CN107494989A - The man-made feeds formula and processing method of a kind of hickie cuttlefish - Google Patents

Abstract

The invention discloses a kind of man-made feeds formula of hickie cuttlefish and processing method, processing method to comprise the following steps：Extraction fish guts oil, fish oil is modified, prepares liposome, compounding, the separation and Extraction Application of Viscera Oil from fresh fishes internal organ, enzymatic modification is carried out through desaturase and reduced Coenzyme II, the man-made feeds of hickie cuttlefish are then re-dubbed with liposome, amino acid, vitamin etc..Have the beneficial effect that：Raw material used are the accessory substance or discarded object of industrial and agricultural production, not only reduce cost, and the full effect for realizing material utilizes；Feed nutrition material general equilibrium prepared by the inventive method, is easy to the absorption of hickie cuttlefish, strengthens body defenses function, improves the diseases prevention of body, resistance against diseases, resisting stress, anti-mutagenesis ability, suppresses pathogenic bacterial infection, reduce the incidence of disease of hickie cuttlefish；Feed quality is stable, is not easy to go mouldy, is not easy to be oxidized, Storage period limit for length, be easy to transport, can apply to industrial production, has vast potential for future development.

Description

The man-made feeds formula and processing method of a kind of hickie cuttlefish

Technical field

The present invention relates to aquaculture feeds manufacture field, a kind of man-made feeds formula more particularly, to hickie cuttlefish and add Work method.

Technical background

Hickie Sepia Cephalopoda, Sepiidae.Metastomium peltate, trunk length are about wide 2 times of trunk, and trunk back of the body central area has many big Small alternate greyish white color spot, both sides have some short horizontal stripes.Record maximum trunk long 0.5m, weight limit 10kg.Hickie cuttlefish For the species that build in Sepiidae is maximum, northwest Pacific littoral sea is distributed in, key operation fishing ground is in the Ryukyu Islands with Guangdong coastal waters of state, can eat raw with it is drying.It is one of famous marine product in China, in Zhejiang, and large yellow croaker, little yellow croaker, Hairtail is referred to as " four big economic fish ", deep to be liked by consumers in general.Not only sense of taste is fresh and crisp tasty and refreshing for hickie cuttlefish, and protein contains Amount is high, has higher nutritive value, and rich in medical value.Hickie cuttlefish is dry and the dry stewing soup of mung bean eats and plays improving eyesight drop The health-care effects such as fire, " cuttlefish plate " scientific name are the medicinal material commonly used in the traditional Chinese medical science again, claimed " cuttlebone " " cuttle bone ".

Hickie cuttlefish delicious flavour, it is nutritious, 13 grams of protein, only 0.7 gram of fat, also containing carbon water are contained per hectogram meat The material necessary to human body such as compound and vitamin A, vitamin B complex and calcium, phosphorus, iron, riboflavin.Ancient Chinese medicine doctor thinks, in vain Spot cuttlefish nature and flavor are sweet, salty, flat, there is all effects of nourishing liver kidney, nourishing the blood and yin, QI invigorating.Hickie cuttlefish is precious from head to foot, is first-class dietotherapy A kind of rather preferable health food of good merchantable brand, especially women, though woman's all one's life through, pregnant, production, breast each phase, eat inkfish all To be beneficial, according to records, women is edible have blood-nourishing, improving eyesight, stimulate the menstrual flow, be antiabortive, sharp production, hemostasis, lactagogue and uterine bleeding and other effects.

Cuttlefish was once one of China's traditional " four big marine products ", and because its meat flavour is delicious, nutritive value is high, is always regarded as by people Marine treasure, in recent years because the mankind largely catch, cause cuttlefish resource increasingly exhausted, therefore develop man-made feeds technology and just become Obtain more important.

At present, the feed processing technology on cuttlefish has many methods, and prior art is as authorized public number

The B of CN 102232488 Chinese invention patent, discloses Sepiella maindroni basal bait, and this method is by by aquatic products Meat gruel, casein, sodium chloride and 10 kinds of amino acid are mixed and made into the foundation bait of Sepiella maindroni, have technique simply excellent Point, but the bait only contains amino acid and protein, can not provide the required vitamin of cuttlefish growth, mineral matter, growth-promoting The materials such as long agent, antioxidant, preservative, adhesive are also short of in bait, causes feed easily putrid and deteriorated, cuttlefish growth hair Educate slowly, nutrient imbalance, or even phenomena such as disease, dead occur, therefore be unworthy promoting.

The content of the invention

It is an object of the invention to provide a kind of man-made feeds of the cheap hickie cuttlefish environmentally friendly, nutrition is balanced of cost to match somebody with somebody Side and processing method.

The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：A kind of artificial feeding of hickie cuttlefish Material formula and processing method, it is formed and its parts by weight are：Organic fertilizer 90-100 parts, soybean cake 50-60 parts, liposome 5-10 Part, fish guts oil 40-50 parts, fish protein hydrolyzate 20-30 parts, split kettle algae 10-15 parts, chlorella 10-15 parts, shrimp shell meal 2.5- 3 parts, brewer's yeast 2-2.5 parts, dextrin 12-15 parts, alphalise starch 14-18 parts, calcium dihydrogen phosphate 2.0-3.0 parts, urea 20-25 Part, ammonium nitrate 15-16 parts, calcium phosphate 10-12 parts, Choline Chloride 0.5-0.7 parts, threonine 0.45-0.48 parts, glutamic acid 1.2- 1.4 parts, proline 2.00-2.15 parts, valine 0.35-0.40 parts, methionine 0.30-0.34 parts, leucine 0.60-0.68 Part, tyrosine 0.35-0.37 parts, lysine 0.42-0.45 parts, arginine 1.19-1.23 parts, tryptophan 0.4-0.5 parts, dimension life Plain A0.3-0.4 parts, vitamin B 0.5-0.7 parts, vitamin D 0.1-0.2 parts, vitamin E 0.3-0.4 parts, yeast cell wall 3-4 Part, sodium alginate 1-2 parts, enzyme preparation 1.5-1.7 parts, antioxidant 0.5-0.7 parts, growth accelerator 5-6 parts, preservative 0.08- 0.1 part, specifically include following steps：

