CN107474556A - A kind of edible packing membrane and its production and use - Google Patents
A kind of edible packing membrane and its production and use Download PDFInfo
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- CN107474556A CN107474556A CN201710798674.4A CN201710798674A CN107474556A CN 107474556 A CN107474556 A CN 107474556A CN 201710798674 A CN201710798674 A CN 201710798674A CN 107474556 A CN107474556 A CN 107474556A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D65/38—Packaging materials of special type or form
- B65D65/46—Applications of disintegrable, dissolvable or edible materials
- B65D65/463—Edible packaging materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B65—CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
- B65D—CONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
- B65D65/00—Wrappers or flexible covers; Packaging materials of special type or form
- B65D65/38—Packaging materials of special type or form
- B65D65/46—Applications of disintegrable, dissolvable or edible materials
- B65D65/466—Bio- or photodegradable packaging materials
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2201/00—Properties
- C08L2201/06—Biodegradable
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2201/00—Properties
- C08L2201/08—Stabilised against heat, light or radiation or oxydation
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2203/00—Applications
- C08L2203/16—Applications used for films
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- Health & Medical Sciences (AREA)
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Abstract
Application the invention discloses a kind of edible packing membrane and preparation method thereof and in packaging for foodstuff, its preparation method comprise the following steps：Galangal, fructus amomi and fructus alpiniae oxyphyllae are taken, respectively with alcohol solution dipping, medicinal extract is condensed into after leaching liquor centrifugation, upper macroporous resin column elution, it is galangal rhizome extract, fructus amomi extract and Fructus Alpinae Oxyphyllae extract that eluent, which is condensed into medicinal extract,；Fish scale adds EDTA solution ultrasonic decomposition decalcifications, then adds acetic acid solution and pepsin, is spray-dried supernatant after enzymolysis and fish scale collagen powder is made；The mixture of fructus amomi, fructus alpiniae oxyphyllae, galangal rhizome extract is mixed with collagen protein powder, glycerine and chitosan is added, after water-bath and ultrasound degassing, pours on instrument bezel, film is taken off after baking, obtain edible packing membrane.Edible packing membrane addition fructus amomi, fructus alpiniae oxyphyllae, the galangal rhizome extract of the present invention, strengthens the antibiotic property and inoxidizability of collagen film, reaches food fresh keeping and the effect of Shelf-life.
Description
Technical field
Application the present invention relates to a kind of edible packing membrane and preparation method thereof and in packaging for foodstuff.
Background technology
Collagen is a kind of white, opaque, unbranched fibroid protein, has natural hypotoxicity, biology
Degradability, low immunocompetence, moisture retention and trophism, it is widely used in the neck such as food industry, cosmetics, biomedical material
Domain.Collagen molecules have unique triple-helix structure, can be formed with the very big fibre bundle of tensile strength, therefore utilize
Edible film prepared by collagen not only possesses excellent film mechanical performance, and oil resistance, resistance oxygen, block water and it is fresh-keeping, protect it is fragrant
Etc. function, also it is worth with better nutritivity.
Fish scale is the material rich in native protein, predominantly collagen and keratin, account for fish scale gross weight 50~
70%, but cause substantial amounts of waste due to carrying no weight.There is experiment to show, fish scale collagen powder has anti-oxidant, drop
Blood pressure, anti-aging and other effects, and fish scale collagen powder good water solubility, easily absorb.Develop the glue of fish processing waste
Former albumen, for food, health products, cosmetics and medical material etc., the economic value added of China's processing of aquatic products is improved, simultaneously
Environmental pollution is avoided, therefore there is important economic benefit and social benefit.
With feature edible food packaging film made of natural biologic material as the research heat of food packaging applications
Point.Edible packing membrane is with edible natural macromolecular substances (such as protein, lipid, polysaccharide, cellulose and its derivates
Deng) raw material is used as, by adding the crosslinking agent, plasticizer and functional additive of edibility, by mutual between different molecular
The porous network structure for acting on and being formed, there is protection food quality and safe and healthy film.Edible collagen is packed
Film not only has some performances (such as mechanical performance, block water oxygen barrier) of other inedibility packaging films, can be in business exhibition
The advantages of showing, play a part of encasing food in product curing and preservation, but also not having with inedibility packaging film：It can drop
Solve pollution-free；Itself contains multiple nutritional components, eats beneficial to human body；Can also be by adding some sweeteners, colouring agent very
To the organoleptic properties of the improvement food such as nutrition fortifier, orectic effect is played.
