constitute a total of 28 genes.

4. Biosynthesis of streptothricin

Biosynthesis of streptothricin in streptomyces takes UDP glucose as an initial compound, and an initial extension unit AHBA molecule of the streptothricin biosynthesis is formed under the catalysis of protein expressed by 8 genes related to the AHBA synthesis, as shown in figure 3A. AHBA takes acetate and propionate as extension unit to extend under the catalysis of protein expressed by 5 polyketide synthase genes and finally cyclizes to form a varicose streptothricin mother nucleus structure, and the varicose streptothricin mother nucleus forms varicstreptothricin compounds with different structures under the catalysis of protein expressed by 28 post-modification genes, as shown in figure 3B.

The following further provides examples which are intended to aid in the understanding of the present invention and are intended to be illustrative only and do not limit the scope of the invention.

The experimental procedures, for which specific conditions are not indicated in the following examples, are generally carried out according to conventional conditions or those described in the Streptomyces protocols, or according to the conditions recommended by the manufacturer. Percentages and parts are by weight unless otherwise indicated.

Materials, reagents and the like used in the following examples are commercially available unless otherwise specified.

Example 1 extraction of the Total DNA of Streptomyces aspergillinus strain Streptomyces XW-1 (accession number: CCTCC NO: M2017417):

taking about 1cm on SFM flat plate²The Streptomyces XW-1 (preservation number: CCTCC NO: M2017417) is cultured in 80mL of TSBY medium at 28 ℃ and 220rpm for 48 hours. 80mL of streptomycete bacterial liquid is centrifuged at 5000rpm and 28 ℃ for 10min to remove supernatant, and thalli are collected. Dissolving the thalli in 15mL of set buffer, shaking and uniformly mixing, centrifuging at the speed of 5000rpm and the temperature of 28 ℃ for 10min, removing supernatant, and repeating twice; dissolving the thalli in 10mL of set buffer, shaking and uniformly mixing, adding 50 mu L of lysozyme solution (100mg/mL), and placing in a water bath kettle at 37 ℃ for warm bath for 1 h; then adding 280 mu L of proteinase K solution (50mg/mL), uniformly mixing, adding 600 mu L of 10% SDS, reversely mixing, placing in a warm bath at 55 ℃ for 2h, reversely mixing every 15min in the period until the mycelium is cracked and transparent; then adding 4mL of 5M NaCl, reversing and uniformly mixing, and placing the bacterial liquid at room temperature to about 37 ℃; adding 10mL of chloroform, reversing and uniformly mixing to be milky white, rotating at 5000rpm and 28 ℃ for 10min, taking out supernate, adding 0.6 time volume of isopropanol, and uniformly mixing; after mixing, flocculent DNA is separated out, the separated DNA is carefully selected out and washed twice by 75 percent ethanol, and the mixture is placed in a ventilated place for drying and is dissolved in proper amount of ultrapure water. The size of the DNA was determined by nucleic acid electrophoresis (40v, 12h) and the purity of the total DNA extracted was determined by measuring its concentration and OD value with Nanodrop 2000.

Example 2 fermentation conditions and product High Performance Liquid Chromatography (HPLC) detection conditions of Streptomyces aspergillinus-producing Streptomyces XW-1 (preservation number: CCTCC NO: M2017417)

Coating mycelium of Streptomyces XW-1 (preservation number is CCTCC NO: M2017417) on SFM solid plate, culturing at 28 deg.C for 48h, and taking 1cm size with inoculating loop²The agar block is inoculated in a TSBY liquid culture medium for seed culture, cultured for 48 hours at 28 ℃ and 220rpm, transferred into a fermentation shake flask according to the inoculum concentration of 5 percent, and cultured for 7 days at 28 ℃ and 220rpm in a liquid SFM medium. Collecting fermentation liquid, centrifuging the fermentation liquid at 6000rpm for 20min, and collectingThe supernatant was extracted with ethyl acetate of the same volume and the extraction was repeated twice. The extracted ethyl acetate is concentrated and dried by a rotary evaporator, then is dissolved in 10mL of methanol, and finally is subjected to HPLC and LC-MS detection and analysis by a 0.22 mu m microporous filter membrane.

HPLC detection conditions: the phase A is ultrapure water, and the phase B is acetonitrile. 0-20min, 35-65% B; 20-28min, 65-95% B; 28-30min, 95% B; 30-33min, 95-35% B, 33-38min and 35% B. The flow rate is 1mL/min, the detection wavelength is 245nm, and the column temperature is 30 ℃.

Example 3 method for conjugal transfer of Streptomyces varicocellatus producing strain Streptomyces XW-1 (preservation number: CCTCC NO: M2017417)

The target plasmid to be conjugately transferred is firstly transformed into the competence of Escherichia coli E.coli ET12567/pUZ8002, after a transformant is grown, the positive monoclonal is inoculated into 5mL of LB culture solution, the culture is carried out overnight at 37 ℃, and the bacterial liquid is inoculated into 5mL of LB culture solution according to the proportion of 10% and cultured for 3 hours at 37 ℃. Taking host streptomycete XW-1 (preservation number is CCTCC NO: M2017417) growing on SFM solid plate about 1cm²Inoculating into TSBY liquid culture medium, culturing at 28 deg.C and 220rpm for 48h, centrifuging at 5000rpm for 3min, removing supernatant, washing twice with 2 × TY liquid culture medium, and centrifuging at 5000rpm for 3 min. The cut 1mL of gun head is used for sucking about 100 mu L of surface tender streptomycete thallus, the thallus is uniformly mixed with the same volume of escherichia coli E.coli ET12567/pUZ8002 containing specific plasmids, the mixture is incubated for 30min at 28 ℃, and finally the mixture is spread on ABB 13 solid culture medium. After 12h, 1mL of ultrapure water containing the appropriate amount of antibiotic for screening was added to cover the cells, and the cells were incubated at 28 ℃ for 5-7d until zygote outgrowth.

SEQUENCE LISTING

<110> Wuhan university

<120> varicose streptothricin biosynthesis gene cluster and application thereof

<160>42

<170>PatentIn version 3.3

<210>1

<211>95304

<212>DNA

<213>Streptomyces spectabilis

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gtcaccaacg gcgcccgcct ggagacgtac accatcgagg gcgagcgcgg cagcggcgtc 60

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agctacgccc aggtcgacga cgccgaggcc cgcaccctgc ggccgcgggt cgtgcacgtc 180

