CN107517955B - Composite rice seed life prolonging agent and preparation method and application thereof - Google Patents
Composite rice seed life prolonging agent and preparation method and application thereof Download PDFInfo
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Abstract
The invention belongs to the field of rice seeds, and discloses a compound rice seed life prolonging agent, and a preparation method and application thereof. The compound rice seed life prolonging agent is prepared from the following raw materials in percentage by mass: 21.00-27.00% of vine tea extract, 17.00-25.00% of bitter almond extract, 5.00-10.00% of clove oil extract, 10.00-15.00% of tea tree essential oil extract, 17.50-22.50% of coumarin extract, 5.00-10.00% of paclobutrazol and 5.00-10.00% of agricultural streptomycin 72% wettable powder. Weighing all the raw materials according to the proportion, placing the raw materials into a blending tank, uniformly stirring, and packaging or canning the raw materials in a composite aluminum bag to obtain the composite rice seed life extender. The invention has reasonable formula, simple processing and preparation and convenient use, and provides an ideal medicament for prolonging the service life of rice seeds.
Description
Technical Field
The invention belongs to the field of rice seeds, and particularly relates to a compound rice seed life prolonging agent as well as a preparation method and application thereof.
Background
Seed longevity refers to the average age at which a seed can retain its viability under certain storage conditions, i.e., the time it takes for a seed to go from full maturity to loss of viability. In actual production, seed longevity is a group concept, which refers to the period of time that a batch of seeds passes from harvest to when germination rate decreases to 50%, also called the half-life of the seeds. Under the common conditions, the seeds of the rice are deteriorated during storage, the germination rate is reduced, and particularly, the germination rate of the hybrid rice seeds can be reduced to below 70 percent after one year of storage, so that the hybrid rice seeds cannot meet the production requirement and are discarded, thereby causing huge loss. The technical requirements for prolonging the service life of the seeds are more urgent for the preservation of the national strategic barren seeds and the long-term storage of germplasm resources.
The longevity of seeds is determined by genetic factors, environmental conditions during seed development, and storage conditions, i.e., by the quality of the seeds, storage temperature, and moisture content of the seeds. Research shows that the life of the seeds is shortened in the storage process mainly because of the comprehensive actions of damage to nucleic acid, membrane and protein synthesis systems in the seeds, membrane lipid peroxidation, chromosome and gene distortion, degradation of embryonic protein and the like; in addition, during the storage process, the unsaturated fatty acid in the seeds is oxidized to cause the permeability of cell membranes to be increased, and meanwhile, free radicals and peroxides formed by oxidation can damage proteins, membrane structures, cell tissues and the like; malondialdehyde is the final product of the oxidation of unsaturated fatty acid in seeds, can cause serious damage to the membrane system of the seeds, and along with the increase of the content of malondialdehyde, the integrity of the cell membrane structure of the seeds is kept to be reduced, and the shortening degree of the service life is increased. The presence of protection, detoxification, repair systems in the seed is an important mechanism for maintaining longevity. The seed protection system of flavonoid compounds and the like can play a role in protecting by eliminating active oxygen and limiting membrane lipid peroxidation. Seed storage is closely related to detoxification mechanisms and control of pro-oxidative/antioxidant balance. When detoxification mechanisms are affected, seed death inevitably results. Meanwhile, a large number of researches show that due to the spraying of gibberellin and the characteristics of the variety, hybrid rice seeds still keep active physiological metabolism in the late maturing period and the storage period of the seeds, so that partial nutrient substances are consumed, the accumulation of harmful substances is increased, the service life of the seeds is shortened quickly after the harvested seeds are stored for a period of time, the active physiological metabolism can be reduced and slowed down by using proper inhibiting substances for treatment, the negative influence caused by the active physiological metabolism is reduced, and the service life of the seeds is prolonged. Recent studies found that proteins associated with protein synthesis and metabolism of storage-intolerant seeds undergo drastic changes during storage, and studies on yeast longevity and nematode longevity show that inhibition of protein translation can effectively improve longevity.
