CN107439795B - Feed additive containing composite seaweed and preparation process thereof - Google Patents
Feed additive containing composite seaweed and preparation process thereof Download PDFInfo
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Abstract
The invention discloses a feed additive containing compound seaweed, which contains brown algae saccharide, green algae saccharide, yeast cell wall, chitosan and other active substances, and can effectively remove the pollution of feed raw materials such as corn, grains and the like and various mycotoxins such as aflatoxin, zearalenone, T2 toxin, vomitoxin, fumonisin and the like in the feed after compounding, prevent livestock and poultry from causing various diseases due to eating the feed polluted by the mycotoxins, ensure the health of animals and improve the production benefit. The product has the advantages that firstly, the harm of mycotoxin can be completely prevented; secondly, the components play a synergistic role, the detoxification effect is strong, desorption is not easy after detoxification, and toxin removal is more thorough; thirdly, the detoxification efficiency is high, and the dosage is less; fourthly, the specificity is strong, the mycotoxin is only acted on, the nutrient elements are not adsorbed, and the normal growth of the livestock and poultry is not influenced; fifthly, the composition can enhance the immune system, improve the immune function of livestock and poultry and enhance the disease resistance of organisms.
Description
Technical Field
The invention relates to a feed additive containing composite seaweed and a preparation method and an application method thereof, belonging to the field of feed additives.
Background
Investigations from global, national and local provinces have shown that mycotoxin contamination in feed is very common and severe. Mycotoxin pollution exists in various feed raw materials, various compound feed products, various regions and various seasons, the detection rate can reach 70-100%, and the toxin exceeding rate determined according to the feed sanitation standard can reach 20-80%. The detection toxins of the polluted feed are 2-3 in a small amount and 7-8 in a large amount, and the characteristic of multiple mycotoxin combined pollution is presented. Mycotoxins affect animal product safety through both direct and indirect effects. The direct action is that mycotoxin is remained in animal food such as meat, eggs, milk and the like, and the indirect action is that the disease resistance of animals polluted by the mycotoxin is reduced, the using amount of antibiotics is increased, and the risk of the antibiotics remaining in animal products is increased. According to the data of the Food and Agricultural Organization (FAO) of the United nations, about 25 percent of crops are polluted by mycotoxin to different degrees every year in the world, and about 2 percent of crops lose feeding value due to serious pollution.
The harm of mycotoxin chronic poisoning is to destroy the immune function of animals, reduce the resistance to stress and pathogen and reduce the protection rate of vaccines. The mycotoxin is taken to cause the animal thymus to develop badly and shrink, the generation of complement C4 and lymphocyte is inhibited, the secretion of cell factor is reduced, and the mycotoxin with high dose can directly cause immunosuppression. Therefore, in the case of mycotoxin contamination, once the animal is attacked by pathogenic microorganisms, the disease is easily outbreaked.
Currently, the best control method for mycotoxins is to add mold removal agents to the feed. The commonly used mold removing agents include clay, yeast cell wall, microorganism, enzymolysis and Chinese herbal medicine. It has been found that fucoidan and chlorella extracted from seaweed and further processed have the effect of effectively inhibiting the toxic action of mycotoxin on organism, and the sugar in these seaweed has synergistic effect when used together with the above-mentioned several mildew-removing agents. Although the prior market also has a plurality of mildew removers with compound components, the mildew removers do not have corresponding national detection standards, and the use effect is greatly different. Therefore, the development of a novel, efficient and safe mold remover is more necessary.
Disclosure of Invention
The invention aims to provide a feed additive containing composite seaweed, which can effectively remove the pollution of feed raw materials such as corn, grains and the like and various mycotoxins such as aflatoxin, zearalenone, T2 toxin, vomitoxin, fumonisin and the like in the feed, prevent various diseases of livestock and poultry caused by eating the mycotoxin-polluted feed, ensure the health of animals and improve the production benefit.
The purpose of the invention is realized by the following technical scheme:
the invention provides a feed additive containing compound seaweed, which consists of brown algae saccharide, green algae saccharide, yeast cell wall, modified montmorillonite, chitosan, calcium formate, attapulgite with palygorskite mineral as the main component, sepiolite, hydrated sodium calcium aluminosilicate, copper sulfate, sodium salt and compound vitamin.
