CN107522850A - A kind of preparation method of water-soluble curcumin polymer - Google Patents
A kind of preparation method of water-soluble curcumin polymer Download PDFInfo
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Abstract
The present invention relates to medication medication to synthesize field, more particularly to a kind of preparation method of water-soluble curcumin polymer；Comprise the following steps：(1) batch mixing, (2) acidifying, the preparation of (3) polymer；The inventive method is simple, it is easy to operate, the curcumin polymer being prepared has outstanding heat endurance, storage stability, controllable molecular weight and controllable degradation rate, it is obtained that there is excellent water miscible curcumin polymer, it can operate with anti-oxidant, it is antiviral, liver protection, cholagogic, anticancer, reducing blood lipid, anti AIDS virus, it is antibacterial etc., with curcumin in itself compared with, not only drastically increase the stability and bioavailability of curcumin, significantly improve cytotoxicity and anti-cancer ability of the curcumin to cancer cell simultaneously, it is expected to turn into a kind of new cancer therapy drug.
Description
Technical field
The present invention relates to pharmaceutical synthesis field, more particularly to a kind of preparation method of water-soluble curcumin polymer.
Background technology
Curcumin is a kind of natural, structure novelty, with multiple biological activities Polyphenols natural products, and its is basic
Skeleton is respectively connected an aromatic ring and formed by middle by the carbochain and carbochain both ends of seven carbon atoms, wherein aromatic ring 4-OH and 3-
OCH3 structure is that it has the active group of strong oxidation resistance.Curcumin is mainly isolated from ginger,
It is the main component that Turmeric plays pharmacological activity, it has antibacterial, anti-oxidant, antiviral, liver protection, cholagogic, anticancer, drop blood
Fat, anti AIDS virus etc. act on.It has been used widely in food coloring, food additives and field of medicaments at present.In recent years,
Once it was considered as a kind of reason because curcumin is the natural products that a kind of toxic side effect is small, molecular weight is small, less toxic, cheap and easy to get
The cancer therapy drug thought, also as the focus of domestic and foreign scholars research.With research of the people to curcumin and its derivative
Progressively deeply, the bioactivity and pharmacological activity of curcumin gradually by it is found that and accreditation, national cancer institute is
Through having carried out Ι clinical trial phases using turmeric.Comprehensive and in-depth study is continued to curcumin active anticancer, makes it be expected to be opened
Send out into a kind of new, less toxic, efficient cancer therapy drug.
In recent years because curcumin is antitumor, immunological regulation, anti-oxidant, antiatherosclerosis effect, even more cause state
The concern of inside and outside scholar, curcumin also show huge development potentiality.However, due to curcumin poorly water-soluble, be not easy by
Body absorption, it is metabolized again quickly, so that its bioavilability is very low.In addition, curcumin under neutral or basic conditions can
Fast decoupled, and water can not be dissolved in acid condition, this causes it from being used directly as medicine again.In order to improve
The bioavilability of curcumin, and do not influence its bioactivity, scholar HuadongTang etc. on the basis of certain and utilize turmeric
The polycondensation reaction of plain phenolic hydroxyl group and different monomers has synthesized eight curcumin polymers, wherein IV -1 pair of tumour cell of polymer
SKOV-3 shows the bioactivity of highly significant；Scholar YouqingShen etc. is disclosed in US20120003177A1 patent
A kind of preparation method of curcumin polymer, and using this kind of method connected on curcumin hydrophilic radical PEG synthesized it is several
Individual curcumin polymer, improve the water solubility of curcumin.Scholar YifengZhu etc. has synthesized a kind of new molecular weight as 6.1
× 104Da the curcumin polymer IV -2 containing selenium, experiment show compound stabilization, good water solubility, the injectable, and to mouse
There is good effect of anti hepatic fibrosis.Scholar NoriyoshiMATSUMI etc. using curcumin respectively with two other monomer last of the ten Heavenly stems two
Acyl chlorides, suberoyl chlorine polycondensation reaction have synthesized the polymer IV -3, IV -4 of different molecular weight, and recycle in obtained polymer
Two carbonyls and metal Ru of curcumin coordinate the metal coordinating polymer for having obtained HMW, and new work(is developed to hope
Energy.