The content of the invention
The present invention primary and foremost purpose be to provide a kind of preparation method of edible packing membrane, this method technique is simple, material
Material wide material sources, cost are low.
Another object of the present invention is to provide the edible packing membrane as made from the above method, the packaging film enhances anti-
The characteristic of bacterium property and inoxidizability, reach food fresh keeping and the effect of Shelf-life.
It is still another object of the present invention to provide application of the above-mentioned edible packing membrane in packaging for foodstuff.
The purpose of the present invention is achieved through the following technical solutions：
A kind of preparation method of edible packing membrane, comprises the following steps：
(1) prepared plant extracts：Galangal, fructus amomi and fructus alpiniae oxyphyllae are taken, crushing is respectively dried, then be separately added into ethanol
Solution soaks more than 3h, and then ultrasound extracts several times, each more than 2h；Then leaching liquor is centrifuged, takes supernatant, supernatant
Medicinal extract is concentrated under reduced pressure into after petroleum ether extraction degreasing and pigment；Medicinal extract upper macroporous resin column after being dissolved with ethanol solution, is first used
3BV (column volumes；Pure water elution removal of impurities more than similarly hereinafter), then eluted with more than 4BV 80% (percent by volume) ethanol solution,
Collect eluent and be concentrated under reduced pressure to give three kinds of medicinal extract, be galangal rhizome extract, fructus amomi extract and Fructus Alpinae Oxyphyllae extract respectively；
Ethanol solution preferably 80% (percent by volume) ethanol solution of step (1) described immersion；
The preferred 3000r/min centrifugations 10min of centrifugation described in step (1)；
Ethanol solution preferably 5% (percent by volume) ethanol solution of step (1) the dissolving medicinal extract；
(2) fish scale collagen powder is prepared：
1. fish scale decalcification：Take fish scale to add sodium carbonate liquor and stir 2~4h, remove foreign protein and pigment, use pure water
To neutrality, it is decalcifying agent then to add EDTA solution, simultaneously ultrasonic decomposition 2~3 times is mixed with fish scale, every time 1~2h, finally with pure
It is washed to neutrality；
Described sodium carbonate liquor, concentration preferably 0.2~0.4mol/l；
Described EDTA solution, concentration preferably 0.2~0.4mol/l；
2. prepare fish scale collagen powder：Take decalcification fish scale, add acetic acid solution and pepsin, stirring reaction 2d with
On；It is then centrifuged for collecting supernatant, is deposited in and is digested 3~5 times with the conditions of, merges supernatant；The spray-dried system of supernatant
Obtain fish scale collagen powder；
Described acetic acid solution, the too low effect for fish scale decomposition of protein of concentration is bad, and concentration more high de-agglomeration is faster, but
The too high protein inactivation that can make to decomposite, has an impact to product, so the preferred 0.4-0.6mol/l of its concentration, particularly preferably
0.5mol/l；
The preferred 1g of ratio of decalcification fish scale and acetic acid solution:10ml, the mass ratio preferably 1 of pepsin and decalcification fish scale:
100；
Centrifugation described in step (2), preferably centrifuges 15min under 4000r/min；
Spray drying described in step (2), it is as follows using SY-6000 small spraying drying instruments, technical parameter：EAT
It is set as 120~150 DEG C；Leaving air temp：70~85 DEG C；Air mass flow is set as 500~800L/H；Control pressure be 3.5~
4.0bar；Inlet amount is set as 20~40%；
(3) edible packing membrane is prepared：By fructus amomi, fructus alpiniae oxyphyllae, galangal rhizome extract made from step (1) with weight ratio
(1.0~1.5):(1.0~1.5):(1.5~3.0) mix, take 0.4~0.8g of mixture to be dissolved into 50ml distilled water；Take
3~5g of collagen protein powder made from step (2), which is dissolved into 50ml distilled water, is placed in 10~20min of water-bath in 50~60 DEG C；
The two is mixed, and sequentially adds 1.5~2.5g glycerine, 1~3g chitosans, is stirred, is placed in water-bath 10 in 50~60 DEG C
~20min, ultrasound degassing, part is pipetted according to the size of instrument bezel and poured on instrument bezel, be placed at 45~55 DEG C baking 12~
15h, film is taken off, obtain edible packing membrane；
In mixture described in step (3), fructus amomi, fructus alpiniae oxyphyllae, the weight ratio of galangal rhizome extract are 1:1:2.
The edible packing membrane as made from the above method, containing fructus amomi, fructus alpiniae oxyphyllae, galangal rhizome extract, have certain
Antibacterial and anti-oxidation characteristics, can apply in packaging for foodstuff.
