CN107446702A - A kind of extracting method of wormwood essential oil - Google Patents

A kind of extracting method of wormwood essential oil Download PDF

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Publication number
CN107446702A
CN107446702A CN201710688423.0A CN201710688423A CN107446702A CN 107446702 A CN107446702 A CN 107446702A CN 201710688423 A CN201710688423 A CN 201710688423A CN 107446702 A CN107446702 A CN 107446702A
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Prior art keywords
wormwood
essential oil
extracting method
temperature
wormwood essential
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Pending
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CN201710688423.0A
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Chinese (zh)
Inventor
孙政
孙剑峰
时灵美
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Anhui Agricultural Science And Technology Co Ltd Chu Ai
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Anhui Agricultural Science And Technology Co Ltd Chu Ai
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Priority to CN201710688423.0A priority Critical patent/CN107446702A/en
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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A61K36/282—Artemisia, e.g. wormwood or sagebrush
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/022—Refining
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/025—Recovery by solvent extraction

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Biotechnology (AREA)
  • Public Health (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Medical Informatics (AREA)
  • Botany (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Extraction Or Liquid Replacement (AREA)
  • Fats And Perfumes (AREA)

Abstract

The present invention relates to a kind of method for extracting natural plant essence oil, more precisely, it is a kind of extracting method of wormwood essential oil, comprises the following steps：(1) fresh wormwood stem, leaf are cleaned and soaked, be then charged into basket and be integrally immersed into liquid nitrogen and handle, lifted juxtaposition 5 10min at room temperature, be then immersed into liquid nitrogen and handle again, so repeatedly 23 times；(2) by the wormwood low temperature drying in step (1), crushing, wormwood powder is obtained；(3) supercritical CO is used2Extraction extracts to wormwood powder；(4) CO containing extract2Flowed out from extraction kettle, carry out decompression separation into separating still, obtain wormwood essential oil；The extracting method of wormwood essential oil provided by the invention, avoids the loss of active ingredient in wormwood extraction process, improves the quality and yield of wormwood essential oil.

