CN107488620A - A kind of cadmium pollution paddy soil microorganism passivator - Google Patents
A kind of cadmium pollution paddy soil microorganism passivator Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
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Abstract
The present invention relates to technological field of biochemistry.Purpose is to provide a kind of cadmium pollution paddy soil microorganism passivator.The technical scheme of use：A kind of microorganism passivator of cadmium pollution paddy soil and application, including following component：Pseudomonas alcaligenes, bacillus subtilis, sulfate reducing bacteria, nutriment and carrier.In described microorganism, Pseudomonas alcaligenes and bacillus subtilis mainly by direct or indirect Adsorption of Cadmium to antimicrobial surface or it is intracellular in solidified, so as to reduce the migration of cadmium and biological effectiveness, and Pseudomonas alcaligenes plays the role of to adjust soil pH.In described microorganism, sulfate reducing bacteria is mainly sulphion by sulphate reducing, forms cadmium sulfide with cadmium, cadmium is solidified in soil.The present invention can be passivated the cadmium in soil, significantly eliminate absorption of the rice to cadmium ion by the application to the microorganism passivator.This technology application, cost is low, easy to operate, has good application value.
Description
Technical field
The present invention relates to technological field of biochemistry, and in particular to a kind of microorganism passivator for administering cadmium pollution soil,
And its application in the solidification and passivation of paddy soil cadmium.
Background technology
Rice is the important cereal crops in China.Recent years, cadmium is in smelting, chemical industry and electronics industry etc. by extensive
Using this causes Cadmium in Soil (Cd) pollution to be on the rise.Paddy soil Cd pollution problem how is effectively prevented and treated and solved, is tieed up
The health and safety production of soil is held, is increasingly paid attention to by China.
Cd is the extremely strong heavy metal element of bio-toxicity, and it knows from experience to form biological accumulation into people, damage liver, kidney, brain with
And bone etc..United Nations Environment Programme and the international labour health heavy metal committee are set to priority pollutant；U.S.'s poison
Thing administration committee is classified as the 6th noxious material for jeopardizing human health.
Rice is the main cereal crops of our people.Because rice is planted in water logging soil mostly, Cd is at this
Migration is higher under part, therefore is easily absorbed by crops, and is accumulated in its seed, and then seriously jeopardizes health.How
The cadmium pollution in paddy soil is effectively handled, reduces enrichment of the cadmium in rice, it has also become China's agricultural and environment in recent years
The outstanding problem in protection field.
Pseudomonas is gramnegative bacterium, widely distributed in soil, sludge, there is stronger adaptation to environment
Property.Research shows：Pseudomonad, can be more by secreting Extracellular polymers and the glue of metal ion formation as biological adsorption agent
The metal complex of sugar, or exchanged by the covalent ion on polysaccharide with metallic divalent cations, and adsorbed on bacterial strain
The heavy metal ion such as precipitation, its adsorbable Pb (II), Co (II), Ni (II), Cu (II), Cd (II), Ti (IV).Some false unit cells
Bacterium also has extremely strong reducing power, such as Pseudomonas alcaligenes, can will be reduced the heavy metal ion of high-valence state by reduction
For lower valency, even elemental, so as to reduce the toxicity of heavy metal and transportable property.
Bacillus is Gram-positive bacillus, in distributed in nature than wide, there is stronger resist to poor environment
Property.Bacillus can be used as biological adsorption agent, adsorb heavy metal Pb2+And Cd2+.The researchs such as discipline magnificence are found：Bacillus subtilis
Soil available cadmium content can be made to reduce by 5.07%~42.63%, and incubation time is longer, its passivation effect is more notable.Model
Zhong Xue etc. is shown by pot experiment, best to the effect for alleviating peanut Cd stress using 2g/kg bacillus subtilis, can be made
Cd contents reduce by 25.37% in seed.Bacillus passivation Cd cardinal principle is that the cell membrane of bacillus is by peptide glycan
The condensate composition being formed by connecting with LTA.Peptide glycan is that -acetylmuramic acid 2-Acetamido-2-deoxy-D-glucose is alternate high poly-
Thing, these high polymers are in juxtaposition and are crosslinked, so as to form adsorption layer.In addition, bacillus cell wall is another
Individual structure is LTA, is made up of the glycerophosphate of short chain, has substantial amounts of negative electrical charge, an adsorbable positively charged huge sum of money
Category.
