CN107493897A - The method grown with AMF and organic fertilizer promotion sugar grass in salt-soda soil - Google Patents
The method grown with AMF and organic fertilizer promotion sugar grass in salt-soda soil Download PDFInfo
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- CN107493897A CN107493897A CN201710797016.3A CN201710797016A CN107493897A CN 107493897 A CN107493897 A CN 107493897A CN 201710797016 A CN201710797016 A CN 201710797016A CN 107493897 A CN107493897 A CN 107493897A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The present invention relates to the method grown with AMF and organic fertilizer promotion sugar grass in salt-soda soil, in the seed of salt-soda soil sowing sugar grass, Arbuscular Mycorrhizal Fungi microbial inoculum and application of organic fertilizers simultaneously, conventional field management is carried out in sugar grass growth course until harvest.In the present invention organic fertilizer by adsorbing, being complexed, reducing, the effect such as volatilize increase the shock-absorbing capacity and adsorption capacity of soil, reduce salt ion toxic action, promote plant growth；AMF reduces the validity of salt ion by chelation, the effect of mycelia fixing etc., plant patience is improved by improving plant nutrient situation, changing the number of ways such as rhizosphere physicochemical character, change Root morphology, the expression of some resistant gene of salt of regulation and synthesis of GAP-associated protein GAP, AMF and organic fertilizer is administered simultaneously, alleviation Saline Alkali Stress can be cooperateed with.
Description
Technical field
The present invention relates to sugar grass implantation methods field, and in particular to one kind AMF and organic fertilizer promote sweet tea
The method that sorghum grows in salt-soda soil.
Background technology
Sugar grass is one of energy-source plant of China's focus development, because its Nutrient matter is high, therefore can be used as production
The quality raw materials of ethanol.Sugar grass has the energy such as stronger drought resisting, waterlogging, Salt And Alkali Tolerance, barren-resistant, high temperature resistant, dry heat resistance wind
Power, it is adapted in the area that some weather conditions are unfavorable, working condition is bad, such as arid, semiarid, low-lying easily flood and salt-soda soil,
Soil depletion mountain area and upper level plantation.Sugar grass is planted in non-agricultural land soil more.
China's saline alkali land area is larger, and because its salt content is higher, alkalescence is big, is unfavorable for the growth of crops, therefore salt
The utilization on alkali ground are urgent problems to be solved in agricultural production.Being plant sweet sorghum in salt-soda soil can not only accomplish " no
Ground is striven with grain ", and the utilization in salt-soda soil are also beneficial to, have for solving the problems such as China's energy shortage, arable land is insufficient
Significance.But sugar grass is sprouted in seed and the growth of seedling stage is poor to saline and alkaline patience, and salt-soda soil is general
The problems such as soil fertility is relatively low, soil physico-chemical property is poor, diversity of soil microorganism is few be present, this will certainly influence sweet tea height
The growth of fine strain of millet.
Numerous studies confirm that the improvement of organic fertilizer, bio-fertilizer for salt-soda soil can play a positive role.Organic fertilizer
Humus can be formed after microbial mineralization decomposes, and produces a large amount of organic acids, on the one hand can neutralize the alkali of salt affected soil
Property, it on the other hand can promote the conversion of nutrient.Application of organic fertilizers can increase organic matter content in soil, improve soil fertilizer
Power, while organic matter can play cushioning effect to salt ion, suppress harmful effect of the salt to plant.Microorganism such as fixed nitrogen
Bacterium, phosphobacteria, B. mucilaginocus, mycorrhizal fungi etc. can improve plant nutrient, provide plant stress-resistance performance, to the plant in salt-soda soil
Thing plays growth-promoting functions.It is published in《Agricultural University of the Inner Mongol's journal (natural science edition)》(2013,(6):102-109)《One
Influence of the kind microbial-bacterial fertilizer to sweet sorghum seed sprouting, growth of seedling and anti-adversity ability》Disclose bacillus amyloliquefacienses FZB42
The drought resistance and salt tolerance of seedling can be improved.It is published in《Acta Prataculture》(2014,23(6):274-278)《Sugar grass root
Border phosphorus-solubilizing bacteria phosphate solubilization and strain characteristic research》The phosphorus-solubilizing bacteria disclosed from the separation of sugar grass rhizosphere has phosphate solubilization and divided
IAA abilities are secreted, sugar grass can be promoted to grow after inoculation.It is published in《Chinese agronomy circular》(2017,33(16):12-18)
《Influence of the biological organic liquid mixed fertilizer to sugar grass root distinction coefficient》It was found that biological organic Liquid Fertilizer is beneficial to promote sweet tea high
Fine strain of millet Seedling root biomass accumulation and Root Absorption ability.The Chinese invention patent specification of Application No. 200810118837.0
Disclose it is a kind of be suitable for sugar grass bio-organic fertilizer special and the preparation method that salt-soda soil uses, this fertilizer is by 65-80%'s
Organic fertilizer, 5-10% streptomyces microflavus culture and 15-25% compound bacteria (containing a variety of bacillus and saccharomycete,
Lactic acid bacteria) culture is made；The Chinese invention patent specification of Application No. 200910077218.6 discloses one kind and is suitable for
The sugar grass special complex fertilizer and preparation method that salt-soda soil uses, this fertilizer is by urea, MAP, streptomyces microflavus culture
Thing, complex bacteria culture and amide-type high-molecular compound binding agent are made.
