CN107468865A - The preparation technology of Pu Ling basin inflammation recovering particles - Google Patents

The preparation technology of Pu Ling basin inflammation recovering particles Download PDF

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Publication number
CN107468865A
CN107468865A CN201710796043.9A CN201710796043A CN107468865A CN 107468865 A CN107468865 A CN 107468865A CN 201710796043 A CN201710796043 A CN 201710796043A CN 107468865 A CN107468865 A CN 107468865A
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decoction
preparation technology
ethanol
alcohol extracting
steps
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林凡儒
聂昌盛
吴士平
胡百忠
毛传伟
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Xiang Yu Pharmaceutical Ltd Co
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Xiang Yu Pharmaceutical Ltd Co
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/53Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/537Salvia (sage)
    • AHUMAN NECESSITIES
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    • A61K36/23Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
    • A61K36/236Ligusticum (licorice-root)
    • AHUMAN NECESSITIES
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    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/25Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
    • A61K36/258Panax (ginseng)
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    • A61K36/28Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/288Taraxacum (dandelion)
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/46Eucommiaceae (Eucommia family), e.g. hardy rubber tree
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    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/53Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/536Prunella or Brunella (selfheal)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/185Magnoliopsida (dicotyledons)
    • A61K36/68Plantaginaceae (Plantain Family)
    • AHUMAN NECESSITIES
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/894Dioscoreaceae (Yam family)
    • A61K36/8945Dioscorea, e.g. yam, Chinese yam or water yam
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    • A61K36/18Magnoliophyta (angiosperms)
    • A61K36/88Liliopsida (monocotyledons)
    • A61K36/896Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
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    • A61K36/18Magnoliophyta (angiosperms)
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    • A61K9/16Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
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    • A61K2236/30Extraction of the material
    • A61K2236/33Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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Abstract

The invention belongs to field of medicine preparing technology, discloses the preparation technology of Pu Ling basin inflammation recovering particles, it comprises the following steps:Step 1)Weigh raw material, step 2)Ethanol percolation extracts the red sage root, step 3)Alcohol extracting Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car fare, step 4)Microwave alcohol extracting smilax and dandelion, step 5)Ultrasonic alcohol extracting Bi Collettii and selfheal, step 6)Water extraction, concentration, drying, capsule is made.Present invention process simple possible, extraction effect is good, has a extensive future.

Description

The preparation technology of Pu Ling basin inflammation recovering particles
Technical field
The invention belongs to field of medicine preparing technology, and in particular to the preparation technology of Pu Ling basin inflammation recovering particles.
Background technology
Pelvic infecton is the inflammation that pelvic inflammation refers to female pelvic cavity reproductive organs, the connective tissue of periuterine and pelvic peritoneum Disease.Etiological is postpartum or miscarriage postoperative infection, operation technique postoperative infection, menstrual hygiene be bad in uterine cavity, inflammation of adjacent organs The acute attack etc. of disease direct spreading, chronic pelvic inflammatory disease.It is divided into hydrosalpinx and tubo-ovarian cyst, salpingitis and slow Property connective tissue inflammation of pelvic cavity.The clinical manifestation of pelvic inflammation has acute and chronic two class, and the symptom of acute pelvic inflammation is lower abdomen Bitterly, heating, vaginal fluid increase, and suffer from abdominal pain as continuation, activity or sexual intercourse postemphasis, be in a bad way have shiver with cold, high fever, Headache, poor appetite, further development can cause general peritonitis, septicemia, infectious shock, severe patient to acute pelvitis of pelvic cavity Can threat to life.The symptom of chronic pelvic inflammation is lower abdomen falling inflation, and pain and lumbosacral region are ached, often after tired, sexual intercourse and Menstruation is front and rear to be aggravated, and the neurasthenia symptoms such as lassitude, the whole body uncomfortable, insomnia are may occur in which when the course of disease is long, and it is fallen ill in recent years Rate has rise trend, and this sick predisposing factors is complicated, and the state of an illness is obstinate, easy recurrent exerbation, bad when good during symptom, has a strong impact on women's Physically and mentally healthy and work.