Sulfate reducing bacteria (Sulfate-Reducing Bacteria, abbreviation SRB) is a kind of microorganism of anaerobism, extensively
It is general to be present in the anaerobic environments such as soil, seawater, river, underground piping and Oil/gas Well.SRB can be by the SO in environment4 2-Turn
Turn to S2-, utilize S2-Sulfide precipitation is formed with the precipitation of metal (e.g., Fe, Cd) etc..SRB is widely used to handle
Heavy metal polluted waste water, but using less in heavy metal pollution of soil processing.
Rice Cropping often uses water logging soil, easily forms anaerobism microenvironment, therefore be adapted to SRB growth and breedings.Pseudomonad
Category and bacillus are facultative anaerobic bacteria, can also adapt to the environment of water logging soil.In addition, there is investigation to show south China
Many paddy soil slant acidities (4.6-5.2), and Cd ions are more easy to migrate in acid condition, bioactivity is high.And produce alkali vacation
Monad can also utilize Hydrogen Proton in growth course, and discharge hydroxide ion, raise pH value of solution, to improveing acid soil
Earth environment has certain effect, while can also reduce Cd biological effectiveness.
Microorganism passivator solidification paddy soil Cd have the advantages that to implement compared with it is simple, invest it is less, small to environmental disruption,
It is worth researching and developing energetically.
The content of the invention
It is an object of the invention to provide a kind of cadmium pollution paddy soil microorganism passivator, the passivator can make paddy soil
In cadmium passivation, significantly eliminate absorption of the rice to cadmium ion.
For achieving the above object, the technical solution adopted in the present invention is：A kind of cadmium pollution paddy soil microorganism
Passivator, including following component：Pseudomonas alcaligenes, bacillus subtilis, sulfate reducing bacteria and carrier.
Preferably：The microorganism passivator of described cadmium pollution paddy soil, each component weight composition are as follows：It is false single to produce alkali
Born of the same parents bacterium 30-40 parts, bacillus subtilis 20-30 parts, sulfate reducing bacteria 20-30 parts, carrier 30 as one kind 0-400 parts.
Preferably：The microorganism passivator of described cadmium pollution paddy soil, microorganism growth institute is added in the preparation
The nutriment 10-15 parts needed.
Preferably：The microorganism passivator of described cadmium pollution paddy soil, the carrier are biological powdered carbon, fineness 60-
80 mesh.
Preferably：The microorganism passivator of described cadmium pollution paddy soil, the Pseudomonas alcaligenes are DSM-50342
Or DSM-19550, bacillus subtilis are DSM-100605 or DSM-8565 or DSM-2109, sulfate reducing bacteria are desulfurization intestines
Shape bacterium DSM574 or desulfovibrio desulfurican DSM642.
Preferably：The microorganism passivator of described cadmium pollution paddy soil, effective bacterium of Pseudomonas alcaligenes used
Number is 1.0-2.0 × 109Cfu/g, effective bacterium number of bacillus subtilis used is 3.0-5.0 × 109Cfu/g, sulphur used
Effective bacterium number of hydrochlorate reducing bacteria is 3.0-5.0 × 108cfu/g。
Preferably：Described Pseudomonas alcaligenes, bacillus subtilis, sulfate reducing bacteria grow training with the following method
Obtained after supporting：Original strain is aseptically carried out to inclined-plane culture, shaking table seed culture, fermentation tank successively respectively and expands training
After supporting, obtained zymotic fluid is obtained into the culture bacterium powder by concentrate drying；Each different phase is used during three kinds of bacterium cultures
Culture medium it is identical.