AMF is common in the ecosystems such as farmland, forest, grassland, in lean soil, salt-soda soil, colliery, gold
It is also distributed in the stressful environmentals such as category mining area, heavy-metal contaminated soil.According to statistics, in terrestrial ecosystems more than 80% plant
Thing can establish good mutualistic symbiosis system with AMF.Promote soil Mineral Elements, water from mycorrhizal fungi
Divide and transported to plant root so that plant absorption utilizes, and improve stress resistance of plant, improve soil texture, to degeneration ecosystem
The ecological recovery of system has positive role.
But it is related to AMF there has been no relevant report at present and organic fertilizer is shared in terms of sugar grass Salt And Alkali Tolerance
Effect.
The content of the invention
It is an object of the invention to provide a kind of method for promoting sugar grass to be grown in salt-soda soil, with AMF and have
Machine fertilizer significantly reduces the murder by poisoning of salt ion in salt-soda soil, and collaboration helps sugar grass to resist Saline Alkali Stress, improves sugar grass mineral battalion
Support, promote the growth of sugar grass.
In order to solve the above technical problems, the technical solution adopted in the present invention is：Promoted with AMF and organic fertilizer
Enter the method that sugar grass grows in salt-soda soil, the seed of sugar grass, while Arbuscular Mycorrhizal Fungi microbial inoculum are sowed in salt-soda soil
And application of organic fertilizers, conventional field management is carried out in sugar grass growth course until harvest.
Further, the content of organic matter >=45% in described organic fertilizer, amount of application is every mu of 130-400kg, using side
Method is to spread fertilizer over the fields or spread manuer in holes.
Further, described AMF is that Moses's pipe handle capsule is mould, Gen Neigensheng capsules are mould and Scotland pipe handle capsule
Any one in mould or two or more mixtures.
Further, the preparation method of the bush mycorrhizal fungi preparation is：(1) preparation method of single microbial inoculum：It is described
Single microbial inoculum be Moses's pipe handle capsule mould agent or Gen Neigensheng capsule mould agent or Scotland pipe handle capsule mould agent；To contain phase
Answer the AMF spore of strain, mycelia and infected the soil sample of plant root segment as initial inoculation agent, with agricultural land soil
Mixture with organic fertilizer is expands numerous matrix, using corn or sudangrass as host plant, using host plant after sterilization treatment
The numerous matrix of expansion in expand numerous AMF, expand it is numerous after obtain containing host plant root segment, AMF spore, root
The air-dried matrix of outer mycelia is single microbial inoculum, and fungal spore content >=10 in the single microbial inoculum/gram；(2) mix bacterium agent
Preparation method：The agent of Moses pipe handle capsule mould, Gen Neigensheng capsule mould agent and Soviet Union's lattice are prepared respectively according to method described in (1)
Two or more in blue pipe handle capsule mould agent are simultaneously well mixed, that is, mixing is made from mycorrhizal fungi microbial inoculum.
Further, the inoculum concentration of initial inoculation agent is matrix quality in the preparation method of the bush mycorrhizal fungi preparation
3%-5%, inoculation method excuted a law using layer or mixed excuted a law.
Further, expand the preparation of numerous matrix in the preparation method of the bush mycorrhizal fungi preparation and sterilizing methods are：
Agricultural land soil and organic fertilizer are air-dried respectively and cross 2mm sieves, after sieving, by agricultural land soil and organic fertilizer by volume 2:1 mixing
Uniformly, sterilized after being sterilized separately or mixing before agricultural land soil mixes with organic fertilizer, that is, matrix is made.