Description

A kind of extracting method of wormwood essential oil
Technical field
It is a kind of carrying for wormwood essential oil more precisely the present invention relates to a kind of method for extracting natural plant essence oil Take method.
Background technology
Wormwood essential oil is a kind of volatile flavor extracted from the leaf of wormwood, stalk, generally pale yellow or green Yellow, there is warming and activating meridian, qi and activate blood circulation, cold-dispelling pain-relieving, improvement local circulation, increase immunity, be usually used in smearing Acupuncture point or affected part, repeatedly rubbing to absorption.The composition of wormwood essential oil based on small molecule volatile terpenoid, in addition with Aliphatic compound, small molecule aromatic compound.
In the prior art, hydrodistillation, solvent extraction, supercritical fluid extraction, open steam distillation, microwave radiation technology Extraction process has been applied in the extraction research of P-Cymene, and ultrasonic extraction technique and semi-bionic extraction method technology are in folium artemisiae argyi essence Using less in oil extract research.Hydrodistillation and solvent extraction inevitably cause the change of component, so as to change Whole fragrance profile, and head perfume is usually lost, product loses feeling of freshness, and the pollution of dissolvent residual is also difficult to avoid that.And surpass and face Boundary CO2Extraction compensate for the deficiency of above-mentioned technology.Supercritical CO2Abstraction technique is built upon the CO under supercriticality2Fluid pair Organic matter has the novel separation technology in special solubility effect.Insoluble big point for traditional separation method of the technology The separation of son amount, thermal sensitivity or chemically unstable material has the advantages of unique, thus is led in food, spices, medicine, chemical industry etc. Widely paid attention in domain.
The content of the invention
For the problems of the prior art, it is an object of the invention to provide a kind of extracting method for wormwood essential oil, Avoid the loss of the active ingredient in wormwood processing procedure.
To achieve these goals, the present invention is achieved using following technical scheme：A kind of extraction side of wormwood essential oil Method, comprise the following steps：
(1) fresh wormwood stem, leaf are cleaned and the 20-30min that soaks, are then charged into basket, basket is integrally immersed into 30-60min in liquid nitrogen, then lift juxtaposition 5-10min at room temperature, then basket is integrally immersed into 30- in liquid nitrogen again 60min, so repeatedly 2-3 times；
(2) by the wormwood low temperature drying in step (1), then crush, cross 5-30 mesh sieves；Obtain wormwood powder；
(3) inserted after wormwood powder is well mixed with solvent in extraction kettle, supercritical CO is passed through into extraction kettle2, control The temperature of extraction kettle processed is 40-50 DEG C, extracting pressure 30-45Mpa；
(4) CO containing extract2Flowed out from extraction kettle, decompression separation is carried out into separating still, wherein, separating still Pressure is 5-10Mpa, and temperature is 20-30 DEG C, obtains wormwood essential oil.
It is immersed into by the wormwood cauline leaf that will be enriched in water in the liquid nitrogen of low temperature, the water freezing in cell liquid, volume becomes greatly simultaneously Cell membrane is punctured, promotes the active ingredient in wormwood cell to separate out, is then dried under conditions of low temperature, it is ensured that wormwood Middle active ingredient will not be lost in.According to the present invention, the technique of described cryogenic vacuum drying is：Wormwood is interlocked into pile in vacuum In drying box, it is 0.009-0.36Mpa to control vacuum, and vacuum drying temperature is 40-52 DEG C；
Preferably, described wormwood is dried to moisture content as less than 5%；
According to the present invention, in order to further separate out active ingredient in wormwood, the wormwood powder in the present invention inserts extraction Take and fully mixed thoroughly with the 0.01-0.08 times of biology enzyme weighed before kettle and stand 30-60min, by biology enzyme to wormwood cell membrane Decomposed, it is preferred that heretofore described biology enzyme is at least one in beta-glucosidase, pectase and cellulase Kind.
According to the present invention, solvent is removed for the ease of follow-up, solvent of the present invention is low boiling point solvent, it is preferred that Described solvent is at least one of acetone, ethanol, ether, methanol and dichloromethane.
According to the present invention, the pressure of heretofore described separating still is 7-9Mpa, and temperature is 25-30 DEG C.
Compared with prior art, the extracting method of wormwood essential oil provided by the invention, effectively avoid wormwood and extracted The loss of active ingredient in journey, improve the quality and yield of wormwood essential oil.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below Specific embodiment is closed, the present invention is furture elucidated.
Embodiment 1
A kind of extracting method of wormwood essential oil, comprises the following steps：
(1) fresh wormwood stem, leaf are cleaned and the 25min that soaks, are then charged into basket, basket is integrally immersed into liquid nitrogen Middle 50min, then lift juxtaposition 8min at room temperature, then basket is integrally immersed into 50min in liquid nitrogen again, so repeatedly 3 It is secondary；
(2) the wormwood cryogenic vacuum in step (1) is dried, then crushes, crosses 20 mesh sieves；Obtain wormwood powder；
Described cryogenic vacuum stoving process is：Wormwood is interlocked into pile in vacuum drying chamber, controls the vacuum to be 0.12Mpa, vacuum drying temperature are 45 DEG C, and wormwood is dried to moisture content as less than 5%；
(3) wormwood powder and 0.05 times of heavy beta-glucosidase are fully mixed thoroughly and stands 50min, then mixed with acetone Inserted after closing uniformly in extraction kettle, supercritical CO is passed through into extraction kettle2, the temperature for controlling extraction kettle is 45 DEG C, extracting pressure For 40Mpa；
(4) CO containing extract2Flowed out from extraction kettle, decompression separation is carried out into separating still, wherein, separating still Pressure is 8Mpa, and temperature is 28 DEG C, obtains wormwood essential oil.
Embodiment 2
A kind of extracting method of wormwood essential oil, comprises the following steps：
(1) fresh wormwood stem, leaf are cleaned and the 20min that soaks, are then charged into basket, basket is integrally immersed into liquid nitrogen Middle 30min, then lift juxtaposition 5min at room temperature, then basket is integrally immersed into 30min in liquid nitrogen again, so repeatedly 2 It is secondary；
(2) the wormwood cryogenic vacuum in step (1) is dried, then crushes, crosses 5 mesh sieves；Obtain wormwood powder；
Described cryogenic vacuum stoving process is：Wormwood is interlocked into pile in vacuum drying chamber, controls the vacuum to be 0.009Mpa, vacuum drying temperature are 40 DEG C, and wormwood is dried to moisture content as less than 5%；
(3) wormwood powder and 0.01 times of heavy pectase are fully mixed thoroughly and stands 30min, be then well mixed with ethanol After insert in extraction kettle, supercritical CO is passed through into extraction kettle2, the temperature for controlling extraction kettle is 40 DEG C, and extracting pressure is 30Mpa；
(4) CO containing extract2Flowed out from extraction kettle, decompression separation is carried out into separating still, wherein, separating still Pressure is 5Mpa, and temperature is 20 DEG C, obtains wormwood essential oil.
Embodiment 3
A kind of extracting method of wormwood essential oil, comprises the following steps：
(1) fresh wormwood stem, leaf are cleaned and the 30min that soaks, are then charged into basket, basket is integrally immersed into liquid nitrogen Middle 60min, then lift juxtaposition 10min at room temperature, then basket is integrally immersed into 60min in liquid nitrogen again, so repeatedly 3 It is secondary；
(2) the wormwood cryogenic vacuum in step (1) is dried, then crushes, crosses 30 mesh sieves；Obtain wormwood powder；
Described cryogenic vacuum stoving process is：Wormwood is interlocked into pile in vacuum drying chamber, controls the vacuum to be 0.36Mpa, vacuum drying temperature are 52 DEG C, and wormwood is dried to moisture content as less than 5%；
(3) wormwood powder and 0.08 times of heavy cellulase are fully mixed thoroughly and stands 60min, then mixed with ether Inserted after even in extraction kettle, supercritical CO is passed through into extraction kettle2, the temperature for controlling extraction kettle is 50 DEG C, and extracting pressure is 45Mpa；
(4) CO containing extract2Flowed out from extraction kettle, decompression separation is carried out into separating still, wherein, separating still Pressure is 10Mpa, and temperature is 30 DEG C, obtains wormwood essential oil.
General principle, principal character and the features of the present invention of the present invention has been shown and described above.The technology of the industry Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the simply explanation described in above-described embodiment and specification is originally The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes Change and improvement is both fallen within the range of claimed invention.The scope of protection of present invention is by appended claims And its equivalent thereof.