Preferably：The composition of nutritive substance is dipotassium hydrogen phosphate (K2HPO4), sal-ammoniac (NH4CL), magnesium sulfate
(MgSO4), sodium lactate, dusty yeast and ferrous sulfate (FeSO4), its part by weight is：0.5：1.0：2.0：2.0：5.0：5.0.
Preferably：The microorganism passivator of described cadmium pollution paddy soil, the carrier are by straw or palm
The biological powdered carbon of shell or wooden class prepared by after high-temperature firing, dried and screened, fineness 60-80 mesh.
Preferably：The microorganism passivator of described cadmium pollution paddy soil, the carrier be palm shell through high-temperature firing,
Prepared biological powdered carbon, fineness 60-80 mesh after dried and screened.
Preferably：The microorganism passivator is powdery or graininess, and thin loess or incorporation base manure are mixed in ploughing soil
In uniformly spread fertilizer over the fields, level land, general dosage is 100-200kg/ mus.
The invention has the advantages that：Microorganism of the present invention can grow rapidly numerous in paddy soil
Grow, can by directly adsorbing, redox and form the Cd ions that the diversified forms such as sulfide precipitation are fixed in paddy soil, it is right
Cd is fixed and is passivated, and so as to reduce the biological effectiveness of Cd ions, cuts down absorption of the crop to Cd.The present invention is in microorganism
Some nutriments and biological powdered carbon necessary to microorganism growth is added in passivator, may advantageously facilitate the growth of microorganism
Breeding and colonizing in soil, improve the stability of passivator, solve because microbial activity is easily by various envirment factors
Influence (pH, nutrient, oxygen, moisture etc.), the problem of so as to influence its fixation and passivation effect to Cd.Such as use stalk
The crude fibres such as crops charcoal, then have the shortcomings that volume is larger, be not easy uniformly to mix with microorganism；And what the present invention used
It is the commercialization biology powdered carbon after high-temperature firing, drying, sieving, surface area is big, absorption is too strong, be well mixed with microorganism,
It is easy to operate, and powdered carbon also has certain heavy metal adsorption in itself after firing, positive effect is better than the letter of the crops such as stalk
Manufactured raw material after single crushing.In addition, the nutritional ingredient that the present invention is added can provide energy for microorganism amount reproduction.This
Invent that easy to implement, cost is low, there is good application value.
Embodiment
Embodiment one
The culture of Pseudomonas alcaligenes
Strain：Pseudomonas alcaligenes (DSM-50342 or DSM-19550).
Culture medium：Dusty yeast 5.0g, KNO31.0g, KH2PO41.0g, MgSO4·7H2O 1.0g, FeCl3·6H2O
0.05g, CaCl2·2H2O 0.2g, trisodium citrate 5.0g, distilled water 1000mL, pH 7.2-7.4.
Culture：Slant strains are inoculated with 2 rings in 100ml fluid nutrient mediums, are placed in 30 DEG C, 120rpm shaking table shaken cultivations
2-3 days, then by 10% inoculum concentration be transferred in fresh culture expand culture 48 hours, effective bacterium number be 1.0-2.0 ×
109cfu/g。
Embodiment two
The culture of bacillus subtilis
Strain：Bacillus subtilis (DSM-100605, or DSM-8565, or DSM-2109)
Culture medium：Peptone 5g, beef extract 3.0g, distilled water 1000mL, pH 7.0-7.2.
Culture：Slant strains are inoculated with 2 rings in 100ml fluid nutrient mediums, are placed in 30 DEG C, 120rpm shaking table shaken cultivations
2-3 days, then by 10% inoculum concentration be transferred in fresh culture expand culture 48 hours, effective bacterium number be 3.0-5.0 ×
109cfu/g。
Embodiment three
The culture of sulfate reducing bacteria
Sulfate reducing bacteria strain：Desulfotomaculum (DSM574) or desulfovibrio desulfurican (DSM 642), or its mixing
Thing；
Culture medium：KNO31.0g, K2HPO40.5g, NH4CL 1.0, Na2SO41.0, CaCL2.2H2O 0.1,MgSO4·
7H2O 2.0g, sodium lactate 2.0g, yeast extract 1.0g, FeSO4·7H2O 0.5g, distilled water 1000mL, pH 7.6-7.8.
