CN107493957A - A kind of implantation methods of mushroom - Google Patents
A kind of implantation methods of mushroom Download PDFInfo
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- CN107493957A CN107493957A CN201710591947.8A CN201710591947A CN107493957A CN 107493957 A CN107493957 A CN 107493957A CN 201710591947 A CN201710591947 A CN 201710591947A CN 107493957 A CN107493957 A CN 107493957A
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- bag
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- mycelia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Mycology (AREA)
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- Mushroom Cultivation (AREA)
Abstract
The invention discloses a kind of implantation methods of mushroom, comprise the following steps：Step 1, compost configuration；The culture medium prescription of mushroom one-level kind is 250 parts of the potato of peeling, 25 parts of glucose, 20 parts of agar, clear water；Major ingredient is the second-generation culture medivm formula of wood chip, is wood chip 78% according to mass fraction ratio, wheat bran 20%, land plaster 1%, sucrose 1%, material-water ratio 1:1.2；Step 2, pack；Step 3, sterilizing；Step 4, inoculation；Step 5, bacterium germination；Step 6, take off bag processing；Step 7, mycelia annesl；Step 8, management of producing mushroom：Creation that must be artificial after annesl is cold and hot, dry and wet and light and shade condition stimulate mycelia, make mycelia constantly break up with it is expanded, mushroom former base is formed and the temperature of fruiting body differentiation is at 10 20 degrees Celsius, keeps the corresponding humidity of air 90% or so.
Description
Technical field
The present invention relates to fungus growing technique field, more particularly to a kind of implantation methods of mushroom.
Background technology
Mushroom is the important edible fungus variety that current depth a kind of in the world is liked by consumer, and one of China's special product,
In the title among the people for being known as " mountain delicacy ".It is a kind of fungi being grown on timber.Mushroom be with high protein, low fat, polysaccharide,
Several amino acids and multivitamin mushroom food, it is often edible to strengthen resistance of human body, contribute to children's bone and tooth
Dental development, while prevent the elderly and suffer from osteoporosis.Mushroom is sweet mild-natured, has the effect of QI invigorating qi-restoratives, strengthening the spleen and stomach, can be with
The cholesterol level in blood is reduced, blood circulation is remained normal, improves heart and brain microcirculation blood supply.Its delicious flavour, fragrance
Ooze people.In addition, mushroom is rich in vitamin B complex, iron, potassium, provitamin D (vitamin D is changed into after Exposure to Sunlight), amino acid up to 18
Kind.But current mushroom implantation methods excessively fall behind, and have impact on the yield and quality of mushroom.
The content of the invention
To solve above mentioned problem present in prior art and actual conditions, the invention provides the implantation methods of mushroom,
It is characterised in that it includes following steps：Step 1, compost configuration；The culture medium prescription of mushroom one-level kind is the Ma Ling of peeling
250 parts of potato, 25 parts of glucose, 20 parts of agar, clear water；Major ingredient is the second-generation culture medivm formula of wood chip, according to mass fraction ratio
For, wood chip 78%, wheat bran 20%, land plaster 1%, sucrose 1%, material-water ratio 1:1.2；
Step 2, pack：Compost prepared by step 1 loads polyethylene plastic bag, the drift angle side of being folded into of cultivating bag
Shape, then fed with charger, during charging, the hand entrusted is firmly uniform, make the compost compactness in bag consistent, if
It is hand charging, it is necessary to while charging, is compacted on one side, material is loaded at 3/5 in bag, cultivating bag requires, bag long 33-55cm, wide
17-30cm, per packed 1-1.5 kilograms of siccative；
Step 3, sterilizing：When the temperature of bacterium bag is naturally cooled to below 25 degrees Celsius, can starts to be inoculated with；
Step 4, inoculation：Cultivating bag enters in inoculating hood along track, carries out ultraviolet light, under ultraviolet irradiation,
First inoculation bottle is opened, removes the mycoderm on strain surface with inoculation cream, and digs up the old mycelia on strain upper strata, then extracts sack
On tampon, strain is accessed in cultivating bag rapidly, has changed tampon, in seeded process, strain bottleneck will be at any time close to alcolhol burner
Burning things which may cause a fire disaster, also to be crossed on alcolhol burner burning things which may cause a fire disaster when tampon is pulled up, ensure that whole seeded process is not contaminated；
Step 5, bacterium germination：Bacterium germination band after inoculation will move into the bacterium germination room that temperature is 25 degrees Celsius in time, restocking training
Support, cultivating bag wants close-packed arrays on frame, to produce the temperature that can keep mycelial growth, cultural hypha 85d, covers with full bag, sends out
It is bred as ripe；
Step 6, take off bag processing：Sack is opened, pushs plastic sheeting aside, take off bag when, temperature no more than 25 degrees Celsius,
It is maintained at less than 22 degrees Celsius；
Step 7, mycelia annesl：Mycelia slowly becomes pink by white, then becomes sepia, and strong light stimulus can be accelerated
Annesl, at 22-25 degrees Celsius, air humidity will strengthen daylight light irradiation after 85% or so, de- bag for temperature control.