gacgagggca accgcgtcgt ggccctcggt gacgacgccg ccgcaccggt gccgggcgcc 240

gccgacatgg cgcgctcccc gcaggcggcg gcgcgcgcct agcggcacca cgcgacagcg 300

gcacggccat acggcgcccg gccggggcgc ggcgaggagc ggcgacgatg agtgacaccg 360

cggcggccgg gcggccgttc ggcatccggg acgacagggc gcgcggcgtc ctgctcgccg 420

aggaggacgg gcagaccgtc ggccggatcg tctacttcac cctggccgcg ccggagcccg 480

ccctcgtgcc cgtgcacacc gaggtgccgc ccgcccacga gggccgcggc atcgcgagcg 540

ccctggccac ggagctgtac gcgctcgcgg cccgcgagga ccgcgcggtc gtgccgctgt 600

gcccgtacgt cgccaagtgg gcggagcgcc accaggtccc ggcgccctcc gccgcgctcg 660

tcgaggccgc gctcgccaag gtcgaggacg acccgtcgac gtggtgaccc tcgcgctcct 720

gcacacctcg cccgtccacg tgccggtctt cgacgcgctc cgggaccagg accagccggg 780

cctggcactg tgccacctcg tgcacgaggg gctgctcgac cgcgcccgcg acgaagggcc 840

cgacgccgtc gcggacgagc tcgggcagat cctggaacgg gccgtggccg acggcgcggg 900

cgccgtgctg tgcacctgct cgacgatcgg cggcgtcgcc gaggcccgcg ccgccgcgct 960

cggcgtgccc gtgctgcgcg tcgaccggcc catggcggcc gccgccgtca ccgagggccc 1020

ggcggtcacg gtcctcgcca cggtcaccag caccctcgcg cccaccgccg ccctcctggc 1080

ggaggaggcc gagcgggccg ggcgcaggat cgacgtgcgc accgtcgtgg tggacggcgc 1140

gtgggagcgc ttcgaggcgg gggaccggga cgggtatctg cggctgatcg ccgccgccgt 1200

cgagcgggcc gacgccgacg gcgccgatgc cgtcgtgctc gcccaggcct cgatggcgga 1260

cgccgccgcc cgctgtgccg tgcgggtgcc ggtcctcgcg agcccgcgca cgggtctgcg 1320

cgccgccgcc ctgtgcgtgc cgcgcggctc ctagcgcgcc gcgcgccccg tcgggaggag 1380

tggactcggc gcgcgtgggc acgcgagccg ggagagagtg ggggacggcg gcctccgcgg 1440

cgccgcgcgc ggccgtcccg ccggaaggtg ccggccgcct ccacccaccc ctgggaggaa 1500

gcatgaccaa cccgttcccc gaccccgtcc agcccggccc cactccgggc ccccgcccgg 1560

agccgccgcc ggggcccccg ccaggaccgg ggcccgcccc gggcccggtg cccgtgccgc 1620

agcccccgcc gtccccggtg cccaagccgc cgggcccgga ccccgtaccg gatccggacc 1680

cgacgcccga gccgatgagc taggcccgct ccgcgcgagg gctacgcctc gacctccgag 1740

cggtcgccgc cccacagcgt gtggaacgaa ccctcgcggt ccgtacggcg gtaggtgtgc 1800

gcgccgaagt agtcccgctg cccctgcgtg agcgcggcgg gcagccgctc cgcgcgcagc 1860

gcgtcgtagt aggcgagggc cgcggcgaag cccggcgcgg gcacgccctg ctgggtcgcg 1920

gcgacgagca cggcgcgcca gtcgtcctgc gccgcgccga tctcgcgcgc gaagccctcg 1980

tcggcgagga ggctcggcag gtcggcgcgc gcgtcgtacg cggcgcggat gcggtcgagg 2040

aacgccgccc ggatgatgca gccgccgcgc cagatcgagg cgaccgcgcc caggtcgatg 2100

tcccagccgt actccgcgct gcccgccgcg atctcgtgga agccctgcgt gtacgacacg 2160

atcttcgagg cgtacagcgc ctgctcgacg cggtcggcga aggccgccgc cgcctccttg 2220

gacagcggtt ccgcgaccgg gcccgccagg tcgcgcgagg cggcgcgcag ctccgtgtgc 2280

cccgagacgg aacgggcgaa caccgcctcc gcgatgcccg acaccggcac gcccaggtca 2340

agggcgatct gcacggtcca gcggccggtg cccttctgct cggcccggtc cgccacgacg 2400

tccacgaacg gcttgccggt cgcggcgtcc acgtgcgcca ggacctcggc ggtgatctcg 2460

atcagatagg agtcgagccg cccccggttc caggtgcgga agatgtcggc gatctcggcg 2520

ggggagtagc ccgccacctc gcggagcagc tggtacgcct cggcgatcag ctgcatgtcc 2580

gcgtactcga tgccgttgtg caccatcttc acgaagtggc ccgcgccgtc cgggccgatg 2640

tgcgccgtgc aggggctgcc gtcggcggcc ttcgcggaga tcttctccag catcggaccg 2700

agcgccgcgt acgactccgc ggagccgccg ggcatgatgc tcgggccgtt gagcgcgccc 2760

tcctcgccgc cggagacgcc ggtgccgacg aagtggatgc cgagctcgcg cagttcgcgc 2820

tcgcggcgcc gggtgtccgc gaagtgcgcg ttgccgccgt ccacgatcat gtcgcccggt 2880

tcgaggagcg gtgcgaactc cttgatgacg gcgtccgtcg gctcgcccgc cttcaccatg 2940

acgacgaggc ggcgcggccg ctcgagggcc gccacgaagt ccttcgcggt ctccgtgtag 3000

acgaactccc cctcgtcgcc gaactcgtcg acgagcgcgc gggccttggc cgcggtgcgg 3060

ttgtgcacgg cgaccgtgaa gccgttgcgg gcgaagttcc gggcgaggtt gcgccccatc 3120

accgcgagac ccgtgacgcc gatctgcgct gaactgctca tgtgctagct cctgggttcc 3180

tggaaatccg acgtaccgcg accgcccggc acaccgcctg acctgccggg cacgtcatgg 3240

gtgacgcatg tgtcgtaggt aacgcgtctg cccgtcagta tcactccgcg gcgtaccgtg 3300

accttggaca ccgtgcccga aggaggagcc gcccgtgccg catttcgacc tgccgctcga 3360

ccaactgcgc cagtaccacc cggacctgcc ggaacccgag gacttcgacg ccttctggga 3420

gaagaccctc gcggccgcgc gggagcacga cctggacgtc cggttcgagc ccgtggacgt 3480

gccgctcgcc tcggtcgacg tcttcgacgt gaccttcgcg ggcttcggcg gtcaccccgt 3540

caagggctgg ctgctgctcc cggccggggc gcgcgagccg ctgcccgccg tggtcgagta 3600

catcgggtac ggcggcgggc gcggcctgcc gcacgagcga ctgctgtggt cggcggccgg 3660

gttcgcgcac ttcatcatgg acacgcgcgg gcagggcagc ggcggctcgg taggcgacac 3720

ccccgacccc gtgggcagcg cgccggcgta ccccggcttc ctgacgcgcg gggtcgagga 3780

cccgcacgag tactactacc gccgcgtctt cacggacgcc gtgcgcgccg tcgaggcggt 3840

gcgcgcgcat ccgctggtgg acgccgcgcg cgtggccgcg gtcggcgaca gccagggcgg 3900

cggcatcacg ctcgccgtcg gcgggctcgt gccggacctc gccgcgatct ccccgaacgt 3960

gccgttcctg tgcgacttcc cgcgcgcggc gacgctcacc gcccggcccc cgtaccggga 4020

gatcggactg tacctggggg cgcgcagggg gcgcgaggac cgggtgttcc gcaccctctc 4080

gtacttcgac ggcgtgcact tcgcggcgcg cggccgggcg cccgcgctgt tctcggccgc 4140

tctggaggac gagacgtgcc cgccgtcgac ggtcttcgcc gccttcaacg cctacgcggg 4200

cagcgagcgg tccatggagg tctaccgctt caacgaccac gagggcgggg gcgtcttcct 4260

ccaggatgtg caactccggt ggcttccggg gcatttgagg aactgaccag ttcaacgccc 4320

tctgcgcgcc ctcctcttgt cacagtgacg gccgaccggc tagcttgcgg ccttgtcctg 4380

tggctgacgt gtgaccttgc gtacaacgcg tgtctgaggg ggctctcatg gccttacgag 4440

gccggcatcg ccggtaccag ccgagccgga tcaaccgtgc ctcgctcacc gtcacggcgg 4500

gcggcgcggg cctcgcgctg cccctggtgt ccgcgggcac gggccacgcg gcggacgtcg 4560

acacctggaa caaggtggcc gcctgcgagt ccacgaacaa ctggagcatc aacaccggca 4620

acggcttcta cggcggactg cagttcaccc agtccacctg ggaggcctac ggcggcacgg 4680

cgtacgcgcc gcgcgccgac ctcgccacca aggaccagca gatcgccgtc gccgagaagg 4740

tcctcgacgg gcagggcccc ggcgcctggc ccgtgtgctc ggtgcgggcg ggcctgaccc 4800

ggggcggcga caccccggac atcaagcccg ccacggcacc ccagaagcgc acgggcacgg 4860

cgaacgacgc cagggcggag ggcggccgca cggcaccgag gccgccgcag cgcaccgtca 4920

aggacgtcaa gccggagtcc accccgcagt ccgccgcggg caccgcccgc atgtacacgg 4980

tggtgcgcgg cgacaccctc tccggcatcg ccgacgagcg ccgggtgcgg ggcggctggc 5040

cgcagctgta ccagaacaac cgccgtgcca tcggcgacga tccggacctc atcaccccgg 5100

gccagcggct ctcgctgcgc ggcgcggacg gcaaggagaa gcggcccgcc cggccgcccg 5160

agcgcaggaa gccggagggc acggagcgcc cggacaagcg gccggtgcgc gcgcccgaga 5220

agcagcgctc gcatccgcag gccaagccgc aggagcgcaa gcaggccaag ccgcaggagc 5280

ggaagcaggc caggcccgcc ccgaagcgcg acacggcacc ggcgggacag cggaaggcga 5340

aaccggccgc acaggcgcag tcgggcgcca ccgcgcccgt cgccgcctcg atcggcacgc 5400

cctaccgcgc ctccggcagc tcctggtcga agggctacca cacgggcgtc gacttccccg 5460

tgcccacggg caccgcggtg aaggccgtcg ccgcgggcac ggtcgtcgag tccggctggg 5520

gcggctcgta cggctaccag gtcgtgatcc gccacgccga cggcaggtac tcgcagtacg 5580

gccacatgtc ggcgctctcg gtgaaggccg ggcagaaggt cgtcccgggc cagcgcatcg 5640

gccgctccgg atcgaccggc aacagctcgg gcccgcatct gcacttcgag atccgcacgg 5700

gccccggctt cggctcggac gtcgacccgc tggcctatct gcgggcgcgc ggcgtccgca 5760

tctgaggcgc ccgcgccgcg gacacacgcg tacgggcccc gggtctcggt gagagacccg 5820

gggcccgtac gtacgcgtgc cgtccgtctt ccgctacgcg cgtgccgccc tggcgtccgc 5880

acgcgggaac gtctgcagga ccggttgcat cggcacgccc gcgagcccgg cgaggccctc 5940

gggcaggaac gactgctccg gcgcggcctg ttcgcccggc cgcgaccgcg tgcccgcgtc 6000

ggcgccgggg cgctcgtcgt ggtccggcac gcgctgctcg gtgaccgtgg ccgtctcctc 6060

cgacgcggcg gaggcggggg agacggcagc accgggagag acaggggaga tgggggagac 6120

gggagaggtg ggtgaggcgg tcgagggcgt ggggacggag cgggccaccg gctcggcggc 6180

gggccggtcg tcgcggggcg cggccggtgc ggcgtcgtcc cgggccgcgg ccgaggccgc 6240

ctccgccccg atgcgctccg tggtcagcag gataagcccg cccgcggcga ccacgccgca 6300

ggtcagcgcc accgccgtgc ccacggctcc gtagcggaag gattctccga acagggtgag 6360

gccgaccgcg gccgctatca ccgggttcac caccgtcacc gtggacagcg gcgccgcgag 6420

gcccgcgccc cggtaggagg cctgggacag caggagtccg gcgaccgcga gcaccgcgat 6480

caccgcgagg ctcggcacct gccgggtcag cggctcgtgg gcgtcccagt ccaccgcgac 6540

cgtcttcgtg aagaccgagg agatgccgaa ggcgatgccc gccgccgtgg cgagcagcac 6600

gctgcgcacc gccgggtgcc ggtgcgccgc gtgcgcgagc agcatcaggc agaccacggc 6660

cccgcccgtg ccgagcgcga gcatgacgcg ctcggtgtcg gccagggacc gggagtcggc 6720

ggcctgcgtc agcgagagca ggcccgccag gcccagggtg gccatgaggg cgccgcgcca 6780

ggcggtggca ccggccttgc ggcgcacgaa catcgccgcc atcggcaggg cgaacacgat 6840

cgtcagggcg cccaggggct gcaccaggct cagcggcccg taggcgagcg ccacgacgtg 6900

cagcagcccg ccgaggccgt tcaggaccac cgcgacccac cacacgccgc ggcgcagcgg 6960

cgcgtactgg tactgcgagg ccgtcgccgc cacctgctcc tggaggatcg ccccgcccgc 7020

gtacgccacg gcggagacga gggagagcag gacggacagc gcgagggcgc tcatgaggcg 7080

ctccaccggt gagggcaacg ccggtcggcc cggtacgtgt cgtgcccgta cgtccggtac 7140

gaacggtgcg gcgtgcggtc ggcttccatg ttcaccacaa tgtcgcgcga agcgcggcaa 7200

gtcgtcgtcc ctgagcagtc aatccgtcct actaccgatg gagtacgacc tcccgtatgt 7260

cctccccagg tctggttacc cacgaggtcg ggggcttctg atgtactgct gcggtgacag 7320

ctcgcagccc tgacatcgcc tgggacgtgg tcggatccgt cggcggcttc ttcgccctgc 7380

ggacggggga gccgccgggt cggacactcc ccatggtcgc acgcgtgtac gagatcggca 7440

gggtggtcac ggagggtgac ccgctgtcct tccgcgtcac caaggtcgcc gcccggctcg 7500

ccgctcccga gccgcgcgtc gcggcctcca tcgcccacct cggcctcgcc gcccgcctgt 7560

ggtccctcgc gctcggcccg gccgccctgt acgggcgcgt gcccgacctc gacccgcggc 7620

ggctgcgctg ggacgccgac ggcagctcgc cggacgacct gtggctcagc ggcgaccgca 7680

ccttccccgc cgacgccgcg cgcatccggg aagtcgtcct ggaaggtcac ctcgcgccgc 7740

tcgcggccgc cctgcgcgcc cgctaccgga tctcccccgc cctcctgcgc ggcaacgcgg 7800

gctcggccct cgcgggcgcc gcccgcgaac tgcacgcctg ggcggagcgc accggccgcc 7860

ccgaggcggg acggcgggca ctcgccctcg ccaccgaact cttcaccgcg ccggagctgc 7920

gcggcaccgg caccctggac ggcaccgcgt tccggcggcg cagctgctgc ctgtactacc 7980

gctgtccgaa cggcggcctg tgcggcgact gttgcttcga ccggccgccg cagcggtctt 8040

ccctcgaagc gcccgctggg tgaccatgag ggggccggcc gacgacgggg ggctccgctc 8100

gtgaaagtgg gactgctcac ccgcgagtac ccgccggacg tctacggcgg cgcgggcgtc 8160

cacgtcgagt tcctcgcccg cgagctgcgc gccctgaccg acctcgacgt ccactgctgg 8220

ggcaccgaca ccgaccgcgt ggccgccgcc gacgcggcgg tgcgccaccg cgcgtggagc 8280

gccctcgaca ccgccaatga cgcgctgcgc accctgtccg tcgacctcgc catggcggcc 8340

gcgctcgacg gccgcgacct cgtgcacacc cacacctggt acgccaacct cgccggccac 8400

gtcggcaagc ttctgcacgg catcccgcac gtcatgacct cgcactccct ggagccgctg 8460

cgcccctgga aggccgagca actgggcggc ggctacgccg tgtcgagctg ggccgagcgc 8520

accgccgtcg aggccgccgacgccgtcatc gccgtctccg gcgccatgcg cgaggacatc 8580

ctcgcctgct atccggccgt cgaccccgcg aaggtgcgcg tcgtccacaa cggcatcgac 8640

accgagctgt accgcccgga cccgggcacc agcgccctcg tccggcacgg cgtcgacccc 8700

acccgcccgt acgtgctgtt cgtcggccgc atcacccggc agaagggcgt gccccacctg 8760

ctgcgcgcgg cccggtgcct ggagcccggc gtccagctgg tgctgtgcgc gggcgcgccc 8820

gacaccccgg agatcgaccg ggagttccgc gccctgttcg aagagctgag ccgcgcccgc 8880

gccggcgtcc actggatccc gcgcatgctg ccgcgccccg aggtcgtgca actcctcagc 8940

cacgcggcgg tgttcgcgtg cccctcggtg tacgagccgc tcggcatcgt caatctggag 9000

gccatggcct gcggcacggc cgtcgtcgcc tcgcgcgtcg gcggcatccc ggaggtcgtg 9060

gcggacggca cgaccggact cctcgtgccc tacgaccggc acgacgagga gcgcttcgag 9120

cacgacctcg gtctgctcct gaaccacctg gccgccgatc cgcgccgggc ggcggccatg 9180

ggcgcggccg gacgctaccg ggccaggcgc gagttcggct ggggcacggt ggcccggcgc 9240

acggtcgagg tgtacgagga cgtactgggc gtgctcaagg caacgtaagc gggcacacgg 9300

acactcgggg gcgtccgcac gcccccgcca cccggccgag gaacggtccg ggcccacagg 9360

gcggtagagg gggcggcgat gcgcggcgga ccttcggtgc tggggatcgt cctggcgggc 9420

ggcgcgggca agcggctgct gccgctcacc gccgaccgcg cgaaaccggc ggtgaccttc 9480

ggcggcacct accgcctcgt cgacttcgtg ctctccaacc tcgtcaacgg cgacgtcctg 9540

cgcatctgcg tcctcacgca gtacaagtcg cactccctgg accggcacgt gtccaccacc 9600

tggcgcatgt cgagcctgct cggcaactac gtcacgccgg tgcccgccca gcagcgcctc 9660

gggccgcgct ggtacctggg cagcgcggac gcgatcctgc agtccctgaa cctcgtccac 9720

gacgaacagc cccactacgt cgcggtgttc ggcgccgacc acgtgtaccg catggacccc 9780

cggcagatgc tggagcagca cgtcgagtcc ggcgcgggcg tcaccgtcgc cgggatccgc 9840

gtgccgcgcg cggacgcccg gcagttcggc gtcatcaccc cggcgcgcga cggcacgctc 9900

gtggagcgct tcctggagaa gccgcaggac ccgccggggc tgccgggcgc cccgcaccag 9960

gtgtacgcct ccatgggcaa ctacatcttc accaccaagg ccctcgtgga cgccctgcgg 10020

caggacgccg atgacgagga ctcggcgcac gacatgggcg gctccatcct gccgatgctc 10080

accgagcgcg gcctggccca ggtgtacgac ttcgacggca accacgtgcc gggggagacc 10140

gcacgggacc acggctactg gcgcgacgtc ggcacgctcg acgcgtacta cgaggcgcac 10200

atggacctga tctccgacca gcccgtgttc aacctcgaca accgctgctg gcccatctac 10260

acccaccccg gccagctccc gcccgccaag ttctgcgcgg gcggcatcgc cggggagtcg 10320

atcgtcagtc ccggctgcgt catccgcggc caggtcacgc ggtccgtgct ctcgcccggc 10380

gtcaccgtcg aggagggcgc ggtcgtccag ggttcggtcc tccacgacaa cgtgcgcgtg 10440

ggccggggcg cggtggtgcg cggcgccgtc ctggacaaga acgtggacgt cccgcccggc 10500

gccacgatcg gcgtgaaccc ggagcgggac gccgagctgt acacggtctc gcgggccggg 10560

gtcatcgccc tcggcaaggg gcagtgcgta ccggagtgac cggagcggcc ggggcgacca 10620

gagcggtcgg ggcggccggg gcgcggggac ggcccggcgg agactaccca cgctggtgat 10680

ggttccggcg ggcggtgagg gcgagactga gcgcacttca gacccgcagt gcgtcggcag 10740

ccgtgccggg agcgacgtcg ccggcctgct cgccacggca gcactccact cgaaggacct 10800

cacttgacgg aaacaagagc tggggattcc gcgcggccgc tggaggtggc cctgatcggc 10860

gccggactga tcgcgcggct gcacctcgac gcctggatca gcacgggggc gtccgtgcgc 10920

gtccactcca aggacggccg cgcccccgac ctggcacgcg cctgcggcgc gcgggccgtc 10980

gacaccctcg acgaggcgct ggacggcgcg gacgtcgtgg acatctgctc cccgacgagc 11040

agccaccacg agatcgccat ggccgccgtc gcggcgggcg tcggcatcgt gtgcgagaag 11100

ccgctggccg ccgacgtgac ggaggccgag gagatcgtca ccgcggcgga gcgggccggg 11160

gtgcggctct gcccgaccca caacgcccgc ttcgccccgg cgttcgcccg gctccacgac 11220

ttggtcgcgg cgggcggaat cggcgcgggc gcggtcgcca cgttcgacgt cgccggatac 11280

cacccgcggc cctggaccgg ccgcgcctcc gcgcggtcgg gaggcatcct gaccgaccag 11340

atgctgcacg gcgtcgacat cgcctactgg atcttcgggg acgtcgtccg cgtgcacgcc 11400

cactaccagg ggcagctcac cgccccggcc cccgagggcg ccttcgcggt cggcaccgcc 11460

gtcctcaccc acgccgacgg cgcgctcagc cggatcgtga gccgctggac ggcgacgccg 11520

gacccgccca tccgcgtcgc ctaccacgtc tcgggcacgg gcggcacggt gctccacgac 11580

tccgaatggc cccaggagat caaggtgttc ggcggcgcgg gcggggcctt cgccccgcac 11640

ggggaatcgc cgttcctcgc cgagatccgc gagttctccc gggcgttcca gggcggccca 11700

gagccccgcc tcggcccccg cgacgcgctg gccgccgtcc gcatcaccca ggccgccgcc 11760

gagtccgcga gcacgggccg ggccgtggaa ctgcccgtga agggggccgc atgaaggtcg 11820

cagtgctctc gctcgcccac gaacgcgccg cggcgtacgc ccggctgctg cgcgccatgc 11880

caggcgtcga cctgctcgtc gcggaccccg aaggagcgcc ggacgagcag ggccgggggc 11940

aggccgcggc ccggcggctc gacgcggcgt acgcgggcag ttgggacaag gcgttcgcgc 12000

tgcgccccga cgccgtcgtc gtcacgagcg aggtcgcccg ccgccgtgaa ctggtcgagc 12060

gcgccgccga gatcggcgcc cacgtcctgt gcgagcggcc gcaggcggac acggaggcgg 12120

acgccgatgc catggtgcgc gcctgcgagg acgccggggt gcggctgacc ctcgcggcac 12180

cggcctgctt cagcccggcg ctcgccgccg tgcgcgcaca gctcgccgac ggcgcggcca 12240

tcggcccgct caccaccgtc cacggcacgt actacggccc cggcgaccgg gcggccggcg 12300

gagcgctgcg cacccacgcg cccgtcctgc tcgacctggt ggacaccgtg ctcgacggcg 12360

cacccgcggc gcaggtctac gcccagtcga acagcgtcct cagcggcgac ccggacgtcg 12420

agagcgcggc cctgctcacc ctgcgcttcg ccgacgggac ggtcgcggcc ctcgactgcg 12480

gctggagccc gccggggaac caccgagcag ccgcgggacc caccctgacc ttcgtcggcg 12540

agcggggaag cgtcgagttc accgctcgcc cgaagctgct cggcggattc gactccggca 12600

cggcccgcga gcggtggacc acggccgtga gcgacccgta cgcggtcgtg ctcgacgcct 12660

tcgtcgcctc ggtcgcaacc ggccgcggga ccgggcccga cggcgcgacc ggcgtgcgta 12720

ccctccgggt gatcagggca gcctacgagt cggcacgcac cggtgagccc gttgacgtgg 12780

ccgtaccgca gcccgtacgg ccgtagcgga cccgctcggt ccatcctcat cggaggggtg 12840

cgcggcgcac gcgcccgcac ccctccgccg cgacagggag agacgcgcat gacaaggatc 12900

gggatcatcc tcggcaccac ccgccccggc cgcgtcggac cccaggtcgc ccagtgggtc 12960

gagaaggcag cccggacgcg cggcgacgcg gacttcgaac tcgtcgacct cgccgacttc 13020

gacctgccgc tcttcgacga gccgcgctcc ccgcgcatgg gcgcctatga gcacgcccac 13080

acccgcgcct ggagcgccaa gatcgccgaa ctcgacggct tcgtgttcgt cacccccgag 13140

tacaactggt cgatcccggc cgcgctcaag aacgccatcg acttcgtcta caacgagtgg 13200

aacaacaaag cggcggcctt cgtcggctac ggagcaagcg ccaacggcgt ccgggccgtc 13260

gagcacctgc gcgccatcgc cgcagcggtc cagctctcgc ccgtgtacac acaggtcaac 13320

ctgtcgctcc tgaccgactt cgagcagtac gagaccttca cccccgccga gcgccacgac 13380

gccgcgctcc agcggatgct cagcgaactg atcacccgga cccatacgct cgcgcccctt 13440

cgccgggcgt gaaccaccgc acgtgcaagg aggaccaccg atgagcctgc cgaccgcggc 13500

tcccgccacc gggcccacgc cgccggacga caccaccccc tcggtctacc gattgccttt 13560

tccgcccggg ttcaccgagc gcagcggctg ccccttcgac ccgcccccca tgctccgcga 13620

ccaccacgac cagggcgagg tgcgcgagat cgccctgacc gacggcggca ccggctggct 13680

cgtcaccggg tacgaggagg gccgcacgct gctcagcgac ccccggctca gctccgaccg 13740

gctgcgcaac ccgctggtcg tgaagctgcc ggcggagctg cgcgcccagc tcatggccga 13800

cgaggcggtc gcgggcaact tcatcgccat ggacccgccc gaccacaccc gctaccgcaa 13860

gatgctgacc cggcagttca cgctgcgccg ggtgcggcag ctggagccgc ggatcctgga 13920

gatcgtcacc gagcgcatcg acgccatgct cgcccgcggc aacagcgccg acctcgtcaa 13980

ggacttcggc ctcgcggtgc cgtccctggt gatctgcgaa ctgctcgggg tggcctacga 14040

ggagcgcggg gagttccagg cccgcaccga ggaactgctc aacctggaca tcccgttcga 14100

gaagcagctg gccgccctcg acgagctgcg gaagttcatc ctcggccagg tgcggcgcaa 14160

gcgcgcgacc cccaccgacg acatgctgtc cgacctcatc cgctccgaca ccgaaccgcc 14220

gctcaccgac gaggaagtcg tcggcatggc caacctgctg ctcgtcggcg gccacgagac 14280

gaccgcgaac atgctgtccc tcggcacctt cgccctcctg gagcaccccg agcagttcag 14340

ggccctgcgc gaccgccccg aactcatgga cggggcggtc gaggagctgc tgcgctatct 14400

gtccatcgtc caccacggcc tgctgcgcac caccaccgag gacgtcgaga tcgcggggcg 14460

gaccattccc gcggagtccc tggtcatcat ctcggtgtcc gaggcgaacc gggatccgcg 14520

ccactacgac agcccggccg ccctcgacgt gagccgcccg cgcaggaccc acctcgcctt 14580

cgggcacggc atccaccagt gcatcggcca gcagctcgcc cgcagcgaga tgaccatcgc 14640

cttccccgaa ctgctgcggc ggctgcccgg cctccgcctc gccgtcccgg ccgacgaggt 14700

gccgctgcgc gagaagatgg ccgtgtacgg cctgcacagc ctgccggtga cctgggacgc 14760

gcccgacgcg taggcgtctc gcacgcgtcg tcctaccgcg tacggctctc ccgtgcaccg 14820

ctctcccgta cgccctcccc tccgtacgcc cttccccgcc ctcccgcacg gaatagggcg 14880

tacggaaatg ccgtacaaac acgcgccgtg caaaagcgga attccgcggc ggccgcgcgc 14940

ggccggaatt ccggtgaatt ccggagcgcc ggggaaaatt accaggttga ggggtgctcc 15000

taggggccgc cgctgcctcaccctgggaga cgtcaccagc caatcgtcga cgacagggac 15060

ggaccgggat gaccgacgac aacgcatacc agcacttcga cgcgaccacc ctggactacg 15120

aacgcgcgta tcggaccagc tccctggtgg agggcgtggt gctggagcgg atgccctggg 15180

acatcggcgg accgcagccg gtcctggtcg aactggaaag ggcgggccgg gtgtccggcg 15240

acgtcctcga catcggcagc ggcgtgggtg acaacgcgat ccacctggcc cggtgcggct 15300

atcgcgtgac cggcctcgac gtcgcgccca ccgccgtgga gaaggcgcgc gcccgggccg 15360

cggagaaggg cgccgacgtc accttcgccg tcggcaacgc gacgagcctg gacggctacg 15420

agaaccagtt cgacacggtg accagcagcc tcgtcttcca ctgcttcggg ccgaagcagc 15480

gccgcgcgta cgcggacgcc atcgcacggg tcctgcggcc cggcgggcgg ctgctccagt 15540

ggtgctcccg cggcgccgct gccggtcccg agccgatcac cgaggagacc atcagggacg 15600

ccttcagcgg ccccggctgg tccatcacca cgctccgcgc gcagcacctg acgacgaccg 15660

tcctggacga gcccctgcgc aaggactacg agggagggca gctcgacacc gacgacagcg 15720

gggccacgct gctgccgatc tggctcctcg aggcggcgcg cgactgagcg cccccggacc 15780

cgcgcacgag ggtgccgaca tgaccgagac gcccgccacg ccgacgggcc tgcccaccgc 15840

ccgcgcggcc ggctgcccgt tcgacccgcc ccccggcctc gccgcgctgc gcgaccgggc 15900

cccgctgacc cgcatggagt tcccgaacgg ccacgtcggc tggctggcca cgggccatgc 15960

ggtggtgcgt gccgtgctcg ccgacccgcg cttcagccac cgcaacgaca ggaggcactg 16020

gcccctggcc gacatcggcc gggggttccc gccgctgccc ggcgacatgc tccacatcga 16080

cccgccggac cacacgcgct accggaagct gctcgccggc aagttcacca tgcgccggat 16140

gcgccggctc accggcagcg cccaggagat cgtcgcgggc aagctggacg ccatggagcg 16200

gcacggcggc ccgctggacc tgctggagtt cttcgcccgc cccgtgccga cgctgatggt 16260

ctgcgccctg ctcggcgtgc cgctccagga ccgcgccacc ttccacccgc cggtccgcgg 16320

gaccgacgac gccgcagccg tggaggcgga cgtcgccgcg tacgtcgaga tgacgtacgc 16380

cgacatgcag gagtacttcc ggaagctggt ggcggccaag cgtgccgcgc ccgccgacga 16440

cctgctgagc gatctgacca cgtccgacct caccgaggac gaactggtgg gcctgtgcgc 16500

cgtcatgatg cacgcgggcg tcgactcgac ctcgaacatg ctggccctgg gcacgtgggc 16560

gctcctggag cgcccggacc aactggcggc cctgcgcgag cggccggacc tcgccgaccg 16620

tgccgtcgag gagctgatgc gctacatgag cgtcgtgcac accggctccc gcgcggcgct 16680

ggaggacgtc gaactggccg gggaggtcgt cagggccggt gagtcggtgg cgttctcggt 16740

gcaggccgcc aaccgcgacc cggcccgctt cgccgacccg gacacgctcg acatccggcg 16800

cggtgccgtg gggcacctgg ggttcgggta cggcgtgcac cagtgcctgg ggatgcagtt 16860

ggcgcgcgtc gagatgcgcg tggccttccc ggccctgttc gcgcggttcc ccgccctgcg 16920

cctcgcggtg cccgccgggg acgtaccgat gcgggacgac ctggtcatcc cttacggggt 16980

gcaccggctg ccggtcacct ggtaggtgac gggccgccgc gtgacgctgc cggagcccct 17040

ggcatcgggg ctccggcagc gtcgtgttcg tgtgtgtggt ggtgtgtggg gggcgtgggt 17100

ggtggtcagg cctgcgacgt caggcggttc agcacctgcc gcaggcagtc cagacgcctc 17160

ggcagctccg cgtccccctc gggccacgcc aggtcgatcc ctatgcggcg gtcgaaggtg 17220

gtggccacat agccgccgat ggccgcgaac tcgccggggg ccaccttcct gcgcgaggag 17280

gagtggaagt tggtgaggag caggccgtcc ggcgtccgca gggtcggcac gacgccccag 17340

ttcgacacgc tcaccacggc gggagccggg cccgcggcgc gcggcttcgc gtgcggcgcg 17400

ggctgggtga ccatgtccag gaggctgcgc tggatggagc cgtcggcgag gcccgcccgc 17460

agccgctcgc cgagcgccct gccgatggcg acggcgctgg gttcgacgtc gtcggcggcc 17520

ttgaacaggc cgcccccgac gacgttcgtc ccctcggtcg ggccgatcgg gggagtgagg 17580

cggttgcgca ggttcaccgt gtaccggaac agcagctcgg acaccggcag gccccggacc 17640

tcggcctcga cgaggaggac ggcgcccgag aggaggccgt tgagcgtcac ctgctcgcgg 17700

tggccgagct ccgcgagcgc cgtcgtctga tcctcgctca ggcggtgctg caccacgtgc 17760

cggactatcg ggatccgtgc ggaggcgggc agttcggtca cggtcttcaa cggcgccgac 17820

gcgccgcctt cgccggacgc ggcccggtgg atgccgcgct cggcgagaag gtcctcgagg 17880

gaccgcgggt acggccggcg cggcaggtcg acggacgcgc cccggaccac gtcggcgtag 17940

caggcccaca gggccgagag cagctcgatg gagtggtggg cgtcggcgat gctgtgatgg 18000

gtcagcaggg tcacgctcgc ctcgctgccg tcgcgcacca cgttgagcgc gctcagcatc 18060

cggtactcgt ccaactccac gccggtcagc gggtggtcgg ggtcgcccgc gcagatctgg 18120

acctcgggcg gggcgccgga ctccacgaag gcgagcccgc cgtcgtcggc cacgagccgg 18180

gcggcgagct gcggatgcgc gcggcacacc gccgcgtagg cggtccgcag cgcagccgga 18240

tccagtcggccgacggtacg caccgtgtag cccacgtaca tctccacgtc ggcatagatt 18300

ttctcggtgg gtgtcagcgc acgctgaatt gcagctgtcg tcatgaatga atggcccttc 18360

cgccgacgcc cggcatgtcc gaagcctgat catttttgcc cacggggtac ggcggggcca 18420

tcaccaacgt gagtagtctc cgtaaggccg cggcccgtcc cgctcgtcga cgggcgtccc 18480

gccgcccccg cactccccgg ccgccggaac cgtttcccca cccctatgac caacgggcgc 18540

tagccgtcgc cgacttggcc accccggccc taccaggttg ggggattccg cgccgacacc 18600

gctcgactag cctggccaca gagattcaag cagcatgcgc gcggtgcgca tcccgtgtct 18660

ccggaattcc gctgcttggt ccatgacccc gtgttcgcgc ccttggccga cgttttcgcg 18720

agccgcagcg catgcgggac tttaccccga gcgggaggat ttccatgagc gacgcgattt 18780

cctatgaggt gccgtgggcg cggacccatc agttcgatcc tcccgccgtg ttcgacgcgc 18840

tgcgtgccga gcggccgctg gcgcgcatga cctacccgga cgggcacgag ggctggatcg 18900

ccaccggcca cgaagtggtg cgcgaggtcc tcggcgaccc gaggttcagc cacagcacgg 18960

agatcgggca cttcccagtg acccaccaag ggcagctggt gccgaaccat ccgaagatcc 19020

ccgggatgtt catccacatg gacccgcccg accacacgcg ctaccgacgg ctgctgaccg 19080

gggagttcac cgtccgcagg gcgagccgcc tgaaggcgcg cgtggaggcc gtggccgccg 19140

agcagatcga cgtgatgcgc gcgcacgggt cgcccgccga cctggtggag accttcgcca 19200

ggccgctcgt cctgagggtg ctctccgaac tggtcgggct gccctacgac gagcgcgagc 19260

actacgagca cgcgccgacg ctcctgcacg acccggacgc gaatccgcag gaagtggccg 19320

ccgctttcga gaaggcgggt gccttcttcg tcgaagtcat cgagcgcaag cggaagaatc 19380

ccggggacga cctcctcagc cgcctcacag cggacggtca gctgaccacg gaagagctgt 19440

gcaacatcgt cgtcctgctg ttgttcgcgg ggtacgagac cacggagagc gcgctgtccg 19500

tcggcgtctt cgcgctcctc caccacgcgg aacaactggc ggtgctccgc gcggatccgg 19560

agaaactcga caccgcggtc gaggaactcc tgcgctatct caccgtcaac cagtaccaca 19620

cctaccggac cgccctcgaa gacatagagc tgaacggtga gacggtcaag aagggcgaca 19680

ccgtgaccgt ttcgctgccc gcggccaacc gcgaccccgc gaaattcacc tgccccgaaa 19740

aactggacat cggccgcgac accgccgggc acgtggcgtt cggctacgga atccaccagt 19800

gtctgggcca gaacctggcc cgcgtcgaac tgcgcgccgg aatcgcggct ctcctcgggg 19860

catttcccga cctgcgcctc gccgtcgcca cggacgaggt gccgctccgg ctcaagggct 19920

ccgtcttcgc cgtgaagggc ctgcccgtct cctggtgagt ccccttctcc ctttctccct 19980

ccgaaaggaa ccgcggcatc gtgcgcaccg acctcatcaa gccgctgccc gtcgccctcc 20040

tggagaacgc ggcccgcttc cccgacaagg tggctttcga ggacgaccgg cgggcggtca 20100

cctacgcaga ccttgaggcg cggacgcgca ggctcgccgg tcacctcgcg gacctgggcg 20160

tggcccgcgg cgaccgggtg gccatctgcc tcggcaacac cgtctccacg gtggagagtt 20220

acctcgcggt cgtccgcgcc ggcggcatcg gcgtgccggt caatccggcc tcggcccttg 20280

ccgaactgga gtacgtactg gccgacagcg gcgccacggc ggtcatgacc gacgccgcgc 20340

aggcggcgcg cctggggcgc tcggccgccg tggaccccgc caccacgctg ctggtgaccg 20400

gcgaggcccc cgaggtgccg ggagcgcacg cgtacgacac actcgccgtc accgagccgc 20460

ccgtgcccgc ccgggacgac ctcggcctcg acgacgtggc ctggatgttc tacacctccg 20520

ggacgaccgg caggcccaag ggcgtcctgt cgacgcagcg caactgcctg tggtccgtgg 20580

cctcctgcta cgtgccgata ccgggcctca cggaccggga ccgggtgctc tggccgctcc 20640

cgctgttcca cagcctctcc cacatcgcgt gcgtcctgtc cgtcaccgtg gtcggcgcca 20700

ccgcccggat catggacggc agctccgcgg acgacgtgat cgaggcgctc cgcgagggcg 20760

gcgcgacgtt tctcgccggg gtgcccacca cgtaccacca cctggtgacg gccgcccggc 20820

agaacggctt ctccgcgccc gccctgcgga tcggcctcgt cggcggcgcg gtcaccgggc 20880

ccggcctgcg ccgggacttc gaggacacct tcggcgtgcc gctggtcgac gcctacggct 20940

ccaccgagac ctgcggggcc atcaccatca acccgcccga cggcgcccgc gtcgacggct 21000

cctgcggcct gcccgtgccg ggcgtcggcg tccgcgtcgt cgaccccggc accggcctcg 21060

acgtccccgc cgggcaggag ggcgaggtgt gggtcagcgg gccgaacgtc atggtcggct 21120

accacaacag ccccgaggcc accgccgagg cactgcgcga cggctggtac cggaccggcg 21180

acctggcccg gcgcgacgac gcgggctact tcaccatccg cggccggatc aaggaactcg 21240

tcatccgggg cggcgagaac atccaccccg aggagatcga ggcggtcctg cgcaccgtcg 21300

agggcgtcgc cgacgcggcc gtcgcgggcg caccgcacgc gacgctcggg gaggtgcccg 21360

tcgcgtacgt ggtccccgga cccgccggct tcgacaccgc gacgctgctc gaccggtgcc 21420

gcgagcggct ctccgcgtac aaggtgccgg agcggatcca cgaggtcacc ggcatcccgc 21480

ggaccgcgtc gggcaagacg cggcggcgcc tccttgccga ccggccgagc agactgcggt 21540

acgcggcgag cggccaccac gaggggctgc tgagcctcga ctgggcctcg gtcgcggccc 21600

cggcggaccc cggccccccg gcccgggact gggcggtggt cggcccggcg gcggccggac 21660

tcggcacggc cctgaaggcc gcgggcgccg acgtacggca ctacccggac ctggcggcgg 21720

tccgggccgc ggcggccgac gaggacgcgg ctcccgaggt gacggtcgtc gtacggccgc 21780

cggtaacggg cgtcgtacgg ccgtcggccc cggcggcacc ggggggcgcg cgggaggcga 21840

tcgccgccgg ccgtgagggc gcgcggctgt gggccgacga gctgcggtcc tggctggaac 21900

gtccggagtc ggccgcgtcc cgcctcgtgg tgctcacccg cgccgcggtc gtggccagcg 21960

cgcaggacga tcggcaggac ctggccgcag ctccgctgtg ggccgtcgtc ccgtccttcc 22020

aggagggggg tgcggggtcg ctcaccctcc tggacctgga tgccgacgcg gacccggaca 22080

tcttgtgcgc cgcgctcgcg acaggcgagc cgcggatcgc ggtgcgtgcg ggcgccctcc 22140

tcgcgccgcg accgagtcga ctgccggtgg ccgacgggcc gggggcggac gggcggggga 22200

cggacgggcc gggggcggag gcctggcccg gagccaccgg cacggtggtg gtcaccgggg 22260

cggacacgga gcacggcgcg gcactcgcgc accggctcgt cacggtccac caggtggaga 22320

gcctgctcct cgtcgacacc ccggggagcg ccggtacgcc gaccgcgccc gctcccccgg 22380

gggcggacat cgtccgggtg gccgccgacg ggcagcaccc ggagcagctg cgggccgcgc 22440

tcgccgacct gccccgtccg gtgagcgcgg tggtgcacgc cgccgacgac cccgaactgg 22500

ccctgcttct cgacgagatg accgcggcga acggactcgc cgccttcgtc gccgtgtccc 22560

atacggccgc gccgctcggc acgcccgacc gcctcggggc ggccgtcgac ggagccctga 22620

ccgaggcggt ggtgcgtgac cggcggctgc ggaacctgcc cggcacgttc ctcgcccggg 22680

ccgacggggt cgacatccgg gcaggagggg ccgacacggc gtgggcgggc agcccgctcc 22740

tctccgcgtt cgacctcgcc ctgaccacga acccgccgac gctcctggcc ctcgacccga 22800

gcgcgctcgc cgacagctct cgactcccca ccctgctcgc gaccttgacg gaaccgcagc 22860

cccggcggag cgagcccgac gacgccgtcg cggcggccct gcgcgagcgc ctcgccgcgc 22920

tggacgagcg tgcgcagcgg gcgctcctag aagacctggt ccgtacgcgg gccgccgcgg 22980

tgctgcaccg gtcagggtcc gaaccgatac cgtccgaccg ggccttccgc gacctgggct 23040

tcacctcgat ggccgtcgtc gaactgcgca accggctgac agagagcacg ggcctgccgc 23100

tgcccaccac cgtcgtcttc gaccacccca cccccgaggc cctcgcggcc cggctgcgcg 23160

ccgagatcct cggggtcccg cagccggtga ccgagccggt cccggcggcc gaccccggcg 23220

aaccgctcgc catcgtgggg atggcgtgcc ggttgcccgg cggcgtggcg ggcccagagg 23280

atttgtggca gctcgtggcc gagggccggg acgcggtgtc gggcttcccc gaggaccgcg 23340

gctgggacct ggagggcctg ttcgacccgg acccggaccg cgcgggcacc tcgtacaccg 23400

accagggcgg cttcctgcgc ggggcgggcc tcttcgacgc cgggttcttc ggcatctcgc 23460

cgcgcgaggc cctggcgatg gacccgcagc agcggctgct cctggaaacc tcatgggagg 23520

ccctggagcg ggcgggcgtc gaccccctgt cgctgaaggg ctccgacgtc ggggtgttct 23580

ccggcgtgtt cggccagggc tacggggcga ccggcaccgg cgtcgtcgca cccgaggtgg 23640

agggcttcgc cagcacgggc tcggcgggca gcgtggcctc gggccgggtg tcgtacgtgt 23700

tcggcttcga gggcccggcc gtcacggtgg acacggcgtg ctcgtcgtcc ctcgtggcga 23760

tccacctggc cgcgcaggcg ctgcggcagg gcgagtgctc gatggccctc gccggtggcg 23820

cgacggtgat ggcgacgccg ggcaccttcg tggagttctc ccggcagcgg gccctcgcgc 23880

ccgacggccg gtgcaaggcg ttctcgtcgt cggcggacgg caccggctgg gccgagggcg 23940

cgggcgtagt ggtccttgag cgcctgtcgg tggcgcggga gcgcggccac cacgtcctgg 24000

ccgtgctgcg cggcagcgcg gtgaaccagg acggcgcctc caacggcctc accgccccca 24060

acggtccctc acagcagcgc gtcatccgca gggcgctcgc aggcgccggg ctcgcgccgg 24120

ccgacgtgga cgcggtggag gcccacggga ccggcacggc cctcggcgac ccgatcgagg 24180

cccaggcact cctggcgacg tacggacagg accgggaccc gcaacagccc ctgtggctcg 24240

gctcgttgaa gtcgaacctc gggcacacgc aggccgccgc cggtgtctcc ggtgtgatca 24300

agatggtgca ggcgctgcgg cacggcgtcc tcccggcgac cctccacgtg gccgcgccca 24360

ccccccaggt cgactggtcg gcgggcgcgg tggaactcct caccgaggcg cgggagtggc 24420

cgcgcaacgg gcgtccccgg cgggcggggg tgtcgtcgtt cggtgtgagc gggacgaacg 24480

cgcacgtgat cctggaggag gcccccgagg agccggtcca ggcaggggct gcttccgcga 24540

gcgcggtgcc gttggtggtg tcggcgcgga gcccgaggtc gctggcgggg caggcccgtc 24600

gcctcgtgtc gtacgtggag tccggcgccg acggggtgtc gctggcgggc gcggcccggg 24660

ccttggtgtc gggtcgggcc gtcctcggcg agcgtgccgt ggtggtggcc gactcgggcg 24720

aggaggcgct ggccgggctg cgggcgctcg cgcggggcga gagcggaccg aacctggtga 24780

ccggcagcgg aggtgtgccg ggcaaggtgg tgtgggtgtt ccccgggcag ggctcgcagt 24840

gggtgggcat ggggcgcgaa ctcctcgatt cctcttcggt gttcgtcgag cgggtcgcgg 24900

agtgtgctgc cgctttggag cggtgggtgg actggtcgct tgtcgacgtg ctgcggggtg 24960

aggtcgagcc cgcgttgctt gagcgggtgg atgtggtgca gcccgcgggc tttgcggtga 25020

tggtggggtt ggcttcggtg tgggcgtccg tgggtgtgaa gccggatgcg gtgcttggtc 25080

actcgcaggg tgagattgcc gcggcttgtg tggcgggtgc gttgtcgctt gaggatgcgg 25140

cgcgggtggt ggcgttgcgc agtcaggcca tcgcggcgcg gctggccggg cgtgggggca 25200

tggcctcggt cgccctcagc gaggaggacg cggccgggcg tctggagccc tgggccgacc 25260

gcgtcgaggt cgcggccgtc aacgggccgt cctccgtcgt ggtcgccggt gacgccgagg 25320

ccttggacga ggccctgcgg gagctctccg gtgacggcgt acgagtgcgg cgcgtggccg 25380

tggactacgc ctcgcacacc cgtcacgtcg aggacatcga ggacaccctc gccgaagccc 25440

tggccggggt cacggccaag gccccggtgg tgccgttcta ctcgaccgtc acgggcagtt 25500

gggtggagga cgccggggtc ctggacggcc ggtactggta ccgcaacctg cgcggacagg 25560

tgcggttcgg cccggccgtc cgcgaactgc tcggccaggg ccacggggtg ttcctggagg 25620

tgagtgctca cccggtgctc gtgcagccga tcaccgaggc cgtggacgcc gccgacgccg 25680

atgtggtggt gaccgggtcg ctgcgccgtg acgagggtgg cctgcggcgg ctcctggctt 25740

cgatggccga gctgttcgtg cggggcctgg ccgtcgactg ggccgccgtg ctgcccgcag 25800

gggccgcgcg tgcggagctg ccgacgtacg ccttcgacca cgagcactac tggctcgagc 25860

cggccgaccg ggccaccgac gcggcctcgc tggggcagac cgccggtgac catccgctgc 25920

tgggcgcggt cgtcggcatg ccggactcca gcggtctcat ggccacgtcg aggtggtcgc 25980

tccgctcgca gccctggctc gcggaccacc tggcgtcgga cgcggtcgtg gtgccgaacg 26040

cggccctggt cgaagtggcc gtccggctcg gcgacctggc cgccaccccc gtactcgacg 26100

aactcgccgt cgacgcgccc gtggtgctgc cgcggcgggg cagccgcggc atccaggccg 26160

tcgtcggtga gccggacggg gcgggacggc ggtcggtgga gatctactcc cgcgccgggg 26220

acgcccccct ggacgcgcgc tggacccggc acgcgcacgg gacgctgtcg acccgtgccg 26280

tcgaggtccc ggcggcggga cccgcgaccg aggtcgcgct tgacggtgcc gcgctccgcg 26340

acgcgggccg gtacggcgtg caccccgcgc tgctggaagc cgccgtccgt acggtcatcg 26400

ccgacggcac ggtcccggcg gtgtggaccg gagtgaccct gctggcgtcg ggcgccaccg 26460

cgctccacgt acgagcgggc gacaccacgc cggagggcac cggtctgcgg ctgaccgacc 26520

ccacggggca gcccgtcatg accgtcgcgt ccgtacgcgg ggcggcgttc tgccccgaac 26580

aggcagaaca ggcaggggcc gacggtgcgg tgacgcacga cgcgctgttc cgcgtcgact 26640

ggagggaact ggcgctgccc gccgtggagc gcccgccggc cagcgtgacc gtcgcgaccg 26700

gcgaggacgt caccaccacc gccgcgggtc cggtcgcacc tgatgtgctg ctgtacgaag 26760

ccgtgcgcgc ggccgccgac ccccgcgtcc cggtcaacgc ggccctcgcc gtgctccagg 26820

cctggctcgc cgagcccgcc ctggcgcgga cccggctcgc cgtggtcacc ggggaccacg 26880

ccgaactcga cgcggccgcc gtgtggggcc tggtgcgctc ggcccagtcg gagcatccgg 26940

gccggatcgt cctcgtggac ctcgacgagg cctcccggga cgccctgccc gccgcgctgc 27000

tgagcggtga accgcagctg agggtgcgcg acggcgtcgc gtcggtgccc cgcctcgcac 27060

gcgcggccgt cccggcgggc gcggcgcccg ccggagccgg gcggcggccc cggccgctcg 27120

accccgaagg caccgtactg atcaccggcg gcaccggcac gctcggcgcc gtgacggcac 27180

ggcacctcgt cgccgcgcac ggcgtccggc acctggtcct ggccggccgc cggggccagg 27240

ccccggagct gcgcgacgag ctgaccggga tgggggcctc cgtcaccctc gccgcctgcg 27300

acacggcgga ccgggcccag gtggcggcgc tgctgcgggc gatacccgcc gcgcaccccc 27360

tcaccgccgt ggtgcacgcc gccggggtcc tggacgacgg ggtgctcacc gaactgaccc 27420

cggaacgcct cgacaccgtg ctgcggccga agctggacgc cgcccggcac ctgcacgagc 27480

tgacccggga cctggacctc gccgcgttcg tcctcttctc ctcggcggcg ggcgtcctcg 27540

gcaacccggg ccaggccaac tacgcggcgg ccaacgccgc cctggacgcc ctggcgcgtc 27600

agcggcaccg ggcgggcctg cctgcggtgt ccctggcctg gggctactgg gcgacggcca 27660

gcggcatgac cgcgcacctg ggcgccgccg acctgcggcg caaccggcgc atcggcatgg 27720

tcggactgtc cccggccgag ggcatggccc tcctcgacgc cgccctgaac accccggcgg 27780

acgcggccgg aacgctcgtg gcggcgaagt tcgacctcaa cgcgctgcgg gccgcggcgg 27840

ccgaagagcc gctgccgccg ctgctgcgcg gcctcgcccc ggcaccgcgg cccaccgccc 27900

ggacggccgc accggccggg cccgcggcgc tgcgggaacg cgtcacaggc ctggccgccg 27960

ccgagcggac cgaggccctc gtcgacctgg tgcgccggca cgccgccgaa gtgctcgggc 28020

acaccaccgc cgacgccgtg cgggcggacc ggaccttcaa ggaggccggg ttcgactcgc 28080

tgaccgccgt ggagctgcgc aaccggctcg ccgccgcgac cgggctcacc ctctccccgg 28140

cgatggtctt cgaccacccg aagccgaccg cgctcgccgc gcacctgggc gatcggctcc 28200

tcggcgtggc ggcgcagcgc ccggccgggc ccggcgcgcc cgcaccgacc accgacgagc 28260

cgatcgccat cgtcgccatg gcgtgccgct tccccgcggg cgtgcacagc ccggaggacc 28320

tctggcgggt ggtggccgac gggatcgacg ccgtcaccga gttccccgcc gaccggggct 28380

gggacaccga ccggctcttc cacgcggacc ccgaccacga gggcaccacc tacgtgcgcc 28440

acggcgcctt cctcgacgac gccgccgggt tcgacgccgc cttcttcggc atctcgccga 28500

acgaggccct cgccatggac ccgcagcagc ggctgctcct cgaaacgtcc tgggaggcct 28560

tcgagcgggc cgcgatcgac ccgaccgcgc tggccgggca ggacgtcggc gtcttcgtcg 28620

gcgtcaacag ccacgactac agcgtgcgca tgcaccgggc ggccggtgtc gagggcttcc 28680

gcctcaccgg cggctcgggc agcgtcgcct ccggccgcgt cgcctaccac ttcggcttcg 28740

agggccccgc cctcacgatc gacacggcct gctcctcctc cctggtggcc ctgcacacgg 28800

ccgcgcaggc gctgaaccgc ggcgagtgct ccatggccct ggccggcggc gtgatggtga 28860

tgggcaccgt ggagacgttc gtcgagttct cccggcagcg cggactcgcc cccgacggcc 28920

ggtgcaaggc gttcgcggac ggcgccgacg gcaccggctg gtccgagggc gtggggctgc 28980

tcctcgtgga gcgcctgtcg gacgcccgcc gcaggggcca tcccgtactg gccgtcgtcc 29040

gcggctccgc cgtgaaccag gacggcgcgt cgaacggcct gacggccccc aacggccccg 29100

cgcagcagcg cgtcatccgc aaggccctgg cccacgcggg cctcggcacg caggacgtgg 29160

acgccgtgga ggcgcacggc acgggcacga cgctcggcga cccgatcgag gcccaggcgc 29220

tcctggcgac gtacggccag gaccggcccg cgggacggcc gctgtggctg gggtccgtga 29280

agtcgaacct cggccacacc caggcggcgg cgggcgtcgc cggtgtgatc aagatggtca 29340

tggccatgcg ccacgacacg ctccccaaaa cgctgcacgt ggaccgcccg tcgtcccgca 29400

tcgactggtc cgcgggcgcc gtggagctgc tgacacaggc ccgtgactgg ccgcgcgagg 29460

gccgtccgcg ccgggccgcc gtgtcctcct tcggcatcgg cggcaccaac gcgcacgtcg 29520

tcatcgaaga ggcccccggc acggcggcgg ccgagcccga ccccaggcgc gcggaggaac 29580

cgacggacct cccggtgccg gtgcccgtgt cggcgcggac gcccgccggg ctgcgcggcc 29640

aggccgaccg gctcgcccgc ttcctcgacg acaggaccga cttacgcctc accgacgcgg 29700

cgcacgccct ggccaccacg cgcgcccggc tcgaccaccg cgccgtcgtc ctggcctccg 29760

accggccgca gctcggagcg gacctccgcg ccgtcggccg gggcgaggag cgccccgcgg 29820

tcgtctccgg caccccggtc gcgggcaagc tcgccgtcct cttcaccggc cagggcagcc 29880

agtgggcggg catgggccgt caactggccg ccacgtaccc ggtcttccgc gacgccttcg 29940

ccgccgcgtg cgccgccgtg gaggcgcatc tgagcggcct cgcggcgcgc ccgctgcacg 30000

acgtggtgtt cgccgctccc ggggcgcccg gcagcgaact cctcgaccgg accatgtaca 30060

cgcaggccgc cctgttcgcc ctggagaccg cgctgttccg gctcttcgag tcctggggtg 30120

tccggccgga cctcctcgcc gggcactcga tcggagagct caccgccgcg cacgtctccg 30180

gagtgctcgg cctggccgac gcgggcgaac tggtagcggc ccgcggccgg ttgatgcagg 30240

ccctgcccga gggcggcgcg atggtcgccg tccaggccac cgaggccgac gtcgaaccgc 30300

tgctcgcccg ggcccatggc gcggtctgcg tggccgcggt caacggcccc gacgcgctgg 30360

tgctctccgg cgccgagaac gccgtgctcg ccgccgccga cgcactggcc cgccggggcc 30420

gcaagacgcg gaggctggcg gtcagccacg ccttccactc gccgctgatg gaccccatgc 30480

tcgacgagtt ccgcgcggtc gcggagcgcc tgtcgtaccg gccgggcgac cttcccgtcg 30540

tctccaccct gaccggtgac cttgcccagg acgggcagct cgccgacccc ggccactggg 30600

ccgaccaggt gcggaacacc gtccggttcg gcgacgccgt caccgcgctc gcccggcggg 30660

gcgcgacgac gttcctcgaa ctgggcccgg gcgggaccct cgccgcgatg gcgctcggct 30720

cgcccgacgc accggagcgg agctgcgtcg ccaccctgcg caaggacggc gcggaggccg 30780

tcgacgtcct gaccgcgctc gccgaactgc acgtgcgcgg cacgtccgtg gactggacgg 30840

ccgtactcgg acacccggcc gcggcggccg gaaccgagct gcccacctac gcgttccagc 30900

accagcggta ctgggtcgag tccgacgacg ccgcatcggc cgacgcggaa gccctcggca 30960

ccaccggcgc ccggcacccg ctgctcggca ccgtcatcga cgtaccgggc gacgacggcg 31020

gcaacggcag cggcggtgtc gtgctcaccg ggcgcctcgc accgcacggc ccgggccgcc 31080

tccccggccc ggccgcggcc gacggcacca cccggatccc ggccgccgta ctcctggaca 31140

tgatcgtcgg ggccggtgac gaagtgggct gcggcggcct cgaacaggtc gtcgtcgaga 31200

gcccgttgac ggttccggag cgcggccaca cccacgtgcg ggtgtccgtg accgcccggg 31260

gcccggacgg gcgccgccgc gccgccgtcc acggccgccc cgcagggccg gaacgaacgc 31320

cctggacgtg ccacgcccga gcggtcctcg tccccggcgc gccgcgcccg gccttcgacc 31380

tgcgggcctg gcagccgccc gcggacgcct ccgcgccgct ccccggaacg cgcgtgtggc 31440

agcaggacgg ccgcacctgc gcggaaatca cgctcccgga cgaactcgta gccgaggcgg 31500

ccgacttcgc gctgcacccc gtgctcgtcg acaccgcgct gcgcgtcctg gaccggagcc 31560

ccggccggga cctgtccgat tgcgccgcgc tcgcggtgta cgcggagggc gccacctcgc 31620

tgcggctgcg gatcacgccg cggccggacg gccgccacct gctggagctg gcggacgcgg 31680

acggtgaacc cgtcgcggcc ctcggcccgt tgagcctcga agccctgccc ggggagggtc 31740

ccgcgtcgcg tgattccgcg ccgtacgagg acgccggcgt accggcggtg cccggtgtca 31800

cccgccgcgt ggtcgcgcgc ggggaagaca ccggaggcgc catggccgac cggctgaccg 31860

accggctgac cggccgacgg accgaccagc tggccgaccg gctggccgac cggctggccg 31920

ggctcccggc cgcggagcag caccgcaggg tcctctcctt ggtccaggag agcaccgcgg 31980