Extract fish guts oil：Fresh fishes internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner Slurry, the homogenate of 200-210 parts fish guts is weighed into beaker, adds 60-65 part distilled water, stir lower warming-in-water to 30-34 DEG C, 10% potassium hydroxide solution regulation pH to 8-9 is added, continues stirring and is warming up to 45-55 DEG C, be incubated 25-35 minutes, add in fish Saltout after dirty heavy 5-7% potassium chloride stirring and dissolving and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil；Siphon crude fish oil is used in combination Hot water wash 3-5 times, centrifuge while hot, take supernatant liquid, fish guts oil is drying to obtain with vacuum drying chamber；Fish guts oil rich in DHA, The HUFA such as EPA, the nutriment of cuttlefish feed is highly suitable as, and this method fish oil yield is high, it is simple to operate, waste water, Waste residue can be made into green high-efficient fertilizer total integration utilization, be also used as extract nucleic acid raw material it is further high-valued, reach The purpose of waste's reclamation；

Fish oil is modified：Fish guts oil 85-95 parts, distilled water 100-120 parts, vitamin E 0.1-0.11 parts are weighed in closed container In be well mixed, reaction vessel is placed in Ultrasound Instrument, 65-68 DEG C is heated to and keeps, is continually fed into oxygen 5-8 minutes, so Add desaturase 0.3-0.4 parts, reduced Coenzyme II 0.05-0.06 parts afterwards, power be 400-500W, frequency 80- It is stand-by after the 90KHz praetersonic effect lower reaction 110-140 seconds；Siphon enzymatic fish oil and with hot water wash 3-5 times, while hot from The heart, supernatant liquid is taken, fish guts oil is drying to obtain with vacuum drying chamber；Enzymatic reaction can be by the long-chain interposition of saturated fatty acid The hydrogen for putting carbon-carbon single bond seizes generation water by oxygen, so that chain saturated fatty acids lose hydrogen and are converted into corresponding unsaturated fat Acid, so that squid body can be changed after being fed to hickie cuttlefish rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil The composition of interior lipid and aliphatic acid, blood fat in squid body can be adjusted, strengthen eyesight, improved immunity, be highly suitable as white Spot cuttlefish feed, when desaturase and the mass ratio of reduced Coenzyme II are 6-7:When 1, enzymatic reaction activity highest, reaction is most To be thorough；

Prepare liposome：Weigh cholesterol 30-32 parts, soybean lecithin 100-110 parts, Tea Polyphenols 2-2.5 parts, vitamin C 15-20 Part, the ethanol solution of 500-550 parts 50% is added at a temperature of 15-18 DEG C, being passed through under conditions of nitrogen makes all substances completely molten Solution, rotary evaporation, which removes absolute ethyl alcohol, can obtain one layer of uniform lipid membrane, and adding 200-220 part ether makes Film Fractionation, then The phosphate buffer that 60-80 parts pH is 6.4-6.6 is added, is sheared under 16000-18000r/min rotating speeds with high-speed shearing machine 5-7 minutes, that is, O/W emulsions are made, then rotating pressure-decreasing evaporation removing ether, stirring 10-15 minutes are under the conditions of 20-25 DEG C Liposome；Liposome encapsulation prepared by the method is high, when Tea Polyphenols and ascorbic weight ratio are 1:7-8, Tea Polyphenols with Vitamin has complexing, is not easy to be oxidized destruction before as feed additive entering in squid body, it is stronger to preserve VC Anti-oxidation characteristics, into squid body in after cuttlefish can be promoted to grow and regulation of physiological functions；The phosphatide that this method makes Liposome is easy to the absorption of cuttlefish, and preparation method is simple, is avoided in preparation process containing potentially harmful substances such as ether Physics and chemistry processing, be especially suitable for industrialization large-scale production；

Compounding：Weigh organic fertilizer 90-100 parts, soybean cake 50-60 parts, liposome 5-10 parts, fish guts oil 40-50 parts, fish egg White hydrolysate 20-30 parts, split kettle algae 10-15 parts, chlorella 10-15 parts, shrimp shell meal 2.5-3 parts, brewer's yeast 2-2.5 parts, paste Smart 12-15 parts, alphalise starch 14-18 parts, calcium dihydrogen phosphate 2.0-3.0 parts, urea 20-25 parts, ammonium nitrate 15-16 parts, calcium phosphate 10-12 parts, Choline Chloride 0.5-0.7 parts, threonine 0.45-0.48 parts, glutamic acid 1.2-1.4 parts, proline 2.00-2.15 Part, valine 0.35-0.40 parts, methionine 0.30-0.34 parts, leucine 0.60-0.68 parts, tyrosine 0.35-0.37 parts, Lysine 0.42-0.45 parts, arginine 1.19-1.23 parts, tryptophan 0.4-0.5 parts, vitamin A 0.3-0.4 parts, vitamin B0.5-0.7 parts, vitamin D 0.1-0.2 parts, vitamin E 0.3-0.4 parts, yeast cell wall 3-4 parts, sodium alginate 1-2 parts, enzyme Preparation 1.5-1.7 parts, antioxidant 0.5-0.7 parts, growth accelerator 5-6 parts, preservative 0.08-0.1 parts, powder after combination drying It is broken, suitable quantity of water input granulating disc granulation, particle diameter 2-3mm are added after crossing 30-40 mesh sieves, drying particle produces hickie cuttlefish Artificial feeding feed；Strengthen hickie cuttlefish immune system defense function, improve diseases prevention, the resistance against diseases of body, improve aquatic products The resisting stress of animal, anti-mutagenesis, suppress pathogenic bacterial infection, reduce the incidence of disease of hickie cuttlefish；Stimulate internal system, regulation new Old metabolism, efficiency of feed utilization is improved to promote the growth of hickie cuttlefish, improve hickie cuttlefish and profit is digested and assimilated to feed nutrient With rate, feed coefficient is reduced, reduces nitrogen, the excretion of phosphorus, reduces water pollution；Feed stability is improved, is extended shelf life, can So that applied to industrial production, there is vast potential for future development.