Step 8, management of producing mushroom：Creation that must be artificial after annesl is cold and hot, dry and wet and light and shade condition stimulate mycelia, makes
Mycelia constantly differentiation and it is expanded, mushroom former base formed and fruiting body differentiation temperature at 10-20 degrees Celsius, keep air respective wet
Degree is 90%.
Beneficial effect：
A kind of implantation methods of mushroom provided in an embodiment of the present invention, it is easy to learn, and the production of mushroom can be significantly improved
Amount and quality, be high yield and volume production mushroom effective ways, highly popularization and application.
Embodiment
Technical solution of the present invention is described in detail below.
Embodiment 1
Embodiment of the invention discloses that a kind of implantation methods of mushroom, the culture medium prescription of mushroom one-level kind is Ma Ling
Potato (peeling) 250g, glucose 25g, agar 20g, clear water 1000ml.Either, analysis for soybean powder 40g, glucose 20g, agar 20g,
Clear water 1000ml.
Major ingredient is the second-generation culture medivm formula of wood chip：Wood chip 78%, wheat bran 20%, land plaster 1%, sucrose 1%, material water
Than 1:1.2.
The Suitable ranges of mushroom mycelium growth are 5-32 degrees Celsius, and optimum temperature is 22-25 degrees Celsius, and air is relative
Humidity is advisable with 70%.
The selection of cultivation season
Mushroom belongs to low form mushroom, but mushroom mycelium growth course non-refractory, is usually chosen in and connects in 8-10 months
Kind cultivation.
Further, the selection of mushroom culture medium and formula are as follows：
The conventional major ingredient of mushroom culture has, wood chip, cotton seed hulls, bagasse etc..Before dispensing, now wood chip is fermented, killed
Dead various miscellaneous bacterias, are then cleaned with sieve, debris are shone, and are used, and the plant formulation of wood chip is：Wood chip 78%, wheat
Bran or rice bran 20%, land plaster 1%, sucrose 1%, material-water ratio 1:1.2.
Carry out spice on the ground in clean water mud ground or plank, during with mixer spice, by wood chip, wheat bran, land plaster,
Sucrose is placed on respective hopper, is transferred in proportion in wood chip, is then added appropriate water and is stirred together.Mushroom mycelium is given birth to
When long, water content is advisable with 50%-55%.PH value is advisable with 5.5-6, if culture medium is in alkalescence, can add 3% hydrochloric acid solution
Neutralized.Ensuring that PH is more higher during spice is advisable, because its PH can decline after general spice.To prevent living contaminants,
When summer prepares culture medium, 0.1% carbendazim can be added, good effect can be played.
