CN107460116A - One kind is used for the preferable sperm swim-up device of sperm - Google Patents
One kind is used for the preferable sperm swim-up device of sperm Download PDFInfo
- Publication number
- CN107460116A CN107460116A CN201710774505.7A CN201710774505A CN107460116A CN 107460116 A CN107460116 A CN 107460116A CN 201710774505 A CN201710774505 A CN 201710774505A CN 107460116 A CN107460116 A CN 107460116A
- Authority
- CN
- China
- Prior art keywords
- connecting tube
- fanning strip
- sperm
- tube
- upstream
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/43—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for artificial insemination
Abstract
The invention discloses a kind of sperm swim-up device for preferred sperm, feature is upstream tube, upper connecting tube, lower connecting tube and the seminal fluid pipe for including being detachably connected successively from top to bottom, the upper end closed of upstream tube and its lower end connects with upper connecting tube, the lower end closed of seminal fluid pipe and its upper end connects with lower connecting tube, the inwall of upper connecting tube is fixedly installed the first fanning strip, and the inwall of lower connecting tube is fixedly installed second fanning strip complementary with the first fanning strip；When the first fanning strip at least partly overlaps with the second fanning strip, upper connecting tube connects with lower connecting tube, when the first fanning strip and the second fanning strip connect into circle in same level complementation, closed channel is formed between upper connecting tube and described lower connecting tube, advantage is simple in construction, good separating effect, and repeatability is high.
Description
Technical field
The present invention relates to supplementary reproduction field, is used for the preferable sperm swim-up device of sperm more particularly, to one kind.
Background technology
The sperm of high vigor is the important leverage of successful pregnancies.Therefore, it is typically right when preparing in supplementary reproduction field, sperm
Semen sample pre-processes, to filter out the higher sperm of vigor.At present in auxiliary procreation technology, generally using sperm swim-up
Method, using common 5ml round bottom test tubes, 1-2ml upstream liquid is first added, the Sperm pellets washed after centrifuging are slowly added to justify
Bottom test tube bottom；Or 1ml seminal fluid is placed in test tube bottom, in ullage addition 1-2ml upstream liquid；Or careful
1ml seminal fluid is added to the lower section of upstream liquid, pays attention to keeping the interface of liquid.Test tube tilts 45 °, is placed in 37 DEG C of incubations
30-60 minutes, upper strata upstream liquid is taken to obtain the sperm of high vigor.
The problem of traditional upper reaches method there may be as described above has：Requirement of the separating layer to operator is higher, it is possible to create
Bubble, it is easy to seminal fluid-nutrient solution separating layer is confused, or directly takes lower floor's seminal fluid to upper strata, it is final so as to influence
Separating effect, because skills involved in the labour are different between operator, the separating effect presentation with a semen sample may be caused
Notable difference, the final fertilization process for influenceing sperm and ovum, and the development being fertilized on embryo may have potential influence.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of simple in construction, good separating effect, and repeatability is high to be used for
The preferable sperm swim-up device of sperm.
Technical scheme is used by the present invention solves above-mentioned technical problem：A kind of sperm swim-up for preferred sperm fills
Put, including upstream tube, upper connecting tube, lower connecting tube and the seminal fluid pipe being detachably connected successively from top to bottom, described upstream tube
Upper end closed and its lower end connected with described upper connecting tube, the lower end closed of described seminal fluid pipe and its upper end with it is described
Lower connecting tube connection, the inwall of described upper connecting tube are fixedly installed the first fanning strip, and the inwall of described lower connecting tube is consolidated
Surely it is provided with the second fanning strip complementary with the first described fanning strip；When the first described fanning strip is fan-shaped with described second
Piece at least partly overlap when, described upper connecting tube connects with described lower connecting tube, when the first described fanning strip with it is described
The second fanning strip when same level complementation connects into circle, shape between described upper connecting tube and described lower connecting tube
Into closed channel.
Between described upstream tube and described upper connecting tube and between described seminal fluid pipe and described lower connecting tube
It is connected through a screw thread.
The lower end inner wall of described upstream tube is provided with upstream tube internal thread, and the upper end outer wall of described upper connecting tube is set
There is upper connecting tube external screw thread, described upstream tube internal thread is connected with described upper connecting tube external screw thread；Described lower company
The lower end outer wall of adapter is provided with lower connecting tube external screw thread, and the upper end inwall of described seminal fluid pipe is provided with seminal fluid pipe internal thread,
Described lower connecting tube external screw thread is connected with described seminal fluid pipe screw-internal thread fit.