Pu Ling basin inflammation recovering particles are that applicant independently researches and develops, and have the pharmaceutical preparation of drug batch number, have been achieved since listing Great achievement, accordingly, applicant have also applied for the patented technology on Pu Ling basin inflammation recovering particles, such as " 2006101525479, A kind of medicine for treating chronic pelvic inflammatory disease and preparation method thereof ", Pu Ling basins inflammation recovering particles on sale are protected;The patent in later stage " 2015103361326, a kind of Pu Ling basins inflammation health pharmaceutical preparation and its detection method for pelvic infecton " are scorching in known Pu Ling basins Improved on the basis of recovering particles, including 40 parts of dandelion, 35 parts of smilax, 35 parts of polygonum perfoliatum, 35 parts of the root of kudzu vine, lily 30 Part, 30 parts of rhizoma cyperi, 30 parts of the radix paeoniae rubrathe, 25 parts of field pennycress, 25 parts of golden cypress, 25 parts of dragon's blood, 25 parts of Chinese cassia tree, 20 parts of Rhizoma Sparganii, 20 parts of cattail pollen, 20 parts of Fructus Aurantii, 17 parts of myrrh, 17 parts of balloonflower root, 15 parts of the root of Dahurain angelica, 10 parts of madder, 10 parts of Poria cocos, 10 parts of the capsule of weeping forsythia, 10 parts of the tuber of stemona, gold and silver 5 parts are spent, its effect is better than known drug, but the defects of raw material type is more, and cost is high be present.
The content of the invention
The defects of in order to overcome prior art, the present invention propose the preparation technology of Pu Ling basin inflammation recovering particles, and the technique is directed to Different medicines carries out different disposal, and drug effect greatly improved, avoid the waste of raw material.
The present invention is achieved by the following technical solution:
The preparation technology of Pu Ling basin inflammation recovering particles, it comprises the following steps:Step 1)Weigh raw material, step 2)Ethanol percolation extracts The red sage root, step 3)Alcohol extracting Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car fare, step 4)Microwave alcohol extracting smilax and dandelion, step 5)Ultrasound Alcohol extracting hypoglaucous collett yam rhizome and selfheal, step 6)Water extraction, concentration, drying, capsule is made.
Further, the step 1)Raw material is weighed, is comprised the following steps:Smilax 1000g, red sage root 533.4g, summer are withered Careless 533.4g, dandelion 666.6g, plantain seed 533.4g, hypoglaucous collett yam rhizome 333.4g, bark of eucommia 333.4g, pseudo-ginseng 133.4g, Ligusticum wallichii 200g。
Further, the step 2)Ethanol percolation extracts the red sage root, comprises the following steps:The red sage root is taken, is ground into 200 purposes Powder, with 90% (v/v) of 1.5 times of weight alcohol dipping 24 hours, then add 90% (v/v) ethanol solution diacolation, filter fast 1- 3ml/min, percolate is collected, obtains decoction after percolate recovery ethanol, the dregs of a decoction are standby.
Further, the step 3)Alcohol extracting Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car fare, comprise the following steps:Take Ligusticum wallichii, Du Secondary, pseudo-ginseng and car fare, mixing, are ground into 200 mesh powders, add 70% (v/v) of 5 times of weight of crude drug alcohol dipping every time 24 hours, impregnate 3 times at room temperature, merge maceration extract, reclaim ethanol, decoction and the dregs of a decoction are standby.
Further, the step 4)Microwave alcohol extracting smilax and dandelion, comprise the following steps:Fetch earth Poria cocos and Pu public affairs English, crush, cross 200 mesh sieves, be placed in container, add the 85% of double weight(v/v)Ethanol, 300rpm stirring extraction, extraction During control the microwave power to be 500W, extraction time 120min;4 DEG C of placement 12h are subsequently placed in, filtrate is collected by filtration, filters Slag is standby.