Preferably, the fresh fishes are perch or bighead or silver carp；The internal organ unrighted acid of three kinds of fish Content is higher, and it is dropped more in process, therefore fish guts oil obtains rate height, and realizes full effect and utilize.

Preferably, the organic fertilizer is chicken manure or pig manure or cow dung；Organic fertilizer can provide abundant for cuttlefish Organic matter and middle trace element, promote body growth development.

Preferably, the enzyme preparation is pectase or 1,4 beta-glucanase or phytase or high activity zytase or fiber Plain enzyme or neutral proteinase；Digest and absorb rate of the cuttlefish to feed nutrient is improved, reduces feed coefficient；Reduce nitrogen, phosphorus Excretion, water pollution is reduced, reduce the incidence of disease of aquatic livestock.

Preferably, the antioxidant is ethoxyquin beautiful jade or dibutyl hydroxy toluene or butylated hydroxy anisole or Du Zhong Ye powder or Honegsukle flower P.E；Antioxidant can effectively improve feed stability and extend shelf life.

Preferably, the growth accelerator is allicin or the Radix Astragali or radix bupleuri or XIGANCAO or haw thorn extract or dandelion Extract；Growth accelerator can be by stimulating internal system, regulation metabolism, improving efficiency of feed utilization to promote cuttlefish Growth.

Preferably, the mould inhibitor is potassium propionate or sodium benzoate or sodium Diacetate；Mould inhibitor can prevent feed mold Caused feed nutrient missing, palatability decline, or even cause mycotoxicosis.

Compared with prior art, the advantage of the invention is that：1）Raw material used are the accessory substance of industrial and agricultural production Or discarded object, cost is not only reduced, and the full effect for realizing material utilizes, and protects environment；2）By by Tea Polyphenols with Vitamin C is prepared into phospholipid liposome by extra fine quality ratio, and can have effectively prevents vitamin C to be oxidized degraded, into cuttlefish Its anti-oxidation efficacy can be effectively played after in vivo, and phosphatide can also provide nutriment for organism；3）The present invention Feed nutrition material general equilibrium prepared by method, it is easy to the absorption of hickie cuttlefish, strengthens body defenses function, improve body Diseases prevention, resistance against diseases, resisting stress, anti-mutagenesis, the suppression pathogenic bacterial infection of aquatic livestock are improved, reduces the morbidity of hickie cuttlefish Rate；4）Feed quality is stable, is not easy to go mouldy, is not easy to be oxidized, Storage period limit for length, is easy to transport, can apply to industrial production, With vast potential for future development.

Brief description of the drawings

Fig. 1 is 1-3 of the embodiment of the present invention and control group hickie cuttlefish feed experimental data schematic diagram；

Fig. 2 is 1-3 of the embodiment of the present invention and the content of fatty acid schematic diagram in control group；

Fig. 3 is thio lauric anhydride and enzymatic preparation under the conditions of the different quality ratio of L- (+) -2,3- dihydroxypropionic acids in the present invention The content schematic diagram of middle cis unsaturated fatty acid.

Embodiment

The present invention program is described further below by embodiment：

Embodiment 1：

The man-made feeds formula and processing method of a kind of hickie cuttlefish, it is formed and its parts by weight are：90 parts of cow dung, soybean cake 50 Part, 5 parts of liposome, fish guts oil 40 parts, 20 parts of fish protein hydrolyzate, split 10 parts of kettle algae, 10 parts of chlorella, 2.5 parts of shrimp shell meal, 2 parts of brewer's yeast, 12 parts of dextrin, 14 parts of alphalise starch, 2.0 parts of calcium dihydrogen phosphate, 20 parts of urea, 15 parts of ammonium nitrate, calcium phosphate 10 Part, 0.5 part of Choline Chloride, 0.45 part of threonine, 1.2 parts of glutamic acid, 2.00 parts of proline, 0.35 part of valine, methionine 0.30 part, 0.60 part of leucine, 0.35 part of tyrosine, 0.42 part of lysine, 1.19 parts of arginine, 0.4 part of tryptophan, vitamin A0.3 parts, 0.5 part of vitamin B, 0.1 part of vitamin D, 0.3 part of vitamin E, 3 parts of yeast cell wall, 1 part of sodium alginate, pectin 1.5 parts of enzyme, 0.5 part of butylated hydroxy anisole, 5 parts of dandelion extract, 0.08 part of potassium propionate, specifically include following steps：