tcgtgctcgg ccaccgccag ggcgaccgct tcgacgacga gcagcccttc aagaacctcg 32040

ggttcgactc cctcagcgcc gtcaagctcc gcaaccggct gcgcgacttc accggcgtcg 32100

acctgcccag ctccctggtc ttcgaccacc cgacccccgc cctcctcgcg gccttcctgc 32160

gcgccgaact gctcggcgag cagcccgcgg cacccgcccc cgccacggcg gcagcggctt 32220

ccgacgagcc catcgcgatc gtcgcgatga gcagtcggct gcccggcgga gcggacagcc 32280

ccgaagagct gtggcagctg ctcgtggagg gccgggacgc ggtctccggc ttcccggtgg 32340

accgcgactg ggacctggag gggctgtacc acccggaccc gggccacccc ggcacgagct 32400

acacgcgctc gggcggattc ctccacgagg ccgcgcggtt cgacgccggg ctcttcggca 32460

tctcgccgcg cgaggccctc gccatggacc cgcagcagcg gctgctcctc gaaacctcct 32520

gggaggccct ggaacgggcg ggcgtcgacc cgctgtcggc caggggcagc gacatcggcg 32580

tcttcaccgg gatcgtccac cacgactacg tgtcccggct ccaccgggtg cccgaggacg 32640

tgcagggcta cctcatgacc ggtacggcgt cgagcgtggc gtcgggccgg gtgtcgtacg 32700

tgttcggctt cgagggcccg gcggtcaccg tcgacacggc gtgctcgtcg tccctggtcg 32760

cgatgcacct ggccgcgcag gcgctgcggc agggcgagtg ctcgatggcc ctggccggtg 32820

gcgcgaccgt gatggccggt cccgacgcct tcctggagtt ctcacggcag cgcggtctgt 32880

ccgccgacgg ccggtgcaag gccttctccg cgacggccga cggcaccggc tgggccgagg 32940

gcgtgggcgt ggtcgtcctg gaacgcctct cggtcgcgcg ggaacgcggc caccaggtcc 33000

tcgcggtcat ccggggcagc gcggtgaacc aggacggcgc ctccaacggc ctcaccgccc 33060

ccaacggccc ctcgcagcag cgggtgatcc gccgggccct cgacacggcg gggctcgcgc 33120

ccgcggacgt ggacgtggtg gaggcccacg ggaccggcac ggccctcggc gacccgatcg 33180

aagcccaggc actcctggcg acgtacgggc agggtcgggg cgccgaacag cccctgtggc 33240

tgggctcgtt gaagtccaac atcggtcaca cgcaggccgc cgcgggcgtg gcgggcgtga 33300

tcaagatggt gctggcgctg cgccacgcgg tcatgccccg caccctgcac gtcgacgagc 33360

cgacggccga ggtggactgg tcggcgggcg cggtggagct gctgaccgag gccaggaact 33420

gggagcggaa cggccgtccg cgccgggcgg gcgtctccgc gttcggcgcc agcggcacga 33480

acgcccatct gatcctggag gaggcccccg aggaccccgt cgcggcggag accgtgccgg 33540

tgggtgtggt gccgctggtg gtgtcggcgc atagccaggc ttcgctggcg gggcaggccg 33600

gtcggctggt gtcgtacgtg gagtcgggga ctggtggtcc gtcgctcgcg ggagtggccg 33660

gggccctggt gtcgggccgg gccgtcctcg gtgagcgcgc ggtggtcgtg gccgattcgc 33720

ccaccgaggc gctgaccggg ctgcgcgcgc tggcccgggg ggagagcggt ccgcacctgg 33780

tgtccggcag cgcggccccg ggcgcgcccg gcaaggtcgt gctggtgttc ccgggccagg 33840

gctcgcagtg ggcgggcatg gggcgtgaac tccttgatgc ctccccggtg ttcgccgagc 33900

ggatcgctga gtgcgcggct gcccttgagc cgtggctgga ctggtccctc atcgacgtac 33960

tacgtggtga ggccgagccc gagttgttgg agcgggtgga cgtggtgcag cccgccagct 34020

tcgcggtgat ggccgggctg gcttccgtgt gggcgtccgt gggggtcgag ccggacgcgg 34080

tgctcgggca ctcccagggg gagatcgcgg ccgcctgcgt ggcgggtgcg ctgtcccttg 34140

aggacgcggc ccgcgtggtg gccttgcgca gccgaaccat tgccgcggag ctggcggggc 34200

gcggcggcat ggcgtccgtc gccctcggcg agcaggaggc ggccgcccgt ctggagcggt 34260

gggcggaccg ggtcgaggtg gccgcggtga acagcccgtc gtcggtggtg atcgcgggcg 34320

atgccgcggc cttggacgag gtcctggagg agctttccgg tgccggggtg cgggtgcggc 34380

gcgtggccgt ggactacgcc tcgcactccc ggcacgtcga ggacatcgag aacacccttg 34440

ccgaagccct ggcgggggtg aacgcgaagg ctccggtggt gccgttctac tcgaccgtca 34500

cggggagttg ggtggaggac accggggtcc tggacggctc gtactggtac cgcaacctgc 34560

gcgggcaggt gcggttcggc cccgccgtgg ccgacctgct ctcccagggc cacggcgtgt 34620

tcgtggaggc cagcgcccac ccggtcctgg tgcagcccgt caccgaggcc gtcgacggca 34680

cggacgccga agcagtggtg accgggtcgc tgcgccgcga ggacggcggc ctgcgacggc 34740

tcctcacgtc ggcggccgag ctcttcgtgc gcggagtcgc cgtcgactgg gccggtgtgc 34800

tgcccgccgg agccctggcc cggcccgcgc gcgcggacct gcccacgtac gccttcgacc 34860

gccggcacta ctggctgcgc gaggcggaga cgacggccga cgccgacggc gccgccgaca 34920

gctcggactc cgccttctgg tccgccgtcg agcaagccga cacggactcg ctcggcgccc 34980

tcctcgaaac ggcgtcgggc gaccagctcg gcgccctcgc caccctcgtg cccgtgctcg 35040

ccgagtggcg cgggaagcgc cgccgccgct ccgcggccga gaagctgcgc taccacgtca 35100

cctggcagcc cctcgcacgc gaagccaccg gagtgcccgg tggccggtgg ctggtcgtcg 35160

taccggccga acgcaccgac gacagcggca ccgacgcgct tctggacgaa ctgaccggcc 35220

tcgggctcga cgcgctcctc ctggaggtcg gcgcgtccga cggcggccgg gagcggctcg 35280

ccgagcggct ggccggagtc ctcgccgaag acgacgtgac cggtgtgctc tcgctgctcg 35340

ccctcgaccg gagcgcacgc ggcgccccgc aggaccccac cgccgtcacg gcggcggccc 35400

tcgccctcat ccaggcgctg ggcgacaccg gcgccgcccc gccgctgtgg tgcgtcacca 35460

ggggcgcggt gaacatcggc atccacgacg ccctcacctc acccgcgcag gcggccctgt 35520

ggggcctcgg ccgcgccgcc gccctcgaac ggctcgaccg ctggggcggc ctggtcgacc 35580

tgcccgcggc gacagggccg cgcacggccc gccagctcct cggcgtgctg aactccgccg 35640

cgggcgagga ccagctggcg gtccgcaggt cgggcgtgta cgcgcgcagg ctcgtccgca 35700

aggccgtgcc ggaggccgcg ggggacaccg gctggcagcc ccggggcacg gtcctggtga 35760

ccggcggcgc cgaaggactc ggcaggcacg tctccttgtg gctcgccctc gccggcgtcg 35820

accggctcct cgtcaccacc accccgcacg cccccgaagg gagtacggcc gaactccgct 35880

cccaaatcgc cgggttgggc ctcgccaccg tcgtggagtc ctgcgcggac accgaccggg 35940

acgcgctcgc gcgcctcgtc tccgcgacgc ccccggagca gccgctgacc gccgtcgtgc 36000

acgcggccga catcacgcgg accagtaccg tcgacgacac cggcgcacac gacctcgccg 36060

aggtgttcgc ggccaaggtg gacacggcgg tgtggctgga cgagctgttc gaggacacgc 36120

cgctcgacgc gttcgtcgtc ttctcctcca tcgccggagt gtggggcggc ggcggccagg 36180

ggccgtccgg cgcggcgaac gccgtcctcg acgccctggt cgaatggcgc cgggcccggg 36240

ggctgcgggc gacgtcgatc gcctggggag ccctcgacgg gatcggcgtc ggcatggacg 36300

aggccgccct cgcccagctc cgccgccggg gcgtcctgcc catggcgccg cagctggccg 36360

tcaccgcgat ggtgcaggcc gtgcagggcg acgagaagtt cgtgacggtc gccgacatgg 36420

actggggcgc cttcgtcccc gccttcactt cggtccgcgc cagccccctc ttcgccgacc 36480

tgcccgaggc gaaggcggtc ctcgacgcgg cgcgggacga cggcgaggac agcgacaccg 36540

cggcctccct cgcggactcc ttgcgggcgg tccccgacgc cgagcagcac cgcctcctgc 36600

tgcgcctggt ccgcggccac gcctcgacgg tcctcggcca cggcggcgcg gagggcatcg 36660

gcccgcgcca ggcgttccag gaggtcggct tcgactccct ggccgccgtc aacctgcgca 36720

acagcctgca cggggccacc ggactgcgcc tgcccgccac gctgatcttc gactacccca 36780

ccccggaggc gctggtcggc catctgcgca ccgaactcct caaggaagcc gacgacggcc 36840

tcgcggggcg ggaggacgac ctcaggcggg tcctggcgtc cgtgccgctc acccggttca 36900

aggaggccgg ggtgctcgac gccctcctcg gcctcgccga gaccgagacc gggaccgggg 36960

ccgaggcgga gaccgacacc ggggccgccg cgggcgcggc tacccgtacg ggcgccgcgg 37020

acgcggagct gatcgacgcg atggacgttg ccgacctggt gcaacgggcc ttgggcaaga 37080

cgagctgacc acctgtgaac gagtcccgga aggatggcta tgtcggcgcc agacgagcag 37140

gtcattgagg cactgcgtgc ctcgctgaag gagaacgccc ggcttcagca ggagaacagc 37200

acactcgtcg cggccgccgc ggagcccgtc gcgatcgtct ccatggcctg ccgctacgcc 37260

ggtggcgtcc ggggcccgga ggacctctgg cgcgtggtgt ccgaaggcgc cgacgtctac 37320

ggccccttcc ccgacgaccg gggctgggac gtcgagggcc tctaccaccc cgaccccgac 37380

caccccggta cgacgtacgt gcgcgagggt gccttcctgc acgacgcggc gcggttcgac 37440

gccgggttct tcggcatctc gccgcgcgag gccctcgcga tggacccgca gcagcggcag 37500

ctcctcgaag tgtcctggga gaccttcgaa cgcgccggga tcgaccccca cgcgatgcgc 37560

ggcggtgacg tcggcgtgtt cgccgggatc gtgcaccagg actacgcccc ggacctcagc 37620

ggattcgagg ggtacctaag cctggagcgc gccctgggca ccgcgggcgg cgtcgcctcc 37680

gggcgcgtcg cgtacaccct gggactcgaa ggccccgcgg tgaccgtcga caccatgtgc 37740

tcctcgtccc tcgtggcgat ccacctggcc gcgcaggcgc tgcgcagggg cgagtgcacg 37800

atggcgctcg cgggcggctc gacggtgatg gcgacgccgg gcggcttcgt cggcttcgcg 37860

cggcagcggg cgctcgcctt cgatggccgc tgcaagtcct acgccgccgg agccgacggc 37920

tccggctggg ccgagggcgt gggcgtgctg ctccttgagc gtctttcggt ggcccgggag 37980

cgcggccacc aggtcctcgc ggtgatccgc ggcagcgcgg tgaaccagga cggcgcctcc 38040

aacggcctca cggcccccaa cggtcccgcg cagcagcggg tgatccgcaa ggcgctggcc 38100

ggcgccgggc tcgccccgtc ggacgtggac gcggtggagg gccacggcac cggcacggtc 38160

ctcggcgacc cgatcgaggc ccaggcgctg atggccacgt acgggcaggg ccgggacccg 38220

gagcggccgc tgtggctcgg ctcggtgaag tccgtcatcg gccatacgca ggcggcgtcc 38280

ggcgtggccg gtgtgatcaa gatggtgcag gcgctgcggc acggcgtgct cccggcgact 38340

ctgcacgtgg acgcgcccac cccgcaggtg gactggtcgg ccggggcggt ggagctgctg 38400

accgaggcca ggggctggga gcgcaacggc cgtgcgcgcc gggcgggcgt ctccgcgttc 38460

ggcgccagcg gcacgaacgc ccatctgatc ctggaggagg cccccgaggc cccggtcacg 38520

gaggagagcg cgccttcggg cgtggtgccc ctggtggtct cggcgggcac tgcggcttcg 38580

ctgacggagc aggccgaacg cctcgcctcg tacctggagt cgggtaccga cggggcgtcg 38640

ctcgcggcgg tcgccgggac gctggccgcg ggccgggccg tcctcggcga gcgcgcggtg 38700

gtggtggcgg gcacgaccga cgaggcactg accggcctgcgggcactcgc ccgcggcgag 38760

aacgcgaccg gcctcgtcac cggcacgggc acgccgggca aggtggtgtg ggtgttcccc 38820

gggcagggtt cgcagtgggt gggcatgggg cgcgaactgc tcgattcctc tccggtgttc 38880

gtcgagcggg tcgcggagtg tgctgctgct ctggagcggt gggtggactg gtcgcttgtc 38940

gacgtgctgc ggggtgaggt cgagcccgcg ttgcttgagc gggtggatgt ggtgcagccc 39000

gcgagctttg cggtgatggt ggggttggcg gcggtgtggg cgtctgtggg tgtcgagcct 39060

gatgcggtgc tcggtcactc gcagggtgag atcgcggcgg cttgtgtggc gggtgcgttg 39120

tcgcttgagg atgcggcgcg ggtggtggcg ttgcggagtc aggcgatcgc ggcgcggttg 39180

gccgggcgtg ggggcatggc ctcggtcgcc ctcagcgagg aggacgcggc cgggcgtctg 39240

gggccctggt cggatcgggt cgaggtcgcg gctgtcaacg ggccgtcctc cgtcgtggtc 39300

gccggtgacg cccaagccct ggacgaggcc ctggaggccc tggacgacca gggcgtccgc 39360

atccggcgga tcgcggtgga ctacgcctcg cacacccggc acgtggagga cgtccgcacc 39420

acgctcgccg aggtgctcgc cgggatcggc gcacaggagc cgacggtgcc gttctactcc 39480

accgtcaccg gcggttgggt gacggacgcc ggggccctgg acagcgccta ctggtaccgt 39540

aacctgcgcg aacgcgtacg gttcggcccg gccgtcgccg acctgctcga ccaggggcac 39600

ggggtgttcg tggaggtcag cgcccacccc gtactggtcc agcccatcac cgaggccgtg 39660

gtcgccgccg acgccgacgt ggtggtgacg ggctcgctgc gccgcgagga cggcggcctg 39720

cggcgcctcc tcacctcgat ggccgagctg ttcgtccggg gcaccccgct ggactgggcc 39780

agggcgctgc ccgccggggc cacgtcccgg cgcgtcgacc tgccgacgta cgccttcgac 39840

caccagcact actggatccg gatgggcgcg tccgccacgg actccgcgtc cctcggcctc 39900

gccggggccg accacccgct gctgggcgcg gtcgtaccgc tgccgcagtc cgacggcctc 39960

gtcttcacct cacgcctctc gctgcggacg cacccgtggc tcgccgacca cgagatcggc 40020

ggcgcggtcc tcgtccccgg caccgtgtac gtcgatctcg cggtgcgcgc gggcgacgag 40080

ttcggccacg gcctcctgga ggaactcgtc atcgaggcgc cgctggtgct gcccgacagc 40140

ggcggcgtac gactccaggt cgcggtgagc ggacccggcg cgacgggttc gcggaccgtg 40200

gacgtgtact cccagcgcga ggacgccgcc gacgacggcg gcgcggacga cgtgtggacg 40260

cggcacgcca ccggcctcct ggcggcctcg ccgacggcgc ccgacccggg gcgcgcgttc 40320

gacttcgcgg cctggccgcc gcccggcgcg gagccggtcc cgatgacgga cttctacgcc 40380

gacctggtcg agcgcggcta cgcgtacggg cccgccttcc agggcctgcg cgcggtgtgg 40440

cgacgcggcg acgaggtctt cgccgaggcc gcgctgcccg aggaacaccg agaggaggca 40500

ggcgggttcg gcatccaccc ggcgctcctc gacgccgccc tccacacgaa cgccttcgcc 40560

cgcccggacg acgaccgcaa ggtgctgccg ttcgcctgga acgggctgct cctgcacgcc 40620

gtgggcgcct ccgcgctgcg cgtgcgggtc gcaccgtgcg gcccggacgc gctgtcgttc 40680

cacgccgccg acgacaccgg cgccacggtc ctgacgatgg actcgctggt gtccctgccg 40740

gtctccaccg accagctgga cgccgcggcg gcggacggga gccgcgacac gctgttcggc 40800

gtggagtgga ccgaactgcc gccggtacga ggggatgtgg ccggaagcgc gacggccccg 40860

ccgttggccg tcctggaggc cttcggggac gacggcgagg gcgcggtact cgccctgacc 40920

acccgcgtgt tgggggcggt gcaggagtgg cttgccgggg cgggctctga cgattcgcgc 40980

cttgtggtgg tgacccgggg ggcggtgccc gcgggcgacg gagcggtgac ggatccggct 41040

ggagcggcgg tgtggggcct ggtgcgtgcc gcgcaggcgg agaatccgga acggatcctg 41100

ctcgtcgaca ccgaccccgc ggccggagga gacctggatg cggcgctggg ggtggcgctg 41160

gccagtggtg agccgcaact cgcggtgcgt ggtgcgattt tctcggtgcc caggctggtc 41220

cgtgttgcgg gtcgggggct gggttcgggg aacgtattcg ccgctggaaa gaccgttcta 41280

cttagtggtg gaacaggatc cctgggtggc ctggtggccc gccacttggt cgcgcggcat 41340

ggcgtccggc atctgctgct tgcgagtcgg cggggtgcgg atgccgaggg catgccggag 41400

ttggtggccg agctcgccga gcagggcgcg gtcgtgtcgg tagtggcctg cgacgtgtcc 41460

gaccgcgacc aggtccgggc cctgctggcc gccgtaccgg acgagcaccc cctgacgggc 41520

atcgtccaca tggccggagt gctcgacgac ggagtgattg ggacgctgac gccggagcgc 41580

ctcgcgggcg tgttcgcgcc gaaggtggac gcggtcagcc acctcgacga gctgacccgc 41640

gaactgggcc tggaactgga cgcgttcgtg gtgttctctt ccgctgcggc gctcatgggg 41700

tcggcaggcc agggcaacta cgcggcggcg aacgcctacc tcgacgggct catgtccggc 41760

cgccgggcgg cgggcctgcc cgggatctcg ctggcctggg gcctgtggga gcagaccgct 41820

ggcctgaccg cgcacctcag cagcgtcgac caggcgcgga tgagccgcgg cggcgtcctc 41880

gcgatgaccc cggccgaggg cctggccgtc ttcgacatcg gcctgcacgc gggccaggcg 41940

ctcctcgtgc cgatcaagct ggacctgcgcacgctgcgga cccaggcggc ggcgggcggc 42000

ggggtgccgc agctgctgcg cggcctcgtc cgcgcgggcc gccgcgtggt acgggcggcg 42060

gccggggaca gcggcggcct ggtccgccgc ctcgccgggc tgcccccggc cgagcaggag 42120

aacctcctgc tgtccctcgt ccagggcgag gcgggcggcg tgctcggttt cagcggcccc 42180

gaactgaccc agggaacaag gggattcagc gacatcgggt tcgactccct gaccgccgtc 42240

gaactgcgga accggctgag cgcggcgacc ggcgtgaaac tgcccgccac cctgatcttc 42300

gactacccga ccccggtggc gctcgcccgc catctgcggg aggagctggg cgacacggcg 42360

gacggcgccg ccgcaccggc cgcggtcgcc gtcgcggacc cggacgagcc gatcgcgatc 42420

gtcgggatgg cgtgccgact gcccggcggg gtgacggacc ccgacggcct gtggcggctg 42480

gtgcgcgagg gccgcgaggg catgtcggac ttccccgacg accggggctg ggacctggag 42540

ggcttgttcg acgcggaccc ggacaacgcc ggtacgtcgt acgccagtca gggcgggttc 42600

cttgagggtg cggggctctt cgacgccggg ttcttcggca tctccccgcg cgaggccctg 42660

gccatggacc cgcagcagcg gctgctcctc gaaacctcct gggaagccct ggaacgggcc 42720

ggaatcgacc cgaccacggc acgcggcgcc gacatcggcg tcttctccgg cgtctccatc 42780

cacgactacc tggagtccct cagcaacatg cccgacgagc tcgaaggctt cgtcacgacc 42840

gccacggcgg gcagcgtcgc gtcgggccgg gtgtcgtacg ccttcggctt cgagggcccg 42900

gcggtcacgg tggacacggc gtgttcgtcg tccctggtgg cgatgcacct ggccgcgcag 42960

gcgctgcggc aaggcgagtg ctcgatggcg ctcgccgggg gcgtcgccgt gatggggtcc 43020

ccgatcggcg tcctcggcat gtcccggcag cggggcctgg ccgccgacgg ccggtgcaag 43080

gcgtacgcgg acggcgcgga cggcacggtg ctctccgagg gcgtcggcct ggtggtcctt 43140

gaacgcctgt cggtggcgcg ggaacgcggg caccgggtcc tcgcggtgat ccggggcagc 43200

gcggtgaacc aggacggcgc ctccaacggc ctcaccgccc ccaacggccc ctcccagcag 43260

cgagtgatcc gcaaggctct ggcgaacgcg ggcctctccc cgtccgaggt ggacgtggtg 43320

gagggccacg gcaccggcac caccctgggc gaccccatcg agacgcaggc gctcctcgcc 43380

acctacggga aggggcggga tgccgaacag ccgctgaggc tggggtcgtt gaagtccaac 43440

atcggccata cgcaggccgc cgccggtgtg tccagcgtga tcaagatggt ccaggcgctg 43500

cggcacaacg tcatgccgcc cacgctgcac gcacaggagc ccacccacca ggtcgactgg 43560

tcggcgggcg ccatcgagct gctgaccgag ccccgcgact ggccgcgcaa cggccgcccg 43620

cgccgggccg gagtgtcctc gttcgggatc agcgggacga acgcccatct gatcctggag 43680

gaggcacctg aggagccgct ttcggaggag ccggcttcgg tgggtgtggt gccggtggtg 43740

gtgtcggcgc gtagtgcggc ttcgctggcg ggacaggccg gtcgcctcgc gtcgtacgtg 43800

gagtcgggcg ggggtgcctc gctggcaggc gtggccgggg cgttggtgac cagccgggct 43860

gccctggacg agcgtgcggt ggtggtggcg ggttcgaacg cggaggcact cggcgggctg 43920

cgggcgttgg ctcggggcga ggacgcggcc ggtgtggtgt ccggcaaggt cgcgggttcc 43980

ggtgtgccgg gcaaggtggt gtgggtgttc ccggggcagg gcgctcagtg ggcgggcatg 44040

ggtcgcgaac tcctcgacac ctcaacggtg ttcgccgagc ggatcgctga gtgcgcggcc 44100

gcccttgagt cgctggtgga ctggtcgctc gttgacgtac tgcggggtga ggtcgagccc 44160

gcgctgcttg agcgggtgga tgtggtgcag cccgcgagtt tcgcggtgat ggtgggtctg 44220

gcttcggtgt gggcgtccgt gggtgtgaag ccggacgcgg ttcttgggca ctcgcagggg 44280

gagatcgcgg ccgcctgtgt ggcgggggcg ttgtcccttg aggacgcggt gcgggtggtg 44340

gcgttgcgca gccaggccat cgctacggag ttggccgggc ggggcggcat ggcgtccgtt 44400

gccttgagcg cggacgaggc agccgcgcgc cttgaggagt gggcgggccg ggttgaggtc 44460

gcggcggtca atgggccgtc gtcggtggtg gtcgcgggcg atgccgaagc cttggacgag 44520

gcgctggagg ccctggcggg cgacggtgtc cgaacccgcc gcgtggccgt ggactacgcc 44580

tcgcacaccc ggcatgtgga ggacatccgc gacaggctcg ccgaggcgct ggcgggggtg 44640

agtgcggagg ctccggtggt gccgttctac tcgaccgtta cgggcagttg ggtggaggac 44700

gcgggggtgt tggacggcgg gtactggtac cgcaacctgc gcgggcaggt gggcttcggc 44760

cctgcggtgg ccgatctgct ggcgcagggg catggggtgt tcgtggaggt cagcgcccac 44820

cccgtactgg ttcagcccgt cagcgaggct gtggatgaga gcggtgccga tgccgtggtg 44880

acagggtcgc tgcgccgcga cgaagggggc ttgcggcggc tgctgatgtc gatggcggag 44940

ctgttcgtac ggggtgtggc ggtgggctgg tcggccgtgc tgcccggcgg ggccggggtc 45000

gagatcgcgc gcgtggagtt gccgacgtac gccttcgacc atcagcacta ctggctcaag 45060

acggctgccc aggtcaccga cgcggcgtcg ctggggcagg cggcggcgga tcatccgctg 45120

ctgggtgcgg tggtgcggct gccgcagtcc gacgggctcg tcttcacctc gcgcctgtcg 45180

ctgcgcacgc acgcctggct ggccgaccac cgggtcggcg acgtggtgct ggtcccaggc 45240

accagtctgg tggagctggc ggtgcgggcc ggtgacgagg ccgggtgcgg ggtgctggac 45300

gaactcgtga tcggggaacc gttggtggtg cccgagcgcg gcggggtgcg ggtgcaggtc 45360

gcggtgggcg ggccggggga gaacggtgcg cgcacggtgg aggtgtactc ccagcgcgag 45420

gacgcgggcg atgaagccga cgcgtggacg cggcatgcca ccggcgtcct gtccgccgcc 45480

accgaccggt ccacgggcag cggcacgggc ttcgacttca cggcctggcc gccgccgggg 45540

gccgagccgg tggggatcgg ggagttctac gccgacctcg ccgagcaggg gtacgcgtac 45600

ggtccggcgt tccagggcgt gcgggcggtg tggcggcgcg gcgaggaact gttcgcggag 45660

gtcgcgctgc cggaggagca gcacaaggaa gcgggcaggt tcggcatcca ccccgcactc 45720

ctcgacgcgg ccctgcacgc cggggagccg ggcgccgcgg ccgaaggcgt caaggagctg 45780

cggcaaccgc tcgactggaa cggcctggtg ctgcacgccg cgggcgcctc ggcgcttcgg 45840

gcgcggcttg tgcccgccgg gcccgacgcc gtgtcgttgg aggcggcgga cgaagccggt 45900

ggcctggtgg tgacggcgga atcgctgacg tggcgagagg tgtccaccga ccggccgggc 45960

gcggcggaca cgctggtcgc ccagtcgctg ttcggcgtgg tgtggaccga gctgcccccc 46020

acgcagggcg cgattacgtc gctgtcgtgg atgccggtgg ccgccgccga cgaggtggcg 46080

acgctgaccg atgaagtggc ggcgggcgcg gccgccccga tggcggcggt cctggaggcg 46140

ttcggcggcg gcgacggtga ggaactggcc ttgtcctccc acgtgttggg agtcgtgcag 46200

gggtggttga acggggccgg gcttgaggag tcacgccttg tggtggtgac gcggggtgcg 46260

gtgcccgctg gtgaaggtgc ggtgacggat ccggcggcgg ccgccgtgtg gggtctggtg 46320

cgtgcggcgc aggcggagaa cccggacagg atcgtcctgc tggacctcga cccggcctcc 46380

gacgacgcag cgggctccgt actggacacg gtgctgggag ccgtcctggc caccggcgag 46440

cctcaaatcg cggtacgcgg aacggccttc tcggcgcccc gactcgtccg agtgccagcg 46500

caagccccgg acagccagtc ggtgttcaca tccgagggga ccgtcctcat caccggtggc 46560

accgggtcgc tcggtgctct cgtggcccgt cacctcgtca cccggtacgg cgtacggcgg 46620

ctgctcctcg ccagcaggcg gggccgggac gccgagggcg cgccggagct ggcggccgaa 46680

ctcgccgagc tgggcgcgac cgtctcggtc gtggcgtgcg acgtgtccga ccgcgaccag 46740

gccaaggccc tgctcgcgtc ggtatcggac gaccacgaac accggctgac cggcatcgtg 46800

cacacagcgg gcgtgctgga cgccggggtc atcggagcgc tgacgccgga gcgcctggag 46860

cgggtgttcg cgccgaaggt ggacgcggta cggcacctcg acgagctgac ccgcgaactg 46920

ggcctggacc tggacgcgtt cgtcgtctac tcgtccgtgt ccggcgtctt catgggcgcg 46980

ggcagcggca gctacgccgc cgccaacgcc tacctggacg gcctgatggc caaccggcgg 47040

gcagcgggcc tgcacggcct gtcgctggcc tggggcctgt gggcgcagac cagcggcatg 47100

gcggccaaca ccgacgacct caccaggaac cggatgaacc gccggggcgg cctgcaagcg 47160

atgtcgctga cggaaggcat ggagctgttc gacgccgccg tggggacccg ccagtccttg 47220

ctggtaccgg ccaggctcga cctgcgcggc gtacgcgccg acgcggcggc cggtggcggc 47280

gtgccgcaca tgctgcgcgg cctcgtacgc gccggccggc aggcggcacg ggcggcggac 47340

ggcggcgaca agcggcgccg gctgaccgac cggctcaccg ggctcgcacc ggcggaacaa 47400

gaagccctgc tcctcgacct ggtccgggcc cgggccgcgg tcgtgctcgg gcacgccggg 47460

ccctcgggcg tgcagccgga catggcgttc aacgaggtcg gattcgactc gctcacggcg 47520

gtggagctgc gcaacaggct gcgcgaggcg accggcctga agctgcccgc caccttgatc 47580

ttcgactatc cgaacccgct ggccctcgcc cgctacctgc accgcgaact gctcccggac 47640

gacggcgcga acggacagga gctggacgag gcgcggctgc ggcgcgccct cgcctccctc 47700

ccgctggccc gcttccgggc ggccggactg atggacgccc tggtcgagct ggccgccctc 47760

gccgacggcg caccgcacac ccgcacgacc gacgagaaca gcgaggccga ggggggcgac 47820

gagaagcggg cgatcgccga gctggacgtc gacgacctcg tgcacctggc gctcggcgac 47880

tgaggacctt tcgaaaccgc tggctgattg gggaattcaa gtgagcgcgt catatgaaac 47940

agtcgtccag gcgctacgga agtcgctgga agaagtcggc tcgctgaagc agcagaaccg 48000

ccgtctccgc gacgcttccc gcgagcccgt ggcgatcgtc ggcatggcgt gccggttgcc 48060

cggcggcgtg gcgggcccgg aggatttgtg gcgcctggtg gccgagggtc gggacgcggt 48120

gtcgggcttc cccgaggacc gcggctggga cctggagggc ctcttcgccc cggaccccga 48180

gagcgcgggc acgtcctata cccggcaagg cggattcctg cacgaggcgg ggctcttcga 48240

cgcggggttc ttcggcatct cgccgcgcga ggcgatggcg atggatccgc agcagcggct 48300

gctcctcgaa acgtcctggg aggccctgga ggcggccggg atggacccgg gctcgctgaa 48360

gggcgccgac gtcggggtgt tctccggggt gttcacgcag gggtacgtgg ccccgggagc 48420

cagcggggtg acgccggaag tggagggctt cgcgggcacc ggcggctcga cgagcgtggc 48480

ctcgggccgg gtgtcgtacg tgctcggttt cgagggcccg gcggtcacgg tggacacggc 48540

gtgttcgtcg tccctggtgg cgatgcacat ggcggcccag gcgctgcggc agggcgagtg 48600

ctcgatggcc ctggccggtg gtgcgacggt gatggcgacg ccgggcgcct tcgtggagtt 48660

ctcgcggcag cggggcctgg ccgccgacgg ccggtgcaag gcgttctcgt cgtcggcgga 48720

cggcaccggc tgggccgagg gcgtgggcgt agtggtcctt gagcgcctgt cggtggcgcg 48780

ggagcgaggc caccggatcc tcgcggtgct gcgcggcagc gcggtgaacc aggacggcgc 48840

ctccaacggc ctgaccgccc ccaacggccc ctcacagcag cgcgtgatcc gaggcgccct 48900

ggcgaacgcg ggcctgtccc cgtccgaggt ggacgtggtg gagggccacg ggaccggcac 48960

caccctgggc gaccccatcg aggctcaggc gctcctggcg acgtacgggc gcggccgcga 49020

ccccgaacag ccgctgtggc tcggctcgtt gaagtccaac atcggccatg cccaggcggc 49080

cgccggtgtg gccggtgtga tcaagatggt gcaggcgctg cggcacggcg tcctcccggc 49140

gaccctccac gtggccgcgc ccacccccca ggtcgactgg tcggcgggcg cggtggaact 49200

gctgaccgag acccgggagt ggccgcgcga cgggcgcccg cgccgggccg gtgtgtccgc 49260

cttcggcatg agcgggacga acgcccacct gatcctggag gaggcacccg agcagccgct 49320

ccccgaacac gcggtgcccg cgggggtggt gccgctggtc gtgtccgcac ggagccagac 49380

atcactcacg ggccaggcgg aacggctgat cacgcacctg gagtccgccc ccgacggcac 49440

ggcaccgctg gccccggttg ccggggcgct ggtgtcgggt cgtggggtgt tcggcgagcg 49500

tgcggtggtg gtggcgggtt cggagggcga ggcggtgtcc gggttgcggg cgctggcgcg 49560

gggcgagagt gatgtgaacc tggtgtctgg gagcgggggt gtgccgggga aggtggtgtg 49620

ggtgttcccg gggcagggct cgcagtgggc gggcatgggg cgtgaacttc tcgactcctc 49680

gccggtgttc gccgagcggg tgggggagtg tgctgcggcg ctggggccgt gggtggactg 49740

gtcgctcgtc gacgtcctgc ggggtgacgt cgagcccgcg ctgcttgagc gggtggatgt 49800

ggtgcagccc gcgagctttg cggtgatggt ggggttggct tcggtgtggg cgtccgtggg 49860

tgtgaagccg gatgcggtgg ttggtcactc gcagggtgag attgcggcgg cttgtgtggc 49920

gggtgcgttg tcgcttgagg atgcggtgcg ggtggtggcg ttgcggagtc aggcgatcgc 49980

ggcgcggctg gcggggcggg gtggcatggc gtccgttgcc ctcgcagagc aggaagtggc 50040

cgggcggctg gagccgtggg ccgagcgggt cgaggtcgcg gccgtcaacg ggccttcctc 50100

cgtggtgatc gctggtgatg ccgaagcctt ggacgaggtc ctgcaagtgc tctccgacga 50160

cggcgtacga gtgaggcgcg tggctgtgga ctacgcctcc cacacccggc acgtcgaaga 50220

catccaggac accctcgccg aagccctggc cggggtgagc gcgcaggctc cggccgtgcc 50280

gttctactcg accgtcacgg gcagttgggt ggaggacgcg ggggtgttgg acggcgggta 50340

ctggtaccgc aatctgcgcg gtcaggtggg cttcggcccc gcggtggccg atctgctggc 50400

gcaggggcac ggggtgttcg tggaggtcag cgcgcacccc gtactggtcc agcccgtcag 50460

cgagatcgtg gacgagagcg gtgccgacgc cgtggtgacg ggctcgctgc gccgtgacga 50520

agggggcttg cggcggctgc tggtgtcgat ggcggagctg ttcgtacggg gcgtggcggt 50580

cgactgggcg gccgtgctgc ccggcggggc cgggggtgat accgcgcgcg tggagttgcc 50640

gacgtacgcc ttcgaccacc ggcactactg gctcaagcag ggagcgtcgg ccaccgacgc 50700

ggcgtcgctg gggcaggcgg cggcggatca tccgctgctg ggcgcggtgg tgcggctgcc 50760

gcagtccgac gggctcgtct tcacctcgcg cctgtcgctg cgcacgcacg cctggctggc 50820

cgaccaccgg gtcggcgacg tggtgctcgt accgggcacc ggtctggtgg agctggcggt 50880

gcgcgccggt gacgaggccg ggtgcggggt tctcgaagag ctggtcgtcg aggcgccgct 50940

ggtcgtgccc gagcgcggcg gggtgcgggt gcaggtgtcg ctgggcgggc cgggtgagaa 51000

cggtgtgcgc acggtggagg tgtactccca gcgcgaggac gcgggcgatg aagccgacgc 51060

gtggacgcgg cacgccaccg gcctcctctc ggcagcggca cggccggacg gcagcagtgg 51120

cggcagtagc ggcggtggcg gcagcagtgt ggacttcacc gcctggccgc cgcgcggcgc 51180

gcagccggtc gaggtggggg acttctacga agcgctggcc gagcgcggat acggctacgg 51240

cccggcgttc cagggcgtgc gggcggtgtg gcggcgcggg gacgaggtgt tcgcggaggt 51300

cgtgctgccg gaggagcagc gtaaggaggc gggtaggtac ggcatccacc cggccctgct 51360

cgacgcggcc cttcaggcgg gcacgatcgg cgccgctgcg ggtccggcgg tggagggttc 51420

cggggagccg gtgctggcgt tcgcgtggaa cggcttggtg ctgcacgccg ggggtgcttc 51480

ggcgctgcgc gtacgcgtcg cgcccagtgg tccggacgcg ctgtcggtcg aggcggcgga 51540

cgaggccggt ggcctggtgg tcacgatgga ctcgctggtg tcccggccgg tgtcggcgga 51600

ccagttgggg gcggcgacgg ccgctcactc gctgttccaa gtggagtggg ccgaggtgtc 51660

gcaagagccg gagccgtccc cgttgtgggc accggtcacc acccccgacg acgtggcgct 51720

cctgcccggc aacgggccgg cggtggccgt catggaagcc ctcggaggcg acgaggaaga 51780

cgcggtactg accctgacgt cccaggtgct ggcggtcctc caggcgtggc tggccgaggc 51840

cgccctcgag gagtcgcggc tggtggtggt gacgcggggt gcggtcgccg ccggtggtga 51900

ggacatggtc acggatccgg cggcggccgc cgtgtggggt ctggtgcggg ccgcccagtc 51960

cgagaacccg gaccggatcg tcctcctcga caccgacccc acggcggaca cggctccgga 52020

cacaactccg gacacgtctc cggacgcggt actgggtgcg gtcctggcct gcggagagcc 52080

ccaggtcgcg gtgcgcggag gcgcgctctt gctgccccgg ctcggccgcg tcgccggtca 52140

tgtcacggac ggccccgcgg tgttcgggcc ggagggcacc gtcctgatct cgggcgccgg 52200

atcgctcggc gccttggtgg cccggcatct cgtcacccgg cacggagtgc ggagcctgct 52260

gctcgccagc cggagcggca ccgacgccga gggtgtgccg gaggtcatcg cggagctcac 52320

cgaacagggc gcgaccgtat cggccgtggc ctgcgacgtg tccgatcgtg atcaggtgaa 52380

ggcgctcctt gcggccgtac cgcaggagca ccggctcacg ggtgtcgtcc acacggcggg 52440

cgtcttcgac gacgggctga tcgcggcgct gtccccggag cgcctggagc gggtgttcgc 52500

gccgaaggtg gacgcggtgc ggcacctcga cgagctgacg cgcgacctgg acctcgacgc 52560

gttcatcgtg ttctcgtccg tcgcgggcct gtccggcggc gcgggccagg gcagctacgc 52620

cgcggccaac gccttcctcg acggcctgat ggccagccgc cgggcggccg gtctgcccgg 52680

cctgtcgctg gcgtggggcc tctgggagga gagcctcggc atggccgccc gcctcagcag 52740

cgtcgaccgg gcgcgggcga gccgcggcgg cgtgcgggcg atcgcggcgg ccgagggcat 52800

ggcgctcttc gacgccgccg cggggtccgg gcagtcactg ctggtgccca tcaagctgga 52860

cctgcgcgga ctgcgcgccg acacggcgtc cggcggggga gttccccctc tgctgcgcgg 52920

cctcgtacga gcgggccggc aaccggcgcg ggcggcggac acaggcgacg ggcaccggcg 52980

cctttccgcc cgcgtcgccg ggctcgcggc ggccgaacag gaggccttcc tcctggacct 53040

ggtccgcgcc gaggccgcgg tcgtgctcgg gcacaccggg ccgggaggcg tgcgcgagga 53100

ggtcgcgttc agggacgccg ggttcgactc cctcacggcg gtggagctgc gcaacaggct 53160

gcgcgaggcg accggcctga agctgcccgc caccctggtc ttcgactacc cgaccccgct 53220

ggccctggcc cgccgcctgc gcgacgggtt gggcgacgcg tcaccggtgt ccgaccggcc 53280

gacggccgtg gtggccgaca cgggtgagcc gatcgcgatc gtgtcgatgg cgtgccggta 53340

tccgggtggt gtgtcgtcgc cggaggagct gtggaggctg gtggccgacg gtgtcgacgc 53400

ggtttccgac ttcccggacg accggggctg ggacctggag ggtctgttcg acccggatcc 53460

tgatcacgcc ggtacgtcgt acacgcggca gggtggcttc ctgcgcgggg cggggctctt 53520

cgacgcgggg ttcttcggca tctcgccgcg cgaggcgatg gcgatggatc cgcagcagcg 53580

gctgctcctc gaaacgtcct gggaggccct ggaggcggcc gggatggacc cgggttcgct 53640

gaagggcacc gacgtcgggg tcttcaccgg cgtggcgagc cagggctacg gcaccggcgt 53700

ggtcgccccg gaagtggaag gcttcgcggg cacggggctc gcgggcagcg tggcgtcggg 53760

ccgggtgtcg tacgtgttcg gcttcgaggg cccggccgtg tcggtcgaca ccgcctgctc 53820

gtcctcgctc gtggcgatgc acctggccgc gcaggcgctg cggcagggcg agtgctcgat 53880

ggccctcgcc ggtggcgcga tggtcatggc cacgcccggg gccttcatgg ccttctcccg 53940

gcagcggggc ctggccgacg acgggcggtg caaggcgttc gcggacggcg cggacggcat 54000

gggcctcgcc gagggcgtcg gcgtcgtact ccttgagcgc ctctcggcgg cgcgggagca 54060

cggacaccag gtgctcgcgg tcctgcgcgg cagcgcggtg aaccaggacg gagcctccaa 54120

cggcctcacc gcccccaacg gcccgtcaca gcagcgcgtc atccgcaagg ccctcgacac 54180

ggcacgactc acccccgccg acgtggacgt cgtcgaaggc cacggcaccg gcacggcctt 54240

gggcgacccg atcgaggccc aggcgctcct ggcgacgtac ggacagggcc gggacccgca 54300

acagccgctc tggctcggct cgttgaagtc caacatcggc cacacccagg ccgccgcggg 54360

cgtggccagt gtcatcaaga tggtgcaggc gctgcgccac ggcgtcatgc cgcccaccct 54420

gcacgtgcgc gagccctcga accaggtcga ctggtcggag ggcgcggtgg aactgctgac 54480

cgaggccaag gactggccac ggaacggccg tccgcgccgg gcgggcgtgt cgtcgttcgg 54540

cgtgagcggc acgaacgccc atctgatcct ggaggaggca cccgaggccc cggtcacgga 54600

agaggcggct tcggtgggtg cggtgccgct ggtggtgtcg gcgcgcagcc agggttcgct 54660

ggcgggccag gccggtcgcc tcgcgtcgta cgtggagtcg gacgcgggtg gtgcctcact 54720

ggcggacatc gccggggcgc tggtgtcggg tcgtggggtg ttcggcgagc gtgccgtggt 54780

ggtggcgggt tcggaggacg aggcgctgtc cgggttgcgg gcgctggcgc ggggcgcgag 54840

tgatctgaat ctggtgtccg cgagcggggg tgtgccgggg aatgtggtgt gggtgttccc 54900

cgggcagggc tcgcagtggg tgggcatggg ccgtgaactc cttgacacct ccccggtgtt 54960

cgccgagcgg gtgggggagt gtgctgcgac gctggagccg ttggtggact ggtcgctcgt 55020

cgacgtacta cggggtgagg ccgagcctgc gttgttggag cgggtggatg tggtgcagcc 55080

cgcgagcttt gcggtgatgg tggggttggc ggcggtctgg gcgtctgcgg gtgtgatccc 55140

tgatgcggtg gttggtcact cgcagggtga gattgcggcg gcttgtgtgg cgggtgcgtt 55200

gtcgcttgag gatgcggtgc gggtggtggc gttgcggagt caggcgatcg cggcgcggct 55260

ggccggacgg ggcggcatgg cgtcggtggc gctgagtgag gaggaggcgg ccgggcgtct 55320

ggagccgtgg gccgagcggg tcgaggtcgc ggccgtgaac gggccttctt ccgtggtggt 55380

cgcgggtgat gcccaggcct tggacgaggt cctggaggcc ctgtccagtg acggtgtccg 55440

cacccgccgg gtggccgtgg actacgcctc ccacacacgg catgtcgagg acatccgcga 55500

caccctggcc gaagctctgg ccggggtgag cgcgaaggcc ccggtggtgc cgttctactc 55560

gaccgtcacg ggcagttggg tggaggacgc cggggtcctg gacggccggt actggtaccg 55620

caacctgcgc ggacaggtgc ggttcggccc ggccgtggcc gacctgctct cccagggcca 55680

cggtgtgttc gtggaggtca gcgcccaccc cgtactggtt cagccgatca ctgagatcgt 55740

cgacgacgcc gacggcgcgg cagtggtgac cggttcgctg cggcgtgagg aggggggcct 55800

gcggcggctg ttgatgtcga tggcggagct gttcgtgcgg ggtgtggccg tggactggtc 55860

cgcgatcgtg cccacgggga ccgcgtcggg gcgcgtggcg ctgccgacgt acgccttcga 55920

ccatcagcac tactggctca agccgggcgc acaagccacc gacgcggcgt cactgggcct 55980

tgcgggggcc gaacaccccc tgctgggtgc ggtggtgcgg ctgccgcagt ccgacggact 56040

tgtcttcacc tcacggttgt ctctgcggac ccagccgtgg ctcgccgacc atgccatcgg 56100

tggtgtggtg atcctgccgg gcacgggact ggtggagctg gcggtacggg cgggtgacga 56160

ggccgggtgc ggggtccttg aagagctggt gatcgaggcg ccgctggtcg tgcccgagcg 56220

cggcggggtg cgggtgcagg tgtcgctggg cgggccgggt gagaacggtg tgcgcacggt 56280

ggaggtgtac tcccagcgcg aggacgcggg cgatgaagcc gacgcgtgga cgcggcacgc 56340

caccggcctc ctctcggcag cggcacggcc ggacggcgta agggctggta ctggtaccgg 56400

cttcgacttc acggcctggc cgccgccggg agcgcggccc gtcgaactcg gggacctcta 56460

cggcgacctg gccgagcacg gctacgcgta cggcccggcg ttccaaggtg tgcgggcggt 56520

gtggcggcgc ggggacgagg tgttcgcgga ggtcgtgctg ccggaggagc agcgtaagga 56580

ggcgggcagg tacggcatcc acccggccct gctcgacgcg gcccttcagg cgggcacgat 56640

cggcgccgcg gcgggtccgg cggtggaggg ttccggggag ccggtgctgg cgttcgcgtg 56700

gaacggtctg gtgctgcacg ccgggggtgc ttcggcgctg cgcgtacgcg tcgcgcccag 56760

tggtccggac gcgctgtcgg tcgaggcggc ggacgaggcc ggtggcctgg tggtcacgat 56820

ggactcgctg gtgtcccggc cggtgtcggc ggaccagttg ggagcggcga cggccgccca 56880

agcgctgttc cacgtggact ggacggagct gcccccggcc caaggggcgg gttccgcgct 56940

gtcgtggatg tcggtgatcg ccgccgatga ggtggcgacg ctggcggagg aggtggcggc 57000

tggggcggcc gtgccgatgg cggcggttct ggaggccttc ggcggtgtgg gtggtgagga 57060

tgcggtactc gccctgacct ctcaggtcct tgaggtcgtc cagggatggc tggccggggc 57120

cgggcttgag gagtcgcgcc ttgtggtggt gacgcggggt gcggtgcctg caggtgaggg 57180

tggggtgacg gatccggcgg cggccgcggt gtggggtctg gtgcgtgcgg cacagtccga 57240

gcacccggac cggatcgtcc tgctggacct cgacccggcc tccgacgacg aagcgggctc 57300

cgtactggac acggtactgg gcgccgtcct cgccaccggc gagcctcaga tcgcgctccg 57360

cggcacgaca ctgtccgtgc cgcgactggc tcgcgtcgct gatcaagtac cgcagagtcc 57420

ggtggtgttc gggtcggagg gcactgtgtt ggtctcgggt ggcgggtcgc tgggtgctct 57480

ggtggctcgg catctggtcg tccggcacgg ggtgcggggc ctggtgctcg ccagtcggcg 57540

gggtgtggat gcggagggtg tgccggagct tgtcgcggag ctgaccgagc tgggtgcgac 57600

ggtgtcggct gtggcctgtg acgtctccga tcgtgatcag gtgaaggcac tgctggcggc 57660

cgtaccgcaa gagcggcgac tcacgggtgt cgtccatacg gcgggtgtct ttgacgccgg 57720

tgtgatcggg gcgctgtctc cggagcgtct cgcgggggtg ttcgcgccga aggtggatgc 57780

ggtgcggcat ctggacgagc tgactcgggg cctggatctg cgtgcgttcg ttgtttactc 57840

gtcggcttcg tctgtgttca tgggtgcggg tagtggtggt tatgcggcgg cgaacgcgtt 57900

cctggacggg ttgatggcgc agcggcgggc ggcgggtctg cccggtctct cgctcgcctg 57960

gggcccgtgg gagcagacca ccggcatggc ggacaccatc gacgacctca ccagggcgcg 58020

gatgagccgg cgtgaagggc gcgggggagt cctggcgctc aagtccagcg aaggcctgga 58080

gctgttcgac gccgccctgg cgtccgggtg ggcgctgctg gtgccggtca agctcgacct 58140

gcgtgatgcg cgtgcggacg cggtggccgg cggcggggta ccgcccctgc tgcgcggtct 58200

cgtccgcgcg gcccggcaac cggcacgggc ggcgggaggc accggcggcg atcaccggca 58260

gttggccgaa cggctggccg ggctcgccgc cgcggagcgt aggaaattcc tgctcgacct 58320

ggtgcgcgcc caggccgcgg tggtgctcgg ccacaccggg ccggggagcg tacggccgga 58380

cacggcgttc aacgaagccg ggttcgactc gctcacggcg gtggagctgc gcaaccggct 58440

gcgcgaggcg accggcctga agctgcccgc caccctggtc ttcgaccatc cgaccccggt 58500

cgccctgtcg gcgtacctgc tcggcgaaat cgccgtcgac agcgcgtccc ccgcggaccc 58560

ggtcctcgcc gggctcgccg gactggaggc ggccatcgag tcggcggcgg gcgacgagga 58620

ggcccgggac cggatcaccg tacgcctgcg ggaactgctg aaggccgccg aggccgccga 58680

ggccgctgag gcggcgggag gccgggacgc ggcaccggac gccgcgggca cgagcgaaga 58740

cgatctcgaa aacgcgagcg acgaggagct gttcgcgatc ttcgaccggc tcgactgacc 58800

agaccccgcc cgactgagca ggacccgccc cgattgacca ggccccgccc gactgactag 58860

gacccgcccc gactgagcag ggcccgtaag gcgcgtcgac cgacccgcat ccactccacc 58920

gccatcgcac acatcgaatt cctgggagct gagttcagtg gctgacgagg tacaactccg 58980

cgactacctc aagagggcca tcgccgacgc ccgcgacgcc cgcaagcgtc tgcgtgaggt 59040

cgaggaccag gcgcgggagc cgatcgcgat cgtgtcgatg gcgtgccggt atccgggtgg 59100

tgtgtcgtcg ccggaggagc tgtggaagct ggtggccgac ggcgtcgacg cggtttccga 59160

cttcccggac gaccggggct gggacctgga gggtctgttc gacccggatc ctgatcacgc 59220

cggtacgtcg tacacgcggc agggcggctt cctgcgcggg gcggggctct tcgacgcggg 59280

gttcttcggc atctcgccgc gcgaggcgat ggcgatggat ccgcagcagc ggctgctcct 59340

ggaaacctcg tgggaggccc tggagcatgc gggcatcgat cctctgtcgt tgaagggcac 59400

cgacgtcggg gtgttctccg gcgtgtccag ccaggggtac ggagccggtg ccggggtggt 59460

gacgccggag tcggagggct tcgcgggcac gggcgcgacg ccgagcgtgg cctcgggccg 59520

ggtgtcgtac gtgttcggct tcgagggccc ggcggtcacc atcgacacgg cgtgctcgtc 59580

gtcgctcgtg gcgatccacc tggccgcgca ggccttgcgg aacggcgact gttcgatggc 59640

cctggccggt ggtgcgatgg tcatggccac gcccggcaac ttcgtggcgt tctcccggca 59700

gcggggcctg gccacggacg ggcggtgcaa ggcgttcgcg gacggcgccg acggcatggg 59760

cctcgccgag ggcgcgggcg tggtcctgct cgaacgcctc tcggtggccc gggaacgcgg 59820

gcatcaggtc ctcgcggtgc tccggggcag cgcggtgaac caggacggcg cctccaacgg 59880

cctcaccgcc cccaacggcc cctcccaaca gcgggtgatc cgccgggccc tggcgaacgc 59940

ggacctgtcc ccgtccgacg tggacgtggt ggagggccac ggcacgggga cgtccctcgg 60000

cgatccgatc gaggcccagg cgctcctggc gacgtacggg aaggggcggg acccacagca 60060

gccgctctgg ctcggctcgt tgaagtcgaa catcgggcac acccaggccg ccgccggtgt 60120

gggcagcgtg atcaagatgg tgcaggcgct gcgccacggc gtcatgccgc cgaccctgca 60180

cgtccaggag cccacgactg aagtggactg gtcggcgggc gcggtcgagc tcctgacgga 60240

ggcgcgcgcg tggcctcgtg acgctcgccc gcgccgggcc ggggtgtcct cgttcggcgt 60300

gagcgggacg aacgcgcatc tgatcctgga ggaggtcccc gaggagccgg tctccgagga 60360

gccggtcccc gaggagagcg ggtcgtcggg tgtggtgccg ctggtcgtgt cggcgcgtgg 60420

ccacgcggcg ctctcgggac aggccgcgcg cctcgcggca cgcgtcgaaa ccgtcggtga 60480

ggtgtcgttg gcgggtgtgg ccggggcgct ggtgtcgggc cgggcgctgc tggacgaccg 60540

cgcggtggtc gtggcgggct cggccgagga cgcactcgcc gggctggggg cgctggcgcg 60600

gggcgagagc ggcccggacg tcgtgtccgg gagcgtcgcc ggttcaggtg agccgggcgg 60660

cctcgtgctg gtgtttccgg ggcagggttc gcagtgggcg gggatggggc gtgaactgct 60720

cgattcctcc ccggtgttcg ctgagcgggt cgctgagtgt gcggctgccc ttgagccgtg 60780

ggtggactgg tcgcttgtcg acgtactgcg gggtgaggtc gagcctgcgt tgttggagcg 60840

ggtggatgtg gtgcagcccg cgagctttgc ggtgatggtc gggctggctt ccgtgtgggc 60900

gtccgtgggt gtgaagccgg atgcggtgct cggtcactcg cagggtgaga tcgcggcggc 60960

ttgtgtggct ggtgtgttgt cgcttgagga tgcggtgcgg gtggtggcgt tgcggagtca 61020

ggcgatcgcg gcgcggctgg cggggcgtgg gggcatggcg tcggtcgccc taggtccgca 61080

ggaagtggcc gggcgtctgg agccgtgggc cgagagggtc gaggtcgcgg ccgtgaacgg 61140

gccgtcctcc gtggtgatcg ccggtgacgc cgaggccttg gacgaggccc tgcgggagct 61200

ctccggtgac ggcgtacgag tgcggcgcgt ggccgtggac tacgcctcgc acacccgcca 61260

cgtcgaagat atccgggaca ccctcgccga ggcgctgacc ggggtgagcg cgcaggctcc 61320

ggccgtgccg ttctactcga ccgtctccgg cggttgggtg caggaggccg gtgtcctcga 61380

cagtgagtac tggtaccgca acctgcgcgg gcaggtgggc ttcggcccgg ccgtggccga 61440

tctgctggcg caggggcacg gcgtgttcgt ggaggtcagc gcccaccccg tactggtcca 61500

gcccgtcagc gagatcgtgg acgagagcgg tgccgacgcc gtggtgacgg gctcgctgcg 61560

ccgcgacgaa gggggcttgc ggcggctgct gatgtcgatg gcggagctgt tcgtgcgggg 61620

tgtggccgtg gactggtccg cgatcgtgcc cacggggacc gcgtcggggc gcgtggcgct 61680

gccgacgtac gccttcgacc atcagcacta ctggctcaag ccgggggagt cggcatccga 61740

cgcggcgtcg ctgggccaga ctcccgccga acatccgctg ttgggcgcgg tggtgcggct 61800

gccgcactcc gacagccttg tcttcacgtc ccggctctcg ctgcgctcgc acccctggct 61860

ggccgatcac gccgtcggtg gtgtggtgat cctgccgggc acagggctcg tggagttggc 61920

ggtacgggcg ggtgacgagg ccgggtgcgg ggtccttgaa gagctggtga tcgaggcgcc 61980

actggtcgtg gccgagcatg gtggggtccg tatgcagatc gcggtgggcg caccgggtga 62040

gagcggtgcg cgcacggtcg aggtgtactc ccagcgcgcg gacgccggcc ctgccgacgc 62100

atggacgcgg cacgccaccg gactcctgac ggcatcgcaa gcgacacccg cggaggtgcg 62160

ggagtacgac ttcgccgcct ggccgccgcc cggcgcgcgg aaggtcgagg tggaggcgag 62220

cgacttctac gacggtctgg tcgcgcgtgg tttcggctac gggcccgcgt tccagggggt 62280

gcgggccgcg tggcggcgcg gcgacgaggt gttcgcggag gtcgcgctgc ccgaggagca 62340

gcgcaaggag gcgggcaggt acggcatcca ccccgcgctg ctcgacgcgg ccctccaggc 62400

cgggacgttc gccgccgagg cggatccgac ggcggaggac tccggggaac cgatcctcgc 62460

gttcgcgtgg aacggcctgg tgctgcacgc cgcgggagct tcggcgctgc gcgtacgcat 62520

cgctccgtgc gggccggacg cactggccgt acaggctgcc gacgagaccg gtggcctggt 62580

cgtcacgatg gactccctgg tgtcccggcc ggtgtcggcg gaccagttgg gggcggcgac 62640

ggacggggcg gtcgcagact cgctgtacca cgtggactgg accgagctgc ccccggccca 62700

ggtgcgggag cccgcgccgc catgggcggc cgtggccacc gcggccgagg tggcggccct 62760

cgcagggagc ccgacggttc cgccggtggc cgtcctggaa gcggtcggag acgatggcgc 62820

ggacgcggta ctggccctga cctcccgggt gttggcggtg gtccaggcgt ggctcgccga 62880

ggccgggctt gaggagtcac gccttgtggt ggtgacgcgg ggcgcggtgt ctgcgggtga 62940

ggacgcggtg ctggatcccg cgggggccgc cgtgtggggt ctggtgcggg ccgcgcaggc 63000

cgagaacccg gaccggatcg tcctcctcga caccgacccg gtatcccagg agggcctggc 63060

atccctactg ggaccggccc ttgccaccgg cgaaccccaa gtggccgtgc gtggagcgac 63120

tctctccgtg ccgaggctgg atcgcgtcac tgatcaagta ccgggaaatc cggtggtgtt 63180

cgggtcggag ggcactgtgt tggtctcggg tggcgggtcg ctgggcgctc tggtggctcg 63240

gcatctggtc gtccggcacg gggtgcgggg cctggtgctc gccagtcggc ggggtgtgga 63300

tgcggagggt gtgccggagc ttgtcgcgga gctgaccgag ctgggtgcga cggtgtcggc 63360

tgtggcctgt gatgtgtcgg atcgtgatca ggtgaaggcg ttgctggcgg ccgtagcgga 63420

cgatcacgag cggcgactca cgggtgttgt tcatacggcg ggtgtcttcg atgccggtgt 63480

gatcggggcg ctgtctccgg agcgtctcgc gggggtgttc gcgccgaagg tggatgcggt 63540

gcggcatctg gacgagctga cgcggggcct gggtctgtgt gcgttcgtcg tctattcgtc 63600

ggcttcgtct gtgttcatgg gtgcgggcag tggtggttat gcggcggcga acgcgttcct 63660