Further, the sterilizing methods for expanding numerous matrix in the preparation method of the bush mycorrhizal fungi preparation are：121 DEG C of steamings
Vapour sterilizes, and is taken out after the 1-2 hours that sterilize and is cooled to room temperature.
Further, the amount of application of the bush mycorrhizal fungi preparation is every mu of 300-600kg, the planting density of sugar grass
For every mu of 6000-9000 strain.
Further, during step 1 sowing host plant, water is added in matrix, the addition of water is matrix and initial inoculation
The 15%-20% of agent gross mass；Host plant carries out Routine Management to ensure it after planting and in growth course, to host plant
Normal growth, culture 3-4 months, cuts off the overground part of host plant, shreds root segment and is mixed with matrix, contains host plant
The air-dried matrix of the outer mycelia of root segment, AMF spore, root is single bush mycorrhizal fungi preparation.
Further, the bush mycorrhizal fungi preparation application process is that perforated method or layer are excuted a law or mixed and excute a law.
Beneficial effect：
1st, for the present invention by applying AMF and organic fertilizer, collaboration promotes plant growth, common to improve plant mineral battalion
Support, reduce saline and alkaline murder by poisoning；Wherein organic fertilizer is in addition to supplying nutrient, and also by adsorbing, being complexed, reducing, the effect such as volatilize increases
Add the shock-absorbing capacity and adsorption capacity of soil, reduce salt ion toxic action, promote plant growth；AMF can be with
The validity of salt ion is reduced by chelation, the effect of mycelia fixing etc., by improving plant nutrient situation, changing rhizosphere
Physicochemical character, change the number of ways raising plant such as Root morphology, the expression of some resistant gene of salt of regulation and synthesis of GAP-associated protein GAP
Thing patience, in of the invention, AMF and organic fertilizer is administered simultaneously, alleviation Saline Alkali Stress can be cooperateed with.
2nd, the organic fertilizer that the present invention is prepared in the microbial inoculum numerous matrix of expansion used can be to playing certain guarantor from mycorrhizal fungi
Shield acts on, and especially microbial inoculum is applied to the initial stage in salt-soda soil, and organic acid caused by its decomposition can be to around mycorrhizal fungi
Salt ion in soil plays cushioning effect, so as to help to resist Saline Alkali Stress from mycorrhizal fungi；
3rd, method simple practical of the invention, be easy to grasp, cost it is relatively low, green, secondary pollution will not be caused.
Embodiment
With reference to specific embodiment, the present invention will be further described in detail.
The method grown with AMF and organic fertilizer promotion sugar grass in salt-soda soil, sugar grass is sowed in salt-soda soil
Seed, while Arbuscular Mycorrhizal Fungi microbial inoculum and application of organic fertilizers carry out conventional field pipe in sugar grass growth course
Reason is until harvest.
Further, the content of organic matter >=45% in described organic fertilizer, amount of application is every mu of 130-400kg, using side
Method is to spread fertilizer over the fields or spread manuer in holes.
Further, described AMF is that Moses's pipe handle capsule is mould, Gen Neigensheng capsules are mould and Scotland pipe handle capsule
Any one in mould or two or more mixtures.
Further, the preparation method of the bush mycorrhizal fungi preparation is：(1) preparation method of single microbial inoculum：It is described
Single microbial inoculum be Moses's pipe handle capsule mould agent or Gen Neigensheng capsule mould agent or Scotland pipe handle capsule mould agent；With containing corresponding
The AMF spore of strain, mycelia and infected the soil sample of plant root segment as initial inoculation agent, with agricultural land soil with
The mixture of organic fertilizer is expands numerous matrix, using corn or sudangrass as host plant, using host plant after sterilization treatment
Expand in numerous matrix and expand numerous AMF, obtained after expansion is numerous outside containing host plant root segment, AMF spore, root
The air-dried matrix of mycelia is single microbial inoculum, and fungal spore content >=10 in the single microbial inoculum/gram；(2) mix bacterium agent
Preparation method：The agent of Moses pipe handle capsule mould, Gen Neigensheng capsule mould agent and Scotland are prepared respectively according to method described in (1)
Two or more in pipe handle capsule mould agent are simultaneously well mixed, that is, mixing is made from mycorrhizal fungi microbial inoculum.