Wherein the weight ratio of the raw materials is as follows: 1-40 parts of brown algae saccharide, 1-40 parts of green algae saccharide, 1-40 parts of yeast cell wall, 10-60 parts of modified montmorillonite, 1-15 parts of chitosan, 1-40 parts of calcium formate, 0.1-40 parts of attapulgite mainly comprising palygorskite minerals, 0.1-40 parts of sepiolite, 0.1-40 parts of hydrated sodium calcium aluminosilicate, 0.01-10 parts of copper sulfate, 0.01-10 parts of sodium salt and 4-60 parts of vitamin complex, wherein the vitamin complex comprises the following components in parts by weight: 1-50 parts of vitamin A, 1-50 parts of vitamin E, 1-30 parts of vitamin C and 1-50 parts of selenium yeast.
Further, the weight ratio of the raw materials is as follows: 10-30 parts of brown algae saccharide, 5-20 parts of green algae saccharide, 1-25 parts of yeast cell wall, 18-35 parts of modified montmorillonite, 5-10 parts of chitosan, 5-30 parts of calcium formate, 0.1-30 parts of attapulgite mainly comprising palygorskite minerals, 0.1-20 parts of sepiolite, 0.1-20 parts of hydrated sodium calcium aluminosilicate, 0.01-5 parts of copper sulfate, 0.01-6 parts of sodium salt and 5-50 parts of compound vitamin, wherein the compound vitamin comprises the following components in parts by weight: 5-40 parts of vitamin A, 10-50 parts of vitamin E, 5-30 parts of vitamin C and 15-50 parts of selenium yeast.
Further, the weight ratio of the raw materials is as follows: 15-30 parts of brown algae saccharide, 10-20 parts of green algae saccharide, 10-25 parts of yeast cell wall, 18-30 parts of modified montmorillonite, 5-8 parts of chitosan, 5-20 parts of calcium formate, 0.1-20 parts of attapulgite mainly comprising palygorskite minerals, 5-20 parts of sepiolite, 5-20 parts of hydrated sodium calcium aluminosilicate, 0.01-3 parts of copper sulfate, 0.01-4 parts of sodium salt and 5-35 parts of compound vitamin, wherein the compound vitamin comprises the following components in parts by weight: 5-30 parts of vitamin A, 10-40 parts of vitamin E, 10-30 parts of vitamin C and 15-40 parts of selenium yeast.
Furthermore, the weight ratio of the raw materials is as follows: 20 parts of brown algae saccharide, 16 parts of green algae saccharide, 10 parts of yeast cell wall, 25 parts of modified montmorillonite, 5 parts of chitosan, 5 parts of calcium formate, 1 part of attapulgite mainly containing palygorskite mineral, 5 parts of sepiolite, 5 parts of hydrated sodium calcium aluminosilicate, 1.5 parts of copper sulfate, 1.5 parts of sodium salt and 5 parts of composite vitamin, wherein the composite vitamin comprises the following components in parts by weight: 30 parts of vitamin A, 25 parts of vitamin E, 30 parts of vitamin C and 15 parts of selenium yeast.
The brown algae saccharide is prepared by the following steps: adding water which is 12-18 times of the weight of brown algae powder as a raw material, uniformly stirring, boiling for 10-15min, cooling, preserving heat at 28 ℃ for 25-40min, adding alginate lyase produced by a specific strain Pseudomonas sp.HZJ216, uniformly stirring, putting the homogenate into a 28 ℃ thermostat for enzymolysis for 3h, wherein the addition of the alginate lyase is 0.05-0.08% of the weight of the homogenate, stirring continuously in the reaction process, boiling to terminate the enzymolysis reaction, and spraying and drying to obtain a finished product.
The brown algae is one or more of herba Zosterae Marinae, Undaria Pinnatifida, Sargassum and Macrocystis.
Preparation of the above green alga saccharides: washing, drying, pulverizing Chlorella powder, adding water at a ratio of 1:35, extracting at 90-100 deg.C for 4 hr, cooling, adding Chlorella polysaccharide-degrading enzyme and Mg2+Performing enzymolysis at 40-50 deg.C and pH of 7.5-8.5 for 0.5-2 hr, heating to boil and keeping the temperature for 2-3min, cooling, adding sodium dihydrogen phosphate, adjusting pH to about 7.0, and spray drying.
The green algae is one or more of Enteromorpha prolifera, Ulva lactuca and Monostroma nitidum.
The feed additive of the invention is used for removing mycotoxin from feed raw materials such as corn, grains and the like polluted by mycotoxin and compound feed, and prevents various diseases caused by the fact that livestock and poultry eat mycotoxin-polluted feed.