The lower end outer wall equidistantly distributed of described upper connecting tube has N to enclose outer snap ring, in the upper end of described lower connecting tube
Wall equidistantly distributed have N enclose in snap ring, in the neck that described outer snap ring active card is formed between adjacent described interior snap ring,
The first described fanning strip is arranged on the bottom of the outer snap ring described in bottom, and the second described fanning strip is arranged on bottom institute
In the neck stated, the outer snap ring just inlay card described in bottom between the second described fanning strip and described interior snap ring, its
Middle N values are 3,4 or 5.When upper connecting tube and lower connecting tube is not fully connected, two connecting tubes can flexible rotating, can adjust
Fan-shaped contact area adjusts aisle spare size；When upper connecting tube and lower connecting tube are in completely enclosed connection, two connect
Adapter is fully sealed, it is impossible to rotates.
Described outer snap ring and the surface of described interior snap ring are smooth circular arc camber, and the internal diameter of described interior snap ring is small
External diameter in described outer snap ring and the internal diameter more than described outer snap ring.
The mouth of pipe of described upstream tube is additionally provided with upstream tube lid, and the outer wall of described upstream tube lid is provided with and institute
The upstream tube lid external screw thread that the upstream tube screw-internal thread fit stated uses.
The profile of described upstream tube is round bottom taper, and volume 0.5-1.5ml, internal diameter is 5-10 millimeters, is highly 30-
40 millimeters, described seminal fluid pipe is identical with the size shape of described upstream tube, described upper connecting tube and described lower connection
The height of pipe is 8-10 millimeters.
The thickness of the first described fanning strip is 0.5-1.0 millimeters and its fan angle is 180 ° ± 10 °, described second
Fanning strip is identical with the thickness of the first described fanning strip.Increase experiment sample contact area to greatest extent.
The outer wall of described upper connecting tube is provided with the groove mark for indicating sector channel opening or closed mode.
Described upstream tube, described upper connecting tube, described lower connecting tube, described seminal fluid pipe and described upstream tube
The material of lid is polypropylene, makrolon or polymethyl methacrylate.
Compared with prior art, the advantage of the invention is that：A kind of sperm swim-up device for preferred sperm of the invention,
Including upstream tube, seminal fluid pipe, connection upstream tube, the connecting tube of seminal fluid pipe and the lid of upstream tube.Upstream tube, seminal fluid pipe and company
Take over to be threadedly coupled.Before connection, the sector region being vertically connected between pipe is closure state.Into seminal fluid pipe added with certain body
After long-pending seminal fluid, by upstream tube, two connecting tubes, seminal fluid pipe connects in sequence, reversely rotates two connecting tubes, the company of opening
Connect road so that upstream liquid and seminal fluid are in Maximum Contact area, tilt 45 ° to contact surface direction, 37 DEG C are incubated 30-60 points
Clock, interface channel is closed, is slowly inverted, the upstream tube mouth of pipe upward, removes connecting tube, changes upstream tube lid, and it utilizes sperm
The principle that the principle of upstream, i.e. sperm itself can move about, the sperm of high vigor is divided from low vitality or unvital sperm
Separate out and, so as to significantly improve the success rate of inseminatio externalis (IVF) and Intracytoplasmic Sperm Injection (ICSI), avoid grasping
Inferior separating effect caused by existing manual operation during work, or the problem of separating effect differs.Its advantage is as follows：
(1) in the present invention after sperm swim-up, sector channel is closed, so that it may by the upstream liquid containing high motile sperm and seminal fluid sample
Originally separate, instead of in normal experiment, draw sperm this operation behind upstream.And avoid in routine operation, operator
Because the qualification of operation is different, easily by seminal fluid-nutrient solution, this separating layer confuses, and influences final separating effect；Simultaneously
Avoid with a semen sample, because operation is different, obvious difference is presented in separating effect.
(2) the upstream liquid of certain volume is pre-loaded with upstream tube of the present invention, it is possible to reduce after drawing upstream liquid and upstream
Seminal fluid this operation, simplify experimental implementation, help to reduce the pollution brought by manual operation in experimentation.
(3) present invention is simple to operate, requires relatively low to the qualification of operator, easily forms standardized assay operation rule
Journey, reduces the qualification because of operator, causes conspicuousness to influence experimental result.