The speed of the vitality decline of the seeds in the storage process is mainly determined by a genetic mechanism, the environment in the seed production process influences the expression of genetic factors, namely, the change of the life of the rice seeds is also related to factors such as seed moisture, temperature, humidity, ventilation and the like in the storage process, and the influences can be avoided or alleviated by proper adjustment through manual measures. Light, O2, CO2, microorganisms, stored pests, and chemicals such as fungicides used to kill pests and microorganisms can have an effect on seed longevity. The problem of shortened service life of seeds in the storage process is mainly solved through engineering technical approaches at home and abroad for a long time, and the method comprises the steps of constructing a low-temperature drying warehouse and the like, and the service life of a large amount of seeds is reduced and scrapped on the basis of consuming a large amount of manpower, material resources and financial resources, so that the safe storage of the seeds and the quality of the seeds are influenced. The research on the technology of maintaining vitality (i.e. longevity) in hybrid rice seed storage, as well as the technology of packaging, sealing packaging, storage method, fumigation treatment and insect prevention, and the like, are the research hotspots in the field of seeds at present. However, until now, no effective practical technology is applied in production, and even under the condition of refrigeration, the germination rate of the seeds is reduced year by year, and is decreased by more than 3% every year, and the function of the seeds is lost, so that the problems of long-term resource preservation and national reserve of the prepared seeds also exist in the aspect of seed life loss. Therefore, the life of the seeds is prolonged through different ways, which is not only a very worth of discussion in biology, but also has great practical significance in agricultural production and national strategy.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention mainly aims to provide a compound rice seed life prolonging agent. The prolonging agent can be used alone or in combination with field management measures in the late maturing stage of rice to prolong the storage life of rice seeds.
The invention also aims to provide a preparation method of the compound rice seed life prolonging agent.
The invention further aims to provide application of the compound rice seed life prolonging agent.
The purpose of the invention is realized by the following technical scheme:
a composite rice seed life prolonging agent is prepared from the following raw materials in percentage by mass:
preferably, the compound rice seed life prolonging agent is prepared from the following raw materials in percentage by mass:
the vine tea extract is calculated by dihydromyricetin, and the mass percentage content is more than or equal to 50%;
the mass percentage of the bitter almond extract is more than or equal to 50.0 percent calculated by amygdalin;
the mass percentage of the clove oil extract is more than or equal to 50.0 percent calculated by eugenol;
the tea tree essential oil extract is calculated by terpinene and p-menthene-1-ol-4, wherein the mass percentage of the terpinene is 1.5-5%, and the mass percentage of the p-menthene-1-ol-4 is more than 30%;
the mass percentage of the coumarin extract is more than or equal to 10 percent based on the coumarin;
the paclobutrazol accounts for more than 15 percent of the mass percent;
the 72% agricultural streptomycin wettable powder is 72% in mass percentage by weight of streptomycin.
The ampelopsis grossedentata extract is prepared by the following steps: drying fresh stems and leaves of vine tea at the temperature of 35-45 ℃ to obtain vine tea drying raw materials; extracting Ampelopsis grossedentata dry raw materials by adopting an ethanol extraction method to obtain an Ampelopsis grossedentata extracting solution, and then carrying out a spray drying method to obtain an Ampelopsis grossedentata extract; the mass ratio of the ampelopsis grossedentata dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%, and the preferred volume percentage concentration is 90%; the ampelopsis grossedentata is ampelopsis grossedentata produced in spring and summer in Guangdong, Guangxi, Hunan, Hubei, Jiangxi and the like, and is preferably ampelopsis grossedentata produced in Zhang Jiajie region of Hunan in Hunan;
the bitter almond extract is prepared by the following steps: adopting mature bitter almond fruits collected in summer, removing pulp and nucleocapsid, taking out seeds, and drying at 35-45 ℃ to obtain dry bitter almond raw materials; extracting dried semen Armeniacae amarum with ethanol to obtain extractive solution, and spray drying to obtain semen Armeniacae amarum extract; the mass ratio of the dried bitter almond raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 65-95%, and the preferred volume percentage concentration is 70%; the bitter apricot kernel fruit is dried mature seed of Rosaceae plant Prunus armeniaca L.var.ansu Maxim, Western British apricot (Prunus armeniaca L.), northeast apricot Prunus mandshurica (Maxim.) Koehne or apricot Primus armeniaca L.and is preferably apricot northeast;