Further, the inoculum concentration of initial inoculation agent is matrix quality in the preparation method of the bush mycorrhizal fungi preparation
3%-5%, inoculation method excuted a law using layer or mixed excuted a law.
Further, expand the preparation of numerous matrix in the preparation method of the bush mycorrhizal fungi preparation and sterilizing methods are：
Agricultural land soil and organic fertilizer are air-dried respectively and cross 2mm sieves, after sieving, by agricultural land soil and organic fertilizer by volume 2:1 mixing
Uniformly, sterilized after being sterilized separately or mixing before agricultural land soil mixes with organic fertilizer, that is, matrix is made.
Further, the sterilizing methods for expanding numerous matrix in the preparation method of the bush mycorrhizal fungi preparation are：121 DEG C of steamings
Vapour sterilizes, and is taken out after the 1-2 hours that sterilize and is cooled to room temperature.
Further, the amount of application of the bush mycorrhizal fungi preparation is every mu of 300-600kg, the planting density of sugar grass
For every mu of 6000-9000 strain.
Further, during step 1 sowing host plant, water is added in matrix, the addition of water is matrix and initial inoculation
The 15%-20% of agent gross mass；Host plant carries out Routine Management to ensure it after planting and in growth course, to host plant
Normal growth, culture 3-4 months, cuts off the overground part of host plant, shreds root segment and is mixed with matrix, contains host plant
The air-dried matrix of the outer mycelia of root segment, AMF spore, root is single bush mycorrhizal fungi preparation.
Further, the bush mycorrhizal fungi preparation application process is that perforated method or layer are excuted a law or mixed and excute a law.
Embodiment 1
Using the mould method for promoting sugar grass to be grown in salt-soda soil of Moses's pipe handle capsule, mould described Moses's pipe handle capsule is Moses's pipe handle
Capsule is mould (Funneliformis mosseae), and the strain is common strain, is provided by China Agricultural University, methods described includes
Following steps：
Step 1: prepare Moses's pipe handle capsule mould agent
Initial inoculation agent is containing Moses's pipe handle capsule mould (Funneliformis mosseae) spore, mycelia and is infected root segment
Soil sample；
Agricultural land soil and organic fertilizer are air-dried respectively, by 2mm fine screen mesh sieving, 121 DEG C of high pressure steam sterilizations 1 hour, it is high
Taken out after pressure steam sterilizing and be cooled to room temperature, then the agricultural land soil and organic fertilizer for being cooled to room temperature according to volume ratio 2:1 mixing
Stir to form matrix；
Described initial inoculation agent is inoculated with matrix using mixed excute a law, inoculum concentration is the 5% of matrix quality, then in matrix
Adding running water, the addition of running water is the 15% of matrix and initial inoculation agent gross mass, the seeding corn seed in matrix,
Corn every 3 weeks Huo Gelan that 1/4 intensity is poured according to the 10% of matrix and initial inoculation agent gross mass in growth course
(Hoagland) nutrient solution 1 time, this nutrient solution whole nutrients necessary to include plant growth, it is that culture plant is commonly used
Nutrient solution；Incubation is carried out in heliogreenhouse, pays attention to carrying out the management such as moisture, disease, it is ensured that plant normal growth；
Growth can obtain by the mould corn infected of Moses's pipe handle capsule after 4 months, by by the mould corn infected of Moses's pipe handle capsule
Aerial part is cut off, and corn root segment is shredded and mixed with the matrix containing spore, can be obtained containing corn root after air-drying
Section, the fungal inoculant of the outer mycelia of the mould spore of Moses's pipe handle capsule, root, fuugal spores counts contained by the fungal inoculant should >=10/
Gram, if contained/gram of fuugal spores counts ＜ 10, can by extend harvest time or sowed again in matrix host plant with
Expand the brood body of AMF, when especially temperature is low in the winter time, expand numerous process can in the controlled environment chamber in enter
OK；
Step 2: Moses's pipe handle capsule mould agent and the administration of organic fertilizer
Test site：The farmland in Quan Tou villages of Henan Province Kaifeng Lankao County Yi Feng townshiies, its preceding crop are wheat, and soil is slight
Salinization of soil, wheat normal growth is not influenceed；Organic fertilizer is bought in Luoyang Wo Nong agricultural science and technologys Co., Ltd, and the content of organic matter >=
45%；Sugar grass kind is refined Tianjin 2, and in the market buys gained；
Plot area is 4m × 7.5m=30m2, per the row of cell kind 8, line-spacing 0.5m often goes long 7.5m, and spacing in the rows is respectively 15cm,
Often 50 plants of row；Each cell density is 400 plants；Aisle 1-2m is stayed between cell；
Conventional arrangement before being sowed before sowing to plot, is adapted to plant, after sowing works in the rain of in early June, 2016
Carry out, therefore need not water within 2 days, every bunch planting kind sweet sorghum seed 5；Inoculation processing is often spreaded manuer in holes plus 50 grams of Moses's pipe handle capsules
Mould agent, is overlying on above seed, blinding；Inoculation processing includes applying organic fertilizer processing respectively and does not apply organic fertilizer, is applied with
The processing of machine fertilizer is not inoculated with control treatment according to every cell 6.6kg (closing about 141 kilograms every mu, per hectare 2200kg) and not applied
From mycorrhizal fungi microbial inoculum；Each processing is repeated 4 times；
The high laggard seedlings in the ranks of sugar grass emergence 10cm, seedling 1 is stayed per cave；Growth period optionally carries out weeding, watering, anti-bird etc.