Further, step 5)Ultrasonic alcohol extracting hypoglaucous collett yam rhizome and selfheal, comprise the following steps:Hypoglaucous collett yam rhizome and selfheal are taken, Crush, cross 200 mesh sieves, put into ultrasonic extraction tank, add the 75% of three times weight(v/v)Ethanol, control Extracting temperature be 50 DEG C, frequency 50KHz, 30min is extracted, filtrate is collected by filtration.
Further, step 6)Water extraction, concentration, drying, capsule is made, comprises the following steps:Combining step 2), step 3) And step 4)The gained dregs of a decoction, add the water of 10 times of weight, decoct 3 times, 1 hour every time, collecting decoction, filtration, then add step Rapid 2)Gained decoction, step 3)Gained decoction, step 4)Gained filtrate and step 5)Gained filtrate, mix, be concentrated under reduced pressure into The clear cream that density at 50 DEG C is 1.20g/ml, is then spray-dried, obtains dry extract, crushes, then according to 20:1 quality Than adding silica, alcohol granulation, dry, load capsule, be made 1000, produce.
The identification of medicinal material of the present invention and source:
(1)Smilax is purchased from Pingyi County Rui Kang prepared slices of Chinese crude drugs Co., Ltd, place of production Guangdong.It is limited through Shandong Xiang space health pharmacy Company is accredited as liliaceous plant smilacis glabra Smilax glabra Roxb. dry rhizome.Medicinal material removes impurity.
(2)The red sage root is purchased from Pingyi County Rui Kang prepared slices of Chinese crude drugs Co., Ltd, place of production Shandong.Have through Shandong Xiang space health pharmacy Limit company is accredited as labiate red sage root Salvia miltionrrhiza Bge. drying root and rhizome.Medicinal material removes impurity elimination Matter.
(3)Selfheal is purchased from Bozhou Shengang medicine company Co., Ltd, place of production Henan.Have through Shandong Xiang space health pharmacy Limit company is accredited as labiate selfheal Prunella vulgaris L. drying fruit ear.Medicinal material removes impurity, segment.
(4)Dandelion is purchased from Pingyi County Rui Kang prepared slices of Chinese crude drugs Co., Ltd, place of production Shandong.Through Shandong Xiang space health pharmacy Co., Ltd is accredited as feverfew dandelion Taraxacum mongolicum Hand.-Mazz. drying herb.Medicinal material removes Decontamination, segment.
(5)Plantain seed is purchased from Pingyi County Rui Kang prepared slices of Chinese crude drugs Co., Ltd, place of production Jiangxi.Through Shandong Xiang space health pharmacy Co., Ltd is accredited as plantago plant Chinese herbaceous peony Ptantago asiatica L. dry mature seed.Medicinal material removes impurity.
(6)Hypoglaucous collett yam rhizome is purchased from Pingyi County Rui Kang prepared slices of Chinese crude drugs Co., Ltd, place of production Zhejiang.Through Shandong Xiang space health pharmacy Co., Ltd is accredited as Dioscoreaceae Plant Powder back of the body Chinese yam Dioscorea hypogtauca Palibin dry rhizome.Medicinal material Thinly slice.
(7)The bark of eucommia is purchased from Pingyi County Rui Kang prepared slices of Chinese crude drugs Co., Ltd, place of production Sichuan.Have through Shandong Xiang space health pharmacy Limit company is accredited as Eucommiaceae plant bark of eucommia Eucommia ulmoides Oliv. dry bark.Medicinal material removes impurity.
(8)Pseudo-ginseng is purchased from Bozhou Shengang medicine company Co., Ltd, place of production Yunnan.It is limited through Shandong Xiang space health pharmacy Company is accredited as panax araliaceae plant Panax notoginseng (Burk.) F.H.Chen drying root and rhizome.Medicinal material removes Decontamination.
(9)Ligusticum wallichii is purchased from Pingyi County Rui Kang prepared slices of Chinese crude drugs Co., Ltd, place of production Sichuan.Have through Shandong Xiang space health pharmacy Limit company is accredited as samphire Ligusticum wallichii Ligusticum chuanxiong Hort. dry rhizome.Medicinal material removes impurity.
