CN107460125A - Suspending cell culture bottle - Google Patents
Suspending cell culture bottle Download PDFInfo
- Publication number
- CN107460125A CN107460125A CN201710944025.0A CN201710944025A CN107460125A CN 107460125 A CN107460125 A CN 107460125A CN 201710944025 A CN201710944025 A CN 201710944025A CN 107460125 A CN107460125 A CN 107460125A
- Authority
- CN
- China
- Prior art keywords
- bottle
- fixed plate
- bottleneck
- drive mechanism
- portable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 17
- 238000007789 sealing Methods 0.000 claims abstract description 16
- 244000309464 bull Species 0.000 claims description 11
- 241000826860 Trapezium Species 0.000 claims description 2
- 230000006835 compression Effects 0.000 claims description 2
- 238000007906 compression Methods 0.000 claims description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 19
- 230000001413 cellular effect Effects 0.000 abstract description 3
- 238000002955 isolation Methods 0.000 abstract description 3
- 210000004027 cell Anatomy 0.000 description 19
- 239000000725 suspension Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 3
- 239000012930 cell culture fluid Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000008450 motivation Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000004804 winding Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
Landscapes
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of suspending cell culture bottle, including：Bottle, bottleneck, bottle cap, some air-vents being arranged on bottle cap, fixed plate, fixed plate drive mechanism, portable plate and portable plate drive mechanism.During bottle cap screwing, two portable plates move up to contract, therefore when cell gradually suspends from bottle both sides, realize the culture that suspends.Bottle cap is opened, portable plate drive mechanism drives two portable plate opposing fixed plates, and both sides are deployed to the left and right, and two rubber strips III are in sealing contact with bottle left and right sides respectively.The cellular portions that now fixed plate and portable plate are suspended upper end in bottle by rubber strip I, rubber strip II and rubber strip III realize sealing isolation, the nutrient solution of bottle the latter half adds new nutrient solution from bottleneck again afterwards by being poured out after support from bottleneck.The phenomenon of cell will not be polluted, saves nutrient solution, improves the efficiency for changing nutrient solution.
Description
Technical field
The present invention relates to cell culture experiments fixture fields, and in particular to a kind of suspending cell culture bottle.
Background technology
Cell culture changes liquid for normal 2-3 days 1 time, and wherein suspension cell, which changes liquid process, is：From cell culture incubator by cell culture
Bottle takes out, and enters aseptic operating platform, and suction pipe draws cell and liquid in bottle, is transferred to centrifuge tube, centrifuges, draws, abandons supernatant, adds
New culture medium,（Such as passage, then after this step -- cell, adjustment density is resuspended, then is separately added into ... ..）Add and contain
There is the blake bottle of new serum and culture medium, insert incubator and continue to cultivate.Changed if for suspension stem cell sphere culture using half amount
Liquid method, also needing first to hold up blake bottle standing half an hour, make cell precipitation, suction pipe draws culture supernatants and abandons it, then after the capable above
Continuous process.In the process, the processes such as suction pipe is drawn, centrifuge tube centrifuges easily consume cell, make its regrowth slack-off；In addition centrifuge
Cell need to take out blake bottle from aseptic operating platform, return aseptic operating platform after centrifugation again, and this process has contamination of cells risk, once it is dirty
Dye can make it dead；And then repeat culture and cause the increase of reagent consumables cost, and virtually influence experiment progress, extension experiment are total
Time.
The content of the invention
The present invention is in order to overcome the shortcomings of above technology, there is provided one kind prevents cell depletion pollution, saves the operating time
And save the suspending cell culture bottle of nutrient solution.
Technical scheme is used by the present invention overcomes its technical problem：
A kind of suspending cell culture bottle, including：The closed bottle of surrounding, tilt upward and be arranged at bottle head end bottleneck, pass through spiral shell
Line coordinates the bottle cap being combined on bottleneck, some air-vents being arranged on bottle cap, is horizontally placed on bottle along bottle length direction
Internal fixed plate, it is arranged at the fixed plate drive mechanism, horizontal along width respectively for being used to drive fixed plate to move up and down on bottle
Degree direction is slidably mounted on the portable plate and portable plate drive mechanism of left and right ends below fixed plate drive mechanism, the fixation
Plate rear end is provided with rubber strip I along bottle width, and the rubber strip I contacts with bottle back-end sealing, before the fixed plate
End is provided with rubber strip II along bottle width, and the rubber strip II and bottle front end and bottle mouth position are in sealing contact, the work
Dynamic plate outboard end is provided with rubber strip III along bottle length direction, and when bottle cap is combined on bottleneck, bottle cap is driven by portable plate
Motivation structure drives two portable plate opposing fixed plates, and to contract, when bottle cap is removed from bottleneck, portable plate drive mechanism is driven
Moving two portable plate opposing fixed plates, both sides are deployed to the left and right, and two rubber strips III connect with the sealing of bottle left and right sides respectively
Touch.Guide pillar that above-mentioned fixed plate drive mechanism is vertically arranged on bottle, it is vertically arranged to fixed plate lower end and slides be plugged in
Sliding sleeve on guide pillar, the spring I being set on guide pillar and it is vertical be rotatablely installed it is same in the bull stick on bottle, the bull stick lower end
Axle is provided with screw rod, and the screw rod is combined in fixed plate, and the bull stick upper end is provided with handle, the upper end of spring I and cunning
Set is connected, and its lower end is connected with bottle.