Processing, ensure sugar grass normal growth；Early October harvests stalk, root system and seed, measure life respectively after sugar grass maturation
Produce amount, its measurement result such as table 1：
The influence of the Moses's pipe handle capsule mould agent of table 1 and organic fertilizer to sugar grass (refined Tianjin 2) stalk fresh weight and grain yield
| Processing | Stalk fresh weight (kg/hm2) | Grain yield (kg/hm2) |
| Compare (not applying microbial inoculum and organic fertilizer) | 59800±234 | 12400±202 |
| Using Moses's pipe handle capsule mould agent | 65520±320 | 13100±220 |
| Application of organic fertilizers | 66600±542 | 13600±320 |
| Using Moses's pipe handle capsule mould agent and organic fertilizer | 68000±485 | 13900±356 |
As can be seen from Table 1, the stalk mean fresh of control treatment is 59800kg/hm2, using the agent of Moses pipe handle capsule mould, apply
It is respectively 65520kg/hm with organic fertilizer, the processing stalk mean fresh for being administered simultaneously microbial inoculum and organic fertilizer2、66600kg/hm2、
68000kg/hm2, increase by 9.6%, 11.4%, 13.7% respectively than control treatment；Control treatment seed average product is
12400kg/hm2, put down using the agent of Moses pipe handle capsule mould, application of organic fertilizers, the processing seed that is administered simultaneously microbial inoculum and organic fertilizer
Equal yield is respectively 13100kg/hm2、13600kg/hm2、13900kg/hm2, add 5.6% respectively than control treatment,
9.7%th, 12.1%；It can be seen that it can promote growth of the sugar grass in salt-soda soil, and bacterium with organic fertilizer using Moses's pipe handle capsule is mould
Both agent and organic fertilizer share promotes sugar grass in the effect of the growth in salt-soda soil with collaboration.
Embodiment 2
Using the mould method for promoting sugar grass to be grown in salt-soda soil of Gen Neigensheng capsules, except AMF strain and sugar grass product
Outside kind and the difference of embodiment 1, remaining all same；
AMF is that Gen Neigensheng capsules are mould (Rhizophagus intraradices), is provided by China Agricultural University；
Sugar grass kind is distant sweet tea 5, and the sugar grass kind buys gained by market；The measure knot of biological yield after sugar grass maturation
Fruit such as table 2：
The influence of the Gen Neigensheng capsule mould agent of table 2 and organic fertilizer to sugar grass (distant sweet tea 5) stalk fresh weight and grain yield
| Processing | Stalk fresh weight (kg/hm2) | Grain yield (kg/hm2) |
| Compare (not applying microbial inoculum and organic fertilizer) | 62200±332 | 13200±197 |
| Using Gen Neigensheng capsule mould agent | 68500±357 | 13800±261 |
| Application of organic fertilizers | 68600±464 | 14100±337 |
| Using Gen Neigensheng capsule mould agent and organic fertilizer | 72100±513 | 14500±378 |
As can be seen from Table 2, the stalk mean fresh of control treatment is 62200kg/hm2, using the agent of Gen Neigensheng capsule moulds, apply
It is 68500kg/hm with organic fertilizer, the processing stalk average organism yield for being administered simultaneously microbial inoculum and organic fertilizer2、68600kg/hm2、
72100kg/hm2, increase by 10.1%, 10.3%, 15.9% respectively than control treatment；Control treatment seed average product is
13200kg/hm2, put down using the agent of Gen Neigensheng capsule moulds, application of organic fertilizers, the processing seed that is administered simultaneously microbial inoculum and organic fertilizer
Equal yield is respectively 13800kg/hm2、14100kg/hm2、14500kg/hm2, increase by 4.5% respectively than control treatment,
6.8%th, 9.8%；It can be seen that using Gen Neigensheng capsules are mould and organic fertilizer can promote growth of the sugar grass in salt-soda soil, and microbial inoculum and
Both organic fertilizers share promotes sugar grass in the effect of the growth in salt-soda soil with collaboration.