ggacgggttg atggcccagc ggcgggcggc gggtctgccc ggcctctcgc tcgcatgggg 63720

cacctgggag cacgccacca acatgaccac gcacctcagc acggccgacc ggacgaggat 63780

gagccggcgc gcgaaccgta ccggcgtcgt gggcatcaag cccgccgagg gcatggagct 63840

gttcgacgcc gccgtggcgt ccgggcgggc actgctggtg cccgtcaagc tcgacctcca 63900

gggcgtgcgc gccggtgcgg cgggcggcgg tggagtgccg cccctgctgc gcggcctcgt 63960

gcccgcggcc cggcagcagg cgcgggcggc ggccgcgaac gaaggcgacc tcgtccgccg 64020

cctggcccag ctcaccggcc cggagcagga ggccctgctg ctcgacctgg tgcggacaca 64080

ggccgccgtg gtgctcgggc acagcgggcc gggcggcgta cgggcggaca cggcgttcaa 64140

ggacgtcggc ttcgactcgc tcacctcggt ggagctgcgc aaccggctgc gcgaggcgac 64200

cggcctgaag ctgcccgcca cgctcgtctt cgaccagccg aacccgctgg tcctcgcccg 64260

ccatctgcgc gacggcctcg gcgtcagcga cgacgtgctc gcccgcgtgc acgcgaggat 64320

cgaggacgtc gagacgctga tcggcggact gtccctcgac gcggccatga aatccagtgt 64380

cgcgctgcgc cttcagggac tggtggccag gtgcaacgga gtgctcgagc agacggacgt 64440

ctcgacggtc gcggacaagc tggaatccgc gtcggccgac gaagtcctcg acttcatcga 64500

cgacgaactg ggtcttgtct gagaccggtt cgaggccctg cggcgccacc cgaccttcgt 64560

ggaccgaaca acccttcgtg aggactgaac catggccacg gacgacaaac tcctcaagta 64620

cctcaagcgc gtcacgacgg aactgcacca gctgcggaaa cagggatcgg cgcacccggg 64680

cgagccggtg gcgatcgtgg ggatggcctg ccggctgccc ggcggcgtga cgggcccgga 64740

ggacctgtgg cgcctggtgc gcgagggccg ggacgcggtg tcgggcttcc ccgacgaccg 64800

gggctgggaa ctggagggcc tcttcgaccc tgaccccgac aacccgggta cgtcctacac 64860

cgaccagggc ggattcctgc gcggggcagg cctcttcgac gccgggttct tcggcatctc 64920

gccgcgcgag gccctggcca tggacccgca gcagcggctg ctcctggaaa cctcctggga 64980

ggcgctggag catgcgggcg tcgaccccct gtcgttgaag ggcaccgacg tcggagtgtt 65040

ctccggcgtg tccagccagg ggtacggggc cggtgccggg gtggtcgccc ccgagttgga 65100

gagcttcacc ggcacgggcg tggccgggag cgtggcgtcg ggccgggtgt cgtacgtgtt 65160

cggcttcgag ggcccggcgg tcaccgtcga cacggcgtgc tcgtcgtcgc tcgtggcgat 65220

ccatctggcc gcgcaggcac tgcggcaggg cgagtgctcg atggccctgg ccggtggcgc 65280

gatggtcatg tcgacacccg gcaccttcgt ggcgttctcc cggcagcgcg gcatggcatc 65340

cgacgggcgg tgcaaggcgt acgccgacgg cgccgacggc atgggcctcg ccgagggcgc 65400

gggcgtggtc gtactcgaac gcctctcggt cgcgcaggag cgcggccacc gcgtcctcgc 65460

ggtcgtccgg ggcagcgcgg tgaaccagga cggcgcctcc aacggcctca ccgcccccaa 65520

cggcccctcg caggaacgcg tgatccgcaa ggccctggcc ggggccggac tggtctcggc 65580

ggacgtcgac gtcgtggagg gccacggcac cggcacggcc ctcggcgacc cgatcgaggc 65640

ccaggcgctc ctggcgacgt acggacaggg ccggagcccc cgacagccca tatggctggg 65700

ctcgttgaag tccaacatcg ggcacaccca ggccgccgcc ggtgtggcca gcgtgatcaa 65760

gatggtgcag gcgctgcgcc acggcgtcat gccgcccacc ctgcacgtcc aggagcccac 65820

gaccgaggtg gactggtcgg cgggcgcggt cgagctcctg acggaggcgc gcgcgtggcc 65880

tcgtgccaca cgtccgcgcc gggccgggat ctcctcgttc gggatcagcg gcaccaacgc 65940

ccatctgatc ctggaggaag cgcccgccga cgaggccgag cccgccccgg cggaggcgga 66000

ggtggaggcg gagcccgggg gcgtggtgcc gctggtcgtc tcggcgagca gtggcggcgg 66060

gctgacgggc caggccgcgc gcctgacggc gtatctggag gccccgggaa cgtccctggc 66120

gggtgtcgcc ggggcactcc tgtcggaccg ggccctgctc gacgaacgcg ccgtggtgct 66180

cgcccgcacg gacaccgagg ccctggcggg cctcggcgcg ctcgcacgcg gcgagagcgc 66240

caccggtgtg gtgtccggcc ggatcgccgg gtccggcgtg ccgggcaagg tcgtgtgggt 66300

gttccccggg cagggctcac agcgagtggg ggcgggccgg gaactgtacg accggcaccc 66360

gctgttcgcg gcggccttcg acgaggcctg cgagcacctg gacgcgcgcc tcgccggatg 66420

ggtcgaccat ccggtgcgtg acgtggtcct gggcacggtg gcgggcagcg ccgacctgct 66480

gaaccagacg gtgttcaccc aggccggact gttcgcggtg gagagcgcgc tggcccgcct 66540

cgtggagtcc tggggcgtac gcccggacgt ggtcatcggg cactcgatcg gcgaggtcac 66600

cgccgcgtac gtcgcgggcg cgctgacgct gcccgacgcg gcgcgcctgg tcgccgccag 66660

ggggcggctc atgcaggcgc tgccccctgg cggggccatg gtcgcggtgg ccgcgaccga 66720

ggagcaggtc accgggctgc tgggcgcggg cgtggagatc gcggcggtca acggcccgtc 66780

cgcgctggtg ctctccggag acgaggacgc ggtcctcgcg gcggcggacc agctgcggga 66840

gcgggggcac aaggcgaagc gcctcgccgt ctcgcacgcg ttccactccg cgcggatgga 66900

gccgatgctc gccgacttca ccgccgagct cgccacggtg gcgtggcggc agcccgcgat 66960

cccggtgatc tcgaacgtga ccgggcggcc cgcagagccc gacgcactcg ccgacccccg 67020

gtactgggcc gagcacgtgc ggcgtccggt gcggttcgcc gaaggcatcg cggccgccgt 67080

ggagtgcggc ggcaccctgt tcgtggaact gggtcctggc gcggccctga ccggcctggt 67140

cgaggagacc gccaccgcct cgaccgccgc ggtgacctgc gtcgcggccc tgcgcgaagg 67200

acgcccggag gagcggacgc tcctcgcctc gacggccgaa ctgttcgtgc ggggcgtgac 67260

cgtggactgg acccgcctac tgcccgccgg agccgcctcc gcccccgtgg acctgccgac 67320

gtatgccttc gaccaccggc actactggct gcagcccggc gcatcggcca cggacgccgt 67380

gtcgctgggg cagaccccgg cggaccaccc gctgctgggc gcggtggtgc ggctgccgca 67440

ctccgacggc ctggtcttca cctcccggct ctcgctgcgc tcgcacccgt ggctggccga 67500

ccacgcggtc ggcggcgcgg tcctcgtccc cgggaccgcg ctcgtggaac tcgcggtgcg 67560

cgccggagac gaagccgggt gcgcggccct cgaagagctc gtggtcgagg cgccgctggt 67620

ggtgcccgcg cgcggcgcgg tgcgggtgca ggtcgcggtg ggcgccccgg gtgcgacggg 67680

cgcgaagggc tcgcgcacgg tggaggtgta ctcccagccc gaggacgccg ccgacggggg 67740

cggcaccgac acgtggaccc ggcacgcggc gggcctcctg tcggtcaccg tcccgccccg 67800

cggcgacagc gccttcgact tcaccgcctg gccgccgcgcggcgcgcagc cgatcgacgt 67860

ccaccccgat gacttctacg acggactgac cgagcgcggc tacggctacg gccccgcgtt 67920

ccgcggcctg cgggcggtgt ggcggcgcgg cgaggaactc ttcgcggagg tcgccctgcc 67980

cgatgaacag cgcaaggacg ccgagaggtt cggcatccac cccgcgctcc tcgacgcggc 68040

cctgcacgcc ggaatgctcg ccaccacggc ggacacgaca gcggatgacc ccgggcagcc 68100

ggtgctgccg ttcgcgtgga acggcctcgt gctgcacgcc gccggtgcct ccacgctccg 68160

ggtacgcctg acgcccggcg gccccggcgc cgtgtccctg acggcggcgg acgagaccgg 68220

cggcctcgtc gtggccatgg actccctggt gtcccggccg gtgtccgcag gccagttgga 68280

gagcgcgacg gacacggcgg ccgccgacgc gctcttcgcc gtggagtgga ccgagctgcc 68340

gtcggtacgg ggggacgcgc cgtcctgggt gccggtggcc acggccgacg acgtgatggc 68400

cctcgaccgg agcgcgacgg ccccgccgtt ggccgtcctg gaagctttcg gcggcgaagg 68460

ggagggcgcg gcactcgccc tgacctccca ggtccttgag gtcgtacagg cgtggctgac 68520

cggggccggg tttggggatt cgcgccttgt ggtggtgacc cggggggcgg tgcccgcggg 68580

cgatggagcg gtgacggatc cggctggagc ggcggtgtgg ggtctggtgc gtgccgcgca 68640

ggcggagaat ccggaccgga tcctcctcat tgataccgat cccacggccg aaggggatct 68700

ggatgcggcg ctgggggtgg cgctggccag tggtgagccg caggttgcgg tgcgtggtgc 68760

gactttctcg gtgcccaggt tggtccgtgt tgcgggtcgg gggctgggtt cggggaacgt 68820

attcgccgct ggaaagaccg ttctacttag tggtggaaca ggatccctgg gtggcctggt 68880

ggcccgccac ttggtcacgc ggcacggtgt gcggcatctg ttgcttgtga gtcgtcgggg 68940

tgcggatgcg gagggcgtgc cggagttggt ggccgagctc gccgagcagg gcgcggtcgt 69000

gtcggtagtg gcctgcgacg tgtccgaccg cgatcaggtc cgggccctgc tggccgccgt 69060

accggacgag caccctctga gcggcgtcgt ccacaccgcg ggggtgctgg acgacggagt 69120

gatcggagcg ctgacgccgg agcgcctcgc gggcgtgttc gcgccgaagg tggacgcggt 69180

ccgccacctc gacgagctga cccgcgaact gggcctggaa ctggacgcgt tcatcgtcta 69240

ctcctccgcc tcgggagtgt tcggttcggc aggtcagggc aactacgcgg cggcgaacgc 69300

ctacctcgac gggttgatgg cgaaccggcg ggcggcgggc ctgcccggta tctcgctggc 69360

ctggggcctg tgggagcaga ccgcgggcat gaccgcacac ctggacgagg tggaccaggc 69420

gcgcgccagc cgtggcggcg tgctggccat cacgcccgcc gaaggcatgg agctgttcga 69480

cgccgcggcg gcgtcccggc acgcgctgct ggtcccgatc aagctggacc tgcggggagc 69540

gcgcgccggt gcggcctccg gcggagcggt tccccacctg ctgcgcggcc tcgtacggac 69600

gggccggcag caggcgcggg cggcgtccgg gagcgacggc gagctggccc gtcgcctcgc 69660

cggactcacc gaggcggagc agaaggccct gctcctcgac ctggtccgca cccaggccgc 69720

cgtcgtgctc gggcacgcag gccccggaag cgtgcggccg gacatggcct tcaacgaagc 69780

cgggttcgac tcgctcacct cggtggaact gcgcaaccgg ctgcgtgagg cgaccgctct 69840

caagttcccc gccaccctcg tcttcgacta cccgaccccg gaagcgctcg cccgctacct 69900

gcgcgaagag ctcgccgatt cggccgaagc cgccgcaccg gccgcggctg tcgtcgcgga 69960

cccggacgag ccgatcgcga tcgtcgggat ggcgtgccga ctgcccggcg gggtgacgga 70020

ccccgacggc ctgtggcggc tggtgcgcga gggccgtgaa ggcgtgtcgc cgttccctga 70080

ggaccgcggc tgggacctgg agggcttgtt tgacgcggat ccggacaacg ccggtacgtc 70140

gtacgccagt cagggcggtt tcctgcacgg cgcgggcctc ttcgaccccg ggttcttcgg 70200

catctcaccg cgcgaggcgc aggcgatgga cccgcagcag cggctgctcc tcgaaacctc 70260

atgggaagcc ctggagcagg cgggcatcga cccgctgtcg ctgaagggca ccgacgtcgg 70320

ggtgttctcc ggcgtgtcca cccagggcta tgggatgggc gcggccgcac gggacgtgga 70380

gggctacacg ggcacgggtt cggcgcccag cgtggcgtcg ggccgggtgt cgtacgcctt 70440

cggcttcgag ggcccggcgg tcaccgtgga cacggcgtgc tcctcctcct tggtcgcgat 70500

gcacctggcc gcgcaggcgc tgcggcaggg cgagtgctcg atggcgctcg ccggaggcgt 70560

cgcggtgatg gcgacgcccg gggccttcgt ggagttctcg cggcagcggg gcctggccac 70620

cgacgggcgc tgcaagtcct acgccgcggg agccgacggc accggctggt ccgagggcgt 70680

cggcctggtg gtcctcgagc gcctctcggc ggcgcgggag cgcggccacc gggtcctggc 70740

cgtgctgcgc ggcagcgcgg tgaaccagga cggcgcctcc aacggcctga ccgcccccaa 70800

cggcccctcc cagcagcggg tgatccgccg agccctcgcg aacgcgggtc tctccccgtc 70860

cgatgtggac gtggtggagg gccacgggac cggcacaacc ctgggcgacc cgatcgaggc 70920

ccaggcgctg ctcgcgacgt acgggcaggg gcgggatccc gaacagccgc tgtggctggg 70980

ctcgttgaag tccaacatcg gtcacgcgca ggcggccgcg ggtgcggccg gtgtgatcaa 71040

gatggtgcag gcactgcggc acgcgttcatgccccccaca ctgcacgtcg acgagccgac 71100

ggccgaggtg gactggtcgg cgggcgcggt ggagctgctg accgaggcgc gggagtggcc 71160

gcacaacggt cgtccgcgcc gggccggggt gtcctcgttc gggatcagtg gcacgaatgc 71220

gcatctgatc ctggaggagt cgcctgagga gccggtttcg gaggagagcg ggccggtggg 71280

tgtggtgccg ctcgtggtgt cggcgcgtag tcgggcttcg ctgacgggac aggccggccg 71340

gctggtgtcg tacgtggggt cgggggctgg tggttcgtcg cttgtggggg tggccggggc 71400

gttggtgtcg ggccgggccg tactgggtga gcgtgcggtg gtggtggcgg gttcggaggg 71460

cgaggcggtg tccgggttgc gggcgctggc gcggggcgag agtgatgtga atctggtgtc 71520

cgggggcggg ggtgtgccgg ggaaggtggt gtgggtgttc ccggggcagg gttcgcagtg 71580

ggcgggcatg gggcgtgaac ttctcgactc ctcgccggtg ttcgctgagc gggtggggga 71640

gtgtgctgcg gcgctggggc cgttggtgga ctggtcgctc gtcgacgtcc tgcggggtga 71700

ggtcgagccc gcgctgcttg agcgggtgga tgtggtgcag cccgcgagct ttgcggtgat 71760

ggtggggctg gcttcggtgt gggcgtccgt gggtgtgaag ccggatgcgg tgcttggtca 71820

ctcgcagggt gagatcgcgg cggcttgtgt ggcgggtgcg ctctcccttg aggatgcggc 71880

gcgggtggtg gcgttgcgga gtcaggcgat cgcggcgcgg ctggccggac ggggtggcat 71940

ggcgtccgtc gccctcgcag agcaggaagt ggccgggcgg ctggagccgt gggccgagcg 72000

ggtcgaggtc gcggccgtga acgggccttc ctccgtggtg attgctggtg atgccgaagc 72060

cttggacgag gtcttgcaag tgctctccgg tgacggcgta cgagtgcggc gcgtggctgt 72120

ggactacgcc tcccacacac ggcacgtcga ggacatcgag gacaccctcg ccgaagccct 72180

ggccggggtg agcgcgcagg ctccggccgt gccgttctac tcgaccgtca cgggcagttg 72240

ggtggaggac gcgggggtgt tggacggcgg gtactggtac cgcaacctgc gcgggcaggt 72300

gggcttcggc ccggccgtgg ccgatctgct ggcgcagggc cacggcgtgt tcgtggaggt 72360

cagcgcccac cccgtactgg ttcagccgat cactgagatc gtcgacgacg ccgacagtac 72420

ggcggtggtg accggttcgc tgcggcgtga ggagggtggt ccgcggcggc tgttggcgtc 72480

ggtggccgag ctgttcgtgc ggggcgtgac cgtggactgg gcccgcctgc tgcccgccgg 72540

agccgcctcc acacgcgcgg acctgccgac gtacgccttc gaccaccggc actactggct 72600

caagccggcc gcgcaaggca ccgacgcggc ggcactgggc cttgcggggg ccgaacaccc 72660

cttgctgggt gcggtggtgc ggctgccgca gacggacggg ctcgtcttca cctcgcgcct 72720

gtcgctgcgg acccacccct ggctggccga ccacgcggtc agcggagtcg tactcgtccc 72780

gggcaccggg ctcgtggaac tggccgtgcg ggccggtgac gaggccgggt gcggggtcct 72840

ggaagagctg gtgatcgagg cgccgctggt cgtgcccgag cacggtgggg tgcgggtgca 72900

ggtcgcggtg ggcgggcccg gcgagacggg cgcgcggacc gtggaggtgt tctcccagcg 72960

cgaggacgca gccgacgacg gtggcgcgga cgcgtggacg cggcacgcca ccggactcct 73020

cgcgaccacc ggccgacccg gggccggcgg cagcggctac gacttcgcgg cctggccgcc 73080

gcccggcgcc gaaccggtgg acatcagcga cggctacggc ctgctcgccc ggggcggcta 73140

cggctacggg cccgcgttcc agggcgtacg ggccgtgtgg cggcgcggcg acgaagtgtt 73200

cgcggaggtc gccctgcccg aggagcagcg cgaggaggcg ggcaggtacg gcatccaccc 73260

cgcgctcctc gacgcggccc tgcactcgac catgctgaac gccgtggcgg cgctcacggc 73320

ggccgccgac ggcgcccctg accgcgagga cggccttgag ctgcggctgc cgttcgcctg 73380

gaacgggctc gaactgcacg cggcaggcgc atcggcgctg cgcgttcgcg tggcgaggcc 73440

cgagcccgac gcgctgtccc tggaagcggt ggacgaggcc ggtggcccgg tcgtgacgat 73500

ggaatcgctg gtgggccggg tggtgtccac cgagcaactg gcggcggcgt cggacacggc 73560

acgcgtcgac tcgctgtacc gggtggagtg gaccgagctg cccccggcgc gggaggcggg 73620

acccgcgcct tcacgggtga cggtagccac cgcggaggaa gtggcgacgc tggccgacga 73680

cgtggagtcg ggagcggcgg cggccccggc agcggcggtg ctggacgccg tcggcggcga 73740

cgaggaggac gcggcgcttt ccttggcctc ccgggtcctc gacgtggtgc agtgctggct 73800

ggccgggacc gggctcgaag agtcgcggct cctcgtggtg acgcggggcg cggtccccgc 73860

gggcgacgga gcggtgacgg atccggccgg agcggccgtg tggggcctgg tacgcgccgc 73920

acaggcggag aacccggacc ggatcgtcct gctcgacacc gacaccgaca ccgacaccga 73980

cgcaggcacc gacaccgacg caggcaccga caccgacacc ggcatcgact tcgacgccga 74040

cacggaacgt gttctgggta cggtcctcgc cagcggcgag ccgcagctgg cggtgcgcgg 74100

cacgcgcttc tccgtgcccc gcctcacccg agccaccggc caagtcccgg acgtacccgc 74160

ggtgttcggg cccgacagca ccgtcctgat ctcgggcgcc ggatcgctgg gagctctcgc 74220

ggcccggcac ctggtcaccc ggcacggcgt acggcggctg ctcctcgcca gcaggcgggg 74280

ccgggacgcc gagggcgtgc cggagctggt ggccgagctg accgagcagg gcgcgacggt 74340

gtcggccgtg gcctgtgacg tgtccgaccg ggaccaggtc aaggccctgc tggcgtcggt 74400

accggacgag cagcggctca ccgggatcat ccacacggca ggtgtgacgg acgacggcgt 74460

gatcggagcg ctgacgccgg agcgcctgga gcgggtgttc gcgccgaagg tggacgcggt 74520

gcggcatctc gacgagctga cgcgtgaact gggcctggac ctggacgcgt tcgtcgtcta 74580

ctcgtccgtg tccggcgtct tcatgggcgc gggcagcggc agctacgccg ccgccaacgc 74640

ctacctggac ggcctgatgg ccaaccggcg ggcggcgggc ctgccggggc tctcgctcgc 74700

ctggggcctg tgggagcaga acaccggcat ggccgcccac acggacgacc tcaccaagag 74760

ccggatgagt cggcgcggcg gcctgacggc gatgacaccc accgagggca tggagctgtt 74820

cgacgccgcg gtggcgtccg gacaggcgct cctggtaccg gccaggctcg acctgcgcgg 74880

cgtacgcgcc ggtgcggcgg ccggtggcgg cgtgccgcac ctgctgcgcg gcctcgtccg 74940

cgccggccgc caactggcgc acacggcggg cggcgcgggc gagcaccggc agctggccga 75000

gcggctcgcc gcactgcccg ccgccgagcg ggcgaaggcg ctgctcgacc tggtgcgggc 75060

gcaggtggcc gcggtcctcg gccacggcac gacgtaccac atcgacgccg accaggggct 75120

cttcgagatc ggcttcgact ccctcacggc catcgagctc cgcaaccggc tgcgcgccct 75180

caccgagcgg agactcgcgc ccagcctcgt cttcgaccac ccgacgccag ggatgctcgc 75240

cgcgcacctg cacgcgctga tgtacggcgc acaggccgcg gccccggtgc cgctctccgt 75300

gtgaacccag tgatccccct gtgaatccgt agaaagcaga aagaggcgac gacgtgttcg 75360

acgtgagcga ctacctccag cggatcggct gcggcggaga gaccggcgtg gacgccgcga 75420

cgctgcggaa gctgcacaag cgccacctca tggcgattcc gtacaacggc gccgcgcagg 75480

acctccgcga cggcatccgc ctcgtggacc tcgacgagga cgccgtgttc gagacgagcg 75540

tcgtcgacgg gcacggcggc acctgcttcc agctcgcccg gctcttccac cggctgctcg 75600

ccgcactcgg ctacgacgtc acgctgatgg cgggcagcac cgccgaaggc cgcgagaact 75660

tcgggctcga cgtggagcac atgttcagcc gggtcgccct cgacggcgag gagtggctcg 75720

tcgacgtcgg ctacccgggc ccgaactaca tcgagccgct gcggatcacc gacgcggtgc 75780

agagccagta cgggtgccag taccgcctcg tcgaggacgc ctcggggttc gccctccagc 75840

ggcgggggcg ggtcagccgg tggagcgtcg tctacacctt caccaggaag ccccggcagt 75900

ggagcgactg gaaggaactg gaggagtacc tcaaccggga gtacgcggcc gcccccgccg 75960

aatccgacgc ctgcccgatc gcgcgcggca cggaccgcat cttcggggag atcgaggcgg 76020

ccaccggcgc gcacgagggc caggacgtgc tgtgcggccg caccttcgac aacggccagg 76080

tcgtgctgaa gggacgccgc tacctgacgg tccgcgacgg ccgcgaagaa gtccgcacga 76140

tcaaggacga cgacgaacac cgcgacctgg tcttctgcat cctgtccggc gacttcagct 76200

gaacgcgcgc cgcgcacacc ccgcacagca cccgtccgaa gggggacatg atggccaccg 76260

ttccgctgcg tctcgacgac cacgcgtacg acgtgctgat cggcccgggg gtgcgcgcgt 76320

cgctcaccga cgtggtccgg cgcctcggcg ccggacgggc cgtcgtcgtg tcggcccgcc 76380

ccgcggagtg ggtgcccgac accggtgtcg agaccctgct gctgcgggcc cgggacggcg 76440

agcgggacaa gaccctcgcg accgtggagg cgctgtgcgg tgagttcgtg cggttcgggc 76500

tcaccaggtc cgacgtcgtc gtgtcctgcg gcggcgggac gaccaccgac gtggtcggcc 76560

tcgcggccgc gctgtaccac cggggcgtgc ccgtggtcca tctgcccacg tcgctgctcg 76620

cccaggtgga cgccagcgtc ggcggcaaga cggcggtgaa cctgtcggcg gggaagaacc 76680

tcgtcggcgc gtactggcag cccagcgcgg tgctgtgcga cacggactac ctcacgaccc 76740

tgccgcgcag ggaactcctc aacgggctcg gggagatcgc ccgctgccac ttcatcggcg 76800

cggccgacct gcgcggcctg ccgctcgtgg aacagatcac ggcgagcgtg accctcaagg 76860

cccgcatcgt ggaggcggac gagcgggaca ccggcgtgcg gcacatcctc aactacggcc 76920

acacgctggg ccacgccctg gagaaggcga ccgactacgc cctgcgccac ggcgaggcgg 76980

tggccgtcgg cacggtcttc gcggggcgcc tcgccggagc cctgggccgc atcggcccgg 77040

cacgggtcgc ggaacaccag gacgtcgtcg cgtactacgg actgcccgcc gcgctgcccg 77100

ccgaggccga gcccgcggac ctgctgcggc tcatgcggca cgacaagaag gcgatcaccg 77160

gcctcgcgtt cgccctggac ggcccgcacg gcgcggagct ggtgagcgac gtgccgcccg 77220

aggtggtcgc gcgcgtcctg gagcggatgc cccgggcgcc cctcgccgac ctcgtcggcg 77280

ccgccgcgac cggcgccgcc gcgaacgacg cggcccggac gtgaggcgcg cgccccggcc 77340

gacggccgga cccgagagcg cggccggacc cgaagggacg gccgacgccg ccgatccggt 77400

gcaccgcctc ctggcgggga agctgacggc ggccctggcc cgaccggccg cccagcagcc 77460

gcactggccg gacccggagc gggcgaaggc cgtggtggcg tccctgagcc gggccgcgcc 77520

gatcgtgacg cccgccgaga ccgcgcgcct ggcggaccgg ctcgccgcgg tcgcgcgcgg 77580

cgaggcgttc ctgctccagg gcggtgactg cgccgaagcc ttcgccgaga acaccgaagc 77640

ccaccagcgg gccaacctgg ccgtgctcac cgacatggcc gcggtcctcg cccgccgcac 77700

cgcactgccc gtcgtcgcgg tcgcccgcat ggccgggcag tacgccaagc cccgctcccg 77760

gcccttcgac gcgctgggcc ttcccgtcta ccgcggcgac ctggtcaact cgatcgagcc 77820

cacgcccgcc gcgcgcacgc cggacccctc ccggatgctg cgggcccgcg ccgaagcggc 77880

gggcgccatg gccgtggtcc gcgccttccg ccccggcgcc acgcccgacg cgcgggcgcg 77940

gcccggcggc gtgagcgagc tctacgtcag ccacgaggcg ctcgtactcg actacgagcg 78000

gtcctcgctg cgggcggaca ccagcggccc cgaaccccgc ctggccagcg gcctcgggca 78060

cttcctgtgg atcggcgagc gcacccgtga cctggacggc gcgcacatcg cgttcgcgga 78120

gctcctggcc aacccgatcg gcctcaagat cggcccccgt accaccccgg agcaggccgt 78180

cgactacgtg cgcaggctcg acccgcacgg cgaaccgggg cgcctcacgc tgatcagccg 78240

catggggcac acccgggtcc gtgccgtcct gcccgcgatc gtggagaagg tcaccgcctc 78300

gggacaccag gtgatctgga gctgcgaccc catgcacggc aacacgtaca ccgcggcgaa 78360

cggctacaag acccgcgagg tccggcacat cgccgacgag atctccggtt tcttcgccgt 78420

gcaccggcgc ctcggcaccc accccggcgg cctgcacgtc gagctcaccg gcgacgacgt 78480

cacggagtgc gtcggcggcg cccgcggcat caccgaggcc gacctgcccg cccgctaccg 78540

cacggcctgc gacccgcgcc tcaacgcgga gcagtcgatg gaactcgcca ccgccgtcgc 78600

ggacctcgcg gcggccggtc ccacggacag agaacgcacg gaaagctgag gccgcgatga 78660

ccggacaccc tcccggcacg cccatcaagg gcaccaccag gctgtacgcc gtcctcggcg 78720

accccgtcgc ccaggtccag tcgcccggcc tgatgaatcc gctcttcgcg cggctcggca 78780

gggacgccgt gctcgtgccg gtgcacgcca gggcgccgca cctcgacgcc gtcgtgcgcg 78840

gcctgcggcg gatcgacaac ctcgacggca tgttcgtcac cgtgccccac aaggcggccg 78900

tacgccggct cgccgaccgg tgcggcccga ccgtggacgt cgtcggcagc gcgaacgtgc 78960

tgcgccggga ggccgacggc tgctggctcg ccgagaactt cgacggctcg ggcttcgtca 79020

cgggactcgt cgcggccgga cacgaaccca agggcgccca cgtggcgctg gtcgggacgg 79080

gcggcgcggg cagcgccatc gccgcggccc tcctcgccgc gggcgtcgac cgcctgtccg 79140

cgtacgacac ggacaccgcg aagctcggcg ccctgatcac ccgcctcgac gcgcactggc 79200

ccggccgggt ccgcgcgctg gccggaccgc acctgcggga cgtcgacatc gccgtcaacg 79260

cgacgccgct cgggctgcgc cccgacgacc cgctgccctt cgcgccggac ggcctgccgc 79320

cgggcggcgt ggtggccgac atcgtgatga agccgcgcga gacgaagctc ctgcgcgagg 79380

cggccgccct gggccacccc gtccaccacg gcattcacat gctggaaggc caactggagg 79440

cctatcaggc cttcttcggc ctgacctgag gcaggccagg aggcccgccc cgaggcccgc 79500

ccagaggtct gccccgaggt cggcccgagg tccgccccga gcgcgcgccg actacctgac 79560

cgagggatgc ggaggcgtcg tcaggcctct actttggacc tgttggtcat cttttctccc 79620

ctccgcggca cgcggccccg actgtcccgc accccgcgtc gagccgatcc atggttggag 79680

aaacatgaac gtgcgacaga cgccggaatt ccccgagtgg ccgcagtacg acgcgaagga 79740

gcgggaaggc ctcgtccgcg ctctcgaaca gggccagtgg tggcggatgg gcggcagcga 79800

agtcgactcc ttcgagcggg agttcgccga gcaccacggc gccgcccacg ccctggcggt 79860

caccaacggg acacacgcgc tcgaactcgc cctgcagtgc atgggcgtgg gcccgggcac 79920

cgaggtcatc gtcccggcct tcaccttcat ctcctcctcc caggcggccc agcggctcgg 79980

cgcggtcgcg gtcccggtgg acgtcgacga ggacacgtac tccatcgacg tggcggcgac 80040

cgccgccgcc gtcacgccca ggacccgggt gatcatgccc gtgcacatgg cgggcttcat 80100

ggcggacatg gacgccctcg ccaagctctc cgccgacacg ggcgtcccgc tgctccagga 80160

cgccgcgcac gcccacggcg cccgctggcg cggcaggcgc gtcggcgaac tcggctccgt 80220

cgccgcgttc agcttccaga acggcaagct catgaccgcc ggtgagggcg gcgccgtcct 80280

gttccccgac acggagatgt acgagacggc gttcctgcgg cacagctgcg ggcggccccg 80340

caccgaccgc aggtacctgc accggatcgc cggatccaac ctgcggctca acgagttctc 80400

cgcggccgtg ctccgcgccc aactgggccg cctcgaacag caggtgacga cccggcagca 80460

gcgctgggcg ctgctttccg ggctcctcgg cgcgatcgac ggcgtggtcc cgcagcgcgg 80520

cgacgagcgc accgacttcc cctcgcacta catggccatg ttccggatcc ccggcatcgg 80580

cgaggagcag cgcaacgcgc tcgtcgaccg gctcgtggcg gcgggcgtcc cggcgttcgc 80640

gggcttccgc gcgatctacc gcaccgacgc cttctgggag ttcggcgcgc ccgacgagag 80700

ccccgacgag gtcgccgagc gctgcccccg caccgaggcc atcagccagg actgcgtctg 80760

gctccaccac cgggtgctgc tcgccgccga gcaggccctg cacgagaccg cggcgatcgt 80820

cgcggacgcc gtggccgcca tatgagcgtc aggacggccg tcgtcgggct cggctgggcg 80880

ggccgggagc tgtggctgcc gcggctgcgc gcgcacgccg acttcgacgt ggtggccgcc 80940

gtcgacaccg aacccgcggc acgggccgcc ttcgacaccg ccctcggcgt ccccgtgcac 81000

ccgacggccg acgcactcac cgcgcaggac gtcgacctcg ccgtcgtcgc cgtacccaac 81060

catctgcacg ccgaggtggc gggagccctc ctcggccgag gcgtccccgt cttcctggag 81120

aagccggtgt gcctgaccgc cgccgaggcc gacgccctgg ccgccgccga acgcgacggc 81180

gacgccacgc tcctcgcggg cagcgccgcc ccgcaccgcg gcgacgtggc ggccctggcg 81240

gccctggtgc cggacctcgg ccacgtccgg cacgccgatc tttcctgggt cagggcgcgc 81300

ggcgtccccc gggcgggcgg ctggttcacc cggcgcggca cggccggtgg cggagtgctg 81360

ttcgacctcg gctggcacct gttcgacacg ctcgcctcgc tgctcggccc cgcccccttc 81420

acccaggtcg tcggcgtgac gtcgagcgac ttcgtcaacg ccgccgcctg gggcgcgacc 81480

tggcggcggg accggcccgc cgacgacgcc gtcggcgacg tcgaggacac cgcccggggc 81540

ttcctggtcc gcgccgacgg gatctccgtg tcgctgcgcg cggcctgggc gtcgcacgag 81600

gccctggacg tcacccggat ccagctcgtg ggcagcgagg gcaccgccga actgcggtgc 81660

accttcggct tcagccccaa ccgcgccccc gagtcggccc tgaccctgac gcgtgcgggc 81720

accaccacgg gcgtcccggt gccggacgag cccatcggcg acgagtaccg gcgccagctg 81780

gacggcctcg ccaccgtcct gaaggaaccg gccagccgcg gcagggccgt cggcgaggcc 81840

cgcacgacgg tccgcgtcat cgaggacttc tacgcctcgg cccgttccgc acccgcgcgg 81900

aacgtcgggc ccgctcaccg gtggacgggg gaggaccagt gacagcagcg gttccgggag 81960

agccggccga cgcgctggac ggcgatcgcg tgccgggccg ggcgccgggg gcagccgcga 82020

cggacacgct caccccggtc aggcgtgccg tggtcttcga cctggacggg gtgatcgtcg 82080

acagcttcgc ggtgatgaac gaggccttcg ccctcgccta caaggaggtc gtcggcgagg 82140

gcccggcccc cttcgacgag taccggcggc acctggggcg ctacttcccg gacatcatgc 82200

ggatcatggg cctgcccctg gagatggagg agccgttcgt ccgcgagagc taccgcctcg 82260

cccccaaggt gcccgtcttc gagggcgtcg tcgagctgct cctgaccctg cgcgtgcgcg 82320

ggctcaagct cgccgtggcc accggcaaga gcgggccgcg ggcgcgctcc ctgctggagc 82380

agctgggcct cctcccgttc ttcgcccacg tcctcggctc ggacgaggtg ccacggccca 82440

agcccgcgcc cgacatcgtg gagcacgccc tgcgcctgct ggacgtgacc gccgaggagg 82500

ccgtcatggt gggcgacgcg ccgaccgacc tcgccagcgc ccagggcgcg ggagtgaccg 82560

cggtcgccgc gctgtgggcc gtggacgaca cggacgagct gctcgccgcc cggccggacg 82620

ccgtcctgcg ccgcccggcc gacctgctgg acctgtgccc gcccgtgccg ggcgcctgag 82680

ggaggaaggg cgtacggcat ggacagcgcc gggtacctgg gagttgacat cgggggcacc 82740

aaggtcgcct tccgggtgga gaccgaggcg gagtgcgtcc acgagagcgc gttcgcgtgg 82800

gacccgcggc acagcgccgc ccgcgacctc cgccaactcg ccgacgccgt cgccgaggtg 82860

cgggcgcgcc tcggcgcccc cttccgggcg gtgggcgtcg ccatgcccgc gacggccggg 82920

ccggacgagc gcgtcaccgc ctggccgagc agacctgagt ggaccggcac ggacctgggc 82980

gcggccctgc gcccgctgtt ccccgaagca gccgtcgcct gggcggacga cggcgacctc 83040

ggcgcactcg cggagtcccg ggccgcgggc tgcgacgacc tgctctacct cggtgtgggg 83100

acgggcgtcg gcggcggcct cgtcctgcgg ggcgagccgt gccccgggat cggccgcggc 83160

tccttcgagg tcgggcacgt ggtcgtcgag ctcagcggcc cgcggtgcgg ctgcggccgc 83220

aggggctgtc tccaggcggt ggcgtcgggc ccggccaccc tgcgccgcgc gcaccggctg 83280

cgggggagcg cggtcacctt cgacgacctg ctcacggcct ggggcgacgg gcggccgtgg 83340

gccgtcaccg cgctgcgcgg gacctgccgg gctctggccg ccgcggcggt gggcgtccag 83400

gagctgctcc ggccgcgcct cgtacgcgtc ggcggcggct tcgcgagcgg catcccgggg 83460

ttcgccgggc tcgtcgccga ccacgtcacg gaactggccc gccccggact gcccccgctc 83520

ccggtggagc aggccgcgct gggcggcctg tcgtccttgc acggcgcggt gtcactggcc 83580

cggctgatcg cagcgcgccc cgttccggat ttcgtacagc gggaaaagag cgcggaatcc 83640

ttatcaggtt gaggggtgct caaccggtta tggctgccct atcgtcccga agggtcgggc 83700

tgttccggag cgccggcacg cattccggca cggcgcgacg actcatcggc gtaccgacgg 83760

cttatcggca taccgatgat tcatcggtgc gctgaaggcc tatcggtgta ccgacggcat 83820

atcggcgtac cggcgcagca cggagagcac cggcgtggcc cggaggaatt gaggccgccg 83880

gtccttcacc gccccgcacg gcagcgcccg tccctctcgc cgtcccacgt cggtgggtcg 83940

gcaggtcact ttcctcgacg cagaaggggt acccccatga cagccaccgc cgagaacgcg 84000

accgagccga tcgacctgct ctcgccggac ctgactgccg accccttcgc caagtacgcg 84060

cggatgcgcg agcaggcgcc ggtcctcgcg ggcacggtcc tggggtcacc gatgtggctc 84120

gtcaccggct acgacgacgt gcgtctggtc ctcaccgacc cccggttcgt caccgacgcg 84180

acgtccgtac gaggagacga cccggacggc gggcacgcga tcctgaagaa gctcaacatc 84240

ccgtccgacg tggaggacct catcgccaag gcgatgatga acctcgacgg cgccccgcac 84300

gcccggctgc gcaaactggt ctcgcgcgcg ttcaccgccc gacgggtggc cgagatgcgc 84360

ccgcgcatcg aggaactcac cgcgcggctg ctcgacggga tggcggcggc gggccgcgac 84420

ggcacgcccg tcgacctcgt cgaggcgttc tgctacccgg tgccgatcgc cgtgatctgc 84480

gagctggtcg gcatcgacga ggaggaccgg ccgcagtggt cgggctgggg gcgcgtgctg 84540

tccctgatgc aggtcgagca cctccccacc atcctgcgcg agtgctccgc acacgtcatg 84600

gacgtcatcg aacgccgccg cgccgagccg cgcgacgacc tgatctccgc gctcatccag 84660

gcccaggagg acgacggcga ccggctctcc gaccgggaga tggtcacgct cgtcttcacc 84720

atggtcaccg cggggcacga gtcgacgacg tacctcctcg ggaactccgt gctcgcgctc 84780

ctggaacagc cggaccagct cgccctgttg cgtgcggacc cgtcccgatg gcagccggcg 84840

gtgcgcgaac tcatgcgcct gggcccctcg cagttcgcac agccccggta cgcggcgcag 84900

gacgtcgagc tgagcggcgt gcgcatcccc gcgggctccg cggtcacccc gctgctcctg 84960

gccgcgaaca ccgacccgcg caagtacgcg gaccccgagc gcctcgacgt cggcagggga 85020

gccgcgagcc acgtcggctt cggcatgggg gtgcaccact gcctgggcgc gccgctggcc 85080

tatctggagg cggaggtggc gctccaggcg ctgttcgacc ggttccccga cctgtccctc 85140

gcggtgccgc gcgaggagat cccgtgggcg ctgcggcccg gcttcacccg ggtcgcggag 85200

ctgccgctgc gcctggcctg accggccacc gaccgcccgc tcaccgcccg cggcggatcg 85260

cgcgacggcc cgtcagacgg ccccgcgcgg tgcgccggcc gggaccgggg cgcgcagctg 85320

gtcccgcagg tcgtcgagca gctcccggat gccgtcgggc ccgctcgcgg cgccgaagac 85380

gtggtagtcc gggcggacga gcagcgccgt cgccgcgaac cgggcgaggt acggccggta 85440

gacgccatcg gtgtccacca cctcgacgac gccgggcgcc gcggcgtcca ccgcggcggg 85500

cgcgtccttc cgcgcggctc ccggcggcag cagccgcacc acgtgggccg cgaggtcccc 85560

gaggagggcg agccgctcct cgtcgagcac ggcccgcggg tcctcggtgg tcagcagcac 85620

gaaaccgcgg cccacgacgt cgtcgaagag cccgccgaag ccgccccggt ccacgcgccc 85680

ttgcgggacg accgcgcccg cgggcgcggt cgccgcggca ccccggtgca gcagcccgcc 85740

cgggagcggc ttcgcgggct caggccccga cggcacgcgg ccgcgccggt tggcgaggac 85800

ggtggcgtcg cgctcggcgg cggccacggg gtccgtcaca cagatcagcc ggcccagctg 85860

gaccgacgcc aggatcgcct ccttggcctg cgcgcggcgc tcccgcgcat agctgtccag 85920

gagggccggt cctgcctgcc cgcgcagcgt cagatccagc ttccacgcca ggttgacgac 85980

gtcccggatg ccggagcaca tgccctgccc ggcgaacggc ggcatcagat gcgccgcgtc 86040

cccggccagc aggacgtgcc ccacgcgcca ctggtcggcc caactggcct ggaagatgta 86100

cgtggtgctg cgcagcagcg tcgccgtgtc gggagtgacg ccgaacggcg cgagcagccg 86160

ccaggccgcc tcgtccccgc tgagctcgac ggcccgctcg cccggcaggc gcatgaactc 86220

ccagcgccgc cgcccgggac cgctgcccac cagggtcgtg ggccgcgccg ggtcgcagat 86280

ctgcacgttg gtgggggtga acgcgcgcgg ctcgcgcagt tcgacgtcgc agagcagcca 86340

ctcgtaggag aacccgaggt cggtgacggg cacgtcgagg tggtcgcgca cgaagctgtt 86400

ggcgccgtcg cagcccacga cccatcgcgc ggacagggcc cgggtggtgc cgtcgtcggt 86460

ggcggccgtc acctccaccc ggtgctcgcc gtcggcgatc cccacgacct cgtggccgcg 86520

caggaccgtc accccgagcg ccgcggccct ggccgcgatc aactcctcca gggcgggctg 86580

gtgcatcgtg ttggcgtccg gccagccgta ggggcccgcc gtggtgaagg ggatgtcgag 86640

gagcgccgcg cctgcggccg tccgccactg gtagccgttg gccggttcgg tgatccggcc 86700

gaggtccggg ccgatgccgg tggcggccag gagccgggcg gtctccccgt cgaagctggt 86760

ggcgcgcggc agcggatacg gcctgcggcg gcgctccagg acggtcacgc gcagtccgcg 86820

ctgggcgagc agcaccgaaa gcgcggcgcc gaccggtccg ttgcccacga cgacgacatc 86880

cgcgtccgtg gggtccgcgg gcacttccaa aaggggtgtc aaggaaaacc tcacagatgc 86940

gcaccgtttg acgaaatgaa ttccatcgtg ccgaaaagcg ccgacagttg caggaaatcg 87000

ctggcaccgt attccgcagt acgaaacgtg tcaagaagcg gaaaacctta ccaggttgag 87060

ggatgccccg ccggtcattt ccgccctatc ctgccgagga gtcgtgcctc ataaccgtaa 87120

gagcaccggc acttcacgcc gcacggccgc aggaacgtcg gaattcccgt ccgccggtcc 87180

cattcagctc cgcacgcgcg acatccttga cgccgaaggg gagacctatg acagccaccg 87240

ccgagaacgc gaccgagccg gtcgacctgc tctcgccgga cctgatcgcc gacccgttcg 87300

ccgcgtacgc gcggatgcgt gagcaggcgc cgaccctgac cggttcgatc atgggcgggc 87360

gcccgatgtg gctggtcacc cgctacgagg acgtccgcaa ggtgctcacc gacccgcggt 87420

tccacaacaa cccggcatcg gtgccggacg ccccggacac ccggacgacg atcatgcgcc 87480

agctcaacct gccgaccgac ctggtcgact acctcgaaga caagctcatg cagatcgacg 87540

gcgccgacca ccgccggctg cgcggcctgg tctccaaggc gttcacggca cggcgggtgg 87600

cccgcctccg cccccgggtc gaggagatca ccacgacgct gctcgacggg atggcgcagg 87660

cgggccgcga cggcacgccc gtcgacctgt tgaccgcgtt ctgctatccg ctgccgttca 87720

ccgtgatctg cgagctggtc gggatcgacg aggccgaccg cgcgccctgg ttcgagtggg 87780

ggcactgcct gtcctcgccc acggagcacc gcgagcgcct cccgcaggtc atgcgcgagt 87840

gcgtcgacca catgctggag gcgatcgagc ggcgccgcgc cgagccgcgc gacgacctga 87900

tctccgcgct cgtccaggtc caggaggacg acggcgaccg gctctccgag cgggagatgg 87960

tcaccctggt cttcaccctg gtgatcaccg gacacgagac gacgacgcac ctgctcgcca 88020

actcggtgct cgccctcctg gagaacccgg accaactggc cctcgtacgc gccgatccgt 88080

cccggtggcc gcaggcggtg aacgagctca tgcgcctggg gcccacgcag tggggcctgc 88140

cccggcaccc gtcggaggac gtcgaagtcg gcggcgtgac gatccccgcg ggcgccaccg 88200

tcatgccgct gatcctgccc gcgaacaccg atccgcgccg gtacgacgac cccgagcgcc 88260

tcgacatcgg ccgcgagacc gggcagagcc acctcggctt cgggcagggg gcgcactact 88320

gcctcggcgc gccgctggcg ctccaggagg cggaggtggc gctgcacgcg ctgttcgagc 88380

ggttccccgg tctgtccctc gcggtgccgc gcgaggaggt cgtctggacg ctgcgccccg 88440

gcgtcacccg tgtcgagaag cttccgctgc gcctcgtctg agcacacagg acgccgtacc 88500

ccctcgcccg ctgctgccaa gggcgagggg gtacggcgtg tgctgcgggg gatcagagcg 88560

cgtcgacggc gtacggcacg gccagggtcg gctcaggcga ccttcgtcgc gcacaggatg 88620

ccgtgggcga tcagggcccc cggcatggtg gtgaggtcct gggcgaccgt cctggtgacc 88680

cggaacccgg cttcctccag ccacttccgg tacgtcgaca gcttctgcgg cccgccctgc 88740

cccatggtgc aggcgaggaa gtaggcgggg aagatgtcga cgccggcgct gctgggctcc 88800

gtgatgtcct cggggtagac cggcaccagg atgttgacct tcccgccgac gtcgagggcc 88860

tcgctcacgc cgttgaggat cgtgcgcacg tcgtccttgt cgaacatgtc gaggaagtgc 88920

ttgatcagga cgacgtcgaa ccctttgggg acgctcttga agacgtcgcc gccgatgaag 88980

gagcagtgct ccgccacgcc ctcggcccgg aagttctcca ggcactcggc ttccttctcg 89040

ggcaggtcga acgtcgtcac gcgcaggccg ggcgaggcct tgaggcggtg ggtcaggatc 89100

gcgccgagcc cggtgttccc ggccaggtcg agcacccgcg agcccggcgg gatgtggatg 89160

ttgcggaaga accaggggtc catgtgggcg gtgacgtcgt ccatcatcgt cgcccaggac 89220

tcgcgcaggt ccttgtgctc ggccacggcc tcgtacaggt tcccctcgaa tccgtacagc 89280

ttgtccaggc cgacgatgct gccggtcctg acgctgtcgg tgaggtagaa caactgccgg 89340

agcatgacca ccttgatcat gttcaggacg gggagcaccc gctggagatc ggcgtcggac 89400

acgcccgcga acgcgtcgag ggagtagccg ccggaggcct tgtcgaaggc gacgaagccc 89460

tccttgaccg gcaggtgcag gagttgctcc acggcgtccg gcttggcgct gacggcctcg 89520

ccgagctggg ccgcggtcag accgggccgc ttccgcagcg cgtcgacgat gccgagctcg 89580

aaacaggaca gcaggatcat gaaccgcgag ggccccacca tgtactcgcg cagacgcgtg 89640

aacgtggcca ctgcattcat cgcactcatt ccttcgggtc ggctgatcgg ctgccacaac 89700

gtcccgctcc ggcacggcgg cttccgtgcc ccgcactcac tccggtcgcc gggcgacgat 89760

cctggcgtgg tggagaccgg ccttgcccgc ggcgaaggcc gcccgcagca cctcgtccac 89820

gaactcgtcg acgcgctcgc cgagggcgtc ggcctgcgtg gcgaagccca tcagcttgta 89880

ccgcctgcgc atctcggcca gctccgcgtc ggacggcagg gatccgttcc cgcccaggga 89940

gtgcacgatc gccgggtcgt agtcgaccag ttcgagaccg gcgtcccgga gcaccgccgc 90000

cacctcgtcg gccgggtagt cccgcatcgc gggcggcgcc gacgccagca gatcccgcca 90060

cttcgcgctg cccgagacct cgcgcacgat cccgaacatc cccgcgccgt cgccgcgcgg 90120

ctgggcgccg gaccactgga gtacgaggtg gccgcccggg cgcagcgcgc gggccatccg 90180

gcgcagcggc tcctcggcct cggccagcca gtgcagcacc caggaggagt ggatcaggtc 90240

gaactggccc tgtggcagct ccaggcgctc ggccgggcag acatcggccc gcacggagag 90300

ccccgcgcac cgctgccgcg tggcctccac catggacggc gaggcgtcgc tggcccatac 90360

gtcgaagccc cgctccgcga cggcccgcgc gacctctccg gtgccgcacc cgatctcggc 90420

gaaggagccg cccttctcca gggcgccgcc cgcgagttcg agagcggcca gggccgtctt 90480

gatgtgtggc gcgttgacct gcgcgtagtc ctcggccagc caggcgttga ctgcggtcat 90540

cgacgaagtc ctctctctgc cgggaagacc gcggcggccg gagcgcgagc cgtacggcac 90600

gccgctctcg tgcgacaccg cggtcacgag tgcccgggac accggaaggg atgaggcgcc 90660

gtcagagttg agtaccgatg cggtactggg ggccgttgtc ggcccgccgc ggcccctgcc 90720

cttccgtagc ctagggcggc cccgagcggc ttggcacccc tcgatctgat agggcgtcgg 90780

ccggtcagtc ggccagggag agggcctggc ccgggcagtt gtcgacggcc tcgtgggccc 90840

ttcgcaccag gagttcgccc tcgggctcgg cgttcaggac gacgaccgtg ccctcgtcct 90900

cgctctggtc gaacaggccc ggttcggtga acacgcactg gccggagccg acgcatttgt 90960

cggcgtccgc gatgatcttc atggtgatgc ctcctccttg cgaaacccgt cgtgcacggg 91020

ggaaagagcc acccgtcgtg cacgctgaaa gagccgcccg gaaggccgcg tcgggcaccg 91080

ctgaattccg tcggcgccaa cggtgttggc gagtggcgcc gacgggatcg gacgcgcgcc 91140

gcggcacggt gaattgctcc gcggaagtgc cccgatgatc gtgcggaaat gtgcgtggaa 91200

atccgtgagg acctctgtcc gcacgtcgtg cgggatatcg cggggcactt cctggtatcc 91260

gggatgtccg gccgggtaac gttaccaccg gacgtcggac gacggtgccg ggctcagtag 91320

tcggcgctcg aaatccggct ccacgtacgc gtgagttcga cgcgcgaggc cacgttcatc 91380

ttgcggaaga tcttcttgag gtggaaagcc accgtgtgcc cggagatgaa aagccgctca 91440

ccgacctggg agttcgtgaa gccctggctc accagctcgg ccacggccac ctccgaattg 91500

gtgagagagc cgaccttcac gctgtcgtgg gcggcgtact gcggatagcg gccgcgccgc 91560

actcccagtg acctgagcct gctcacgacc cggcgggcat cgcgcggggc cgaggacttc 91620

gagtatccgt ccaccgcccg ttcgaggacg tccaccgccc ggtcgcggtc ctgccgggtg 91680

tccgcgagca gctttccggc gtcttcgtag gcggacgcgc ccgcccacag atcccggtgt 91740

gcctcggccg cggcgatcag gaattccgga ttctcggcca ccagcccgtc ggcgtgcgtt 91800

cccgccgcct ccacggaacg gatcccgcgg ttgcgcgcgg cgagttcgga cgcctcggcc 91860

gccacccgct tggcgaggac ctcgtcgccc agcttccgcg cggcccggac gagccaggcg 91920

gcggcggccg gctgcgcggc aaggagctcc aggatgagct ggtcgtcggt gatgatgccg 91980

acgacgaggt gggcggcgct cggcgcaccg ccctgcacct ccaggagctg ggccgccgcc 92040

caggcgcact ggctggggcg gtggatcgtc tgcccgagga gggcgtcgtc ccggagctga 92100

ttggcgaagt ccgagctcac gctcatgttg ccctgcctga gggcgctgag cgccatcacg 92160

gcgtgcgcct cggggagcag acaggtgagg cccagctgct gtgcctcgcg cacccccttc 92220

tccgccaggt cgacagcggt gtcgatgtcc ccctgggtga aggcgagttc cgccgcgcac 92280

gtcagcgagg ccgcgaccag accgccggac gtcgtggtgc cggccgtcag ctcctccacg 92340

ccggcgagta cgcgtgctcc ggccgccggt tcgcggattc tcaccaggag ggcggagtgc 92400

cagatcctgg cgaggcccac acatttcccc gagcactccg gttgtgtttt cccggctctt 92460

tccgcaaggc gcaggccttc tgtgagatcg ccgcgcgacc aggcggaaaa ggagagtgtg 92520

gcaagccgaa cggtttcgca ggagctgttc tcgcaggccc ccatgacgta cagggaaggc 92580

ggcagctgca ggcggaacgg tgaatcgagc atcacatctc ctgtcggtgg atcatcacta 92640

acgcctggtg gtccgcgcgc ggttccggca gcgccgaccg tgggaagtgt gcacaggaac 92700

ggttgcagtt atggcggaca ggtgcgcaag ggggcgcgcg ccaccactat gtgacgcctc 92760

gtcgactacc gcgtggcgcc ggcttcgccg accgaaggcg ggcagacatg ccgcaggggc 92820

ggcacgagtc ggtgccgacg agggggaact ggctggaggg gggtgggggc tcaagttgca 92880

tctgacatgt tccgatgatc caacgttaag ccccggaagt gcaggagcac ttcagcggag 92940

atgaagcata atttcaaagc tgcggcaggg cctcagaggt gtcgcttgcg atttgaccga 93000

ttttgcagcg atcttcccgg gggcagcttt cctgtggcca attccttact gtggccagtt 93060

tcttacctta gccgcagcgc ggggacttac gggcgagtaa ccctcgcatt cgcgcctgct 93120

tcgtcaactc cggcgcgccg tggggcggtc ggggcgcgcg gggatagggg cttacggggt 93180

ctttgcagga tgatcttggt gtggcacggt gggtgcctgc gcgatcacgt ccgggtgtgc 93240

cgcccgggcg gaagtgggct ggtggggcgc gcagttcggc ccggccggat cgacgggtga 93300

gtcaggtgcg gaccttgcgg cagccatgtg cggagtgacc gtccgggacg gggtggacct 93360

tgtgcgtgaa gtggcccgcg gagcggcccg gggagcggcg tccggcggtg gcgcgaaagg 93420

cctcggtgct caccgggaac ccggtcgggg ccggcggcaa caacgccacc gcccgtatcc 93480

agggtgtcct gcgtgccgac ggcgacaact gccccgcgac gcccgcaagt tcactcaagc 93540

aaaattcaag ccggaactac agctaccgtt tcggagcaat agcagccggg taggtcttca 93600

ggtcggatca atagggggcg ggtagttcgg atcgaggcaa gaggagccgg gtaggtcatc 93660

agtatttctg gagggaagcg ttttgggaaa gccatccaca cgcgttctgc tggtaagcaa 93720

tgatgcgcgt ctgagcactg ccctgcacca cccaccgctc cccgcgaagt acttcgtcat 93780

ctcggtgccg accagcacgc aggccctgga gaacttcggc gatttcgacc tcttgctgct 93840

cgactcctcc acacccggta tggacagcca cgaggtgtgc aaaagaatcc gcaaccaggc 93900

gtcggtgccc atcatcattc tcggcccctg cgtgaatgag atcgaccggg tactggcgct 93960

gaacgccggg gcggactact gcgtggtgaa atcggactcg ctccacgagt tgcaggcgag 94020

catggacgcc gtcatgcggc gctcgtcccc ctgtccgacc ggtcagaatg ctccggggtc 94080

agccgctcag ggcggcgaga tcctggtcgc cgggccgctg cggctgaatc cgcgcaaccg 94140

ggacgtgacc ttggcgggac ggccggtcag gctgaccccc aaggagttcg atctgctcgc 94200

catgctgatg gaggatccgg acaccctgca cacccgcaag gccatcatcg cgcgggtgtg 94260

gaacgagcac tggttcggct cgactcggac cctggacgtc catatcgggg cgctgcgtca 94320

gaaactcggt tcctacgaat ggatcgagac caaacgcggg atcggattcc ggctcggcac 94380

ggtgccggtg cccggtgccc gggactcgcc gtagccgtgg gcatcacggc cgcgtctgcc 94440

cgccgacctt gccgttcgag ccgcgcgggc tccgcggggc cgccgcggtg aggtgcaaca 94500

gggcgcgggc ggccaggcga tagccgaagg ccccaagacc gaccacgact ccgctcgcca 94560

acggagcgat caccgactcg tggcggaatt gctcgcgggc ccacacgtta ctgagatgca 94620

cttcgaccca cggcggcgca tagctcgcca gcgcgtcgcg cagactccat cccgcaatca 94680

tgagggcgcc cggattgacg atggcgccga ccgtgctctc gccgtgccgg tgaatcgcgc 94740

cgacgagatc cccttccgaa tcgtgctgga cggagaggac ttcccatccc cgggcccgca 94800

cttcatcggc gaccgctgcc tcgatgtcgg cgagcgtatc ggtgccgtat atctcgggct 94860

cacgtcggcc cagaacgccc aggttgggtc cgttgaggag cagcacgaca ctcaagggga 94920

acctcgctat tccttgggaa ggcggacggt cggcggaccg gggaccgctc cccataatcg 94980

gcgatcccgg gcaccgcaca ccatcatcag aattgatagt tccggtggcg cgcgggagcg 95040

gccgacgtgc tgcgcggcag ggcggacaac aggtctacca gcatgggtag acctgggtgt 95100

cgaggcgatt gcggacgccc gcgcgtatac cactccgcgt atccgccgcg tacgctggcg 95160

gcaactcctt gacggagcgg gggagacggg ggaacggggg ttgatggctt tcggccgagc 95220

gatccggtgc gacggtccgc accagtgcgg ctgcacggca caggacggca cggaacagaa 95280

cggcacggcg cggtgcggca ccgc 95304

<210>2