the clove oil extract is prepared by the following steps: extracting by steam distillation, specifically, putting flos Caryophylli raw material into an extraction tank, adding 6 times volume of water of flos Caryophylli raw material, soaking for 2h, heating to 100 deg.C, distilling, and collecting oil with clean stainless steel barrel when oil and water are separated; putting the primarily distilled oil into a reduced pressure concentration pot, starting a heating valve, starting secondary distillation at the temperature of 70-80 ℃ and under the steam pressure of 0.1MPa, standing the collected volatile oil for 24 hours, and filtering to obtain a clove oil extract;
the tea tree essential oil extract is prepared by the following steps: extracting by adopting a steam distillation method, specifically taking branches and leaves of Melaleuca alternifolia (Melaleuca alternifolia), Melaleuca brassicifolia (Melaleuca brita bracteata), Melaleuca asiatica (Melaleuca erica), Melaleuca quinquenervia (Melaleuca quinquenervia) or Melaleuca viridissima (Melaleuca Viridiflora) in Melaleuca (Myrtaceae) as raw materials, preferably taking branches and leaves of Melaleuca alternifolia as raw materials, putting the raw materials into an extraction tank, adding 6 times volume of water of the raw materials, soaking for 2 hours, heating to 100 ℃, distilling, and collecting oil by using a clean stainless steel barrel when oil and water are separated; putting the primarily distilled oil into a reduced pressure concentration pot, starting a heating valve, starting secondary distillation at 70-80 ℃ under the steam pressure of 0.1MPa, standing the collected volatile oil for 24h, and filtering to obtain the tea tree essential oil extract;
the coumarin extract is prepared by the following steps: the extraction is carried out by utilizing the characteristic that coumarin compounds can be dissolved in hot alkali liquor and separated out by adding acid. The method specifically comprises the following steps: heating and extracting raw materials rich in coumarin compounds with 0.5% sodium hydroxide aqueous solution by mass percentage, cooling the extract, removing impurities with diethyl ether, adjusting pH to neutral with acid, concentrating, acidifying, separating out coumarin and its glycoside, and drying to obtain crystalline coumarin extract; the raw materials rich in coumarin compounds are sweet clover, radix Peucedani, herba Cichorii, radix Angelicae Dahuricae, fructus Psoraleae, black bean or orchid; the mass ratio of the raw materials to the sodium hydroxide aqueous solution is 1: 10-12;
the paclobutrazol is produced by chemical manufacturing company Limited of Jiangxin Hubei, and the mass percentage content is more than 15 percent;
the 72% agricultural streptomycin wettable powder is produced by Sichuan Chengdu general bioengineering limited company, and the mass percentage content is 72%.
The preparation method of the compound rice seed life prolonging agent comprises the following operation steps: weighing all the raw materials according to the proportion, placing the raw materials into a blending tank, uniformly stirring, and packaging or canning the raw materials in a composite aluminum bag to obtain the composite rice seed life extender.
The composite rice seed life prolonging agent is applied to prolonging the life of rice seeds.
The application comprises the following operation steps: in the late stage of the production of the hybrid rice seeds to the stage of the production of the seeds in the milk ripeness stage, the composite rice seed life prolonging agent is stirred and dissolved by water to form direct liquid with the mass percentage of 1-3%, the direct liquid is sprayed on the seeds which are not completely ripened in the field of the production of the hybrid rice seeds according to the dosage of 50-80 kg/mu, the effect of prolonging the life of the rice seeds can be achieved by continuously spraying for 1-2 times, and preferably, the effect of continuously spraying for 2 times is achieved.
The principle of the invention is as follows:
the main active ingredients in the Ampelopsis grossedentata extract are flavanone compounds and flavonoid compounds, wherein dihydromyricetin and myricetin are two main monomer components in the extract. The results of the previous researches show that the vine tea extract has multiple functions of resisting aging, resisting oxidation and the like. The ampelopsis grossedentata extract in the formula has the main functions of preventing nucleic acid, membranes and protein synthesis systems in seeds from being seriously damaged in the storage process of the seeds, reducing the peroxidation of membrane lipid and preventing the generation and accumulation of malondialdehyde.