The invention also provides a preparation method of the feed additive, which comprises the following steps:
(1) weighing various vitamins according to the weight ratio: vitamin A, vitamin E, vitamin and selenium yeast are uniformly mixed to obtain the compound vitamin;
(2) respectively carrying out superfine grinding on the fucoidan, the chlorella, the yeast cell wall, the modified montmorillonite, the attapulgite taking palygorskite mineral as main materials, the sepiolite and the hydrated sodium calcium aluminosilicate, passing the powder through a 200-mesh sieve, and respectively weighing the fucoidan, the chlorella, the yeast cell wall, the modified montmorillonite, the chitosan, the calcium formate, the attapulgite taking palygorskite mineral as main materials, the sepiolite, the hydrated sodium calcium aluminosilicate, the copper sulfate, the sodium salt and the vitamin complex according to the weight ratio, and uniformly mixing to obtain the composite.
The invention provides an application method of the feed additive, which is characterized in that the adding proportion in the feed is 1-2 kg/T.
The invention comprehensively applies the adsorption substances, organic acid and vitamin raw materials, and the adsorption substances can strongly adsorb the mycotoxin by achieving the effect of coordinating and efficiently removing the mycotoxin and the influence thereof; the organic acid can create an acidic environment and inhibit the growth of mold; vitamins are indispensable to antibody synthesis, T cell proliferation and monocyte phagocytic function, and can resist viruses and tumors, inhibit the formation of nitroso compounds, improve the immunity of the organism, protect and repair the liver and a reproductive system, strengthen physique and relieve the injury caused by toxins.
The components and the dosage of the feed additive are obtained by the team through a large number of repeated practices, the feed additive is prepared from brown algae saccharide, chlorella saccharide, modified montmorillonite, yeast cell wall and chitosan, aiming at domestic feed raw materials and mycotoxin pollution in feed, can effectively remove the pollution of a plurality of mycotoxins such as corn, grain and other feed raw materials, aflatoxin, zearalenone, T2 toxin, vomitoxin and the like in the feed, and simultaneously protects the liver and kidney of animals, promotes toxin discharge, repairs damaged tissues and organs, improves the immunity, strengthens the physique, effectively prevents and treats mycotoxin poisoning of livestock and poultry and related diseases, and ensures the health and safety of cultured animals. The invention has the advantages and positive effects that:
(1) the feed additive is a compound mildew removing agent, and can comprehensively prevent the harm of mycotoxin. On one hand, the method can effectively remove a plurality of mycotoxins such as zearalenone, vomitoxin, T2 toxin, aflatoxin B1, fumonisin and the like in the polluted feed and prevent the mycotoxins from being absorbed in the body; on the other hand, the mycotoxin entering the animal body can be promoted to be discharged, so that the harm of the mycotoxin is avoided;
(2) the feed additive disclosed by the invention has the advantages that each component of the feed additive plays a synergistic effect, the detoxification effect is strong, the feed additive is not easy to desorb after detoxification, and the toxin is removed more thoroughly;
(3) the feed additive has high detoxification efficiency and low consumption;
(4) the feed additive has strong specificity, only acts on mycotoxin, does not adsorb nutrient elements, and does not influence the normal growth of livestock and poultry;
(5) the mildew removing agent for the feed can also enhance an immune system, improve the immune function of livestock and poultry and enhance the disease resistance of organisms.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Although the invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; such modifications and substitutions do not depart from the spirit and scope of the present invention as set forth in the appended claims.