1）200 parts of perch internal organ homogenate are weighed into beaker, add 60 parts of distilled water, stir lower warming-in-water to 30 DEG C, regulation PH to 8, continue stirring and be warming up to 45 DEG C, be incubated 25 minutes, after being saltoutd 20 minutes after 10 parts of potassium chloride stirring and dissolvings of addition while hot Centrifugation, separates to obtain crude fish oil；Siphon crude fish oil and with hot water wash 3 times, centrifuges, takes supernatant liquid, done with vacuum drying chamber while hot It is dry to produce fish guts oil；Fish guts oil is rich in the HUFA such as DHA, EPA, is highly suitable as the nutriment of cuttlefish feed；2）Claim 30 parts of cholesterol, 100 parts of soybean lecithin, 2 parts of Tea Polyphenols, 15 parts of vitamin C are taken, 500 part 50% of second is added at a temperature of 15 DEG C Alcoholic solution, being passed through under conditions of nitrogen is completely dissolved all substances, rotary evaporation remove absolute ethyl alcohol can obtain one layer it is uniform Lipid membrane, adding 200 parts of ether makes Film Fractionation, adds 60 parts of sodium phosphate buffers, is used under 16000r/min rotating speeds High-speed shearing machine is sheared 5 minutes, that is, O/W emulsions are made, and then rotating pressure-decreasing evaporation removes ether, stirring 10 under the conditions of 20 DEG C The liposome of minute i.e.；Liposome encapsulation prepared by the method is high, before entering as feed additive in squid body not Easily be oxidized and destroy, preserve the stronger anti-oxidation characteristics of VC, into squid body in after cuttlefish can be promoted to grow and adjust Physiological function；3）Each composition and its parts by weight are weighed, are crushed after combination drying, addition suitable quantity of water input disk is made after crossing 30 mesh sieves Grain machine is granulated, particle diameter 2mm, and drying particle produces the artificial feeding feed of hickie cuttlefish；Enhancing hickie cuttlefish immune system is prevented Imperial function, diseases prevention, the resistance against diseases of body are improved, improve resisting stress, anti-mutagenesis, the suppression pathogenic bacterial infection of aquatic livestock, drop The incidence of disease of low hickie cuttlefish.

Embodiment 2：

The man-made feeds formula and processing method of a kind of hickie cuttlefish, specifically include following steps：

1）Extract fish guts oil：210 parts of bighead internal organ homogenate are weighed into beaker, add 65 parts of distilled water, stir lower water-bath liter Temperature adjusts pH to 9 to 34 DEG C, continues stirring and is warming up to 55 DEG C, be incubated 35 minutes, adds salt after 14.7 parts of potassium chloride stirring and dissolvings Analysis centrifuges while hot after 30 minutes, separates to obtain crude fish oil；Siphon crude fish oil and with hot water wash 5 times, centrifuges, takes supernatant liquid while hot, Fish guts oil is drying to obtain with vacuum drying chamber；This method fish oil yield is high, simple to operate, and waste water, waste residue can be made into green height Imitate fertilizer total integration to utilize, it is further high-valued to be also used as extracting the raw material of nucleic acid, has reached waste's reclamation Purpose；

2）Fish oil is modified：95 parts of fish guts oil, 120 parts of distilled water, 0.11 part of vitamin E is weighed to mix in closed container It is even, reaction vessel is placed in Ultrasound Instrument, 68 DEG C is heated to and keeps, be continually fed into oxygen 8 minutes, then add desaturase 0.4 part, 0.06 part of reduced Coenzyme II, it is stand-by after react 140 seconds under praetersonic effect after siphon enzymatic fish oil and with hot Washing 5 times, while hot centrifugal drying produce fish guts oil；Enzymatic reaction can be by the long-chain centre position carbon-carbon single bond of saturated fatty acid Hydrogen generation water is seized by oxygen so that chain saturated fatty acids lose hydrogen and are converted into corresponding unrighted acid so that Rich in polyunsaturated fatty acids such as DHA, EPA in fish guts oil；

3）Prepare liposome：32 parts of cholesterol, 110 parts of soybean lecithin, 2.5 parts of Tea Polyphenols, 0 part of Catergen are weighed, at 18 DEG C At a temperature of add 550 part 50% of ethanol solution, being passed through under conditions of nitrogen is completely dissolved all substances, and rotary evaporation removes Absolute ethyl alcohol can obtain one layer of uniform lipid membrane, and adding 220 parts of ether makes Film Fractionation, add the phosphorus that 80 parts of pH are 6.6 Phthalate buffer, sheared 7 minutes with high-speed shearing machine under 18000r/min rotating speeds, that is, O/W emulsions are made, then in 25 DEG C of bars Rotation, which is evaporated under reduced pressure, under part removes ether, 15 minutes liposomes i.e. of stirring；The phospholipid liposome that this method makes is easy to cuttlefish Absorption, and preparation method is simple, the physics and chemistry processing containing potentially harmful substances such as ether is avoided in preparation process, very It is adapted to industrialization large-scale production；

4）Compounding：Weigh 100 parts of chicken manure, 60 parts of soybean cake, 10 parts of liposome, fish guts oil 50 parts, 30 parts of fish protein hydrolyzate, Split 15 parts of kettle algae, 15 parts of chlorella, 3 parts of shrimp shell meal, 2.5 parts of brewer's yeast, 15 parts of dextrin, 18 parts of alphalise starch, calcium dihydrogen phosphate 3.0 parts, 25 parts of urea, 16 parts of ammonium nitrate, 12 parts of calcium phosphate, 0.7 part of Choline Chloride, 8 parts of amino acid, 1.7 parts of vitamin, yeast 4 parts of cell membrane, 2 parts of sodium alginate, 1.7 parts of cellulase, 0.7 part of Eucommia Leaf Powder, 6 parts of allicin, 0.1 part of sodium Diacetate, mix Crushed after being dried is closed, adds suitable quantity of water input granulating disc granulation after crossing 40 mesh sieves, particle diameter 3mm, drying particle produces white The artificial feeding feed of spot cuttlefish；Strengthen hickie cuttlefish immune system defense function, improve diseases prevention, the resistance against diseases of body, carry The resisting stress of high aquatic livestock, anti-mutagenesis, suppress pathogenic bacterial infection, reduce the incidence of disease of hickie cuttlefish；Feed stability is improved, Extend shelf life, can apply to industrial production, there is vast potential for future development.