Culture：Slant strains are inoculated with 2 rings in 100ml fluid nutrient mediums, are placed in 37-40 DEG C, the vibration training of 50rpm shaking tables
Support 7 days, then by 10% inoculum concentration be transferred in fresh culture expand culture 7-9 days, effective bacterium number be 3.0-5.0 ×
108cfu/g。
Example IV
A kind of cadmium pollution soil microorganism renovation agent, it includes following component：
20 parts of Pseudomonas alcaligenes, 20 parts of bacillus subtilis, 30 parts of sulfate reducing bacteria, 10 parts of nutriment, biology
300 parts of charcoal.
The Pseudomonas alcaligenes is preserving number DSM-50342 bacterial strain；
The bacillus subtilis is preserving number DSM-100605 bacterial strain；
The sulfate reducing bacteria is Desulfotomaculum (DSM574)；
Effective bacterium number of the Pseudomonas alcaligenes is 2.0 × 109Cfu/g, effective bacterium number of the bacillus subtilis
For 5.0 × 109Cfu/g, effective bacterium number of the sulfate reducing bacteria is 3.0 × 108cfu/g。
Nutriment dipotassium hydrogen phosphate (the K2HPO4), ammonium chloride (NH4CL), magnesium sulfate (MgSO4), sodium lactate and sulphur
Sour ferrous iron (FeSO4), its part by weight is：0.5：1.0：2.0：2.0：5.0.
Embodiment five
A kind of cadmium pollution soil microorganism renovation agent, it includes following component：
30 parts of Pseudomonas alcaligenes, 10 parts of bacillus subtilis, 50 parts of sulfate reducing bacteria, 20 parts of nutriment, biology
400 parts of charcoal.
The Pseudomonas alcaligenes is preserving number DSM-50342 bacterial strain；
The bacillus subtilis is preserving number DSM-100605 bacterial strain；
The sulfate reducing bacteria is Desulfotomaculum (DSM574)；
Effective bacterium number of the Pseudomonas alcaligenes is 2.0 × 109Cfu/g, effective bacterium number of the bacillus subtilis
For 5.0 × 109Cfu/g, effective bacterium number of the sulfate reducing bacteria is 3.0 × 108cfu/g。
Nutriment dipotassium hydrogen phosphate (the K2HPO4), ammonium chloride (NH4CL), magnesium sulfate (MgSO4), sodium lactate and sulphur
Sour ferrous iron (FeSO4), its part by weight is：0.5：1.0：2.0：2.0：5.0.
Embodiment six
A kind of cadmium pollution soil microorganism renovation agent, it includes following component：
20 parts of Pseudomonas alcaligenes, 40 parts of bacillus subtilis, 40 parts of sulfate reducing bacteria, 20 parts of nutriment, biology
300 parts of charcoal.
The Pseudomonas alcaligenes is preserving number DSM-50342 bacterial strain；
The bacillus subtilis is preserving number DSM-100605 bacterial strain；
The sulfate reducing bacteria is Desulfotomaculum (DSM574)；
Effective bacterium number of the Pseudomonas alcaligenes is 2.0 × 109Cfu/g, effective bacterium number of the bacillus subtilis
For 5.0 × 109Cfu/g, effective bacterium number of the sulfate reducing bacteria is 3.0 × 108cfu/g。
Nutriment dipotassium hydrogen phosphate (the K2HPO4), ammonium chloride (NH4CL), magnesium sulfate (MgSO4), sodium lactate and sulphur
Sour ferrous iron (FeSO4), its part by weight is：0.5：1.0：2.0：2.0：5.0.