Example 1
A feed additive containing composite seaweed is prepared by the following steps:
(1) brown algae saccharide preparation: adding water which is 12-18 times of the weight of kelp powder into the kelp powder serving as a raw material, uniformly stirring, boiling for 10-15min, cooling, preserving heat at 28 ℃ for 25-40min, adding alginate lyase produced by a specific strain Pseudomonas sp.HZJ216, uniformly stirring, putting the homogenate into a 28 ℃ thermostat for enzymolysis for 3h, wherein the addition of the enzyme is 0.05-0.08% of the weight of the homogenate, stirring continuously in the reaction process, boiling to terminate the enzymolysis reaction, and spraying and drying to obtain a finished product;
(2) preparation of green alga saccharides: washing, drying, pulverizing Ulva lactuca powder, adding water at a ratio of 1:35, extracting at 90-100 deg.C for 4 hr, cooling, adding Chlorella polysaccharide-degrading enzyme and Mg2+Performing enzymolysis at 40-50 deg.C and pH 7.5-8.5 for 0.5-2 hr, heating to boil and keeping the temperature for 2-3min after enzymolysis, cooling, adding sodium dihydrogen phosphate, adjusting pH to about 7.0, and spray drying;
(3) preparing the vitamins: weighing 30 parts of vitamin A, 25 parts of vitamin E, 20 parts of vitamin and 10 parts of yeast selenium, and uniformly mixing to obtain the compound vitamin for later use;
(4) crushing: micronizing attapulgite, sepiolite and hydrated sodium calcium aluminosilicate with brown algae saccharide, chlorella saccharide, yeast cell wall, modified montmorillonite and palygorskite mineral as main materials, and sieving with 200 mesh sieve;
(5) mixing: weighing the raw materials according to the proportion of 20 parts of brown algae saccharide, 25 parts of green algae saccharide, 12 parts of yeast cell wall, 30 parts of modified montmorillonite, 2 parts of chitosan, 2 parts of calcium formate, 0.5 part of attapulgite mainly comprising palygorskite mineral, 0.5 part of sepiolite, 0.4 part of hydrated sodium calcium aluminosilicate, 0.2 part of copper sulfate, 2.4 parts of sodium salt and 5 parts of vitamin complex respectively, and uniformly mixing to obtain the composite vitamin.
Example 2
A feed additive containing composite seaweed is prepared by the following steps:
(1) brown algae saccharide preparation: adding water which is 12-18 times of the weight of the undaria pinnatifida serving as a raw material into the undaria pinnatifida, uniformly stirring the mixture, boiling the mixture for 10-15min, cooling the mixture, keeping the temperature of the mixture at 28 ℃ for 25-40min, adding alginate lyase produced by a specific strain Pseudomonas sp.HZJ216, uniformly stirring the mixture, putting the homogenate into a thermostat at 28 ℃ for enzymolysis for 3h, wherein the addition amount of the alginate lyase is 0.05-0.08% of the weight of the homogenate, stirring the mixture continuously in the reaction process, boiling the mixture to stop the enzymolysis reaction, and spraying and drying the mixture to obtain a finished product;
(2) preparation of green alga saccharides: adding water into the enteromorpha powder according to the material-liquid ratio of 1:35, extracting at 90-100 deg.C for 4h, cooling, adding Chlorella polysaccharide degrading enzyme and Mg into the solution2+Performing enzymolysis at 40-50 deg.C and pH 7.5-8.5 for 0.5-2 hr, heating to boil and keeping the temperature for 2-3min after enzymolysis, cooling, adding sodium dihydrogen phosphate, adjusting pH to about 7.0, and spray drying;
(3) preparing the vitamins: weighing 20 parts of vitamin A, 30 parts of vitamin E, 22 parts of vitamin C and 8 parts of yeast selenium, and uniformly mixing to obtain the compound vitamin for later use;
(4) crushing: micronizing attapulgite, sepiolite and hydrated sodium calcium aluminosilicate with brown algae saccharide, chlorella saccharide, yeast cell wall, modified montmorillonite and palygorskite mineral as main materials, and sieving with 200 mesh sieve;
(5) mixing: the preparation method comprises the following steps of weighing and uniformly mixing 22 parts of brown algae saccharides, 20 parts of green algae saccharides, 15 parts of yeast cell walls, 35 parts of modified montmorillonite, 3 parts of chitosan, 3 parts of calcium formate, 0.1 part of attapulgite mainly comprising palygorskite minerals, 0.1 part of sepiolite, 0.1 part of hydrated soda-calcium aluminosilicate, 0.5 part of copper sulfate, 3 parts of sodium salt and 8 parts of vitamin complex.