Relative to prior art, starting point of the invention and beneficial effect mainly include the following aspects:
Existing known Pu Ling basins inflammation recovering particles product is simply adopted to four kinds of smilax, dandelion, hypoglaucous collett yam rhizome and selfheal bulk drugs Handled with water extraction, flavonoids not soluble in water and saponins material can be caused to be lost in;Flavonoid substances in dandelion are general Water is insoluble in, possesses broad-spectrum antiseptic, clearing heat and detoxicating, mass dissipating and swelling eliminating, and polysaccharide is soluble in water possesses antibacterial, it is anti-oxidant, it is antifatigue, The functions such as immunological regulation;Smilax and Flavonoids, ursolic acid in selfheal, the triterpenes components based on oleanolic acid, Fen Bi steroid compound possess preferable anti-inflammation function, water extraction is difficult to obtain above-mentioned active ingredient;Present invention system Standby technique is directed to the deficiency of existing procucts, simple possible, different traditional Chinese medicine ingredients is handled using different technologies, improved The active ingredient of each raw material, adds drug effect, reduces wastage of material;The present invention has carried out pulverization process to raw material first, makes The powder of 200 mesh particle diameters is obtained, particle diameter is small, contributes to the rupture of cell membrane and the leaching of active principle;Smilax, dandelion carry When taking, by the way of decoction, easily cause the decomposition of phenolic acid and letones, and flavone compound can not be dissolved in Water, can not effectively it extract, the present invention obtains flavonoid glycoside compound using microwave alcohol extracting and water extraction obtains polysaccharide, avoids The loss of composition;Hypoglaucous collett yam rhizome and selfheal use ultrasonic tank alcohol extracting mode, can obtain the molten active principle of alcohol;Present invention process system Standby preparation active ingredient is high, and effect is good, and zoopery prompts its effect to be better than existing preparation, available for further clinic Research.
Embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, have below in conjunction with the application Body embodiment, the present invention is more clearly and completely described, it is clear that described embodiment is only the application one Divide embodiment, rather than whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making The every other embodiment obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Embodiment 1
The preparation technology of Pu Ling basin inflammation recovering particles, it comprises the following steps:
Step 1)Weigh raw material:Smilax 1000g, red sage root 533.4g, selfheal 533.4g, dandelion 666.6g, plantain seed 533.4g, hypoglaucous collett yam rhizome 333.4g, bark of eucommia 333.4g, pseudo-ginseng 133.4g, Ligusticum wallichii 200g.
Step 2)Ethanol percolation extracts the red sage root:The red sage root is taken, is ground into 200 mesh powders, with 90% (v/ of 1.5 times of weight V) alcohol dipping 24 hours, then add 90% (v/v) ethanol solution diacolation, filter fast 1-3ml/min, collects percolate, percolate Decoction is obtained after recovery ethanol, the dregs of a decoction are standby;
Step 3)Alcohol extracting Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car fare:Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car fare are taken, mixing, is ground into 200 Mesh powder, each plus 5 times of weight of crude drug 70% (v/v) alcohol dipping 24 hours, impregnates 3 times at room temperature, merges leaching Stain liquid, ethanol is reclaimed, decoction and the dregs of a decoction are standby;
Step 4)Microwave alcohol extracting smilax and dandelion:Fetch earth Poria cocos and dandelion, crush, cross 200 mesh sieves, be placed in container, Add the 85% of double weight(v/v)Ethanol, 300rpm stirring extractions, it is 500W that microwave power control in extraction process, is extracted Time is 120min;4 DEG C of placement 12h are subsequently placed in, filtrate are collected by filtration, filter residue is standby;
Step 5)Ultrasonic alcohol extracting hypoglaucous collett yam rhizome and selfheal:Hypoglaucous collett yam rhizome and selfheal are taken, is crushed, crosses 200 mesh sieves, puts into ultrasonic extraction Tank, add the 75% of three times weight(v/v)Ethanol, control Extracting temperature be 50 DEG C, frequency 50KHz, extract 30min, filtering receive Collect filtrate;
Step 6)Water extraction, concentration, drying, capsule is made:Combining step 2), step 3)And step 4)The gained dregs of a decoction, add 10 times The water of weight, decoct 3 times, 1 hour every time, collecting decoction, filtration, then add step 2)Gained decoction, step 3)Gained medicine Liquid, step 4)Gained filtrate and step 5)Gained filtrate, mix, it is 1.20g/ml's to be concentrated under reduced pressure into the density at 50 DEG C Clear cream, then it is spray-dried, obtains dry extract, crushes, then according to 20:1 mass ratio addition silica(20 parts of dry extracts add Add 1 part of silica), alcohol granulation, dry, load capsule, be made 1000, produce.Every loading is 0.45g.