It is in criss-cross support, with axially parallel with bottleneck that above-mentioned portable plate drive mechanism, which is included being arranged in bottleneck,
Direction slide be plugged in support push rod, be arranged at the pin shaft II of push rod tail end, be arranged at fixed plate lower end bearing, draw
Spring and the left and right sides are respectively the cone block on inclined-plane, and the fixed plate is interior and is arranged above and cone block matching positioned at cone block
Groove, the both ends of extension spring are connected with two portable plates respectively, and the inclined-plane of the cone block of the portable plate medial extremity and homonymy is slided
Dynamic contact, connecting rod middle part are installed on bearing by the way that bearing pin I is be hinged, and its tail end is connected with boring block, and its head end is along length
Direction is provided with elongated hole, and the pin shaft II is slidably mounted in elongated hole, and boss is provided with the push rod, and spring II, which is set in, to be pushed away
On bar, its head end is connected with boss, and its tail end is connected with support, when bottle cap is screwed in bottleneck, the inside Slideslip of push rod and pressure
Contracting spring II.
It is bulb to reduce the head end of the above-mentioned push rod of frictional force.
In order that suspension cell smoothly floats, it is respectively inclined-plane at left and right sides of above-mentioned fixed plate, fixed plate is formed under width
Narrow trapezium structure.
The beneficial effects of the invention are as follows：In use, opening bottle cap, cell culture fluid is added in bottle from bottle mouth position, it
After screw bottle cap, by air-vent air inlet, during bottle cap screwing, two portable plates are to contract, therefore cell gradually suspends
When, moved up from bottle both sides, realize the culture that suspends.When needing to change nutrient solution, bottle cap, portable plate drive mechanism driving are opened
Two portable plate opposing fixed plates to the left and right deploy by both sides, and two rubber strips III are in sealing contact with bottle left and right sides respectively.
The cellular portions that now fixed plate and portable plate are suspended upper end in bottle by rubber strip I, rubber strip II and rubber strip III
Sealing isolation is realized, the nutrient solution of bottle the latter half adds newly from bottleneck again afterwards by being poured out after support from bottleneck
Nutrient solution.After adding new nutrient solution, bottle cap is screwed on bottleneck again, portable plate drive mechanism two portable plates again
To contract, contact of the suspension cell with nutrient solution is now realized again, and realization is cultivated again.Showing for cell will not be polluted
As saving nutrient solution, improving the efficiency for changing nutrient solution.
Brief description of the drawings
Fig. 1 is the structural representation of the present invention；
Fig. 2 is the structural representation of the support portions of the present invention；
Fig. 3 is the side structure schematic view at the slide plate position of the present invention；
Fig. 4 is the mplifying structure schematic diagram at the slide bar position of the present invention；
In figure, the sliding sleeve of 1. bottle, 2. fixed plate, 3. portable plate, 4. rubber strip, I 5. rubber strip, II 6. bottleneck, 7. guide pillar 8.
9. the bearing 18. of 16. support of spring I 10. connecting rod, 11. bull stick, 12. screw rod, 13. handle, 14. bottle cap, 15. air-vent 17.
The rubber strip III 24. of 22. inclined-plane of bearing pin I 19. push rod, 20. boss, 21. groove 23. bores the bulb 27. of 25. extension spring of block 26.
The elongated hole of II 28. pin shaft II of spring 29..
Embodiment
1 to accompanying drawing 4, the present invention will be further described below in conjunction with the accompanying drawings.