Understood from document above report, it is biological although curcumin poorly water-soluble, being not easy by body absorption, metabolism again quickly
Availability is very low, but curcumin has again as a kind of natural products cheap and easy to get, toxic side effect is small, molecular weight is small, less toxic
There are for example anti-oxidant multiple biological activities, antiviral, liver protection, cholagogic, anticancer, reducing blood lipid, anti AIDS virus, antibacterial etc..In view of ginger
The fabulous bioactivity of flavine and pharmacological activity, asked to improve the dissolubility of curcumin and its drugloading rate as medicine
Topic, design synthesis curcumin macromolecule polyalcohol have important economic implications and realistic meaning, had to medical development positive
Facilitation.
The content of the invention
The present invention is in order to solve the above technical problems, provide a kind of preparation method of water-soluble curcumin polymer.
Realized particular by following technical scheme：
A kind of preparation method of water-soluble curcumin polymer, comprises the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:1~5 mass ratio mixing,
First catalyst 1 is added in 100ml three mouthfuls of round-bottomed bottles, then the mixture is added to three mouthfuls of round-bottomed bottles, is mixed thoroughly, then is added
Enter the 2-6% times of triethylamine measured of curcumin quality, 1~4h of stirring reaction under normal temperature, obtain material 1；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2~6, and stirring in water bath filters, by filter cake to yellow solid is separated out
It is washed with water to neutrality, dry crude product, is recrystallized crude product with chloroform, dries faint yellow solid of weighing to obtain, be calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, N, N'- bis- are sequentially added
Carbodicyclo hexylimide, 4-N, N'- dimethylamino naphthyridines, expect 2 and poly glycol monomethyl ether, 3h is stirred under normal temperature, filter, filtrate
The sodium carbonate liquor for being 20%-60% with content washs 3~4 times, then is washed till neutrality with saturated nacl aqueous solution, through anhydrous slufuric acid
After magnesium is dried, solvent is boiled off, produces Inventive polymers.
A kind of preparation method of water-soluble curcumin polymer, comprises the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:1~5 mass ratio mixing,
First catalyst 1 is added in 100ml three mouthfuls of round-bottomed bottles, then the mixture is added to three mouthfuls of round-bottomed bottles, is mixed thoroughly, then is added
Enter triethylamine, 1~4h of stirring reaction under normal temperature, obtain material 1；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2-6, and stirring in water bath filters to yellow solid is separated out, filter cake is used
It is washed to neutrality, dry crude product, crude product is recrystallized with chloroform, faint yellow solid of weighing to obtain is dried, is calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, sequentially add dichloromethane, triethylamine,
Three phosgenes, material 2 and poly glycol monomethyl ether, the stirring reaction 1-4h under ice-water bath (0 DEG C) are filtered, and filtrate is with content
20%-60% sodium carbonate liquor washs 3~4 times, then is washed till neutrality with saturated nacl aqueous solution, is dried through anhydrous magnesium sulfate
Afterwards, solvent is boiled off, produces Inventive polymers.
Described curcumin after purification, it is to soak curcumin in ethanol, stirs 15~25min, filtration drying is
Can；Described pyromellitic acid anhydride after purification, is that pyromellitic acid anhydride is immersed in solution of acetic anhydride, stirring 15~
25min, filtration drying.
Catalyst 1 in described step (2), is one kind in tetrahydrofuran and dichloromethane, wherein catalyst 1 and ginger
The accounting of flavine is 10ml：1~15mg.
Dichloromethane in described step (3), itself and expect 2 adding proportion be 10ml：1~15mg.
Described N, N'- dicyclohexylcarbodiimide, addition are expect 2 mass 3~10%.
Described 4-N, N'- dimethylamino naphthyridine, addition are the 0.2%-0.6% for expecting 2 mass.
Described triethylamine, addition are the 2-6% for expecting 2 mass.
Three described phosgenes, addition are the 1-5% for expecting 2 mass.
Described material 2 and poly glycol monomethyl ether, mass ratio 1:1~5.
In summary, the beneficial effects of the present invention are：The inventive method is simple, easy to operate, the turmeric being prepared
Plain polymer has outstanding heat endurance, storage stability, controllable molecular weight and controllable degradation rate, obtained tool
There is excellent water miscible curcumin polymer, can operate with anti-oxidant, antiviral, liver protection, cholagogic, anticancer, reducing blood lipid, anti-AIDS
It is viral, antibacterial etc., with curcumin in itself compared with, not only drastically increase the stability and bioavailability of curcumin, simultaneously
Cytotoxicity and anti-cancer ability of the curcumin to cancer cell are significantly improved, is expected to turn into a kind of new cancer therapy drug.