Bitter almond extract: the main component is amygdalin which is vitamin B17 and is one of the effective components in the traditional Chinese medicine amygdalin, and is widely existed in seeds of rosaceous plants such as peaches, apricots, plums, apples and hawthorns.
Clove oil extract: the main components are phenol compounds such as eugenol and the like, and the formula mainly utilizes the sterilization, corrosion prevention and insect prevention functions of the formula and simultaneously utilizes the function of eliminating lipid free radicals to protect cell membranes of seed embryos so as to prolong the service life of seeds.
Tea tree essential oil extract: the tea tree essential oil has effects of eliminating inflammation, eliminating swelling, treating suppurative wound and burn, sunburn, etc., and can be used as soap, cream, skin caring emulsion, deodorant, disinfectant and air freshener, etc. The tea tree essential oil extract mainly contains terpinene compounds such as terpinene and p-menthene-1-ol-4. The main function of the tea tree essential oil extract in the patent is to reduce the oxidation of unsaturated fatty acid in seeds, thereby reducing the permeability of cell membranes and prolonging the service life of the seeds.
Coumarin extract: the main active component is coumarin compound, and the pharmaceutical effect is to inhibit the synthesis of blood coagulation factors in liver. The coumarin medicine has similar structure with vitamin K. The coumarin drug is combined with vitamin K epoxide reductase in liver to inhibit the conversion of vitamin K from epoxide to hydroquinone, and the circulation of vitamin K is inhibited. The coumarin class of drugs can be said to be vitamin K antagonists, or to be competitive inhibitors. The formula is mainly used for inhibiting the synthesis of alpha-amylase in the late rice maturing process of rice, reducing the content of soluble sugar in the rice, reducing respiration and prolonging the seed life.
Paclobutrazol: is a triazole plant growth regulator successfully developed in 80 years, and is an inhibitor of endogenous gibberellin synthesis. The value of paclobutrazol in agricultural applications lies in its controlling effect on crop growth. Has the effects of delaying plant growth, inhibiting stem elongation, shortening internodes, promoting plant tillering, promoting flower bud differentiation, increasing plant stress resistance, increasing yield, etc. The formula is mainly used for delaying the growth of plants and blocking the effect of Gibberellin (GA) on germination, thereby prolonging the service life of seeds.
Agricultural streptomycin 72% wettable powder: is easily dissolved in water, and is used as antibiotic. The formula is mainly used for killing microbes inside and outside hybrid rice seeds by utilizing the functions of inhibiting protein synthesis and broad-spectrum efficient antibiosis, and reducing various damages caused by the microbes to the seeds, thereby prolonging the service life of the seeds.
Compared with the prior art, the invention has the following advantages and beneficial effects:
(1) the invention has reasonable formula, comprehensively utilizes respective effects of chemical raw materials and extracts in natural plants on prolonging the service life of seeds, and has very obvious effect on prolonging the service life of rice seeds through experimental verification;
(2) simple processing and preparation, convenient use and provides an ideal medicament for prolonging the service life of rice seeds.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
The following examples use the following sources of raw materials:
1. the ampelopsis grossedentata extract is prepared by the following steps: drying fresh stems and leaves of vine tea at the temperature of 35-45 ℃ to obtain vine tea drying raw materials; extracting Ampelopsis grossedentata dry raw materials by adopting an ethanol extraction method to obtain an Ampelopsis grossedentata extracting solution, and then carrying out a spray drying method to obtain an Ampelopsis grossedentata extract; the mass ratio of the ampelopsis grossedentata dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%. The content of ampelopsis grossedentata extract in the obtained ampelopsis grossedentata extract is more than or equal to 50 percent by mass based on dihydromyricetin.