A kind of suspending cell culture bottle, including：The closed bottle 1 of surrounding, tilt upward be arranged at the head end bottleneck 6 of bottle 1,
The bottle cap 14 being combined on bottleneck 6, some air-vents 15 being arranged on bottle cap 14 are coordinated, along the length side of bottle 1 by screw thread
Driven to the fixed plate 2 being horizontally placed in bottle 1, the fixed plate being arranged on bottle 1 for driving fixed plate 2 to move up and down
Mechanism, the respectively horizontal portable plate 3 for being slidably mounted on fixed plate drive mechanism lower section left and right ends in the width direction and activity
Plate drive mechanism, the rear end of fixed plate 2 are provided with rubber strip I 4, rubber strip I 4 and the back-end sealing of bottle 1 along the width of bottle 1
Contact, the front end of fixed plate 2 are provided with rubber strip II 5, rubber strip II 5 and the front end of bottle 1 and bottleneck 6 along the width of bottle 1
Locate in sealing contact, the outboard end of portable plate 3 is provided with rubber strip III 23 along the length direction of bottle 1, when bottle cap 14 is combined on bottleneck 6
When upper, bottle cap 14 drives two opposing fixed plates 2 of portable plate 3 to contract by portable plate drive mechanism, when bottle cap 14 is from bottle
When being removed on mouth 6, portable plate drive mechanism drives two opposing fixed plates 2 of portable plate 3, and both sides are deployed to the left and right, two rubber strips
III 23 is in sealing contact with the left and right sides of bottle 1 respectively.In use, opening bottle cap 14, cell culture fluid is added at bottleneck 6
Enter in bottle 1, screw bottle cap 14 afterwards, by the air inlet of air-vent 15, when bottle cap 14 screws, two portable plates 3 are inwardly received
Contracting, therefore when cell gradually suspends, moved up from the both sides of bottle 1, realize the culture that suspends.When needing to change nutrient solution, bottle is opened
Lid 14, portable plate drive mechanism drives two opposing fixed plates 2 of portable plate 3, and both sides are deployed to the left and right, two III 23 points of rubber strips
It is not in sealing contact with the left and right sides of bottle 1.Now fixed plate 2 and portable plate 3 by rubber strip I 4, rubber strip II 5 and
The cellular portions that rubber strip III 23 suspends upper end in bottle 1 realize sealing isolation, and the nutrient solution of the latter half of bottle 1 passes through
Poured out after support 16 from bottleneck 6, add new nutrient solution from bottleneck 6 again afterwards.After adding new nutrient solution, again will
Bottle cap 14 is screwed on bottleneck 6, and two portable plates 3 to contract, now realize suspension cell to portable plate drive mechanism again again
Contact with nutrient solution, realization are cultivated again.The phenomenon of cell will not be polluted, saves nutrient solution, improves replacing training
The efficiency of nutrient solution.
By fixed plate drive mechanism can with the height of adjustment fixing plate 2, so as to ensure under different liquid measure environment,
Smoothly suspension cell is split.
Fixed plate drive mechanism can be following structure, including be vertically arranged on bottle 1 guide pillar 7, be vertically arranged to
The lower end of fixed plate 2 and slip are plugged in the sliding sleeve 8 on guide pillar 7, the spring I 9 being set on guide pillar 7 and vertical rotational installation
In the bull stick 11 on bottle 1, the lower end of bull stick 11 is coaxially arranged with screw rod 12, and screw rod 12 is combined in fixed plate 2, the upper end of bull stick 11
Handle 13 is provided with, the upper end of spring I 9 is connected with sliding sleeve 8, and its lower end is connected with bottle 1.Fixed when needing to be adjusted according to liquid measure
During 2 height and position of plate, rotating handles 13 makes it drive bull stick 11 to rotate, and in the presence of the rotation of screw rod 12, it drives fixed plate 2
Move up and down, fixed plate 2 move up and down during by sliding sleeve 8 along 7 slide-and-guides are caused, improve flat in its motion process
Stability, while spring I 9 plays elastic buffer effect.