Embodiment seven
A kind of cadmium pollution soil microorganism renovation agent, it includes following component：
20 parts of Pseudomonas alcaligenes, 20 parts of bacillus subtilis, 30 parts of sulfate reducing bacteria, 10 parts of nutriment, biology
300 parts of charcoal.
The Pseudomonas alcaligenes is preserving number DSM-19550 bacterial strain；
The bacillus subtilis is preserving number DSM-8565 bacterial strain；
The sulfate reducing bacteria is Desulfotomaculum (DSM 642)；
Effective bacterium number of the Pseudomonas alcaligenes is 2.0 × 109Cfu/g, effective bacterium number of the bacillus subtilis
For 5.0 × 109Cfu/g, effective bacterium number of the sulfate reducing bacteria is 3.0 × 108cfu/g。
Nutriment dipotassium hydrogen phosphate (the K2HPO4), ammonium chloride (NH4CL), magnesium sulfate (MgSO4), sodium lactate and sulphur
Sour ferrous iron (FeSO4), its part by weight is：0.5：1.0：2.0：2.0：5.0.
Embodiment eight
A kind of cadmium pollution soil microorganism renovation agent, it includes following component：
30 parts of Pseudomonas alcaligenes, 10 parts of bacillus subtilis, 50 parts of sulfate reducing bacteria, 20 parts of nutriment, biology
400 parts of charcoal.
The Pseudomonas alcaligenes is preserving number DSM-19550 bacterial strain；
The bacillus subtilis is preserving number DSM-8565 bacterial strain；
The sulfate reducing bacteria is Desulfotomaculum (DSM 642)；
Effective bacterium number of the Pseudomonas alcaligenes is 2.0 × 109Cfu/g, effective bacterium number of the bacillus subtilis
For 5.0 × 109Cfu/g, effective bacterium number of the sulfate reducing bacteria is 3.0 × 108cfu/g。
Nutriment dipotassium hydrogen phosphate (the K2HPO4), ammonium chloride (NH4CL), magnesium sulfate (MgSO4), sodium lactate and sulphur
Sour ferrous iron (FeSO4), its part by weight is：0.5：1.0：2.0：2.0：5.0.
Embodiment nine
A kind of cadmium pollution soil microorganism renovation agent, it includes following component：
20 parts of Pseudomonas alcaligenes, 40 parts of bacillus subtilis, 40 parts of sulfate reducing bacteria, 20 parts of nutriment, biology
300 parts of charcoal.
The Pseudomonas alcaligenes is preserving number DSM-19550 bacterial strain；
The bacillus subtilis is preserving number DSM-8565 bacterial strain；
The sulfate reducing bacteria is Desulfotomaculum (DSM 642)；
Effective bacterium number of the Pseudomonas alcaligenes is 2.0 × 109Cfu/g, effective bacterium number of the bacillus subtilis
For 5.0 × 109Cfu/g, effective bacterium number of the sulfate reducing bacteria is 3.0 × 108cfu/g。
Nutriment dipotassium hydrogen phosphate (the K2HPO4), ammonium chloride (NH4CL), magnesium sulfate (MgSO4), sodium lactate and sulphur
Sour ferrous iron (FeSO4), its part by weight is：0.5：1.0：2.0：2.0：5.0.
Embodiment ten
After taking Cd pollution paddy soils to air-dry, crush, cross 100 mesh sieves, then the microorganism passivation as described in embodiment 4-9
Agent:Soil=5:After the ratio of 1000 (weight ratios) mixes, it is fitted into diameter 40*30*40cm foam or plastic casing, Cd's can
Exchange state content is 1.21mg/kg, and native 4kg is filled per basin, adds deionized water and maintains to waterflooding state, puts Full automatic ecological culture
Cultivated in case.Impose a condition for：Day temperature is 28 DEG C, full exposure；Evening temperature is 20 DEG C, Light To Dark Ratio 12:12；Culture 30 days
It is commutative in ICP-MS methods measure soil after being well mixed afterwards with the pedotheque at four angles of soil sample collector difference and center
State Cd content.