2. The bitter almond extract is prepared by the following steps: adopting a mature bitter almond fruit collected in summer (the bitter almond fruit is dried mature seeds of wild apricot, Siberian apricot, northeast apricot or apricot of Rosaceae), removing pulp and nucleocapsid, taking out seeds, and drying at 35-45 deg.C to obtain dried bitter almond; extracting dried semen Armeniacae amarum with ethanol to obtain extractive solution, and spray drying to obtain semen Armeniacae amarum extract; the ethanol used for ethanol extraction has a volume percentage concentration of 70-80%, and the mass ratio of the raw materials to the ethanol is 1: 10-12, the leaching temperature is 80-85 ℃, and the leaching time is 1 hour. The content of the amygdalin in the extract of the bitter apricot seeds is more than or equal to 50.0 percent by weight.
3. The clove oil extract is prepared by the following steps: extracting by steam distillation, specifically, putting flos Caryophylli raw material into an extraction tank, adding 6 times volume of water of flos Caryophylli raw material, soaking for 2h, heating to 100 deg.C, distilling, and collecting oil with clean stainless steel barrel when oil and water are separated; putting the primarily distilled oil into a reduced pressure concentration pot, starting a heating valve, starting secondary distillation at the temperature of 70-80 ℃ and the steam pressure of 0.1MPa, standing the collected volatile oil for 24 hours, and filtering to obtain the volatile oil. The clove oil extract is calculated by eugenol, and the mass percentage content is more than or equal to 50.0 percent;
4. the tea tree essential oil extract is prepared by the following steps: extracting by adopting a steam distillation method, specifically taking branches and leaves of plants such as Melaleuca alternifolia, Melaleuca fragrans (Melaleuca fragita), Melaleuca americana (Melaleuca erica), white oil tree (Melaleuca quinquervia), Melaleuca viridis (Melaleuca virdiflora) and the like in Melaleuca (Myrtaceae) Melaleuca L, putting the branches and leaves of the Melaleuca alternifolia into an extraction tank, adding 6 times volume of water of the raw material, soaking for 2h, heating to 100 ℃, distilling, and collecting oil by using a clean stainless steel barrel when oil and water are separated; putting the primarily distilled oil into a reduced pressure concentration pot, starting a heating valve, starting secondary distillation at the temperature of 70-80 ℃ and the steam pressure of 0.1MPa, standing the collected volatile oil for 24 hours, and filtering to obtain the volatile oil. The tea tree essential oil extract is calculated by terpinene and p-menthene-1-ol-4, wherein the mass percentage of the terpinene is 1.5-5%, and the mass percentage of the p-menthene-1-ol-4 is more than 30%.
5. The coumarin extract is prepared by the following steps: the method adopts the raw materials rich in coumarin compounds, such as sweet clover, whiteflower hogfennel root, chicory, angelica dahurica, malaytea scurfpea fruit, black tonka bean or orchid, and the like, and utilizes the characteristic that the coumarin compounds can be dissolved in hot alkali liquor and separated out by adding acid for extraction. During actual extraction, heating and extracting by using a sodium hydroxide aqueous solution with the mass percentage concentration of 0.5%, cooling an extracting solution, removing impurities by using ether, then adding acid to adjust the pH value to be neutral, concentrating, acidifying again, separating out coumarin and glycoside thereof, and drying to separate out the crystalline coumarin extract. The mass ratio of the raw materials rich in the coumarin compound to the sodium hydroxide aqueous solution is 1: 10 to 12. The obtained coumarin extract contains at least 10 wt% of total coumarin.
6. Paclobutrazol: produced by chemical manufacturing company Limited of Jiangxin Hubei, the mass percentage content is more than 15 percent in terms of paclobutrazol.
7. Agricultural streptomycin 72% wettable powder: produced by Sichuan Chengdu general bioengineering GmbH, and the mass percentage is 72%.
Example 1:
respectively weighing 8 raw materials of 25.00 mass percent of vine tea extract, 20.00 mass percent of bitter almond extract, 7.50 mass percent of clove oil extract, 12.50 mass percent of tea tree essential oil extract, 20.00 mass percent of coumarin extract, 7.50 mass percent of paclobutrazol and 7.50 mass percent of agricultural streptomycin 72 percent wettable powder, placing the raw materials into a preparation tank, uniformly stirring the raw materials, and packaging or canning the raw materials by a composite aluminum bag to obtain the compound rice seed life extender.