Embodiment 3：

The man-made feeds formula and processing method of a kind of hickie cuttlefish, it is formed and its parts by weight are：100 parts of organic fertilizer, beans 55 parts of grouts, 8 parts of liposome, 45 parts of fish guts oil, 20 parts of fish protein hydrolyzate, split 12 parts of kettle algae, 12 parts of chlorella, shrimp shell meal 2.5 parts, 2.5 parts of brewer's yeast, 15 parts of dextrin, 18 parts of alphalise starch, 3.0 parts of calcium dihydrogen phosphate, 25 parts of urea, 16 parts of ammonium nitrate, 12 parts of calcium phosphate, 0.7 part of Choline Chloride, 0.48 part of threonine, 1.4 parts of glutamic acid, 2.15 parts of proline, 0.40 part of valine, 0.34 part of methionine, 0.68 part of leucine, 0.37 part of tyrosine, 0.45 part of lysine, 1.23 parts of arginine, tryptophan 0.5 Part, 0.4 part of vitamin A, 0.7 part of vitamin B, 0.2 part of vitamin D, 0.4 part of vitamin E, 4 parts of yeast cell wall, sodium alginate 2 parts, 1 part of pectase, 0.7 part of 1,4 beta-glucanase, 0.2 part of dibutyl hydroxy toluene, 0.5 part of Honegsukle flower P.E, 3 parts of radix bupleuri, 3 parts of XIGANCAO, 0.1 part of sodium Diacetate, specifically include following steps：

Extract fish guts oil：Fresh perch internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, it is even in refiner Slurry, 200 parts of fish guts homogenate are weighed into beaker, add 60 parts of distilled water, stir lower warming-in-water to 32 DEG C, add 10% Potassium hydroxide solution adjusts pH to 8, continues stirring and is warming up to 50 DEG C, be incubated 30 minutes, after adding 12 parts of potassium chloride stirring and dissolvings Centrifuged while hot after saltouing 25 minutes, separate to obtain crude fish oil；Siphon crude fish oil and with hot water wash 4 times, centrifuges, takes upper liquid while hot Body, fish guts oil is drying to obtain with vacuum drying chamber；Fish guts oil is highly suitable as cuttlefish feeding rich in HUFA such as DHA, EPA The nutriment of material, and this method fish oil yield is high, and simple to operate, it is completely comprehensive that waste water, waste residue can be made into green high-efficient fertilizer Close utilize, be also used as extract nucleic acid raw material it is further high-valued, reached the purpose of waste's reclamation；

Fish oil is modified：Propylene-glycol ethyl ether 11.0g, acrylic resin are modified casein 2.30g, polyurethane-modified cellulose nitrate successively 2.50g, weigh vitamin E 0.16g, ethylenediamine tetra-acetic acid 0.50g, L- (+) -2,3- dihydroxypropionic acids 0.015g, thio bay Acid anhydrides 0.105g is subsequently placed in 7500r/min high-speed mixer in mixed at high speed plastic cup and mixes 3.5 minutes to obtain coating Preparation, take coating agent to be evenly applied to closed reaction vessel inner bottom surface and inwall, dried at a temperature of 30 DEG C 20 minutes it is stand-by； Weigh 90 parts of fish guts oil, 110 parts of distilled water, 0.1 part of vitamin E to be well mixed in closed container, reaction vessel is placed in In Ultrasound Instrument, it is heated to 65 DEG C and keeps, be continually fed into oxygen 6 minutes, it is auxiliary then adds 0.350 part of desaturase, reduced form II 0.055 parts of enzyme, it is stand-by after being reacted 120 seconds under the praetersonic effect that power is 450W, frequency is 80KHz；Siphon enzymatic Fish oil and with hot water wash 4 times, centrifuges, takes supernatant liquid, fish guts oil is drying to obtain with vacuum drying chamber while hot；Enzymatic reaction energy The hydrogen of the long-chain centre position carbon-carbon single bond of saturated fatty acid is seized into generation water by oxygen, so that chain saturated fatty acids lose Hydrogen is converted into corresponding unrighted acid, so that being fed in fish guts oil rich in polyunsaturated fatty acids such as DHA, EPA The composition of lipid and aliphatic acid in squid body can be changed after hickie cuttlefish, blood fat in squid body can be adjusted, strengthen eyesight, carry High immunity, it is highly suitable as hickie cuttlefish feed；

Prepare liposome：32 parts of cholesterol, 100 parts of soybean lecithin, 2 parts of Tea Polyphenols, 16 parts of vitamin C are weighed, in 16 DEG C of temperature Lower to add 500 part 50% of ethanol solution, being passed through under conditions of nitrogen is completely dissolved all substances, and rotary evaporation removes anhydrous Ethanol can obtain one layer of uniform lipid membrane, and adding 200 parts of ether makes Film Fractionation, add the phosphate that 60 parts of pH are 6.5 Buffer solution, sheared 5 minutes with high-speed shearing machine under 16000r/min rotating speeds, that is, O/W emulsions are made, then under the conditions of 20 DEG C Rotating pressure-decreasing evaporation removes ether, 10 minutes liposomes i.e. of stirring；Liposome encapsulation prepared by the method is high, works as Tea Polyphenols It is 1 with ascorbic weight ratio:8, Tea Polyphenols has complexing with vitamin, enters as feed additive in squid body Be not easy to be oxidized destruction before, preserve the stronger anti-oxidation characteristics of VC, into squid body in after cuttlefish can be promoted to grow And regulation of physiological functions；The phospholipid liposome that this method makes is easy to the absorption of cuttlefish, and preparation method is simple, preparation process In avoid containing potentially harmful substances such as ether physics and chemistry processing, be especially suitable for industrialization large-scale production；