<211>357

<212>PRT

<213>Streptomyces spectabilis

<400>2

Met Ala Thr Val Pro Leu Arg Leu Asp Asp His Ala Tyr Asp Val Leu

1 5 10 15

Ile Gly Pro Gly Val Arg Ala Ser Leu Thr Asp Val Val Arg Arg Leu

20 25 30

Gly Ala Gly Arg Ala Val Val Val Ser Ala Arg Pro Ala Glu Trp Val

35 40 45

Pro Asp Thr Gly Val Glu Thr Leu Leu Leu Arg Ala Arg Asp Gly Glu

50 55 60

Arg Asp Lys Thr Leu Ala Thr Val Glu Ala Leu Cys Gly Glu Phe Val

65 70 75 80

Arg Phe Gly Leu Thr Arg Ser Asp Val Val Val Ser Cys Gly Gly Gly

85 90 95

Thr Thr Thr Asp Val Val Gly Leu Ala Ala Ala Leu Tyr His Arg Gly

100 105 110

Val Pro Val Val His Leu Pro Thr Ser Leu Leu Ala Gln Val Asp Ala

115 120 125

Ser Val Gly Gly Lys Thr Ala Val Asn Leu Ser Ala Gly Lys Asn Leu

130 135 140

Val Gly Ala Tyr Trp Gln Pro Ser Ala Val Leu Cys Asp Thr Asp Tyr

145 150 155 160

Leu Thr Thr Leu Pro Arg Arg Glu Leu Leu Asn Gly Leu Gly Glu Ile

165 170 175

Ala Arg Cys His Phe Ile Gly Ala Ala Asp Leu Arg Gly Leu Pro Leu

180 185 190

Val Glu Gln Ile Thr Ala Ser Val Thr Leu Lys Ala Arg Ile Val Glu

195 200 205

Ala Asp Glu Arg Asp Thr Gly Val Arg His Ile Leu Asn TyrGly His

210 215 220

Thr Leu Gly His Ala Leu Glu Lys Ala Thr Asp Tyr Ala Leu Arg His

225 230 235 240

Gly Glu Ala Val Ala Val Gly Thr Val Phe Ala Gly Arg Leu Ala Gly

245 250 255

Ala Leu Gly Arg Ile Gly Pro Ala Arg Val Ala Glu His Gln Asp Val

260 265 270

Val Ala Tyr Tyr Gly Leu Pro Ala Ala Leu Pro Ala Glu Ala Glu Pro

275 280 285

Ala Asp Leu Leu Arg Leu Met Arg His Asp Lys Lys Ala Ile Thr Gly

290 295 300

Leu Ala Phe Ala Leu Asp Gly Pro His Gly Ala Glu Leu Val Ser Asp

305 310 315 320

Val Pro Pro Glu Val Val Ala Arg Val Leu Glu Arg Met Pro Arg Ala

325 330 335

Pro Leu Ala Asp Leu Val Gly Ala Ala Ala Thr Gly Ala Ala Ala Asn

340 345 350

Asp Ala Ala Arg Thr

355

<210>3

<211>384

<212>PRT

<213>Streptomyces spectabilis

<400>3

Val Ala Ser Leu Ser Arg Ala Ala Pro Ile Val Thr Pro Ala Glu Thr

1 5 10 15

Ala Arg Leu Ala Asp Arg Leu Ala Ala Val Ala Arg Gly Glu Ala Phe

20 25 30

Leu Leu Gln Gly Gly Asp Cys Ala Glu Ala Phe Ala Glu Asn Thr Glu

35 40 45

Ala His Gln Arg Ala Asn Leu Ala Val Leu Thr Asp Met Ala Ala Val

50 55 60

Leu Ala Arg Arg Thr Ala Leu Pro Val Val Ala Val Ala Arg Met Ala

65 70 75 80

Gly Gln Tyr Ala Lys Pro Arg Ser Arg Pro Phe Asp Ala Leu Gly Leu

85 90 95

Pro Val Tyr Arg Gly Asp Leu Val Asn Ser Ile Glu Pro Thr Pro Ala

100 105 110

Ala Arg Thr Pro Asp Pro Ser Arg Met Leu Arg Ala Arg Ala Glu Ala

115 120 125

Ala Gly Ala Met Ala Val Val Arg Ala Phe Arg Pro Gly Ala Thr Pro

130 135 140

Asp Ala Arg Ala Arg Pro Gly Gly Val Ser Glu Leu Tyr Val Ser His

145 150 155 160

Glu Ala Leu Val Leu Asp Tyr Glu Arg Ser Ser Leu Arg Ala Asp Thr

165 170 175

Ser Gly Pro Glu Pro Arg Leu Ala Ser Gly Leu Gly His Phe Leu Trp

180 185 190

Ile Gly Glu Arg Thr Arg Asp Leu Asp Gly Ala His Ile Ala Phe Ala

195 200 205

Glu Leu Leu Ala Asn Pro Ile Gly Leu Lys Ile Gly Pro Arg Thr Thr

210 215 220

Pro Glu Gln Ala Val Asp Tyr Val Arg Arg Leu Asp Pro His Gly Glu

225 230 235 240

Pro Gly Arg Leu Thr Leu Ile Ser Arg Met Gly His Thr Arg Val Arg

245 250 255

Ala Val Leu Pro Ala Ile Val Glu Lys Val Thr Ala Ser Gly His Gln

260 265 270

Val Ile Trp Ser Cys Asp Pro Met His Gly Asn Thr Tyr Thr Ala Ala

275 280 285

Asn Gly Tyr Lys Thr Arg Glu Val Arg His Ile Ala Asp Glu Ile Ser

290 295 300

Gly Phe Phe Ala Val His Arg Arg Leu Gly Thr His Pro Gly Gly Leu

305 310 315 320

His Val Glu Leu Thr Gly Asp Asp Val Thr Glu Cys Val Gly Gly Ala

325 330 335

Arg Gly Ile Thr Glu Ala Asp Leu Pro Ala Arg Tyr Arg Thr Ala Cys

340 345 350

Asp Pro Arg Leu Asn Ala Glu Gln Ser Met Glu Leu Ala Thr Ala Val

355 360 365

Ala Asp Leu Ala Ala Ala Gly Pro Thr Asp Arg Glu Arg Thr Glu Ser

370 375 380

<210>4

<211>270

<212>PRT

<213>Streptomyces spectabilis

<400>4

Met Thr Gly His Pro Pro Gly Thr Pro Ile Lys Gly Thr Thr Arg Leu

1 5 10 15

Tyr Ala Val Leu Gly Asp Pro Val Ala Gln Val Gln Ser Pro Gly Leu

20 25 30

Met Asn Pro Leu Phe Ala Arg Leu Gly Arg Asp Ala Val Leu Val Pro

35 40 45

Val His Ala Arg Ala Pro His Leu Asp Ala Val Val Arg Gly Leu Arg

50 55 60

Arg Ile Asp Asn Leu Asp Gly Met Phe Val Thr Val Pro His Lys Ala

65 70 75 80

Ala Val Arg Arg Leu Ala Asp Arg Cys Gly Pro Thr Val Asp Val Val

85 90 95

Gly Ser Ala Asn Val Leu Arg Arg Glu Ala Asp Gly Cys Trp Leu Ala

100 105 110

Glu Asn Phe Asp Gly Ser Gly Phe Val Thr Gly Leu Val Ala Ala Gly

115 120 125

His Glu Pro Lys Gly Ala His Val Ala Leu Val Gly Thr Gly Gly Ala

130 135 140

Gly Ser Ala Ile Ala Ala Ala Leu Leu Ala Ala Gly Val Asp Arg Leu

145 150 155 160

Ser Ala Tyr Asp Thr Asp Thr Ala Lys Leu Gly Ala Leu Ile Thr Arg

165 170 175

Leu Asp Ala His Trp Pro Gly Arg Val Arg Ala Leu Ala Gly Pro His

180 185 190

Leu Arg Asp Val Asp Ile Ala Val Asn Ala Thr Pro Leu Gly Leu Arg

195 200 205

Pro Asp Asp Pro Leu Pro Phe Ala Pro Asp Gly Leu Pro Pro Gly Gly

210 215 220

Val Val Ala Asp Ile Val Met Lys Pro Arg Glu Thr Lys Leu Leu Arg

225 230 235 240

Glu Ala Ala Ala Leu Gly His Pro Val His His Gly Ile His Met Leu

245 250 255

Glu Gly Gln Leu Glu Ala Tyr Gln Ala Phe Phe Gly Leu Thr

260 265 270

<210>5

<211>386

<212>PRT

<213>Streptomyces spectabilis

<400>5

Met Asn Val Arg Gln Thr Pro Glu Phe Pro Glu Trp Pro Gln Tyr Asp

1 5 10 15

Ala Lys Glu Arg Glu Gly Leu Val Arg Ala Leu Glu Gln Gly Gln Trp

20 25 30

Trp Arg Met Gly Gly Ser Glu Val Asp Ser Phe Glu Arg Glu Phe Ala

35 40 45

Glu His His Gly Ala Ala His Ala Leu Ala Val Thr Asn Gly Thr His

50 55 60

Ala Leu Glu Leu Ala Leu Gln Cys Met Gly Val Gly Pro Gly Thr Glu

65 70 75 80

Val Ile Val Pro Ala Phe Thr Phe Ile Ser Ser Ser Gln Ala Ala Gln

85 90 95

Arg Leu Gly Ala Val Ala Val Pro Val Asp Val Asp Glu Asp Thr Tyr

100 105 110

Ser Ile Asp Val Ala Ala Thr Ala Ala Ala Val Thr Pro Arg Thr Arg

115 120 125

Val Ile Met Pro Val His Met Ala Gly Phe Met Ala Asp Met Asp Ala

130 135 140

Leu Ala Lys Leu Ser Ala Asp Thr Gly Val Pro Leu Leu Gln Asp Ala

145 150 155 160

Ala His Ala His Gly Ala Arg Trp Arg Gly Arg Arg Val Gly Glu Leu

165 170 175

Gly Ser Val Ala Ala Phe Ser Phe Gln Asn Gly Lys Leu Met Thr Ala

180 185 190

Gly Glu Gly Gly Ala Val Leu Phe Pro Asp Thr Glu Met Tyr Glu Thr

195 200 205

Ala Phe Leu Arg His Ser Cys Gly Arg Pro Arg Thr Asp Arg Arg Tyr

210 215 220

Leu His Arg Ile Ala Gly Ser Asn Leu Arg Leu Asn Glu Phe Ser Ala

225 230 235 240

Ala Val Leu Arg Ala Gln Leu Gly Arg Leu Glu Gln Gln Val Thr Thr

245 250 255

Arg Gln Gln Arg Trp Ala Leu Leu Ser Gly Leu Leu Gly Ala Ile Asp

260 265 270

Gly Val Val Pro Gln Arg Gly Asp Glu Arg Thr Asp Phe Pro Ser His

275 280 285

Tyr Met Ala Met Phe Arg Ile Pro Gly Ile Gly Glu Glu Gln Arg Asn

290 295 300

Ala Leu Val Asp Arg Leu Val Ala Ala Gly Val Pro Ala Phe Ala Gly

305 310 315 320

Phe Arg Ala Ile Tyr Arg Thr Asp Ala Phe Trp Glu Phe Gly Ala Pro

325 330 335

Asp Glu Ser Pro Asp Glu Val Ala Glu Arg Cys Pro Arg Thr Glu Ala

340 345 350

Ile Ser Gln Asp Cys Val Trp Leu His His Arg Val Leu Leu Ala Ala

355 360 365

Glu Gln Ala Leu His Glu Thr Ala Ala Ile Val Ala Asp Ala Val Ala

370 375 380

Ala Ile

385

<210>6

<211>271

<212>PRT

<213>Streptomyces spectabilis

<400>6

Val Cys Leu Thr Ala Ala Glu Ala Asp Ala Leu Ala Ala Ala Glu Arg

1 5 10 15

Asp Gly Asp Ala Thr Leu Leu Ala Gly Ser Ala Ala Pro His Arg Gly

20 25 30

Asp Val Ala Ala Leu Ala Ala Leu Val Pro Asp Leu Gly His Val Arg

35 40 45

His Ala Asp Leu Ser Trp Val Arg Ala Arg Gly Val Pro Arg Ala Gly

50 55 60

Gly Trp Phe Thr Arg Arg Gly Thr Ala Gly Gly Gly Val Leu Phe Asp

65 70 75 80

Leu Gly Trp His Leu Phe Asp Thr Leu Ala Ser Leu Leu Gly Pro Ala

85 90 95

Pro Phe Thr Gln Val Val Gly Val Thr Ser Ser Asp Phe Val Asn Ala

100 105 110

Ala Ala Trp Gly Ala Thr Trp Arg Arg Asp Arg Pro Ala Asp Asp Ala

115 120 125

Val Gly Asp Val Glu Asp Thr Ala Arg Gly Phe Leu Val Arg Ala Asp

130 135 140

Gly Ile Ser Val Ser Leu Arg Ala Ala Trp Ala Ser His Glu Ala Leu

145 150 155160

Asp Val Thr Arg Ile Gln Leu Val Gly Ser Glu Gly Thr Ala Glu Leu

165 170 175

Arg Cys Thr Phe Gly Phe Ser Pro Asn Arg Ala Pro Glu Ser Ala Leu

180 185 190

Thr Leu Thr Arg Ala Gly Thr Thr Thr Gly Val Pro Val Pro Asp Glu

195 200 205

Pro Ile Gly Asp Glu Tyr Arg Arg Gln Leu Asp Gly Leu Ala Thr Val

210 215 220

Leu Lys Glu Pro Ala Ser Arg Gly Arg Ala Val Gly Glu Ala Arg Thr

225 230 235 240

Thr Val Arg Val Ile Glu Asp Phe Tyr Ala Ser Ala Arg Ser Ala Pro

245 250 255

Ala Arg Asn Val Gly Pro Ala His Arg Trp Thr Gly Glu Asp Gln

260 265 270

<210>7

<211>194

<212>PRT

<213>Streptomyces spectabilis

<400>7

Met Asn Glu Ala Phe Ala Leu Ala Tyr Lys Glu Val Val Gly Glu Gly

1 5 10 15

Pro Ala Pro Phe Asp Glu Tyr Arg Arg His Leu Gly Arg Tyr Phe Pro

20 25 30

Asp Ile Met Arg Ile Met Gly Leu Pro Leu Glu Met Glu Glu Pro Phe

35 40 45

Val Arg Glu Ser Tyr Arg Leu Ala Pro Lys Val Pro Val Phe Glu Gly

50 55 60

Val Val Glu Leu Leu Leu Thr Leu Arg Val Arg Gly Leu Lys Leu Ala

65 70 75 80

Val Ala Thr Gly Lys Ser Gly Pro Arg Ala Arg Ser Leu Leu Glu Gln

85 90 95

Leu Gly Leu Leu Pro Phe Phe Ala His Val Leu Gly Ser Asp Glu Val

100 105 110

Pro Arg Pro Lys Pro Ala Pro Asp Ile Val Glu His Ala Leu Arg Leu

115 120 125

Leu Asp Val Thr Ala Glu Glu Ala Val Met Val Gly Asp Ala Pro Thr

130 135 140

Asp Leu Ala Ser Ala Gln Gly Ala Gly Val Thr Ala Val Ala Ala Leu

145 150 155 160

Trp Ala Val Asp Asp Thr Asp Glu Leu Leu Ala Ala Arg Pro Asp Ala

165 170 175

Val Leu Arg Arg Pro Ala Asp Leu Leu Asp Leu Cys Pro Pro Val Pro

180 185 190

Gly Ala

<210>8

<211>317

<212>PRT

<213>Streptomyces spectabilis

<400>8

Met Asp Ser Ala Gly Tyr Leu Gly Val Asp Ile Gly Gly Thr Lys Val

1 5 10 15

Ala Phe Arg Val Glu Thr Glu Ala Glu Cys Val His Glu Ser Ala Phe

20 25 30

Ala Trp Asp Pro Arg His Ser Ala Ala Arg Asp Leu Arg Gln Leu Ala

35 40 45

Asp Ala Val Ala Glu Val Arg Ala Arg Leu Gly Ala Pro Phe Arg Ala

50 55 60

Val Gly Val Ala Met Pro Ala Thr Ala Gly Pro Asp Glu Arg Val Thr

65 70 75 80

Ala Trp Pro Ser Arg Pro Glu Trp Thr Gly Thr Asp Leu Gly Ala Ala

85 90 95

Leu Arg Pro Leu Phe Pro Glu Ala Ala Val Ala Trp Ala Asp Asp Gly

100 105 110

Asp Leu Gly Ala Leu Ala Glu Ser Arg Ala Ala Gly Cys Asp Asp Leu

115120 125

Leu Tyr Leu Gly Val Gly Thr Gly Val Gly Gly Gly Leu Val Leu Arg

130 135 140

Gly Glu Pro Cys Pro Gly Ile Gly Arg Gly Ser Phe Glu Val Gly His

145 150 155 160

Val Val Val Glu Leu Ser Gly Pro Arg Cys Gly Cys Gly Arg Arg Gly

165 170 175

Cys Leu Gln Ala Val Ala Ser Gly Pro Ala Thr Leu Arg Arg Ala His

180 185 190

Arg Leu Arg Gly Ser Ala Val Thr Phe Asp Asp Leu Leu Thr Ala Trp

195 200 205

Gly Asp Gly Arg Pro Trp Ala Val Thr Ala Leu Arg Gly Thr Cys Arg

210 215 220

Ala Leu Ala Ala Ala Ala Val Gly Val Gln Glu Leu Leu Arg Pro Arg

225 230 235 240

Leu Val Arg Val Gly Gly Gly Phe Ala Ser Gly Ile Pro Gly Phe Ala

245 250 255

Gly Leu Val Ala Asp His Val Thr Glu Leu Ala Arg Pro Gly Leu Pro

260 265 270

Pro Leu Pro Val Glu Gln Ala Ala Leu Gly Gly Leu Ser Ser Leu His

275280 285

Gly Ala Val Ser Leu Ala Arg Leu Ile Ala Ala Arg Pro Val Pro Asp

290 295 300

Phe Val Gln Arg Glu Lys Ser Ala Glu Ser Leu Ser Gly

305 310 315

<210>9

<211>183

<212>PRT

<213>Streptomyces spectabilis

<400>9

Met Gly Ser Gly Pro Arg Ser Ala Asp Arg Pro Pro Ser Gln Gly Ile

1 5 10 15

Ala Arg Phe Pro Leu Ser Val Val Leu Leu Leu Asn Gly Pro Asn Leu

20 25 30

Gly Val Leu Gly Arg Arg Glu Pro Glu Ile Tyr Gly Thr Asp Thr Leu

35 40 45

Ala Asp Ile Glu Ala Ala Val Ala Asp Glu Val Arg Ala Arg Gly Trp

50 55 60

Glu Val Leu Ser Val Gln His Asp Ser Glu Gly Asp Leu Val Gly Ala

65 70 75 80

Ile His Arg His Gly Glu Ser Thr Val Gly Ala Ile Val Asn Pro Gly

85 90 95

Ala Leu Met Ile Ala Gly Trp Ser Leu Arg Asp Ala Leu Ala Ser Tyr

100 105 110

Ala Pro Pro Trp Val Glu Val His Leu Ser Asn Val Trp Ala Arg Glu

115 120 125

Gln Phe Arg His Glu Ser Val Ile Ala Pro Leu Ala Ser Gly Val Val

130 135 140

Val Gly Leu Gly Ala Phe Gly Tyr Arg Leu Ala Ala Arg Ala Leu Leu

145 150 155 160

His Leu Thr Ala Ala Ala Pro Arg Ser Pro Arg Gly Ser Asn Gly Lys

165 170 175

Val Gly Gly Gln Thr Arg Pro

180

<210>10

<211>5557

<212>PRT

<213>Streptomyces spectabilis

<400>10

Val Pro Gly Ala His Ala Tyr Asp Thr Leu Ala Val Thr Glu Pro Pro

1 5 10 15

Val Pro Ala Arg Asp Asp Leu Gly Leu Asp Asp Val Ala Trp Met Phe

20 25 30

Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly Val Leu Ser Thr Gln

35 40 45

Arg Asn Cys Leu Trp Ser Val Ala Ser Cys Tyr Val Pro IlePro Gly

50 55 60

Leu Thr Asp Arg Asp Arg Val Leu Trp Pro Leu Pro Leu Phe His Ser

65 70 75 80

Leu Ser His Ile Ala Cys Val Leu Ser Val Thr Val Val Gly Ala Thr

85 90 95

Ala Arg Ile Met Asp Gly Ser Ser Ala Asp Asp Val Ile Glu Ala Leu

100 105 110

Arg Glu Gly Gly Ala Thr Phe Leu Ala Gly Val Pro Thr Thr Tyr His

115 120 125

His Leu Val Thr Ala Ala Arg Gln Asn Gly Phe Ser Ala Pro Ala Leu

130 135 140

Arg Ile Gly Leu Val Gly Gly Ala Val Thr Gly Pro Gly Leu Arg Arg

145 150 155 160

Asp Phe Glu Asp Thr Phe Gly Val Pro Leu Val Asp Ala Tyr Gly Ser

165 170 175

Thr Glu Thr Cys Gly Ala Ile Thr Ile Asn Pro Pro Asp Gly Ala Arg

180 185 190

Val Asp Gly Ser Cys Gly Leu Pro Val Pro Gly Val Gly Val Arg Val

195 200 205

Val Asp Pro Gly Thr Gly Leu Asp Val Pro Ala Gly Gln Glu Gly Glu

210 215 220

Val Trp Val Ser Gly Pro Asn Val Met Val Gly Tyr His Asn Ser Pro

225 230 235 240

Glu Ala Thr Ala Glu Ala Leu Arg Asp Gly Trp Tyr Arg Thr Gly Asp

245 250 255

Leu Ala Arg Arg Asp Asp Ala Gly Tyr Phe Thr Ile Arg Gly Arg Ile

260 265 270

Lys Glu Leu Val Ile Arg Gly Gly Glu Asn Ile His Pro Glu Glu Ile

275 280 285

Glu Ala Val Leu Arg Thr Val Glu Gly Val Ala Asp Ala Ala Val Ala

290 295 300

Gly Ala Pro His Ala Thr Leu Gly Glu Val Pro Val Ala Tyr Val Val

305 310 315 320

Pro Gly Pro Ala Gly Phe Asp Thr Ala Thr Leu Leu Asp Arg Cys Arg

325 330 335

Glu Arg Leu Ser Ala Tyr Lys Val Pro Glu Arg Ile His Glu Val Thr

340 345 350

Gly Ile Pro Arg Thr Ala Ser Gly Lys Thr Arg Arg Arg Leu Leu Ala

355 360 365

Asp Arg Pro Ser Arg Leu Arg Tyr Ala Ala Ser Gly His His Glu Gly

370 375 380

Leu Leu Ser Leu Asp Trp Ala Ser Val Ala Ala Pro Ala Asp Pro Gly

385 390 395 400

Pro Pro Ala Arg Asp Trp Ala Val Val Gly Pro Ala Ala Ala Gly Leu

405 410 415

Gly Thr Ala Leu Lys Ala Ala Gly Ala Asp Val Arg His Tyr Pro Asp

420 425 430

Leu Ala Ala Val Arg Ala Ala Ala Ala Asp Glu Asp Ala Ala Pro Glu

435 440 445

Val Thr Val Val Val Arg Pro Pro Val Thr Gly Val Val Arg Pro Ser

450 455 460

Ala Pro Ala Ala Pro Gly Gly Ala Arg Glu Ala Ile Ala Ala Gly Arg

465 470 475 480

Glu Gly Ala Arg Leu Trp Ala Asp Glu Leu Arg Ser Trp Leu Glu Arg

485 490 495

Pro Glu Ser Ala Ala Ser Arg Leu Val Val Leu Thr Arg Ala Ala Val

500 505 510

Val Ala Ser Ala Gln Asp Asp Arg Gln Asp Leu Ala Ala Ala Pro Leu

515 520 525

Trp Ala Val Val Pro Ser Phe Gln Glu Gly Gly Ala Gly Ser Leu Thr

530 535 540

Leu Leu Asp Leu Asp Ala Asp Ala Asp Pro Asp Ile Leu Cys Ala Ala

545 550 555 560

Leu Ala Thr Gly Glu Pro Arg Ile Ala Val Arg Ala Gly Ala Leu Leu

565 570 575

Ala Pro Arg Pro Ser Arg Leu Pro Val Ala Asp Gly Pro Gly Ala Asp

580 585 590

Gly Arg Gly Thr Asp Gly Pro Gly Ala Glu Ala Trp Pro Gly Ala Thr

595 600 605

Gly Thr Val Val Val Thr Gly Ala Asp Thr Glu His Gly Ala Ala Leu

610 615 620

Ala His Arg Leu Val Thr Val His Gln Val Glu Ser Leu Leu Leu Val

625 630 635 640

Asp Thr Pro Gly Ser Ala Gly Thr Pro Thr Ala Pro Ala Pro Pro Gly

645 650 655

Ala Asp Ile Val Arg Val Ala Ala Asp Gly Gln His Pro Glu Gln Leu

660 665 670

Arg Ala Ala Leu Ala Asp Leu Pro Arg Pro Val Ser Ala Val Val His

675 680 685

Ala Ala Asp Asp Pro Glu Leu Ala Leu Leu Leu Asp Glu Met Thr Ala

690 695 700

Ala Asn Gly Leu Ala Ala Phe Val Ala Val Ser His Thr Ala Ala Pro

705 710 715 720

Leu Gly Thr Pro Asp Arg Leu Gly Ala Ala Val Asp Gly Ala Leu Thr

725 730 735

Glu Ala Val Val Arg Asp Arg Arg Leu Arg Asn Leu Pro Gly Thr Phe

740 745 750

Leu Ala Arg Ala Asp Gly Val Asp Ile Arg Ala Gly Gly Ala Asp Thr

755 760 765

Ala Trp Ala Gly Ser Pro Leu Leu Ser Ala Phe Asp Leu Ala Leu Thr

770 775 780

Thr Asn Pro Pro Thr Leu Leu Ala Leu Asp Pro Ser Ala Leu Ala Asp

785 790 795 800

Ser Ser Arg Leu Pro Thr Leu Leu Ala Thr Leu Thr Glu Pro Gln Pro

805 810 815

Arg Arg Ser Glu Pro Asp Asp Ala Val Ala Ala Ala Leu Arg Glu Arg

820 825 830

Leu Ala Ala Leu Asp Glu Arg Ala Gln Arg Ala Leu Leu Glu Asp Leu

835 840 845

Val Arg Thr Arg Ala Ala Ala Val Leu His Arg Ser Gly Ser Glu Pro

850 855 860

Ile Pro Ser Asp Arg Ala Phe Arg Asp Leu Gly Phe Thr Ser Met Ala

865 870 875 880

Val Val Glu Leu Arg Asn Arg Leu Thr Glu Ser Thr Gly Leu Pro Leu

885 890 895

Pro Thr Thr Val Val Phe Asp His Pro Thr Pro Glu Ala Leu Ala Ala

900 905 910

Arg Leu Arg Ala Glu Ile Leu Gly Val Pro Gln Pro Val Thr Glu Pro

915 920 925

Val Pro Ala Ala Asp Pro Gly Glu Pro Leu Ala Ile Val Gly Met Ala

930 935 940

Cys Arg Leu Pro Gly Gly Val Ala Gly Pro Glu Asp Leu Trp Gln Leu

945 950 955 960

Val Ala Glu Gly Arg Asp Ala Val Ser Gly Phe Pro Glu Asp Arg Gly

965 970 975

Trp Asp Leu Glu Gly Leu Phe Asp Pro Asp Pro Asp Arg Ala Gly Thr

980 985 990

Ser Tyr Thr Asp Gln Gly Gly Phe Leu Arg Gly Ala Gly Leu Phe Asp

995 1000 1005

Ala Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala Met Asp

1010 1015 1020

Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Ala Leu Glu

1025 1030 1035

Arg Ala Gly Val Asp Pro Leu Ser Leu Lys Gly Ser Asp Val Gly

1040 1045 1050

Val Phe Ser Gly Val Phe Gly Gln Gly Tyr Gly Ala Thr Gly Thr

1055 1060 1065

Gly Val Val Ala Pro Glu Val Glu Gly Phe Ala Ser Thr Gly Ser

1070 1075 1080

Ala Gly Ser Val Ala Ser Gly Arg Val Ser Tyr Val Phe Gly Phe

1085 1090 1095

Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu

1100 1105 1110

Val Ala Ile His Leu Ala Ala Gln Ala Leu Arg Gln Gly Glu Cys

1115 1120 1125

Ser Met Ala Leu Ala Gly Gly Ala Thr Val Met Ala Thr Pro Gly

1130 1135 1140

Thr Phe Val Glu Phe Ser Arg Gln Arg Ala Leu Ala Pro Asp Gly

1145 1150 1155

Arg Cys Lys Ala Phe Ser Ser Ser Ala Asp Gly Thr Gly Trp Ala

1160 1165 1170

Glu Gly Ala Gly Val Val ValLeu Glu Arg Leu Ser Val Ala Arg

1175 1180 1185

Glu Arg Gly His His Val Leu Ala Val Leu Arg Gly Ser Ala Val

1190 1195 1200

Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro

1205 1210 1215

Ser Gln Gln Arg Val Ile Arg Arg Ala Leu Ala Gly Ala Gly Leu

1220 1225 1230

Ala Pro Ala Asp Val Asp Ala Val Glu Ala His Gly Thr Gly Thr

1235 1240 1245

Ala Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr

1250 1255 1260

Gly Gln Asp Arg Asp Pro Gln Gln Pro Leu Trp Leu Gly Ser Leu

1265 1270 1275

Lys Ser Asn Leu Gly His Thr Gln Ala Ala Ala Gly Val Ser Gly

1280 1285 1290

Val Ile Lys Met Val Gln Ala Leu Arg His Gly Val Leu Pro Ala

1295 1300 1305

Thr Leu His Val Ala Ala Pro Thr Pro Gln Val Asp Trp Ser Ala

1310 1315 1320

Gly Ala Val Glu Leu Leu Thr Glu Ala Arg Glu Trp Pro Arg Asn

1325 13301335

Gly Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Val Ser Gly

1340 1345 1350

Thr Asn Ala His Val Ile Leu Glu Glu Ala Pro Glu Glu Pro Val

1355 1360 1365

Gln Ala Gly Ala Ala Ser Ala Ser Ala Val Pro Leu Val Val Ser

1370 1375 1380

Ala Arg Ser Pro Arg Ser Leu Ala Gly Gln Ala Arg Arg Leu Val

1385 1390 1395

Ser Tyr Val Glu Ser Gly Ala Asp Gly Val Ser Leu Ala Gly Ala

1400 1405 1410

Ala Arg Ala Leu Val Ser Gly Arg Ala Val Leu Gly Glu Arg Ala

1415 1420 1425

Val Val Val Ala Asp Ser Gly Glu Glu Ala Leu Ala Gly Leu Arg

1430 1435 1440

Ala Leu Ala Arg Gly Glu Ser Gly Pro Asn Leu Val Thr Gly Ser

1445 1450 1455

Gly Gly Val Pro Gly Lys Val Val Trp Val Phe Pro Gly Gln Gly

1460 1465 1470

Ser Gln Trp Val Gly Met Gly Arg Glu Leu Leu Asp Ser Ser Ser

1475 1480 1485

Val Phe Val Glu Arg Val Ala Glu Cys Ala Ala Ala Leu Glu Arg

1490 1495 1500

Trp Val Asp Trp Ser Leu Val Asp Val Leu Arg Gly Glu Val Glu

1505 1510 1515

Pro Ala Leu Leu Glu Arg Val Asp Val Val Gln Pro Ala Gly Phe

1520 1525 1530

Ala Val Met Val Gly Leu Ala Ser Val Trp Ala Ser Val Gly Val

1535 1540 1545

Lys Pro Asp Ala Val Leu Gly His Ser Gln Gly Glu Ile Ala Ala

1550 1555 1560

Ala Cys Val Ala Gly Ala Leu Ser Leu Glu Asp Ala Ala Arg Val

1565 1570 1575

Val Ala Leu Arg Ser Gln Ala Ile Ala Ala Arg Leu Ala Gly Arg

1580 1585 1590

Gly Gly Met Ala Ser Val Ala Leu Ser Glu Glu Asp Ala Ala Gly

1595 1600 1605

Arg Leu Glu Pro Trp Ala Asp Arg Val Glu Val Ala Ala Val Asn

1610 1615 1620

Gly Pro Ser Ser Val Val Val Ala Gly Asp Ala Glu Ala Leu Asp

1625 1630 1635

Glu Ala Leu Arg Glu Leu Ser Gly Asp Gly Val Arg Val Arg Arg

1640 1645 1650

Val Ala Val Asp Tyr Ala Ser His Thr Arg His Val Glu Asp Ile

1655 1660 1665

Glu Asp Thr Leu Ala Glu Ala Leu Ala Gly Val Thr Ala Lys Ala

1670 1675 1680

Pro Val Val Pro Phe Tyr Ser Thr Val Thr Gly Ser Trp Val Glu

1685 1690 1695

Asp Ala Gly Val Leu Asp Gly Arg Tyr Trp Tyr Arg Asn Leu Arg

1700 1705 1710

Gly Gln Val Arg Phe Gly Pro Ala Val Arg Glu Leu Leu Gly Gln

1715 1720 1725

Gly His Gly Val Phe Leu Glu Val Ser Ala His Pro Val Leu Val

1730 1735 1740

Gln Pro Ile Thr Glu Ala Val Asp Ala Ala Asp Ala Asp Val Val

1745 1750 1755

Val Thr Gly Ser Leu Arg Arg Asp Glu Gly Gly Leu Arg Arg Leu

1760 1765 1770

Leu Ala Ser Met Ala Glu Leu Phe Val Arg Gly Leu Ala Val Asp

1775 1780 1785

Trp Ala Ala Val Leu Pro Ala Gly Ala Ala Arg Ala Glu Leu Pro

1790 1795 1800

Thr Tyr Ala Phe Asp His Glu His Tyr Trp Leu Glu Pro Ala Asp

18051810 1815

Arg Ala Thr Asp Ala Ala Ser Leu Gly Gln Thr Ala Gly Asp His

1820 1825 1830

Pro Leu Leu Gly Ala Val Val Gly Met Pro Asp Ser Ser Gly Leu

1835 1840 1845

Met Ala Thr Ser Arg Trp Ser Leu Arg Ser Gln Pro Trp Leu Ala

1850 1855 1860

Asp His Leu Ala Ser Asp Ala Val Val Val Pro Asn Ala Ala Leu

1865 1870 1875

Val Glu Val Ala Val Arg Leu Gly Asp Leu Ala Ala Thr Pro Val

1880 1885 1890

Leu Asp Glu Leu Ala Val Asp Ala Pro Val Val Leu Pro Arg Arg

1895 1900 1905

Gly Ser Arg Gly Ile Gln Ala Val Val Gly Glu Pro Asp Gly Ala

1910 1915 1920

Gly Arg Arg Ser Val Glu Ile Tyr Ser Arg Ala Gly Asp Ala Pro

1925 1930 1935

Leu Asp Ala Arg Trp Thr Arg His Ala His Gly Thr Leu Ser Thr

1940 1945 1950

Arg Ala Val Glu Val Pro Ala Ala Gly Pro Ala Thr Glu Val Ala

1955 1960 1965

Leu Asp Gly Ala Ala Leu Arg Asp Ala Gly Arg Tyr Gly Val His

1970 1975 1980

Pro Ala Leu Leu Glu Ala Ala Val Arg Thr Val Ile Ala Asp Gly

1985 1990 1995

Thr Val Pro Ala Val Trp Thr Gly Val Thr Leu Leu Ala Ser Gly

2000 2005 2010

Ala Thr Ala Leu His Val Arg Ala Gly Asp Thr Thr Pro Glu Gly

2015 2020 2025

Thr Gly Leu Arg Leu Thr Asp Pro Thr Gly Gln Pro Val Met Thr

2030 2035 2040

Val Ala Ser Val Arg Gly Ala Ala Phe Cys Pro Glu Gln Ala Glu

2045 2050 2055

Gln Ala Gly Ala Asp Gly Ala Val Thr His Asp Ala Leu Phe Arg

2060 2065 2070

Val Asp Trp Arg Glu Leu Ala Leu Pro Ala Val Glu Arg Pro Pro

2075 2080 2085

Ala Ser Val Thr Val Ala Thr Gly Glu Asp Val Thr Thr Thr Ala

2090 2095 2100

Ala Gly Pro Val Ala Pro Asp Val Leu Leu Tyr Glu Ala Val Arg

2105 2110 2115

Ala Ala Ala Asp Pro Arg Val Pro Val Asn Ala Ala Leu Ala Val

2120 2125 2130

Leu Gln Ala Trp Leu Ala Glu Pro Ala Leu Ala Arg Thr Arg Leu

2135 2140 2145

Ala Val Val Thr Gly Asp His Ala Glu Leu Asp Ala Ala Ala Val

2150 2155 2160

Trp Gly Leu Val Arg Ser Ala Gln Ser Glu His Pro Gly Arg Ile

2165 2170 2175

Val Leu Val Asp Leu Asp Glu Ala Ser Arg Asp Ala Leu Pro Ala

2180 2185 2190

Ala Leu Leu Ser Gly Glu Pro Gln Leu Arg Val Arg Asp Gly Val

2195 2200 2205

Ala Ser Val Pro Arg Leu Ala Arg Ala Ala Val Pro Ala Gly Ala

2210 2215 2220

Ala Pro Ala Gly Ala Gly Arg Arg Pro Arg Pro Leu Asp Pro Glu

2225 2230 2235

Gly Thr Val Leu Ile Thr Gly Gly Thr Gly Thr Leu Gly Ala Val

2240 2245 2250

Thr Ala Arg His Leu Val Ala Ala His Gly Val Arg His Leu Val

2255 2260 2265

Leu Ala Gly Arg Arg Gly Gln Ala Pro Glu Leu Arg Asp Glu Leu

2270 2275 2280

Thr Gly Met Gly Ala Ser Val Thr Leu Ala Ala Cys Asp Thr Ala

2285 2290 2295

Asp Arg Ala Gln Val Ala Ala Leu Leu Arg Ala Ile Pro Ala Ala

2300 2305 2310

His Pro Leu Thr Ala Val Val His Ala Ala Gly Val Leu Asp Asp

2315 2320 2325

Gly Val Leu Thr Glu Leu Thr Pro Glu Arg Leu Asp Thr Val Leu

2330 2335 2340

Arg Pro Lys Leu Asp Ala Ala Arg His Leu His Glu Leu Thr Arg

2345 2350 2355

Asp Leu Asp Leu Ala Ala Phe Val Leu Phe Ser Ser Ala Ala Gly

2360 2365 2370

Val Leu Gly Asn Pro Gly Gln Ala Asn Tyr Ala Ala Ala Asn Ala

2375 2380 2385

Ala Leu Asp Ala Leu Ala Arg Gln Arg His Arg Ala Gly Leu Pro

2390 2395 2400

Ala Val Ser Leu Ala Trp Gly Tyr Trp Ala Thr Ala Ser Gly Met

2405 2410 2415

Thr Ala His Leu Gly Ala Ala Asp Leu Arg Arg Asn Arg Arg Ile

2420 2425 2430

Gly Met Val Gly Leu Ser Pro Ala Glu Gly Met Ala Leu Leu Asp

2435 2440 2445

Ala Ala Leu Asn Thr Pro Ala Asp Ala Ala Gly Thr Leu Val Ala

2450 2455 2460

Ala Lys Phe Asp Leu Asn Ala Leu Arg Ala Ala Ala Ala Glu Glu

2465 2470 2475

Pro Leu Pro Pro Leu Leu Arg Gly Leu Ala Pro Ala Pro Arg Pro

2480 2485 2490

Thr Ala Arg Thr Ala Ala Pro Ala Gly Pro Ala Ala Leu Arg Glu

2495 2500 2505

Arg Val Thr Gly Leu Ala Ala Ala Glu Arg Thr Glu Ala Leu Val

2510 2515 2520

Asp Leu Val Arg Arg His Ala Ala Glu Val Leu Gly His Thr Thr

2525 2530 2535

Ala Asp Ala Val Arg Ala Asp Arg Thr Phe Lys Glu Ala Gly Phe

2540 2545 2550

Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Ala Ala Ala

2555 2560 2565

Thr Gly Leu Thr Leu Ser Pro Ala Met Val Phe Asp His Pro Lys

2570 2575 2580

Pro Thr Ala Leu Ala Ala His Leu Gly Asp Arg Leu Leu Gly Val

2585 2590 2595

Ala Ala Gln Arg Pro Ala Gly Pro Gly Ala Pro Ala Pro Thr Thr

26002605 2610

Asp Glu Pro Ile Ala Ile Val Ala Met Ala Cys Arg Phe Pro Ala

2615 2620 2625

Gly Val His Ser Pro Glu Asp Leu Trp Arg Val Val Ala Asp Gly

2630 2635 2640

Ile Asp Ala Val Thr Glu Phe Pro Ala Asp Arg Gly Trp Asp Thr

2645 2650 2655

Asp Arg Leu Phe His Ala Asp Pro Asp His Glu Gly Thr Thr Tyr

2660 2665 2670

Val Arg His Gly Ala Phe Leu Asp Asp Ala Ala Gly Phe Asp Ala

2675 2680 2685

Ala Phe Phe Gly Ile Ser Pro Asn Glu Ala Leu Ala Met Asp Pro

2690 2695 2700

Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Ala Phe Glu Arg

2705 2710 2715

Ala Ala Ile Asp Pro Thr Ala Leu Ala Gly Gln Asp Val Gly Val

2720 2725 2730

Phe Val Gly Val Asn Ser His Asp Tyr Ser Val Arg Met His Arg

2735 2740 2745

Ala Ala Gly Val Glu Gly Phe Arg Leu Thr Gly Gly Ser Gly Ser

2750 2755 2760

Val Ala Ser Gly Arg Val Ala Tyr His Phe Gly PheGlu Gly Pro

2765 2770 2775

Ala Leu Thr Ile Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Leu

2780 2785 2790

His Thr Ala Ala Gln Ala Leu Asn Arg Gly Glu Cys Ser Met Ala

2795 2800 2805

Leu Ala Gly Gly Val Met Val Met Gly Thr Val Glu Thr Phe Val

2810 2815 2820

Glu Phe Ser Arg Gln Arg Gly Leu Ala Pro Asp Gly Arg Cys Lys

2825 2830 2835

Ala Phe Ala Asp Gly Ala Asp Gly Thr Gly Trp Ser Glu Gly Val

2840 2845 2850

Gly Leu Leu Leu Val Glu Arg Leu Ser Asp Ala Arg Arg Arg Gly

2855 2860 2865

His Pro Val Leu Ala Val Val Arg Gly Ser Ala Val Asn Gln Asp

2870 2875 2880

Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ala Gln Gln

2885 2890 2895

Arg Val Ile Arg Lys Ala Leu Ala His Ala Gly Leu Gly Thr Gln

2900 2905 2910

Asp Val Asp Ala Val Glu Ala His Gly Thr Gly Thr Thr Leu Gly

2915 2920 2925

Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly Gln Asp

2930 2935 2940

Arg Pro Ala Gly Arg Pro Leu Trp Leu Gly Ser Val Lys Ser Asn

2945 2950 2955

Leu Gly His Thr Gln Ala Ala Ala Gly Val Ala Gly Val Ile Lys

2960 2965 2970

Met Val Met Ala Met Arg His Asp Thr Leu Pro Lys Thr Leu His

2975 2980 2985

Val Asp Arg Pro Ser Ser Arg Ile Asp Trp Ser Ala Gly Ala Val

2990 2995 3000

Glu Leu Leu Thr Gln Ala Arg Asp Trp Pro Arg Glu Gly Arg Pro

3005 3010 3015

Arg Arg Ala Ala Val Ser Ser Phe Gly Ile Gly Gly Thr Asn Ala

3020 3025 3030

His Val Val Ile Glu Glu Ala Pro Gly Thr Ala Ala Ala Glu Pro

3035 3040 3045

Asp Pro Arg Arg Ala Glu Glu Pro Thr Asp Leu Pro Val Pro Val

3050 3055 3060

Pro Val Ser Ala Arg Thr Pro Ala Gly Leu Arg Gly Gln Ala Asp

3065 3070 3075

Arg Leu Ala Arg Phe Leu Asp Asp Arg Thr Asp Leu Arg Leu Thr

3080 3085 3090

Asp Ala Ala His Ala Leu Ala Thr Thr Arg Ala Arg Leu Asp His

3095 3100 3105

Arg Ala Val Val Leu Ala Ser Asp Arg Pro Gln Leu Gly Ala Asp

3110 3115 3120

Leu Arg Ala Val Gly Arg Gly Glu Glu Arg Pro Ala Val Val Ser

3125 3130 3135

Gly Thr Pro Val Ala Gly Lys Leu Ala Val Leu Phe Thr Gly Gln

3140 3145 3150

Gly Ser Gln Trp Ala Gly Met Gly Arg Gln Leu Ala Ala Thr Tyr

3155 3160 3165

Pro Val Phe Arg Asp Ala Phe Ala Ala Ala Cys Ala Ala Val Glu

3170 3175 3180

Ala His Leu Ser Gly Leu Ala Ala Arg Pro Leu His Asp Val Val

3185 3190 3195

Phe Ala Ala Pro Gly Ala Pro Gly Ser Glu Leu Leu Asp Arg Thr

3200 3205 3210

Met Tyr Thr Gln Ala Ala Leu Phe Ala Leu Glu Thr Ala Leu Phe

3215 3220 3225

Arg Leu Phe Glu Ser Trp Gly Val Arg Pro Asp Leu Leu Ala Gly

3230 3235 3240

His Ser Ile GlyGlu Leu Thr Ala Ala His Val Ser Gly Val Leu

3245 3250 3255

Gly Leu Ala Asp Ala Gly Glu Leu Val Ala Ala Arg Gly Arg Leu

3260 3265 3270

Met Gln Ala Leu Pro Glu Gly Gly Ala Met Val Ala Val Gln Ala

3275 3280 3285

Thr Glu Ala Asp Val Glu Pro Leu Leu Ala Arg Ala His Gly Ala

3290 3295 3300

Val Cys Val Ala Ala Val Asn Gly Pro Asp Ala Leu Val Leu Ser

3305 3310 3315

Gly Ala Glu Asn Ala Val Leu Ala Ala Ala Asp Ala Leu Ala Arg

3320 3325 3330

Arg Gly Arg Lys Thr Arg Arg Leu Ala Val Ser His Ala Phe His

3335 3340 3345

Ser Pro Leu Met Asp Pro Met Leu Asp Glu Phe Arg Ala Val Ala

3350 3355 3360

Glu Arg Leu Ser Tyr Arg Pro Gly Asp Leu Pro Val Val Ser Thr

3365 3370 3375

Leu Thr Gly Asp Leu Ala Gln Asp Gly Gln Leu Ala Asp Pro Gly

3380 3385 3390

His Trp Ala Asp Gln Val Arg Asn Thr Val Arg Phe Gly Asp Ala

3395 34003405

Val Thr Ala Leu Ala Arg Arg Gly Ala Thr Thr Phe Leu Glu Leu

3410 3415 3420

Gly Pro Gly Gly Thr Leu Ala Ala Met Ala Leu Gly Ser Pro Asp

3425 3430 3435

Ala Pro Glu Arg Ser Cys Val Ala Thr Leu Arg Lys Asp Gly Ala

3440 3445 3450

Glu Ala Val Asp Val Leu Thr Ala Leu Ala Glu Leu His Val Arg

3455 3460 3465

Gly Thr Ser Val Asp Trp Thr Ala Val Leu Gly His Pro Ala Ala

3470 3475 3480

Ala Ala Gly Thr Glu Leu Pro Thr Tyr Ala Phe Gln His Gln Arg

3485 3490 3495

Tyr Trp Val Glu Ser Asp Asp Ala Ala Ser Ala Asp Ala Glu Ala

3500 3505 3510

Leu Gly Thr Thr Gly Ala Arg His Pro Leu Leu Gly Thr Val Ile

3515 3520 3525

Asp Val Pro Gly Asp Asp Gly Gly Asn Gly Ser Gly Gly Val Val

3530 3535 3540

Leu Thr Gly Arg Leu Ala Pro His Gly Pro Gly Arg Leu Pro Gly

3545 3550 3555

Pro Ala Ala Ala Asp Gly Thr Thr Arg Ile Pro Ala Ala Val Leu

3560 3565 3570

Leu Asp Met Ile Val Gly Ala Gly Asp Glu Val Gly Cys Gly Gly

3575 3580 3585

Leu Glu Gln Val Val Val Glu Ser Pro Leu Thr Val Pro Glu Arg

3590 3595 3600

Gly His Thr His Val Arg Val Ser Val Thr Ala Arg Gly Pro Asp

3605 3610 3615

Gly Arg Arg Arg Ala Ala Val His Gly Arg Pro Ala Gly Pro Glu

3620 3625 3630

Arg Thr Pro Trp Thr Cys His Ala Arg Ala Val Leu Val Pro Gly

3635 3640 3645

Ala Pro Arg Pro Ala Phe Asp Leu Arg Ala Trp Gln Pro Pro Ala

3650 3655 3660

Asp Ala Ser Ala Pro Leu Pro Gly Thr Arg Val Trp Gln Gln Asp

3665 3670 3675

Gly Arg Thr Cys Ala Glu Ile Thr Leu Pro Asp Glu Leu Val Ala

3680 3685 3690

Glu Ala Ala Asp Phe Ala Leu His Pro Val Leu Val Asp Thr Ala

3695 3700 3705

Leu Arg Val Leu Asp Arg Ser Pro Gly Arg Asp Leu Ser Asp Cys

3710 3715 3720

Ala Ala Leu Ala Val Tyr Ala Glu Gly Ala Thr Ser Leu Arg Leu

3725 3730 3735

Arg Ile Thr Pro Arg Pro Asp Gly Arg His Leu Leu Glu Leu Ala

3740 3745 3750

Asp Ala Asp Gly Glu Pro Val Ala Ala Leu Gly Pro Leu Ser Leu

3755 3760 3765

Glu Ala Leu Pro Gly Glu Gly Pro Ala Ser Arg Asp Ser Ala Pro

3770 3775 3780

Tyr Glu Asp Ala Gly Val Pro Ala Val Pro Gly Val Thr Arg Arg

3785 3790 3795

Val Val Ala Arg Gly Glu Asp Thr Gly Gly Ala Met Ala Asp Arg

3800 3805 3810

Leu Thr Asp Arg Leu Thr Gly Arg Arg Thr Asp Gln Leu Ala Asp

3815 3820 3825

Arg Leu Ala Asp Arg Leu Ala Gly Leu Pro Ala Ala Glu Gln His

3830 3835 3840

Arg Arg Val Leu Ser Leu Val Gln Glu Ser Thr Ala Val Val Leu

3845 3850 3855

Gly His Arg Gln Gly Asp Arg Phe Asp Asp Glu Gln Pro Phe Lys

3860 3865 3870

Asn Leu Gly Phe Asp Ser Leu Ser Ala Val Lys Leu Arg Asn Arg

3875 3880 3885

Leu Arg Asp Phe Thr Gly Val Asp Leu Pro Ser Ser Leu Val Phe

3890 3895 3900

Asp His Pro Thr Pro Ala Leu Leu Ala Ala Phe Leu Arg Ala Glu

3905 3910 3915

Leu Leu Gly Glu Gln Pro Ala Ala Pro Ala Pro Ala Thr Ala Ala

3920 3925 3930

Ala Ala Ser Asp Glu Pro Ile Ala Ile Val Ala Met Ser Ser Arg

3935 3940 3945

Leu Pro Gly Gly Ala Asp Ser Pro Glu Glu Leu Trp Gln Leu Leu

3950 3955 3960

Val Glu Gly Arg Asp Ala Val Ser Gly Phe Pro Val Asp Arg Asp

3965 3970 3975

Trp Asp Leu Glu Gly Leu Tyr His Pro Asp Pro Gly His Pro Gly

3980 3985 3990

Thr Ser Tyr Thr Arg Ser Gly Gly Phe Leu His Glu Ala Ala Arg

3995 4000 4005

Phe Asp Ala Gly Leu Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala

4010 4015 4020

Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Ala

4025 4030 4035

Leu Glu Arg Ala Gly Val AspPro Leu Ser Ala Arg Gly Ser Asp

4040 4045 4050

Ile Gly Val Phe Thr Gly Ile Val His His Asp Tyr Val Ser Arg

4055 4060 4065

Leu His Arg Val Pro Glu Asp Val Gln Gly Tyr Leu Met Thr Gly

4070 4075 4080

Thr Ala Ser Ser Val Ala Ser Gly Arg Val Ser Tyr Val Phe Gly

4085 4090 4095

Phe Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser

4100 4105 4110

Leu Val Ala Met His Leu Ala Ala Gln Ala Leu Arg Gln Gly Glu

4115 4120 4125

Cys Ser Met Ala Leu Ala Gly Gly Ala Thr Val Met Ala Gly Pro

4130 4135 4140

Asp Ala Phe Leu Glu Phe Ser Arg Gln Arg Gly Leu Ser Ala Asp

4145 4150 4155

Gly Arg Cys Lys Ala Phe Ser Ala Thr Ala Asp Gly Thr Gly Trp

4160 4165 4170

Ala Glu Gly Val Gly Val Val Val Leu Glu Arg Leu Ser Val Ala

4175 4180 4185

Arg Glu Arg Gly His Gln Val Leu Ala Val Ile Arg Gly Ser Ala

4190 41954200

Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly

4205 4210 4215

Pro Ser Gln Gln Arg Val Ile Arg Arg Ala Leu Asp Thr Ala Gly

4220 4225 4230

Leu Ala Pro Ala Asp Val Asp Val Val Glu Ala His Gly Thr Gly

4235 4240 4245

Thr Ala Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr

4250 4255 4260

Tyr Gly Gln Gly Arg Gly Ala Glu Gln Pro Leu Trp Leu Gly Ser

4265 4270 4275

Leu Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala Gly Val Ala

4280 4285 4290

Gly Val Ile Lys Met Val Leu Ala Leu Arg His Ala Val Met Pro

4295 4300 4305

Arg Thr Leu His Val Asp Glu Pro Thr Ala Glu Val Asp Trp Ser

4310 4315 4320

Ala Gly Ala Val Glu Leu Leu Thr Glu Ala Arg Asn Trp Glu Arg

4325 4330 4335

Asn Gly Arg Pro Arg Arg Ala Gly Val Ser Ala Phe Gly Ala Ser

4340 4345 4350

Gly Thr Asn Ala His Leu Ile Leu Glu Glu Ala Pro Glu Asp Pro

4355 4360 4365

Val Ala Ala Glu Thr Val Pro Val Gly Val Val Pro Leu Val Val

4370 4375 4380

Ser Ala His Ser Gln Ala Ser Leu Ala Gly Gln Ala Gly Arg Leu

4385 4390 4395

Val Ser Tyr Val Glu Ser Gly Thr Gly Gly Pro Ser Leu Ala Gly

4400 4405 4410

Val Ala Gly Ala Leu Val Ser Gly Arg Ala Val Leu Gly Glu Arg

4415 4420 4425

Ala Val Val Val Ala Asp Ser Pro Thr Glu Ala Leu Thr Gly Leu

4430 4435 4440

Arg Ala Leu Ala Arg Gly Glu Ser Gly Pro His Leu Val Ser Gly

4445 4450 4455

Ser Ala Ala Pro Gly Ala Pro Gly Lys Val Val Leu Val Phe Pro

4460 4465 4470

Gly Gln Gly Ser Gln Trp Ala Gly Met Gly Arg Glu Leu Leu Asp

4475 4480 4485

Ala Ser Pro Val Phe Ala Glu Arg Ile Ala Glu Cys Ala Ala Ala

4490 4495 4500

Leu Glu Pro Trp Leu Asp Trp Ser Leu Ile Asp Val Leu Arg Gly

4505 4510 4515

Glu Ala Glu Pro Glu Leu Leu Glu Arg Val Asp Val Val Gln Pro

4520 4525 4530

Ala Ser Phe Ala Val Met Ala Gly Leu Ala Ser Val Trp Ala Ser

4535 4540 4545

Val Gly Val Glu Pro Asp Ala Val Leu Gly His Ser Gln Gly Glu

4550 4555 4560

Ile Ala Ala Ala Cys Val Ala Gly Ala Leu Ser Leu Glu Asp Ala

4565 4570 4575

Ala Arg Val Val Ala Leu Arg Ser Arg Thr Ile Ala Ala Glu Leu

4580 4585 4590

Ala Gly Arg Gly Gly Met Ala Ser Val Ala Leu Gly Glu Gln Glu

4595 4600 4605

Ala Ala Ala Arg Leu Glu Arg Trp Ala Asp Arg Val Glu Val Ala

4610 4615 4620

Ala Val Asn Ser Pro Ser Ser Val Val Ile Ala Gly Asp Ala Ala

4625 4630 4635

Ala Leu Asp Glu Val Leu Glu Glu Leu Ser Gly Ala Gly Val Arg

4640 4645 4650

Val Arg Arg Val Ala Val Asp Tyr Ala Ser His Ser Arg His Val

4655 4660 4665

Glu Asp Ile Glu Asn Thr Leu Ala Glu Ala Leu Ala Gly Val Asn

46704675 4680

Ala Lys Ala Pro Val Val Pro Phe Tyr Ser Thr Val Thr Gly Ser

4685 4690 4695

Trp Val Glu Asp Thr Gly Val Leu Asp Gly Ser Tyr Trp Tyr Arg

4700 4705 4710

Asn Leu Arg Gly Gln Val Arg Phe Gly Pro Ala Val Ala Asp Leu

4715 4720 4725

Leu Ser Gln Gly His Gly Val Phe Val Glu Ala Ser Ala His Pro

4730 4735 4740

Val Leu Val Gln Pro Val Thr Glu Ala Val Asp Gly Thr Asp Ala

4745 4750 4755

Glu Ala Val Val Thr Gly Ser Leu Arg Arg Glu Asp Gly Gly Leu

4760 4765 4770

Arg Arg Leu Leu Thr Ser Ala Ala Glu Leu Phe Val Arg Gly Val

4775 4780 4785

Ala Val Asp Trp Ala Gly Val Leu Pro Ala Gly Ala Leu Ala Arg

4790 4795 4800

Pro Ala Arg Ala Asp Leu Pro Thr Tyr Ala Phe Asp Arg Arg His

4805 4810 4815

Tyr Trp Leu Arg Glu Ala Glu Thr Thr Ala Asp Ala Asp Gly Ala

4820 4825 4830

Ala Asp Ser Ser Asp Ser Ala Phe Trp Ser Ala Val Glu Gln Ala

4835 4840 4845

Asp Thr Asp Ser Leu Gly Ala Leu Leu Glu Thr Ala Ser Gly Asp

4850 4855 4860

Gln Leu Gly Ala Leu Ala Thr Leu Val Pro Val Leu Ala Glu Trp

4865 4870 4875

Arg Gly Lys Arg Arg Arg Arg Ser Ala Ala Glu Lys Leu Arg Tyr

4880 4885 4890

His Val Thr Trp Gln Pro Leu Ala Arg Glu Ala Thr Gly Val Pro

4895 4900 4905

Gly Gly Arg Trp Leu Val Val Val Pro Ala Glu Arg Thr Asp Asp

4910 4915 4920

Ser Gly Thr Asp Ala Leu Leu Asp Glu Leu Thr Gly Leu Gly Leu

4925 4930 4935

Asp Ala Leu Leu Leu Glu Val Gly Ala Ser Asp Gly Gly Arg Glu

4940 4945 4950

Arg Leu Ala Glu Arg Leu Ala Gly Val Leu Ala Glu Asp Asp Val

4955 4960 4965

Thr Gly Val Leu Ser Leu Leu Ala Leu Asp Arg Ser Ala Arg Gly

4970 4975 4980

Ala Pro Gln Asp Pro Thr Ala Val Thr Ala Ala Ala Leu Ala Leu

4985 4990 4995

Ile Gln Ala Leu Gly Asp Thr Gly Ala Ala Pro Pro Leu Trp Cys

5000 5005 5010

Val Thr Arg Gly Ala Val Asn Ile Gly Ile His Asp Ala Leu Thr

5015 5020 5025

Ser Pro Ala Gln Ala Ala Leu Trp Gly Leu Gly Arg Ala Ala Ala

5030 5035 5040

Leu Glu Arg Leu Asp Arg Trp Gly Gly Leu Val Asp Leu Pro Ala

5045 5050 5055

Ala Thr Gly Pro Arg Thr Ala Arg Gln Leu Leu Gly Val Leu Asn

5060 5065 5070

Ser Ala Ala Gly Glu Asp Gln Leu Ala Val Arg Arg Ser Gly Val

5075 5080 5085

Tyr Ala Arg Arg Leu Val Arg Lys Ala Val Pro Glu Ala Ala Gly

5090 5095 5100

Asp Thr Gly Trp Gln Pro Arg Gly Thr Val Leu Val Thr Gly Gly

5105 5110 5115

Ala Glu Gly Leu Gly Arg His Val Ser Leu Trp Leu Ala Leu Ala

5120 5125 5130

Gly Val Asp Arg Leu Leu Val Thr Thr Thr Pro His Ala Pro Glu

5135 5140 5145

Gly Ser Thr Ala Glu Leu Arg Ser Gln Ile Ala Gly Leu Gly Leu

5150 5155 5160

Ala Thr Val Val Glu Ser Cys Ala Asp Thr Asp Arg Asp Ala Leu

5165 5170 5175