Example 2:
respectively weighing 7 raw materials of 24.50 mass percent of vine tea extract, 20.50 mass percent of bitter almond extract, 7.50 mass percent of clove oil extract, 12.50 mass percent of tea tree essential oil extract, 20.50 mass percent of coumarin extract, 7.00 mass percent of paclobutrazol and 7.50 mass percent of agricultural streptomycin 72 percent wettable powder, placing the raw materials into a blending tank, uniformly stirring the raw materials, and packaging or canning the raw materials by a composite aluminum bag to obtain the compound rice seed life extender.
Example 3: the change situation of the seed life extension of the 368 series hybrid rice seeds after the treatment of the composite rice seed life extender
500g of the compound rice seed life extender prepared in the embodiment 1 is weighed, stirred and dissolved by 50kg of water to form emulsion, and the emulsion is filled in a plastic bucket to obtain the direct use solution of the rice seed life extender with the mass percentage of 1%. The direct liquid is sprayed for 1 time in 4 hybrid rice seed production fields (under the condition of natural growth, the liquid is sprayed at the early stage of rice wax ripening) of Boyou 368, Tianyou 368, Qiyou 368 and IIyou 368, and the spraying dosage is 50 kg/mu. In the experimental process, a control CK without spraying the direct solution is set.
After the seeds are mature, the service life conditions of the seeds produced in the field for producing each series of hybrid rice are respectively determined by an accelerated aging method.
The accelerated seed aging method comprises the following steps: and (3) pretreating the sprayed seeds and the control seeds which are not sprayed for 3 days in a closed dryer with the temperature of 15 ℃ and the relative humidity of 85%, transferring the seeds to a closed dryer with the temperature of 42 ℃ and the relative humidity of 85% for treating for 8 days, and finally drying the seeds in a closed dryer with the temperature of 25 ℃ and the relative humidity of 32% for 3 days, wherein the seed aging treatment is finished. Respectively taking the aged seeds to perform a germination rate experiment; the life prolongation of the seeds is expressed by the numerical value of the germination rate of the seeds after accelerated aging.
As can be seen from Table 1, the effect of prolonging the life of the seeds is significant after 1 time of spraying the rice seed life prolonging agent in the wax ripening stage. The above hybrid rice combination name is provided by Jinmiao Rice industries, Ltd, Guangdong province.
TABLE 1 Change in seed longevity of 368-series hybrid rice plants treated with a seed longevity-extending agent
Example 4: the change condition of seed life extension of 3618 series hybrid rice after being treated by the composite rice seed life extender
Weighing 1.50kg of the compound rice seed life extender prepared in the embodiment 2, stirring and dissolving the compound rice seed life extender into emulsion by using 100kg of water, and filling the emulsion into a plastic bucket to obtain the direct use solution of the seed life extender with the mass percent of 1.5%. The direct solution is sprayed on the experimental fields for producing hybrid rice seeds of Boyou 3618, Tianyou 3618, Qiuyou 3618 and IIyou 3618 for 2 times respectively (the interval is 1 day) in the wax ripening period. The area of each experimental field is 0.1 mu, and the application amount of the liquid is 50 kg/mu per spraying. In the experimental process, a control CK without spraying the direct solution is set. After the seeds are mature, the service life prolonging condition of the seeds produced in the field for producing each series of hybrid rice is respectively determined by an accelerated aging method.
The accelerated seed aging method comprises the following steps: and (3) pretreating the control seeds subjected to spraying treatment and not subjected to spraying treatment in a closed dryer with the temperature of 15 ℃ and the relative humidity of 85% for 3 days, then transferring the control seeds to a closed dryer with the temperature of 42 ℃ and the relative humidity of 85% for treatment for 8 days, finally drying the control seeds in a closed dryer with the temperature of 25 ℃ and the relative humidity of 32% for 3 days, and finishing the aging treatment of the seeds. Respectively taking the aged seeds to perform a germination rate experiment; the longevity prolongation of the seeds is expressed in terms of the germination percentage of the seeds after accelerated aging.