Pack sterilizing
Before pack, sealing experiment is done to cultivating bag with water, using the sack of good seal, damaged sack can cause miscellaneous
Bacterium pollutes.The drift angle of cultivating bag is now folded into squarely, then fed with charger, during charging, the hand entrusted is firmly equal
It is even, make the compost compactness in bag consistent, if hand charging is, it is necessary to which while charging, while being compacted, material is loaded in bag
At 3/5, cultivating bag requirement, bag long 33-55cm, wide 17-30cm, per packed 1-1.5 kilograms of siccative.In sack set after charging
Upper paper ring (hard brown paper), it is with plastics that ring bag is tight, prick into sack, stayed inoculation mouth to be stoppered to be inoculated with inoculation mouth
Tampon, it should be completed from spice pack to sterilizing within 6h, during sterilizing, sack will be discharged rationally, and each sack reserves gap
0.6-1.2cm, it is heated evenly culture medium.Autoclaving, pressure are that 0.014Mpa keeps 2h；Normal-pressure sterilization, at 100 degrees Celsius
Lower holding 10-12h.
Inoculation
Cultivating bag by sterilization treatment is sent into transfer room, is cooled to 25 degrees centigrades, and carry out disinfection inoculation, and sterilization is used
Formaldehyde and potassium permanganate are carried out, and can be inoculated with after stifling 45 minutes.During inoculation, cultivating bag enters inoculating hood along track
It is interior, ultraviolet light is carried out, under ultraviolet irradiation, first inoculation bottle is opened, removes the mycoderm on strain surface with inoculation cream, and
Dig up the old mycelia on strain upper strata, then extract the tampon on sack, strain is accessed in cultivating bag rapidly, has changed tampon, has connect
During kind, strain bottleneck will will also be crossed, ensure at any time close to alcolhol burner burning things which may cause a fire disaster when tampon is pulled up on alcolhol burner burning things which may cause a fire disaster
Whole seeded process is not contaminated.
Inoculation it is noted that it is following some：
Carry out sterilization；To whole inoculation environment formalin, potassium permanganate carries out thorough disinfection；Inoculation is moved
Making will soon, to prevent miscellaneous bacteria from invading and infecting, it is necessary to continue tampon while being inoculated with, after the completion of inoculation, rapidly seal up tampon.
Flame disinfection must be concentrated rapidly, it is ensured that strain is advisable from alcolhol burner flame 6-8cm.
Pulverized limestone is sprinkled after inoculation on sack, in this way, can prevent germ from being infected at sack, then, moves on to and accompanies bacterium room
Carry out cultural hypha.
Bacterium germination
Bacterium germination band after inoculation will move into the bacterium germination room that temperature is 25 degrees Celsius in time, and restocking culture, cultivating bag is in frame
On want close-packed arrays, can keep the temperature of mycelial growth to produce, pay attention to that regulation temperature is 25 degrees centigrades, air is relatively wet
Spend for more than 70%, to prevent miscellaneous bacteria from breeding.During bacterium germination, a bacterium germination situation was checked every 3-5 days.If it find that culture medium
In have the spot of red, green, yellow, black color, illustrate that the sack being infected by bacteria should be detected in time by living contaminants.Bacterium germination
In the later stage, high temperature is prevented, temperature adjustment is to 22-24 degrees Celsius, and relative air humidity is within 75%.
Cultural hypha 85d, full bag is covered with, is reached maturity；Physiological maturity can be reached during cultural hypha 90d or so, at this moment
A bag fruiting can be taken off.
Do block fruiting
After hyphal development maturation, polybag is first sloughed, is carried out disinfection in liquor potassic permanganate, then slough old bacterium
Skin, thalline is rubbed with the hands broken, particle about 1cm sizes, then carries out doing block.The specification for doing block template die is chosen, long 40cm；Wide 30cm；It is high
8cm is advisable.The bacterium grain rubbed with the hands is poured into mould, is compacted, flattens, thin film is then sealed on cultivating stand, by ready-made fungus block
It is placed on cultivating stand, then removes template die, it is ensured that distance 4-5cm between block and block.After fungus block is put well, plastic sheeting is covered tightly,
Prevent moisture loss and miscellaneous bacteria from invading.To be managed with delicacy after doing block, using ventilation, hypha wound is healed rapidly, not by
Miscellaneous bacteria infects.Specific practice is that discarded, change fresh air is first discharged with film, about 6d or so, and mycelial growth is vigorous.