The present invention is had the following advantages relative to prior art and effect：
(1) present invention makes full use of the fish scale of fishery leftover bits and pieces, meets the green theory to turn waste into wealth, improves simultaneously
The added value of fishery processing.
(2) present invention addition fructus amomi, fructus alpiniae oxyphyllae, galangal rhizome extract, strengthen the antibiotic property of collagen film and resist
Oxidisability, reach food fresh keeping and the effect of Shelf-life.
(3) raw material used in the present invention is green pollution-free, decomposable asymmetric choice net, edible material, and collagen
It is beneficial to health with the active material of fructus amomi, fructus alpiniae oxyphyllae, galangal extraction.
Embodiment
With reference to embodiment, the present invention is described in further detail, but the implementation of the present invention is not limited to this.
Embodiment
A kind of preparation method of edible packing membrane, comprises the following steps：
1. prepared plant extracts：Galangal, fructus amomi and fructus alpiniae oxyphyllae are taken, crushing is respectively dried, then be separately added into 80%
(percent by volume) more than alcohol solution dipping 3h, then ultrasonic to extract several times, each more than 2h；Then leaching liquor is centrifuged
(3000r/min centrifuges 10min), takes supernatant, supernatant is concentrated under reduced pressure into medicinal extract after petroleum ether extraction degreasing and pigment；Leaching
Cream upper macroporous resin column after being dissolved with 5% (percent by volume) ethanol solution, first with 3BV (column volumes；Pure water more than similarly hereinafter)
Elution removal of impurities, then eluted with more than 4BV 80% (percent by volume) ethanol solution, collect eluent and be concentrated under reduced pressure to give three kinds
Medicinal extract, it is galangal rhizome extract, fructus amomi extract and Fructus Alpinae Oxyphyllae extract respectively；
Fructus amomi extract, Fructus Alpinae Oxyphyllae extract, galangal rhizome extract and the mixed extract of three (mass ratio 1:1:
2) fungistatic effect assay method is as follows：
Using agar plate punch method：Four kinds of food common bacterias are respectively coated flat board, with a diameter of 6mm card punch in band
Punched on bacterium flat board, then choose the mixed extract that agar is fast, and addition 50ul concentration is 0.6g/ml in each hole, the above
Operation is carried out in superclean bench, and then in constant incubator culture 15h or so, culture terminates to observe and to measure inhibition zone straight
Footpath, it the results are shown in Table 1.
Fungistatic effect criterion：With antibacterial circle diameter>20mm is extremely quick, and antibacterial circle diameter is high between 15~20mm
Quick, antibacterial circle diameter is quick in being between 10~15, antibacterial circle diameter<10mm is muting sensitive, and 6mm is resistance without inhibition zone.
Fructus amomi extract, Fructus Alpinae Oxyphyllae extract, galangal rhizome extract and its mixed extract (mass ratio 1:1:2) it is right
DPPH and Hydroxyl radical-scavenging ability assay method are as follows：
The anti-oxidant measure of DPPH：
Prepare liquid (protein solution/(mg/ml))：0.25、0.2、0.15、0.1、0.05；
Control group：2mlDPPH+2ml ethanol；
Sample sets：2mlDPPH+2ml prepare liquids；
Blank group：2ml ethanol+2ml prepare liquids；
Zeroing group：2ml ethanol+2ml deionized waters；
Shake up and place 30min, light absorption value is surveyed at ultraviolet specrophotometer 517nm.
Clearance rate：
Y=[AControl- (ASample- ABlank)]/AControl× 100%
Hydroxyl radical-scavenging ability determines：
Sample (protein solution/(mg/ml))：0.25、0.2、0.15、0.1、0.05；
37 DEG C of water bath with thermostatic control 30min are shaken up, light absorption value is surveyed at ultraviolet specrophotometer 510nm
Clearance rate：
Y=[A1- (A2- A3)]/A1× 100%
A1During measure, reference solution is to be not added with hydrogen peroxide system；
A2、A3During measure, reference solution is deionized water.
Table 1：Antibacterial circle diameter of the plant extracts to four kinds of common bacterias in food
Note："-" is indicated without obvious inhibition zone
Table 2：Plant mixed extract is to DPPH and Hydroxyl radical-scavenging ability
As shown in Table 1, single plant extracts fungistatic effect is bad, and can not suppress all food spoilage bacterium, and
The different mixed synergies of plant extracts make fungistatic effect more obvious.