Compounding：Weigh 100 parts of organic fertilizer, 55 parts of soybean cake, 8 parts of liposome, fish guts oily 45 parts, fish protein hydrolyzate 20 Part, split 12 parts of kettle algae, 12 parts of chlorella, 2.5 parts of shrimp shell meal, 2.5 parts of brewer's yeast, 15 parts of dextrin, 18 parts of alphalise starch, di(2-ethylhexyl)phosphate 3.0 parts of hydrogen calcium, 25 parts of urea, 16 parts of ammonium nitrate, 12 parts of calcium phosphate, 0.7 part of Choline Chloride, 0.48 part of threonine, glutamic acid 1.4 Part, 2.15 parts of proline, 0.40 part of valine, 0.34 part of methionine, 0.68 part of leucine, 0.37 part of tyrosine, lysine 0.45 part, 1.23 parts of arginine, 0.5 part of tryptophan, 0.4 part of vitamin A, 0.7 part of vitamin B, 0.2 part of vitamin D, vitamin E0.4 parts, 4 parts of yeast cell wall, 2 parts of sodium alginate, 1 part of pectase, 0.7 part of 1,4 beta-glucanase, dibutyl hydroxy toluene 0.2 Part, 0.5 part of Honegsukle flower P.E, 3 parts of radix bupleuri, 3 parts of XIGANCAO, 0.1 part of sodium Diacetate, are crushed after combination drying, cross 30 mesh sieves Add suitable quantity of water input granulating disc afterwards to be granulated, particle diameter 3mm, drying particle produces the artificial feeding feed of hickie cuttlefish； Strengthen hickie cuttlefish immune system defense function, improve diseases prevention, the resistance against diseases of body, improve the resisting stress of aquatic livestock, resist Mutagenesis, suppress pathogenic bacterial infection, reduce the incidence of disease of hickie cuttlefish；Stimulate internal system, regulation metabolism, improve feed Utilization rate promotes the growth of hickie cuttlefish, improves digest and absorb rate of the hickie cuttlefish to feed nutrient, reduces bait system Number, nitrogen, the excretion of phosphorus are reduced, reduce water pollution；Feed stability is improved, extends shelf life, can apply to industrial life Production, has vast potential for future development.

Control group：

50 mesh sieves are crushed by buying from the small-sized fish meal of Tianjin Chen Hui feed corporation,Ltds, add suitable quantity of water to put into disc granulation Machine is granulated, particle diameter 3mm, as a control group feed.

Artificial feeding is carried out to hickie cuttlefish with the feed of embodiment 1-3 and control group, feeds 3-4 times day, accomplishes as far as possible A small amount of more throwings, with cuttlefish from food is robbed to food is not robbed as degree, with the increase of body weight, the later stage gradually reduces feeding rate, four groups of holding The regulation of identical feeding and feeding environment are tested, survival rate and the growth rate of cuttlefish carry out statistical analysis, and experimental data is as schemed 1, as seen from Figure 1, feed nutrition material general equilibrium prepared by the inventive method, it is easy to the absorption of hickie cuttlefish, implements Cuttlefish survival rate and rate of body weight gain in example 1-3 are all significantly higher than control group, illustrate the Feed Energy enhancing cuttlefish machine of the inventive method Body defense function, diseases prevention, the resistance against diseases of body are improved, improve its resisting stress, anti-mutagenesis, suppress pathogenic bacterial infection, reduce white The incidence of disease of spot cuttlefish, the death rate is reduced, promote hickie cuttlefish to grow, therefore be a kind of hickie cuttlefish efficiently, safe Man-made feeds formula.

Content of fatty acid measure is carried out to the feed in the fish oil and control group in embodiment 1-3 respectively, wherein, pass through GB/T5532-2008 iodine number method determines the content of unrighted acid, by trans unrighted acid in infrared spectrum Certain bits be equipped with absworption peak, quantitative analysis can be done, so as to draw the content of cis unsaturated fatty acid, experimental data is as schemed Shown in 2, figure it is seen that after purification and desaturation process, content of fatty acid, insatiable hunger in embodiment 1-3 fish oil With content of fatty acid be noticeably greater than control group, and in embodiment 3 for enzymatic reaction carried out coating process be modified after, Its enzymatic reaction and the generation of cis-product have obtained significantly being lifted, and cause the content of EPA and DHA in its product to exceed Embodiment 1,2, feed to the nutritional need that the culture of hickie cuttlefish after hickie cuttlefish, can be met, can change cuttlefish lipid and Aliphatic acid forms, improve its speed of growth, survival rate, immunity with stress resistivity, therefore to the artificial feeding of hickie cuttlefish Support important in inhibiting.