De- bag fruiting
Mycelia physiological maturity, can be with de- band fruiting.De- bag method, sack is opened, pushs plastic sheeting aside, takes off bag degree
Whole polybag can be sloughed depending on mycelia situation, if mycelium is expanded to whole bacterium bag；If only some
Mycelium expansion upheaval, the half of polybag can be only sloughed, treat that mycelia grows good and then sloughs remaining polybag, gradually
De- bag also has the effect for keeping humidity.Such as de- bag is too early, and mycelia is not reaching to physiological maturity；De- bag is too late, excessively ripe, can lead
Bag internal water accumulation is caused, causes mould contamination.During de- bag, temperature is no more than 25 degrees Celsius, with less than 22 degrees Celsius preferably.High temperature is high
It is wet easily to cause living contaminants, after taking off bag, plastic covering film, heat and moisture preserving.
Mycelia annesl
In this stage, mycelia slowly becomes pink by white, then becomes sepia, and strong light stimulus can accelerate annesl, temperature
Control is at 22-25 degrees Celsius, and air humidity is 85% or so.To strengthen daylight light irradiation after de- bag.
Management of producing mushroom
Creation that must be artificial after annesl is cold and hot, dry and wet and light and shade condition stimulate mycelia, make mycelia constantly break up with it is swollen
Change.Growth of Fruit-bodies In Lentinus Edodes maturation takes around the 7d times, and mushroom former base is formed and the temperature of fruiting body differentiation is Celsius in 10-20
Degree left and right, 15 degrees Celsius are optimal.Management of producing mushroom, to accomplish alternation of wetting and drying, caloric stimulation, keep the corresponding humidity of air 90%
Left and right.Present vaporific in film and have the globule, illustrate that humidity is suitable.Typically only need 3-4d from mushroom flower bud to fructification, need during low temperature
After wanting 6-7d, every batch of mushroom harvesting to terminate, mould is eliminated with knife, covers film.
A kind of implantation methods of mushroom provided in an embodiment of the present invention, it is easy to learn, and the production of mushroom can be significantly improved
Amount and quality, be high yield and volume production mushroom effective ways, highly popularization and application.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., it should be included in the present invention's
Within protection domain.
Claims (1)
1. a kind of implantation methods of mushroom, it is characterised in that comprise the following steps：Step 1, compost configuration；Mushroom one-level kind
Culture medium prescription be 250 parts of the potato of peeling, 25 parts of glucose, 20 parts of agar, clear water；Major ingredient is the two level kind of wood chip
Culture medium prescription, it is wood chip 78% according to mass fraction ratio, wheat bran 20%, land plaster 1%, sucrose 1%, material-water ratio 1:1.2；
Step 2, pack：Compost prepared by step 1 loads polyethylene plastic bag, and the drift angle of cultivating bag folds squarely,
Being fed again with charger, during charging, the hand entrusted is firmly uniform, make the compost compactness in bag consistent, if
Hand charging is, it is necessary to which while charging, while compacting, in material loading bag 3/5, cultivating bag requires, bag long 33-55cm, wide 17-
30cm, per packed 1-1.5 kilograms of siccative；
Step 3, sterilizing：When the temperature of bacterium bag is naturally cooled to below 25 degrees Celsius, can starts to be inoculated with；
Step 4, inoculation：Cultivating bag enters in inoculating hood along track, carries out ultraviolet light, under ultraviolet irradiation, first will
Inoculation bottle is opened, and removes the mycoderm on strain surface with inoculation cream, and digs up the old mycelia on strain upper strata, is then extracted on sack
Tampon, strain is accessed in cultivating bag rapidly, has changed tampon, in seeded process, strain bottleneck will at any time close to alcolhol burner burning things which may cause a fire disaster,
Also to be crossed on alcolhol burner burning things which may cause a fire disaster when tampon is pulled up, ensure that whole seeded process is not contaminated；
Step 5, bacterium germination：Bacterium germination band after inoculation will move into the bacterium germination room that temperature is 25 degrees Celsius in time, restocking culture, plant
Training bag wants close-packed arrays on frame, to produce the temperature that can keep mycelial growth, cultural hypha 85d, covers with full bag, develops into
It is ripe；
Step 6, take off bag processing：Sack is opened, pushs plastic sheeting aside, when taking off bag, temperature is kept no more than 25 degrees Celsius
Below 22 degrees Celsius；
Step 7, mycelia annesl：Mycelia slowly becomes pink by white, then becomes sepia, and strong light stimulus can accelerate annesl,
At 22-25 degrees Celsius, air humidity will strengthen daylight light irradiation after 85% or so, de- bag for temperature control.