Exchangeable species cadmium content (mg/kg) in the paddy soil of table 1
From experimental result as can be seen that exchangeable species cadmium content in soil can be greatly reduced in microorganism passivator of the present invention,
Exchangeable species Cd cut rate is between 76.6-86.3%, the soil exchangeable species Cd after passivation<0.3mg/kg, it is fixed with it is blunt
Change Cd significant effect.
Embodiment 11
After taking various concentrations Cd pollution paddy soils to air-dry, crush, cross 100 mesh sieves, measure Cd exchangeable species concentration difference
For 5.2mg/kg, 2.8mg/kg, 1.5mg/kg；Then the microorganism passivator as described in embodiment 5 and soil by weight 5:
After 1000 ratio mixes, it is fitted into diameter 40*30*40cm foam or plastic casing, initial Cd exchangeable species content difference
For 5.16,2.75 and 1.49mg/kg, native 4kg is filled per basin, deionized water is added and maintains to waterflooding state, puts Full automatic ecological training
Support in case and cultivate.Impose a condition for：Day temperature is 28 DEG C, full exposure；Evening temperature is 20 DEG C, Light To Dark Ratio 12:12；Culture 30
After being well mixed after it with the pedotheque at four angles of soil sample collector difference and center, it can be handed in ICP-MS methods measure soil
Change state Cd content.
Exchangeable species cadmium content (mg/kg) in the paddy soil of table 2
From experimental result as can be seen that microorganism passivator of the present invention is applicable Cd pollution concentrations about 1.5mg/kg rice
The passivation reparation of field soil, the soil exchangeable species Cd after passivation<3mg/kg；For the higher soil of Cd pollution concentrations (>
1.5mg/kg), although exchangeable species Cd cut rate is in 26-55.1%, exchangeable species Cd is still significantly higher than 0.3mg/
Kg further combined with other measures, it is necessary to carry out Cd pollution controls.
Claims (10)
- A kind of 1. microorganism passivator of cadmium pollution paddy soil, it is characterised in that：Including following component：Pseudomonas alcaligenes, Bacillus subtilis, sulfate reducing bacteria and carrier.
- 2. the microorganism passivator of cadmium pollution paddy soil according to claim 1, it is characterised in that：Each component weight group Into as follows：30~40 parts of Pseudomonas alcaligenes, 20~30 parts of bacillus subtilis, 20~30 parts of sulfate reducing bacteria, carrier 30 as one kind 0 ~400 parts.
- 3. the microorganism passivator of cadmium pollution paddy soil according to claim 2, it is characterised in that：Add in the preparation 10~15 parts of nutriment in a subtle way needed for biological growth.
- 4. the microorganism passivator of cadmium pollution paddy soil according to claim 2, it is characterised in that：The carrier is made a living Thing powdered carbon, the mesh of fineness 60~80.
- 5. the microorganism passivator of cadmium pollution paddy soil according to claim 2, it is characterised in that：The production alkali is false single Born of the same parents bacterium is DSM-50342 or DSM-19550, bacillus subtilis are DSM-100605 or DSM-8565 or DSM-2109, sulfuric acid Salt reducing bacteria is Desulfotomaculum DSM574 or desulfovibrio desulfurican DSM642.
- 6. the microorganism passivator of cadmium pollution paddy soil according to claim 2, it is characterised in that：Production alkali used is false Effective bacterium number of monad is 1.0-2.0 × 109Cfu/g, effective bacterium number of bacillus subtilis used for 3.0-5.0 × 109cfu/g, effective bacterium number of sulfate reducing bacteria used is 3.0-5.0 × 108cfu/g。
- 7. the microorganism passivator of cadmium pollution paddy soil according to claim 3, it is characterised in that：The nutriment Form as dipotassium hydrogen phosphate (K2HPO4), sal-ammoniac (NH4CL), magnesium sulfate (MgSO4), sodium lactate, dusty yeast and ferrous sulfate (FeSO4), its part by weight is：0.5：1.0：2.0：2.0：5.0：5.0.