Comparative example 1
Pu Ling basin inflammation recovering particles, it includes the bulk drug of following weight:
Smilax 1000g, red sage root 533.4g, selfheal 533.4g, dandelion 666.6g, plantain seed 533.4g, hypoglaucous collett yam rhizome 333.4g, bark of eucommia 333.4g, pseudo-ginseng 133.4g, Ligusticum wallichii 200g.
It is prepared according to following technique:
Remove above-mentioned raw materials;
The red sage root is ground into 80 mesh coarse powder, with the alcohol dipping 24 hours of 1.5 times of weight proportions 90% (v/v), then adds 90% (v/v) Ethanol solution diacolation, filter fast 1-3ml/min, collects 5 times amounts of the percolate for crude drug weight, the first liquid of filtering of 1 times of amount of crude drug weight Standby, remaining percolate recovery ethanol, decoction and the dregs of a decoction are standby;
Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car fare are taken, adds 70% (v/v) alcohol dipping of 5 times of weight of crude drug every time 24 hours, in room The lower dipping of temperature 3 times, merges maceration extract, reclaims ethanol, decoction and the dregs of a decoction are standby;
Fetch earth the dregs of a decoction of Poria cocos, selfheal, dandelion, hypoglaucous collett yam rhizome and the above-mentioned red sage root dregs of a decoction, Ligusticum wallichii etc., adds 10 times of weight Water, decoct 3 times, 1 hour every time, collecting decoction, filter, concentration, danshen solution and Ligusticum wallichii for adding after above-mentioned recovery ethanol etc. Decoction, continue to be concentrated under reduced pressure into the clear cream that density at 50 DEG C is 1.20, be dried under reduced pressure, obtain dry extract,
Auxiliary material is added by the 4: 1 of crude drug weight again, described auxiliary material is Icing Sugar: dextrin=3: 1 weight proportion, is then crushed, mistake 80 mesh sieves, mix, obtain dry extract;Particle is made in liquid and 80% (v/v) ethanol of just being filtered with the red sage root, through drying, whole grain, loads Capsule, 1000 are made, is produced.Every loading is 0.45g.
The Experiment of Zoology of embodiment 2
Establish pelvic infecton mouse model:From healthy Kunming Strains of Mouse, mouse pelvic infecton model is built.According to Mice Body Mouse, general anesthesia, is placed on fixed station after several minutes, subtracts belly wool by 1% 4% chloraldurate of intraperitoneal injection of weight, 75% ethanol disinfection skin, the scissors tweezers of sterilizing are taken, cut off mouse lower abdomen skin and muscle layer, about 1cm otch, cruelly Expose uterus, take aseptic syringe needle to stab uterus side cornua uteri centre position, about 5-6 pins, after layering close abdomen, sterilize belly Art area.
Experiment flow:From the mouse of above modeling, blank control group (without any processing), vacation are randomly divided into after 7d Operation group (only doing abdominal, do not handle uterus), model group, embodiment 1 group (20mg/d), reference examples 1 group (20mg/d), Every group 10;In addition to the pure water that blank control, sham-operation group and model group such as give at the capacity, remaining give accordingly by Test agent, continuous gavage 21d.It is discontinued next day, mouse is weighed, takes serum and put to death, take uterus to weigh after dissection, and uterus is used 4% neutral formalin fixer is fixed, FFPE, conventional section;HE is dyed, light Microscopic observation uterus morphological changes of various tissue components. The testing result of mice serum index cell factor is shown in Table 1.