Embodiment 3
Using the mould method for promoting sugar grass to be grown in salt-soda soil of Scotland pipe handle capsule, except AMF strain and embodiment
1 is different and employ outside two sugar grass kinds, remaining all same；
AMF is that Scotland pipe handle capsule is mould (Funneliformis caledonium), by Chinese Academy of Sciences Nanjing soil
Earth research institute provides；Sugar grass kind is refined Tianjin 2 and distant sweet tea 5, is market purchase gained；It is biological after sugar grass maturation
The measurement result of yield such as table 3：
Scotland pipe handle capsule mould agent of table 3 and organic fertilizer produce to sugar grass (refined Tianjin 2 and distant sweet tea 5) stalk fresh weight and seed
The influence of amount
As can be seen from Table 3, for refined No. 2 sugar grasses in Tianjin, using Scotland pipe handle capsule is mould, application of organic fertilizers, bacterium is administered simultaneously
The processing stalk mean fresh of agent and organic fertilizer is respectively 62800kg/hm2、66600kg/hm2、67800kg/hm2, than control
Increase by 5.0%, 11.4%, 13.4% respectively；For distant No. 5 sugar grasses of sweet tea, put down using the mould processing stalk of Scotland pipe handle capsule
Equal fresh weight is 65800kg/hm2、68600kg/hm2、71500kg/hm2, increase by 5.8% respectively than control treatment, 10.3%,
15.0%；It can be seen that Scotland mould stalk mean fresh to two kinds of sugar grasses of pipe handle capsule has effect of increasing production, but effect is not as rubbing
Western pipe handle capsule is mould and Gen Neigensheng capsule mould agent.
Embodiment 4
The method grown using mixing from mycorrhizal fungi microbial inoculum promotion sugar grass in salt-soda soil, is comprised the following steps：
Step 1: mixing is prepared from mycorrhizal fungi microbial inoculum
The agent of Moses pipe handle capsule mould, Gen Neigensheng capsule mould agent and Scotland pipe handle capsule that are prepared respectively in embodiment 1-3 is mould
Microbial inoculum, which is pressed, waits mass ratio to mix, you can obtains mixing from mycorrhizal fungi microbial inoculum；
Step 2: administration of the mixing from mycorrhizal fungi microbial inoculum
Application process is the same as embodiment 1；Sugar grass kind is the same as embodiment 3；The measurement result of biological yield such as table after sugar grass maturation
4：
Table 4 is mixed from mycorrhizal fungi microbial inoculum and organic fertilizer to sugar grass (refined Tianjin 2 and distant sweet tea 5) stalk fresh weight and seed
The influence of yield
As can be seen from Table 4, for refined No. 2 sugar grasses in Tianjin, using mixing bush mycorrhizal fungi preparation, application of organic fertilizers, simultaneously
Processing stalk mean fresh using microbial inoculum and organic fertilizer is 67100kg/hm2、66600kg/hm2、68800kg/hm2, compare
Increase by 12.2%, 11.4%, 15.1% respectively according to processing；For distant No. 5 sugar grasses of sweet tea, using mixing AMF bacterium
Agent, application of organic fertilizers, microbial inoculum and the processing stalk mean fresh of organic fertilizer is administered simultaneously is 70100kg/hm2、68600kg/
hm2、 72700kg/hm2, increase by 12.7%, 10.3%, 16.9% respectively than compareing；, it is seen that mixing is utilized from mycorrhizal fungi
Microbial inoculum and organic fertilizer can promote growth of the sugar grass in salt-soda soil, and mix and closed from mycorrhizal fungi microbial inoculum and organic fertilizer
With with collaboration promote sugar grass the growth in salt-soda soil effect, and facilitation be better than three kinds of microbial inoculums individually with it is organic
The effect that fertilizer shares.