(4) present invention is injection molding shaping, and cost is relatively low.The material of the present invention is polypropylene, makrolon, poly- methyl
In methyl acrylate it is therein any one, property is stable, and material is transparent, and sterilizing is convenient, it is easier to produces in batches, while conduct
Disposable consumptive material, reduce the possibility of experiment cross pollution.
Brief description of the drawings
Fig. 1 is the dimensional structure diagram that the present invention is used for the preferable sperm swim-up device of sperm；
Fig. 2 is the dimensional structure diagram of the upper connecting tube of the present invention；
Fig. 3 is the dimensional structure diagram of the lower connecting tube of the present invention；
Fig. 4 is that the present invention uses schematic diagram one for the preferable sperm swim-up device of sperm；
Fig. 5 is that the present invention uses schematic diagram two for the preferable sperm swim-up device of sperm；
Fig. 6 is that the present invention uses schematic diagram three for the preferable sperm swim-up device of sperm.
1-upstream tube；2-upstream tube internal thread；3-upstream tube lid；4-upstream tube lid external screw thread；5-above connection
Pipe external screw thread；6-go up connecting tube；7-the first fanning strip；8-outer snap ring；9-interior snap ring；10-lower connecting tube；11-the second
Fanning strip；12-lower connecting tube external screw thread；13-seminal fluid pipe internal thread；14-seminal fluid pipe, 15-neck, 16-groove mark.
Embodiment
The present invention is described in further detail below in conjunction with accompanying drawing embodiment.
Specific embodiment
A kind of sperm swim-up device for preferred sperm, as shown in figure 1, including being detachably connected successively from top to bottom
Upstream tube 1, upper connecting tube 6, lower connecting tube 10 and seminal fluid pipe 14, the upper end closed of upstream tube 1 and its lower end connect with upper connecting tube 6
Logical, the lower end closed of seminal fluid pipe 14 and its upper end connects with lower connecting tube 10, the inwall of upper connecting tube 6 is fixedly installed the first fan
Shape piece 7, the inwall of lower connecting tube 10 are fixedly installed second fanning strip 11 complementary with the first fanning strip 7；When the first fanning strip 7
When at least partly being overlapped with the second fanning strip 11, upper connecting tube 6 connects with lower connecting tube 10, when the first fanning strip 7 and the second fan
Shape piece 11 forms closed channel when same level complementation connects into circle between upper connecting tube 6 and lower connecting tube 10.
In this particular embodiment, as shown in Figure 1, Figure 2 and Figure 3, between upstream tube 1 and upper connecting tube 6 and seminal fluid pipe
It is connected through a screw thread between 14 and lower connecting tube 10.The lower end inner wall of upstream tube 1 is provided with upstream tube internal thread 2, upper connection
The upper end outer wall of pipe 6 is provided with connecting tube external screw thread 5, and upstream tube internal thread 2 is connected with upper connecting tube external screw thread 5；Under
The lower end outer wall of connecting tube 10 is provided with lower connecting tube external screw thread 12, and the upper end inwall of seminal fluid pipe 14 is provided with seminal fluid pipe internal thread
13, lower connecting tube external screw thread 12 is connected with seminal fluid pipe internal thread 13.
In this particular embodiment, as shown in Figures 2 and 3, the lower end outer wall equidistantly distributed of upper connecting tube 6 has N circles outer
Snap ring 8, the upper end inwall equidistantly distributed of lower connecting tube 10 have N enclose in snap ring 9, the active card of outer snap ring 8 adjacent interior snap ring 9 it
Between in the neck 15 that is formed.First fanning strip 7 is arranged on the bottom of bottom outer snap ring 8, and the second fanning strip 11 is arranged on most lower
Hold in neck 15, the just inlay card of bottom outer snap ring 8 is between the second fanning strip 11 and interior snap ring 9.Outer snap ring 8 and interior snap ring 9
Surface be smooth circular arc camber, the internal diameter of interior snap ring 9 is less than the external diameter of outer snap ring 8 and more than the internal diameter of outer snap ring 8, wherein
N values are 3,4 or 5.