Ala Arg Leu Val Ser Ala Thr Pro Pro Glu Gln Pro Leu Thr Ala

5180 5185 5190

Val Val His Ala Ala Asp Ile Thr Arg Thr Ser Thr Val Asp Asp

5195 5200 5205

Thr Gly Ala His Asp Leu Ala Glu Val Phe Ala Ala Lys Val Asp

5210 5215 5220

Thr Ala Val Trp Leu Asp Glu Leu Phe Glu Asp Thr Pro Leu Asp

5225 5230 5235

Ala Phe Val Val Phe Ser Ser Ile Ala Gly Val Trp Gly Gly Gly

5240 5245 5250

Gly Gln Gly Pro Ser Gly Ala Ala Asn Ala Val Leu Asp Ala Leu

5255 5260 5265

Val Glu Trp Arg Arg Ala Arg Gly Leu Arg Ala Thr Ser Ile Ala

5270 5275 5280

Trp Gly Ala Leu Asp Gly Ile Gly Val Gly Met Asp Glu Ala Ala

5285 5290 5295

Leu Ala Gln Leu Arg Arg Arg Gly Val Leu Pro Met Ala Pro Gln

5300 5305 5310

Leu Ala Val Thr Ala Met Val Gln Ala Val Gln Gly Asp Glu Lys

5315 5320 5325

Phe Val Thr Val Ala Asp Met Asp Trp Gly Ala Phe Val Pro Ala

5330 5335 5340

Phe Thr Ser Val Arg Ala Ser Pro Leu Phe Ala Asp Leu Pro Glu

5345 5350 5355

Ala Lys Ala Val Leu Asp Ala Ala Arg Asp Asp Gly Glu Asp Ser

5360 5365 5370

Asp Thr Ala Ala Ser Leu Ala Asp Ser Leu Arg Ala Val Pro Asp

5375 5380 5385

Ala Glu Gln His Arg Leu Leu Leu Arg Leu Val Arg Gly His Ala

5390 5395 5400

Ser Thr Val Leu Gly His Gly Gly Ala Glu Gly Ile Gly Pro Arg

5405 5410 5415

Gln Ala Phe Gln Glu Val Gly Phe Asp Ser Leu Ala Ala Val Asn

5420 5425 5430

Leu Arg Asn Ser Leu His Gly Ala Thr Gly Leu Arg Leu Pro Ala

5435 5440 5445

Thr Leu Ile Phe Asp Tyr Pro Thr Pro Glu Ala Leu Val Gly His

5450 5455 5460

Leu Arg Thr Glu Leu Leu Lys Glu Ala Asp Asp Gly Leu Ala Gly

54655470 5475

Arg Glu Asp Asp Leu Arg Arg Val Leu Ala Ser Val Pro Leu Thr

5480 5485 5490

Arg Phe Lys Glu Ala Gly Val Leu Asp Ala Leu Leu Gly Leu Ala

5495 5500 5505

Glu Thr Glu Thr Gly Thr Gly Ala Glu Ala Glu Thr Asp Thr Gly

5510 5515 5520

Ala Ala Ala Gly Ala Ala Thr Arg Thr Gly Ala Ala Asp Ala Glu

5525 5530 5535

Leu Ile Asp Ala Met Asp Val Ala Asp Leu Val Gln Arg Ala Leu

5540 5545 5550

Gly Lys Thr Ser

5555

<210>11

<211>3587

<212>PRT

<213>Streptomyces spectabilis

<400>11

Met Ser Ala Pro Asp Glu Gln Val Ile Glu Ala Leu Arg Ala Ser Leu

1 5 10 15

Lys Glu Asn Ala Arg Leu Gln Gln Glu Asn Ser Thr Leu Val Ala Ala

20 25 30

Ala Ala Glu Pro Val Ala Ile Val Ser Met Ala Cys Arg Tyr Ala Gly

35 40 45

Gly Val Arg Gly Pro Glu Asp Leu Trp Arg Val Val Ser Glu Gly Ala

50 55 60

Asp Val Tyr Gly Pro Phe Pro Asp Asp Arg Gly Trp Asp Val Glu Gly

65 70 75 80

Leu Tyr His Pro Asp Pro Asp His Pro Gly Thr Thr Tyr Val Arg Glu

85 90 95

Gly Ala Phe Leu His Asp Ala Ala Arg Phe Asp Ala Gly Phe Phe Gly

100 105 110

Ile Ser Pro Arg Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Gln Leu

115 120 125

Leu Glu Val Ser Trp Glu Thr Phe Glu Arg Ala Gly Ile Asp Pro His

130 135 140

Ala Met Arg Gly Gly Asp Val Gly Val Phe Ala Gly Ile Val His Gln

145 150 155 160

Asp Tyr Ala Pro Asp Leu Ser Gly Phe Glu Gly Tyr Leu Ser Leu Glu

165 170 175

Arg Ala Leu Gly Thr Ala Gly Gly Val Ala Ser Gly Arg Val Ala Tyr

180 185 190

Thr Leu Gly Leu Glu Gly Pro Ala Val Thr Val Asp Thr Met Cys Ser

195 200 205

Ser Ser Leu Val Ala Ile His Leu Ala Ala Gln Ala Leu Arg Arg Gly

210 215 220

Glu Cys Thr Met Ala Leu Ala Gly Gly Ser Thr Val Met Ala Thr Pro

225 230 235 240

Gly Gly Phe Val Gly Phe Ala Arg Gln Arg Ala Leu Ala Phe Asp Gly

245 250 255

Arg Cys Lys Ser Tyr Ala Ala Gly Ala Asp Gly Ser Gly Trp Ala Glu

260 265 270

Gly Val Gly Val Leu Leu Leu Glu Arg Leu Ser Val Ala Arg Glu Arg

275 280 285

Gly His Gln Val Leu Ala Val Ile Arg Gly Ser Ala Val Asn Gln Asp

290 295 300

Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ala Gln Gln Arg

305 310 315 320

Val Ile Arg Lys Ala Leu Ala Gly Ala Gly Leu Ala Pro Ser Asp Val

325 330 335

Asp Ala Val Glu Gly His Gly Thr Gly Thr Val Leu Gly Asp Pro Ile

340 345 350

Glu Ala Gln Ala Leu Met Ala Thr Tyr Gly Gln Gly Arg Asp Pro Glu

355 360 365

ArgPro Leu Trp Leu Gly Ser Val Lys Ser Val Ile Gly His Thr Gln

370 375 380

Ala Ala Ser Gly Val Ala Gly Val Ile Lys Met Val Gln Ala Leu Arg

385 390 395 400

His Gly Val Leu Pro Ala Thr Leu His Val Asp Ala Pro Thr Pro Gln

405 410 415

Val Asp Trp Ser Ala Gly Ala Val Glu Leu Leu Thr Glu Ala Arg Gly

420 425 430

Trp Glu Arg Asn Gly Arg Ala Arg Arg Ala Gly Val Ser Ala Phe Gly

435 440 445

Ala Ser Gly Thr Asn Ala His Leu Ile Leu Glu Glu Ala Pro Glu Ala

450 455 460

Pro Val Thr Glu Glu Ser Ala Pro Ser Gly Val Val Pro Leu Val Val

465 470 475 480

Ser Ala Gly Thr Ala Ala Ser Leu Thr Glu Gln Ala Glu Arg Leu Ala

485 490 495

Ser Tyr Leu Glu Ser Gly Thr Asp Gly Ala Ser Leu Ala Ala Val Ala

500 505 510

Gly Thr Leu Ala Ala Gly Arg Ala Val Leu Gly Glu Arg Ala Val Val

515 520 525

Val Ala GlyThr Thr Asp Glu Ala Leu Thr Gly Leu Arg Ala Leu Ala

530 535 540

Arg Gly Glu Asn Ala Thr Gly Leu Val Thr Gly Thr Gly Thr Pro Gly

545 550 555 560

Lys Val Val Trp Val Phe Pro Gly Gln Gly Ser Gln Trp Val Gly Met

565 570 575

Gly Arg Glu Leu Leu Asp Ser Ser Pro Val Phe Val Glu Arg Val Ala

580 585 590

Glu Cys Ala Ala Ala Leu Glu Arg Trp Val Asp Trp Ser Leu Val Asp

595 600 605

Val Leu Arg Gly Glu Val Glu Pro Ala Leu Leu Glu Arg Val Asp Val

610 615 620

Val Gln Pro Ala Ser Phe Ala Val Met Val Gly Leu Ala Ala Val Trp

625 630 635 640

Ala Ser Val Gly Val Glu Pro Asp Ala Val Leu Gly His Ser Gln Gly

645 650 655

Glu Ile Ala Ala Ala Cys Val Ala Gly Ala Leu Ser Leu Glu Asp Ala

660 665 670

Ala Arg Val Val Ala Leu Arg Ser Gln Ala Ile Ala Ala Arg Leu Ala

675 680 685

Gly Arg Gly Gly MetAla Ser Val Ala Leu Ser Glu Glu Asp Ala Ala

690 695 700

Gly Arg Leu Gly Pro Trp Ser Asp Arg Val Glu Val Ala Ala Val Asn

705 710 715 720

Gly Pro Ser Ser Val Val Val Ala Gly Asp Ala Gln Ala Leu Asp Glu

725 730 735

Ala Leu Glu Ala Leu Asp Asp Gln Gly Val Arg Ile Arg Arg Ile Ala

740 745 750

Val Asp Tyr Ala Ser His Thr Arg His Val Glu Asp Val Arg Thr Thr

755 760 765

Leu Ala Glu Val Leu Ala Gly Ile Gly Ala Gln Glu Pro Thr Val Pro

770 775 780

Phe Tyr Ser Thr Val Thr Gly Gly Trp Val Thr Asp Ala Gly Ala Leu

785 790 795 800

Asp Ser Ala Tyr Trp Tyr Arg Asn Leu Arg Glu Arg Val Arg Phe Gly

805 810 815

Pro Ala Val Ala Asp Leu Leu Asp Gln Gly His Gly Val Phe Val Glu

820 825 830

Val Ser Ala His Pro Val Leu Val Gln Pro Ile Thr Glu Ala Val Val

835 840 845

Ala Ala Asp Ala Asp Val ValVal Thr Gly Ser Leu Arg Arg Glu Asp

850 855 860

Gly Gly Leu Arg Arg Leu Leu Thr Ser Met Ala Glu Leu Phe Val Arg

865 870 875 880

Gly Thr Pro Leu Asp Trp Ala Arg Ala Leu Pro Ala Gly Ala Thr Ser

885 890 895

Arg Arg Val Asp Leu Pro Thr Tyr Ala Phe Asp His Gln His Tyr Trp

900 905 910

Ile Arg Met Gly Ala Ser Ala Thr Asp Ser Ala Ser Leu Gly Leu Ala

915 920 925

Gly Ala Asp His Pro Leu Leu Gly Ala Val Val Pro Leu Pro Gln Ser

930 935 940

Asp Gly Leu Val Phe Thr Ser Arg Leu Ser Leu Arg Thr His Pro Trp

945 950 955 960

Leu Ala Asp His Glu Ile Gly Gly Ala Val Leu Val Pro Gly Thr Val

965 970 975

Tyr Val Asp Leu Ala Val Arg Ala Gly Asp Glu Phe Gly His Gly Leu

980 985 990

Leu Glu Glu Leu Val Ile Glu Ala Pro Leu Val Leu Pro Asp Ser Gly

995 1000 1005

Gly Val Arg Leu Gln Val Ala Val SerGly Pro Gly Ala Thr Gly

1010 1015 1020

Ser Arg Thr Val Asp Val Tyr Ser Gln Arg Glu Asp Ala Ala Asp

1025 1030 1035

Asp Gly Gly Ala Asp Asp Val Trp Thr Arg His Ala Thr Gly Leu

1040 1045 1050

Leu Ala Ala Ser Pro Thr Ala Pro Asp Pro Gly Arg Ala Phe Asp

1055 1060 1065

Phe Ala Ala Trp Pro Pro Pro Gly Ala Glu Pro Val Pro Met Thr

1070 1075 1080

Asp Phe Tyr Ala Asp Leu Val Glu Arg Gly Tyr Ala Tyr Gly Pro

1085 1090 1095

Ala Phe Gln Gly Leu Arg Ala Val Trp Arg Arg Gly Asp Glu Val

1100 1105 1110

Phe Ala Glu Ala Ala Leu Pro Glu Glu His Arg Glu Glu Ala Gly

1115 1120 1125

Gly Phe Gly Ile His Pro Ala Leu Leu Asp Ala Ala Leu His Thr

1130 1135 1140

Asn Ala Phe Ala Arg Pro Asp Asp Asp Arg Lys Val Leu Pro Phe

1145 1150 1155

Ala Trp Asn Gly Leu Leu Leu His Ala Val Gly Ala Ser Ala Leu

1160 1165 1170

Arg Val Arg Val Ala Pro Cys Gly Pro Asp Ala Leu Ser Phe His

1175 1180 1185

Ala Ala Asp Asp Thr Gly Ala Thr Val Leu Thr Met Asp Ser Leu

1190 1195 1200

Val Ser Leu Pro Val Ser Thr Asp Gln Leu Asp Ala Ala Ala Ala

1205 1210 1215

Asp Gly Ser Arg Asp Thr Leu Phe Gly Val Glu Trp Thr Glu Leu

1220 1225 1230

Pro Pro Val Arg Gly Asp Val Ala Gly Ser Ala Thr Ala Pro Pro

1235 1240 1245

Leu Ala Val Leu Glu Ala Phe Gly Asp Asp Gly Glu Gly Ala Val

1250 1255 1260

Leu Ala Leu Thr Thr Arg Val Leu Gly Ala Val Gln Glu Trp Leu

1265 1270 1275

Ala Gly Ala Gly Ser Asp Asp Ser Arg Leu Val Val Val Thr Arg

1280 1285 1290

Gly Ala Val Pro Ala Gly Asp Gly Ala Val Thr Asp Pro Ala Gly

1295 1300 1305

Ala Ala Val Trp Gly Leu Val Arg Ala Ala Gln Ala Glu Asn Pro

1310 1315 1320

Glu Arg Ile Leu Leu Val Asp Thr Asp Pro Ala Ala Gly Gly Asp

1325 1330 1335

Leu Asp Ala Ala Leu Gly Val Ala Leu Ala Ser Gly Glu Pro Gln

1340 1345 1350

Leu Ala Val Arg Gly Ala Ile Phe Ser Val Pro Arg Leu Val Arg

1355 1360 1365

Val Ala Gly Arg Gly Leu Gly Ser Gly Asn Val Phe Ala Ala Gly

1370 1375 1380

Lys Thr Val Leu Leu Ser Gly Gly Thr Gly Ser Leu Gly Gly Leu

1385 1390 1395

Val Ala Arg His Leu Val Ala Arg His Gly Val Arg His Leu Leu

1400 1405 1410

Leu Ala Ser Arg Arg Gly Ala Asp Ala Glu Gly Met Pro Glu Leu

1415 1420 1425

Val Ala Glu Leu Ala Glu Gln Gly Ala Val Val Ser Val Val Ala

1430 1435 1440

Cys Asp Val Ser Asp Arg Asp Gln Val Arg Ala Leu Leu Ala Ala

1445 1450 1455

Val Pro Asp Glu His Pro Leu Thr Gly Ile Val His Met Ala Gly

1460 1465 1470

Val Leu Asp Asp Gly Val Ile Gly Thr Leu Thr Pro Glu Arg Leu

1475 1480 1485

Ala Gly Val Phe Ala Pro Lys Val Asp Ala Val Ser His Leu Asp

1490 1495 1500

Glu Leu Thr Arg Glu Leu Gly Leu Glu Leu Asp Ala Phe Val Val

1505 1510 1515

Phe Ser Ser Ala Ala Ala Leu Met Gly Ser Ala Gly Gln Gly Asn

1520 1525 1530

Tyr Ala Ala Ala Asn Ala Tyr Leu Asp Gly Leu Met Ser Gly Arg

1535 1540 1545

Arg Ala Ala Gly Leu Pro Gly Ile Ser Leu Ala Trp Gly Leu Trp

1550 1555 1560

Glu Gln Thr Ala Gly Leu Thr Ala His Leu Ser Ser Val Asp Gln

1565 1570 1575

Ala Arg Met Ser Arg Gly Gly Val Leu Ala Met Thr Pro Ala Glu

1580 1585 1590

Gly Leu Ala Val Phe Asp Ile Gly Leu His Ala Gly Gln Ala Leu

1595 1600 1605

Leu Val Pro Ile Lys Leu Asp Leu Arg Thr Leu Arg Thr Gln Ala

1610 1615 1620

Ala Ala Gly Gly Gly Val Pro Gln Leu Leu Arg Gly Leu Val Arg

1625 1630 1635

Ala Gly Arg Arg Val Val Arg Ala Ala Ala Gly Asp Ser Gly Gly

16401645 1650

Leu Val Arg Arg Leu Ala Gly Leu Pro Pro Ala Glu Gln Glu Asn

1655 1660 1665

Leu Leu Leu Ser Leu Val Gln Gly Glu Ala Gly Gly Val Leu Gly

1670 1675 1680

Phe Ser Gly Pro Glu Leu Thr Gln Gly Thr Arg Gly Phe Ser Asp

1685 1690 1695

Ile Gly Phe Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu

1700 1705 1710

Ser Ala Ala Thr Gly Val Lys Leu Pro Ala Thr Leu Ile Phe Asp

1715 1720 1725

Tyr Pro Thr Pro Val Ala Leu Ala Arg His Leu Arg Glu Glu Leu

1730 1735 1740

Gly Asp Thr Ala Asp Gly Ala Ala Ala Pro Ala Ala Val Ala Val

1745 1750 1755

Ala Asp Pro Asp Glu Pro Ile Ala Ile Val Gly Met Ala Cys Arg

1760 1765 1770

Leu Pro Gly Gly Val Thr Asp Pro Asp Gly Leu Trp Arg Leu Val

1775 1780 1785

Arg Glu Gly Arg Glu Gly Met Ser Asp Phe Pro Asp Asp Arg Gly

1790 1795 1800

Trp Asp Leu Glu Gly Leu Phe Asp Ala Asp ProAsp Asn Ala Gly

1805 1810 1815

Thr Ser Tyr Ala Ser Gln Gly Gly Phe Leu Glu Gly Ala Gly Leu

1820 1825 1830

Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala Leu Ala

1835 1840 1845

Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Ala

1850 1855 1860

Leu Glu Arg Ala Gly Ile Asp Pro Thr Thr Ala Arg Gly Ala Asp

1865 1870 1875

Ile Gly Val Phe Ser Gly Val Ser Ile His Asp Tyr Leu Glu Ser

1880 1885 1890

Leu Ser Asn Met Pro Asp Glu Leu Glu Gly Phe Val Thr Thr Ala

1895 1900 1905

Thr Ala Gly Ser Val Ala Ser Gly Arg Val Ser Tyr Ala Phe Gly

1910 1915 1920

Phe Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser

1925 1930 1935

Leu Val Ala Met His Leu Ala Ala Gln Ala Leu Arg Gln Gly Glu

1940 1945 1950

Cys Ser Met Ala Leu Ala Gly Gly Val Ala Val Met Gly Ser Pro

1955 1960 1965

Ile Gly Val Leu Gly Met Ser Arg Gln Arg Gly Leu Ala Ala Asp

1970 1975 1980

Gly Arg Cys Lys Ala Tyr Ala Asp Gly Ala Asp Gly Thr Val Leu

1985 1990 1995

Ser Glu Gly Val Gly Leu Val Val Leu Glu Arg Leu Ser Val Ala

2000 2005 2010

Arg Glu Arg Gly His Arg Val Leu Ala Val Ile Arg Gly Ser Ala

2015 2020 2025

Val Asn Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly

2030 2035 2040

Pro Ser Gln Gln Arg Val Ile Arg Lys Ala Leu Ala Asn Ala Gly

2045 2050 2055

Leu Ser Pro Ser Glu Val Asp Val Val Glu Gly His Gly Thr Gly

2060 2065 2070

Thr Thr Leu Gly Asp Pro Ile Glu Thr Gln Ala Leu Leu Ala Thr

2075 2080 2085

Tyr Gly Lys Gly Arg Asp Ala Glu Gln Pro Leu Arg Leu Gly Ser

2090 2095 2100

Leu Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala Gly Val Ser

2105 2110 2115

Ser Val Ile Lys Met Val Gln Ala Leu Arg His Asn Val Met Pro

2120 2125 2130

Pro Thr Leu His Ala Gln Glu Pro Thr His Gln Val Asp Trp Ser

2135 2140 2145

Ala Gly Ala Ile Glu Leu Leu Thr Glu Pro Arg Asp Trp Pro Arg

2150 2155 2160

Asn Gly Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Ile Ser

2165 2170 2175

Gly Thr Asn Ala His Leu Ile Leu Glu Glu Ala Pro Glu Glu Pro

2180 2185 2190

Leu Ser Glu Glu Pro Ala Ser Val Gly Val Val Pro Val Val Val

2195 2200 2205

Ser Ala Arg Ser Ala Ala Ser Leu Ala Gly Gln Ala Gly Arg Leu

2210 2215 2220

Ala Ser Tyr Val Glu Ser Gly Gly Gly Ala Ser Leu Ala Gly Val

2225 2230 2235

Ala Gly Ala Leu Val Thr Ser Arg Ala Ala Leu Asp Glu Arg Ala

2240 2245 2250

Val Val Val Ala Gly Ser Asn Ala Glu Ala Leu Gly Gly Leu Arg

2255 2260 2265

Ala Leu Ala Arg Gly Glu Asp Ala Ala Gly Val Val Ser Gly Lys

2270 2275 2280

Val Ala Gly Ser Gly Val Pro Gly Lys Val Val Trp Val Phe Pro

2285 2290 2295

Gly Gln Gly Ala Gln Trp Ala Gly Met Gly Arg Glu Leu Leu Asp

2300 2305 2310

Thr Ser Thr Val Phe Ala Glu Arg Ile Ala Glu Cys Ala Ala Ala

2315 2320 2325

Leu Glu Ser Leu Val Asp Trp Ser Leu Val Asp Val Leu Arg Gly

2330 2335 2340

Glu Val Glu Pro Ala Leu Leu Glu Arg Val Asp Val Val Gln Pro

2345 2350 2355

Ala Ser Phe Ala Val Met Val Gly Leu Ala Ser Val Trp Ala Ser

2360 2365 2370

Val Gly Val Lys Pro Asp Ala Val Leu Gly His Ser Gln Gly Glu

2375 2380 2385

Ile Ala Ala Ala Cys Val Ala Gly Ala Leu Ser Leu Glu Asp Ala

2390 2395 2400

Val Arg Val Val Ala Leu Arg Ser Gln Ala Ile Ala Thr Glu Leu

2405 2410 2415

Ala Gly Arg Gly Gly Met Ala Ser Val Ala Leu Ser Ala Asp Glu

2420 2425 2430

Ala Ala Ala Arg Leu Glu Glu Trp Ala Gly Arg Val Glu Val Ala

2435 2440 2445

Ala Val Asn Gly Pro Ser Ser Val Val Val Ala Gly Asp Ala Glu

2450 2455 2460

Ala Leu Asp Glu Ala Leu Glu Ala Leu Ala Gly Asp Gly Val Arg

2465 2470 2475

Thr Arg Arg Val Ala Val Asp Tyr Ala Ser His Thr Arg His Val

2480 2485 2490

Glu Asp Ile Arg Asp Arg Leu Ala Glu Ala Leu Ala Gly Val Ser

2495 2500 2505

Ala Glu Ala Pro Val Val Pro Phe Tyr Ser Thr Val Thr Gly Ser

2510 2515 2520

Trp Val Glu Asp Ala Gly Val Leu Asp Gly Gly Tyr Trp Tyr Arg

2525 2530 2535

Asn Leu Arg Gly Gln Val Gly Phe Gly Pro Ala Val Ala Asp Leu

2540 2545 2550

Leu Ala Gln Gly His Gly Val Phe Val Glu Val Ser Ala His Pro

2555 2560 2565

Val Leu Val Gln Pro Val Ser Glu Ala Val Asp Glu Ser Gly Ala

2570 2575 2580

Asp Ala Val Val Thr Gly Ser Leu Arg Arg Asp Glu Gly Gly Leu

2585 2590 2595

Arg Arg Leu Leu Met Ser Met Ala Glu Leu Phe Val ArgGly Val

2600 2605 2610

Ala Val Gly Trp Ser Ala Val Leu Pro Gly Gly Ala Gly Val Glu

2615 2620 2625

Ile Ala Arg Val Glu Leu Pro Thr Tyr Ala Phe Asp His Gln His

2630 2635 2640

Tyr Trp Leu Lys Thr Ala Ala Gln Val Thr Asp Ala Ala Ser Leu

2645 2650 2655

Gly Gln Ala Ala Ala Asp His Pro Leu Leu Gly Ala Val Val Arg

2660 2665 2670

Leu Pro Gln Ser Asp Gly Leu Val Phe Thr Ser Arg Leu Ser Leu

2675 2680 2685

Arg Thr His Ala Trp Leu Ala Asp His Arg Val Gly Asp Val Val

2690 2695 2700

Leu Val Pro Gly Thr Ser Leu Val Glu Leu Ala Val Arg Ala Gly

2705 2710 2715

Asp Glu Ala Gly Cys Gly Val Leu Asp Glu Leu Val Ile Gly Glu

2720 2725 2730

Pro Leu Val Val Pro Glu Arg Gly Gly Val Arg Val Gln Val Ala

2735 2740 2745

Val Gly Gly Pro Gly Glu Asn Gly Ala Arg Thr Val Glu Val Tyr

2750 2755 2760

Ser Gln Arg Glu Asp Ala Gly Asp Glu Ala Asp Ala Trp Thr Arg

2765 2770 2775

His Ala Thr Gly Val Leu Ser Ala Ala Thr Asp Arg Ser Thr Gly

2780 2785 2790

Ser Gly Thr Gly Phe Asp Phe Thr Ala Trp Pro Pro Pro Gly Ala

2795 2800 2805

Glu Pro Val Gly Ile Gly Glu Phe Tyr Ala Asp Leu Ala Glu Gln

2810 2815 2820

Gly Tyr Ala Tyr Gly Pro Ala Phe Gln Gly Val Arg Ala Val Trp

2825 2830 2835

Arg Arg Gly Glu Glu Leu Phe Ala Glu Val Ala Leu Pro Glu Glu

2840 2845 2850

Gln His Lys Glu Ala Gly Arg Phe Gly Ile His Pro Ala Leu Leu

2855 2860 2865

Asp Ala Ala Leu His Ala Gly Glu Pro Gly Ala Ala Ala Glu Gly

2870 2875 2880

Val Lys Glu Leu Arg Gln Pro Leu Asp Trp Asn Gly Leu Val Leu

2885 2890 2895

His Ala Ala Gly Ala Ser Ala Leu Arg Ala Arg Leu Val Pro Ala

2900 2905 2910

Gly Pro Asp Ala Val Ser Leu Glu Ala Ala Asp Glu Ala Gly Gly

2915 2920 2925

Leu Val Val Thr Ala Glu Ser Leu Thr Trp Arg Glu Val Ser Thr

2930 2935 2940

Asp Arg Pro Gly Ala Ala Asp Thr Leu Val Ala Gln Ser Leu Phe

2945 2950 2955

Gly Val Val Trp Thr Glu Leu Pro Pro Thr Gln Gly Ala Ile Thr

2960 2965 2970

Ser Leu Ser Trp Met Pro Val Ala Ala Ala Asp Glu Val Ala Thr

2975 2980 2985

Leu Thr Asp Glu Val Ala Ala Gly Ala Ala Ala Pro Met Ala Ala

2990 2995 3000

Val Leu Glu Ala Phe Gly Gly Gly Asp Gly Glu Glu Leu Ala Leu

3005 3010 3015

Ser Ser His Val Leu Gly Val Val Gln Gly Trp Leu Asn Gly Ala

3020 3025 3030

Gly Leu Glu Glu Ser Arg Leu Val Val Val Thr Arg Gly Ala Val

3035 3040 3045

Pro Ala Gly Glu Gly Ala Val Thr Asp Pro Ala Ala Ala Ala Val

3050 3055 3060

Trp Gly Leu Val Arg Ala Ala Gln Ala Glu Asn Pro Asp Arg Ile

3065 3070 3075

Val Leu Leu AspLeu Asp Pro Ala Ser Asp Asp Ala Ala Gly Ser

3080 3085 3090

Val Leu Asp Thr Val Leu Gly Ala Val Leu Ala Thr Gly Glu Pro

3095 3100 3105

Gln Ile Ala Val Arg Gly Thr Ala Phe Ser Ala Pro Arg Leu Val

3110 3115 3120

Arg Val Pro Ala Gln Ala Pro Asp Ser Gln Ser Val Phe Thr Ser

3125 3130 3135

Glu Gly Thr Val Leu Ile Thr Gly Gly Thr Gly Ser Leu Gly Ala

3140 3145 3150

Leu Val Ala Arg His Leu Val Thr Arg Tyr Gly Val Arg Arg Leu

3155 3160 3165

Leu Leu Ala Ser Arg Arg Gly Arg Asp Ala Glu Gly Ala Pro Glu

3170 3175 3180

Leu Ala Ala Glu Leu Ala Glu Leu Gly Ala Thr Val Ser Val Val

3185 3190 3195

Ala Cys Asp Val Ser Asp Arg Asp Gln Ala Lys Ala Leu Leu Ala

3200 3205 3210

Ser Val Ser Asp Asp His Glu His Arg Leu Thr Gly Ile Val His

3215 3220 3225

Thr Ala Gly Val Leu Asp Ala Gly Val Ile Gly Ala Leu Thr Pro

3230 32353240

Glu Arg Leu Glu Arg Val Phe Ala Pro Lys Val Asp Ala Val Arg

3245 3250 3255

His Leu Asp Glu Leu Thr Arg Glu Leu Gly Leu Asp Leu Asp Ala

3260 3265 3270

Phe Val Val Tyr Ser Ser Val Ser Gly Val Phe Met Gly Ala Gly

3275 3280 3285

Ser Gly Ser Tyr Ala Ala Ala Asn Ala Tyr Leu Asp Gly Leu Met

3290 3295 3300

Ala Asn Arg Arg Ala Ala Gly Leu His Gly Leu Ser Leu Ala Trp

3305 3310 3315

Gly Leu Trp Ala Gln Thr Ser Gly Met Ala Ala Asn Thr Asp Asp

3320 3325 3330

Leu Thr Arg Asn Arg Met Asn Arg Arg Gly Gly Leu Gln Ala Met

3335 3340 3345

Ser Leu Thr Glu Gly Met Glu Leu Phe Asp Ala Ala Val Gly Thr

3350 3355 3360

Arg Gln Ser Leu Leu Val Pro Ala Arg Leu Asp Leu Arg Gly Val

3365 3370 3375

Arg Ala Asp Ala Ala Ala Gly Gly Gly Val Pro His Met Leu Arg

3380 3385 3390

Gly Leu Val Arg Ala Gly Arg Gln Ala Ala Arg Ala Ala Asp Gly

3395 3400 3405

Gly Asp Lys Arg Arg Arg Leu Thr Asp Arg Leu Thr Gly Leu Ala

3410 3415 3420

Pro Ala Glu Gln Glu Ala Leu Leu Leu Asp Leu Val Arg Ala Arg

3425 3430 3435

Ala Ala Val Val Leu Gly His Ala Gly Pro Ser Gly Val Gln Pro

3440 3445 3450

Asp Met Ala Phe Asn Glu Val Gly Phe Asp Ser Leu Thr Ala Val

3455 3460 3465

Glu Leu Arg Asn Arg Leu Arg Glu Ala Thr Gly Leu Lys Leu Pro

3470 3475 3480

Ala Thr Leu Ile Phe Asp Tyr Pro Asn Pro Leu Ala Leu Ala Arg

3485 3490 3495

Tyr Leu His Arg Glu Leu Leu Pro Asp Asp Gly Ala Asn Gly Gln

3500 3505 3510

Glu Leu Asp Glu Ala Arg Leu Arg Arg Ala Leu Ala Ser Leu Pro

3515 3520 3525

Leu Ala Arg Phe Arg Ala Ala Gly Leu Met Asp Ala Leu Val Glu

3530 3535 3540

Leu Ala Ala Leu Ala Asp Gly Ala Pro His Thr Arg Thr Thr Asp

3545 3550 3555

Glu Asn Ser Glu Ala Glu Gly Gly Asp Glu Lys Arg Ala Ile Ala

3560 3565 3570

Glu Leu Asp Val Asp Asp Leu Val His Leu Ala Leu Gly Asp

3575 3580 3585

<210>12

<211>3570

<212>PRT

<213>Streptomyces spectabilis

<400>12

Leu Trp Arg Leu Val Ala Glu Gly Arg Asp Ala Val Ser Gly Phe Pro

1 5 10 15

Glu Asp Arg Gly Trp Asp Leu Glu Gly Leu Phe Ala Pro Asp Pro Glu

20 25 30

Ser Ala Gly Thr Ser Tyr Thr Arg Gln Gly Gly Phe Leu His Glu Ala

35 40 45

Gly Leu Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala Met

50 55 60

Ala Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Ala

65 70 75 80

Leu Glu Ala Ala Gly Met Asp Pro Gly Ser Leu Lys Gly Ala Asp Val

85 90 95

Gly Val Phe Ser Gly Val Phe Thr Gln Gly Tyr Val Ala Pro Gly Ala

100 105 110

Ser Gly Val Thr Pro Glu Val Glu Gly Phe Ala Gly Thr Gly Gly Ser

115 120 125

Thr Ser Val Ala Ser Gly Arg Val Ser Tyr Val Leu Gly Phe Glu Gly

130 135 140

Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val Ala Met

145 150 155 160

His Met Ala Ala Gln Ala Leu Arg Gln Gly Glu Cys Ser Met Ala Leu

165 170 175

Ala Gly Gly Ala Thr Val Met Ala Thr Pro Gly Ala Phe Val Glu Phe

180 185 190

Ser Arg Gln Arg Gly Leu Ala Ala Asp Gly Arg Cys Lys Ala Phe Ser

195 200 205

Ser Ser Ala Asp Gly Thr Gly Trp Ala Glu Gly Val Gly Val Val Val

210 215 220

Leu Glu Arg Leu Ser Val Ala Arg Glu Arg Gly His Arg Ile Leu Ala

225 230 235 240

Val Leu Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly Leu

245 250 255

Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Gly Ala Leu

260265 270

Ala Asn Ala Gly Leu Ser Pro Ser Glu Val Asp Val Val Glu Gly His

275 280 285

Gly Thr Gly Thr Thr Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu

290 295 300

Ala Thr Tyr Gly Arg Gly Arg Asp Pro Glu Gln Pro Leu Trp Leu Gly

305 310 315 320

Ser Leu Lys Ser Asn Ile Gly His Ala Gln Ala Ala Ala Gly Val Ala

325 330 335

Gly Val Ile Lys Met Val Gln Ala Leu Arg His Gly Val Leu Pro Ala

340 345 350

Thr Leu His Val Ala Ala Pro Thr Pro Gln Val Asp Trp Ser Ala Gly

355 360 365

Ala Val Glu Leu Leu Thr Glu Thr Arg Glu Trp Pro Arg Asp Gly Arg

370 375 380

Pro Arg Arg Ala Gly Val Ser Ala Phe Gly Met Ser Gly Thr Asn Ala

385 390 395 400

His Leu Ile Leu Glu Glu Ala Pro Glu Gln Pro Leu Pro Glu His Ala

405 410 415

Val Pro Ala Gly Val Val Pro Leu Val Val Ser Ala Arg Ser Gln Thr

420425 430

Ser Leu Thr Gly Gln Ala Glu Arg Leu Ile Thr His Leu Glu Ser Ala

435 440 445

Pro Asp Gly Thr Ala Pro Leu Ala Pro Val Ala Gly Ala Leu Val Ser

450 455 460

Gly Arg Gly Val Phe Gly Glu Arg Ala Val Val Val Ala Gly Ser Glu

465 470 475 480

Gly Glu Ala Val Ser Gly Leu Arg Ala Leu Ala Arg Gly Glu Ser Asp

485 490 495

Val Asn Leu Val Ser Gly Ser Gly Gly Val Pro Gly Lys Val Val Trp

500 505 510

Val Phe Pro Gly Gln Gly Ser Gln Trp Ala Gly Met Gly Arg Glu Leu

515 520 525

Leu Asp Ser Ser Pro Val Phe Ala Glu Arg Val Gly Glu Cys Ala Ala

530 535 540

Ala Leu Gly Pro Trp Val Asp Trp Ser Leu Val Asp Val Leu Arg Gly

545 550 555 560

Asp Val Glu Pro Ala Leu Leu Glu Arg Val Asp Val Val Gln Pro Ala

565 570 575

Ser Phe Ala Val Met Val Gly Leu Ala Ser Val Trp Ala Ser Val Gly

580 585590

Val Lys Pro Asp Ala Val Val Gly His Ser Gln Gly Glu Ile Ala Ala

595 600 605

Ala Cys Val Ala Gly Ala Leu Ser Leu Glu Asp Ala Val Arg Val Val

610 615 620

Ala Leu Arg Ser Gln Ala Ile Ala Ala Arg Leu Ala Gly Arg Gly Gly

625 630 635 640

Met Ala Ser Val Ala Leu Ala Glu Gln Glu Val Ala Gly Arg Leu Glu

645 650 655

Pro Trp Ala Glu Arg Val Glu Val Ala Ala Val Asn Gly Pro Ser Ser

660 665 670

Val Val Ile Ala Gly Asp Ala Glu Ala Leu Asp Glu Val Leu Gln Val

675 680 685

Leu Ser Asp Asp Gly Val Arg Val Arg Arg Val Ala Val Asp Tyr Ala

690 695 700

Ser His Thr Arg His Val Glu Asp Ile Gln Asp Thr Leu Ala Glu Ala

705 710 715 720

Leu Ala Gly Val Ser Ala Gln Ala Pro Ala Val Pro Phe Tyr Ser Thr

725 730 735

Val Thr Gly Ser Trp Val Glu Asp Ala Gly Val Leu Asp Gly Gly Tyr

740 745750

Trp Tyr Arg Asn Leu Arg Gly Gln Val Gly Phe Gly Pro Ala Val Ala

755 760 765

Asp Leu Leu Ala Gln Gly His Gly Val Phe Val Glu Val Ser Ala His

770 775 780

Pro Val Leu Val Gln Pro Val Ser Glu Ile Val Asp Glu Ser Gly Ala

785 790 795 800

Asp Ala Val Val Thr Gly Ser Leu Arg Arg Asp Glu Gly Gly Leu Arg

805 810 815

Arg Leu Leu Val Ser Met Ala Glu Leu Phe Val Arg Gly Val Ala Val

820 825 830

Asp Trp Ala Ala Val Leu Pro Gly Gly Ala Gly Gly Asp Thr Ala Arg

835 840 845

Val Glu Leu Pro Thr Tyr Ala Phe Asp His Arg His Tyr Trp Leu Lys

850 855 860

Gln Gly Ala Ser Ala Thr Asp Ala Ala Ser Leu Gly Gln Ala Ala Ala

865 870 875 880

Asp His Pro Leu Leu Gly Ala Val Val Arg Leu Pro Gln Ser Asp Gly

885 890 895

Leu Val Phe Thr Ser Arg Leu Ser Leu Arg Thr His Ala Trp Leu Ala

900 905910

Asp His Arg Val Gly Asp Val Val Leu Val Pro Gly Thr Gly Leu Val

915 920 925

Glu Leu Ala Val Arg Ala Gly Asp Glu Ala Gly Cys Gly Val Leu Glu

930 935 940

Glu Leu Val Val Glu Ala Pro Leu Val Val Pro Glu Arg Gly Gly Val

945 950 955 960

Arg Val Gln Val Ser Leu Gly Gly Pro Gly Glu Asn Gly Val Arg Thr

965 970 975

Val Glu Val Tyr Ser Gln Arg Glu Asp Ala Gly Asp Glu Ala Asp Ala

980 985 990

Trp Thr Arg His Ala Thr Gly Leu Leu Ser Ala Ala Ala Arg Pro Asp

995 1000 1005

Gly Ser Ser Gly Gly Ser Ser Gly Gly Gly Gly Ser Ser Val Asp

1010 1015 1020

Phe Thr Ala Trp Pro Pro Arg Gly Ala Gln Pro Val Glu Val Gly

1025 1030 1035

Asp Phe Tyr Glu Ala Leu Ala Glu Arg Gly Tyr Gly Tyr Gly Pro

1040 1045 1050

Ala Phe Gln Gly Val Arg Ala Val Trp Arg Arg Gly Asp Glu Val

1055 1060 1065

Phe Ala Glu Val Val Leu Pro Glu Glu Gln Arg Lys Glu Ala Gly

1070 1075 1080

Arg Tyr Gly Ile His Pro Ala Leu Leu Asp Ala Ala Leu Gln Ala

1085 1090 1095

Gly Thr Ile Gly Ala Ala Ala Gly Pro Ala Val Glu Gly Ser Gly

1100 1105 1110

Glu Pro Val Leu Ala Phe Ala Trp Asn Gly Leu Val Leu His Ala

1115 1120 1125

Gly Gly Ala Ser Ala Leu Arg Val Arg Val Ala Pro Ser Gly Pro

1130 1135 1140

Asp Ala Leu Ser Val Glu Ala Ala Asp Glu Ala Gly Gly Leu Val

1145 1150 1155

Val Thr Met Asp Ser Leu Val Ser Arg Pro Val Ser Ala Asp Gln

1160 1165 1170

Leu Gly Ala Ala Thr Ala Ala His Ser Leu Phe Gln Val Glu Trp

1175 1180 1185

Ala Glu Val Ser Gln Glu Pro Glu Pro Ser Pro Leu Trp Ala Pro

1190 1195 1200

Val Thr Thr Pro Asp Asp Val Ala Leu Leu Pro Gly Asn Gly Pro

1205 1210 1215

Ala Val Ala Val Met Glu Ala Leu Gly Gly Asp Glu Glu Asp Ala

1220 1225 1230

Val Leu Thr Leu Thr Ser Gln Val Leu Ala Val Leu Gln Ala Trp

1235 1240 1245

Leu Ala Glu Ala Ala Leu Glu Glu Ser Arg Leu Val Val Val Thr

1250 1255 1260

Arg Gly Ala Val Ala Ala Gly Gly Glu Asp Met Val Thr Asp Pro

1265 1270 1275

Ala Ala Ala Ala Val Trp Gly Leu Val Arg Ala Ala Gln Ser Glu

1280 1285 1290

Asn Pro Asp Arg Ile Val Leu Leu Asp Thr Asp Pro Thr Ala Asp

1295 1300 1305

Thr Ala Pro Asp Thr Thr Pro Asp Thr Ser Pro Asp Ala Val Leu

1310 1315 1320

Gly Ala Val Leu Ala Cys Gly Glu Pro Gln Val Ala Val Arg Gly

1325 1330 1335

Gly Ala Leu Leu Leu Pro Arg Leu Gly Arg Val Ala Gly His Val

1340 1345 1350

Thr Asp Gly Pro Ala Val Phe Gly Pro Glu Gly Thr Val Leu Ile

1355 1360 1365

Ser Gly Ala Gly Ser Leu Gly Ala Leu Val Ala Arg His Leu Val

1370 1375 1380

Thr Arg His Gly Val Arg Ser Leu Leu Leu Ala Ser ArgSer Gly

1385 1390 1395

Thr Asp Ala Glu Gly Val Pro Glu Val Ile Ala Glu Leu Thr Glu

1400 1405 1410

Gln Gly Ala Thr Val Ser Ala Val Ala Cys Asp Val Ser Asp Arg

1415 1420 1425

Asp Gln Val Lys Ala Leu Leu Ala Ala Val Pro Gln Glu His Arg

1430 1435 1440

Leu Thr Gly Val Val His Thr Ala Gly Val Phe Asp Asp Gly Leu

1445 1450 1455

Ile Ala Ala Leu Ser Pro Glu Arg Leu Glu Arg Val Phe Ala Pro

1460 1465 1470

Lys Val Asp Ala Val Arg His Leu Asp Glu Leu Thr Arg Asp Leu

1475 1480 1485

Asp Leu Asp Ala Phe Ile Val Phe Ser Ser Val Ala Gly Leu Ser

1490 1495 1500

Gly Gly Ala Gly Gln Gly Ser Tyr Ala Ala Ala Asn Ala Phe Leu

1505 1510 1515

Asp Gly Leu Met Ala Ser Arg Arg Ala Ala Gly Leu Pro Gly Leu

1520 1525 1530

Ser Leu Ala Trp Gly Leu Trp Glu Glu Ser Leu Gly Met Ala Ala

1535 1540 1545

Arg Leu Ser Ser Val Asp Arg Ala Arg Ala Ser Arg Gly Gly Val

1550 1555 1560

Arg Ala Ile Ala Ala Ala Glu Gly Met Ala Leu Phe Asp Ala Ala

1565 1570 1575

Ala Gly Ser Gly Gln Ser Leu Leu Val Pro Ile Lys Leu Asp Leu

1580 1585 1590

Arg Gly Leu Arg Ala Asp Thr Ala Ser Gly Gly Gly Val Pro Pro

1595 1600 1605

Leu Leu Arg Gly Leu Val Arg Ala Gly Arg Gln Pro Ala Arg Ala

1610 1615 1620

Ala Asp Thr Gly Asp Gly His Arg Arg Leu Ser Ala Arg Val Ala

1625 1630 1635

Gly Leu Ala Ala Ala Glu Gln Glu Ala Phe Leu Leu Asp Leu Val

1640 1645 1650

Arg Ala Glu Ala Ala Val Val Leu Gly His Thr Gly Pro Gly Gly

1655 1660 1665

Val Arg Glu Glu Val Ala Phe Arg Asp Ala Gly Phe Asp Ser Leu

1670 1675 1680

Thr Ala Val Glu Leu Arg Asn Arg Leu Arg Glu Ala Thr Gly Leu

1685 1690 1695

Lys Leu Pro Ala Thr Leu Val Phe Asp Tyr Pro Thr Pro Leu Ala

1700 1705 1710

Leu Ala Arg Arg Leu Arg Asp Gly Leu Gly Asp Ala Ser Pro Val

1715 1720 1725

Ser Asp Arg Pro Thr Ala Val Val Ala Asp Thr Gly Glu Pro Ile

1730 1735 1740

Ala Ile Val Ser Met Ala Cys Arg Tyr Pro Gly Gly Val Ser Ser

1745 1750 1755

Pro Glu Glu Leu Trp Arg Leu Val Ala Asp Gly Val Asp Ala Val

1760 1765 1770

Ser Asp Phe Pro Asp Asp Arg Gly Trp Asp Leu Glu Gly Leu Phe

1775 1780 1785

Asp Pro Asp Pro Asp His Ala Gly Thr Ser Tyr Thr Arg Gln Gly

1790 1795 1800

Gly Phe Leu Arg Gly Ala Gly Leu Phe Asp Ala Gly Phe Phe Gly

1805 1810 1815

Ile Ser Pro Arg Glu Ala Met Ala Met Asp Pro Gln Gln Arg Leu

1820 1825 1830

Leu Leu Glu Thr Ser Trp Glu Ala Leu Glu Ala Ala Gly Met Asp

1835 1840 1845

Pro Gly Ser Leu Lys Gly Thr Asp Val Gly Val Phe Thr Gly Val

1850 1855 1860

Ala Ser Gln GlyTyr Gly Thr Gly Val Val Ala Pro Glu Val Glu

1865 1870 1875

Gly Phe Ala Gly Thr Gly Leu Ala Gly Ser Val Ala Ser Gly Arg

1880 1885 1890

Val Ser Tyr Val Phe Gly Phe Glu Gly Pro Ala Val Ser Val Asp

1895 1900 1905

Thr Ala Cys Ser Ser Ser Leu Val Ala Met His Leu Ala Ala Gln

1910 1915 1920

Ala Leu Arg Gln Gly Glu Cys Ser Met Ala Leu Ala Gly Gly Ala

1925 1930 1935

Met Val Met Ala Thr Pro Gly Ala Phe Met Ala Phe Ser Arg Gln

1940 1945 1950

Arg Gly Leu Ala Asp Asp Gly Arg Cys Lys Ala Phe Ala Asp Gly

1955 1960 1965

Ala Asp Gly Met Gly Leu Ala Glu Gly Val Gly Val Val Leu Leu

1970 1975 1980

Glu Arg Leu Ser Ala Ala Arg Glu His Gly His Gln Val Leu Ala

1985 1990 1995

Val Leu Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn Gly

2000 2005 2010

Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val Ile Arg Lys

2015 20202025

Ala Leu Asp Thr Ala Arg Leu Thr Pro Ala Asp Val Asp Val Val

2030 2035 2040

Glu Gly His Gly Thr Gly Thr Ala Leu Gly Asp Pro Ile Glu Ala

2045 2050 2055

Gln Ala Leu Leu Ala Thr Tyr Gly Gln Gly Arg Asp Pro Gln Gln

2060 2065 2070

Pro Leu Trp Leu Gly Ser Leu Lys Ser Asn Ile Gly His Thr Gln

2075 2080 2085

Ala Ala Ala Gly Val Ala Ser Val Ile Lys Met Val Gln Ala Leu

2090 2095 2100

Arg His Gly Val Met Pro Pro Thr Leu His Val Arg Glu Pro Ser

2105 2110 2115

Asn Gln Val Asp Trp Ser Glu Gly Ala Val Glu Leu Leu Thr Glu

2120 2125 2130

Ala Lys Asp Trp Pro Arg Asn Gly Arg Pro Arg Arg Ala Gly Val

2135 2140 2145

Ser Ser Phe Gly Val Ser Gly Thr Asn Ala His Leu Ile Leu Glu

2150 2155 2160

Glu Ala Pro Glu Ala Pro Val Thr Glu Glu Ala Ala Ser Val Gly

2165 2170 2175

Ala Val Pro Leu Val Val Ser Ala Arg Ser Gln Gly Ser Leu Ala

2180 2185 2190

Gly Gln Ala Gly Arg Leu Ala Ser Tyr Val Glu Ser Asp Ala Gly

2195 2200 2205

Gly Ala Ser Leu Ala Asp Ile Ala Gly Ala Leu Val Ser Gly Arg

2210 2215 2220

Gly Val Phe Gly Glu Arg Ala Val Val Val Ala Gly Ser Glu Asp

2225 2230 2235

Glu Ala Leu Ser Gly Leu Arg Ala Leu Ala Arg Gly Ala Ser Asp

2240 2245 2250

Leu Asn Leu Val Ser Ala Ser Gly Gly Val Pro Gly Asn Val Val

2255 2260 2265

Trp Val Phe Pro Gly Gln Gly Ser Gln Trp Val Gly Met Gly Arg

2270 2275 2280

Glu Leu Leu Asp Thr Ser Pro Val Phe Ala Glu Arg Val Gly Glu

2285 2290 2295

Cys Ala Ala Thr Leu Glu Pro Leu Val Asp Trp Ser Leu Val Asp

2300 2305 2310

Val Leu Arg Gly Glu Ala Glu Pro Ala Leu Leu Glu Arg Val Asp

2315 2320 2325

Val Val Gln Pro Ala Ser Phe Ala Val Met Val Gly Leu Ala Ala

2330 2335 2340

Val Trp Ala Ser Ala Gly Val Ile Pro Asp Ala Val Val Gly His

2345 2350 2355

Ser Gln Gly Glu Ile Ala Ala Ala Cys Val Ala Gly Ala Leu Ser

2360 2365 2370

Leu Glu Asp Ala Val Arg Val Val Ala Leu Arg Ser Gln Ala Ile

2375 2380 2385

Ala Ala Arg Leu Ala Gly Arg Gly Gly Met Ala Ser Val Ala Leu

2390 2395 2400

Ser Glu Glu Glu Ala Ala Gly Arg Leu Glu Pro Trp Ala Glu Arg

2405 2410 2415

Val Glu Val Ala Ala Val Asn Gly Pro Ser Ser Val Val Val Ala

2420 2425 2430

Gly Asp Ala Gln Ala Leu Asp Glu Val Leu Glu Ala Leu Ser Ser

2435 2440 2445

Asp Gly Val Arg Thr Arg Arg Val Ala Val Asp Tyr Ala Ser His

2450 2455 2460

Thr Arg His Val Glu Asp Ile Arg Asp Thr Leu Ala Glu Ala Leu

2465 2470 2475

Ala Gly Val Ser Ala Lys Ala Pro Val Val Pro Phe Tyr Ser Thr

2480 2485 2490

Val Thr Gly Ser Trp Val Glu Asp Ala Gly Val Leu Asp Gly Arg

2495 2500 2505

Tyr Trp Tyr Arg Asn Leu Arg Gly Gln Val Arg Phe Gly Pro Ala

2510 2515 2520

Val Ala Asp Leu Leu Ser Gln Gly His Gly Val Phe Val Glu Val

2525 2530 2535

Ser Ala His Pro Val Leu Val Gln Pro Ile Thr Glu Ile Val Asp

2540 2545 2550

Asp Ala Asp Gly Ala Ala Val Val Thr Gly Ser Leu Arg Arg Glu

2555 2560 2565

Glu Gly Gly Leu Arg Arg Leu Leu Met Ser Met Ala Glu Leu Phe

2570 2575 2580

Val Arg Gly Val Ala Val Asp Trp Ser Ala Ile Val Pro Thr Gly

2585 2590 2595

Thr Ala Ser Gly Arg Val Ala Leu Pro Thr Tyr Ala Phe Asp His

2600 2605 2610

Gln His Tyr Trp Leu Lys Pro Gly Ala Gln Ala Thr Asp Ala Ala

2615 2620 2625

Ser Leu Gly Leu Ala Gly Ala Glu His Pro Leu Leu Gly Ala Val

2630 2635 2640

Val Arg Leu Pro Gln Ser Asp Gly Leu Val Phe Thr Ser Arg Leu

2645 2650 2655

Ser Leu Arg Thr Gln Pro TrpLeu Ala Asp His Ala Ile Gly Gly

2660 2665 2670

Val Val Ile Leu Pro Gly Thr Gly Leu Val Glu Leu Ala Val Arg

2675 2680 2685

Ala Gly Asp Glu Ala Gly Cys Gly Val Leu Glu Glu Leu Val Ile

2690 2695 2700

Glu Ala Pro Leu Val Val Pro Glu Arg Gly Gly Val Arg Val Gln

2705 2710 2715

Val Ser Leu Gly Gly Pro Gly Glu Asn Gly Val Arg Thr Val Glu

2720 2725 2730

Val Tyr Ser Gln Arg Glu Asp Ala Gly Asp Glu Ala Asp Ala Trp

2735 2740 2745

Thr Arg His Ala Thr Gly Leu Leu Ser Ala Ala Ala Arg Pro Asp

2750 2755 2760

Gly Val Arg Ala Gly Thr Gly Thr Gly Phe Asp Phe Thr Ala Trp

2765 2770 2775

Pro Pro Pro Gly Ala Arg Pro Val Glu Leu Gly Asp Leu Tyr Gly

2780 2785 2790

Asp Leu Ala Glu His Gly Tyr Ala Tyr Gly Pro Ala Phe Gln Gly

2795 2800 2805

Val Arg Ala Val Trp Arg Arg Gly Asp Glu Val Phe Ala Glu Val

2810 28152820

Val Leu Pro Glu Glu Gln Arg Lys Glu Ala Gly Arg Tyr Gly Ile

2825 2830 2835

His Pro Ala Leu Leu Asp Ala Ala Leu Gln Ala Gly Thr Ile Gly

2840 2845 2850

Ala Ala Ala Gly Pro Ala Val Glu Gly Ser Gly Glu Pro Val Leu

2855 2860 2865

Ala Phe Ala Trp Asn Gly Leu Val Leu His Ala Gly Gly Ala Ser

2870 2875 2880

Ala Leu Arg Val Arg Val Ala Pro Ser Gly Pro Asp Ala Leu Ser

2885 2890 2895

Val Glu Ala Ala Asp Glu Ala Gly Gly Leu Val Val Thr Met Asp

2900 2905 2910

Ser Leu Val Ser Arg Pro Val Ser Ala Asp Gln Leu Gly Ala Ala

2915 2920 2925

Thr Ala Ala Gln Ala Leu Phe His Val Asp Trp Thr Glu Leu Pro

2930 2935 2940

Pro Ala Gln Gly Ala Gly Ser Ala Leu Ser Trp Met Ser Val Ile

2945 2950 2955

Ala Ala Asp Glu Val Ala Thr Leu Ala Glu Glu Val Ala Ala Gly

2960 2965 2970

Ala Ala Val Pro Met Ala Ala Val Leu Glu Ala Phe Gly Gly Val

2975 2980 2985

Gly Gly Glu Asp Ala Val Leu Ala Leu Thr Ser Gln Val Leu Glu

2990 2995 3000

Val Val Gln Gly Trp Leu Ala Gly Ala Gly Leu Glu Glu Ser Arg

3005 3010 3015

Leu Val Val Val Thr Arg Gly Ala Val Pro Ala Gly Glu Gly Gly

3020 3025 3030

Val Thr Asp Pro Ala Ala Ala Ala Val Trp Gly Leu Val Arg Ala

3035 3040 3045

Ala Gln Ser Glu His Pro Asp Arg Ile Val Leu Leu Asp Leu Asp

3050 3055 3060

Pro Ala Ser Asp Asp Glu Ala Gly Ser Val Leu Asp Thr Val Leu

3065 3070 3075

Gly Ala Val Leu Ala Thr Gly Glu Pro Gln Ile Ala Leu Arg Gly

3080 3085 3090

Thr Thr Leu Ser Val Pro Arg Leu Ala Arg Val Ala Asp Gln Val

3095 3100 3105

Pro Gln Ser Pro Val Val Phe Gly Ser Glu Gly Thr Val Leu Val

3110 3115 3120

Ser Gly Gly Gly Ser Leu Gly Ala Leu Val Ala Arg His Leu Val

3125 3130 3135

Val Arg His Gly Val Arg Gly Leu Val Leu Ala Ser Arg Arg Gly

3140 3145 3150

Val Asp Ala Glu Gly Val Pro Glu Leu Val Ala Glu Leu Thr Glu

3155 3160 3165

Leu Gly Ala Thr Val Ser Ala Val Ala Cys Asp Val Ser Asp Arg

3170 3175 3180

Asp Gln Val Lys Ala Leu Leu Ala Ala Val Pro Gln Glu Arg Arg

3185 3190 3195

Leu Thr Gly Val Val His Thr Ala Gly Val Phe Asp Ala Gly Val

3200 3205 3210

Ile Gly Ala Leu Ser Pro Glu Arg Leu Ala Gly Val Phe Ala Pro

3215 3220 3225

Lys Val Asp Ala Val Arg His Leu Asp Glu Leu Thr Arg Gly Leu

3230 3235 3240

Asp Leu Arg Ala Phe Val Val Tyr Ser Ser Ala Ser Ser Val Phe

3245 3250 3255

Met Gly Ala Gly Ser Gly Gly Tyr Ala Ala Ala Asn Ala Phe Leu

3260 3265 3270

Asp Gly Leu Met Ala Gln Arg Arg Ala Ala Gly Leu Pro Gly Leu

3275 3280 3285

Ser Leu Ala Trp Gly Pro Trp Glu Gln Thr Thr Gly Met Ala Asp

32903295 3300

Thr Ile Asp Asp Leu Thr Arg Ala Arg Met Ser Arg Arg Glu Gly

3305 3310 3315

Arg Gly Gly Val Leu Ala Leu Lys Ser Ser Glu Gly Leu Glu Leu

3320 3325 3330

Phe Asp Ala Ala Leu Ala Ser Gly Trp Ala Leu Leu Val Pro Val

3335 3340 3345

Lys Leu Asp Leu Arg Asp Ala Arg Ala Asp Ala Val Ala Gly Gly

3350 3355 3360

Gly Val Pro Pro Leu Leu Arg Gly Leu Val Arg Ala Ala Arg Gln

3365 3370 3375

Pro Ala Arg Ala Ala Gly Gly Thr Gly Gly Asp His Arg Gln Leu

3380 3385 3390

Ala Glu Arg Leu Ala Gly Leu Ala Ala Ala Glu Arg Arg Lys Phe

3395 3400 3405

Leu Leu Asp Leu Val Arg Ala Gln Ala Ala Val Val Leu Gly His

3410 3415 3420

Thr Gly Pro Gly Ser Val Arg Pro Asp Thr Ala Phe Asn Glu Ala

3425 3430 3435

Gly Phe Asp Ser Leu Thr Ala Val Glu Leu Arg Asn Arg Leu Arg

3440 3445 3450

Glu Ala Thr Gly Leu Lys Leu Pro Ala Thr Leu Val Phe Asp His

3455 3460 3465

Pro Thr Pro Val Ala Leu Ser Ala Tyr Leu Leu Gly Glu Ile Ala

3470 3475 3480

Val Asp Ser Ala Ser Pro Ala Asp Pro Val Leu Ala Gly Leu Ala

3485 3490 3495

Gly Leu Glu Ala Ala Ile Glu Ser Ala Ala Gly Asp Glu Glu Ala

3500 3505 3510

Arg Asp Arg Ile Thr Val Arg Leu Arg Glu Leu Leu Lys Ala Ala

3515 3520 3525

Glu Ala Ala Glu Ala Ala Glu Ala Ala Gly Gly Arg Asp Ala Ala

3530 3535 3540

Pro Asp Ala Ala Gly Thr Ser Glu Asp Asp Leu Glu Asn Ala Ser

3545 3550 3555

Asp Glu Glu Leu Phe Ala Ile Phe Asp Arg Leu Asp

3560 3565 3570

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Val Ala Asp Glu Val Gln Leu Arg Asp Tyr Leu Lys Arg Ala Ile Ala