Brief description of the drawings
Fig. 1 is cytotoxic effect figure of the curcumin polymer to cancer cell of the preparation of embodiment 1.
Embodiment
The embodiment of the present invention is described in further detail below, but the invention is not limited in these realities
Mode is applied, any improvement or replacement on the present embodiment essence spirit, it is claimed to still fall within the claims in the present invention
Scope.
Embodiment 1
A kind of preparation method of water-soluble curcumin polymer, comprises the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:1 mass ratio mixing, first
Tetrahydrofuran is added in 100ml three mouthfuls of round-bottomed bottles, the accounting of tetrahydrofuran and curcumin is 10ml：1mg, then by described in
Mixture is added to three mouthfuls of round-bottomed bottles, mixes thoroughly, adds the triethylamine of 2% times of curcumin quality amount, stirring reaction 1 under normal temperature
~4h, obtain material 1；Described curcumin after purification, it is to soak curcumin in ethanol, stirs 15min, filtration drying is
Can；Described pyromellitic acid anhydride after purification, is that pyromellitic acid anhydride is immersed in solution of acetic anhydride, stirring
15min, filtration drying；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2~6, and stirring in water bath filters, by filter cake to yellow solid is separated out
It is washed with water to neutrality, dry crude product, is recrystallized crude product with chloroform, dries faint yellow solid of weighing to obtain, be calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, 2 mass of material are sequentially added
3% times of amount N, N'- dicyclohexylcarbodiimides, the 4-N for expecting 0.2%% times of 2 mass amount, N'- dimethylamino naphthyridines, material
2nd, expect the poly glycol monomethyl ether of 2 mass, 1 times of amount, 3h is stirred under normal temperature, filter, the sodium carbonate that filtrate content is 20%% is molten
Liquid washs 3~4 times, then is washed till neutrality with saturated nacl aqueous solution, after anhydrous magnesium sulfate is dried, boils off solvent, produces this hair
The adding proportion of bright polymer, wherein dichloromethane and material 2 is 10ml：1mg.
Embodiment 2
A kind of preparation method of water-soluble curcumin polymer, comprises the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:5 mass ratio mixing, first
Dichloromethane is added in 100ml three mouthfuls of round-bottomed bottles, wherein the accounting of dichloromethane and curcumin is 10ml：15mg, then
The mixture is added to three mouthfuls of round-bottomed bottles, mixed thoroughly, the triethylamine of 6% times of amount of curcumin quality is added, is stirred under normal temperature
1~4h of reaction is mixed, obtains material 1；Described curcumin after purification, it is to soak curcumin in ethanol, stirs 25min, filtering
Drying；Described pyromellitic acid anhydride after purification, is that pyromellitic acid anhydride is immersed in solution of acetic anhydride, stirs
Mix 25min, filtration drying；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2~6, and stirring in water bath filters, by filter cake to yellow solid is separated out
It is washed with water to neutrality, dry crude product, is recrystallized crude product with chloroform, dries faint yellow solid of weighing to obtain, be calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, 2 mass of material are sequentially added
10% times of amount N, N'- dicyclohexylcarbodiimides, the 4-N for expecting 0.6% times of 2 mass amount, N'- dimethylamino naphthyridines, material
2nd, expect the poly glycol monomethyl ether of 2 mass, 5 times of amounts, 3h is stirred under normal temperature, filter, filtrate content is 60% sodium carbonate liquor
Washing 3~4 times, then neutrality is washed till with saturated nacl aqueous solution, after anhydrous magnesium sulfate is dried, solvent is boiled off, produces the present invention
The adding proportion of polymer, wherein dichloromethane and material 2 is 10ml：15mg.