In this particular embodiment, as shown in figure 1, the mouth of pipe of upstream tube 1 is additionally provided with upstream tube lid 3, upstream tube pipe
The outer wall of lid 3 is provided with the upstream tube lid external screw thread 4 being used cooperatively with upstream tube internal thread 2.The profile of upstream tube 1 is round bottom
Taper, volume 0.5-1.5ml, internal diameter are 5-10 millimeters, are highly 30-40 millimeters, the size shape of seminal fluid pipe 14 and upstream tube 1
Shape is identical.The height of upper connecting tube 6 and lower connecting tube 10 is 8-10 millimeters.The thickness of first fanning strip 7 is 0.5-1.0 millimeters
And its fan angle is 180 ° ± 10 °, the second fanning strip 11 is identical with the thickness of the first fanning strip 7.The outer wall of upper connecting tube 6 is set
It is equipped with the groove mark 16 for indicating sector channel opening or closed mode.
In this particular embodiment, upstream tube 1, upper connecting tube 6, lower connecting tube 10, seminal fluid pipe 14 and upstream tube lid 3
Material is any one in polypropylene, makrolon and polymethyl methacrylate.
Application method：As Figure 4-Figure 6, upper connecting tube 6 and lower connecting tube 10 are connected, sample passes through rotation before experiment
Fan angle, both the first fanning strip 7 and the second fanning strip 11 is set to be complementarily shaped to circular and close interface channel., will before experiment
Be preinstalled with the upstream liquid of certain volume upstream tube 1 remove upstream tube lid 3 after, upstream tube 1 and upper connecting tube 6 and lower connecting tube
The closure connecting pipe of 10 compositions is connected.Add the semen sample of certain volume into seminal fluid pipe 14, pass through screw thread and lower connecting tube
10 are connected.Now, from top to bottom, upstream tube 1, upper connecting tube 6, lower connecting tube 10, seminal fluid pipe 14 are by being in turn connected into closing
Passage.During experiment, connecting tube 6 is pulled up, the outer snap ring 8 of upper connecting tube 6 enters the top neck 15 of lower connecting tube 10
In, after upper connecting tube 6 is reversely rotated into certain angle, make two fan-shaped overlapping areas maximum, interface channel opening.By 30 points
Behind the upstream of clock or so, the higher sperm of vigor can be by swimming in the liquid of upstream on interface channel.After the completion of experiment, connect in rotation
After the certain angle of adapter 6, connecting tube 6 is pressed, the just inlay card of outer snap ring 8 is between the interior fanning strip 11 of snap ring 9 and second, and first
Both fanning strips 11 of fanning strip 7 and second are complementarily shaped to circular and close interface channel, and change upstream tube lid 3, now on
Include the sperm by upstream in 1 in play pipe, available for centrifuging, the subsequent operation such as freeze.
Described above is not limitation of the present invention, and the present invention is also not limited to the example above.The art it is common
Technical staff is in the essential scope of the present invention, the variations, modifications, additions or substitutions made, should also belong to the protection of the present invention
Scope.
Claims (10)
- A kind of 1. sperm swim-up device for preferred sperm, it is characterised in that：Including what is be detachably connected successively from top to bottom Upstream tube, upper connecting tube, lower connecting tube and seminal fluid pipe, the upper end closed of described upstream tube and its lower end and described upper connection Pipe connects, the lower end closed of described seminal fluid pipe and its upper end connects with described lower connecting tube, described upper connecting tube it is interior Wall is fixedly installed the first fanning strip, and the inwall of described lower connecting tube is fixedly installed and the first described fanning strip complementation Second fanning strip；When the first described fanning strip at least partly overlaps with the second described fanning strip, described upper connecting tube Connected with described lower connecting tube, when the first described fanning strip is connected with the second described fanning strip in same level complementation During into circle, closed channel is formed between described upper connecting tube and described lower connecting tube.
- A kind of 2. sperm swim-up device for preferred sperm according to claim 1, it is characterised in that：Described upstream It is connected through a screw thread between pipe and described upper connecting tube and between described seminal fluid pipe and described lower connecting tube.
- A kind of 3. sperm swim-up device for preferred sperm according to claim 2, it is characterised in that：Described upstream The lower end inner wall of pipe is provided with upstream tube internal thread, and the upper end outer wall of described upper connecting tube is provided with connecting tube external screw thread, Described upstream tube internal thread is connected with described upper connecting tube external screw thread；The lower end outer wall of described lower connecting tube is set There is lower connecting tube external screw thread, the upper end inwall of described seminal fluid pipe is provided with seminal fluid pipe internal thread, the outer spiral shell of described lower connecting tube Line is connected with described seminal fluid pipe screw-internal thread fit.