Table 1
Group Interleukin-22(pg/ml) Interleukin 6(pg/ml) Tumor necrosis factor α(pg/ml)
Blank control group 506.3 42.1 215.2
Sham-operation group 491.7 41.3 218.5
Model group 470.5 44.9 242.1
1 group of embodiment 609.8 35.8 182.9
1 group of reference examples 547.1 38.4 207.6
Conclusion:As shown in Table 1, model group reduces compared with the content of the interleukin-22 of blank control group, and interleukin 6 and neoplasm necrosis Factor-alpha greatly improves, and illustrates modeling success.The interleukin-22 content of embodiment 1 is higher than 1 group of reference examples, and interleukin 6 and tumour Necrosin alpha content is less than 1 group of reference examples, prompts to be better than 1 group of reference examples to the improvement result of pelvic inflammation.
Uterus pathology observation index and ranking criterion are:(1) Asherman's syndrom locking or expansion:"-" remembers 0 point without lesion; The lesions of "+" < 1/3 remember 1 point;" ++ " 1/3-2/3 lesion remembers 2 points;" +++ " more than 2/3 lesion remembers 3 points.(2) cavity wall structure disease Become:Each Rotating fields of "-" normally remember 0 point;"+" mucosa lamina propria gland structure, which disappears, remembers 1 point;" ++ " muscle layer is demarcated with mucous layer Unclear 2 points of note;Unclear 3 points of the note of " +++ " hierarchy.(3) epithelial cell degeneration necrosis:"-" simple columnar epithelium remembers 0 point; "+" epithelial cell is flat or the < 1/3 that comes off remembers 1 point;" ++ " epithelial cell is flat or 1/3-2/3 lesion that comes off remembers 2 points;“+++” Holostrome epithelial cell degeneration necrosis remembers 3 points.(4) chronic cell infiltration:"-" remembers 0 point without chronic cell infiltration;"+" decimal Be dispersed in or stove and only in mucosa lamina propria remember 1 point;" ++ " be dispersed in or stove but go deep into muscle layer remember 2 points;" +++ " majority is dispersed in Or stratiform infiltrates and involves holostrome and remembers 3 points.(5) inner membrance congestion and edema:"-" remembers 0 point without inner membrance congestion and edema;"+" lamina propria is light Micro- congestion and edema remembers 1 point;" ++ " obvious congestion and edema remembers 2 points;" +++ " holostrome congestion and edema remembers 3 points.Uterus pathological change scoring It the results are shown in Table 2:
Table 2
Group Asherman's syndrom locking or expansion Cavity wall structural lesions Epithelial cell degeneration necrosis Chronic cell infiltration Inner membrance congestion and edema Gross score
Blank control group 0 0 0 0 0 0
Sham-operation group 1 1 0 2 1 5
Model group 6 7 4 4 7 28
1 group of embodiment 1 1 1 1 3 7
1 group of reference examples 1 2 2 2 4 11
Conclusion:As shown in Table 2, the product of 1 group of embodiment 1 and reference examples can be obviously improved the lesion degree in uterus, but real The product effect for applying example 1 is best.
Embodiment 3
Target level of product quality test of the present invention:In order to verify and improve the rational research of laboratory process, we connect product Three batches of continuous production, and pilot product is detected by the regulation of quality standard, it the results are shown in Table 3.