Embodiment 3
A kind of preparation method of water-soluble curcumin polymer, comprises the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:3 mass ratio mixing, first
Tetrahydrofuran is added in 100ml three mouthfuls of round-bottomed bottles, the accounting of tetrahydrofuran and curcumin is 10ml：8mg, then by described in
Mixture is added to three mouthfuls of round-bottomed bottles, mixes thoroughly, adds the triethylamine of 4% times of curcumin quality amount, stirring reaction 1 under normal temperature
~4h, obtain material 1；Described curcumin after purification, it is to soak curcumin in ethanol, stirs 20min, filtration drying is
Can；Described pyromellitic acid anhydride after purification, is that pyromellitic acid anhydride is immersed in solution of acetic anhydride, stirring
20min, filtration drying；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2~6, and stirring in water bath filters, by filter cake to yellow solid is separated out
It is washed with water to neutrality, dry crude product, is recrystallized crude product with chloroform, dries faint yellow solid of weighing to obtain, be calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, 2 mass of material are sequentially added
6% times of amount N, N'- dicyclohexylcarbodiimides, the 4-N for expecting 0.4% times of 2 mass amount, N'- dimethylamino naphthyridines, material 2,
Expect the poly glycol monomethyl ether of 2 mass, 3 times of amounts, 3h is stirred under normal temperature, filter, filtrate is washed with the sodium carbonate liquor that content is 40%
Wash 3~4 times, then neutrality is washed till with saturated nacl aqueous solution, after anhydrous magnesium sulfate is dried, boil off solvent, it is poly- to produce the present invention
The adding proportion of compound, wherein dichloromethane and material 2 is 10ml：8mg.
Embodiment 4
A kind of preparation method of water-soluble curcumin polymer, comprises the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:1 mass ratio mixing, first
Tetrahydrofuran is added in 100ml three mouthfuls of round-bottomed bottles, wherein the accounting of tetrahydrofuran and curcumin is 10ml：15mg, then
The mixture is added to three mouthfuls of round-bottomed bottles, mixed thoroughly, adds triethylamine, 1~4h of stirring reaction under normal temperature, obtains material 1；It is described
Curcumin after purification, be by curcumin immersion in ethanol, stir 15min, filtration drying；It is described after purification
Pyromellitic acid anhydride, it is that pyromellitic acid anhydride is immersed in solution of acetic anhydride, stirs 25min, filtration drying；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2-6, and stirring in water bath filters to yellow solid is separated out, filter cake is used
It is washed to neutrality, dry crude product, crude product is recrystallized with chloroform, faint yellow solid of weighing to obtain is dried, is calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, 2 mass of material are sequentially added
2% times of amount triethylamine, expect three phosgenes of 5% times of 2 mass amount, material 2, expect 2 mass 1 times of amount polyethyleneglycol first
Ether, the stirring reaction 1-4h under 0 DEG C of water-bath, filter, filtrate is washed 3~4 times with the sodium carbonate liquor that content is 20%%, then is used
Saturated nacl aqueous solution is washed till neutrality, after anhydrous magnesium sulfate is dried, boils off solvent, produces Inventive polymers, wherein dichloro
The adding proportion of methane and material 2 is 10ml：15mg.
Embodiment 5
A kind of preparation method of water-soluble curcumin polymer, comprises the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:5 mass ratio mixing, first
Dichloromethane is added in 100ml three mouthfuls of round-bottomed bottles, wherein the accounting of dichloromethane and curcumin is 10ml：1mg, then will
The mixture is added to three mouthfuls of round-bottomed bottles, mixes thoroughly, adds triethylamine, 1~4h of stirring reaction under normal temperature, obtains material 1；Described
Curcumin after purification, it is to soak curcumin in ethanol, stirs 15min, filtration drying；Described after purification equal
PMDA, it is that pyromellitic acid anhydride is immersed in solution of acetic anhydride, stirs 25min, filtration drying；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2-6, and stirring in water bath filters to yellow solid is separated out, filter cake is used
It is washed to neutrality, dry crude product, crude product is recrystallized with chloroform, faint yellow solid of weighing to obtain is dried, is calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, 2 mass of material are sequentially added
6% times of amount triethylamine, expect three phosgenes of 1% times of 2 mass amount, material 2, expect 2 mass 5 times of amounts polyethyleneglycol first
Ether, the stirring reaction 1-4h under 0 DEG C of water-bath, filter, filtrate with content be 60% sodium carbonate liquor wash 3~4 times, then with satisfy
Neutrality is washed till with sodium chloride solution, after anhydrous magnesium sulfate is dried, boils off solvent, produces Inventive polymers, wherein dichloromethane
The adding proportion of alkane and material 2 is 10ml：1mg.