The as shown by data plant mixed extract of table 2 just has stronger oxidation resistance in low concentration, can effectively strengthen edible
Property film inoxidizability, therefore make its more anti-than simple collagen protein film by adding plant mixed extract in edible film
Bacterium property, inoxidizability are stronger, and food fresh-keeping is played an important role.
2. fish scale decalcification：The fish scale of 100g clean drying is weighed, is mixed with 0.3mol/l sodium carbonate liquors, puts agitator
Simultaneously ultrasonic decomposition 3 times are mixed after upper stirring 3h with 1L 0.2mol/l EDTA solution, each 1h, are then washed till neutrality with pure water；
3. prepare fish scale collagen solution：100g decalcification fish scales are weighed, are mixed simultaneously with 1L 0.5mol/l acetic acid solution
Add 1g pepsin (enzyme activity 1:3000) put on agitator and stir, centrifuged afterwards on the centrifuge that rotating speed is 4000r/min
15min simultaneously collects supernatant, and residual residue digests 5 times with the conditions of, merges supernatant.Above step is at ambient temperature
Carry out.
4. prepare fish scale collagen powder：Collagen liquid fast direct is switched through using SY-6000 small sprayings drying instrument
It is changed into powder granule sample.EAT is set as 125 DEG C；Leaving air temp：70~85 DEG C；Air mass flow is set as 700L/H；
Control pressure is 3.5bar；Inlet amount is set as 25%.
Gained fish scale collagen powder is shown in Table 3 to DPPH and Hydroxyl radical-scavenging ability experimental result, and experimental method is same
The upper plant mixed extract is to DPPH and the assay method of Hydroxyl radical-scavenging ability.
Table 3：Fish scale albumen powder is to DPPH and Hydroxyl radical-scavenging ability
As shown in Table 3, DPPH and Scavenging action to hydroxyl free radical can be reached respectively in the fish scale albumen powder that concentration is 0.025g/ml
To 89.1% and 76.2%, stronger oxidation resistance is shown, ensure that the antioxygenic property of edible film.
5. prepare edible film：Weigh with weight than 1:1:Fructus amomi, fructus alpiniae oxyphyllae, the galangal rhizome extract of 2 ratios mixing
0.5g is dissolved in 50ml distilled water, is taken 3g collagen protein powders to be dissolved in 50ml distilled water and is placed on water-bath in 60 DEG C
10min, the two is mixed, and sequentially add 2.5g glycerine, 2g chitosans, stirred, addition distilled water reaches cumulative volume
150ml, 60 DEG C of water-bath 10min, then place the 30min that deaerated in supersonic cleaning machine.The film liquid 150ml to have deaerated is poured into specification
In film forming device for 180mm × 90mm × 2mm, 50 DEG C of baking oven 15h are placed afterwards, film is taken off after to be dried, obtains edible packaging
Film.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (10)
1. a kind of preparation method of edible packing membrane, it is characterised in that comprise the following steps：
(1) prepared plant extracts：Galangal, fructus amomi and fructus alpiniae oxyphyllae are taken, crushing is respectively dried, then be separately added into ethanol solution
More than 3h is soaked, then ultrasound extracts several times, each more than 2h；Then leaching liquor is centrifuged, takes supernatant, supernatant is through stone
Medicinal extract is concentrated under reduced pressure into after oily ether extraction degreasing and pigment；Medicinal extract upper macroporous resin column after being dissolved with ethanol solution, first with 3BV with
On pure water elution removal of impurities, then eluted with more than 4BV 80% (percent by volume) ethanol solution, collect eluent and be concentrated under reduced pressure
Three kinds of medicinal extract are obtained, are galangal rhizome extract, fructus amomi extract and Fructus Alpinae Oxyphyllae extract respectively；
(2) fish scale collagen powder is prepared：
1. fish scale decalcification：Take fish scale to add sodium carbonate liquor and stir 2~4h, then with pure water to neutrality, then add
EDTA solution is decalcifying agent, simultaneously ultrasonic decomposition 2~3 times is mixed with fish scale, 1~2h, is finally washed till neutrality with pure water every time；
2. prepare fish scale collagen powder：Decalcification fish scale is taken, adds acetic acid solution and pepsin, more than stirring reaction 2d；So
After supernatant is collected by centrifugation, be deposited in under the conditions of digest 3~5 times, merging supernatant；The spray-dried obtained fish scale of supernatant
Collagen protein powder；
(3) edible packing membrane is prepared：By fructus amomi, fructus alpiniae oxyphyllae, galangal rhizome extract made from step (1) with weight ratio (1.0
~1.5):(1.0~1.5):(1.5~3.0) mix, take 0.4~0.8g of mixture to be dissolved into 50ml distilled water；Take step
(2) 3~5g of collagen protein powder made from, which is dissolved into 50ml distilled water, is placed in 10~20min of water-bath in 50~60 DEG C；By two
Person mixes, and sequentially adds 1.5~2.5g glycerine, 1~3g chitosans, stirs, be placed in water-bath 10 in 50~60 DEG C~
20min, ultrasound degassing, part is pipetted according to the size of instrument bezel and poured on instrument bezel, be placed at 45~55 DEG C baking 12~
15h, film is taken off, obtain edible packing membrane.