Table 3
Batch Astilbin content(Mg/ grains) Tanshinone IIAContent(Mg/ grains) Bacterium(cfu/g) Mould(cfu/g) EHEC
1 3.38 0.57 <10 <10 Do not detect
2 3.34 0.54 <10 <10 Do not detect
3 3.35 0.56 <10 <10 Do not detect
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specific example " or The description of " some examples " etc. means that combining specific features, structure, material or feature that the embodiment or example describe includes In at least one embodiment or example of the present invention.In this manual, to the schematic representation of above-mentioned term necessarily pin To be identical embodiment or example.Although embodiments of the invention have been shown and described above, it is to be understood that Above-described embodiment is exemplary, it is impossible to is interpreted as limitation of the present invention, one of ordinary skill in the art is the present invention's In the range of above-described embodiment can be changed, change, replace and modification.

Claims (7)

1. the preparation technology of Pu Ling basin inflammation recovering particles, it comprises the following steps:Step 1)Weigh raw material, step 2)Ethanol percolation carries Take the red sage root, step 3)Alcohol extracting Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car fare, step 4)Microwave alcohol extracting smilax and dandelion, step 5)It is super Sound alcohol extracting hypoglaucous collett yam rhizome and selfheal, step 6)Water extraction, concentration, drying, capsule is made.
2. preparation technology according to claim 1, it is characterised in that the step 1)Raw material is weighed, is comprised the following steps: Smilax 1000g, red sage root 533.4g, selfheal 533.4g, dandelion 666.6g, plantain seed 533.4g, hypoglaucous collett yam rhizome 333.4g, Du Secondary 333.4g, pseudo-ginseng 133.4g, Ligusticum wallichii 200g.
3. preparation technology according to claim 1, it is characterised in that the step 2)Ethanol percolation extracts the red sage root, including Following steps:The red sage root is taken, is ground into 200 mesh powders, with 90% (v/v) of 1.5 times of weight alcohol dipping 24 hours, then is added 90% (v/v) ethanol solution diacolation, filter fast 1-3ml/min, collects percolate, decoction, the dregs of a decoction are obtained after percolate recovery ethanol It is standby.
4. preparation technology according to claim 1, it is characterised in that the step 3)Alcohol extracting Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car Qian Zi, comprise the following steps:Ligusticum wallichii, the bark of eucommia, pseudo-ginseng and car fare are taken, mixing, 200 mesh powders is ground into, adds crude drug 5 every time 70% (v/v) of times weight alcohol dipping 24 hours, impregnate 3 times at room temperature, merge maceration extract, reclaim ethanol, decoction and The dregs of a decoction are standby.
5. preparation technology according to claim 1, it is characterised in that the step 4)Microwave alcohol extracting smilax and Pu are public English, comprise the following steps:Fetch earth Poria cocos and dandelion, crush, cross 200 mesh sieves, be placed in container, add the 85% of double weight (v/v)Ethanol, 300rpm stirring extractions, it is 500W, extraction time 120min that microwave power is controlled in extraction process;Then 4 DEG C of placement 12h are placed in, filtrate are collected by filtration, filter residue is standby.
6. preparation technology according to claim 1, it is characterised in that step 5)Ultrasonic alcohol extracting hypoglaucous collett yam rhizome and selfheal, bag Include following steps:Hypoglaucous collett yam rhizome and selfheal are taken, is crushed, crosses 200 mesh sieves, puts into ultrasonic extraction tank, adds the 75% of three times weight (v/v)Ethanol, control Extracting temperature be 50 DEG C, frequency 50KHz, extract 30min, filtrate is collected by filtration.
7. the preparation technology according to claim 1-6 is allowed to one, it is characterised in that step 6)Water extraction, concentration, drying, system Into capsule, comprise the following steps:Combining step 2), step 3)And step 4)The gained dregs of a decoction, add the water of 10 times of weight, decoct 3 It is secondary, 1 hour every time, collecting decoction, filtration, then add step 2)Gained decoction, step 3)Gained decoction, step 4)Gained is filtered Liquid and step 5)Gained filtrate, mix, be concentrated under reduced pressure into the clear cream that the density at 50 DEG C is 1.20g/ml, then spraying is dry It is dry, dry extract is obtained, is crushed, then according to 20:1 mass ratio addition silica, alcohol granulation, dries, loads capsule, i.e., .
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Application publication date: 20171215