CN107502643A - A kind of colored glue polypeptide production methods - Google Patents
A kind of colored glue polypeptide production methods Download PDFInfo
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- CN107502643A CN107502643A CN201710907667.3A CN201710907667A CN107502643A CN 107502643 A CN107502643 A CN 107502643A CN 201710907667 A CN201710907667 A CN 201710907667A CN 107502643 A CN107502643 A CN 107502643A
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- Prior art keywords
- glue
- flower
- rubber cement
- colored
- sauce
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000003292 glue Substances 0.000 title claims abstract description 79
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 23
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 21
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 21
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 12
- 239000004365 Protease Substances 0.000 claims abstract description 65
- 108091005804 Peptidases Proteins 0.000 claims abstract description 38
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 38
- 235000019419 proteases Nutrition 0.000 claims abstract description 38
- 239000004568 cement Substances 0.000 claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 37
- 150000001875 compounds Chemical class 0.000 claims abstract description 30
- 235000015067 sauces Nutrition 0.000 claims abstract description 30
- 108090000526 Papain Proteins 0.000 claims abstract description 27
- 235000019834 papain Nutrition 0.000 claims abstract description 27
- 229940055729 papain Drugs 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 239000006228 supernatant Substances 0.000 claims abstract description 16
- 229940088598 enzyme Drugs 0.000 claims abstract description 15
- 230000009849 deactivation Effects 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 230000007062 hydrolysis Effects 0.000 claims abstract description 9
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 9
- 239000000047 product Substances 0.000 claims abstract description 8
- 238000009835 boiling Methods 0.000 claims abstract description 7
- 239000000284 extract Substances 0.000 claims abstract description 7
- 238000010792 warming Methods 0.000 claims abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 3
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 8
- 238000004140 cleaning Methods 0.000 claims description 6
- 239000003513 alkali Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 230000003301 hydrolyzing effect Effects 0.000 claims description 4
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 3
- 238000001694 spray drying Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 241000219112 Cucumis Species 0.000 description 2
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 2
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 244000189799 Asimina triloba Species 0.000 description 1
- 235000006264 Asimina triloba Nutrition 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 210000004712 air sac Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004833 fish glue Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to a kind of colored glue polypeptide production methods, comprise the following steps that：Step 1）, by flower glue cleaned；Step 2）, flower glue blend into uniform flower glue sauce；Step 3）, flower glue sauce add the water of 24 times of flower glue sauce weight, obtain spending rubber cement liquid；Step 4）, regulation flower rubber cement liquid pH value be 25, boiling 24 hours at a temperature of 60 90 °C, be then cooled to 50 °C, add papain, compound protease stirring hydrolysis；Step 5）, papain, compound protease be warming up to flower rubber cement liquid mixed liquor not less than 100 °C, and enzyme deactivation in 20 30 minutes is kept at a temperature of not less than 100 °C, obtains the hydrolyzate after enzyme deactivation；Step 6）, hydrolyzate is centrifuged, remove upper-layer fat, extract supernatant；Step 7）, supernatant is dried to powder, produce colored glue polypeptide products.By the present invention, the difficult fully extraction of albumen in the flower glue processed, there is very big market application value.
Description
Technical field
The present invention relates to a kind of colored glue polypeptide production methods, belong to colored glue processing technique field.
Background technology
Flower glue is the dried product of air bladder, also referred to as fish maw, fish glue, is reason rich in advanced collagen and multivitamin
The high protein and low fat food thought；But existing colored glue eating method is simple, the albumen in flower glue is difficult to separation and Extraction.In recent years
Carry out people and collagen peptide and bone calcium etc. are extracted in various fish-skins, fish scale, but the report prepared to flower glue polypeptide compares
It is rare.
The content of the invention
The purpose of the present invention be for it is above-mentioned now exist the problem of, there is provided it is a kind of be easy to spend glue nutrient absorption flower glue it is more
Peptide preparation method.