2. preparation method according to claim 1, it is characterised in that：
The ethanol solution of step (1) described immersion is 80% (percent by volume) ethanol solution；
The ethanol solution of step (1) the dissolving medicinal extract is 5% (percent by volume) ethanol solution.
3. preparation method according to claim 1, it is characterised in that：
Sodium carbonate liquor described in step (2), concentration are 0.2~0.4mol/l；
EDTA solution described in step (2), concentration are 0.2~0.4mol/l.
4. preparation method according to claim 1, it is characterised in that：In step (2), the concentration of the acetic acid solution is
0.4-0.6mol/l。
5. preparation method according to claim 1, it is characterised in that：In step (2), the matter of pepsin and decalcification fish scale
Amount is than being 1:100.
6. preparation method according to claim 1, it is characterised in that：Centrifugation described in step (2), is in 4000r/min
Lower centrifugation 15min.
7. preparation method according to claim 1, it is characterised in that：Spray drying described in step (2), using SY-
6000 small spraying drying instruments, technical parameter are as follows：EAT is set as 120~150 DEG C；Leaving air temp：70~85 DEG C；It is empty
Throughput is set as 500~800L/H；Control pressure is 3.5~4.0bar；Inlet amount is set as 20~40%.
8. preparation method according to claim 1, it is characterised in that：In mixture described in step (3), fructus amomi, benefit
Intelligence benevolence, the weight of galangal rhizome extract ratio are 1:1:2.
A kind of 9. edible packing membrane, it is characterised in that：It is to be made as the method described in claim any one of 1-8.
10. application of the edible packing membrane described in claim 9 in packaging for foodstuff.
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Cited By (7)
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| CN108929547A (en) * | 2018-06-26 | 2018-12-04 | 铜陵市明科包装技术有限公司 | The technique that grass carp scales extract prepares antibacterial film |
| CN110016232A (en) * | 2019-04-12 | 2019-07-16 | 江南大学 | A kind of fish scale collagen composite sponge preparation method |
| CN110760080A (en) * | 2019-10-10 | 2020-02-07 | 浙江海洋大学 | Method for preparing preservative film by using fish scales |
| CN110876408A (en) * | 2019-11-29 | 2020-03-13 | 广东省农业科学院蚕业与农产品加工研究所 | Special natural antibacterial liquid for cooked marinated meat and preparation method and use method thereof |
| CN112961261A (en) * | 2021-03-25 | 2021-06-15 | 中山大学 | Yangchun sand rhizome polysaccharide, preparation method thereof and anti-oxidation application thereof |
| CN113248799A (en) * | 2021-04-02 | 2021-08-13 | 浙江工商大学 | Edible polysaccharide-based food packaging film and preparation method thereof |
| CN115246946A (en) * | 2021-12-17 | 2022-10-28 | 闽南师范大学 | Preparation method of fructus amomi aqueous extract polysaccharide-based edible film |
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| CN110760080B (en) * | 2019-10-10 | 2022-06-21 | 浙江海洋大学 | Method for preparing preservative film by using fish scales |
| CN110876408A (en) * | 2019-11-29 | 2020-03-13 | 广东省农业科学院蚕业与农产品加工研究所 | Special natural antibacterial liquid for cooked marinated meat and preparation method and use method thereof |
| CN112961261A (en) * | 2021-03-25 | 2021-06-15 | 中山大学 | Yangchun sand rhizome polysaccharide, preparation method thereof and anti-oxidation application thereof |
| CN113248799A (en) * | 2021-04-02 | 2021-08-13 | 浙江工商大学 | Edible polysaccharide-based food packaging film and preparation method thereof |
| CN115246946A (en) * | 2021-12-17 | 2022-10-28 | 闽南师范大学 | Preparation method of fructus amomi aqueous extract polysaccharide-based edible film |
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