Example 3
A feed additive containing composite seaweed is prepared by the following steps:
(1) brown algae saccharide preparation: adding 12-18 times of water into sargassum powder as a raw material, uniformly stirring, boiling for 10-15min, cooling, keeping the temperature at 28 ℃ for 25-40min, adding alginate lyase produced by a specific strain Pseudomonas sp.HZJ216, uniformly stirring, putting the homogenate into a 28 ℃ thermostat for enzymolysis for 3h, wherein the addition of the enzyme is 0.05-0.08% of the weight of the homogenate, stirring continuously in the reaction process, boiling to terminate the enzymolysis reaction, and spraying and drying to obtain a finished product;
(2) preparation of green alga saccharides: washing, drying, pulverizing Ulva lactuca powder, adding water at a ratio of 1:35, extracting at 90-100 deg.C for 4 hr, cooling, adding Chlorella polysaccharide-degrading enzyme and Mg2+Performing enzymolysis at 40-50 deg.C and pH 7.5-8.5 for 0.5-2 hr, heating to boil and keeping the temperature for 2-3min after enzymolysis, cooling, adding sodium dihydrogen phosphate, adjusting pH to about 7.0, and spray drying;
(3) preparing the vitamins: weighing 25 parts of vitamin A, 35 parts of vitamin E, 25 parts of vitamin C and 9 parts of yeast selenium, and uniformly mixing to obtain the compound vitamin for later use;
(4) crushing: micronizing attapulgite, sepiolite and hydrated sodium calcium aluminosilicate with brown algae saccharide, chlorella saccharide, yeast cell wall, modified montmorillonite and palygorskite mineral as main materials, and sieving with 200 mesh sieve;
(5) mixing: the preparation method comprises the following steps of weighing and uniformly mixing 22 parts of brown algae saccharides, 28 parts of green algae saccharides, 8 parts of yeast cell walls, 35 parts of modified montmorillonite, 2 parts of chitosan, 5 parts of calcium formate, 0.1 part of attapulgite mainly comprising palygorskite minerals, 0.1 part of sepiolite, 0.1 part of hydrated soda-calcium aluminosilicate, 0.5 part of copper sulfate, 3 parts of sodium salt and 15 parts of vitamin complex.
Example 4
A feed additive containing composite seaweed is prepared by the following steps:
(1) brown algae saccharide preparation: adding water which is 12-18 times of the weight of kelp powder into the kelp powder serving as a raw material, uniformly stirring, boiling for 10-15min, cooling, keeping the temperature at 28 ℃ for 25-40min, adding alginate lyase produced by a specific strain Pseudomonas sp.HZJ216, uniformly stirring, putting the homogenate into a 28 ℃ thermostat for enzymolysis for 3h, wherein the addition amount of the alginate lyase is 0.05-0.08% of the weight of the homogenate, stirring continuously in the reaction process, boiling to terminate the enzymolysis reaction, and spraying and drying to obtain a finished product;
(2) preparation of green alga saccharides: washing, drying, pulverizing Ulva lactuca powder, adding water at a ratio of 1:35, extracting at 90-100 deg.C for 4 hr, cooling, adding Chlorella polysaccharide-degrading enzyme and Mg2+Performing enzymolysis at 40-50 deg.C and pH 7.5-8.5 for 0.5-2 hr, heating to boil and keeping the temperature for 2-3min after enzymolysis, cooling, adding sodium dihydrogen phosphate, adjusting pH to about 7.0, and spray drying;
(3) preparing the vitamins: weighing 30 parts of vitamin A, 35 parts of vitamin E, 30 parts of vitamin C and 15 parts of yeast selenium, and uniformly mixing to obtain the compound vitamin for later use;
(4) crushing: micronizing attapulgite, sepiolite and hydrated sodium calcium aluminosilicate with brown algae saccharide, chlorella saccharide, yeast cell wall, modified montmorillonite and palygorskite mineral as main materials, and sieving with 200 mesh sieve;
(5) mixing: the preparation method comprises the following steps of weighing raw materials according to the proportion of 30 parts of brown algae saccharides, 35 parts of green algae saccharides, 1 part of yeast cell walls, 30 parts of modified montmorillonite, 10 parts of chitosan, 8 parts of calcium formate, 0.1 part of attapulgite mainly comprising palygorskite minerals, 0.1 part of sepiolite, 0.1 part of hydrated soda-calcium aluminosilicate, 0.5 part of copper sulfate, 5 parts of sodium salt and 25 parts of compound vitamin, and uniformly mixing the raw materials to obtain the composite pigment.