Above example is only the basic conception and the explanation made of embodiment to the present invention, it is impossible to is interpreted as pair
The limitation of the present invention, any modification and replacement not departed from the case of basic idea of the present invention, the protection of the present invention all should be included
Scope.
Claims (10)
1. the method grown with AMF and organic fertilizer promotion sugar grass in salt-soda soil, it is characterised in that：In salt-soda soil
The seed of sugar grass, while Arbuscular Mycorrhizal Fungi microbial inoculum and application of organic fertilizers are sowed, is carried out in sugar grass growth course
Conventional field management is until harvest.
2. the method according to claim 1 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
It is characterized in that：The content of organic matter >=45% in described organic fertilizer, amount of application are every mu of 130-400kg, and application process is to spread fertilizer over the fields
Or spread manuer in holes.
3. the method according to claim 1 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
It is characterized in that：Described AMF be that Moses's pipe handle capsule is mould, Gen Neigensheng capsules are mould and Scotland pipe handle capsule it is mould in
Any one or two or more mixtures.
4. the method according to claim 3 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
Characterized in that, the preparation method of the bush mycorrhizal fungi preparation is：
（1）The preparation method of single microbial inoculum：Described single microbial inoculum is Moses's pipe handle capsule mould agent or Gen Neigensheng capsule mould agent
Or Scotland pipe handle capsule mould agent；With the AMF spore containing corresponding strain, mycelia and infected plant root segment
Soil sample is as initial inoculation agent, using the mixture of agricultural land soil and organic fertilizer as numerous matrix is expanded, using corn or sudangrass as host
Plant, using numerous AMF is expanded in the numerous matrix of expansion of the host plant after sterilization treatment, obtained after expansion is numerous containing host
The air-dried matrix of the outer mycelia of plant root segment, AMF spore, root is single microbial inoculum, allergenic in the single microbial inoculum
Sub- content >=10/gram；
（2）The preparation method of mix bacterium agent：According to（1）Described in method prepare the agent of Moses pipe handle capsule mould, Gen Neigensheng respectively
Two or more in the agent of capsule mould and Scotland pipe handle capsule mould agent are simultaneously well mixed, that is, mixing is made from mycorrhizal fungi bacterium
Agent.
5. the method according to claim 4 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
It is characterized in that：The inoculum concentration of initial inoculation agent is the 3%- of matrix quality in the preparation method of the bush mycorrhizal fungi preparation
5%, inoculation method is excuted a law or mixed using layer and excuted a law.
6. the method according to claim 4 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
Characterized in that, expanding the preparation of numerous matrix in the preparation method of the bush mycorrhizal fungi preparation and sterilizing methods are：By farmland
Soil and organic fertilizer air-dry and cross 2mm sieves respectively, after sieving, by agricultural land soil and organic fertilizer by volume 2:1 is well mixed,
Agricultural land soil sterilizes after being sterilized separately or mix before being mixed with organic fertilizer, that is, matrix is made.
7. the method according to claim 4 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
Characterized in that, the sterilizing methods for expanding numerous matrix in the preparation method of the bush mycorrhizal fungi preparation are：121 DEG C of steam go out
Bacterium, taken out after the 1-2 hours that sterilize and be cooled to room temperature.
8. the method according to claim 4 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
It is characterized in that：The amount of application of the bush mycorrhizal fungi preparation is every mu of 300-600kg, and the planting density of sugar grass is every mu
6000-9000 strains.
9. the method according to claim 4 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
It is characterized in that：When step 1 sows host plant, water is added in matrix, the addition of water is matrix and the total matter of initial inoculation agent
The 15%-20% of amount；Host plant carries out Routine Management to host plant to ensure that it is normal raw after planting and in growth course
It is long, culture 3-4 months, the overground part of host plant is cut off, shred root segment and mixed with matrix, contain host plant root segment, clump
The air-dried matrix of the outer mycelia of mycorrhizal fungi spore, root is single bush mycorrhizal fungi preparation.
10. the method according to claim 1 for promoting sugar grass to be grown in salt-soda soil with AMF and organic fertilizer,
It is characterized in that：The bush mycorrhizal fungi preparation application process is that perforated method or layer are excuted a law or mixed and excute a law.
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