The object of the present invention is achieved like this, a kind of colored glue polypeptide production methods, it is characterized in that, including following making step
Suddenly：
Step 1）, prepare flower glue, by flower glue cleaned；
Step 2）, the flower glue after cleaning blended into uniform flower glue sauce；
Step 3）, will be through step 2）The flower glue sauce of acquisition adds the water of 2-4 times of flower glue sauce weight, and flower glue sauce and water stirring is equal
It is even, obtain spending rubber cement liquid；
Step 4）, regulation flower rubber cement liquid pH value be 2-5, the boiling 2-4 hours at a temperature of 60-90 °C, be then cooled to 50 °C,
Papain, compound protease are added, papain accounts for 0.1-0.3 times of colored rubber cement liquid, and compound protease accounts for colored rubber cement
0.2-0.3 times of liquid, papain, compound protease and flower rubber cement liquid stirring hydrolysis 20-30 hours；
Step 5）, the papain after hydrolyzing 20-30 hours, compound protease and colored rubber cement liquid mixed liquor will be stirred be warming up to
Not less than 100 °C, and 20-30 minute enzyme deactivations are kept at a temperature of not less than 100 °C, obtain the hydrolyzate after enzyme deactivation；
Step 6）, will be through step 5）Obtained hydrolyzate is centrifuged, and removes upper-layer fat, extracts supernatant；
Step 7）, by supernatant vacuum freeze drying or spraying drying powder-forming, produce colored glue polypeptide products.
The step 3）In, flower glue sauce and water stirring 0.5-1 hours.
The step 4）In, compound protease is in papain, neutral proteinase, alkali protease, trypsase
It is any two or more.
The step 6）In, supernatant is dried to powder and uses vacuum freeze drying or spray drying.
The advanced science of present invention process, by the present invention, flower glue polypeptide predominantly prepares flower glue when making, by flower glue
Cleaned；Then the flower glue after cleaning is blended into uniform flower glue sauce；Then the flower glue sauce obtained, which is added to the water, to be stirred
To flower rubber cement liquid；And the pH value for adjusting colored rubber cement liquid is 2-5, the boiling 2-4 hours at a temperature of 60-90 °C, is then cooled to 50 °
C, add papain, compound protease, papain, compound protease and flower rubber cement liquid stirring hydrolysis 20-30 hours；
Then the papain after hydrolyzing 20-30 hours will be stirred, compound protease is warming up to flower rubber cement liquid mixed liquor and is not less than
100 °C, and 20-30 minute enzyme deactivations are kept at a temperature of not less than 100 °C, obtain the hydrolyzate after enzyme deactivation；Then will obtain
Hydrolyzate be centrifuged, remove upper-layer fat, extract supernatant；By supernatant vacuum freeze drying or spray drying
Cheng Fen, produce colored glue polypeptide products.
To sum up, by the present invention, the difficult fully extraction of albumen in the flower glue processed, there is very big market to apply valency
Value.
Embodiment
Embodiment 1
A kind of colored glue polypeptide production methods, are comprised the following steps that：
Step 1）, prepare flower glue, by flower glue cleaned；
Step 2）, the flower glue after cleaning blended into uniform flower glue sauce；
Step 3）, will be through step 2）The flower glue sauce of acquisition adds the water of 2 times of flower glue sauce weight, and flower glue sauce is stirred with water,
Flower glue sauce stirs 0.5 hour with water, obtains spending rubber cement liquid；
Step 4）, regulation flower rubber cement liquid pH value be 2, boiling 2 hours at a temperature of 60 °C, be then cooled to 50 °C, add wood
Melon protease, compound protease, papain account for 0.1 times of colored rubber cement liquid, and compound protease accounts for 0.2 times of colored rubber cement liquid,
Papain, compound protease and flower rubber cement liquid stirring hydrolysis 20 hours, wherein, compound protease be papain, in
It is any two or more in property protease, alkali protease, trypsase；
Step 5）, the papain after hydrolysis 20 hours will be stirred, compound protease is warming up to colored rubber cement liquid mixed liquor
101 °C, and enzyme deactivation in 20 minutes is kept at a temperature of not less than 101 °C, obtain the hydrolyzate after enzyme deactivation；
Step 6）, will be through step 4）Obtained hydrolyzate is centrifuged, and removes upper-layer fat, extracts supernatant；
Step 7）, by supernatant vacuum freeze drying or spraying drying powder-forming, produce colored glue polypeptide products.