Portable plate drive mechanism can be following structure, it include being arranged in bottleneck 6 in criss-cross support 16, with
Slide the push rod 19 being plugged in support 16, be arranged at the pin shaft II of the tail end of push rod 19 in the direction parallel with the axial direction of bottleneck 6
28th, bearing 17, extension spring 25 and the left and right sides for being arranged at the lower end of fixed plate 2 are respectively the cone block 24 on inclined-plane, in fixed plate 2 and
Positioned at the groove 21 for being arranged above matching with cone block 24 of cone block 24, the both ends of extension spring 25 respectively with two phases of portable plate 3
The inclined-plane sliding contact of the cone block 24 of connection, the medial extremity of portable plate 3 and homonymy, the middle part of connecting rod 10 are be hinged by bearing pin I 18
It is installed on bearing 17, its tail end is connected with cone block 24, and its head end is provided with elongated hole 29 along its length, and pin shaft II 28 is slided
It is installed in elongated hole 29, boss 20 is provided with push rod 19, spring II 27 is set on push rod 19, its head end and the phase of boss 20
Even, its tail end is connected with support 16.When bottle cap 14 is screwed in bottleneck 6, push rod 19 is promoted by bottle cap 14, makes its inside Slideslip
And compression spring II 27.Push rod 19 moves inward it makes connecting rod 10 be rotated around bearing pin I 18 by pin shaft II 28, bearing pin I
18 driving cone blocks 24 move up, and insert it into groove 21, during cone block 24 moves up, in the presence of extension spring 25, and portable plate 3
Move inward, so as to ensure that suspension cell training is floated.After bottle cap 14 is outwarded winding, spring II 27 promote push rod 19 to
Outside is moved, and push rod 19 pulls connecting rod 10 to rotate backward by pin shaft II 28, is moved down so as to bore block 24, and two portable plates 3 are along cone
The inclined-plane of block 24 be oriented under horizontally outward side movement until rubber strip III 23 and the left and right sides wall of bottle 1 it is in sealing contact, make bottle 1
Epicoele seals.
Preferably, the head end of push rod 19 is bulb 26, and bulb 26 and the inner face contact area of bottle cap 14 are small, reduce friction
Resistance, effectively increase the life-span of bottle cap.The left and right sides of fixed plate 2 is respectively inclined-plane 22, and fixed plate 2 forms ladder wide at the top and narrow at the bottom
Shape structure.When suspension cell culture, the cell of suspension can prevent suspension cell solid under the guiding on inclined-plane 22 to floating
The lower end of fixed board 2 forms aggregation.
Claims (5)
- A kind of 1. suspending cell culture bottle, it is characterised in that including：The closed bottle of surrounding（1）, tilt upward and be arranged at bottle （1）Head end bottleneck（6）, by screw thread coordinate be combined on bottleneck（6）On bottle cap（14）, be arranged at bottle cap（14）Parching on if Stomata（15）, along bottle（1）Length direction is horizontally placed on bottle（1）Interior fixed plate（2）, be arranged at bottle（1）On be used for Drive fixed plate（2）The fixed plate drive mechanism of up and down motion, the horizontal fixed plate that is slidably mounted in the width direction drives respectively The portable plate of left and right ends below mechanism（3）And portable plate drive mechanism, the fixed plate（2）Rear end is along bottle（1）Width Direction is provided with rubber strip I（4）, the rubber strip I（4）With bottle（1）Back-end sealing contacts, the fixed plate（2）Front end edge Bottle（1）Width is provided with rubber strip II（5）, the rubber strip II（5）With bottle（1）Front end and bottleneck（6）Place's sealing Contact, the portable plate（3）Outboard end is along bottle（1）Length direction is provided with rubber strip III（23）, work as bottle cap（14）It is combined on Bottleneck（6）When upper, bottle cap（14）Two portable plates are driven by portable plate drive mechanism（3）Opposing fixed plate（2）To contract, Work as bottle cap（14）From bottleneck（6）On when removing, portable plate drive mechanism drives two portable plates（3）Opposing fixed plate（2）To the left Right both sides expansion, two rubber strips III（23）Respectively with bottle（1）Left and right sides are in sealing contact.
- 2. suspending cell culture bottle according to claim 1, it is characterised in that：The fixed plate drive mechanism is vertically-mounted In bottle（1）On guide pillar（7）, be vertically arranged to fixed plate（2）Lower end and slip be plugged in guide pillar（7）On sliding sleeve（8）, set Loaded on guide pillar（7）On spring I（9）And it is rotatablely installed vertically in bottle（1）On bull stick（11）, the bull stick（11）Lower end It is coaxially arranged with screw rod（12）, the screw rod（12）It is combined on fixed plate（2）In, the bull stick（11）Upper end is provided with handle （13）, the spring I（9）Upper end and sliding sleeve（8）It is connected, its lower end and bottle（1）It is connected.