TABLE 2 Change in seed longevity of "3618" series hybrid Rice after treatment with seed longevity-extending agent
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (7)
1. A compound rice seed life prolonging agent is characterized in that: the compound rice seed life prolonging agent is prepared from the following raw materials in percentage by mass:
21.00 to 27.00 percent of vine tea extract
17.00 to 25.00 percent of bitter almond extract
Clove oil extract 5.00-10.00%
10.00 to 15.00 percent of tea tree essential oil extract
17.50-22.50% of coumarin extract
Paclobutrazol 5.00-10.00%
72% wettable powder of agricultural streptomycin 5.00-10.00%
The ampelopsis grossedentata extract is prepared by the following steps: drying fresh stems and leaves of vine tea at the temperature of 35-45 ℃ to obtain vine tea drying raw materials; extracting Ampelopsis grossedentata dry raw materials by adopting an ethanol extraction method to obtain an Ampelopsis grossedentata extracting solution, and then carrying out a spray drying method to obtain an Ampelopsis grossedentata extract; the mass ratio of the ampelopsis grossedentata dry raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 85-95%;
the bitter almond extract is prepared by the following steps: adopting mature bitter almond fruits collected in summer, removing pulp and nucleocapsid, taking out seeds, and drying at 35-45 ℃ to obtain dry bitter almond raw materials; extracting dried semen Armeniacae amarum with ethanol to obtain extractive solution, and spray drying to obtain semen Armeniacae amarum extract; the mass ratio of the dried bitter almond raw material to the ethanol in the ethanol extraction method is 1: 10-12, wherein the volume percentage concentration of the ethanol is 65-95%;
the clove oil extract is prepared by the following steps: extracting by steam distillation, specifically, putting flos Caryophylli raw material into an extraction tank, adding 6 times volume of water of flos Caryophylli raw material, soaking for 2h, heating to 100 deg.C, distilling, and collecting oil with clean stainless steel barrel when oil and water are separated; putting the primarily distilled oil into a reduced pressure concentration pot, starting a heating valve, starting secondary distillation at the temperature of 70-80 ℃ and under the steam pressure of 0.1MPa, standing the collected volatile oil for 24 hours, and filtering to obtain a clove oil extract;
the tea tree essential oil extract is prepared by the following steps: extracting by steam distillation, specifically taking branch and leaf of Melaleuca alternifolia, Melaleuca aleuca, Melaleuca Alternifolia or Melaleuca aleuca of Myrtaceae as raw materials, putting into an extraction tank, adding 6 times volume of water of the raw materials, soaking for 2h, heating to 100 deg.C for distillation, and collecting oil with a clean stainless steel barrel when oil and water are separated; putting the primarily distilled oil into a reduced pressure concentration pot, starting a heating valve, starting secondary distillation at 70-80 ℃ under the steam pressure of 0.1MPa, standing the collected volatile oil for 24h, and filtering to obtain the tea tree essential oil extract;
the coumarin extract is prepared by the following steps: heating and extracting raw materials rich in coumarin compounds with 0.5% sodium hydroxide aqueous solution by mass percentage, cooling the extract, removing impurities with diethyl ether, adjusting pH to neutral with acid, concentrating, acidifying, separating out coumarin and its glycoside, and drying to obtain crystalline coumarin extract; the raw materials rich in coumarin compounds are sweet clover, radix Peucedani, herba Cichorii, radix Angelicae Dahuricae, fructus Psoraleae, black bean or orchid; the mass ratio of the raw materials to the sodium hydroxide aqueous solution is 1: 10 to 12.
2. The compound rice seed longevity extender according to claim 1, wherein: the compound rice seed life prolonging agent is prepared from the following raw materials in percentage by mass:
25.00 percent of vine tea extract
Almond extract 20.00%
Clove oil extract 7.50%
Tea tree essential oil extract 12.50%
Coumarin extract 20.00%
Paclobutrazol 7.50%
Agricultural streptomycin 72% wettable powder 7.50%.