Embodiment 6
A kind of preparation method of water-soluble curcumin polymer, comprises the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:2 mass ratio mixing, first
Tetrahydrofuran is added in 100ml three mouthfuls of round-bottomed bottles, wherein the accounting of tetrahydrofuran and curcumin is 10ml：10mg, then
The mixture is added to three mouthfuls of round-bottomed bottles, mixed thoroughly, adds triethylamine, 1~4h of stirring reaction under normal temperature, obtains material 1；It is described
Curcumin after purification, be by curcumin immersion in ethanol, stir 20min, filtration drying；It is described after purification
Pyromellitic acid anhydride, it is that pyromellitic acid anhydride is immersed in solution of acetic anhydride, stirs 15min, filtration drying；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2-6, and stirring in water bath filters to yellow solid is separated out, filter cake is used
It is washed to neutrality, dry crude product, crude product is recrystallized with chloroform, faint yellow solid of weighing to obtain is dried, is calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, 2 mass of material are sequentially added
5% times of amount triethylamine, expect three phosgenes of 4% times of 2 mass amount, material 2, expect 2 mass 3 times of amounts polyethyleneglycol first
Ether, the stirring reaction 1-4h under 0 DEG C of water-bath, filter, filtrate with content be 50% sodium carbonate liquor wash 3~4 times, then with satisfy
Neutrality is washed till with sodium chloride solution, after anhydrous magnesium sulfate is dried, boils off solvent, produces Inventive polymers, wherein dichloromethane
The adding proportion of alkane and curcumin is 10ml：5mg.
By taking curcumin polymer (abbreviation polymer 1) prepared by embodiment 1 as an example, detection cell generally in the art is utilized
MTT (3- (4,5- dimethylthiazole -2) -2,5- diphenyltetrazolium bromide bromides) method of survival and growth detection polymer 1 is thin to cancer
Born of the same parents SKOV-3, OVCAR, MCF-7 cytotoxicity, SKOV-3 cells, OVCAR cells, MCF-7 cells are used into polymer 1 respectively
72h is handled, 24h is then cultivated in blank cultures, calculates the survival rate of cancer cell, it is specific as shown in figure 1, wherein, 1 represents
Polymer 1 acts on SKOV-3；2 expression polymer 1 act on OVCAR；3 expression polymer 1 act on MCF-7；4 represent turmeric
Element acts on SKOV-3.
Wherein, the Cleaning Principle of mtt assay is：Succinate dehydrogenase in living cells mitochondria can reduce exogenous MTT
Bluish violet for water-insoluble crystallizes first a ceremonial jade-ladle, used in libation (Formazan) and is deposited in cell, and dead cell is without this function.Dimethyl is sub-
Sulfone (DMSO) can dissolve the first a ceremonial jade-ladle, used in libation in cell, and its absorbance value is determined at 490nm wavelength with enzyme-linked immunosorbent assay instrument, can be indirect
Reflect living cells quantity, in the range of certain cell number, it is directly proportional to cell number that MTT crystallizes the amount to be formed.
As can be seen from Figure 1：Curcumin polymer prepared by embodiment 1 has than curcumin to SKOV-3, OVCAR, MCF-7
Higher cytotoxicity, by the way that curcumin is made into polymer, action effect of the curcumin to cancer cell is drastically increased, is had
Clinical practice is realized in prestige.
In the mouse experiment in vivo exemplified by the polymer synthesized by embodiment 1, the dosage for injecting medicine is 100mg/kg,
After 48 hours, the tumor weight of the mouse of curcumin polymer prepared by injection embodiment 1 is 0.3523g, and the injection compareed
Tumour in the Mice Body of phosphate buffer (PBS) is 1.2532g, and this proves curcumin polymer tool prepared by embodiment 1
There is the ability of high suppression tumour growth.