1 5 10 15

Asp Ala Arg Asp Ala Arg Lys Arg LeuArg Glu Val Glu Asp Gln Ala

20 25 30

Arg Glu Pro Ile Ala Ile Val Ser Met Ala Cys Arg Tyr Pro Gly Gly

35 40 45

Val Ser Ser Pro Glu Glu Leu Trp Lys Leu Val Ala Asp Gly Val Asp

50 55 60

Ala Val Ser Asp Phe Pro Asp Asp Arg Gly Trp Asp Leu Glu Gly Leu

65 70 75 80

Phe Asp Pro Asp Pro Asp His Ala Gly Thr Ser Tyr Thr Arg Gln Gly

85 90 95

Gly Phe Leu Arg Gly Ala Gly Leu Phe Asp Ala Gly Phe Phe Gly Ile

100 105 110

Ser Pro Arg Glu Ala Met Ala Met Asp Pro Gln Gln Arg Leu Leu Leu

115 120 125

Glu Thr Ser Trp Glu Ala Leu Glu His Ala Gly Ile Asp Pro Leu Ser

130 135 140

Leu Lys Gly Thr Asp Val Gly Val Phe Ser Gly Val Ser Ser Gln Gly

145 150 155 160

Tyr Gly Ala Gly Ala Gly Val Val Thr Pro Glu Ser Glu Gly Phe Ala

165 170 175

Gly Thr Gly Ala Thr Pro Ser Val Ala Ser Gly Arg Val Ser Tyr Val

180 185 190

Phe Gly Phe Glu Gly Pro Ala Val Thr Ile Asp Thr Ala Cys Ser Ser

195 200 205

Ser Leu Val Ala Ile His Leu Ala Ala Gln Ala Leu Arg Asn Gly Asp

210 215 220

Cys Ser Met Ala Leu Ala Gly Gly Ala Met Val Met Ala Thr Pro Gly

225 230 235 240

Asn Phe Val Ala Phe Ser Arg Gln Arg Gly Leu Ala Thr Asp Gly Arg

245 250 255

Cys Lys Ala Phe Ala Asp Gly Ala Asp Gly Met Gly Leu Ala Glu Gly

260 265 270

Ala Gly Val Val Leu Leu Glu Arg Leu Ser Val Ala Arg Glu Arg Gly

275 280 285

His Gln Val Leu Ala Val Leu Arg Gly Ser Ala Val Asn Gln Asp Gly

290 295 300

Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Gln Arg Val

305 310 315 320

Ile Arg Arg Ala Leu Ala Asn Ala Asp Leu Ser Pro Ser Asp Val Asp

325 330 335

Val Val Glu Gly His Gly Thr Gly Thr Ser Leu Gly Asp Pro Ile Glu

340 345 350

Ala Gln Ala Leu Leu Ala Thr Tyr Gly Lys Gly Arg Asp Pro Gln Gln

355 360 365

Pro Leu Trp Leu Gly Ser Leu Lys Ser Asn Ile Gly His Thr Gln Ala

370 375 380

Ala Ala Gly Val Gly Ser Val Ile Lys Met Val Gln Ala Leu Arg His

385 390 395 400

Gly Val Met Pro Pro Thr Leu His Val Gln Glu Pro Thr Thr Glu Val

405 410 415

Asp Trp Ser Ala Gly Ala Val Glu Leu Leu Thr Glu Ala Arg Ala Trp

420 425 430

Pro Arg Asp Ala Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Val

435 440 445

Ser Gly Thr Asn Ala His Leu Ile Leu Glu Glu Val Pro Glu Glu Pro

450 455 460

Val Ser Glu Glu Pro Val Pro Glu Glu Ser Gly Ser Ser Gly Val Val

465 470 475 480

Pro Leu Val Val Ser Ala Arg Gly His Ala Ala Leu Ser Gly Gln Ala

485 490 495

Ala Arg Leu Ala Ala Arg Val Glu Thr Val Gly Glu Val Ser Leu Ala

500 505 510

Gly Val Ala Gly Ala Leu Val Ser Gly Arg Ala Leu Leu Asp Asp Arg

515 520 525

Ala Val Val Val Ala Gly Ser Ala Glu Asp Ala Leu Ala Gly Leu Gly

530 535 540

Ala Leu Ala Arg Gly Glu Ser Gly Pro Asp Val Val Ser Gly Ser Val

545 550 555 560

Ala Gly Ser Gly Glu Pro Gly Gly Leu Val Leu Val Phe Pro Gly Gln

565 570 575

Gly Ser Gln Trp Ala Gly Met Gly Arg Glu Leu Leu Asp Ser Ser Pro

580 585 590

Val Phe Ala Glu Arg Val Ala Glu Cys Ala Ala Ala Leu Glu Pro Trp

595 600 605

Val Asp Trp Ser Leu Val Asp Val Leu Arg Gly Glu Val Glu Pro Ala

610 615 620

Leu Leu Glu Arg Val Asp Val Val Gln Pro Ala Ser Phe Ala Val Met

625 630 635 640

Val Gly Leu Ala Ser Val Trp Ala Ser Val Gly Val Lys Pro Asp Ala

645 650 655

Val Leu Gly His Ser Gln Gly Glu Ile Ala Ala Ala Cys Val Ala Gly

660 665 670

Val Leu Ser Leu Glu Asp Ala Val Arg Val Val Ala Leu Arg Ser Gln

675 680 685

Ala Ile Ala Ala Arg Leu Ala Gly Arg Gly Gly Met Ala Ser Val Ala

690 695 700

Leu Gly Pro Gln Glu Val Ala Gly Arg Leu Glu Pro Trp Ala Glu Arg

705 710 715 720

Val Glu Val Ala Ala Val Asn Gly Pro Ser Ser Val Val Ile Ala Gly

725 730 735

Asp Ala Glu Ala Leu Asp Glu Ala Leu Arg Glu Leu Ser Gly Asp Gly

740 745 750

Val Arg Val Arg Arg Val Ala Val Asp Tyr Ala Ser His Thr Arg His

755 760 765

Val Glu Asp Ile Arg Asp Thr Leu Ala Glu Ala Leu Thr Gly Val Ser

770 775 780

Ala Gln Ala Pro Ala Val Pro Phe Tyr Ser Thr Val Ser Gly Gly Trp

785 790 795 800

Val Gln Glu Ala Gly Val Leu Asp Ser Glu Tyr Trp Tyr Arg Asn Leu

805 810 815

Arg Gly Gln Val Gly Phe Gly Pro Ala Val Ala Asp Leu Leu Ala Gln

820 825 830

Gly His Gly Val Phe Val Glu Val Ser Ala His Pro Val Leu Val Gln

835 840 845

Pro Val Ser Glu Ile Val Asp Glu Ser Gly Ala Asp Ala Val Val Thr

850 855 860

Gly Ser Leu Arg Arg Asp Glu Gly Gly Leu Arg Arg Leu Leu Met Ser

865 870 875 880

Met Ala Glu Leu Phe Val Arg Gly Val Ala Val Asp Trp Ser Ala Ile

885 890 895

Val Pro Thr Gly Thr Ala Ser Gly Arg Val Ala Leu Pro Thr Tyr Ala

900 905 910

Phe Asp His Gln His Tyr Trp Leu Lys Pro Gly Glu Ser Ala Ser Asp

915 920 925

Ala Ala Ser Leu Gly Gln Thr Pro Ala Glu His Pro Leu Leu Gly Ala

930 935 940

Val Val Arg Leu Pro His Ser Asp Ser Leu Val Phe Thr Ser Arg Leu

945 950 955 960

Ser Leu Arg Ser His Pro Trp Leu Ala Asp His Ala Val Gly Gly Val

965 970 975

Val Ile Leu Pro Gly Thr Gly Leu Val Glu Leu Ala Val Arg Ala Gly

980 985 990

Asp Glu Ala Gly Cys Gly Val Leu Glu Glu Leu Val Ile Glu Ala Pro

995 1000 1005

Leu Val Val Ala Glu His Gly Gly Val Arg Met Gln Ile Ala Val

1010 1015 1020

Gly Ala Pro Gly Glu Ser Gly Ala Arg Thr Val Glu Val Tyr Ser

1025 1030 1035

Gln Arg Ala Asp Ala Gly Pro Ala Asp Ala Trp Thr Arg His Ala

1040 1045 1050

Thr Gly Leu Leu Thr Ala Ser Gln Ala Thr Pro Ala Glu Val Arg

1055 1060 1065

Glu Tyr Asp Phe Ala Ala Trp Pro Pro Pro Gly Ala Arg Lys Val

1070 1075 1080

Glu Val Glu Ala Ser Asp Phe Tyr Asp Gly Leu Val Ala Arg Gly

1085 1090 1095

Phe Gly Tyr Gly Pro Ala Phe Gln Gly Val Arg Ala Ala Trp Arg

1100 1105 1110

Arg Gly Asp Glu Val Phe Ala Glu Val Ala Leu Pro Glu Glu Gln

1115 1120 1125

Arg Lys Glu Ala Gly Arg Tyr Gly Ile His Pro Ala Leu Leu Asp

1130 1135 1140

Ala Ala Leu Gln Ala Gly Thr Phe Ala Ala Glu Ala Asp Pro Thr

1145 1150 1155

Ala Glu Asp Ser Gly Glu Pro Ile Leu Ala Phe Ala Trp Asn Gly

1160 1165 1170

Leu Val Leu His Ala Ala Gly Ala Ser Ala Leu Arg Val Arg Ile

1175 1180 1185

Ala Pro Cys Gly Pro Asp Ala Leu Ala Val Gln Ala Ala Asp Glu

1190 1195 1200

Thr Gly Gly Leu Val Val Thr Met Asp Ser Leu Val Ser Arg Pro

1205 1210 1215

Val Ser Ala Asp Gln Leu Gly Ala Ala Thr Asp Gly Ala Val Ala

1220 1225 1230

Asp Ser Leu Tyr His Val Asp Trp Thr Glu Leu Pro Pro Ala Gln

1235 1240 1245

Val Arg Glu Pro Ala Pro Pro Trp Ala Ala Val Ala Thr Ala Ala

1250 1255 1260

Glu Val Ala Ala Leu Ala Gly Ser Pro Thr Val Pro Pro Val Ala

1265 1270 1275

Val Leu Glu Ala Val Gly Asp Asp Gly Ala Asp Ala Val Leu Ala

1280 1285 1290

Leu Thr Ser Arg Val Leu Ala Val Val Gln Ala Trp Leu Ala Glu

12951300 1305

Ala Gly Leu Glu Glu Ser Arg Leu Val Val Val Thr Arg Gly Ala

1310 1315 1320

Val Ser Ala Gly Glu Asp Ala Val Leu Asp Pro Ala Gly Ala Ala

1325 1330 1335

Val Trp Gly Leu Val Arg Ala Ala Gln Ala Glu Asn Pro Asp Arg

1340 1345 1350

Ile Val Leu Leu Asp Thr Asp Pro Val Ser Gln Glu Gly Leu Ala

1355 1360 1365

Ser Leu Leu Gly Pro Ala Leu Ala Thr Gly Glu Pro Gln Val Ala

1370 1375 1380

Val Arg Gly Ala Thr Leu Ser Val Pro Arg Leu Asp Arg Val Thr

1385 1390 1395

Asp Gln Val Pro Gly Asn Pro Val Val Phe Gly Ser Glu Gly Thr

1400 1405 1410

Val Leu Val Ser Gly Gly Gly Ser Leu Gly Ala Leu Val Ala Arg

1415 1420 1425

His Leu Val Val Arg His Gly Val Arg Gly Leu Val Leu Ala Ser

1430 1435 1440

Arg Arg Gly Val Asp Ala Glu Gly Val Pro Glu Leu Val Ala Glu

1445 1450 1455

Leu Thr Glu Leu Gly Ala Thr Val Ser Ala Val Ala Cys Asp Val

1460 1465 1470

Ser Asp Arg Asp Gln Val Lys Ala Leu Leu Ala Ala Val Ala Asp

1475 1480 1485

Asp His Glu Arg Arg Leu Thr Gly Val Val His Thr Ala Gly Val

1490 1495 1500

Phe Asp Ala Gly Val Ile Gly Ala Leu Ser Pro Glu Arg Leu Ala

1505 1510 1515

Gly Val Phe Ala Pro Lys Val Asp Ala Val Arg His Leu Asp Glu

1520 1525 1530

Leu Thr Arg Gly Leu Gly Leu Cys Ala Phe Val Val Tyr Ser Ser

1535 1540 1545

Ala Ser Ser Val Phe Met Gly Ala Gly Ser Gly Gly Tyr Ala Ala

1550 1555 1560

Ala Asn Ala Phe Leu Asp Gly Leu Met Ala Gln Arg Arg Ala Ala

1565 1570 1575

Gly Leu Pro Gly Leu Ser Leu Ala Trp Gly Thr Trp Glu His Ala

1580 1585 1590

Thr Asn Met Thr Thr His Leu Ser Thr Ala Asp Arg Thr Arg Met

1595 1600 1605

Ser Arg Arg Ala Asn Arg Thr Gly Val Val Gly Ile Lys Pro Ala

1610 1615 1620

Glu Gly Met Glu Leu Phe Asp Ala Ala Val Ala Ser Gly Arg Ala

1625 1630 1635

Leu Leu Val Pro Val Lys Leu Asp Leu Gln Gly Val Arg Ala Gly

1640 1645 1650

Ala Ala Gly Gly Gly Gly Val Pro Pro Leu Leu Arg Gly Leu Val

1655 1660 1665

Pro Ala Ala Arg Gln Gln Ala Arg Ala Ala Ala Ala Asn Glu Gly

1670 1675 1680

Asp Leu Val Arg Arg Leu Ala Gln Leu Thr Gly Pro Glu Gln Glu

1685 1690 1695

Ala Leu Leu Leu Asp Leu Val Arg Thr Gln Ala Ala Val Val Leu

1700 1705 1710

Gly His Ser Gly Pro Gly Gly Val Arg Ala Asp Thr Ala Phe Lys

1715 1720 1725

Asp Val Gly Phe Asp Ser Leu Thr Ser Val Glu Leu Arg Asn Arg

1730 1735 1740

Leu Arg Glu Ala Thr Gly Leu Lys Leu Pro Ala Thr Leu Val Phe

1745 1750 1755

Asp Gln Pro Asn Pro Leu Val Leu Ala Arg His Leu Arg Asp Gly

1760 1765 1770

Leu Gly Val Ser Asp Asp Val Leu Ala Arg Val His Ala Arg Ile

1775 1780 1785

Glu Asp Val Glu Thr Leu Ile Gly Gly Leu Ser Leu Asp Ala Ala

1790 1795 1800

Met Lys Ser Ser Val Ala Leu Arg Leu Gln Gly Leu Val Ala Arg

1805 1810 1815

Cys Asn Gly Val Leu Glu Gln Thr Asp Val Ser Thr Val Ala Asp

1820 1825 1830

Lys Leu Glu Ser Ala Ser Ala Asp Glu Val Leu Asp Phe Ile Asp

1835 1840 1845

Asp Glu Leu Gly Leu Val

1850

<210>14

<211>3570

<212>PRT

<213>Streptomyces spectabilis

<400>14

Met Ala Thr Asp Asp Lys Leu Leu Lys Tyr Leu Lys Arg Val Thr Thr

1 5 10 15

Glu Leu His Gln Leu Arg Lys Gln Gly Ser Ala His Pro Gly Glu Pro

20 25 30

Val Ala Ile Val Gly Met Ala Cys Arg Leu Pro Gly Gly Val Thr Gly

35 40 45

Pro Glu Asp Leu Trp Arg Leu Val Arg Glu Gly Arg Asp Ala Val Ser

5055 60

Gly Phe Pro Asp Asp Arg Gly Trp Glu Leu Glu Gly Leu Phe Asp Pro

65 70 75 80

Asp Pro Asp Asn Pro Gly Thr Ser Tyr Thr Asp Gln Gly Gly Phe Leu

85 90 95

Arg Gly Ala Gly Leu Phe Asp Ala Gly Phe Phe Gly Ile Ser Pro Arg

100 105 110

Glu Ala Leu Ala Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser

115 120 125

Trp Glu Ala Leu Glu His Ala Gly Val Asp Pro Leu Ser Leu Lys Gly

130 135 140

Thr Asp Val Gly Val Phe Ser Gly Val Ser Ser Gln Gly Tyr Gly Ala

145 150 155 160

Gly Ala Gly Val Val Ala Pro Glu Leu Glu Ser Phe Thr Gly Thr Gly

165 170 175

Val Ala Gly Ser Val Ala Ser Gly Arg Val Ser Tyr Val Phe Gly Phe

180 185 190

Glu Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val

195 200 205

Ala Ile His Leu Ala Ala Gln Ala Leu Arg Gln Gly Glu Cys Ser Met

210 215220

Ala Leu Ala Gly Gly Ala Met Val Met Ser Thr Pro Gly Thr Phe Val

225 230 235 240

Ala Phe Ser Arg Gln Arg Gly Met Ala Ser Asp Gly Arg Cys Lys Ala

245 250 255

Tyr Ala Asp Gly Ala Asp Gly Met Gly Leu Ala Glu Gly Ala Gly Val

260 265 270

Val Val Leu Glu Arg Leu Ser Val Ala Gln Glu Arg Gly His Arg Val

275 280 285

Leu Ala Val Val Arg Gly Ser Ala Val Asn Gln Asp Gly Ala Ser Asn

290 295 300

Gly Leu Thr Ala Pro Asn Gly Pro Ser Gln Glu Arg Val Ile Arg Lys

305 310 315 320

Ala Leu Ala Gly Ala Gly Leu Val Ser Ala Asp Val Asp Val Val Glu

325 330 335

Gly His Gly Thr Gly Thr Ala Leu Gly Asp Pro Ile Glu Ala Gln Ala

340 345 350

Leu Leu Ala Thr Tyr Gly Gln Gly Arg Ser Pro Arg Gln Pro Ile Trp

355 360 365

Leu Gly Ser Leu Lys Ser Asn Ile Gly His Thr Gln Ala Ala Ala Gly

370 375380

Val Ala Ser Val Ile Lys Met Val Gln Ala Leu Arg His Gly Val Met

385 390 395 400

Pro Pro Thr Leu His Val Gln Glu Pro Thr Thr Glu Val Asp Trp Ser

405 410 415

Ala Gly Ala Val Glu Leu Leu Thr Glu Ala Arg Ala Trp Pro Arg Ala

420 425 430

Thr Arg Pro Arg Arg Ala Gly Ile Ser Ser Phe Gly Ile Ser Gly Thr

435 440 445

Asn Ala His Leu Ile Leu Glu Glu Ala Pro Ala Asp Glu Ala Glu Pro

450 455 460

Ala Pro Ala Glu Ala Glu Val Glu Ala Glu Pro Gly Gly Val Val Pro

465 470 475 480

Leu Val Val Ser Ala Ser Ser Gly Gly Gly Leu Thr Gly Gln Ala Ala

485 490 495

Arg Leu Thr Ala Tyr Leu Glu Ala Pro Gly Thr Ser Leu Ala Gly Val

500 505 510

Ala Gly Ala Leu Leu Ser Asp Arg Ala Leu Leu Asp Glu Arg Ala Val

515 520 525

Val Leu Ala Arg Thr Asp Thr Glu Ala Leu Ala Gly Leu Gly Ala Leu

530 535 540

Ala Arg Gly Glu Ser Ala Thr Gly Val Val Ser Gly Arg Ile Ala Gly

545 550 555 560

Ser Gly Val Pro Gly Lys Val Val Trp Val Phe Pro Gly Gln Gly Ser

565 570 575

Gln Arg Val Gly Ala Gly Arg Glu Leu Tyr Asp Arg His Pro Leu Phe

580 585 590

Ala Ala Ala Phe Asp Glu Ala Cys Glu His Leu Asp Ala Arg Leu Ala

595 600 605

Gly Trp Val Asp His Pro Val Arg Asp Val Val Leu Gly Thr Val Ala

610 615 620

Gly Ser Ala Asp Leu Leu Asn Gln Thr Val Phe Thr Gln Ala Gly Leu

625 630 635 640

Phe Ala Val Glu Ser Ala Leu Ala Arg Leu Val Glu Ser Trp Gly Val

645 650 655

Arg Pro Asp Val Val Ile Gly His Ser Ile Gly Glu Val Thr Ala Ala

660 665 670

Tyr Val Ala Gly Ala Leu Thr Leu Pro Asp Ala Ala Arg Leu Val Ala

675 680 685

Ala Arg Gly Arg Leu Met Gln Ala Leu Pro Pro Gly Gly Ala Met Val

690 695 700

Ala Val Ala Ala Thr Glu Glu Gln Val Thr Gly Leu Leu Gly Ala Gly

705 710 715 720

Val Glu Ile Ala Ala Val Asn Gly Pro Ser Ala Leu Val Leu Ser Gly

725 730 735

Asp Glu Asp Ala Val Leu Ala Ala Ala Asp Gln Leu Arg Glu Arg Gly

740 745 750

His Lys Ala Lys Arg Leu Ala Val Ser His Ala Phe His Ser Ala Arg

755 760 765

Met Glu Pro Met Leu Ala Asp Phe Thr Ala Glu Leu Ala Thr Val Ala

770 775 780

Trp Arg Gln Pro Ala Ile Pro Val Ile Ser Asn Val Thr Gly Arg Pro

785 790 795 800

Ala Glu Pro Asp Ala Leu Ala Asp Pro Arg Tyr Trp Ala Glu His Val

805 810 815

Arg Arg Pro Val Arg Phe Ala Glu Gly Ile Ala Ala Ala Val Glu Cys

820 825 830

Gly Gly Thr Leu Phe Val Glu Leu Gly Pro Gly Ala Ala Leu Thr Gly

835 840 845

Leu Val Glu Glu Thr Ala Thr Ala Ser Thr Ala Ala Val Thr Cys Val

850 855 860

Ala Ala Leu Arg Glu Gly Arg Pro Glu Glu Arg Thr Leu Leu Ala Ser

865 870 875 880

Thr Ala Glu Leu Phe Val Arg Gly Val Thr Val Asp Trp Thr Arg Leu

885 890 895

Leu Pro Ala Gly Ala Ala Ser Ala Pro Val Asp Leu Pro Thr Tyr Ala

900 905 910

Phe Asp His Arg His Tyr Trp Leu Gln Pro Gly Ala Ser Ala Thr Asp

915 920 925

Ala Val Ser Leu Gly Gln Thr Pro Ala Asp His Pro Leu Leu Gly Ala

930 935 940

Val Val Arg Leu Pro His Ser Asp Gly Leu Val Phe Thr Ser Arg Leu

945 950 955 960

Ser Leu Arg Ser His Pro Trp Leu Ala Asp His Ala Val Gly Gly Ala

965 970 975

Val Leu Val Pro Gly Thr Ala Leu Val Glu Leu Ala Val Arg Ala Gly

980 985 990

Asp Glu Ala Gly Cys Ala Ala Leu Glu Glu Leu Val Val Glu Ala Pro

995 1000 1005

Leu Val Val Pro Ala Arg Gly Ala Val Arg Val Gln Val Ala Val

1010 1015 1020

Gly Ala Pro Gly Ala Thr Gly Ala Lys Gly Ser Arg Thr Val Glu

1025 1030 1035

Val Tyr Ser Gln Pro Glu Asp Ala Ala Asp Gly Gly Gly Thr Asp

1040 1045 1050

Thr Trp Thr Arg His Ala Ala Gly Leu Leu Ser Val Thr Val Pro

1055 1060 1065

Pro Arg Gly Asp Ser Ala Phe Asp Phe Thr Ala Trp Pro Pro Arg

1070 1075 1080

Gly Ala Gln Pro Ile Asp Val His Pro Asp Asp Phe Tyr Asp Gly

1085 1090 1095

Leu Thr Glu Arg Gly Tyr Gly Tyr Gly Pro Ala Phe Arg Gly Leu

1100 1105 1110

Arg Ala Val Trp Arg Arg Gly Glu Glu Leu Phe Ala Glu Val Ala

1115 1120 1125

Leu Pro Asp Glu Gln Arg Lys Asp Ala Glu Arg Phe Gly Ile His

1130 1135 1140

Pro Ala Leu Leu Asp Ala Ala Leu His Ala Gly Met Leu Ala Thr

1145 1150 1155

Thr Ala Asp Thr Thr Ala Asp Asp Pro Gly Gln Pro Val Leu Pro

1160 1165 1170

Phe Ala Trp Asn Gly Leu Val Leu His Ala Ala Gly Ala Ser Thr

1175 1180 1185

Leu Arg Val Arg Leu Thr Pro Gly Gly Pro Gly Ala Val Ser Leu

1190 1195 1200

Thr Ala Ala Asp Glu Thr Gly Gly Leu Val Val Ala Met Asp Ser

1205 1210 1215

Leu Val Ser Arg Pro Val Ser Ala Gly Gln Leu Glu Ser Ala Thr

1220 1225 1230

Asp Thr Ala Ala Ala Asp Ala Leu Phe Ala Val Glu Trp Thr Glu

1235 1240 1245

Leu Pro Ser Val Arg Gly Asp Ala Pro Ser Trp Val Pro Val Ala

1250 1255 1260

Thr Ala Asp Asp Val Met Ala Leu Asp Arg Ser Ala Thr Ala Pro

1265 1270 1275

Pro Leu Ala Val Leu Glu Ala Phe Gly Gly Glu Gly Glu Gly Ala

1280 1285 1290

Ala Leu Ala Leu Thr Ser Gln Val Leu Glu Val Val Gln Ala Trp

1295 1300 1305

Leu Thr Gly Ala Gly Phe Gly Asp Ser Arg Leu Val Val Val Thr

1310 1315 1320

Arg Gly Ala Val Pro Ala Gly Asp Gly Ala Val Thr Asp Pro Ala

1325 1330 1335

Gly Ala Ala Val Trp Gly LeuVal Arg Ala Ala Gln Ala Glu Asn

1340 1345 1350

Pro Asp Arg Ile Leu Leu Ile Asp Thr Asp Pro Thr Ala Glu Gly

1355 1360 1365

Asp Leu Asp Ala Ala Leu Gly Val Ala Leu Ala Ser Gly Glu Pro

1370 1375 1380

Gln Val Ala Val Arg Gly Ala Thr Phe Ser Val Pro Arg Leu Val

1385 1390 1395

Arg Val Ala Gly Arg Gly Leu Gly Ser Gly Asn Val Phe Ala Ala

1400 1405 1410

Gly Lys Thr Val Leu Leu Ser Gly Gly Thr Gly Ser Leu Gly Gly

1415 1420 1425

Leu Val Ala Arg His Leu Val Thr Arg His Gly Val Arg His Leu

1430 1435 1440

Leu Leu Val Ser Arg Arg Gly Ala Asp Ala Glu Gly Val Pro Glu

1445 1450 1455

Leu Val Ala Glu Leu Ala Glu Gln Gly Ala Val Val Ser Val Val

1460 1465 1470

Ala Cys Asp Val Ser Asp Arg Asp Gln Val Arg Ala Leu Leu Ala

1475 1480 1485

Ala Val Pro Asp Glu His Pro Leu Ser Gly Val Val His Thr Ala

1490 14951500

Gly Val Leu Asp Asp Gly Val Ile Gly Ala Leu Thr Pro Glu Arg

1505 1510 1515

Leu Ala Gly Val Phe Ala Pro Lys Val Asp Ala Val Arg His Leu

1520 1525 1530

Asp Glu Leu Thr Arg Glu Leu Gly Leu Glu Leu Asp Ala Phe Ile

1535 1540 1545

Val Tyr Ser Ser Ala Ser Gly Val Phe Gly Ser Ala Gly Gln Gly

1550 1555 1560

Asn Tyr Ala Ala Ala Asn Ala Tyr Leu Asp Gly Leu Met Ala Asn

1565 1570 1575

Arg Arg Ala Ala Gly Leu Pro Gly Ile Ser Leu Ala Trp Gly Leu

1580 1585 1590

Trp Glu Gln Thr Ala Gly Met Thr Ala His Leu Asp Glu Val Asp

1595 1600 1605

Gln Ala Arg Ala Ser Arg Gly Gly Val Leu Ala Ile Thr Pro Ala

1610 1615 1620

Glu Gly Met Glu Leu Phe Asp Ala Ala Ala Ala Ser Arg His Ala

1625 1630 1635

Leu Leu Val Pro Ile Lys Leu Asp Leu Arg Gly Ala Arg Ala Gly

1640 1645 1650

Ala Ala Ser Gly Gly Ala Val Pro His Leu Leu Arg Gly Leu Val

1655 1660 1665

Arg Thr Gly Arg Gln Gln Ala Arg Ala Ala Ser Gly Ser Asp Gly

1670 1675 1680

Glu Leu Ala Arg Arg Leu Ala Gly Leu Thr Glu Ala Glu Gln Lys

1685 1690 1695

Ala Leu Leu Leu Asp Leu Val Arg Thr Gln Ala Ala Val Val Leu

1700 1705 1710

Gly His Ala Gly Pro Gly Ser Val Arg Pro Asp Met Ala Phe Asn

1715 1720 1725

Glu Ala Gly Phe Asp Ser Leu Thr Ser Val Glu Leu Arg Asn Arg

1730 1735 1740

Leu Arg Glu Ala Thr Ala Leu Lys Phe Pro Ala Thr Leu Val Phe

1745 1750 1755

Asp Tyr Pro Thr Pro Glu Ala Leu Ala Arg Tyr Leu Arg Glu Glu

1760 1765 1770

Leu Ala Asp Ser Ala Glu Ala Ala Ala Pro Ala Ala Ala Val Val

1775 1780 1785

Ala Asp Pro Asp Glu Pro Ile Ala Ile Val Gly Met Ala Cys Arg

1790 1795 1800

Leu Pro Gly Gly Val Thr Asp Pro Asp Gly Leu Trp Arg Leu Val

1805 1810 1815

Arg Glu Gly Arg Glu Gly Val Ser Pro Phe Pro Glu Asp Arg Gly

1820 1825 1830

Trp Asp Leu Glu Gly Leu Phe Asp Ala Asp Pro Asp Asn Ala Gly

1835 1840 1845

Thr Ser Tyr Ala Ser Gln Gly Gly Phe Leu His Gly Ala Gly Leu

1850 1855 1860

Phe Asp Pro Gly Phe Phe Gly Ile Ser Pro Arg Glu Ala Gln Ala

1865 1870 1875

Met Asp Pro Gln Gln Arg Leu Leu Leu Glu Thr Ser Trp Glu Ala

1880 1885 1890

Leu Glu Gln Ala Gly Ile Asp Pro Leu Ser Leu Lys Gly Thr Asp

1895 1900 1905

Val Gly Val Phe Ser Gly Val Ser Thr Gln Gly Tyr Gly Met Gly

1910 1915 1920

Ala Ala Ala Arg Asp Val Glu Gly Tyr Thr Gly Thr Gly Ser Ala

1925 1930 1935

Pro Ser Val Ala Ser Gly Arg Val Ser Tyr Ala Phe Gly Phe Glu

1940 1945 1950

Gly Pro Ala Val Thr Val Asp Thr Ala Cys Ser Ser Ser Leu Val

1955 1960 1965

Ala Met His Leu Ala Ala Gln Ala Leu Arg Gln Gly Glu Cys Ser

19701975 1980

Met Ala Leu Ala Gly Gly Val Ala Val Met Ala Thr Pro Gly Ala

1985 1990 1995

Phe Val Glu Phe Ser Arg Gln Arg Gly Leu Ala Thr Asp Gly Arg

2000 2005 2010

Cys Lys Ser Tyr Ala Ala Gly Ala Asp Gly Thr Gly Trp Ser Glu

2015 2020 2025

Gly Val Gly Leu Val Val Leu Glu Arg Leu Ser Ala Ala Arg Glu

2030 2035 2040

Arg Gly His Arg Val Leu Ala Val Leu Arg Gly Ser Ala Val Asn

2045 2050 2055

Gln Asp Gly Ala Ser Asn Gly Leu Thr Ala Pro Asn Gly Pro Ser

2060 2065 2070

Gln Gln Arg Val Ile Arg Arg Ala Leu Ala Asn Ala Gly Leu Ser

2075 2080 2085

Pro Ser Asp Val Asp Val Val Glu Gly His Gly Thr Gly Thr Thr

2090 2095 2100

Leu Gly Asp Pro Ile Glu Ala Gln Ala Leu Leu Ala Thr Tyr Gly

2105 2110 2115

Gln Gly Arg Asp Pro Glu Gln Pro Leu Trp Leu Gly Ser Leu Lys

2120 2125 2130

Ser Asn Ile Gly His Ala Gln Ala Ala Ala Gly Ala Ala Gly Val

2135 2140 2145

Ile Lys Met Val Gln Ala Leu Arg His Ala Phe Met Pro Pro Thr

2150 2155 2160

Leu His Val Asp Glu Pro Thr Ala Glu Val Asp Trp Ser Ala Gly

2165 2170 2175

Ala Val Glu Leu Leu Thr Glu Ala Arg Glu Trp Pro His Asn Gly

2180 2185 2190

Arg Pro Arg Arg Ala Gly Val Ser Ser Phe Gly Ile Ser Gly Thr

2195 2200 2205

Asn Ala His Leu Ile Leu Glu Glu Ser Pro Glu Glu Pro Val Ser

2210 2215 2220

Glu Glu Ser Gly Pro Val Gly Val Val Pro Leu Val Val Ser Ala

2225 2230 2235

Arg Ser Arg Ala Ser Leu Thr Gly Gln Ala Gly Arg Leu Val Ser

2240 2245 2250

Tyr Val Gly Ser Gly Ala Gly Gly Ser Ser Leu Val Gly Val Ala

2255 2260 2265

Gly Ala Leu Val Ser Gly Arg Ala Val Leu Gly Glu Arg Ala Val

2270 2275 2280

Val Val Ala Gly Ser Glu Gly Glu Ala Val Ser Gly Leu Arg Ala

2285 2290 2295

Leu Ala Arg Gly Glu Ser Asp Val Asn Leu Val Ser Gly Gly Gly

2300 2305 2310

Gly Val Pro Gly Lys Val Val Trp Val Phe Pro Gly Gln Gly Ser

2315 2320 2325

Gln Trp Ala Gly Met Gly Arg Glu Leu Leu Asp Ser Ser Pro Val

2330 2335 2340

Phe Ala Glu Arg Val Gly Glu Cys Ala Ala Ala Leu Gly Pro Leu

2345 2350 2355

Val Asp Trp Ser Leu Val Asp Val Leu Arg Gly Glu Val Glu Pro

2360 2365 2370

Ala Leu Leu Glu Arg Val Asp Val Val Gln Pro Ala Ser Phe Ala

2375 2380 2385

Val Met Val Gly Leu Ala Ser Val Trp Ala Ser Val Gly Val Lys

2390 2395 2400

Pro Asp Ala Val Leu Gly His Ser Gln Gly Glu Ile Ala Ala Ala

2405 2410 2415

Cys Val Ala Gly Ala Leu Ser Leu Glu Asp Ala Ala Arg Val Val

2420 2425 2430

Ala Leu Arg Ser Gln Ala Ile Ala Ala Arg Leu Ala Gly Arg Gly

2435 2440 2445

Gly Met Ala Ser Val Ala Leu Ala Glu Gln Glu Val Ala Gly Arg

2450 2455 2460

Leu Glu Pro Trp Ala Glu Arg Val Glu Val Ala Ala Val Asn Gly

2465 2470 2475

Pro Ser Ser Val Val Ile Ala Gly Asp Ala Glu Ala Leu Asp Glu

2480 2485 2490

Val Leu Gln Val Leu Ser Gly Asp Gly Val Arg Val Arg Arg Val

2495 2500 2505

Ala Val Asp Tyr Ala Ser His Thr Arg His Val Glu Asp Ile Glu

2510 2515 2520

Asp Thr Leu Ala Glu Ala Leu Ala Gly Val Ser Ala Gln Ala Pro

2525 2530 2535

Ala Val Pro Phe Tyr Ser Thr Val Thr Gly Ser Trp Val Glu Asp

2540 2545 2550

Ala Gly Val Leu Asp Gly Gly Tyr Trp Tyr Arg Asn Leu Arg Gly

2555 2560 2565

Gln Val Gly Phe Gly Pro Ala Val Ala Asp Leu Leu Ala Gln Gly

2570 2575 2580

His Gly Val Phe Val Glu Val Ser Ala His Pro Val Leu Val Gln

2585 2590 2595

Pro Ile Thr Glu Ile Val Asp Asp Ala Asp Ser Thr Ala Val Val

2600 2605 2610

Thr Gly Ser Leu Arg Arg Glu Glu Gly Gly Pro Arg Arg Leu Leu

2615 2620 2625

Ala Ser Val Ala Glu Leu Phe Val Arg Gly Val Thr Val Asp Trp

2630 2635 2640

Ala Arg Leu Leu Pro Ala Gly Ala Ala Ser Thr Arg Ala Asp Leu

2645 2650 2655

Pro Thr Tyr Ala Phe Asp His Arg His Tyr Trp Leu Lys Pro Ala

2660 2665 2670

Ala Gln Gly Thr Asp Ala Ala Ala Leu Gly Leu Ala Gly Ala Glu

2675 2680 2685

His Pro Leu Leu Gly Ala Val Val Arg Leu Pro Gln Thr Asp Gly

2690 2695 2700

Leu Val Phe Thr Ser Arg Leu Ser Leu Arg Thr His Pro Trp Leu

2705 2710 2715

Ala Asp His Ala Val Ser Gly Val Val Leu Val Pro Gly Thr Gly

2720 2725 2730

Leu Val Glu Leu Ala Val Arg Ala Gly Asp Glu Ala Gly Cys Gly

2735 2740 2745

Val Leu Glu Glu Leu Val Ile Glu Ala Pro Leu Val Val Pro Glu

2750 2755 2760

His Gly Gly Val Arg Val Gln Val Ala Val Gly Gly Pro Gly Glu

27652770 2775

Thr Gly Ala Arg Thr Val Glu Val Phe Ser Gln Arg Glu Asp Ala

2780 2785 2790

Ala Asp Asp Gly Gly Ala Asp Ala Trp Thr Arg His Ala Thr Gly

2795 2800 2805

Leu Leu Ala Thr Thr Gly Arg Pro Gly Ala Gly Gly Ser Gly Tyr

2810 2815 2820

Asp Phe Ala Ala Trp Pro Pro Pro Gly Ala Glu Pro Val Asp Ile

2825 2830 2835

Ser Asp Gly Tyr Gly Leu Leu Ala Arg Gly Gly Tyr Gly Tyr Gly

2840 2845 2850

Pro Ala Phe Gln Gly Val Arg Ala Val Trp Arg Arg Gly Asp Glu

2855 2860 2865

Val Phe Ala Glu Val Ala Leu Pro Glu Glu Gln Arg Glu Glu Ala

2870 2875 2880

Gly Arg Tyr Gly Ile His Pro Ala Leu Leu Asp Ala Ala Leu His

2885 2890 2895

Ser Thr Met Leu Asn Ala Val Ala Ala Leu Thr Ala Ala Ala Asp

2900 2905 2910

Gly Ala Pro Asp Arg Glu Asp Gly Leu Glu Leu Arg Leu Pro Phe

2915 2920 2925

Ala Trp Asn Gly Leu Glu Leu His Ala Ala Gly AlaSer Ala Leu

2930 2935 2940

Arg Val Arg Val Ala Arg Pro Glu Pro Asp Ala Leu Ser Leu Glu

2945 2950 2955

Ala Val Asp Glu Ala Gly Gly Pro Val Val Thr Met Glu Ser Leu

2960 2965 2970

Val Gly Arg Val Val Ser Thr Glu Gln Leu Ala Ala Ala Ser Asp

2975 2980 2985

Thr Ala Arg Val Asp Ser Leu Tyr Arg Val Glu Trp Thr Glu Leu

2990 2995 3000

Pro Pro Ala Arg Glu Ala Gly Pro Ala Pro Ser Arg Val Thr Val

3005 3010 3015

Ala Thr Ala Glu Glu Val Ala Thr Leu Ala Asp Asp Val Glu Ser

3020 3025 3030

Gly Ala Ala Ala Ala Pro Ala Ala Ala Val Leu Asp Ala Val Gly

3035 3040 3045

Gly Asp Glu Glu Asp Ala Ala Leu Ser Leu Ala Ser Arg Val Leu

3050 3055 3060

Asp Val Val Gln Cys Trp Leu Ala Gly Thr Gly Leu Glu Glu Ser

3065 3070 3075

Arg Leu Leu Val Val Thr Arg Gly Ala Val Pro Ala Gly Asp Gly

3080 3085 3090

Ala Val Thr Asp Pro Ala Gly Ala Ala Val Trp Gly Leu Val Arg

3095 3100 3105

Ala Ala Gln Ala Glu Asn Pro Asp Arg Ile Val Leu Leu Asp Thr

3110 3115 3120

Asp Thr Asp Thr Asp Thr Asp Ala Gly Thr Asp Thr Asp Ala Gly

3125 3130 3135

Thr Asp Thr Asp Thr Gly Ile Asp Phe Asp Ala Asp Thr Glu Arg

3140 3145 3150

Val Leu Gly Thr Val Leu Ala Ser Gly Glu Pro Gln Leu Ala Val

3155 3160 3165

Arg Gly Thr Arg Phe Ser Val Pro Arg Leu Thr Arg Ala Thr Gly

3170 3175 3180

Gln Val Pro Asp Val Pro Ala Val Phe Gly Pro Asp Ser Thr Val

3185 3190 3195

Leu Ile Ser Gly Ala Gly Ser Leu Gly Ala Leu Ala Ala Arg His

3200 3205 3210

Leu Val Thr Arg His Gly Val Arg Arg Leu Leu Leu Ala Ser Arg

3215 3220 3225

Arg Gly Arg Asp Ala Glu Gly Val Pro Glu Leu Val Ala Glu Leu

3230 3235 3240

Thr Glu Gln Gly Ala Thr Val Ser Ala Val Ala Cys Asp Val Ser

3245 3250 3255

Asp Arg Asp Gln Val Lys Ala Leu Leu Ala Ser Val Pro Asp Glu

3260 3265 3270

Gln Arg Leu Thr Gly Ile Ile His Thr Ala Gly Val Thr Asp Asp

3275 3280 3285

Gly Val Ile Gly Ala Leu Thr Pro Glu Arg Leu Glu Arg Val Phe

3290 3295 3300

Ala Pro Lys Val Asp Ala Val Arg His Leu Asp Glu Leu Thr Arg

3305 3310 3315

Glu Leu Gly Leu Asp Leu Asp Ala Phe Val Val Tyr Ser Ser Val

3320 3325 3330

Ser Gly Val Phe Met Gly Ala Gly Ser Gly Ser Tyr Ala Ala Ala

3335 3340 3345

Asn Ala Tyr Leu Asp Gly Leu Met Ala Asn Arg Arg Ala Ala Gly

3350 3355 3360

Leu Pro Gly Leu Ser Leu Ala Trp Gly Leu Trp Glu Gln Asn Thr

3365 3370 3375

Gly Met Ala Ala His Thr Asp Asp Leu Thr Lys Ser Arg Met Ser

3380 3385 3390

Arg Arg Gly Gly Leu Thr Ala Met Thr Pro Thr Glu Gly Met Glu

3395 3400 3405

Leu Phe Asp AlaAla Val Ala Ser Gly Gln Ala Leu Leu Val Pro

3410 3415 3420

Ala Arg Leu Asp Leu Arg Gly Val Arg Ala Gly Ala Ala Ala Gly

3425 3430 3435

Gly Gly Val Pro His Leu Leu Arg Gly Leu Val Arg Ala Gly Arg

3440 3445 3450

Gln Leu Ala His Thr Ala Gly Gly Ala Gly Glu His Arg Gln Leu

3455 3460 3465

Ala Glu Arg Leu Ala Ala Leu Pro Ala Ala Glu Arg Ala Lys Ala

3470 3475 3480

Leu Leu Asp Leu Val Arg Ala Gln Val Ala Ala Val Leu Gly His

3485 3490 3495

Gly Thr Thr Tyr His Ile Asp Ala Asp Gln Gly Leu Phe Glu Ile

3500 3505 3510

Gly Phe Asp Ser Leu Thr Ala Ile Glu Leu Arg Asn Arg Leu Arg

3515 3520 3525

Ala Leu Thr Glu Arg Arg Leu Ala Pro Ser Leu Val Phe Asp His

3530 3535 3540

Pro Thr Pro Gly Met Leu Ala Ala His Leu His Ala Leu Met Tyr

3545 3550 3555

Gly Ala Gln Ala Ala Ala Pro Val Pro Leu Ser Val

3560 35653570

<210>15

<211>119

<212>PRT

<213>Streptomyces spectabilis

<400>15

Met Ser Asp Thr Ala Ala Ala Gly Arg Pro Phe Gly Ile Arg Asp Asp

1 5 10 15

Arg Ala Arg Gly Val Leu Leu Ala Glu Glu Asp Gly Gln Thr Val Gly

20 25 30

Arg Ile Val Tyr Phe Thr Leu Ala Ala Pro Glu Pro Ala Leu Val Pro

35 40 45

Val His Thr Glu Val Pro Pro Ala His Glu Gly Arg Gly Ile Ala Ser

50 55 60

Ala Leu Ala Thr Glu Leu Tyr Ala Leu Ala Ala Arg Glu Asp Arg Ala

65 70 75 80

Val Val Pro Leu Cys Pro Tyr Val Ala Lys Trp Ala Glu Arg His Gln

85 90 95

Val Pro Ala Pro Ser Ala Ala Leu Val Glu Ala Ala Leu Ala Lys Val

100 105 110

Glu Asp Asp Pro Ser Thr Trp

115

<210>16

<211>204

<212>PRT

<213>Streptomyces spectabilis

<400>16

Val Pro Val Phe Asp Ala Leu Arg Asp Gln Asp Gln Pro Gly Leu Ala

1 5 10 15

Leu Cys His Leu Val His Glu Gly Leu Leu Asp Arg Ala Arg Asp Glu

20 25 30

Gly Pro Asp Ala Val Ala Asp Glu Leu Gly Gln Ile Leu Glu Arg Ala

35 40 45

Val Ala Asp Gly Ala Gly Ala Val Leu Cys Thr Cys Ser Thr Ile Gly

50 55 60

Gly Val Ala Glu Ala Arg Ala Ala Ala Leu Gly Val Pro Val Leu Arg

65 70 75 80

Val Asp Arg Pro Met Ala Ala Ala Ala Val Thr Glu Gly Pro Ala Val

85 90 95

Thr Val Leu Ala Thr Val Thr Ser Thr Leu Ala Pro Thr Ala Ala Leu

100 105 110

Leu Ala Glu Glu Ala Glu Arg Ala Gly Arg Arg Ile Asp Val Arg Thr

115 120 125

Val Val Val Asp Gly Ala Trp Glu Arg Phe Glu Ala Gly Asp Arg Asp

130 135 140

Gly Tyr Leu Arg Leu Ile Ala Ala Ala Val Glu Arg Ala Asp AlaAsp

145 150 155 160

Gly Ala Asp Ala Val Val Leu Ala Gln Ala Ser Met Ala Asp Ala Ala

165 170 175

Ala Arg Cys Ala Val Arg Val Pro Val Leu Ala Ser Pro Arg Thr Gly

180 185 190

Leu Arg Ala Ala Ala Leu Cys Val Pro Arg Gly Ser

195 200

<210>17

<211>107

<212>PRT

<213>Streptomyces spectabilis

<400>17

Val Asp Ser Ala Arg Val Gly Thr Arg Ala Gly Arg Glu Trp Gly Thr

1 5 10 15

Ala Ala Ser Ala Ala Pro Arg Ala Ala Val Pro Pro Glu Gly Ala Gly

20 25 30

Arg Leu His Pro Pro Leu Gly Gly Ser Met Thr Asn Pro Phe Pro Asp

35 40 45

Pro Val Gln Pro Gly Pro Thr Pro Gly Pro Arg Pro Glu Pro Pro Pro

50 55 60

Gly Pro Pro Pro Gly Pro Gly Pro Ala Pro Gly Pro Val Pro Val Pro

65 70 75 80

Gln Pro Pro Pro Ser Pro Val Pro Lys Pro Pro Gly Pro Asp Pro Val

85 90 95

Pro Asp Pro Asp Pro Thr Pro Glu Pro Met Ser

100 105

<210>18

<211>478

<212>PRT

<213>Streptomyces spectabilis

<400>18

Met Ser Ser Ser Ala Gln Ile Gly Val Thr Gly Leu Ala Val Met Gly

1 5 10 15

Arg Asn Leu Ala Arg Asn Phe Ala Arg Asn Gly Phe Thr Val Ala Val

20 25 30

His Asn Arg Thr Ala Ala Lys Ala Arg Ala Leu Val Asp Glu Phe Gly

35 40 45

Asp Glu Gly Glu Phe Val Tyr Thr Glu Thr Ala Lys Asp Phe Val Ala

50 55 60

Ala Leu Glu Arg Pro Arg Arg Leu Val Val Met Val Lys Ala Gly Glu

65 70 75 80

Pro Thr Asp Ala Val Ile Lys Glu Phe Ala Pro Leu Leu Glu Pro Gly

85 90 95

Asp Met Ile Val Asp Gly Gly Asn Ala His Phe Ala Asp Thr Arg Arg

100 105 110

Arg Glu Arg Glu Leu Arg Glu Leu Gly Ile His Phe Val Gly Thr Gly

115 120 125

Val Ser Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser Ile Met Pro

130 135 140

Gly Gly Ser Ala Glu Ser Tyr Ala Ala Leu Gly Pro Met Leu Glu Lys

145 150 155 160

Ile Ser Ala Lys Ala Ala Asp Gly Ser Pro Cys Thr Ala His Ile Gly

165 170 175

Pro Asp Gly Ala Gly His Phe Val Lys Met Val His Asn Gly Ile Glu

180 185 190

Tyr Ala Asp Met Gln Leu Ile Ala Glu Ala Tyr Gln Leu Leu Arg Glu

195 200 205

Val Ala Gly Tyr Ser Pro Ala Glu Ile Ala Asp Ile Phe Arg Thr Trp

210 215 220

Asn Arg Gly Arg Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala Glu Val

225 230 235 240

Leu Ala His Val Asp Ala Ala Thr Gly Lys Pro Phe Val Asp Val Val

245 250 255

Ala Asp Arg Ala Glu Gln Lys Gly Thr Gly Arg Trp Thr Val Gln Ile

260 265270

Ala Leu Asp Leu Gly Val Pro Val Ser Gly Ile Ala Glu Ala Val Phe

275 280 285

Ala Arg Ser Val Ser Gly His Thr Glu Leu Arg Ala Ala Ser Arg Asp

290 295 300

Leu Ala Gly Pro Val Ala Glu Pro Leu Ser Lys Glu Ala Ala Ala Ala

305 310 315 320

Phe Ala Asp Arg Val Glu Gln Ala Leu Tyr Ala Ser Lys Ile Val Ser

325 330 335

Tyr Thr Gln Gly Phe His Glu Ile Ala Ala Gly Ser Ala Glu Tyr Gly

340 345 350

Trp Asp Ile Asp Leu Gly Ala Val Ala Ser Ile Trp Arg Gly Gly Cys

355 360 365

Ile Ile Arg Ala Ala Phe Leu Asp Arg Ile Arg Ala Ala Tyr Asp Ala

370 375 380

Arg Ala Asp Leu Pro Ser Leu Leu Ala Asp Glu Gly Phe Ala Arg Glu

385 390 395 400

Ile Gly Ala Ala Gln Asp Asp Trp Arg Ala Val Leu Val Ala Ala Thr

405 410 415

Gln Gln Gly Val Pro Ala Pro Gly Phe Ala Ala Ala Leu Ala Tyr Tyr

420 425430

Asp Ala Leu Arg Ala Glu Arg Leu Pro Ala Ala Leu Thr Gln Gly Gln

435 440 445

Arg Asp Tyr Phe Gly Ala His Thr Tyr Arg Arg Thr Asp Arg Glu Gly

450 455 460

Ser Phe His Thr Leu Trp Gly Gly Asp Arg Ser Glu Val Glu

465 470 475

<210>19

<211>323

<212>PRT

<213>Streptomyces spectabilis

<400>19

Val Pro His Phe Asp Leu Pro Leu Asp Gln Leu Arg Gln Tyr His Pro

1 5 10 15

Asp Leu Pro Glu Pro Glu Asp Phe Asp Ala Phe Trp Glu Lys Thr Leu

20 25 30

Ala Ala Ala Arg Glu His Asp Leu Asp Val Arg Phe Glu Pro Val Asp

35 40 45

Val Pro Leu Ala Ser Val Asp Val Phe Asp Val Thr Phe Ala Gly Phe

50 55 60

Gly Gly His Pro Val Lys Gly Trp Leu Leu Leu Pro Ala Gly Ala Arg

65 70 75 80

Glu Pro Leu Pro Ala Val Val Glu Tyr Ile Gly Tyr Gly Gly Gly Arg

85 90 95

Gly Leu Pro His Glu Arg Leu Leu Trp Ser Ala Ala Gly Phe Ala His

100 105 110

Phe Ile Met Asp Thr Arg Gly Gln Gly Ser Gly Gly Ser Val Gly Asp

115 120 125

Thr Pro Asp Pro Val Gly Ser Ala Pro Ala Tyr Pro Gly Phe Leu Thr

130 135 140

Arg Gly Val Glu Asp Pro His Glu Tyr Tyr Tyr Arg Arg Val Phe Thr

145 150 155 160

Asp Ala Val Arg Ala Val Glu Ala Val Arg Ala His Pro Leu Val Asp

165 170 175

Ala Ala Arg Val Ala Ala Val Gly Asp Ser Gln Gly Gly Gly Ile Thr

180 185 190

Leu Ala Val Gly Gly Leu Val Pro Asp Leu Ala Ala Ile Ser Pro Asn

195 200 205

Val Pro Phe Leu Cys Asp Phe Pro Arg Ala Ala Thr Leu Thr Ala Arg

210 215 220

Pro Pro Tyr Arg Glu Ile Gly Leu Tyr Leu Gly Ala Arg Arg Gly Arg

225 230 235 240

Glu Asp Arg Val Phe Arg Thr Leu Ser Tyr Phe Asp Gly Val His Phe

245 250 255

Ala Ala Arg Gly Arg Ala Pro Ala Leu Phe Ser Ala Ala Leu Glu Asp

260 265 270

Glu Thr Cys Pro Pro Ser Thr Val Phe Ala Ala Phe Asn Ala Tyr Ala

275 280 285

Gly Ser Glu Arg Ser Met Glu Val Tyr Arg Phe Asn Asp His Glu Gly

290 295 300

Gly Gly Val Phe Leu Gln Asp Val Gln Leu Arg Trp Leu Pro Gly His

305 310 315 320

Leu Arg Asn

<210>20

<211>445

<212>PRT

<213>Streptomyces spectabilis

<400>20

Met Ala Leu Arg Gly Arg His Arg Arg Tyr Gln Pro Ser Arg Ile Asn

1 5 10 15

Arg Ala Ser Leu Thr Val Thr Ala Gly Gly Ala Gly Leu Ala Leu Pro

20 25 30

Leu Val Ser Ala Gly Thr Gly His Ala Ala Asp Val Asp Thr Trp Asn

35 40 45

Lys Val Ala Ala Cys Glu Ser Thr Asn Asn Trp Ser Ile Asn Thr Gly

50 55 60

Asn Gly Phe Tyr Gly Gly Leu Gln Phe Thr Gln Ser Thr Trp Glu Ala

65 70 75 80

Tyr Gly Gly Thr Ala Tyr Ala Pro Arg Ala Asp Leu Ala Thr Lys Asp

85 90 95

Gln Gln Ile Ala Val Ala Glu Lys Val Leu Asp Gly Gln Gly Pro Gly

100 105 110

Ala Trp Pro Val Cys Ser Val Arg Ala Gly Leu Thr Arg Gly Gly Asp

115 120 125

Thr Pro Asp Ile Lys Pro Ala Thr Ala Pro Gln Lys Arg Thr Gly Thr

130 135 140

Ala Asn Asp Ala Arg Ala Glu Gly Gly Arg Thr Ala Pro Arg Pro Pro

145 150 155 160

Gln Arg Thr Val Lys Asp Val Lys Pro Glu Ser Thr Pro Gln Ser Ala

165 170 175

Ala Gly Thr Ala Arg Met Tyr Thr Val Val Arg Gly Asp Thr Leu Ser

180 185 190

Gly Ile Ala Asp Glu Arg Arg Val Arg Gly Gly Trp Pro Gln Leu Tyr

195 200 205

Gln Asn Asn Arg Arg Ala Ile Gly Asp Asp Pro Asp Leu Ile Thr Pro

210 215 220

Gly Gln Arg Leu Ser Leu Arg Gly Ala Asp Gly Lys Glu Lys Arg Pro

225 230 235 240

Ala Arg Pro Pro Glu Arg Arg Lys Pro Glu Gly Thr Glu Arg Pro Asp

245 250 255

Lys Arg Pro Val Arg Ala Pro Glu Lys Gln Arg Ser His Pro Gln Ala

260 265 270

Lys Pro Gln Glu Arg Lys Gln Ala Lys Pro Gln Glu Arg Lys Gln Ala

275 280 285

Arg Pro Ala Pro Lys Arg Asp Thr Ala Pro Ala Gly Gln Arg Lys Ala

290 295 300

Lys Pro Ala Ala Gln Ala Gln Ser Gly Ala Thr Ala Pro Val Ala Ala

305 310 315 320

Ser Ile Gly Thr Pro Tyr Arg Ala Ser Gly Ser Ser Trp Ser Lys Gly

325 330 335

Tyr His Thr Gly Val Asp Phe Pro Val Pro Thr Gly Thr Ala Val Lys

340 345 350

Ala Val Ala Ala Gly Thr Val Val Glu Ser Gly Trp Gly Gly Ser Tyr

355 360 365

Gly Tyr Gln Val Val Ile Arg His Ala Asp Gly Arg Tyr Ser Gln Tyr

370 375 380

Gly His Met Ser Ala Leu Ser Val Lys Ala Gly Gln Lys Val Val Pro

385 390 395 400

Gly Gln Arg Ile Gly Arg Ser Gly Ser Thr Gly Asn Ser Ser Gly Pro

405 410 415

His Leu His Phe Glu Ile Arg Thr Gly Pro Gly Phe Gly Ser Asp Val

420 425 430

Asp Pro Leu Ala Tyr Leu Arg Ala Arg Gly Val Arg Ile

435 440 445

<210>21

<211>405

<212>PRT

<213>Streptomyces spectabilis

<400>21

Met Ser Ala Leu Ala Leu Ser Val Leu Leu Ser Leu Val Ser Ala Val

1 5 10 15

Ala Tyr Ala Gly Gly Ala Ile Leu Gln Glu Gln Val Ala Ala Thr Ala

20 25 30

Ser Gln Tyr Gln Tyr Ala Pro Leu Arg Arg Gly Val Trp Trp Val Ala

35 40 45

Val Val Leu Asn Gly Leu Gly Gly Leu Leu His Val Val Ala Leu Ala

50 55 60

Tyr Gly Pro Leu Ser Leu Val Gln Pro Leu Gly Ala Leu Thr Ile Val

65 70 75 80

Phe Ala Leu Pro Met Ala Ala Met Phe Val Arg Arg Lys Ala Gly Ala

85 90 95

Thr Ala Trp Arg Gly Ala Leu Met Ala Thr Leu Gly Leu Ala Gly Leu

100 105 110

Leu Ser Leu Thr Gln Ala Ala Asp Ser Arg Ser Leu Ala Asp Thr Glu

115 120 125

Arg Val Met Leu Ala Leu Gly Thr Gly Gly Ala Val Val Cys Leu Met

130 135 140

Leu Leu Ala His Ala Ala His Arg His Pro Ala Val Arg Ser Val Leu

145 150 155 160

Leu Ala Thr Ala Ala Gly Ile Ala Phe Gly Ile Ser Ser Val Phe Thr

165 170 175

Lys Thr Val Ala Val Asp Trp Asp Ala His Glu Pro Leu Thr Arg Gln

180 185 190

Val Pro Ser Leu Ala Val Ile Ala Val Leu Ala Val Ala Gly Leu Leu

195 200 205

Leu Ser Gln Ala Ser Tyr Arg Gly Ala Gly Leu Ala Ala Pro Leu Ser

210 215 220

Thr Val Thr Val Val Asn Pro Val Ile Ala Ala Ala Val Gly Leu Thr

225230 235 240

Leu Phe Gly Glu Ser Phe Arg Tyr Gly Ala Val Gly Thr Ala Val Ala

245 250 255

Leu Thr Cys Gly Val Val Ala Ala Gly Gly Leu Ile Leu Leu Thr Thr

260 265 270

Glu Arg Ile Gly Ala Glu Ala Ala Ser Ala Ala Ala Arg Asp Asp Ala

275 280 285

Ala Pro Ala Ala Pro Arg Asp Asp Arg Pro Ala Ala Glu Pro Val Ala

290 295 300

Arg Ser Val Pro Thr Pro Ser Thr Ala Ser Pro Thr Ser Pro Val Ser

305 310 315 320

Pro Ile Ser Pro Val Ser Pro Gly Ala Ala Val Ser Pro Ala Ser Ala

325 330 335

Ala Ser Glu Glu Thr Ala Thr Val Thr Glu Gln Arg Val Pro Asp His

340 345 350

Asp Glu Arg Pro Gly Ala Asp Ala Gly Thr Arg Ser Arg Pro Gly Glu

355 360 365

Gln Ala Ala Pro Glu Gln Ser Phe Leu Pro Glu Gly Leu Ala Gly Leu

370 375 380

Ala Gly Val Pro Met Gln Pro Val Leu Gln Thr Phe Pro Arg Ala Asp

385390 395 400

Ala Arg Ala Ala Arg

405

<210>22

<211>238

<212>PRT

<213>Streptomyces spectabilis

<400>22

Val Val Gly Ser Val Gly Gly Phe Phe Ala Leu Arg Thr Gly Glu Pro

1 5 10 15

Pro Gly Arg Thr Leu Pro Met Val Ala Arg Val Tyr Glu Ile Gly Arg

20 25 30

Val Val Thr Glu Gly Asp Pro Leu Ser Phe Arg Val Thr Lys Val Ala

35 40 45

Ala Arg Leu Ala Ala Pro Glu Pro Arg Val Ala Ala Ser Ile Ala His

50 55 60

Leu Gly Leu Ala Ala Arg Leu Trp Ser Leu Ala Leu Gly Pro Ala Ala

65 70 75 80

Leu Tyr Gly Arg Val Pro Asp Leu Asp Pro Arg Arg Leu Arg Trp Asp

85 90 95

Ala Asp Gly Ser Ser Pro Asp Asp Leu Trp Leu Ser Gly Asp Arg Thr

100 105 110

Phe Pro Ala Asp Ala Ala Arg Ile Arg Glu Val Val Leu Glu Gly His

115 120 125

Leu Ala Pro Leu Ala Ala Ala Leu Arg Ala Arg Tyr Arg Ile Ser Pro

130 135 140

Ala Leu Leu Arg Gly Asn Ala Gly Ser Ala Leu Ala Gly Ala Ala Arg

145 150 155 160

Glu Leu His Ala Trp Ala Glu Arg Thr Gly Arg Pro Glu Ala Gly Arg

165 170 175

Arg Ala Leu Ala Leu Ala Thr Glu Leu Phe Thr Ala Pro Glu Leu Arg

180 185 190

Gly Thr Gly Thr Leu Asp Gly Thr Ala Phe Arg Arg Arg Ser Cys Cys

195 200 205

Leu Tyr Tyr Arg Cys Pro Asn Gly Gly Leu Cys Gly Asp Cys Cys Phe

210 215 220

Asp Arg Pro Pro Gln Arg Ser Ser Leu Glu Ala Pro Ala Gly

225 230 235

<210>23

<211>349

<212>PRT

<213>Streptomyces spectabilis

<400>23

Val Ala Ala Ala Asp Ala Ala Val Arg His Arg Ala Trp Ser Ala Leu

1 5 10 15

Asp Thr Ala Asn Asp Ala Leu ArgThr Leu Ser Val Asp Leu Ala Met

20 25 30

Ala Ala Ala Leu Asp Gly Arg Asp Leu Val His Thr His Thr Trp Tyr

35 40 45

Ala Asn Leu Ala Gly His Val Gly Lys Leu Leu His Gly Ile Pro His

50 55 60

Val Met Thr Ser His Ser Leu Glu Pro Leu Arg Pro Trp Lys Ala Glu

65 70 75 80

Gln Leu Gly Gly Gly Tyr Ala Val Ser Ser Trp Ala Glu Arg Thr Ala

85 90 95

Val Glu Ala Ala Asp Ala Val Ile Ala Val Ser Gly Ala Met Arg Glu

100 105 110

Asp Ile Leu Ala Cys Tyr Pro Ala Val Asp Pro Ala Lys Val Arg Val

115 120 125

Val His Asn Gly Ile Asp Thr Glu Leu Tyr Arg Pro Asp Pro Gly Thr

130 135 140

Ser Ala Leu Val Arg His Gly Val Asp Pro Thr Arg Pro Tyr Val Leu

145 150 155 160

Phe Val Gly Arg Ile Thr Arg Gln Lys Gly Val Pro His Leu Leu Arg

165 170 175

Ala Ala Arg Cys Leu Glu Pro Gly Val Gln Leu Val Leu Cys Ala Gly

180 185 190

Ala Pro Asp Thr Pro Glu Ile Asp Arg Glu Phe Arg Ala Leu Phe Glu

195 200 205

Glu Leu Ser Arg Ala Arg Ala Gly Val His Trp Ile Pro Arg Met Leu

210 215 220

Pro Arg Pro Glu Val Val Gln Leu Leu Ser His Ala Ala Val Phe Ala

225 230 235 240

Cys Pro Ser Val Tyr Glu Pro Leu Gly Ile Val Asn Leu Glu Ala Met

245 250 255

Ala Cys Gly Thr Ala Val Val Ala Ser Arg Val Gly Gly Ile Pro Glu

260 265 270

Val Val Ala Asp Gly Thr Thr Gly Leu Leu Val Pro Tyr Asp Arg His

275 280 285

Asp Glu Glu Arg Phe Glu His Asp Leu Gly Leu Leu Leu Asn His Leu

290 295 300

Ala Ala Asp Pro Arg Arg Ala Ala Ala Met Gly Ala Ala Gly Arg Tyr

305 310 315 320

Arg Ala Arg Arg Glu Phe Gly Trp Gly Thr Val Ala Arg Arg Thr Val

325 330 335

Glu Val Tyr Glu Asp Val Leu Gly Val Leu Lys Ala Thr

340 345

<210>24

<211>406

<212>PRT

<213>Streptomyces spectabilis

<400>24