Example 5
A feed additive containing composite seaweed is prepared by the following steps:
(1) brown algae saccharide preparation: adding water which is 12-18 times of the weight of kelp powder into the kelp powder serving as a raw material, uniformly stirring, boiling for 10-15min, cooling, keeping the temperature at 28 ℃ for 25-40min, adding alginate lyase produced by a specific strain Pseudomonas sp.HZJ216, uniformly stirring, putting the homogenate into a 28 ℃ thermostat for enzymolysis for 3h, wherein the addition amount of the alginate lyase is 0.05-0.08% of the weight of the homogenate, stirring continuously in the reaction process, boiling to terminate the enzymolysis reaction, and spraying and drying to obtain a finished product;
(2) preparation of green alga saccharides: adding water into the enteromorpha powder according to the material-liquid ratio of 1:35, extracting at 90-100 deg.C for 4h, cooling, adding Chlorella polysaccharide degrading enzyme and Mg into the solution2+Performing enzymolysis at 40-50 deg.C and pH 7.5-8.5 for 0.5-2 hr, heating to boil and keeping the temperature for 2-3min after enzymolysis, cooling, adding sodium dihydrogen phosphate, adjusting pH to about 7.0, and spray drying;
(3) preparing the vitamins: weighing 35 parts of vitamin A, 25 parts of vitamin E, 25 parts of vitamin C and 20 parts of yeast selenium, and uniformly mixing to obtain the compound vitamin for later use;
(4) crushing: micronizing attapulgite, sepiolite and hydrated sodium calcium aluminosilicate with brown algae saccharide, chlorella saccharide, yeast cell wall, modified montmorillonite and palygorskite mineral as main materials, and sieving with 200 mesh sieve;
(5) mixing: weighing raw materials according to the proportion of 35 parts of brown algae saccharide, 25 parts of green algae saccharide, 5 parts of yeast cell wall, 35 parts of modified montmorillonite, 6 parts of chitosan, 6 parts of calcium formate, 0.1 part of attapulgite mainly comprising palygorskite mineral, 0.1 part of sepiolite, 0.1 part of hydrated soda-calcium aluminosilicate, 0.5 part of copper sulfate, 5 parts of sodium salt and 30 parts of vitamin complex respectively, and uniformly mixing to obtain the composite vitamin E-K-N-doped alginate.
Example 6
A feed additive containing composite seaweed is prepared by the following steps:
(1) brown algae saccharide preparation: adding water which is 12-18 times of the weight of kelp powder into the kelp powder serving as a raw material, uniformly stirring, boiling for 10-15min, cooling, keeping the temperature at 28 ℃ for 25-40min, adding alginate lyase produced by a specific strain Pseudomonas sp.HZJ216, uniformly stirring, putting the homogenate into a 28 ℃ thermostat for enzymolysis for 3h, wherein the addition amount of the alginate lyase is 0.05-0.08% of the weight of the homogenate, stirring continuously in the reaction process, boiling to terminate the enzymolysis reaction, and spraying and drying to obtain a finished product;
(2) preparation of green alga saccharides: adding water into the enteromorpha powder according to the material-liquid ratio of 1:35, extracting at 90-100 deg.C for 4h, cooling, adding Chlorella polysaccharide degrading enzyme and Mg into the solution2+Performing enzymolysis at 40-50 deg.C and pH 7.5-8.5 for 0.5-2 hr, heating to boil and keeping the temperature for 2-3min after enzymolysis, cooling, adding sodium dihydrogen phosphate, adjusting pH to about 7.0, and spray drying;
(3) preparing the vitamins: weighing 15 parts of vitamin A, 15 parts of vitamin E, 30 parts of vitamin C and 30 parts of yeast selenium, and uniformly mixing to obtain the compound vitamin for later use;
(4) crushing: micronizing attapulgite, sepiolite and hydrated sodium calcium aluminosilicate with brown algae saccharide, chlorella saccharide, yeast cell wall, modified montmorillonite and palygorskite mineral as main materials, and sieving with 200 mesh sieve;
(5) mixing: the preparation method comprises the following steps of weighing raw materials according to the proportion of 40 parts of brown algae saccharides, 35 parts of green algae saccharides, 5 parts of yeast cell walls, 30 parts of modified montmorillonite, 5 parts of chitosan, 10 parts of calcium formate, 0.1 part of attapulgite mainly comprising palygorskite minerals, 0.1 part of sepiolite, 0.1 part of hydrated soda-calcium aluminosilicate, 0.5 part of copper sulfate, 5 parts of sodium salt and 25 parts of compound vitamin, and uniformly mixing the raw materials to obtain the composite pigment.