Claims (10)
1. a kind of preparation method of water-soluble curcumin polymer, it is characterised in that comprise the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:1~5 mass ratio mixing, first will
Catalyst 1 is added in 100ml three mouthfuls of round-bottomed bottles, then the mixture is added into three mouthfuls of round-bottomed bottles, is mixed thoroughly, is added ginger
The triethylamine that 2-6% times of flavine quality is measured, 1~4h of stirring reaction under normal temperature, obtains material 1；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2~6, and stirring in water bath filters, by filter cake water to yellow solid is separated out
It is washed till neutrality, dry crude product, crude product is recrystallized with chloroform, faint yellow solid of weighing to obtain is dried, is calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, N, the hexamethylenes of N'- bis- are sequentially added
Base carbodiimide, 4-N, N'- dimethylamino naphthyridines, material 2 and poly glycol monomethyl ether, 3h being stirred under normal temperature, is filtered, filtrate is with containing
Measure and washed 3~4 times for 20%-60% sodium carbonate liquor, then neutrality is washed till with saturated nacl aqueous solution, done through anhydrous magnesium sulfate
After dry, solvent is boiled off, produces Inventive polymers.
2. a kind of preparation method of water-soluble curcumin polymer, it is characterised in that comprise the following steps：
(1) batch mixing：Curcumin after purification and pyromellitic acid anhydride after purification are pressed 1:1~5 mass ratio mixing, first will
Catalyst 1 is added in 100ml three mouthfuls of round-bottomed bottles, then the mixture is added into three mouthfuls of round-bottomed bottles, is mixed thoroughly, is added three
Ethamine, 1~4h of stirring reaction under normal temperature, obtains material 1；
(2) it is acidified：PH value with hydrochloric acid regulation material 1 is 2-6, and stirring in water bath filters to yellow solid is separated out, filter cake is washed with water
To neutrality, dry crude product, crude product is recrystallized with chloroform, dry faint yellow solid of weighing to obtain, be calculated as material 2；
(3) preparation of polymer：In dry 100ml three neck round bottom flask, dichloromethane, triethylamine, three carbon are sequentially added
Acyl chlorides, material 2 and poly glycol monomethyl ether, the stirring reaction 1-4h under ice-water bath (0 DEG C), are filtered, filtrate is 20%- with content
60% sodium carbonate liquor washs 3~4 times, then is washed till neutrality with saturated nacl aqueous solution, after anhydrous magnesium sulfate is dried, boils off
Solvent, produce Inventive polymers.
3. the preparation method of water-soluble curcumin polymer as claimed in claim 1 or 2, it is characterised in that described purifying
Curcumin afterwards, it is to soak curcumin in ethanol, stirs 15~25min, filtration drying；Described after purification equal
PMDA, it is that pyromellitic acid anhydride is immersed in solution of acetic anhydride, stirs 15~25min, filtration drying.
4. the preparation method of water-soluble curcumin polymer as claimed in claim 1 or 2, it is characterised in that described step
(2) catalyst 1 in, it is one kind in tetrahydrofuran and dichloromethane, the accounting of wherein catalyst 1 and curcumin is 10ml：1
~15mg.
5. the preparation method of water-soluble curcumin polymer as claimed in claim 1 or 2, it is characterised in that described step
(3) dichloromethane in, itself and expect 2 adding proportion be 10ml：1~15mg.
6. the preparation method of water-soluble curcumin polymer as claimed in claim 1, it is characterised in that described N, N'- bis-
Carbodicyclo hexylimide, addition are expect 2 mass 3~10%.
7. the preparation method of water-soluble curcumin polymer as claimed in claim 1, it is characterised in that described 4-N, N'-
Dimethylamino naphthyridine, addition are the 0.2%-0.6% for expecting 2 mass.
8. the preparation method of water-soluble curcumin polymer as claimed in claim 1, it is characterised in that described triethylamine,
Addition is the 2-6% for expecting 2 mass.
9. the preparation method of water-soluble curcumin polymer as claimed in claim 1, it is characterised in that three described phosphinylidynes
Chlorine, addition are the 1-5% for expecting 2 mass.
10. the preparation method of water-soluble curcumin polymer as claimed in claim 1, it is characterised in that described material 2 and poly-
Glycol monoethyl ether, mass ratio 1:1~5.
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| CN101628969A (en) * | 2009-08-03 | 2010-01-20 | 浙江大学 | Method for preparing curcumin polymer |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108653721A (en) * | 2018-05-28 | 2018-10-16 | 刘河 | The chitin carrier drug and preparation method and purposes of water-soluble curcumin derivate |
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