Claims (6)

  1. A kind of 1. extracting method of wormwood essential oil, it is characterised in that：Comprise the following steps：
    (1) fresh wormwood stem, leaf are cleaned and the 20-30min that soaks, are then charged into basket, basket is integrally immersed into liquid nitrogen Middle 30-60min, then lift juxtaposition 5-10min at room temperature, then basket be integrally immersed into 30-60min in liquid nitrogen again, So 2-3 times repeatedly；
    (2) by the wormwood low temperature drying in step (1), then crush, cross 5-30 mesh sieves；Obtain wormwood powder；
    (3) inserted after wormwood powder is well mixed with solvent in extraction kettle, supercritical CO is passed through into extraction kettle2, control extraction The temperature of kettle is 40-50 DEG C, extracting pressure 30-45Mpa；
    (4) CO containing extract2Flowed out from extraction kettle, decompression separation is carried out into separating still, wherein, the pressure of separating still For 5-10Mpa, temperature is 20-30 DEG C, obtains wormwood essential oil.
  2. 2. the extracting method of wormwood essential oil according to claim 1, it is characterised in that：In step (3), wormwood powder is put Fully mixed thoroughly with the 0.01-0.08 times of biology enzyme weighed before entering extraction kettle and stand 30-60min, wherein, described biology enzyme is At least one of beta-glucosidase, pectase and cellulase.
  3. 3. the extracting method of wormwood essential oil according to claim 1, it is characterised in that：In described step (2), wormwood warp Cryogenic vacuum is dried, and its technique is：Wormwood is interlocked into pile in vacuum drying chamber, it is 0.009-0.36Mpa to control vacuum, Vacuum drying temperature is 40-52 DEG C.
  4. 4. the extracting method of wormwood essential oil according to claim 1, it is characterised in that：In described step (2), wormwood is dried It is less than 5% to do to moisture content.
  5. 5. the extracting method of wormwood essential oil according to claim 1, it is characterised in that：Described solvent is that low boiling is molten Agent, it is preferred that described solvent is at least one of acetone, ethanol, ether, methanol and dichloromethane.
  6. 6. the extracting method of wormwood essential oil according to claim 1, it is characterised in that：In described step (4), separating still Pressure be 7-9Mpa, temperature is 25-30 DEG C.
CN201710688423.0A 2017-08-12 2017-08-12 A kind of extracting method of wormwood essential oil Pending CN107446702A (en)