Experiments on the detoxification effect of the feed additives of the invention on mycotoxins were carried out in the laboratory in examples 1, 2, 3, 4, 5 and 6. The experiment is divided into 8 groups, the 7 th group is a defective experiment group, and the feed additive is used for equally replacing the green alga saccharides in the example 6 with brown alga saccharides; group 8 deficient test group, the brown algae saccharide in example 6 was replaced with green algae saccharide in equal amount as feed additive. The mycotoxins are zearalenone, aflatoxin B1 (AFB 1), vomitoxin, T-2 toxin and fumonisin B1 respectively, 10ml of the mycotoxin standard solution (shown in table 1) is added into a centrifuge tube, and 25mg of the feed additive sample is added into the centrifuge tube, so that the concentration of the feed additive in the solution is 2.5 mg/ml. All samples were adsorbed in a 37 ℃ constant temperature water bath shaker with shaking at 150r/min for 2 h. Then, the mixture was centrifuged at 3000r/min for 10min, and the supernatants were collected and the content of each mycotoxin in each supernatant was determined. According to the calculation formula, the adsorption quantity Q (ng/mg) and the adsorption rate Y (%) are measured in time. Then 10ml of PSB buffer solution with pH7.0 is added into the residue, the mixture is shaken and analyzed for 30min in a thermostatic waterbath oscillator with temperature of 37 ℃ and speed of 150r/min, the mixture is centrifuged for 10min at 3000r/min, supernatant fluid is collected, and the content of each mycotoxin in each supernatant fluid is measured again. All samples were set up in 2 replicates. The cumulative resolution (%) was determined after the blank group and the control group were corrected.
The formula I is as follows: q = V (C)0-C1)/M
The formula II is as follows: y =100 (C)0-C1)/ C0
The formula III is as follows: r = C2/(C0-C1)*100%
Wherein V is the volume of the solution (ml); m is the dosage (mg) of the feed additive; c0As the concentration of various mycotoxins (ng/mg) prior to adsorption; c1As the concentration of various mycotoxins after adsorption (ng/mg); c2The concentration of each mycotoxin after desorption (ng/mg).
The results of the test are shown in Table 1.
TABLE 1 results of experiments on detoxification of various mycotoxins by the feed additive of the invention
The experimental results show that the adsorption rate of various mycotoxins in the blank control group is 0, and the adsorption rate of the medicine in the medicine control group is AFB1The adsorption rate of (A) is more than 90%, and the others are all lower than 70%; test groupThe adsorption rate for each mycotoxin is higher than that of the blank and the control group, the adsorption efficiency is from high to low in example 6, example 5, example 4, example 3, example 2 and example 1, and example 6 and example 5 are basically consistent with the adsorption efficiency for each toxin. Experiments show that the feed additive has good removal effect on zearalenone, AFB1, vomitoxin, T-2 toxin and fumonisin B1, is tightly combined with toxin, is more thorough in detoxification, and is obviously superior to the removal effect of modified montmorillonite on various mycotoxins. Furthermore, from the experimental results, the effects of adsorbing toxins of examples 5 and 6 were the best. In addition, the test result shows that the synergistic effect between the brown algae and the green algae obtains' 1+1>2 "technical effect.
Claims (5)
1. A feed additive containing compound seaweed is characterized in that the feed additive consists of attapulgite taking brown algae saccharide, chlorella saccharide, yeast cell wall, modified montmorillonite, chitosan, calcium formate and palygorskite minerals as main components, sepiolite, hydrated sodium calcium aluminosilicate, copper sulfate, sodium salt and compound vitamin, wherein the weight ratio of the raw materials is as follows: 1-40 parts of brown algae saccharide, 1-40 parts of green algae saccharide, 1-40 parts of yeast cell wall, 10-60 parts of modified montmorillonite, 1-15 parts of chitosan, 1-40 parts of calcium formate, 0.1-40 parts of attapulgite mainly comprising palygorskite minerals, 0.1-40 parts of sepiolite, 0.1-40 parts of hydrated sodium calcium aluminosilicate, 0.01-10 parts of copper sulfate, 0.01-10 parts of sodium salt and 4-60 parts of vitamin complex, wherein the vitamin complex comprises the following components in parts by weight: 1-50 parts of vitamin A, 1-50 parts of vitamin E, 1-30 parts of vitamin C and 1-50 parts of selenium yeast; the preparation process of the feed additive comprises the following steps:
(1) weighing various vitamins according to the weight ratio: vitamin A, vitamin E, vitamin and selenium yeast are uniformly mixed to obtain the compound vitamin;
(2) respectively carrying out superfine grinding on the fucoidan, the chlorella, the yeast cell wall, the modified montmorillonite, the attapulgite taking palygorskite mineral as main materials, the sepiolite and the hydrated sodium calcium aluminosilicate, passing the powder through a 200-mesh sieve, respectively weighing the fucoidan, the chlorella, the yeast cell wall, the modified montmorillonite, the chitosan, the calcium formate, the attapulgite taking palygorskite mineral as main materials, the sepiolite and the hydrated sodium calcium aluminosilicate according to the weight ratio, and uniformly mixing the attapulgite, the sepiolite and the hydrated sodium calcium aluminosilicate with copper sulfate, sodium salt and vitamin complex to obtain the composite pigment;
the brown algae saccharide is prepared by the following steps: adding water which is 12-18 times of the weight of brown algae powder as a raw material into the brown algae powder, uniformly stirring, boiling for 10-15min, cooling, keeping the temperature at 28 ℃ for 25-40min, adding alginate lyase produced by a specific strain Pseudomonas sp.HZJ216, uniformly stirring, putting the homogenate into a 28 ℃ thermostat for enzymolysis for 3h, wherein the addition of the enzyme is 0.05-0.08% of the weight of the homogenate, stirring continuously in the reaction process, boiling to terminate the enzymolysis reaction, and spraying and drying to obtain a finished product;
the preparation of the green alga saccharides comprises the following steps: washing, drying, pulverizing Chlorella powder, adding water at a ratio of 1:35, extracting at 90-100 deg.C for 4 hr, cooling, adding Chlorella polysaccharide-degrading enzyme and Mg2+Performing enzymolysis at 40-50 deg.C and pH 7.5-8.5 for 0.5-2 hr, heating to boil and keeping the temperature for 2-3min after enzymolysis, cooling, adding sodium dihydrogen phosphate, adjusting pH to 7.0, and spray drying;
the addition proportion of the feed additive in the feed is 1-2 kg/T.
2. The feed additive containing the compound seaweed as claimed in claim 1, wherein the feed additive comprises the following raw materials in parts by weight: 10-30 parts of brown algae saccharide, 5-20 parts of green algae saccharide, 1-25 parts of yeast cell wall, 18-35 parts of modified montmorillonite, 5-10 parts of chitosan, 5-30 parts of calcium formate, 0.1-30 parts of attapulgite mainly comprising palygorskite minerals, 0.1-20 parts of sepiolite, 0.1-20 parts of hydrated sodium calcium aluminosilicate, 0.01-5 parts of copper sulfate, 0.01-6 parts of sodium salt and 5-50 parts of compound vitamin, wherein the compound vitamin comprises the following components in parts by weight: 5-40 parts of vitamin A, 10-50 parts of vitamin E, 5-30 parts of vitamin C and 15-50 parts of selenium yeast.
3. The feed additive containing the compound seaweed as claimed in claim 1, wherein the feed additive comprises the following raw materials in parts by weight: 20 parts of brown algae saccharide, 16 parts of green algae saccharide, 10 parts of yeast cell wall, 25 parts of modified montmorillonite, 5 parts of chitosan, 5 parts of calcium formate, 1 part of attapulgite mainly containing palygorskite mineral, 5 parts of sepiolite, 5 parts of hydrated sodium calcium aluminosilicate, 1.5 parts of copper sulfate, 1.5 parts of sodium salt and 5 parts of composite vitamin, wherein the composite vitamin comprises the following components in parts by weight: 30 parts of vitamin A, 25 parts of vitamin E, 30 parts of vitamin C and 15 parts of selenium yeast.
4. The feed additive of claim 1, wherein the brown algae is one or more of kelp, wakame, gulfweed and kelp.
5. The feed additive containing composite seaweed as claimed in claim 1, wherein the green seaweed is one or more of Enteromorpha prolifera, Ulva lactuca and Monostroma nitidum.
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| CN110338278A (en) * | 2019-07-30 | 2019-10-18 | 威海长青海洋科技股份有限公司 | One main laminaria fermented feed containing probiotics and preparation method thereof |
| CN113519703B (en) * | 2021-07-08 | 2024-01-30 | 厦门惠盈动物科技有限公司 | Feed additive for treating mycotoxin and protecting liver and kidney for animals, preparation method and application |
| CN113845121B (en) * | 2021-10-22 | 2023-09-29 | 龙岩学院 | Modified layered aluminosilicate for improving quality of duck eggs, and preparation method and application thereof |
| CN114208968A (en) * | 2021-12-21 | 2022-03-22 | 山东绿松农业科技有限公司 | Additive of laying hen feed and preparation method and application thereof |
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