Embodiment 2
A kind of colored glue polypeptide production methods, are comprised the following steps that：
Step 1）, prepare flower glue, by flower glue cleaned；
Step 2）, the flower glue after cleaning blended into uniform flower glue sauce；
Step 3）, will be through step 2）The flower glue sauce of acquisition adds the water of 3 times of flower glue sauce weight, and flower glue sauce is stirred with water,
Flower glue sauce stirs 0.7 hour with water, obtains spending rubber cement liquid；
Step 4）, regulation flower rubber cement liquid pH value be 2-5, the boiling 2-4 hours at a temperature of 75 °C, be then cooled to 50 °C, add
Enter papain, compound protease, papain accounts for 0.2 times of colored rubber cement liquid, and compound protease accounts for colored rubber cement liquid
0.25 times, papain, compound protease and flower rubber cement liquid stirring hydrolysis 25 hours, wherein, compound protease is pawpaw egg
It is any two or more in white enzyme, neutral proteinase, alkali protease, trypsase；
Step 5）, the papain after hydrolysis 25 hours will be stirred, compound protease is warming up to colored rubber cement liquid mixed liquor
103 °C, and enzyme deactivation in 25 minutes is kept at a temperature of 103 °C, obtain the hydrolyzate after enzyme deactivation；
Step 6）, will be through step 4）Obtained hydrolyzate is centrifuged, and removes upper-layer fat, extracts supernatant；
Step 7）, by supernatant vacuum freeze drying or spraying drying powder-forming, produce colored glue polypeptide products.
Embodiment 3
A kind of colored glue polypeptide production methods, are comprised the following steps that：
Step 1）, prepare flower glue, by flower glue cleaned；
Step 2）, the flower glue after cleaning blended into uniform flower glue sauce；
Step 3）, will be through step 2）The flower glue sauce of acquisition adds the water of 4 times of flower glue sauce weight, and flower glue sauce is stirred with water,
Flower glue sauce stirs 1 hour with water, obtains spending rubber cement liquid；
Step 4）, regulation flower rubber cement liquid pH value be 5, boiling 4 hours at a temperature of 90 °C, be then cooled to 50 °C, add wood
Melon protease, compound protease, papain account for 0.3 times of colored rubber cement liquid, and compound protease accounts for 0.3 times of colored rubber cement liquid,
Papain, compound protease and flower rubber cement liquid stirring hydrolysis 30 hours, wherein, compound protease be papain, in
It is any two or more in property protease, alkali protease, trypsase；
Step 5）, the papain after hydrolyzing 20-30 hours, compound protease and colored rubber cement liquid mixed liquor will be stirred be warming up to
105 °C, and 20-30 minute enzyme deactivations are kept at a temperature of not less than 105 °C, obtain the hydrolyzate after enzyme deactivation；
Step 6）, will be through step 4）Obtained hydrolyzate is centrifuged, and removes upper-layer fat, extracts supernatant；
Step 7）, by supernatant vacuum freeze drying or spraying drying powder-forming, produce colored glue polypeptide products.
Claims (4)
1. a kind of colored glue polypeptide production methods, it is characterized in that, comprise the following steps that：
Step 1）, prepare flower glue, by flower glue cleaned；
Step 2）, the flower glue after cleaning blended into uniform flower glue sauce；
Step 3）, will be through step 2）The flower glue sauce of acquisition adds the water of 2-4 times of flower glue sauce weight, and flower glue sauce and water stirring is equal
It is even, obtain spending rubber cement liquid；
Step 4）, regulation flower rubber cement liquid pH value be 2-5, the boiling 2-4 hours at a temperature of 60-90 °C, be then cooled to 50 °C,
Papain, compound protease are added, papain accounts for 0.1-0.3 times of colored rubber cement liquid, and compound protease accounts for colored rubber cement
0.2-0.3 times of liquid, papain, compound protease and flower rubber cement liquid stirring hydrolysis 20-30 hours；
Step 5）, the papain after hydrolyzing 20-30 hours, compound protease and colored rubber cement liquid mixed liquor will be stirred be warming up to
Not less than 100 °C, and 20-30 minute enzyme deactivations are kept at a temperature of not less than 100 °C, obtain the hydrolyzate after enzyme deactivation；
Step 6）, will be through step 5）Obtained hydrolyzate is centrifuged, and removes upper-layer fat, extracts supernatant；
Step 7）, by supernatant vacuum freeze drying or spraying drying powder-forming, produce colored glue polypeptide products.