Embodiment 4：

Fish oil is modified with the following steps：

Reaction vessel is coating modified：Weigh propylene-glycol ethyl ether 11.0g successively respectively, acrylic resin is modified casein 2.30g, third The ester modified butadiene resin 2.50g of olefin(e) acid, vitamin E 0.16g, sodium ethylene diamine tetracetate 0.50g, L- (+) -2,3- dihydroxy third Sour 0.02g, thio lauric anhydride in 30 mixed at high speed plastic cups, the quality of thio lauric anhydride be followed successively by 0.100g, 0.102g, 0.104g ..., 0.158g, 0.160g, i.e., the mass ratio of thio lauric anhydride and L- (+) -2,3- dihydroxypropionic acids For 5.0:1、5.1:1、5.2:1、……7.9:1、8.0:1；Then mixing cup will be told to be respectively placed in 9000r/min high speed and mix Coating agent is mixed 3 minutes to obtain in conjunction machine, takes coating agent to be evenly applied to closed reaction vessel inner bottom surface and inwall, at 30 DEG C At a temperature of dry 20 minutes it is stand-by；

Extract fish guts oil：The fresh grass carp harslet homogenate of 200g is taken to add 60g distilled water into beaker, stir lower warming-in-water To 30 DEG C, pH to 8.5 is adjusted, continues stirring and is warming up to 50 DEG C, be incubated 30 minutes, add salt after 12g potassium chloride stirring and dissolving Centrifuged while hot after analysis 20-30 minutes, separate to obtain crude fish oil；Siphon crude fish oil and with hot water wash 3-5 times, centrifuges, takes upper strata while hot Liquid, fish guts oil is drying to obtain with vacuum drying chamber；

Fish oil is modified：Fish guts oil 90g, distilled water 100g, vitamin C 0.11g is weighed to be well mixed in closed container, will be anti- Answer container to be placed in Ultrasound Instrument, be heated to 65 DEG C and keep, be continually fed into oxygen 5 minutes, then add desaturase 0.3g, go back Prototype codehydrogenase Ⅱ 0.05g, it is stand-by after being reacted 120 seconds under the praetersonic effect that power is 460W, frequency is 88KHz；Siphon Enzymatic fish oil and with hot water wash 4 times, centrifuges, takes supernatant liquid, fish guts oil is drying to obtain with vacuum drying chamber while hot；

The different quality ratio of thio lauric anhydride and L- (+) -2,3- dihydroxypropionic acids is measured with Fourier transform infrared spectrogram method Under the conditions of in enzymatic preparation cis unsaturated fatty acid content, experimental result is as shown in figure 3, as seen from Figure 3, enzymatic production The content of cis unsaturated fatty acid thio lauric anhydride and L- (+) -2,3- dihydroxypropionic acids in by reaction vessel coating in thing Mass ratio has a great influence, when thio lauric anhydride with L- (+) -2,3- dihydroxypropionic acid mass ratioes between 6.0：1-6.9:1 area When interior, the content of cis unsaturated fatty acid is higher in product, and content has exceeded 30%, especially, when mass ratio between 6.7:1-6.8：When 1, the content of cis unsaturated fatty acid has exceeded 50% in product, this artificial feeding for hickie cuttlefish The fortification of feed has deep meaning；From the figure 3, it may be seen that in enzymatic preparation the content of cis unsaturated fatty acid with it is thio Non-linear dependencies are presented with L- (+) -2,3- dihydroxypropionic acids mass ratio in lauric anhydride, and it reacts oligodynamics mechanism even not It is bright.

Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.

Technical scheme is described in detail embodiment described above, it should be understood that it is described above only For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention, Supplement or similar fashion replacement etc., should be included in the scope of the protection.

Claims (10)

1. a kind of man-made feeds formula of hickie cuttlefish, it is formed and its parts by weight are：Organic fertilizer 90-100 parts, soybean cake 50-60 parts, liposome 5-10 parts, fish guts oil 40-50 parts, fish protein hydrolyzate 20-30 parts, split kettle algae 10-15 parts, chlorella 10-15 parts, shrimp shell meal 2.5-3 parts, brewer's yeast 2-2.5 parts, dextrin 12-15 parts, alphalise starch 14-18 parts, calcium dihydrogen phosphate 2.0-3.0 parts, urea 20-25 parts, ammonium nitrate 15-16 parts, calcium phosphate 10-12 parts, Choline Chloride 0.5-0.7 parts, threonine 0.45-0.48 parts, glutamic acid 1.2-1.4 parts, proline 2.00-2.15 parts, valine 0.35-0.40 parts, methionine 0.30- 0.34 part, leucine 0.60-0.68 parts, tyrosine 0.35-0.37 parts, lysine 0.42-0.45 parts, arginine 1.19-1.23 Part, tryptophan 0.4-0.5 parts, vitamin A 0.3-0.4 parts, vitamin B 0.5-0.7 parts, vitamin D 0.1-0.2 parts, vitamin E0.3-0.4 parts, yeast cell wall 3-4 parts, sodium alginate 1-2 parts, enzyme preparation 1.5-1.7 parts, antioxidant 0.5-0.7 parts, rush Growth stimulator 5-6 parts, preservative 0.08-0.1 parts.

A kind of 2. man-made feeds formula of hickie cuttlefish according to claim 1, it is characterised in that：Described organic fertilizer For chicken manure or pig manure or cow dung；Described enzyme preparation be pectase or 1,4 beta-glucanase or phytase or high activity zytase or Cellulase or neutral proteinase；Described antioxidant is ethoxyquin beautiful jade or dibutyl hydroxy toluene or butylhydroxy fennel Ether or Eucommia Leaf Powder or Honegsukle flower P.E；Described growth accelerator is that allicin or the Radix Astragali or radix bupleuri or XIGANCAO or hawthorn carry Take thing or dandelion extract；Described mould inhibitor is potassium propionate or sodium benzoate or sodium Diacetate.