Met Arg Gly Gly Pro Ser Val Leu Gly Ile Val Leu Ala Gly Gly Ala

1 5 10 15

Gly Lys Arg Leu Leu Pro Leu Thr Ala Asp Arg Ala Lys Pro Ala Val

20 25 30

Thr Phe Gly Gly Thr Tyr Arg Leu Val Asp Phe Val Leu Ser Asn Leu

35 40 45

Val Asn Gly Asp Val Leu Arg Ile Cys Val Leu Thr Gln Tyr Lys Ser

50 55 60

His Ser Leu Asp Arg His Val Ser Thr Thr Trp Arg Met Ser Ser Leu

65 70 75 80

Leu Gly Asn Tyr Val Thr Pro Val Pro Ala Gln Gln Arg Leu Gly Pro

85 90 95

Arg Trp Tyr Leu Gly Ser Ala Asp Ala Ile Leu Gln Ser Leu Asn Leu

100 105 110

Val His Asp Glu Gln Pro His Tyr Val Ala Val Phe Gly Ala Asp His

115 120 125

Val Tyr Arg Met Asp Pro Arg Gln Met Leu Glu Gln His Val Glu Ser

130 135 140

Gly Ala Gly Val Thr Val Ala Gly Ile Arg Val Pro Arg Ala Asp Ala

145 150 155 160

Arg Gln Phe Gly Val Ile Thr Pro Ala Arg Asp Gly Thr Leu Val Glu

165 170 175

Arg Phe Leu Glu Lys Pro Gln Asp Pro Pro Gly Leu Pro Gly Ala Pro

180 185 190

His Gln Val Tyr Ala Ser Met Gly Asn Tyr Ile Phe Thr Thr Lys Ala

195 200 205

Leu Val Asp Ala Leu Arg Gln Asp Ala Asp Asp Glu Asp Ser Ala His

210 215 220

Asp Met Gly Gly Ser Ile Leu Pro Met Leu Thr Glu Arg Gly Leu Ala

225 230 235 240

Gln Val Tyr Asp Phe Asp Gly Asn His Val Pro Gly Glu Thr Ala Arg

245 250 255

Asp His Gly Tyr Trp Arg Asp Val Gly Thr Leu Asp Ala Tyr Tyr Glu

260 265 270

Ala His Met Asp Leu Ile Ser Asp Gln Pro Val Phe Asn Leu Asp Asn

275 280 285

Arg Cys Trp Pro Ile Tyr Thr His Pro Gly Gln Leu Pro Pro Ala Lys

290 295 300

Phe Cys Ala Gly Gly Ile Ala Gly Glu Ser Ile Val Ser Pro Gly Cys

305 310 315 320

Val Ile Arg Gly Gln Val Thr Arg Ser Val Leu Ser Pro Gly Val Thr

325 330 335

Val Glu Glu Gly Ala Val Val Gln Gly Ser Val Leu His Asp Asn Val

340 345 350

Arg Val Gly Arg Gly Ala Val Val Arg Gly Ala Val Leu Asp Lys Asn

355 360 365

Val Asp Val Pro Pro Gly Ala Thr Ile Gly Val Asn Pro Glu Arg Asp

370 375 380

Ala Glu Leu Tyr Thr Val Ser Arg Ala Gly Val Ile Ala Leu Gly Lys

385 390 395 400

Gly Gln Cys Val Pro Glu

405

<210>25

<211>322

<212>PRT

<213>Streptomyces spectabilis

<400>25

Val Ala Leu Ile Gly Ala Gly Leu Ile Ala Arg Leu His Leu Asp Ala

1 5 10 15

Trp Ile Ser Thr Gly Ala Ser Val Arg Val His Ser Lys Asp Gly Arg

20 25 30

Ala Pro Asp Leu Ala Arg Ala Cys Gly Ala Arg Ala Val Asp Thr Leu

35 40 45

Asp Glu Ala Leu Asp Gly Ala Asp Val Val Asp Ile Cys Ser Pro Thr

50 55 60

Ser Ser His His Glu Ile Ala Met Ala Ala Val Ala Ala Gly Val Gly

65 70 75 80

Ile Val Cys Glu Lys Pro Leu Ala Ala Asp Val Thr Glu Ala Glu Glu

85 90 95

Ile Val Thr Ala Ala Glu Arg Ala Gly Val Arg Leu Cys Pro Thr His

100 105 110

Asn Ala Arg Phe Ala Pro Ala Phe Ala Arg Leu His Asp Leu Val Ala

115 120 125

Ala Gly Gly Ile Gly Ala Gly Ala Val Ala Thr Phe Asp Val Ala Gly

130 135 140

Tyr His Pro Arg Pro Trp Thr Gly Arg Ala Ser Ala Arg Ser Gly Gly

145 150 155 160

Ile Leu Thr Asp Gln Met Leu His Gly Val Asp Ile Ala Tyr Trp Ile

165 170 175

Phe Gly Asp Val Val Arg Val His Ala His Tyr Gln Gly Gln Leu Thr

180 185 190

Ala Pro Ala Pro Glu Gly Ala Phe Ala Val Gly Thr Ala Val Leu Thr

195 200 205

His Ala Asp Gly Ala Leu Ser Arg Ile Val Ser Arg Trp Thr Ala Thr

210 215 220

Pro Asp Pro Pro Ile Arg Val Ala Tyr His Val Ser Gly Thr Gly Gly

225 230 235 240

Thr Val Leu His Asp Ser Glu Trp Pro Gln Glu Ile Lys Val Phe Gly

245 250 255

Gly Ala Gly Gly Ala Phe Ala Pro His Gly Glu Ser Pro Phe Leu Ala

260 265 270

Glu Ile Arg Glu Phe Ser Arg Ala Phe Gln Gly Gly Pro Glu Pro Arg

275 280 285

Leu Gly Pro Arg Asp Ala Leu Ala Ala Val Arg Ile Thr Gln Ala Ala

290 295 300

Ala Glu Ser Ala Ser Thr Gly Arg Ala Val Glu Leu Pro Val Lys Gly

305 310 315 320

Ala Ala

<210>26

<211>327

<212>PRT

<213>Streptomyces spectabilis

<400>26

Val Leu Ser Leu Ala His Glu Arg Ala Ala Ala Tyr Ala Arg Leu Leu

1 5 10 15

Arg Ala Met Pro Gly Val Asp Leu Leu Val Ala Asp Pro Glu Gly Ala

20 25 30

Pro Asp Glu Gln Gly Arg Gly Gln Ala Ala Ala Arg Arg Leu Asp Ala

35 40 45

Ala Tyr Ala Gly Ser Trp Asp Lys Ala Phe Ala Leu Arg Pro Asp Ala

50 55 60

Val Val Val Thr Ser Glu Val Ala Arg Arg Arg Glu Leu Val Glu Arg

65 70 75 80

Ala Ala Glu Ile Gly Ala His Val Leu Cys Glu Arg Pro Gln Ala Asp

85 90 95

Thr Glu Ala Asp Ala Asp Ala Met Val Arg Ala Cys Glu Asp Ala Gly

100 105 110

Val Arg Leu Thr Leu Ala Ala Pro Ala Cys Phe Ser Pro Ala Leu Ala

115 120 125

Ala Val Arg Ala Gln Leu Ala Asp Gly Ala Ala Ile Gly Pro Leu Thr

130 135 140

Thr Val His Gly Thr Tyr Tyr Gly Pro Gly Asp Arg Ala Ala Gly Gly

145 150 155 160

Ala Leu Arg Thr His Ala Pro Val Leu Leu Asp Leu Val Asp Thr Val

165 170 175

Leu Asp Gly Ala Pro Ala Ala Gln Val Tyr Ala Gln Ser Asn Ser Val

180 185 190

Leu Ser Gly Asp Pro Asp Val Glu Ser Ala Ala Leu Leu Thr Leu Arg

195 200 205

Phe Ala Asp Gly Thr Val Ala Ala Leu Asp Cys Gly Trp Ser Pro Pro

210 215 220

Gly Asn His Arg Ala Ala Ala Gly Pro Thr Leu Thr Phe Val Gly Glu

225 230 235 240

Arg Gly Ser Val Glu Phe Thr Ala Arg Pro Lys Leu Leu Gly Gly Phe

245 250 255

Asp Ser Gly Thr Ala Arg Glu Arg Trp Thr Thr Ala Val Ser Asp Pro

260 265 270

Tyr Ala Val Val Leu Asp Ala Phe Val Ala Ser Val Ala Thr Gly Arg

275 280 285

Gly Thr Gly Pro Asp Gly Ala Thr Gly Val Arg Thr Leu Arg Val Ile

290 295 300

Arg Ala Ala Tyr Glu Ser Ala Arg Thr Gly Glu Pro Val Asp Val Ala

305 310 315 320

Val Pro Gln Pro Val Arg Pro

325

<210>27

<211>187

<212>PRT

<213>Streptomyces spectabilis

<400>27

Met Thr Arg Ile Gly Ile Ile Leu Gly Thr Thr Arg Pro Gly Arg Val

1 5 10 15

Gly Pro Gln Val Ala Gln Trp Val Glu Lys Ala Ala Arg Thr Arg Gly

20 25 30

Asp Ala Asp Phe Glu Leu Val Asp Leu Ala Asp Phe Asp Leu Pro Leu

35 40 45

Phe Asp Glu Pro Arg Ser Pro Arg Met Gly Ala Tyr Glu His Ala His

50 55 60

Thr Arg Ala Trp Ser Ala Lys Ile Ala Glu Leu Asp Gly Phe Val Phe

65 70 75 80

Val Thr Pro Glu Tyr Asn Trp Ser Ile Pro Ala Ala Leu Lys Asn Ala

85 90 95

Ile Asp Phe Val Tyr Asn Glu Trp Asn Asn Lys Ala Ala Ala Phe Val

100 105 110

Gly Tyr Gly Ala Ser Ala Asn Gly Val Arg Ala Val Glu His Leu Arg

115 120 125

Ala Ile Ala Ala Ala Val Gln Leu Ser Pro Val Tyr Thr Gln Val Asn

130 135 140

Leu Ser Leu Leu Thr Asp Phe Glu Gln Tyr Glu Thr Phe Thr Pro Ala

145 150 155 160

Glu Arg His Asp Ala Ala Leu Gln Arg Met Leu Ser Glu Leu Ile Thr

165 170 175

Arg Thr His Thr Leu Ala Pro Leu Arg Arg Ala

180 185

<210>28

<211>430

<212>PRT

<213>Streptomyces spectabilis

<400>28

Met Ser Leu Pro Thr Ala Ala Pro Ala Thr Gly Pro Thr Pro Pro Asp

1 5 10 15

Asp Thr Thr Pro Ser Val Tyr Arg Leu Pro Phe Pro Pro Gly Phe Thr

20 25 30

Glu Arg Ser Gly Cys Pro Phe Asp Pro Pro Pro Met Leu Arg Asp His

35 40 45

His Asp Gln Gly Glu Val Arg Glu Ile Ala Leu Thr Asp Gly Gly Thr

50 55 60

GlyTrp Leu Val Thr Gly Tyr Glu Glu Gly Arg Thr Leu Leu Ser Asp

65 70 75 80

Pro Arg Leu Ser Ser Asp Arg Leu Arg Asn Pro Leu Val Val Lys Leu

85 90 95

Pro Ala Glu Leu Arg Ala Gln Leu Met Ala Asp Glu Ala Val Ala Gly

100 105 110

Asn Phe Ile Ala Met Asp Pro Pro Asp His Thr Arg Tyr Arg Lys Met

115 120 125

Leu Thr Arg Gln Phe Thr Leu Arg Arg Val Arg Gln Leu Glu Pro Arg

130 135 140

Ile Leu Glu Ile Val Thr Glu Arg Ile Asp Ala Met Leu Ala Arg Gly

145 150 155 160

Asn Ser Ala Asp Leu Val Lys Asp Phe Gly Leu Ala Val Pro Ser Leu

165 170 175

Val Ile Cys Glu Leu Leu Gly Val Ala Tyr Glu Glu Arg Gly Glu Phe

180 185 190

Gln Ala Arg Thr Glu Glu Leu Leu Asn Leu Asp Ile Pro Phe Glu Lys

195 200 205

Gln Leu Ala Ala Leu Asp Glu Leu Arg Lys Phe Ile Leu Gly Gln Val

210 215 220

Arg Arg Lys Arg Ala Thr Pro Thr Asp Asp Met Leu Ser Asp Leu Ile

225 230 235 240

Arg Ser Asp Thr Glu Pro Pro Leu Thr Asp Glu Glu Val Val Gly Met

245 250 255

Ala Asn Leu Leu Leu Val Gly Gly His Glu Thr Thr Ala Asn Met Leu

260 265 270

Ser Leu Gly Thr Phe Ala Leu Leu Glu His Pro Glu Gln Phe Arg Ala

275 280 285

Leu Arg Asp Arg Pro Glu Leu Met Asp Gly Ala Val Glu Glu Leu Leu

290 295 300

Arg Tyr Leu Ser Ile Val His His Gly Leu Leu Arg Thr Thr Thr Glu

305 310 315 320

Asp Val Glu Ile Ala Gly Arg Thr Ile Pro Ala Glu Ser Leu Val Ile

325 330 335

Ile Ser Val Ser Glu Ala Asn Arg Asp Pro Arg His Tyr Asp Ser Pro

340 345 350

Ala Ala Leu Asp Val Ser Arg Pro Arg Arg Thr His Leu Ala Phe Gly

355 360 365

His Gly Ile His Gln Cys Ile Gly Gln Gln Leu Ala Arg Ser Glu Met

370 375 380

Thr Ile Ala Phe Pro Glu Leu Leu Arg Arg Leu Pro Gly Leu Arg Leu

385 390 395 400

Ala Val Pro Ala Asp Glu Val Pro Leu Arg Glu Lys Met Ala Val Tyr

405 410 415

Gly Leu His Ser Leu Pro Val Thr Trp Asp Ala Pro Asp Ala

420 425 430

<210>29

<211>202

<212>PRT

<213>Streptomyces spectabilis

<400>29

Val Leu Glu Arg Met Pro Trp Asp Ile Gly Gly Pro Gln Pro Val Leu

1 5 10 15

Val Glu Leu Glu Arg Ala Gly Arg Val Ser Gly Asp Val Leu Asp Ile

20 25 30

Gly Ser Gly Val Gly Asp Asn Ala Ile His Leu Ala Arg Cys Gly Tyr

35 40 45

Arg Val Thr Gly Leu Asp Val Ala Pro Thr Ala Val Glu Lys Ala Arg

50 55 60

Ala Arg Ala Ala Glu Lys Gly Ala Asp Val Thr Phe Ala Val Gly Asn

65 70 75 80

Ala Thr Ser Leu Asp Gly Tyr Glu Asn Gln Phe Asp Thr Val Thr Ser

8590 95

Ser Leu Val Phe His Cys Phe Gly Pro Lys Gln Arg Arg Ala Tyr Ala

100 105 110

Asp Ala Ile Ala Arg Val Leu Arg Pro Gly Gly Arg Leu Leu Gln Trp

115 120 125

Cys Ser Arg Gly Ala Ala Ala Gly Pro Glu Pro Ile Thr Glu Glu Thr

130 135 140

Ile Arg Asp Ala Phe Ser Gly Pro Gly Trp Ser Ile Thr Thr Leu Arg

145 150 155 160

Ala Gln His Leu Thr Thr Thr Val Leu Asp Glu Pro Leu Arg Lys Asp

165 170 175

Tyr Glu Gly Gly Gln Leu Asp Thr Asp Asp Ser Gly Ala Thr Leu Leu

180 185 190

Pro Ile Trp Leu Leu Glu Ala Ala Arg Asp

195 200

<210>30

<211>401

<212>PRT

<213>Streptomyces spectabilis

<400>30

Met Thr Glu Thr Pro Ala Thr Pro Thr Gly Leu Pro Thr Ala Arg Ala

1 5 10 15

Ala Gly Cys Pro Phe Asp Pro Pro Pro Gly Leu Ala Ala Leu Arg Asp

20 25 30

Arg Ala Pro Leu Thr Arg Met Glu Phe Pro Asn Gly His Val Gly Trp

35 40 45

Leu Ala Thr Gly His Ala Val Val Arg Ala Val Leu Ala Asp Pro Arg

50 55 60

Phe Ser His Arg Asn Asp Arg Arg His Trp Pro Leu Ala Asp Ile Gly

65 70 75 80

Arg Gly Phe Pro Pro Leu Pro Gly Asp Met Leu His Ile Asp Pro Pro

85 90 95

Asp His Thr Arg Tyr Arg Lys Leu Leu Ala Gly Lys Phe Thr Met Arg

100 105 110

Arg Met Arg Arg Leu Thr Gly Ser Ala Gln Glu Ile Val Ala Gly Lys

115 120 125

Leu Asp Ala Met Glu Arg His Gly Gly Pro Leu Asp Leu Leu Glu Phe

130 135 140

Phe Ala Arg Pro Val Pro Thr Leu Met Val Cys Ala Leu Leu Gly Val

145 150 155 160

Pro Leu Gln Asp Arg Ala Thr Phe His Pro Pro Val Arg Gly Thr Asp

165 170 175

Asp Ala Ala Ala Val Glu Ala Asp Val Ala Ala Tyr Val Glu Met Thr

180 185 190

Tyr Ala Asp Met Gln Glu Tyr Phe Arg Lys Leu Val Ala Ala Lys Arg

195 200 205

Ala Ala Pro Ala Asp Asp Leu Leu Ser Asp Leu Thr Thr Ser Asp Leu

210 215 220

Thr Glu Asp Glu Leu Val Gly Leu Cys Ala Val Met Met His Ala Gly

225 230 235 240

Val Asp Ser Thr Ser Asn Met Leu Ala Leu Gly Thr Trp Ala Leu Leu

245 250 255

Glu Arg Pro Asp Gln Leu Ala Ala Leu Arg Glu Arg Pro Asp Leu Ala

260 265 270

Asp Arg Ala Val Glu Glu Leu Met Arg Tyr Met Ser Val Val His Thr

275 280 285

Gly Ser Arg Ala Ala Leu Glu Asp Val Glu Leu Ala Gly Glu Val Val

290 295 300

Arg Ala Gly Glu Ser Val Ala Phe Ser Val Gln Ala Ala Asn Arg Asp

305 310 315 320

Pro Ala Arg Phe Ala Asp Pro Asp Thr Leu Asp Ile Arg Arg Gly Ala

325 330 335

Val Gly His Leu Gly Phe Gly Tyr Gly Val His Gln Cys Leu Gly Met

340 345 350

Gln Leu Ala Arg Val Glu Met Arg Val Ala Phe Pro Ala Leu Phe Ala

355 360 365

Arg Phe Pro Ala Leu Arg Leu Ala Val Pro Ala Gly Asp Val Pro Met

370 375 380

Arg Asp Asp Leu Val Ile Pro Tyr Gly Val His Arg Leu Pro Val Thr

385 390 395 400

Trp

<210>31

<211>388

<212>PRT

<213>Streptomyces spectabilis

<400>31

Val Gly Tyr Thr Val Arg Thr Val Gly Arg Leu Asp Pro Ala Ala Leu

1 5 10 15

Arg Thr Ala Tyr Ala Ala Val Cys Arg Ala His Pro Gln Leu Ala Ala

20 25 30

Arg Leu Val Ala Asp Asp Gly Gly Leu Ala Phe Val Glu Ser Gly Ala

35 40 45

Pro Pro Glu Val Gln Ile Cys Ala Gly Asp Pro Asp His Pro Leu Thr

50 55 60

Gly Val Glu Leu Asp Glu Tyr Arg Met Leu Ser Ala Leu Asn Val Val

65 70 75 80

Arg Asp Gly Ser Glu Ala Ser Val Thr Leu Leu Thr His His Ser Ile

85 90 95

Ala Asp Ala His His Ser Ile Glu Leu Leu Ser Ala Leu Trp Ala Cys

100 105 110

Tyr Ala Asp Val Val Arg Gly Ala Ser Val Asp Leu Pro Arg Arg Pro

115 120 125

Tyr Pro Arg Ser Leu Glu Asp Leu Leu Ala Glu Arg Gly Ile His Arg

130 135 140

Ala Ala Ser Gly Glu Gly Gly Ala Ser Ala Pro Leu Lys Thr Val Thr

145 150 155 160

Glu Leu Pro Ala Ser Ala Arg Ile Pro Ile Val Arg His Val Val Gln

165 170 175

His Arg Leu Ser Glu Asp Gln Thr Thr Ala Leu Ala Glu Leu Gly His

180 185 190

Arg Glu Gln Val Thr Leu Asn Gly Leu Leu Ser Gly Ala Val Leu Leu

195 200 205

Val Glu Ala Glu Val Arg Gly Leu Pro Val Ser Glu Leu Leu Phe Arg

210 215 220

Tyr Thr Val Asn Leu Arg Asn Arg Leu Thr Pro Pro Ile Gly Pro Thr

225 230 235 240

Glu Gly Thr Asn Val Val Gly Gly Gly Leu Phe Lys Ala Ala Asp Asp

245 250 255

Val Glu Pro Ser Ala Val Ala Ile Gly Arg Ala Leu Gly Glu Arg Leu

260 265 270

Arg Ala Gly Leu Ala Asp Gly Ser Ile Gln Arg Ser Leu Leu Asp Met

275 280 285

Val Thr Gln Pro Ala Pro His Ala Lys Pro Arg Ala Ala Gly Pro Ala

290 295 300

Pro Ala Val Val Ser Val Ser Asn Trp Gly Val Val Pro Thr Leu Arg

305 310 315 320

Thr Pro Asp Gly Leu Leu Leu Thr Asn Phe His Ser Ser Ser Arg Arg

325 330 335

Lys Val Ala Pro Gly Glu Phe Ala Ala Ile Gly Gly Tyr Val Ala Thr

340 345 350

Thr Phe Asp Arg Arg Ile Gly Ile Asp Leu Ala Trp Pro Glu Gly Asp

355 360 365

Ala Glu Leu Pro Arg Arg Leu Asp Cys Leu Arg Gln Val Leu Asn Arg

370 375 380

Leu Thr Ser Gln

385

<210>32

<211>397

<212>PRT

<213>Streptomyces spectabilis

<400>32

Met Ser Asp Ala Ile Ser Tyr Glu Val Pro Trp Ala Arg Thr His Gln

1 5 10 15

Phe Asp Pro Pro Ala Val Phe Asp Ala Leu Arg Ala Glu Arg Pro Leu

20 25 30

Ala Arg Met Thr Tyr Pro Asp Gly His Glu Gly Trp Ile Ala Thr Gly

35 40 45

His Glu Val Val Arg Glu Val Leu Gly Asp Pro Arg Phe Ser His Ser

50 55 60

Thr Glu Ile Gly His Phe Pro Val Thr His Gln Gly Gln Leu Val Pro

65 70 75 80

Asn His Pro Lys Ile Pro Gly Met Phe Ile His Met Asp Pro Pro Asp

85 90 95

His Thr Arg Tyr Arg Arg Leu Leu Thr Gly Glu Phe Thr Val Arg Arg

100 105 110

Ala Ser Arg Leu Lys Ala Arg Val Glu Ala Val Ala Ala Glu Gln Ile

115 120 125

Asp Val Met Arg Ala His Gly Ser Pro Ala Asp Leu Val Glu Thr Phe

130 135 140

Ala Arg Pro Leu Val Leu Arg Val Leu Ser Glu Leu Val Gly Leu Pro

145 150 155 160

Tyr Asp Glu Arg Glu His Tyr Glu His Ala Pro Thr Leu Leu His Asp

165 170 175

Pro Asp Ala Asn Pro Gln Glu Val Ala Ala Ala Phe Glu Lys Ala Gly

180 185 190

Ala Phe Phe Val Glu Val Ile Glu Arg Lys Arg Lys Asn Pro Gly Asp

195 200 205

Asp Leu Leu Ser Arg Leu Thr Ala Asp Gly Gln Leu Thr Thr Glu Glu

210 215 220

Leu Cys Asn Ile Val Val Leu Leu Leu Phe Ala Gly Tyr Glu Thr Thr

225 230 235 240

Glu Ser Ala Leu Ser Val Gly Val Phe Ala Leu Leu His His Ala Glu

245 250 255

Gln Leu Ala Val Leu Arg Ala Asp Pro Glu Lys Leu Asp Thr Ala Val

260 265 270

Glu Glu Leu Leu Arg Tyr Leu Thr Val Asn Gln Tyr His Thr Tyr Arg

275 280 285

Thr Ala Leu Glu Asp Ile Glu Leu Asn Gly Glu Thr Val Lys Lys Gly

290 295 300

Asp ThrVal Thr Val Ser Leu Pro Ala Ala Asn Arg Asp Pro Ala Lys

305 310 315 320

Phe Thr Cys Pro Glu Lys Leu Asp Ile Gly Arg Asp Thr Ala Gly His

325 330 335

Val Ala Phe Gly Tyr Gly Ile His Gln Cys Leu Gly Gln Asn Leu Ala

340 345 350

Arg Val Glu Leu Arg Ala Gly Ile Ala Ala Leu Leu Gly Ala Phe Pro

355 360 365

Asp Leu Arg Leu Ala Val Ala Thr Asp Glu Val Pro Leu Arg Leu Lys

370 375 380

Gly Ser Val Phe Ala Val Lys Gly Leu Pro Val Ser Trp

385 390 395

<210>33

<211>264

<212>PRT

<213>Streptomyces spectabilis

<400>33

Val Asp Ala Ala Thr Leu Arg Lys Leu His Lys Arg His Leu Met Ala

1 5 10 15

Ile Pro Tyr Asn Gly Ala Ala Gln Asp Leu Arg Asp Gly Ile Arg Leu

20 25 30

Val Asp Leu Asp Glu Asp Ala Val Phe Glu Thr Ser Val Val Asp Gly

35 4045

His Gly Gly Thr Cys Phe Gln Leu Ala Arg Leu Phe His Arg Leu Leu

50 55 60

Ala Ala Leu Gly Tyr Asp Val Thr Leu Met Ala Gly Ser Thr Ala Glu

65 70 75 80

Gly Arg Glu Asn Phe Gly Leu Asp Val Glu His Met Phe Ser Arg Val

85 90 95

Ala Leu Asp Gly Glu Glu Trp Leu Val Asp Val Gly Tyr Pro Gly Pro

100 105 110

Asn Tyr Ile Glu Pro Leu Arg Ile Thr Asp Ala Val Gln Ser Gln Tyr

115 120 125

Gly Cys Gln Tyr Arg Leu Val Glu Asp Ala Ser Gly Phe Ala Leu Gln

130 135 140

Arg Arg Gly Arg Val Ser Arg Trp Ser Val Val Tyr Thr Phe Thr Arg

145 150 155 160

Lys Pro Arg Gln Trp Ser Asp Trp Lys Glu Leu Glu Glu Tyr Leu Asn

165 170 175

Arg Glu Tyr Ala Ala Ala Pro Ala Glu Ser Asp Ala Cys Pro Ile Ala

180 185 190

Arg Gly Thr Asp Arg Ile Phe Gly Glu Ile Glu Ala Ala Thr Gly Ala

195 200205

His Glu Gly Gln Asp Val Leu Cys Gly Arg Thr Phe Asp Asn Gly Gln

210 215 220

Val Val Leu Lys Gly Arg Arg Tyr Leu Thr Val Arg Asp Gly Arg Glu

225 230 235 240

Glu Val Arg Thr Ile Lys Asp Asp Asp Glu His Arg Asp Leu Val Phe

245 250 255

Cys Ile Leu Ser Gly Asp Phe Ser

260

<210>34

<211>414

<212>PRT

<213>Streptomyces spectabilis

<400>34

Met Thr Ala Thr Ala Glu Asn Ala Thr Glu Pro Ile Asp Leu Leu Ser

1 5 10 15

Pro Asp Leu Thr Ala Asp Pro Phe Ala Lys Tyr Ala Arg Met Arg Glu

20 25 30

Gln Ala Pro Val Leu Ala Gly Thr Val Leu Gly Ser Pro Met Trp Leu

35 40 45

Val Thr Gly Tyr Asp Asp Val Arg Leu Val Leu Thr Asp Pro Arg Phe

50 55 60

Val Thr Asp Ala Thr Ser Val Arg Gly Asp Asp Pro Asp Gly Gly His

65 7075 80

Ala Ile Leu Lys Lys Leu Asn Ile Pro Ser Asp Val Glu Asp Leu Ile

85 90 95

Ala Lys Ala Met Met Asn Leu Asp Gly Ala Pro His Ala Arg Leu Arg

100 105 110

Lys Leu Val Ser Arg Ala Phe Thr Ala Arg Arg Val Ala Glu Met Arg

115 120 125

Pro Arg Ile Glu Glu Leu Thr Ala Arg Leu Leu Asp Gly Met Ala Ala

130 135 140

Ala Gly Arg Asp Gly Thr Pro Val Asp Leu Val Glu Ala Phe Cys Tyr

145 150 155 160

Pro Val Pro Ile Ala Val Ile Cys Glu Leu Val Gly Ile Asp Glu Glu

165 170 175

Asp Arg Pro Gln Trp Ser Gly Trp Gly Arg Val Leu Ser Leu Met Gln

180 185 190

Val Glu His Leu Pro Thr Ile Leu Arg Glu Cys Ser Ala His Val Met

195 200 205

Asp Val Ile Glu Arg Arg Arg Ala Glu Pro Arg Asp Asp Leu Ile Ser

210 215 220

Ala Leu Ile Gln Ala Gln Glu Asp Asp Gly Asp Arg Leu Ser Asp Arg

225 230235 240

Glu Met Val Thr Leu Val Phe Thr Met Val Thr Ala Gly His Glu Ser

245 250 255

Thr Thr Tyr Leu Leu Gly Asn Ser Val Leu Ala Leu Leu Glu Gln Pro

260 265 270

Asp Gln Leu Ala Leu Leu Arg Ala Asp Pro Ser Arg Trp Gln Pro Ala

275 280 285

Val Arg Glu Leu Met Arg Leu Gly Pro Ser Gln Phe Ala Gln Pro Arg

290 295 300

Tyr Ala Ala Gln Asp Val Glu Leu Ser Gly Val Arg Ile Pro Ala Gly

305 310 315 320

Ser Ala Val Thr Pro Leu Leu Leu Ala Ala Asn Thr Asp Pro Arg Lys

325 330 335

Tyr Ala Asp Pro Glu Arg Leu Asp Val Gly Arg Gly Ala Ala Ser His

340 345 350

Val Gly Phe Gly Met Gly Val His His Cys Leu Gly Ala Pro Leu Ala

355 360 365

Tyr Leu Glu Ala Glu Val Ala Leu Gln Ala Leu Phe Asp Arg Phe Pro

370 375 380

Asp Leu Ser Leu Ala Val Pro Arg Glu Glu Ile Pro Trp Ala Leu Arg

385 390 395400

Pro Gly Phe Thr Arg Val Ala Glu Leu Pro Leu Arg Leu Ala

405 410

<210>35

<211>553

<212>PRT

<213>Streptomyces spectabilis

<400>35

Val Arg Phe Ser Leu Thr Pro Leu Leu Glu Val Pro Ala Asp Pro Thr

1 5 10 15

Asp Ala Asp Val Val Val Val Gly Asn Gly Pro Val Gly Ala Ala Leu

20 25 30

Ser Val Leu Leu Ala Gln Arg Gly Leu Arg Val Thr Val Leu Glu Arg

35 40 45

Arg Arg Arg Pro Tyr Pro Leu Pro Arg Ala Thr Ser Phe Asp Gly Glu

50 55 60

Thr Ala Arg Leu Leu Ala Ala Thr Gly Ile Gly Pro Asp Leu Gly Arg

65 70 75 80

Ile Thr Glu Pro Ala Asn Gly Tyr Gln Trp Arg Thr Ala Ala Gly Ala

85 90 95

Ala Leu Leu Asp Ile Pro Phe Thr Thr Ala Gly Pro Tyr Gly Trp Pro

100 105 110

Asp Ala Asn Thr Met His Gln Pro Ala Leu Glu Glu Leu Ile Ala Ala

115 120 125

Arg Ala Ala Ala Leu Gly Val Thr Val Leu Arg Gly His Glu Val Val

130 135 140

Gly Ile Ala Asp Gly Glu His Arg Val Glu Val Thr Ala Ala Thr Asp

145 150 155 160

Asp Gly Thr Thr Arg Ala Leu Ser Ala Arg Trp Val Val Gly Cys Asp

165 170 175

Gly Ala Asn Ser Phe Val Arg Asp His Leu Asp Val Pro Val Thr Asp

180 185 190

Leu Gly Phe Ser Tyr Glu Trp Leu Leu Cys Asp Val Glu Leu Arg Glu

195 200 205

Pro Arg Ala Phe Thr Pro Thr Asn Val Gln Ile Cys Asp Pro Ala Arg

210 215 220

Pro Thr Thr Leu Val Gly Ser Gly Pro Gly Arg Arg Arg Trp Glu Phe

225 230 235 240

Met Arg Leu Pro Gly Glu Arg Ala Val Glu Leu Ser Gly Asp Glu Ala

245 250 255

Ala Trp Arg Leu Leu Ala Pro Phe Gly Val Thr Pro Asp Thr Ala Thr

260 265 270

Leu Leu Arg Ser Thr Thr Tyr Ile Phe Gln Ala Ser Trp Ala Asp Gln

275 280 285

Trp Arg Val Gly His Val Leu Leu Ala Gly Asp Ala Ala His Leu Met

290 295 300

Pro Pro Phe Ala Gly Gln Gly Met Cys Ser Gly Ile Arg Asp Val Val

305 310 315 320

Asn Leu Ala Trp Lys Leu Asp Leu Thr Leu Arg Gly Gln Ala Gly Pro

325 330 335

Ala Leu Leu Asp Ser Tyr Ala Arg Glu Arg Arg Ala Gln Ala Lys Glu

340 345 350

Ala Ile Leu Ala Ser Val Gln Leu Gly Arg Leu Ile Cys Val Thr Asp

355 360 365

Pro Val Ala Ala Ala Glu Arg Asp Ala Thr Val Leu Ala Asn Arg Arg

370 375 380

Gly Arg Val Pro Ser Gly Pro Glu Pro Ala Lys Pro Leu Pro Gly Gly

385 390 395 400

Leu Leu His Arg Gly Ala Ala Ala Thr Ala Pro Ala Gly Ala Val Val

405 410 415

Pro Gln Gly Arg Val Asp Arg Gly Gly Phe Gly Gly Leu Phe Asp Asp

420 425 430

Val Val Gly Arg Gly Phe Val Leu Leu Thr Thr Glu Asp Pro Arg Ala

435 440 445

Val Leu Asp Glu Glu Arg Leu Ala Leu Leu Gly Asp Leu Ala Ala His

450 455 460

Val Val Arg Leu Leu Pro Pro Gly Ala Ala Arg Lys Asp Ala Pro Ala

465 470 475 480

Ala Val Asp Ala Ala Ala Pro Gly Val Val Glu Val Val Asp Thr Asp

485 490 495

Gly Val Tyr Arg Pro Tyr Leu Ala Arg Phe Ala Ala Thr Ala Leu Leu

500 505 510

Val Arg Pro Asp Tyr His Val Phe Gly Ala Ala Ser Gly Pro Asp Gly

515 520 525

Ile Arg Glu Leu Leu Asp Asp Leu Arg Asp Gln Leu Arg Ala Pro Val

530 535 540

Pro Ala Gly Ala Pro Arg Gly Ala Val

545 550

<210>36

<211>417

<212>PRT

<213>Streptomyces spectabilis

<400>36

Met Thr Ala Thr Ala Glu Asn Ala Thr Glu Pro Val Asp Leu Leu Ser

1 5 10 15

Pro Asp Leu Ile Ala Asp Pro Phe Ala Ala Tyr Ala Arg Met Arg Glu

20 25 30

Gln Ala Pro Thr Leu Thr Gly Ser Ile Met Gly Gly Arg Pro Met Trp

35 40 45

Leu Val Thr Arg Tyr Glu Asp Val Arg Lys Val Leu Thr Asp Pro Arg

50 55 60

Phe His Asn Asn Pro Ala Ser Val Pro Asp Ala Pro Asp Thr Arg Thr

65 70 75 80

Thr Ile Met Arg Gln Leu Asn Leu Pro Thr Asp Leu Val Asp Tyr Leu

85 90 95

Glu Asp Lys Leu Met Gln Ile Asp Gly Ala Asp His Arg Arg Leu Arg

100 105 110

Gly Leu Val Ser Lys Ala Phe Thr Ala Arg Arg Val Ala Arg Leu Arg

115 120 125

Pro Arg Val Glu Glu Ile Thr Thr Thr Leu Leu Asp Gly Met Ala Gln

130 135 140

Ala Gly Arg Asp Gly Thr Pro Val Asp Leu Leu Thr Ala Phe Cys Tyr

145 150 155 160

Pro Leu Pro Phe Thr Val Ile Cys Glu Leu Val Gly Ile Asp Glu Ala

165 170 175

Asp Arg Ala Pro Trp Phe Glu Trp Gly His Cys Leu Ser Ser Pro Thr

180 185 190

Glu His Arg Glu Arg Leu Pro Gln Val Met Arg Glu Cys Val Asp His

195 200 205

Met Leu Glu Ala Ile Glu Arg Arg Arg Ala Glu Pro Arg Asp Asp Leu

210 215 220

Ile Ser Ala Leu Val Gln Val Gln Glu Asp Asp Gly Asp Arg Leu Ser

225 230 235 240

Glu Arg Glu Met Val Thr Leu Val Phe Thr Leu Val Ile Thr Gly His

245 250 255

Glu Thr Thr Thr His Leu Leu Ala Asn Ser Val Leu Ala Leu Leu Glu

260 265 270

Asn Pro Asp Gln Leu Ala Leu Val Arg Ala Asp Pro Ser Arg Trp Pro

275 280 285

Gln Ala Val Asn Glu Leu Met Arg Leu Gly Pro Thr Gln Trp Gly Leu

290 295 300

Pro Arg His Pro Ser Glu Asp Val Glu Val Gly Gly Val Thr Ile Pro

305 310 315 320

Ala Gly Ala Thr Val Met Pro Leu Ile Leu Pro Ala Asn Thr Asp Pro

325 330 335

Arg Arg Tyr Asp Asp Pro Glu Arg Leu Asp Ile Gly Arg Glu Thr Gly

340 345 350

Gln Ser His Leu Gly Phe Gly Gln Gly Ala His Tyr Cys Leu Gly Ala

355 360 365

Pro Leu Ala Leu Gln Glu Ala Glu Val Ala Leu His Ala Leu Phe Glu

370 375 380

Arg Phe Pro Gly Leu Ser Leu Ala Val Pro Arg Glu Glu Val Val Trp

385 390 395 400

Thr Leu Arg Pro Gly Val Thr Arg Val Glu Lys Leu Pro Leu Arg Leu

405 410 415

Val

<210>37

<211>354

<212>PRT

<213>Streptomyces spectabilis

<400>37

Met Asn Ala Val Ala Thr Phe Thr Arg Leu Arg Glu Tyr Met Val Gly

1 5 10 15

Pro Ser Arg Phe Met Ile Leu Leu Ser Cys Phe Glu Leu Gly Ile Val

20 25 30

Asp Ala Leu Arg Lys Arg Pro Gly Leu Thr Ala Ala Gln Leu Gly Glu

35 40 45

Ala Val Ser Ala Lys Pro Asp Ala Val Glu Gln Leu Leu His Leu Pro

50 5560

Val Lys Glu Gly Phe Val Ala Phe Asp Lys Ala Ser Gly Gly Tyr Ser

65 70 75 80

Leu Asp Ala Phe Ala Gly Val Ser Asp Ala Asp Leu Gln Arg Val Leu

85 90 95

Pro Val Leu Asn Met Ile Lys Val Val Met Leu Arg Gln Leu Phe Tyr

100 105 110

Leu Thr Asp Ser Val Arg Thr Gly Ser Ile Val Gly Leu Asp Lys Leu

115 120 125

Tyr Gly Phe Glu Gly Asn Leu Tyr Glu Ala Val Ala Glu His Lys Asp

130 135 140

Leu Arg Glu Ser Trp Ala Thr Met Met Asp Asp Val Thr Ala His Met

145 150 155 160

Asp Pro Trp Phe Phe Arg Asn Ile His Ile Pro Pro Gly Ser Arg Val

165 170 175

Leu Asp Leu Ala Gly Asn Thr Gly Leu Gly Ala Ile Leu Thr His Arg

180 185 190

Leu Lys Ala Ser Pro Gly Leu Arg Val Thr Thr Phe Asp Leu Pro Glu

195 200 205

Lys Glu Ala Glu Cys Leu Glu Asn Phe Arg Ala Glu Gly Val Ala Glu

210 215 220

His Cys Ser Phe Ile Gly Gly Asp Val Phe Lys Ser Val Pro Lys Gly

225 230 235 240

Phe Asp Val Val Leu Ile Lys His Phe Leu Asp Met Phe Asp Lys Asp

245 250 255

Asp Val Arg Thr Ile Leu Asn Gly Val Ser Glu Ala Leu Asp Val Gly

260 265 270

Gly Lys Val Asn Ile Leu Val Pro Val Tyr Pro Glu Asp Ile Thr Glu

275 280 285

Pro Ser Ser Ala Gly Val Asp Ile Phe Pro Ala Tyr Phe Leu Ala Cys

290 295 300

Thr Met Gly Gln Gly Gly Pro Gln Lys Leu Ser Thr Tyr Arg Lys Trp

305 310 315 320

Leu Glu Glu Ala Gly Phe Arg Val Thr Arg Thr Val Ala Gln Asp Leu

325 330 335

Thr Thr Met Pro Gly Ala Leu Ile Ala His Gly Ile Leu Cys Ala Thr

340 345 350

Lys Val

<210>38

<211>266

<212>PRT

<213>Streptomyces spectabilis

<400>38

Met Thr Ala Val Asn Ala Trp Leu Ala Glu Asp Tyr Ala Gln Val Asn

1 5 10 15

Ala Pro His Ile Lys Thr Ala Leu Ala Ala Leu Glu Leu Ala Gly Gly

20 25 30

Ala Leu Glu Lys Gly Gly Ser Phe Ala Glu Ile Gly Cys Gly Thr Gly

35 40 45

Glu Val Ala Arg Ala Val Ala Glu Arg Gly Phe Asp Val Trp Ala Ser

50 55 60

Asp Ala Ser Pro Ser Met Val Glu Ala Thr Arg Gln Arg Cys Ala Gly

65 70 75 80

Leu Ser Val Arg Ala Asp Val Cys Pro Ala Glu Arg Leu Glu Leu Pro

85 90 95

Gln Gly Gln Phe Asp Leu Ile His Ser Ser Trp Val Leu His Trp Leu

100 105 110

Ala Glu Ala Glu Glu Pro Leu Arg Arg Met Ala Arg Ala Leu Arg Pro

115 120 125

Gly Gly His Leu Val Leu Gln Trp Ser Gly Ala Gln Pro Arg Gly Asp

130 135 140

Gly Ala Gly Met Phe Gly Ile Val Arg Glu Val Ser Gly Ser Ala Lys

145 150 155 160

Trp Arg Asp Leu LeuAla Ser Ala Pro Pro Ala Met Arg Asp Tyr Pro

165 170 175

Ala Asp Glu Val Ala Ala Val Leu Arg Asp Ala Gly Leu Glu Leu Val

180 185 190

Asp Tyr Asp Pro Ala Ile Val His Ser Leu Gly Gly Asn Gly Ser Leu

195 200 205

Pro Ser Asp Ala Glu Leu Ala Glu Met Arg Arg Arg Tyr Lys Leu Met

210 215 220

Gly Phe Ala Thr Gln Ala Asp Ala Leu Gly Glu Arg Val Asp Glu Phe

225 230 235 240

Val Asp Glu Val Leu Arg Ala Ala Phe Ala Ala Gly Lys Ala Gly Leu

245 250 255

His His Ala Arg Ile Val Ala Arg Arg Pro

260 265

<210>39

<211>64

<212>PRT

<213>Streptomyces spectabilis

<400>39

Met Lys Ile Ile Ala Asp Ala Asp Lys Cys Val Gly Ser Gly Gln Cys

1 5 10 15

Val Phe Thr Glu Pro Gly Leu Phe Asp Gln Ser Glu Asp Glu Gly Thr

20 25 30

Val Val Val Leu Asn Ala Glu Pro Glu Gly Glu Leu Leu Val Arg Arg

35 40 45

Ala His Glu Ala Val Asp Asn Cys Pro Gly Gln Ala Leu Ser Leu Ala

50 55 60

<210>40

<211>431

<212>PRT

<213>Streptomyces spectabilis

<400>40

Met Leu Asp Ser Pro Phe Arg Leu Gln Leu Pro Pro Ser Leu Tyr Val

1 5 10 15

Met Gly Ala Cys Glu Asn Ser Ser Cys Glu Thr Val Arg Leu Ala Thr

20 25 30

Leu Ser Phe Ser Ala Trp Ser Arg Gly Asp Leu Thr Glu Gly Leu Arg

35 40 45

Leu Ala Glu Arg Ala Gly Lys Thr Gln Pro Glu Cys Ser Gly Lys Cys

50 55 60

Val Gly Leu Ala Arg Ile Trp His Ser Ala Leu Leu Val Arg Ile Arg

65 70 75 80

Glu Pro Ala Ala Gly Ala Arg Val Leu Ala Gly Val Glu Glu Leu Thr

85 90 95

Ala Gly Thr Thr Thr Ser Gly Gly Leu Val Ala Ala Ser Leu Thr Cys

100 105 110

Ala Ala Glu Leu Ala Phe Thr Gln Gly Asp Ile Asp Thr Ala Val Asp

115 120 125

Leu Ala Glu Lys Gly Val Arg Glu Ala Gln Gln Leu Gly Leu Thr Cys

130 135 140

Leu Leu Pro Glu Ala His Ala Val Met Ala Leu Ser Ala Leu Arg Gln

145 150 155 160

Gly Asn Met Ser Val Ser Ser Asp Phe Ala Asn Gln Leu Arg Asp Asp

165 170 175

Ala Leu Leu Gly Gln Thr Ile His Arg Pro Ser Gln Cys Ala Trp Ala

180 185 190

Ala Ala Gln Leu Leu Glu Val Gln Gly Gly Ala Pro Ser Ala Ala His

195 200 205

Leu Val Val Gly Ile Ile Thr Asp Asp Gln Leu Ile Leu Glu Leu Leu

210 215 220

Ala Ala Gln Pro Ala Ala Ala Ala Trp Leu Val Arg Ala Ala Arg Lys

225 230 235 240

Leu Gly Asp Glu Val Leu Ala Lys Arg Val Ala Ala Glu Ala Ser Glu

245 250 255

Leu Ala Ala Arg Asn Arg Gly Ile Arg Ser Val Glu Ala Ala Gly Thr

260 265 270

His Ala Asp Gly Leu Val Ala Glu Asn Pro Glu Phe Leu Ile Ala Ala

275 280 285

Ala Glu Ala His Arg Asp Leu Trp Ala Gly Ala Ser Ala Tyr Glu Asp

290 295 300

Ala Gly Lys Leu Leu Ala Asp Thr Arg Gln Asp Arg Asp Arg Ala Val

305 310 315 320

Asp Val Leu Glu Arg Ala Val Asp Gly Tyr Ser Lys Ser Ser Ala Pro

325 330 335

Arg Asp Ala Arg Arg Val Val Ser Arg Leu Arg Ser Leu Gly Val Arg

340 345 350

Arg Gly Arg Tyr Pro Gln Tyr Ala Ala His Asp Ser Val Lys Val Gly

355 360 365

Ser Leu Thr Asn Ser Glu Val Ala Val Ala Glu Leu Val Ser Gln Gly

370 375 380

Phe Thr Asn Ser Gln Val Gly Glu Arg Leu Phe Ile Ser Gly His Thr

385 390 395 400

Val Ala Phe His Leu Lys Lys Ile Phe Arg Lys Met Asn Val Ala Ser

405 410 415

Arg Val Glu Leu Thr Arg Thr Trp Ser Arg Ile Ser Ser Ala Asp

420 425 430

<210>41

<211>119

<212>PRT

<213>Streptomyces spectabilis

<400>41

Met Lys His Asn Phe Lys Ala Ala Ala Gly Pro Gln Arg Cys Arg Leu

1 5 10 15

Arg Phe Asp Arg Phe Cys Ser Asp Leu Pro Gly Gly Ser Phe Pro Val

20 25 30

Ala Asn Ser Leu Leu Trp Pro Val Ser Tyr Leu Ser Arg Ser Ala Gly

35 40 45

Thr Tyr Gly Arg Val Thr Leu Ala Phe Ala Pro Ala Ser Ser Thr Pro

50 55 60

Ala Arg Arg Gly Ala Val Gly Ala Arg Gly Asp Arg Gly Leu Arg Gly

65 70 75 80

Leu Cys Arg Met Ile Leu Val Trp His Gly Gly Cys Leu Arg Asp His

85 90 95

Val Arg Val Cys Arg Pro Gly Gly Ser Gly Leu Val Gly Arg Ala Val

100 105 110

Arg Pro Gly Arg Ile Asp Gly

115

<210>42

<211>209

<212>PRT

<213>Streptomyces spectabilis

<400>42

Val Pro Thr Ser Thr Gln Ala Leu Glu Asn Phe Gly Asp Phe Asp Leu

1 5 10 15

Leu Leu Leu Asp Ser Ser Thr Pro Gly Met Asp Ser His Glu Val Cys

20 25 30

Lys Arg Ile Arg Asn Gln Ala Ser Val Pro Ile Ile Ile Leu Gly Pro

35 40 45

Cys Val Asn Glu Ile Asp Arg Val Leu Ala Leu Asn Ala Gly Ala Asp

50 55 60

Tyr Cys Val Val Lys Ser Asp Ser Leu His Glu Leu Gln Ala Ser Met

65 70 75 80

Asp Ala Val Met Arg Arg Ser Ser Pro Cys Pro Thr Gly Gln Asn Ala

85 90 95

Pro Gly Ser Ala Ala Gln Gly Gly Glu Ile Leu Val Ala Gly Pro Leu

100 105 110

Arg Leu Asn Pro Arg Asn Arg Asp Val Thr Leu Ala Gly Arg Pro Val

115 120 125

Arg Leu Thr Pro Lys Glu Phe Asp Leu Leu Ala Met Leu Met Glu Asp

130 135 140

Pro Asp Thr Leu His Thr Arg Lys Ala Ile Ile Ala Arg Val Trp Asn

145 150 155 160

Glu His Trp Phe Gly Ser Thr Arg Thr Leu Asp Val His Ile Gly Ala

165 170 175

Leu Arg Gln Lys Leu Gly Ser Tyr Glu Trp Ile Glu Thr Lys Arg Gly

180 185 190

Ile Gly Phe Arg Leu Gly Thr Val Pro Val Pro Gly Ala Arg Asp Ser

195 200 205

Pro

Claims (4)

1. A biosynthetic gene cluster of the antibiotic trexosin of Nagasa is characterized in that the nucleotide sequence of the gene cluster is shown as 1 st-95304 th in SEQ ID NO. 1; the related genes for coding the biosynthesis of the varicose chain hyphal bacteriocin, which are included in the gene cluster, are specifically as follows:

biosynthetic genes of the precursor 3-amino-5-hydroxybenzoic acid responsible for the biosynthesis of varicose streptothricin, i.e.stvG，stvH，stvI，stvJ，stvK，stvL，stvM，stvNA total of 8 genes:

stvGthe length of 1074 base pairs at the 76251-77324 base of SEQ ID NO.1, the length of coding 3-DHQ synthase is 357 amino acids, and the amino acid sequence is SEQ ID NO. 2;

stvHis positioned at the 77495-78649 th base in SEQ ID NO.1, has the length of 1155 base pairs, codes DAHP synthase, has the length of 384 amino acids and has the amino acid sequence of SEQ ID NO. 3;

stvIis located at the 78657-79469 th base in SEQ ID NO.1, has the length of 813 base pairs, encodes shikimate dehydrogenase, has the length of 270 amino acids and has the amino acid sequence of SEQ ID NO. 4;

stvKis located at the 79685-80845 base in the SEQ ID NO.1, has the length of 1161 base pairs, encodes AHBA synthase, has the length of 386 amino acids and has the amino acid sequence of SEQ ID NO. 5;

stvLthe coding oxidoreductase with the length of 271 amino acids is positioned at the base of 81127-81942 in the SEQ ID NO.1, and the coding oxidoreductase with the length of 271 amino acids has the amino acid sequence of SEQ ID NO. 6;

stvMthe nucleotide sequence is located at 82095-82679 of SEQ ID NO.1The gene has the length of 585 base pairs, encodes phosphoglycolic acid phosphatase, has the length of 194 amino acids, and has the amino acid sequence of SEQ ID NO. 7;

stvNpositioned at the site of 82699-83652 base in SEQ ID NO.1, the length of 954 base pairs, the coding gene is glucokinase, the length of 317 amino acids, and the amino acid sequence is SEQ ID NO. 8;

stvJlocated at 94975-94424 base in SEQ ID NO.1, the length is 552 base pairs, the length is 183 amino acids, the amino acid sequence is SEQ ID NO. 9;

the gene responsible for the extension of the carbon skeleton of streptothricin, i.e.stvA，stvB，stvC，stvD，stvEA total of 5 genes:

stvAis positioned at the 20415-37088 th base of the SEQ ID NO.1, has the length of 16674 base pairs, encodes polyketide synthase, has the length of 5557 amino acids and has the amino acid sequence of SEQ ID NO. 10;

stvBlocated at the 37120-47883 base pairs in SEQ ID NO.1, has the length of 10764 base pairs, encodes polyketide synthase, has the length of 3587 amino acids and has the amino acid sequence of SEQ ID NO. 11;

stvClocated at the 48086-fold 58798 th base in SEQ ID NO.1, 10713 base pairs in length, encodes polyketide synthase, 3570 amino acids in length, and has the amino acid sequence of SEQ ID NO. 12;

stvDlocated at the base 58958-64522 of SEQ ID NO.1, has a length of 5565 base pairs, encodes polyketide synthase, has a length of 1854 amino acids, and has the amino acid sequence of SEQ ID NO. 13;

stvEthe length of the coding sequence is 10713 base pairs at the 64592-75304 th base in SEQ ID NO.1, the coding sequence encodes polyketide synthase, the length of the coding sequence is 3570 amino acids, and the amino acid sequence is SEQ ID NO. 14;

genes responsible for post-modification in the biosynthesis of varicose streptothricinstvA1，orf1309，orf1308，orf1307，orf1306，orf1305，orf1304，orf1303，orf1302，orf1301，stvO1，stvO2，stvO3，stvP1，stvM1，stvP2，stvA2，stvP3，stvF，stvP4，stvO4，stvP5，stvM2，stvM3，orf1272，stvR1，orf1270，stvR2A total of 28 genes:

stvA1located at the 348-707 th base in the SEQ ID NO.1, the length is 360 base pairs, the length codes acyltransferase, the length is 119 amino acids, and the amino acid sequence is SEQ ID NO. 15;

orf1309is positioned at the 740-st and 1354-th bases in the SEQ ID NO.1, has the length of 615 base pairs, encodes aryl sulfatase, has the length of 204 amino acids and has the amino acid sequence of SEQ ID NO. 16;

orf1308is positioned at the 1380-1703 th base in the SEQ ID NO.1, has the length of 324 base pairs, encodes unknown protein, has the length of 107 amino acids and has the amino acid sequence of SEQ ID NO. 17;

orf13071440 bp in length located at the 3161-1722 nd base of SEQ ID NO.1, encodes 6-phosphogluconate dehydrogenase with 479 amino acids in length and has the amino acid sequence of SEQ ID NO. 18;

orf1306located at the 3335-4306 th base in SEQ ID NO.1, the length is 972 base pairs, the acetyl xylan esterase is coded, the length is 323 amino acids, and the amino acid sequence is SEQ ID NO. 19;

orf1305at the 4428-5765 th base in SEQ ID NO.1, the length of 1338 base pairs, the length of coding peptidase, the length of 445 amino acids, the amino acid sequence of SEQ ID NO. 20;

orf1304is positioned at the 7074-5854 th base in the SEQ ID NO.1, has the length of 1221 base pairs, encodes a membrane protein, has the length of 406 amino acids and has the amino acid sequence of SEQ ID NO. 21;

orf1303located at the 7347-8063 site base of SEQ ID NO.1, the length is 717 base pairs, the length encodes trivalent iron reductase, the length is 238 amino acids, and the amino acid sequence is SEQ ID NO. 22;

orf1302located at the 8239-9288 th base in SEQ ID NO.1, is 1050 base pairs in length, encodes a glycosyltransferase, and has a length of349 amino acids, and the amino acid sequence is SEQ ID NO. 23;

orf1301is positioned at the position of the 9379-10599 th base in the SEQ ID NO.1, has the length of 1221 base pairs, codes for the glucosyl-1-phosphoadenylyl transferase, has the length of 406 amino acids and has the amino acid sequence of SEQ ID NO. 24;

stvO1the length of the amino acid sequence is 969 base pairs at the 10846-11814 th base in SEQ ID NO.1, the length of the amino acid sequence is 322 amino acids, and the amino acid sequence is SEQ ID NO. 25;

stvO2is positioned at the 11823-th and 12806-th bases in SEQ ID NO.1, has the length of 984 base pairs, encodes oxidoreductase, has the length of 327 amino acids and has the amino acid sequence of SEQ ID NO. 26;

stvO3is positioned at the 12889-13452 th base in the SEQ ID NO.1, has the length of 564 base pairs, encodes NADPH-dependent reductase, has the length of 187 amino acids and has the amino acid sequence of SEQ ID NO. 27;

stvP1is positioned at the 13481-14773 th base in the SEQ ID NO.1, has the length of 1293 base pairs, codes cytochrome P450, has the length of 430 amino acids and has the amino acid sequence of SEQ ID NO. 28;

stvM1located at the 15159-15767 th base in SEQ ID NO.1, has the length of 609 base pairs, encodes methyltransferase, has the length of 202 amino acids and has the amino acid sequence of SEQ ID NO. 29;

stvP2is positioned at the position of the 15800-17005 th base in the SEQ ID NO.1, has the length of 1206 base pairs, codes cytochrome P450, has the length of 401 amino acids and has the amino acid sequence of SEQ ID NO. 30;

stvA2the base is positioned at 18275-17106 th base in SEQ ID NO.1, the length is 1170 base pairs, the fatty acyl transferase is coded, the length is 389 amino acids, and the amino acid sequence is SEQ ID NO. 31;

stvP3positioned at the base site 18765-19958 in the SEQ ID NO.1, has the length of 1194 base pairs, encodes cytochrome P450, has the length of 397 amino acids, and has the amino acid sequence of SEQ ID NO. 32;

stvFbase at position 75408-76202 in SEQ ID NO.1The length of the base is 795 base pairs, the length of the base is 264 amino acids, and the amino acid sequence is SEQ ID NO. 33;

stvP4is positioned at the base 83977-85221 in the SEQ ID NO.1, has the length of 1245 base pairs, codes cytochrome P450, has the length of 414 amino acids and has the amino acid sequence of SEQ ID NO. 34;

stvO4is positioned at the 86934-85273 site base in the SEQ ID NO.1, is 1662 base pairs in length, encodes propionate hydroxylase, is 553 amino acids in length, and has an amino acid sequence of SEQ ID NO. 35;

stvP5positioned at the base 87228-88481 in the SEQ ID NO.1, has the length of 1254 base pairs, encodes cytochrome P450, has the length of 417 amino acids and has the amino acid sequence of SEQ ID NO. 36;

stvM2positioned at the 89660-88593 th base in the SEQ ID NO.1, the length is 1068 base pairs, the methyl transferase is coded, the length is 355 amino acids, and the amino acid sequence is SEQ ID NO. 37;

stvM3is located at the base positions 90540-89737 in the SEQ ID NO.1, has the length of 804 base pairs, codes for methyltransferase with the length of 267 amino acids and has the amino acid sequence SEQ ID NO. 38;

orf1272is positioned at the base positions 90982-90785 in the SEQ ID NO.1, has the length of 198 base pairs, encodes the ferroxyl enzyme, has the length of 65 amino acids, and has the amino acid sequence of SEQ ID NO. 39;

stvR1is positioned at the 92612 + 91314 site base in SEQ ID NO.1, has the length of 1299 base pairs, encodes a regulatory factor with the length of 432 amino acids and has the amino acid sequence of SEQ ID NO. 40;

orf1270the length of the gene is 360 base pairs at the 92941- -;

stvR2is located at bases 93785-94414 in SEQ ID NO.1, has the length of 630 base pairs, encodes a regulatory factor, has the length of 209 amino acids and has the amino acid sequence SEQ ID NO. 42.

2. A recombinant vector, expression cassette, transgenic cell line or recombinant bacterium comprising the biosynthetic gene cluster of varicose streptothricin according to claim 1.

3. Use of the recombinant vector, expression cassette, transgenic cell line or recombinant bacterium of claim 2 for the synthesis of streptothricin.

4. A method for synthesizing varicose streptothricin, which is to ferment the recombinant bacteria of claim 2, collect and extract fermentation products, and obtain the compounds of the varicose streptothricin series.

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