2. a kind of colored glue polypeptide production methods according to claim 1, it is characterized in that, the step 3）In, flower glue sauce with
Water stirs 0.5-1 hours.
3. a kind of colored glue polypeptide production methods according to claim 1, it is characterized in that, the step 4）In, compound protein
Enzyme is any two or more in papain, neutral proteinase, alkali protease, trypsase.
4. a kind of colored glue polypeptide production methods according to claim 1, it is characterized in that, the step 6）In, by supernatant
It is dried to powder and uses vacuum freeze drying or spray drying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710907667.3A CN107502643A (en) | 2017-09-29 | 2017-09-29 | A kind of colored glue polypeptide production methods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710907667.3A CN107502643A (en) | 2017-09-29 | 2017-09-29 | A kind of colored glue polypeptide production methods |
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| Publication Number | Publication Date |
|---|---|
| CN107502643A true CN107502643A (en) | 2017-12-22 |
Family
ID=60699902
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710907667.3A Withdrawn CN107502643A (en) | 2017-09-29 | 2017-09-29 | A kind of colored glue polypeptide production methods |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108936691A (en) * | 2018-05-31 | 2018-12-07 | 张志霞 | Flower glue extracting solution and preparation method thereof, collagen peptide food of beautifying face and moistering lotion comprising the flower glue extracting solution and preparation method thereof |
| CN111528467A (en) * | 2020-05-28 | 2020-08-14 | 大洲新燕(厦门)生物科技有限公司 | Fish gelatin and bird's nest porridge and preparation method thereof |
| CN112715867A (en) * | 2020-12-28 | 2021-04-30 | 山东蓝奥生物技术有限公司 | Preparation method of flower glue and flower glue |
| CN113088550A (en) * | 2021-05-14 | 2021-07-09 | 葛兰瑞克(厦门)食品科技有限公司 | Fish collagen peptide and preparation method thereof |
| CN115281319A (en) * | 2022-07-12 | 2022-11-04 | 山东蓝奥生物技术有限公司 | Flower collagen liquid and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101545001A (en) * | 2008-03-30 | 2009-09-30 | 段振华 | Method for preparing swimbladder oligopeptide solution |
| CN103789381A (en) * | 2014-02-14 | 2014-05-14 | 钟春燕 | Method for rapidly extracting fish collagen |
| CN104313098A (en) * | 2014-10-28 | 2015-01-28 | 威海市桢昊生物技术有限公司 | Method for preparing swimming bladder collagen powder |
-
2017
- 2017-09-29 CN CN201710907667.3A patent/CN107502643A/en not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101545001A (en) * | 2008-03-30 | 2009-09-30 | 段振华 | Method for preparing swimbladder oligopeptide solution |
| CN103789381A (en) * | 2014-02-14 | 2014-05-14 | 钟春燕 | Method for rapidly extracting fish collagen |
| CN104313098A (en) * | 2014-10-28 | 2015-01-28 | 威海市桢昊生物技术有限公司 | Method for preparing swimming bladder collagen powder |
Non-Patent Citations (3)
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|---|
| 刘永峰等: "鱼胶原蛋白提取方法研究进展", 《食品工业》 * |
| 孙燕丽等: "鱼鳔胶原蛋白活性肤的研究进展", 《生物技术与计算机科学研究》 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108936691A (en) * | 2018-05-31 | 2018-12-07 | 张志霞 | Flower glue extracting solution and preparation method thereof, collagen peptide food of beautifying face and moistering lotion comprising the flower glue extracting solution and preparation method thereof |
| CN111528467A (en) * | 2020-05-28 | 2020-08-14 | 大洲新燕(厦门)生物科技有限公司 | Fish gelatin and bird's nest porridge and preparation method thereof |
| CN112715867A (en) * | 2020-12-28 | 2021-04-30 | 山东蓝奥生物技术有限公司 | Preparation method of flower glue and flower glue |
| CN113088550A (en) * | 2021-05-14 | 2021-07-09 | 葛兰瑞克(厦门)食品科技有限公司 | Fish collagen peptide and preparation method thereof |
| CN115281319A (en) * | 2022-07-12 | 2022-11-04 | 山东蓝奥生物技术有限公司 | Flower collagen liquid and preparation method thereof |
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Application publication date: 20171222 |