- A kind of 4. sperm swim-up device for preferred sperm according to claim 3, it is characterised in that：Described upper company The lower end outer wall equidistantly distributed of adapter has N to enclose outer snap ring, and the upper end inwall equidistantly distributed of described lower connecting tube has in N circles Snap ring, in the neck that described outer snap ring active card is formed between adjacent described interior snap ring, the first described fanning strip is set Put and be arranged in the bottom of the outer snap ring described in bottom, the second described fanning strip in the neck described in bottom, bottom Described outer snap ring just inlay card is between the second described fanning strip and described interior snap ring, and wherein N values are 3,4 or 5.
- A kind of 5. sperm swim-up device for preferred sperm according to claim 4, it is characterised in that：Described wild card The surface of ring and described interior snap ring is smooth circular arc camber, and the internal diameter of described interior snap ring is less than the outer of described outer snap ring Footpath and the internal diameter for being more than described outer snap ring.
- A kind of 6. sperm swim-up device for preferred sperm according to claim 5, it is characterised in that：Described upstream The mouth of pipe of pipe is additionally provided with upstream tube lid, and the outer wall of described upstream tube lid is provided with matches somebody with somebody with described upstream tube internal thread Close the upstream tube lid external screw thread used.
- A kind of 7. sperm swim-up device for preferred sperm according to claim 1-6, it is characterised in that：Described is upper The profile of play pipe is round bottom taper, and volume 0.5-1.5ml, internal diameter is 5-10 millimeters, is highly 30-40 millimeters, described essence Liquid pipe is identical with the size shape of described upstream tube, and the height of described upper connecting tube and described lower connecting tube is 8-10 Millimeter.
- A kind of 8. sperm swim-up device for preferred sperm according to claim 7, it is characterised in that：Described first The thickness of fanning strip is 0.5-1.0 millimeters and its fan angle is 180 ° ± 10 °, the second described fanning strip and described first The thickness of fanning strip is identical.
- A kind of 9. sperm swim-up device for preferred sperm according to claim 8, it is characterised in that：Described upper company The outer wall of adapter is provided with the groove mark for indicating sector channel opening or closed mode.
- A kind of 10. sperm swim-up device for preferred sperm according to claim 9, it is characterised in that：Described is upper Play pipe, described upper connecting tube, described lower connecting tube, the material of described seminal fluid pipe and described upstream tube lid are poly- third Alkene, makrolon or polymethyl methacrylate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710774505.7A CN107460116A (en) | 2017-08-31 | 2017-08-31 | One kind is used for the preferable sperm swim-up device of sperm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710774505.7A CN107460116A (en) | 2017-08-31 | 2017-08-31 | One kind is used for the preferable sperm swim-up device of sperm |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107460116A true CN107460116A (en) | 2017-12-12 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710774505.7A Pending CN107460116A (en) | 2017-08-31 | 2017-08-31 | One kind is used for the preferable sperm swim-up device of sperm |
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| Country | Link |
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| CN (1) | CN107460116A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109082363A (en) * | 2018-07-23 | 2018-12-25 | 上海市第人民医院 | A kind of preferred sperm freezing culture device |
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|---|---|---|---|---|
| US20040238123A1 (en) * | 2003-05-22 | 2004-12-02 | Axcelis Technologies, Inc. | Plasma apparatus, gas distribution assembly for a plasma apparatus and processes therewith |
| CN204589173U (en) * | 2015-04-24 | 2015-08-26 | 遵义医学院附属医院 | A kind of sperm swim-up test tube |
| CN206258199U (en) * | 2016-12-26 | 2017-06-16 | 中国人民解放军***联勤部油料监督处 | A kind of nonpetroleum temperature measuring box |
-
2017
- 2017-08-31 CN CN201710774505.7A patent/CN107460116A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040238123A1 (en) * | 2003-05-22 | 2004-12-02 | Axcelis Technologies, Inc. | Plasma apparatus, gas distribution assembly for a plasma apparatus and processes therewith |
| WO2004107414A2 (en) * | 2003-05-22 | 2004-12-09 | Axcelis Technologies Inc. | Plasma apparatus, gas distribution assembly for a plasma apparatus and processes therewith |
| CN204589173U (en) * | 2015-04-24 | 2015-08-26 | 遵义医学院附属医院 | A kind of sperm swim-up test tube |
| CN206258199U (en) * | 2016-12-26 | 2017-06-16 | 中国人民解放军***联勤部油料监督处 | A kind of nonpetroleum temperature measuring box |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109082363A (en) * | 2018-07-23 | 2018-12-25 | 上海市第人民医院 | A kind of preferred sperm freezing culture device |
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