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108165366A (en) * 2018-03-07 2018-06-15 安徽心缘康生物科技有限公司 A kind of extracting method of peony seed oil
CN108456594A (en) * 2018-05-07 2018-08-28 南召留山岭南艾叶制品有限公司 A kind of extracting method of high yield wormwood essential oil
CN108619410A (en) * 2018-06-14 2018-10-09 山东融基健康管理股份有限公司 A kind of Mt. Ai spirit Ai Aicao essential oils and its extraction process
CN109453353A (en) * 2018-12-21 2019-03-12 卢照宇 A kind of moxa-moxibustion essential oil and preparation method thereof
CN110591822A (en) * 2019-09-23 2019-12-20 青岛利和生物科技有限公司 Artemisia argyi essential oil extraction method for improving active component content
CN110655990A (en) * 2019-10-08 2020-01-07 宫鹏 Preparation method and application of traditional Chinese medicine wormwood essential oil
CN111019763A (en) * 2019-12-27 2020-04-17 南洋金鳄（成都）生物工程有限公司 Artemisia argyi extraction method without toxic and side effects
CN111621366A (en) * 2020-07-08 2020-09-04 山东中医药大学 Preparation method of wormwood extract with sterilization and insect expelling functions
CN111617124A (en) * 2020-07-08 2020-09-04 山东中医药大学 Preparation method of edible wormwood extract
CN112322390A (en) * 2020-10-20 2021-02-05 安徽省泰林生态农业科技发展有限公司 Method for extracting essential oil of sequoia zhongshanensis
CN114410380A (en) * 2021-12-23 2022-04-29 湖南德悦生态农业开发有限公司 Method for extracting essential oil from folium artemisiae argyi

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104162314A (en) * 2014-08-26 2014-11-26 极粹健康产业股份有限公司 Extraction process of plant effective components and application of extraction process of plant effective components
CN105296143A (en) * 2015-12-04 2016-02-03 威海皓菲集团有限公司 Preparation method of artemisia argyi essential oil
CN106243106A (en) * 2016-09-23 2016-12-21 成都合盛生物技术有限公司 A kind of method extracting tabersonine from the volt health seeds of trees

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104162314A (en) * 2014-08-26 2014-11-26 极粹健康产业股份有限公司 Extraction process of plant effective components and application of extraction process of plant effective components
CN105296143A (en) * 2015-12-04 2016-02-03 威海皓菲集团有限公司 Preparation method of artemisia argyi essential oil
CN106243106A (en) * 2016-09-23 2016-12-21 成都合盛生物技术有限公司 A kind of method extracting tabersonine from the volt health seeds of trees

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108165366A (en) * 2018-03-07 2018-06-15 安徽心缘康生物科技有限公司 A kind of extracting method of peony seed oil
CN108456594A (en) * 2018-05-07 2018-08-28 南召留山岭南艾叶制品有限公司 A kind of extracting method of high yield wormwood essential oil
CN108619410A (en) * 2018-06-14 2018-10-09 山东融基健康管理股份有限公司 A kind of Mt. Ai spirit Ai Aicao essential oils and its extraction process
CN109453353A (en) * 2018-12-21 2019-03-12 卢照宇 A kind of moxa-moxibustion essential oil and preparation method thereof
CN109453353B (en) * 2018-12-21 2021-09-28 卢照宇 Moxibustion essential oil and preparation method thereof
CN110591822A (en) * 2019-09-23 2019-12-20 青岛利和生物科技有限公司 Artemisia argyi essential oil extraction method for improving active component content
CN110655990A (en) * 2019-10-08 2020-01-07 宫鹏 Preparation method and application of traditional Chinese medicine wormwood essential oil
CN111019763A (en) * 2019-12-27 2020-04-17 南洋金鳄（成都）生物工程有限公司 Artemisia argyi extraction method without toxic and side effects
CN111621366A (en) * 2020-07-08 2020-09-04 山东中医药大学 Preparation method of wormwood extract with sterilization and insect expelling functions
CN111617124A (en) * 2020-07-08 2020-09-04 山东中医药大学 Preparation method of edible wormwood extract
CN112322390A (en) * 2020-10-20 2021-02-05 安徽省泰林生态农业科技发展有限公司 Method for extracting essential oil of sequoia zhongshanensis
CN114410380A (en) * 2021-12-23 2022-04-29 湖南德悦生态农业开发有限公司 Method for extracting essential oil from folium artemisiae argyi

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Application publication date: 20171208