- 8. the microorganism passivator of the cadmium pollution paddy soil according to any one in claim 1-7, it is characterised in that： The carrier be by straw or palm shell or wooden class biological powdered carbon prepared after high-temperature firing, dried and screened, Fineness 60-80 mesh.
- 9. the microorganism passivator of cadmium pollution paddy soil according to claim 8, it is characterised in that：The carrier is palm fibre Palmitic acid shell biological powdered carbon prepared after high-temperature firing, dried and screened, fineness 60-80 mesh.
- 10. the microbial treatment and restorative procedure of a kind of cadmium pollution soil, it is characterised in that：Butt concentration is polluted suitable for Cd The passivation reparation of below 1.5mg/kg soil；Step includes：By Pseudomonas alcaligenes, bacillus subtilis, sulfate reduction Bacterium, carrier, the nutriment mixing required for mentioned microorganism growth added if necessary；Mixed weight ratio is：It is false single to produce alkali Born of the same parents bacterium 30-40 parts, bacillus subtilis 20-30 parts, sulfate reducing bacteria 20-30 parts, nutriment 0-15 parts, carrier 30 as one kind 0- 400 parts；The biological powdered carbon, fineness 60-80 mesh；Used microorganism raw material active quantities are：Pseudomonas alcaligenes has Effect bacterium number is 1.0-2.0 × 109Cfu/g, effective bacterium number of the bacillus subtilis is 3.0-5.0 × 109Cfu/g, the sulphur Effective bacterium number of hydrochlorate reducing bacteria is 3.0-5.0 × 108cfu/g；Then it is administered to field by the dosage of 100-200kg/ mus.
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| CN108277258A (en) * | 2018-04-02 | 2018-07-13 | 中国科学院海洋研究所 | A kind of visualization qualitative checking method of sulfate reducing bacteria (SRB) |
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| CN109628099A (en) * | 2018-09-12 | 2019-04-16 | 扬州大学 | A kind of passivation stabilizer for agricultural land soil cadmium pollution reparation and preparation method thereof and application method |
| CN109628437A (en) * | 2018-12-29 | 2019-04-16 | 河北大学 | Reduce the composite passivant and the preparation method and application thereof of soil cadmium content |
| CN109705873A (en) * | 2018-12-27 | 2019-05-03 | 武汉瑞景环境修复工程有限公司 | Repair cadmium, the passivator in arsenic pollution farmland, preparation method and restorative procedure |
| CN109772877A (en) * | 2018-12-31 | 2019-05-21 | 南京格洛特环境工程股份有限公司 | Chemical pollution site remediation method |
| CN110026431A (en) * | 2019-05-20 | 2019-07-19 | 成都科泰技术有限公司 | A method of utilizing microorganism nano material in-situ cadmium pollution soil repair |
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| CN109628099A (en) * | 2018-09-12 | 2019-04-16 | 扬州大学 | A kind of passivation stabilizer for agricultural land soil cadmium pollution reparation and preparation method thereof and application method |
| CN109705873A (en) * | 2018-12-27 | 2019-05-03 | 武汉瑞景环境修复工程有限公司 | Repair cadmium, the passivator in arsenic pollution farmland, preparation method and restorative procedure |
| CN109628437A (en) * | 2018-12-29 | 2019-04-16 | 河北大学 | Reduce the composite passivant and the preparation method and application thereof of soil cadmium content |
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| CN110252803A (en) * | 2019-07-23 | 2019-09-20 | 中国科学院成都生物研究所 | A kind of cadmium pollution soil composite passivant and its application |
| CN110252803B (en) * | 2019-07-23 | 2021-04-30 | 中国科学院成都生物研究所 | Cadmium-contaminated soil composite passivator and application thereof |
| CN110776924A (en) * | 2019-11-20 | 2020-02-11 | 四川省西南大地工程物探有限公司 | Soil heavy metal passivator and preparation process thereof |
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