A kind of 3. processing method of the man-made feeds formula of hickie cuttlefish, it is characterised in that：Described processing method includes following Step：

1）Extract fish guts oil：The separation and Extraction Application of Viscera Oil from fresh fishes internal organ；

2）Fish oil is modified：Fish oil is modified with enzymatic reaction；

3）Prepare liposome：Liposome is prepared as raw material using the Tea Polyphenols of special ratios and vitamin C；

4）Compounding：Compound the man-made feeds of hickie cuttlefish.

A kind of 4. processing method of the man-made feeds formula of hickie cuttlefish according to claim 3, it is characterised in that：It is described Extraction fish guts oil step be：Fresh fishes internal organ are taken, the digestion residue in removing the internal organs, cleans and drains, in refiner Middle homogenate, the homogenate of 200-210 parts fish guts is weighed into beaker, adds 60-65 part distilled water, stir lower warming-in-water to 30- 34 DEG C, 10% potassium hydroxide solution regulation pH to 8-9 is added, continues stirring and is warming up to 45-55 DEG C, be incubated 25-35 minutes, add Enter to saltout after fish guts weight 5-7% potassium chloride stirring and dissolving and centrifuge while hot after 20-30 minutes, separate to obtain crude fish oil；The thick fish of siphon Oil and with hot water wash 3-5 times, centrifuges, takes supernatant liquid, it is oily to be drying to obtain fish guts with vacuum drying chamber while hot.

A kind of 5. processing method of the man-made feeds formula of hickie cuttlefish according to claim 3, it is characterised in that：It is described Extraction fish guts oil step in fish be perch or bighead or silver carp.

A kind of 6. processing method of the man-made feeds formula of hickie cuttlefish according to claim 3, it is characterised in that：It is described Fish oil modification procedure be：Fish guts oil 85-95 parts, distilled water 100-120 parts, vitamin E 0.1-0.11 parts are weighed in closed It is well mixed in container, reaction vessel is placed in Ultrasound Instrument, 65-68 DEG C is heated to and keeps, is continually fed into 5-8 points of oxygen Clock, the 0.3-0.4 parts of desaturase II, reduced Coenzyme II 0.05-0.06 parts are then added, be 400-500W, frequency in power To be stand-by after the 80-90KHz praetersonic effect lower reaction 110-140 seconds；Siphon enzymatic fish oil and with hot water wash 3-5 times, takes advantage of Heat centrifugation, takes supernatant liquid, and fish guts oil is drying to obtain with vacuum drying chamber.

A kind of 7. processing method of the man-made feeds formula of hickie cuttlefish according to claim 3, it is characterised in that：It is described Fish oil modification procedure in desaturase and the mass ratio of reduced Coenzyme II be 6-7:1.

A kind of 8. processing method of the man-made feeds formula of hickie cuttlefish according to claim 3, it is characterised in that：It is described The liposome step for preparing be：Weigh cholesterol 30-32 parts, soybean lecithin 100-110 parts, Tea Polyphenols 2-2.5 parts, vitamin C15-20 parts, the ethanol solution of 500-550 parts 50% is added at a temperature of 15-18 DEG C, being passed through under conditions of nitrogen makes all substances It is completely dissolved, rotary evaporation, which removes absolute ethyl alcohol, can obtain one layer of uniform lipid membrane, and adding 200-220 part ether makes film molten Solution, the phosphate buffer that 60-80 parts pH is 6.4-6.6 is added, high speed shear is used under 16000-18000r/min rotating speeds Machine shears 5-7 minutes, that is, O/W emulsions are made, and then rotating pressure-decreasing evaporation removes ether under the conditions of 20-25 DEG C, stirs 10-15 The liposome of minute i.e..

A kind of 9. processing method of the man-made feeds formula of hickie cuttlefish according to claim 3, it is characterised in that：It is described Prepare Tea Polyphenols and ascorbic mass ratio in liposome step be 1:7-8.

A kind of 10. processing method of the man-made feeds formula of hickie cuttlefish according to claim 3, it is characterised in that：Institute The compounding step stated is：Weigh organic fertilizer 90-100 parts, soybean cake 50-60 parts, liposome 5-10 parts, fish guts oil 40-50 Part, fish protein hydrolyzate 20-30 parts, split kettle algae 10-15 parts, chlorella 10-15 parts, shrimp shell meal 2.5-3 parts, brewer's yeast 2- 2.5 parts, dextrin 12-15 parts, alphalise starch 14-18 parts, calcium dihydrogen phosphate 2.0-3.0 parts, urea 20-25 parts, ammonium nitrate 15-16 Part, calcium phosphate 10-12 parts, Choline Chloride 0.5-0.7 parts, threonine 0.45-0.48 parts, glutamic acid 1.2-1.4 parts, proline 2.00-2.15 parts, valine 0.35-0.40 parts, methionine 0.30-0.34 parts, leucine 0.60-0.68 parts, tyrosine 0.35-0.37 parts, lysine 0.42-0.45 parts, arginine 1.19-1.23 parts, tryptophan 0.4-0.5 parts, vitamin A 0.3- 0.4 part, vitamin B 0.5-0.7 parts, vitamin D 0.1-0.2 parts, vitamin E 0.3-0.4 parts, yeast cell wall 3-4 parts, marine alga Sour sodium 1-2 parts, enzyme preparation 1.5-1.7 parts, antioxidant 0.5-0.7 parts, growth accelerator 5-6 parts, preservative 0.08-0.1 parts, mix Crushed after being dried is closed, adds suitable quantity of water input granulating disc granulation, particle diameter 2-3mm after crossing 30-40 mesh sieves, drying particle is Obtain the man-made feeds of hickie cuttlefish.