Step 8, management of producing mushroom：Creation that must be artificial after annesl is cold and hot, dry and wet and light and shade condition stimulate mycelia, makes mycelia
Constantly break up and expanded, mushroom former base is formed and the temperature of fruiting body differentiation is at 10-20 degrees Celsius, keeps the corresponding humidity of air to exist
90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710591947.8A CN107493957A (en) | 2017-07-19 | 2017-07-19 | A kind of implantation methods of mushroom |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710591947.8A CN107493957A (en) | 2017-07-19 | 2017-07-19 | A kind of implantation methods of mushroom |
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| CN107493957A true CN107493957A (en) | 2017-12-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710591947.8A Pending CN107493957A (en) | 2017-07-19 | 2017-07-19 | A kind of implantation methods of mushroom |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108605656A (en) * | 2018-04-25 | 2018-10-02 | 习水县青野种植有限公司 | A kind of mushroom implantation methods |
| CN108617402A (en) * | 2018-04-24 | 2018-10-09 | 习水县青野种植有限公司 | A kind of method for cultivating mushroom |
| CN109315224A (en) * | 2018-11-29 | 2019-02-12 | 罗源县生产力促进中心 | Mushroom materials bag cultivating technique |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1943311A (en) * | 2006-10-24 | 2007-04-11 | 山西农业大学 | Method for cultivating edible mushroom and special tools |
| CN103749152A (en) * | 2013-12-31 | 2014-04-30 | 天津市金三农农业科技开发有限公司 | Shitake mushroom planting method |
| CN104396568A (en) * | 2014-12-03 | 2015-03-11 | 富顺县原野食用菌开发有限责任公司 | Cultivation technology of straw mushroom |
| CN105940952A (en) * | 2016-05-17 | 2016-09-21 | 旌德县兴农灵芝专业合作社 | Mushroom culture method |
-
2017
- 2017-07-19 CN CN201710591947.8A patent/CN107493957A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1943311A (en) * | 2006-10-24 | 2007-04-11 | 山西农业大学 | Method for cultivating edible mushroom and special tools |
| CN103749152A (en) * | 2013-12-31 | 2014-04-30 | 天津市金三农农业科技开发有限公司 | Shitake mushroom planting method |
| CN104396568A (en) * | 2014-12-03 | 2015-03-11 | 富顺县原野食用菌开发有限责任公司 | Cultivation technology of straw mushroom |
| CN105940952A (en) * | 2016-05-17 | 2016-09-21 | 旌德县兴农灵芝专业合作社 | Mushroom culture method |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108617402A (en) * | 2018-04-24 | 2018-10-09 | 习水县青野种植有限公司 | A kind of method for cultivating mushroom |
| CN108605656A (en) * | 2018-04-25 | 2018-10-02 | 习水县青野种植有限公司 | A kind of mushroom implantation methods |
| CN109315224A (en) * | 2018-11-29 | 2019-02-12 | 罗源县生产力促进中心 | Mushroom materials bag cultivating technique |
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Application publication date: 20171222 |