- 3. suspending cell culture bottle according to claim 1, it is characterised in that：The portable plate drive mechanism includes setting In bottleneck（6）In be in criss-cross support（16）, with bottleneck（6）Axially parallel direction is slided and is plugged in support（16） In push rod（19）, be arranged at push rod（19）The pin shaft II of tail end（28）, be arranged at fixed plate（2）The bearing of lower end（17）, draw Spring（25）And the left and right sides is respectively the cone block on inclined-plane（24）, the fixed plate（2）It is interior and positioned at cone block（24）Surface It is provided with and cone block（24）The groove of matching（21）, extension spring（25）Both ends respectively with two portable plates（3）It is connected, the work Dynamic plate（3）The cone block of medial extremity and homonymy（24）Inclined-plane sliding contact, connecting rod（10）Middle part passes through bearing pin I（18）It is be hinged It is installed on bearing（17）On, its tail end and cone block（24）It is connected, its head end is provided with elongated hole along its length（29）, the pin Axle II（28）It is slidably mounted on elongated hole（29）In, the push rod（19）On be provided with boss（20）, spring II（27）It is set in and pushes away Bar（19）On, its head end and boss（20）It is connected, its tail end and support（16）It is connected, works as bottle cap（14）Screw in bottleneck（6）When middle, Push rod（19）Inside Slideslip and compression spring II（27）.
- 4. suspending cell culture bottle according to claim 3, it is characterised in that：The push rod（19）Head end be bulb （26）.
- 5. according to the suspending cell culture bottle described in claim 1 or 2 or 3, it is characterised in that：The fixed plate（2）Left and right two Side is respectively inclined-plane（22）, fixed plate（2）Form trapezium structure wide at the top and narrow at the bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710944025.0A CN107460125A (en) | 2017-10-12 | 2017-10-12 | Suspending cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710944025.0A CN107460125A (en) | 2017-10-12 | 2017-10-12 | Suspending cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107460125A true CN107460125A (en) | 2017-12-12 |
Family
ID=60554138
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710944025.0A Pending CN107460125A (en) | 2017-10-12 | 2017-10-12 | Suspending cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107460125A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108715809A (en) * | 2018-06-08 | 2018-10-30 | 天晴干细胞股份有限公司 | A kind of space station Tissue Culture Flask and auxiliary device |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
| US20210230528A1 (en) * | 2018-07-13 | 2021-07-29 | Corning Incorporated | Cell culture vessels with stabilizer devices |
| US11661574B2 (en) | 2018-07-13 | 2023-05-30 | Corning Incorporated | Fluidic devices including microplates with interconnected wells |
| US11857970B2 (en) | 2017-07-14 | 2024-01-02 | Corning Incorporated | Cell culture vessel |
| US11912968B2 (en) | 2018-07-13 | 2024-02-27 | Corning Incorporated | Microcavity dishes with sidewall including liquid medium delivery surface |
| US11970682B2 (en) | 2017-07-14 | 2024-04-30 | Corning Incorporated | 3D cell culture vessels for manual or automatic media exchange |
| US11976263B2 (en) | 2014-10-29 | 2024-05-07 | Corning Incorporated | Cell culture insert |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204918617U (en) * | 2015-06-05 | 2015-12-30 | 北京和实康明医药科技有限公司 | Cell culture bottle |
-
2017
- 2017-10-12 CN CN201710944025.0A patent/CN107460125A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN204918617U (en) * | 2015-06-05 | 2015-12-30 | 北京和实康明医药科技有限公司 | Cell culture bottle |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11976263B2 (en) | 2014-10-29 | 2024-05-07 | Corning Incorporated | Cell culture insert |
| US11857970B2 (en) | 2017-07-14 | 2024-01-02 | Corning Incorporated | Cell culture vessel |
| US11970682B2 (en) | 2017-07-14 | 2024-04-30 | Corning Incorporated | 3D cell culture vessels for manual or automatic media exchange |
| CN108715809A (en) * | 2018-06-08 | 2018-10-30 | 天晴干细胞股份有限公司 | A kind of space station Tissue Culture Flask and auxiliary device |
| CN108715809B (en) * | 2018-06-08 | 2022-03-11 | 天晴干细胞股份有限公司 | Space station cell culture bottle and auxiliary device |
| US20210230528A1 (en) * | 2018-07-13 | 2021-07-29 | Corning Incorporated | Cell culture vessels with stabilizer devices |
| US11661574B2 (en) | 2018-07-13 | 2023-05-30 | Corning Incorporated | Fluidic devices including microplates with interconnected wells |
| US11732227B2 (en) * | 2018-07-13 | 2023-08-22 | Corning Incorporated | Cell culture vessels with stabilizer devices |
| US11912968B2 (en) | 2018-07-13 | 2024-02-27 | Corning Incorporated | Microcavity dishes with sidewall including liquid medium delivery surface |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
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| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171212 |