3. The compound rice seed longevity extender according to claim 1, wherein: the vine tea extract is calculated by dihydromyricetin, and the mass percentage content is more than or equal to 50%;
the mass percentage of the bitter almond extract is more than or equal to 50.0 percent calculated by amygdalin;
the mass percentage of the clove oil extract is more than or equal to 50.0 percent calculated by eugenol;
the tea tree essential oil extract is calculated by terpinene and p-menthene-1-ol-4, wherein the mass percentage of the terpinene is 1.5-5%, and the mass percentage of the p-menthene-1-ol-4 is more than 30%;
the mass percentage of the coumarin extract is more than or equal to 10 percent based on the coumarin;
the paclobutrazol accounts for more than 15 percent of the mass percent;
the 72% agricultural streptomycin wettable powder is 72% in mass percentage by weight of streptomycin.
4. The compound rice seed longevity extender according to claim 1, wherein: the paclobutrazol is produced by chemical manufacturing company Limited of Jiangxin Hubei, and the mass percentage content is more than 15 percent;
the 72% agricultural streptomycin wettable powder is produced by Sichuan Chengdu general bioengineering limited company, and the mass percentage content is 72%.
5. The preparation method of the compound rice seed longevity extender according to claim 1, which is characterized by comprising the following steps: weighing all the raw materials according to the proportion, placing the raw materials into a blending tank, uniformly stirring, and packaging or canning the raw materials in a composite aluminum bag to obtain the composite rice seed life extender.
6. The use of the compound rice seed longevity extender according to claim 1 for prolonging the longevity of rice seeds, wherein: the application comprises the following operation steps: in the late stage of the production of the hybrid rice seeds to the stage of the production of the seeds in the milk ripeness stage, the composite rice seed life prolonging agent is stirred and dissolved by water to form direct liquid with the mass percentage of 1-3%, the direct liquid is sprayed on the seeds which are not completely ripened in the field of the production of the hybrid rice seeds according to the dosage of 50-80 kg/mu, and the effect of prolonging the life of the rice seeds is achieved by spraying for 1-2 times.
7. Use according to claim 6, characterized in that: the number of spraying is 2.
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| CN201710711540.4A CN107517955B (en) | 2017-08-18 | 2017-08-18 | Composite rice seed life prolonging agent and preparation method and application thereof |
| PCT/CN2018/098451 WO2019033934A1 (en) | 2017-08-18 | 2018-08-03 | Life-extending agent for hybrid oryza sativa seeds, preparation method therefor and application thereof |
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| CN111569465B (en) * | 2020-05-28 | 2021-11-19 | 广西壮族自治区林业科学研究院 | Coumarin extraction device and use method thereof |
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| CN103039604A (en) * | 2013-01-23 | 2013-04-17 | 广东省农业科学院蚕业与农产品加工研究所 | Complex paddy mould inhibitor made from natural plant extracts, and preparation method of mould inhibitor |
| CN103250695A (en) * | 2013-05-24 | 2013-08-21 | 广东省农业科学院水稻研究所 | Natural plant extract rice seed embryo vigor retention agent as well as preparation method and application thereof |
| CN106106548A (en) * | 2016-06-28 | 2016-11-16 | 广东省农业科学院水稻研究所 | A kind of composite water spike of rice sprouts inhibitor and its preparation method and application |
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| CN106665677A (en) * | 2016-12-04 | 2017-05-17 | 钦州市星火计划办公室 | Rice sprouting inhibitor and preparation method thereof |
| CN107517955B (en) * | 2017-08-18 | 2021-01-26 | 广东省农业科学院农业生物基因研究中心 | Composite rice seed life prolonging agent and preparation method and application thereof |
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| CN103039604A (en) * | 2013-01-23 | 2013-04-17 | 广东省农业科学院蚕业与农产品加工研究所 | Complex paddy mould inhibitor made from natural plant extracts, and preparation method of mould inhibitor |
| CN103250695A (en) * | 2013-05-24 | 2013-08-21 | 广东省农业科学院水稻研究所 | Natural plant extract rice seed embryo vigor retention agent as well as preparation method and application thereof |
| CN106106548A (en) * | 2016-06-28 | 2016-11-16 | 广东省农业科学院水稻研究所 | A kind of composite water spike of rice sprouts inhibitor and its preparation method and application |
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